Download pdf - avaliação comparativa de teste imunocromatográfico

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P. LONGO / REVISTA BRASILEIRA DE CRIMINALSTICA, V. 1, N.1, 16-21 (2011)

1. INTRODUO

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RESUMO

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ABSTRACT

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2. MATERIAL E MTODO

2.1. Material

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2.3. Procedimento de coleta de amostras

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3.1. Procedimento de coleta de amostras

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- 15 - - - - - - *

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GD )LJ DSRQWDUDP D GHWHFomR GH VDQJXH KXPDQRQDVVROXo}HVGHFRQFHQWUDomRDWpHPSUHJDQGRVH R WHVWH GH &RRPEV WHQGRVH FRPR VROYHQWH DVROXomRVLROyJLFD H D VROXomR VDOLQD WDPSRQDGD3DUDDV FRQFHQWUDo}HV GH VDQJXH GH D RV UHVXOWDGRV IRUDP QHJDWLYRV REVHUYDQGRVH QRPLFURVFySLRySWLFRDVDJOXWLQDo}HVFDUDFWHUtVWLFDVGHVWHVUHVXOWDGRVQHJDWLYRV

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2 WHVWH )HFD&XOW DSRQWRX UHVXOWDGRV SRVLWLYRVQDVFRQFHQWUDo}HVGHDQmRGHWHFWDGDVSHOR PpWRGR VRUROyJLFR HYLGHQFLDQGR VXD PDLRUVHQVLELOLGDGH)LJ


20

Figura 8. ,OXVWUDomR GRV UHVXOWDGRV SRVLWLYRV DSRQWDGRV QD 7DEREWLGRV SHOR PpWRGR GH PHPEUDQD UHDWLYD )HFD&XOW 'D HVTXHUGDSDUD D GLUHLWD RV UHVXOWDGRV UHIHUHPVH jV FRQFHQWUDo}HV GH D

1D 7DEHOD IRUDP DSRQWDGRV RV UHVXOWDGRVSURYHQLHQWHV GRPpWRGR )HFD&XOW SDUD FRQFHQWUDo}HVDFLPD GH YH]HV GH GLOXLomR 2V UHVXOWDGRVREWLGRV DSRQWDUDP SDUD D GHWHFomR GH VDQJXH KXPDQRHP FRQFHQWUDo}HV H[WUHPDPHQWH UHGX]LGDV TXDQGRFRPSDUDGRV DRV UHVXOWDGRV REWLGRV SHOR PpWRGRVRUROyJLFR DWXDOPHQWH HPSUHJDGR SHORV 1~FOHRV GH3HUtFLDV&ULPLQDOtVWLFDV

Tabela 3.5HVXOWDGRVGDGHWHFomRGHVDQJXHKXPDQRHPGLIHUHQWHVGLOXHQWHVHFRQFHQWUDo}HViJXDGHVWLODGDVROXomRVLROyJLFDHVDOLQD

WDPSRQDGDHPSUHJDQGRVHR)HFD&XOW

Concent.Feca- Cult

gua destiladaSoluo

VLROyJLFDSalina

tamponada - - - -

SRVLWLYRQHJDWLYR

$V DPRVWUDV TXH FRQWLQKDP R VDQJXH KXPDQRGLOXtGR HP VROXomR VLROyJLFD UHYHODUDP UHVXOWDGRVSRVLWLYRV DWp D FRQFHQWUDomR GH (VWHV

UHVXOWDGRV WDPEpP IRUDP REVHUYDGRV QDV DPRVWUDVGLOXtGDV FRP VROXomR VDOLQD WDPSRQDGD IRUQHFLGD FRPR NLW 5HVVDOWDVH TXH D IDL[D SRVLWLYD GD PHPEUDQDrevelou-se mais intensa na diluio empregando-se a

VDOLQDHPFRPSDUDomRjVROXomRVLROyJLFD)LJ

Figura 9. 0HPEUDQDV UHDWLYDV )HFD&XOW UHVXOWDQWHV GD DQiOLVHGH VROXo}HV GH VDQJXH KXPDQR FRP FRQFHQWUDomR GH GLOXtGDVHPiJXDGHVWLODGDVROXomRVLROyJLFDHVROXomRVDOLQDWDPSRQDGD

2VPHOKRUHVUHVXOWDGRVIRUDPREWLGRVQDVOHLWXUDVGDV PHPEUDQDV UHDWLYDV HIHWXDGDV FRP DV DPRVWUDVGLOXtGDV HP iJXD GHVWLODGD RQGH VH REWHYH UHVXOWDGRSRVLWLYR SDUD VDQJXH KXPDQR QD FRQFHQWUDomR GH

$ OLVH GDV KHPiFLDV SHOD iJXD GHVWLODGD SRGHWHU DX[LOLDGR QD GHWHFomR GH VDQJXH KXPDQR HPFRQFHQWUDo}HV DEDL[R GH GLVSRQLELOL]DQGRPDLV KHPRJORELQD QD VROXomR GHYLGR j UXSWXUD GRVHULWUyFLWRVTXDQGRFRPSDUDGDFRPDVVROXo}HVFRQWHQGRKHPRJORELQDV tQWHJUDV GDV GLOXLo}HV HIHWXDGDV FRP DVRXWUDVGXDVVROXo}HVWHVWDGDV

&RQIRUPH )LJ QRWDVH TXH D LQWHQVLGDGHGDV FRORUDo}HV GD WD WHVWH p PXLWR VHPHOKDQWH SDUDWRGRV RV VROYHQWHV XWLOL]DGRV QD GLOXLomR DLQGD TXHD FRQFHQWUDomR GD VROXomR FRP iJXD GHVWLODGD VHMD DPHWDGHGDVGHPDLV$YDOLDomRGDLQXrQFLDGHR[LGDomRGHSHoDVVREUHRUHVXOWDGRGRVWHVWHVGH&RRPEVH)HFD&XOW

2VUHVXOWDGRVGRVWHVWHVGH&RRPEVH)HFD&XOWGD DQiOLVH GH LQXrQFLD GH R[LGDomR VREUH R UHVXOWDGRGHVWHVWHVWHVHVWmRH[SUHVVRVQD7DE

Concentraogua destilada 6ROXomR)LVLROyJLFD Salina Tamponada

Coombs Feca- Cult Coombs Feca- Cult Coombs Feca- Cult

- - - - - - - - - - - - -

Tabela 2.5HVXOWDGRVGDGHWHFomRGHVDQJXHKXPDQRHPSUHJDQGRVHFRPRGLOXHQWHiJXDGHVWLODGDVROXomRVLROyJLFDHVDOLQDWDPSRQDGDFRPSDUDQGRVHRVWHVWHVGH&RRPEVH)HFD&XOW

SRVLWLYRQHJDWLYR


21

Tabela 4.5HVXOWDGRVGDGHWHFomRGHVDQJXHKXPDQRHPDPRVWUDVR[LGDGDVHQmRR[LGDGDV

Pea Coombs Feca-Cult

/kPLQDGHDoRR[LGDGD

/kPLQDGHDoRR[LGDGD

/kPLQDGHDoRR[LGDGD -

/kPLQDGHDoRR[LGDGD

/kPLQDGHDoRR[LGDGD - -

/kPLQDGHDoRQmRR[LGDGD

/kPLQDGHDoRQmRR[LGDGD

/kPLQDGHDoRQmRR[LGDGD - -

/kPLQDGHDoRQmRR[LGDGD - -SRVLWLYRQHJDWLYR

2EVHUYDVHFRQFRUGkQFLDGHQ HQWUHRVWHVWHVGH&RRPEVH)HFD&XOW$H[FHomRVHGiQDOkPLQDR[LGDGDFXMRUHVXOWDGRGR)HFD&XOWVHUHYHORXHPWUrVminutos, mesmo tempo de revelao das amostras de

VDQJXHKXPDQRGLOXtGRHQWUHH,VVR SRGH H[SOLFDU R UHVXOWDGR QHJDWLYR GR WHVWH GH&RRPEVSDUDHVWD OkPLQDFRQVLGHUDQGRDGLIHUHQoDGHVHQVLELOLGDGH GHVWH PpWRGR TXDQGR FRPSDUDGR FRPR)HFD&XOW pSURYiYHOTXH D FRQFHQWUDomRGH VDQJXHIRVVH LQIHULRU j GHWHFWiYHO SRU&RRPEVPDV DFLPDGROLPLWHGHGHWHFomRSHOR)HFD&XOW4. CONCLUSES

2)HFD&XOW DSUHVHQWRXFRQFRUGkQFLDGHFRPRVUHVXOWDGRVREWLGRVFRPDPpWRGRSDUDGHWHFomRGHVDQJXHXWLOL]DGRURWLQHLUDPHQWHHPDQiOLVHVIRUHQVHV3RGHVHDLQGDVXJHULUTXHIRLPDLVHFD]LGHQWLFDQGRDSDUHQWHPHQWH VDQJXH KXPDQR HP FRQFHQWUDo}HV QmRGHWHFWiYHLVSHORVRXWURVPpWRGRV

'HYHVH DQDOLVDU D FRQFHQWUDomR GD VXEVWkQFLDKHPiWLFD SDUD VH HVWDEHOHFHU TXDO R VROYHQWH PDLVDGHTXDGR SDUD FDGD VLWXDomR (P FRQFHQWUDo}HV H

TXDQWLGDGHVPXLWREDL[DVGHVXEVWkQFLDRXVRGDiJXDGHVWLODGD IRUQHFH UHVXOWDGRVPDLV VHQVtYHLV FRP R XVRGR.LW)HFD&XOWDSHVDUGHDSUHVHQWDU UHVXOWDGR IDOVRQHJDWLYR QR H[DPH VRUROyJLFR DWUDYpV GR PpWRGR GH&RRPEV-iDVROXomRVLROyJLFDHDVDOLQD WDPSRQDGDSRGHPVHUXWLOL]DGDVSDUDDVGHPDLVFRQFHQWUDo}HVHPDPERVRVWHVWHV

2DoRR[LGDGRSDUHFHQmRDIHWDUR UHVXOWDGRGR)HFD&XOW R TXH QRV VXJHUH TXH R UHVXOWDGR QHJDWLYRSDUD.DVWOH0H\HUHSRVLWLYRSDUD)HFD&XOWREVHUYDGRQD FRPSDUDomR HQWUH RV WUrV WHVWHV GHYH VHU H[SOLFDGRSHODKLSyWHVHPDLVSDUFLPRQLRVDOHYDQWDGDEDVHDGDQDSRVVtYHOGLIHUHQoDGHVHQVLELOLGDGHH[LVWHQWHHQWUHHVWHVWHVWHV

'HVWH PRGR D DQiOLVH HP DPRVWUDV VXVSHLWDVde sangue depositadas em peas criminais por meio do

DQWLFRUSRDQWLKHPRJORELQDKXPDQDPRVWURXVHHFLHQWHQD GHWHFomR TXDOLWDWLYD GH KHPRJORELQD KXPDQD9DOHapontar como vantagem em relao aos demais testes

HVSHFtFRVSDUDVDQJXHKXPDQRDUDSLGH]GRUHVXOWDGRHDIDFLOLGDGHGHDSOLFDomRGRWHVWHLPXQRFURPDWRJUiFRAGRADECIMENTOS

$JUDGHFHPRVDFRODERUDomRWpFQLFDGRVVHJXLQWHV3HULWRVFULPLQDLVGR1~FOHRGH%LRORJLDH%LRTXtPLFD+HOHQD0DULD )HUUHLUD &DVWUR ,RQH GH )UHLWDV$UDXMR5LFDUGR /RSHV 2UWHJD 5RVHO\ *DOR 6RDUHV 6RQLD5HJLQD (XJrQLR 0D]]RQL 0DULD $SDUHFLGD $YHOLQR0DULD/XLVD$OPHLGD3UDGR2OLYHLUD H6RXVD5REHUWD+LUVFKIHOG &DPSRORQJR H HP HVSHFLDO j WpFQLFD GHODERUDWyULR6XHOL)HOL[GRV6DQWRV2OLYHLUD

REFERNCIAS BIBLIOGRFICAS

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