ad 50 mlEtOH

Baku PCT 50 mg

ad 10 ml

10x10 cm

2µl 4µl 6µl 8µl 10µl 4µl 4µl 4µl

CARA KERJA

Di Pipet 1 ml

Elusi : heksan : etil (1: 2)

Baca pada ƛ 254 nm

Sentrifuge 200 rpm

Pengencerean sampel

Sampel PCT~50 mg 50 mL etanol (1000 ppm)

(10 μL x 1 μg/μL = 4 μg)

Pengenceran Baku

Parasetamol ~ 50 mg 50 mL etanol (1000 ppm)

2 μL, 4 μL, 6 μL, 8 μL, 10 μl :

(2 μL x 1 μg/μL = 2 μg)

(4 μL x 1 μg/μL = 4 μg)

(6 μL x 1 μg/μL = 6 μg)

(8 μL x 1 μg/μL = 8 μg)

(10 μL x 1 μg/μL = 10 μg)

CONTOH

1 Tabel Pengamatan

Fraksi Rf Konsentrasi

totolan μg

Tinggi

puncak

Luas Area

Baku 1 0,62 1 60,26 4967,09

Baku 2 0,64 2 101,19 11927,38

Baku 3 0,65 3 122,05 15745,51

Baku 4 0,66 4 155,58 21928,36

Baku 5 0,66 5 166,46 25091,75

Asetosal 1 0,62 110,10 10709,10

Asetosal 2 0,62 75,75 7353,29

Asetosal 3 0,63 57,57 5719,38

0.5 1 1.5 2 2.5 3 3.5 4 4.5 5 5.50

5000

10000

15000

20000

25000

30000

f(x) = 5024.9 x + 856.900000000002R² = 0.985752961174625

Kosentrasi

Area

IV.2 Perhitungan

Pengencerean sampel

Sampel asetosal ~ 250 mg 50 mL etanol (5000 ppm)

1 mL 10 mL etanol (500 ppm)

4 μL

(4 μL x 0,5 μg/μL = 2 μg)

Pengenceran Baku

asetosal ~ 250 mg 50 mL etanol (5000 ppm)

1 mL 10 mL etanol (500 ppm)

4 μL

(4 μL x 0,5 μg/μL = 2 μg)

Perhitungan Kadar

y = 856,928 + 2512,515x

Asetosal 1

10709,10 = 856,928 + 2512,515x

X = 3,92 µg

%K =3,92µg2µg

x 100%

%K = 196%

Asetosal 2

7353,29 = 856,928 + 2512,515x

X = 2,5856 µg

%K =2,5856µg2µg

x 100%

%K = 129,28%

Asetosal 3

5719,38 = 856,928 + 2512,515x

X = 1,9353 µg

%K =1,9353µg2µg

x 100%

%K = 97,675%