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グローバルＣＯＥプログラム（生命科学分野）「In vivo ヒト代謝システム生物学拠点」

業績集　2007年度〜2011年度※ 2011年10月時点

末松　誠

(2007 年度 )1. Adachi, T., Yamamoto, M. & Suematsu, M. Targeting NAD(P)H oxidase:

Ets-1 regulates p47phox expression. Circ. Res. 101(10), 962-964, 2007.2. Baran, R., Robert, M., Suematsu, M., Soga, T.& Tomita, M Visualization

of three-way comparisons of omics data. BMC Bioinformatics, 8(1), 72, 2007

3. Dekio, I., Sakamoto, M., Hayashi, H., Amagai, M., Suematsu, M. & Benno, Y. Characterization of skin microbiota in patients with atopic dermatitis and in normal subjects using 16S rRNA gene-based comprehensive analysis. J. Med. Microbiol. 56(12) 1675-1683, 2007.

4. Egawa, T., Tsuneshige, A., Suematsu, M. & Yonetani, T. Method for determination of association and dissociation rate constants of reversible biomolecular reactions by isothermal titration calorimeters. Anal. Biochem. 79(7), 2972-2978, 2007.

5. Hangai-Hoger, N., Tsai, A.G., Carbales, P., Suematsu, M., & Intaglietta, M. Microvascular and systemic effects following top load administration of saturated carbon monoxide-saline solution. Crit. Care Med. 35(4), 1123-1132, 2007.

6. Higuchi, A., Ueno, R., Shimmura, S., Suematsu, M., Dogru, M. & Tsubota, K. Albumin rescues ocular epithelial cells from cell death in dry eye. Curr. Eye Res. 32(2), 83-88, 2007.

7. Kawaguchi-Manabe, H., Ieda, M., Kimura, K., Manabe, T., Miyatake, S., Kanazawa, H., Kawakami, T., Ogawa, S., Suematsu, M., & Fukuda, K. A novel cardiac hypertrophic factor, neurotrophin-3, is paradoxically downregulated in cardiac hypertrophy. Life Sci. 81(5), 385-392, 2007.

8. Kinoshita, A., Tsukada, K., Soga, T., Hishiki, T., Ueno, Y., Nakayama, Y., Tomita, M., Suematsu, M. Roles of hemoglobin allostery in hypoxia-induced metabolic alterations in erythrocytes: simulation and its verification by metabolome analysis. J. Biol. Chem. 282(14), 10731-10741, 2007.

9. Li, D., Mukai, K., Suzuki, T., Suzuki, R., Yamashita, S., Mitani, F. & Suematsu, M. Adrenocortical zonation factor 1 is a novel matricellular protein promoting integrin-mediated adhesion of adrenocortical and vascular smooth muscle cells. FEBS J. 274(10), 2506-2522, 2007.

10. Nakazato, T., Sagawa, M., Yamato, K., Xian, M., Yamamoto, T., Suematsu, M., Ikeda, Y. & Kizaki, M. Myeloperoxidase is a key regulator of oxidative stress-mediated apoptosis in myeloid leukemic cells. Clin. Cancer Res. 13(18), 5436-5445, 2007.

11. Shimaoka, T., Seino, K., Kume, N., Minami, M., Nishime, C., Suematsu, M., Kita, T., Taniguchi, M., Matsushima, K. & Yonehara, S. Critical role for CXC chemokine ligand 16 (SR-PSOX) in Th1 response mediated by NKT cells. J. Immunol. 179(12), 8172-8179, 2007.

12. Shimokata, K., Katayama, Y., Murayama, H., Suematsu, M., Tsukihara, T., Muramoto, K., Yoshikawa, S. & Shimada, H. A peptide bond provides unidirectional respiratory proton pumping in bovine cytochrome c oxidase. Proc. Natl. Acad. Sci. USA. 104(10), 4200-4205, 2007

13. Watanabe, R., Matsumura, M., Munemasa, T., Fujimaki, M. & Suematsu, M. Mechanism of hepatic parenchyma-specific contrast of microbubble-based contrast agent for ultrasonography : microscopic studies in rat liver. Invest. Radiol. 42(9), 643-651, 2007.

(2008 年度 )1. Akahoshi, N., Kobayashi, C., Ishizaka, Y., Izumi, T., Himi, T., Suematsu, M.,

Ishii, I. Genetic background conversion ameliorates semi-lethality and

permits behavioral analyses in cystathionine β-synthase-deficient mice, an animal model for hyperhomocystinemia. Hum. Mol. Genet. 17, 1994-2005, 2008.

2. Kuramori, C., Azuma, M., Kume, K., Kaneko, Y., Inoue, A., Yamaguchi, Y., Kabe, Y., Hosoya, T., Kizaki, M., Suematsu, M., Handa, H. Capsaicin binds to prohibitin 2 and displaces it from the mitochondria to the nucleus. Biochem. Biophys. Res. Commun. 379(2):519-25. 2009 Feb 6;Epub 2008 Dec 29.

3. Kuramori, C., Hase, Y., Hoshikawa, K., Watanabe, K., Nishi, T., Hishiki, T., Soga, T., Nashimoto, A., Kabe, Y., Yamaguchi, Y., Watanae, H., Kataoka, K., Suematsu, M., Handa, H. Mono-(2-ethylhexyl) phthalate targets glycogen debranching enzyme and affects glycogen metabolism in rat testis. Toxicol. Sci. 109(1): 143-51. 2009. Epub 2009 Feb 23.

4. Naito, T., Shiohara, T., Hibi, T., Suematsu, M. & Ishikawa, H. RORγτ is dispensable for the development of intestinal mucosal T cells. Mucosal Immunology 1(3), 198-207, 2008

5. Nanno, M., Kanari, Y., Naito, T., Inoue, N., Hisamatsu, T., Chinen, H., Sugimoto, K., Shimomura, Y., Yamagishi, H., Shiohara, T., Uehara, S., Matsushima, K., Suematsu, M., Mizoguchi, A., Hibi, T., Bhan, A.K., Ishikawa, H. Exacerbating role of γδ T cells in chronic colitis of T cell receptor αβ mutant mice. Gastroenterology 134(2), 481-490, 2008.

6. Ogishima, T., Mitani, F., Suematsu, M. Cytochrome P-45017a in b-cells of rat pancreas and its local steroidogenesis. J. Steroid Biochem. Mol. Biol. 111, 80-86, 2008.

7. Shimizu, N., Yoshikawa, N. Wada, T., Handa, H., Sano, M., Fukuda, K., Suematsu, M., Sawai, T., Morimoto, C., Tanaka, H. Tissue- and context-dependent modulation of hormonal sensitivity of glucocorticoid-sensitive genes by HEXIM1. Mol. Endocrinol. 22, 2609-2623, 2008

8. Suemizu, H., Hasegawa, M., Kawai, K., Taniguchi, K., Monnai, M., Wakui, M., Suematsu, M., Ito, M., Peltz, G., Nakamura, M. Establishment of a humanized model of liver using NOD/Shi-scid IL2Rγnull mice. Biochem. Biophys. Res. Commun. 377, 248-252, 2008

9. Tsugawa, H., Suzuki, H., Nakagawa, I.,Nishizawa, T., Saito, Y., Suematsu, M., Hibi, T. a-ketoglutarate oxidoreductase, an essential salvage enzyme of energy metabolism, in coccoid form of H. pylori. Biochem. Biophys. Res. Commun. 376, 46-51, 2008

10. Yukutake, Y., Tsuji, S., Hirano, Y., Adachi, T., Takahashi, T., Fujihara, K., Agre, P., Yasui, M., Suematsu, M. Mercury chloride decreases the water permeability of aquapolin-4-reconstituted proteoliposomes. Biol. Cell 100, 355-363, 2008

11. Shintani, T., Iwabuchi, T., Soga, T., Kato, Y., Yamamoto, T., Takano, N., Hishiki, T., Ueno, Y., Ikeda, S., Sakuragawa, T., Ishikawa, K., Goda, N., Kitagawa, Y., Kajimura, M., Matsumoto, K., Suematsu, M. Cystathionine β-synthase as a carbon monoxide-sensitive regulator of bile excretion. Hepatology 49, 141-150, 2009.

(2009 年度 )1. Tajima T, Goda N, Fujiki N, Hishiki T, Nishiyama Y, Senoo-Matsuda N,

Shimazu M, Soga T, Yoshimura Y, Johnson RS, Suematsu M. HIF-1α is necessary to support gluconeogenesis during liver regeneration. Biochem Biophys Res Commun 387(4), 789-794, 2009.

2. Nishino T, Yachie-Kinoshita A, Hirayama A, Soga T, Suematsu M, Tomita M. In silico modeling and metabolome analysis of long-stored erythrocytes to improve blood storage methods. J Bioengineer 144(3), 212-223, 2009.

3. Yoshikawa N, Nagasaki M, Sano M, Tokudome S, Ueno K, Shimizu N, Imoto S, Miyano S, Suematsu M, Fukuda K, Morimoto C, Tanaka

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H. Ligand-based gene expression profiling reveals novel roles of glucocorticoid receptor in cardiac metabolism. Am J Physiol Endocrinol Metab 296, E1363-E1373, 2009.

4. Harada T, Yuba-Kubo A, Sugiura Y, Zaima N, Hayasaka T, Goto-Inoue N, Wakui M, Suematsu M, Takeshita K, Ogawa K, Yoshida Y, Setou M. Visualization of volatile substances in different organelles with an atmospheric-pressure mass microscope. Analyt Chem 81(21), 9153-9157, 2009.

5. Endo J, Sano M, Katayama T, Hishiki T, Shinmura K, Morizane S, Matsuhashi T, Katsumata Y, Zhang Y, Ito H, Nagahata Y, Marchitti S, Nishimaki K, Wolf AM, Nakanishi H, Hattori F, Vasiliou V, Adachi T, Ohsawa I, Taguchi R, Hirabayashi Y, Ohta S, Suematsu M, Ogawa S, Fukuda K. Metabolic remodeling induced by mitochondrial aldehyde stress stimulates tolerance to oxidative stress in the heart. Circ Res 105(11), 1118-27, 2009.

6. Katayama T, Sano M, Endo J, Hishiki T, Nagahata Y, Tokudome S, Shinmura K, Nishimaki K, Matsuhashi T, Katsumata Y, Morizane S, Ito H, Takehana K, Cavener DR, Ohsawa I, Ohta S, Suematsu M, Fukuda K. Intracellular depletion of histidine mediates stress response to aldehydes. Circ Res 105(11), 1118-1127, 2009.

7. Yukutake Y, Hirano Y, Suematsu M, Yasui M. Rapid and reversible inhibition of aquaporin-4 by zinc. Biochemistry 48(51), 12059-12061, 2009.

8. Tajiri Y, Igarashi T, Li D, Mukai K, Suematsu M, Fukui E, Yoshizawa M, Matsumoto H. Tubulointerstitial nephritis antigen-like 1 is expressed in extraembryonic tissues and interacts with laminin 1 in the reichert membrane at post-implantation in the mouse. Biol Reprod 82(2), 263-270, 2010.

9. Sato D, Kobayashi S, Yasui H, Shibata N, Toru T, Yamamoto M, Tokoro G, Ali V, Soga T, Takeuchi T, Suematsu M, Nozaki T. Cytotoxic effect of amide derivatives of trifluoromethionine against the enteric protozoan parasite Entamoeba histolytica. Int J Antimicrob Agents 35(1), 56-61, 2010.

10. Kondo K, Sugioka T, Tsukada K, Aizawa M, Takizawa M, Shimizu K, Morimoto M, Suematsu M, Goda N. Fenofibrate, a peroxisome proliferator-activated receptor α agonist, improves hepatic microcirculatory patency and oxygen availability in a high-fat-diet-induced fatty liver in mice. Adv Exp Med Biol. 662:77-82, 2010.

11. Morikawa T, Hattori K, Kajimura M, Suematsu M. The Effects of cilostazol on tissue oxygenation upon an ischemic-reperfusion injury in the mouse cerebrum. Adv Exp Med Biol. 662:89-94, 2010.

12. Takano N, Yamamoto T, Adachi T, Suematsu M. Assessing a shift of glucose biotransformation by LC-MS/MS-based metabolome analysis in carbon monoxide-exposed cells. Adv Exp Med Biol. 662:101-107, 2010.

13. Akatsu T, Tsukada K, Hishiki T, Suga-Numa K, Tanabe M, Shimazu M, Kitagawa Y, Yachie-Kinoshita A, Suematsu M. T-state stabilization of hemoglobin by nitric oxide to form α-nitrosyl heme causes constitutive release of ATP from human erythrocytes. Adv Exp Med Biol. 662, 109-114, 2010.

14. Handa K, Ohmura M, Nishime C, Hishiki T, Nagahata Y, Kawai K, Suemizu H, Nakamura M, Wakui M, Kitagawa Y, Suematsu M, Tsukada K. Phosphorescence-assisted microvascular O2 measurements reveal alterations of oxygen demand in human metastatic colon cancer in the liver of superimmunodeficient NOG mice. Adv Exp Med Biol. 662, 423-429, 2010.

15. Kurihara T, Kubota Y, Ozawa Y, Takubo K, Noda K, M. Simon C, Johnson RS, Suematsu M, Tsubota K, Ishida S, Goda N, Suda T, and Okano H. von Hippel-Lindau protein regulates transition from fetal to adult circulatory system in retina. Development 137(9), 1563-1571, 2010.

16. Sakuragawa T, Hishiki T, Ueno Y, Ikeda S, Soga T, Yachie-Kinoshita A, Kajimura M, Suematsu M. Hypotaurine is an energy-saving hepatoprotective compound against ischemia-reperfusion injury of the rat liver. J Clin Biochem Nutr 46(2), 126-134, 2010.

17. Hirano Y, Okimoto N, Kadohira I, Suematsu M, Yasuoka K, Yasui M. Molecular mechanisms of how mercury inhibits water permeation through aquaporin-1: understanding by molecular dynamics simulation. Biophys J 98(8): 1512-1519, 2010.

(2010 年度 )1. Katayama Y, Shimokata K, Suematsu M, Ogura T, Tsukihara S,

Yoshikawa S, Shimada H. Cell-free synthesis of cytochrome c oxidase,

a multicomponent membrane protein. J Bioenerg Biomembr 2010, Jun;42(3):235-40.Epub 2010 Apr 6.

2. Handa K, Ohmura M, Nishime C, Hishiki T, Nagahata Y, Kawai K, Suemizu H, Nakamura M, Wakui M, Kitagawa Y, Suematsu M, Tsukada K. Phosphorescence-assisted microvascular O2 measurements reveal alterations of oxygen demand in human metastatic colon cancer in the liver of superimmunodeficient NOG mice. Adv Exp Med Biol. 662, 423-429, 2010.

3. Kajimura M, Fukuda R, Bateman RM, Yamamoto T, Suematsu M. Interactions of multiple gas-transducing systems: Hallmarks and uncertainties of CO, NO and H2S gas biology. Antioxid Redox Signal 13(2), 157-192, 2010.

4. Kurihara T, Kubota Y, Ozawa Y, Takubo K, Noda K, M. Simon C, Johnson RS, Suematsu M, Tsubota K, Ishida S, Goda N, Suda T, and Okano H. von Hippel-Lindau protein regulates transition from fetal to adult circulatory system in retina. Development 137(9), 1563-1571, 2010.

5. Hattori K, Kajimura M, Hishiki T, Nakanishi T, Kubo A, Nagahata Y, Ohmura M, Yachie-Kinoshita, Matsuura T, Morikawa T, Nakamura T, Setou M, Suematsu M. Paradoxical ATP elevation in ischemic penumbra revealed by quantitative imaging mass spectrometry. Antioxid Redox Signal (News & Views), 13(8), 1157-1167, 2010.

6. Yamamoto T, Shimizu Y, Ueda T, Shiro Y, Suematsu M. Application of micro-reactor chip technique to millisecond quenching of deuterium incorporation into 70S ribosomal protein complex. Int J Mass Spectr In press.

7. Tsunaga H, Suzuki H, Satoh K, Hirata K, Matsuzaki J, Saito Y, Suematsu M, Hibi T. Two amino acids mutation of ferric uptake regulator (Fur) determines Helicobacter pylori resistance to metronidazole. Antioxid Redox Signal 14, 15-23, 2011.

8. Takubo K, Goda N, Yamada W, Iriuchishima H, Ikeda E Kubota Y, Shima H Johnson RS, Hirao A, Suematsu M, Suda T . Regulation of the HIF-1α level is essential for hematopoietic stem cells. Cell Stem Cell ,7(3),391-402, 2010.

9. Ishii I, Akahoshi N, Yamada H, Nakano S, Izumi T, Suematsu M. Cystathionine γ-lyase-deficient mice require dietary cysteine to protect against acute lethal myopathy and oxidative injury. J Biol Chem , 285(34), 26358-26368, 2010.

10. Jeelani G, Husain A, Sato D, Ali V, Suematsu M, Soga T, Nozaki T. Two atypical L-cysteine-regulated NADPH-dependent oxidoreductases involved in redox maintenance and metronidazole activation in the enteric protozoon Entamoeba histolytica. J Biol Chem, 285(35), 26889-26899, 2010.

11. Zhang Y, Sano M, Shinmura K, Tamaki K, Katsumata Y, Matsuhashi T, Morizane S, Ito H, Hishiki T, Endo J, Zhou H, Yuasa S, Kaneda R, Suematsu M, Fukuda K. 4-hydroxy-2-nonenal protects against cardiac ischemia-reperfusion injury via the Nrf2-dependent pathway. J Mol Cell Cardiol , 49(4), 576-586, epub 2010.

(2011 年度 )1. Yamamoto T, Takano N, Ishiwata K, Suematsu M. Carbon monoxide

stimulates global protein methylation via its inhibitory action on cystathionine β-synthase. J Clin Biochem Nutr 48(1), 96-100, 2011.

2. Hasegawa M, Kawai K, Mitsui T, Taniguchi K, Monnai M, Wakui M, Ito M, Suematsu M, Peltz G, Nakamura M, Suemizu H. The reconstituted ʻHumanized liverʼ in TK-NOG mice is mature and functional. Biochem Biophys Res Commun , 405(3), 405-410, 2011.

3. Shimizu N, Yoshikawa N, Ito N, Maruyama T, Suzuki Y, Takeda S, Nakae J, Tagata Y, Nishitani S, Takehana K, Sano M, Fukuda K, Suematsu M, Morimoto C, Tanaka H. Crosstalk between glucocorticoid receptor and nutritional sensor mTOR in skeletal muscle. Cell Metabolism ,13(2), 170-182, 2011.

4. Ishimoto T, Nagano O, Yae T, Tamada M, Motohara T, Oshima H, Oshima M, Ikeda T, Asaba R, Yagi H, Masuko T, Shimizu T, Ishikawa T, Kai K, Takahashi E, Imamura Y, Baba Y, Ohmura M, Suematsu M, Baba H, Saya H. CD44 variant regulates redox status in cancer cells by stabilizing the xCT subunit of system xc- and thereby promotes tumor growth. Cancer Cell , 19(3), 387-400, 2011.

5. Ikeda K, Kubo A, Akahoshi N, Yamada H, Miura N, Hishiki T, Nagahata Y, Matsuura T, Suematsu M, Taguchi R, Ishii I. Triacylglycerol/phospholipid molecular species profiling of fatty livers and regenerated non-fatty

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livers in cystathionine beta-synthase-deficient mice, an animal model for homocysteinemia/homocystinuria. Anal Bioanal Chem. 2011 Jun;400(7):1853-63. Epub 2011 Feb 8.

6. Husain A, Sato D, Jeelani G, Mi-ichi F, Ali V, Suematsu M, Soga T, Nozaki T. Metabolome analysis revealed increase in S-methylcysteine and phosphatidylisopropanolamine synthesis upon L-cysteine deprivation in the anaerobic protozoan parasite Entamoeba histolytica. J Biol Chem. 2010 Dec 10;285(50):39160-70. Epub 2010 Oct 5.

7. Nakamura T, Kajimura M, Morikawa T, Hattori K, Ishikawa M, Yukutake Y, Uchiyama SI, Suematsu M. Acute CO2-independent vasodilatation of penetrating and pre-capillary arterioles in mouse cerebral parenchyma upon hypoxia revealed by a thinned-skull window method.Acta Physiol (Oxf). 2011 Sep;203(1):187-96. doi: 10.1111/j.1748-1716.2010.02212.x. Epub 2010 Dec 7.

8. Soga T, Sugimoto M, Honma M, Mori M, Igarashi K, Kashikura K, Ikeda S, Hirayama A, Yamamoto T, Yoshida H, Otsuka M, Tsuji S, Yatomi Y, Sakuragawa T, Watanabe H, Nihei K, Saito T, Kawata S, Suzuki H, Tomita M, Suematsu M. Serum metabolomics reveals γ-glutamyl dipeptides as biomarkers for discrimination among different forms of liver disease. J Hepatol. 2011 Oct;55(4):896-905. Epub 2011 Feb 18.

9. Kubo A, Ohmura M, Wakui M, Harada T, Kajihara S, Ogawa K, Suemizu H, Nakamura M, Setou M, Suematsu M. Semi-quantitative analyses of metabolic systems of human colon cancer metastatic xenografts in livers of superimmunodeficient NOG mice. Anal Bioanal Chem. 2011 Jun;400(7):1895-904. Epub 2011 Apr10.

10. Nishiyama Y, Goda N, Kanai M, Niwa D, Osanai K, Yamamoto Y, Senoo-Matsuda N, Johnson RS, Miura S, Kabe Y, Suematsu M. HIF-1α induction suppresses excessive lipid accumulation in alcoholic fatty liver in mice. J Hepatol. 2011 Sep 5. [Epub ahead of print]

11. Ochiai D, Goda N, Hishiki T, Kanai M, Senoo-Matsuda N, Soga T, Johnson RS, Yoshimura Y, Suematsu M. Disruption of HIF-1 α in hepatocytes impairs glucose metabolism in diet-induced obesity mice. Biochem Biophys Res Commun. 2011 Oct 22. [Epub ahead of print]

12. Ikeda K, Kubo A, Akahoshi N, Yamada H, Miura N, Hishiki T, Nagahata Y, Matsuura T, Suematsu M, Taguchi R, Ishii I. Triacylglycerol/phospholipid molecular species profiling of fatty livers and regenerated non-fatty livers in cystathionine beta-synthase-deficient mice, an animal model for homocysteinemia/homocystinuria. Anal Bioanal Chem. 2011 Jun;400(7):1853-63. Epub 2011 Feb 8.

13. Morikawa T, Kajimura M, Nakamura T, Hishiki T, Nakanishi T, Yukutake Y, Nagahata Y, Ishikawa M, Hattori K, Takenouchi T, Takahashi T, Ishii I, Matsubara K, Kabe Y, Uchiyama S, Nagata E, Gadalla MM, Snyder SH, Suematsu M. Hypoxic regulation of the cerebral microcirculation is mediated by a carbon monoxide-sensitive hydrogen sulfide pathway. PNAS. 2012 in press.

安井正人

(2007 年度 )1. Nagase H, Agren J, Saito A, Liu K, Agre P, Hazama A, and Yasui M.

Molecular cloning and characterization of mouse aquaporin-6. Biochem. Biophys. Res. Commun. 352:12-16 (2007)

2. Sasaki Y, Tsubota K, Kawedia J.D, Menon A.G, and Yasui M. The Difference of Aquaporin 5 Distribution in Acinar and Ductal Cells in lacrimal and parotid glands. Curr. Eye Res. 32(11):923-929 (2007)

(2008 年度 )1. Kadohira I, Abe Y, Nuriya M, Sano K, Tsuji S, Arimitsu T, Yoshimura Y,

and Yasui M. Phosphorylation in the C-terminal domain of Aquaporin-4 is required for Golgi transition in primary cultured astrocytes. Biochem Biophys Res Commun. 377(2):463-468 (2008)

2. Yukutake Y, Tsuji S, Hirano Y, Adachi T, Takahashi T, Fujihara K, Agre P, and Yasui M. (corresponding) and Suematsu M: Mercury chloride decreases the water permeability of aquaporin-4–reconstituted proteoliposomes. Biol Cell. 100(6):355–363 (2008)

(2009 年度 )1. Yasui M. pH regulated anion permeability of aquaporin-6. Handb Exp

Pharmacol. 190: 299-308 (2009)

2. Narumi S, Muroya K, Abe Y, Yasui M, Asakura Y, Adachi M, and Hasegawa T. TSHR mutations as a cause of congenital hypothyroidism in Japan: a population-based genetic epidemiology study. J Clin Endocrinol Metab. 94:1317-23 (2009)

3. Rabaud N.E, Song L, Wang Y, Agre P, Yasui M, and Carbrey J.M. Aquaporin 6 binds calmodulin in a calcium-dependent manner. Biochem Biophys Res Commun. 383:54-7 (2009)

4. Tatsumi K, Tsuji S, Miwa H, Morisaku T, Nuriya M, Orihara M, Kaneko K, Okano H, and Yasui M. Drosophila big brain does not act as a water channel, but mediates cell adhesion. FEBS Lett. 583:2077-82 (2009)

5. Arimitsu T, Nuriya M, Ikeda K, Takahashi T. and Yasui M. Activity-dependent regulation of HCN1 protein in cortical neurons. Biochem Biophys Res Commun. 387:87-91 (2009)

6. Laforenza U, Gastaldi G, Polimeni M, Tritto S, Tosco M, Ventura U, Scaffino M.F. and Yasui M. Aquaporin-6 is expressed along the rat gastrointestinal tract and upregulated by feeding in the small intestine. BMC Physiol. 9:1-12 (2009)

7. Kobayashi K, Inai T, and Yasui M. Dynamic changes in amniotic tight junctions during pregnancy. Placenta. 10:840-7 (2009)

8. Yukutake Y, Hirano Y, Suematsu M, and Yasui M. Rapid and Reversible Inhibition of Aquaporin-4 by Zinc. Biochemistry. 48(51):12059-61 (2009)

9. Kobayashi K, Kadohira I, Tanaka M, Yoshimura Y, Ikeda K, and Yasui M. Expression and distribution of tight junction proteins in human amnion during late pregnancy. Placenta. 31(2):158-62 (2009)

10. Ito T, Nuriya M, and Yasui M. Regulation of Kv2.1 phosphorylation in an animal model of anoxia. Neurobiol Dis. 38(1):85-91 (2010)

(2010 年度 )1. Yukutake Y, and Yasui M. Regulation of water permeability through

aquaporin-4. Neuroscience. 168(4):885-91 (2010)2. Hirano Y, Okimoto N, Yasuoka K, Taiji M, Suematsu M, and Yasui M.

Molecular Mechanisms How Mercury Inhibits Water Permeation of Aquaporin-1: Understanding by Molecular Dynamics Simulation. Biophys J. 21:98(8):1512-9 (2010)

3. Nuriya M, and Yasui M. Membrane potential dynamics of axons in cultured hippocampal neurons probed by second harmonic generation imaging. J Biomed Opt. 15(2):020503 (2010)

4. Matsuzaki J, Suzuki H, Minegishi Y, Sugai E, Tsugawa H, Yasui M, and Hibi T. Acid Suppression by Proton Pump Inhibitors Enhances Aquaporin-4 and KCNQ1 Expression in Gastric Fundic Parietal Cells in Mouse. Dig Dis Sci. 55(12):3339-48 (2010)

5. Kobayashi K. and Yasui M. Cellular and subcellular localization of aquaporins 1, 3, 8, and 9 in amniotic membranes during pregnancy in mice. Cell Tissue Res. 342:307-316 (2010)

6. Kobayashi K, Miwa H, Yasui M. Inflammatory mediators weaken the amniotic membrane barrier through disruption of tight junctions. J Physiol. 588:4859-4869 (2010)

7. Kobayashi K, Umezawa K, and Yasui M. Apoptosis in mouse amniotic epithelium is induced by activated macrophages through the TNF receptor type 1/TNF pathway. Biol Reprod. 84:248-54 (2011)

（2011 年度）1. Yu YC, Miki H, Nakamura Y, Hanyuda A, Matsuzaki Y, Abe Y, Yasui M,

Tanaka K, Hwang TC, Bompadre SB, and Sohma Y. Curcumin and genistein additively potentiate G551D-CFTR. J Cyst Fibros. 10(4):243-52 (2011)

2. Akai M, Onai K, Kusano M, Sato M, Redestig H, Toyooka K, Morishita M, Miyake H, Hazama A, Checchetto V, Szabo I, Matsuoka K, Saito K, Yasui M, Ishiura M, and Uozumi N. Plasma membrane aquaporin AqpZ is essential for glucose metabolism during photomixotrophic growth of Synechocystis sp. PCC 6803. J Biol Chem. 286(28):25224-35 (2011)

3. Akimoto T, Yamamoto E, Yasuoka K, Hirano Y, and Yasui M. Non-Gaussian Fluctuations Resulting from Power-Law Trapping in a Lipid Bilayer. Phys. Rev. Lett. in press (2011)

4. Ibata K, Takimoto S, Morisaku T, Atsushi Miyawaki, and Yasui M. Analysis of Aquaporin-Mediated Diffusional Water Permeability by Coherent Anti-Stokes Raman Scattering Microscopy. Biophys J. In press (2011)

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梅澤一夫

(2007 年度 )1. R. Kitamura, T. Ogata, Y. Tanaka, K. Motoyoshi, M. Seno, I. Takei, K.

Umezawa and I. Kojima: Conophylline and betacellulin-δ4: an effective combination of differentiation factors for pancreatic β cells. Endocline J. 54: 255-264, 2007.

2. M. Watanabe, M. Z. Dewan, M. Taira, M. Shoda, M. Honda, T. Sata, M. Higashihara, M. E. Kadin, T. Watanabe, N. Yamamoto, K. Umezawa and R. Horie: IκBα independent induction of NF-κB and its inhibition by DHMEQ in Hodgkin/Reed-Sternberg cells. Laboratory Investigation 87: 372-382, 2007.

3. Kimura, Y. N., Watari, K., Fotovati1, A., Hosoi1, F., Yasumoto, K., Izumi, H., Kohno, K., Umezawa, K., Iguchi, H., Shirouzu, K., Takamori, S., Kuwano, M. & Ono, M.: Inflammatory stimuli from macrophages and cancer cells synergistically promote tumor growth and angiogenesis. Cancer Science 98: 2009-2018, 2007.

4. Kimura. N., Miyakawa, Y, Kohmura. K, Umezawa. K., Ikeda, Y. & Kizaki, M.: Targeting NF-κB and induction of apoptosis by novel NF-κB inhibitor, dehydroxymethylepoxyquinomicin (DHMEQ) in Burkitt lymphoma cells. Leukemia Research, 31: 1529-1535, 2007.

5. Komi, Y., Ohno, O., Suzuki, Y., Shimamura, M., Shimokado, K., Umezawa, K. & Kojima, S.: Inhibition of tumor angiogenesis by targeting endothelial ATP synthase with sangivamycin. Jpn. J. Clin Oncol 37:867-873, 2007.

6. Komura, N., Asakawa, M., Umezawa, K. & Segawa, K.: Tyrosine kinase inhibitor methyl-2,5-dihydroxycinnamate induces PML nuclear body formation and apoptosis in tumor cells. Exp. Cell Res. 313: 2753-2765, 2007.

7. Kubota, T., Hoshino, M., Aoki, K., Ohya, K., Komano, Y., Nanki, T., Miyasaka, N. & Umezawa, K.: NF-κB inhibitorDHMEQ suppresses osteoclastogenesis and expression of NFATc1 in mouse arthritis without affecting expression of RANCL, OPG or M-CSF. Arthritis Research & Therapy 9/5/R97 Open Access, 2007.

8. Nagai. N, Oike, Y., Izumi-Nagai. K, Koto, T., Satofuka, S., Ozawa, Y., Yamashiro, K., Inoue, M., Tsubota, K., Umezawa, K. & Ishida, S.: Suppression of diabetes-induced retinal inflammation by blocking angiotensin II type 1 receptor or its downstream NF-κB pathway. Invest. Ohthal. Vis. Sci. 48: 4342-4350, 2007.

9. Ohsugi, T., Kumasaka, T., Okada, S., Ishida, T., Yamaguchi, K., Horie, & R., Watanabe, T. & Umezawa, K.: Dehydroxymethylepoxyquinomicin (DHMEQ) therapy reduces tumor formation in mice inoculated with Tax-deficient adult T-cell leukemia–derived cell lines. Cancer Letters 257: 206-215, 2007.

10. Suzuki, E., Umezawa, K. & Bonavida, B.: Rituximab inhibits the constitutively activated PI3K-Akt pathway in B-NHL cell lines: Involvement in chemosensitization to drug-induced apoptosis. Oncogene 26: 6184-6189, 2007.

11. A. Katsman, K. Umezawa and B. Bonavida: Reversal ofresistance to cytotoxic cancer therapies: DHMEQ as a chemo-sensitizing and immuno-sensitizing agent. Drug Resistance Updates 10: 1-12, 2007.

12. 梅澤一夫：炎症を抑制する生理活性物質の探索。蛋白質　核酸　酵素 　52 (No. 13): 1685-1689, 2007.

13. 鈴木絵里子、梅 澤 一 夫：NF-κBを標的とする抗癌剤。医学のあゆみ 、224: 65-69, 2008.

(2008 年度 )1. Baritaki, S., Suzuki, E., Umezawa, K., Spandidos, D. A., Berenson, J.,

Daniels, T. R., Penichet, M. L., Jazirehi, A. R., Palladino, M. Bonavida, M. Inhibition of Yin Yang 1-dependent repressor activity of DR5 transcription and expression by the novel proteasome inhibitor NPI-0052 contributes to its TRAIL-enhanced apoptosis in cancer cells. J. Immunol. 180, 6199-6210 (2008)

2. Bonavida, B., Jazirehi, A. R., Vega, M. I., Huerta-Yepez, S., Umezawa, K., Suzuki, E. Chemo-immunosensitization of resistant B-NHL as a result of rituximab (anti-CD20 mAb)-mediated inhibition of cell survival signaling pathways. in B. Bonavida (editor) Sensitization of Cancer Cells for Chemo/Immuno/Radio-therapy, pp29-40, Springer (2008)

3. Hisanaga, E., Park, K-Y., Yamada, S., Hashimoto, H., Takeuchi, T., Mori, M., Seno, M., Umezawa, K., Takei, I., Kojima, I., A simple method to induce differentiation of murine bone marrow mesenchymal cells to insulin-

producing cells using conophylline and betacellulin-delta4. Endocrine J. 55, 535-543 (2008)

4. Horiuchi, K., Morioka, H., Nishimoto, K., Susa, M., Nakayama, R., Kawai, A., Sonobe, H., Takaishi, H., Ozaki, T., Yabe, H., Umezawa, K., Toyama, Y. Growth suppression and apoptosis induction in synovial sarcoma cell lines by a novel NF-κB inhibitor, Dehydroxymethylepoxyquinomicin (DHMEQ). Cancer Letters 272, 336-344 (2008)

5. Ishikawa, Y., Tachibana, M., Matsui, C., Obata, R., Umezawa, K Nishiyama, S. on novel analogs of 9-methyl-streptimidone, an inhibitor of NF- κ B. Bioorg. Med. Chem. Lett. 19, 1726-1728 (2009)

6. Kosaka, T., Miyajima, A., Kikuchi, E., Horiguchi, Y., Umezawa, K., Ohigashi, T., Nakashima, J., Asano, T., Oya, M. The novel NF- κ B activation inhibitor dehydroxymethyl-epoxyquinomicin suppresses anti-Thy1.1-induced glomerulonephritis in rats. Nephron Exp. Nephrol. 110, e17–e24 (2008)

7. Makino, S., Mitsutake, N., Nakashima, M., Saenko, V. A., Ohtsuru, A., Umezawa, K., Tanaka, K., Hirano, A., Yamashita, S. DHMEQ, a novel NF-κB inhibitor, suppresses growth and type I collagen accumulation in keloid fibroblasts. J. Dermatol. Sci. 51, 171-180 (2008)

8. Vega, M. I., Martinez-Paniagua, M., Jazirehi, A. R., Huerta-Yepez, S., Umezawa, K., Martinez-Maza, O., Bonavida, B. The NF-κB inhibitors (bortezomib and DHMEQ) sensitise rituximab-resistant AIDS-B-non-Hodgkin lymphoma to apoptosis by various chemotherapeutic drugs. Leukemia and Lymphoma 49, 1982-1994 (2008)

9. Meng, Z., Mitsutake, N., Nakashima, M., Starenki, D., Matsuse, M., Takakura, S., Namba, H., Saenko, V., Umezawa, K., Ohtsuru, A., Yamashita, S. Dehydroxymethylepoxyquinomicin, a novel nuclear Factor-kappaB inhibitor, enhances antitumor activity of taxanes in anaplastic thyroid cancer cells. Endocrinology 149, 5357-5365 (2008)

10. Miyake, A., Dewan, Z., Ishida, T., Watanabe, M., Honda, M., Sata, T., Yamamoto, N., Umezawa, K., Watanabe, T., Horie, R. Induction of apoptosis in Epstein-Barr virus-infected B-lymphocytes by the NF- κ B inhibitor DHMEQ. Microbes and Infection 10, 748-756 (2008)

11. Nishioka, C., Ikezoe, T., Jing, Y., Umezawa, K., Yokoyama, A. DHMEQ, a novel nuclear factor-κB inhibitor, induces selective depletion of alloreactive or phytohaemagglutinin-stimulated peripheral blood mononuclear cells, decreases production of T helper type 1 cytokines, and blocks maturation of dendritic cells. Immunology 124, 198-205 (2008)

12. Saisho,Y., Hirose, H., Horimai, C., Miyashita, K., Takei, I., Umezawa, K., Itoh, H. Effects of DHMEQ, a novel nuclear factor- κ B inhibitor on beta cell dysfunction in INS cells. Endocrine J. 55, 433-438 (2008)

13. Suzuki, T., Yamashita, K., Jomen, W., Ueki, S., Aoyagi, T., Fukai, M., Furukawa, H., Umezawa, K., Ozaki, M., Todo, S. The novel NF-κB inhibitor inhibitor, dehydroxymethylepoxyquinomicin, aprevents local and remote organ injury following intestinal ischemia/reperfusion in rats. J. Surg. Res. 149, 69-75 (2008)

14. Tachibana, M., Matsui, C., Takeuchi, Y., Suzuki, E., Umezawa, K. Inhibition of NF-κB activation by penicillic acid and dihydropenicillic acid isolated from fungi. Heterocycles 76, 1561-1569 (2008)

15. Watanabe, E., Mochizuki, N., Ajima, H., Ohno, K., Shiino, M., Umezawa, K., Fukai, M., Ozaki, M., Furukawa, H., Todo, S., Kishino, S. A simple and reliable method for determining plasma concentration of dehydroxymethylepoxyquinomicin by high performance liquid chromatography with mass spectrometry. J. Chromatography B 871, 32-36 (2008)

16. Watanabe, M., Nakashima, M., Togano, T., Higashihara, M., Watanabe, T., Umezawa, K., Horie, R. Identification of the RelA domain responsible for action of a new NF-κB inhibitor DHMEQ. Biochem. Biophys. Res. Commun, 376: 310-314 (2008)

17. Yamamoto, M., Horie, R., Takeiri, M., Kozawa, I., Umezawa, K. Inactivation of nuclear factor kappa B components by covalent binding of (-)-dehydroxymethylepoxyquinomicin to specific cysteine residues. J. Med. Chem., 51, 5780-5788 (2008)

18. 梅 澤 一 夫　シグナル伝達阻害剤の単離、分子デザインと生物活性。上村大輔　監修　新規素材探索-医薬品リード化合物・食品素材を求めて -　シーエムシー出版、pp68-78 (2008)

19. 梅澤一夫　生化学の基礎知識とケミカルバイオロジー。長田裕之編　入門ケミカルバイオロジー　 オーム社　pp155-172 (2008)

20. 鈴木絵里子、梅澤一夫　NF-κBの役割と阻害剤の抗癌活性。鶴尾隆編　癌の分子標的　 南山堂 pp126-131 (2008)

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21. 鈴木絵里子、梅 澤 一 夫　NF-κBを標的とする抗癌剤。医学のあゆみ 244, 65-69 (2008)

22. 鈴木絵里子、梅 澤 一 夫　シグナル伝達因子とがん治療薬開発-NF-kappa B阻害剤(-)-DHMEQ-　バイオ医薬開発技術とシーズ 、シーエムシー出版、pp79-89 (2009)

(2009 年度 )1. E. Suzuki, C. Sugiyama and K. Umezawa: Inhibition of inflammatory

mediator secretion by (-)-DHMEQ in mouse bone marrow-derived macrophages. Biomed. Pharmacother. 63: 351-358, 2009.

2. Y. Ishikawa, M. Tachibana, C. Matsui, R. Obata, K. Umezawa and S. Nishiyama: Synthesis and biological evaluation on novel analogs of 9-methyl-streptimidone, an inhibitor of NF-κB. Bioorg. Med. Chem. Lett. 19: 1726-1728, 2009.

3. E. Suzuki, Y. Ninomiya and K. Umezawa: Induction of hisitidine decarboxylase in macrophages inhibited by the novel NF-kappa B inhibitor (-)-DHMEQ. Biochem. Biophys. Res. Commun. 379: 379-383, 2009.

4. E. Suzuki, H. Ogura, K. Kato, I. Takei, Y. Kabe, Hiroshi Handa and K. Umezawa: Preparation of conophylline affinity nanobeads and identification of a target protein. Bioorg. Med. Chem. 17: 6185-6195, 2009.

5. J. Inumaru, O. Nagano, E. Takahashi, T. Ishimoto, S. Nakamura, Y. Suzuki, S. Niwa, K. Umezawa, H. Tanihara and H. Saya: Molecular mechanisms regulating dissociation of cell-cell junction of epithelial cells by oxidative stress. Genes Cells 14:706-716, 2009.

6. N. Dabaghmanesh, A. Matsubara, A. Miyake, K. Nakano, T. Ishida, H. Katano, R. Horie, K. Umezawa and T. Watanabe: Transient inhibition of NF-κB by DHMEQ i nduces cell death of primary effusion lymphoma without HHV-8 reactivation. Cancer Science 100:737-746, 2009.

7. I. Kozawa, K. Kato, T. Teruya, K. Suenaga and K. Umezawa: Unusual intramolecular N → O acyl group migration occurring during conjugation of (-)-DHMEQ with cysteine. Bioorg. Med. Chem. Lett. 19: 5380-5382, 2009.

8. T. Saitoh, E. Suzuki, A. Takasugi, R. Obata, Y. Ishikawa, K. Umezawa and S. Nishiyama: Efficient synthesis of ( ± )-parasitenone, a novel inhibitor of NF-kB. Bioorg. Med. Chem. Lett. 19: 5383-5386, 2009.

9. K. Toshima, S. Tanimoto, K. Tsumura, K. Umezawa and D. Takahashi: Target-selective degradation of cancer-related proteins by novel photosensitizers for molecular-targeted photodynamic therapy. (Review article) Cancer Science 100: 1581-1584, 2009.

10. T. Kubota, Y. Fukuya, R. Hashimoto, T. Kanda, H. Suzuki, Y. Okamura, T. Nanki, N. Miyasaka and K. Umezawa: Possible involvement of chemokine-induced platelet activation in thrombophilic diathesis of antiphospholipid syndrome: an attractive target for the NF-κB-specific inhibitor DHMEQ. Ann. N. Y. Acad. Sci. 1173: 137-145, 2009.

11. N. Tsukamoto, S. Okada, Y. Onami, Y. Sasaki, K. Umezawa and Y. Kawakami: Impairment of plasmacytoid dendritic cells for IFN production by the ligand for immunoglobulin-like transcript 7 expressed on human cancer cells. Clin. Cancer Res. 15: 5733-5743, 2009.

12. M. Hamada, Y. Inami, Y. Nagai, T. Higashi, M. Shoji, S. Ogawa, K . U m e z a w a , T. S u g a i : E n z y m e - c a t a l y z e d re s o l u t i o n o f 3,8-dioxatricyclo[3.2.1.02,4]octane-6-carboxylic esters and the application to the synthesis of 3-epishikimic acid. Tetrahedron: Asymmetry 20: 2105-2111, 2009.

13. T. Togano, M. Sasaki, M. Watanabe, M. Nakashima, T. Tsuruo, K. Umezawa, M. Higashihara, T. Watanabe and R. Horie: Induction of oncogene addiction shift to NF-κB by camptothecin in solid tumor cells. Biochem. Biophys. Res. Commun. 390: 60-64, 2009.

14. M. Fujii, I. Takei and K. Umezawa: Antidiabetic effect of plant extract containing conophylline by oral administration in streptozotocin-treated and Goto-Kakizaki rats. Biomedicine &. Pharmacotherapy 63: 710-716, 2009.

15. N. Lampiasi, A. Azzolina, N. DʼAlessandro, K. Umezawa, J. A. McCubrey, G. Montalto and M. Cervello: Antitumor effects of DHMEQ, a novel NF-κB inhibitor, in human liver cancer cells is mediated through a reactive oxygen species-dependent mechanism. Molecular Pharmacology 76:290-300, 2009.

16. M. Morioka, A. Kamizono, H. Takikawa, A. Mori, H. Ueno, S. Kadowaki, Y. Nakao, K. Kato and K. Umezawa: Design, synthesis, and biological

evaluation of novel estradiol-bisphosphonate conjugates as bone-specific estrogens. Bioorg. Med. Chem. Bioorg. Med. Chem. 11: 1143-1148, 2010.

17. 鈴木絵里子、梅澤一夫：シグナル伝達因子とがん治療薬開発 -NF-kappa B 阻害剤 (-)-DHMEQ-　バイオ医薬開発技術とシーズ 、シーエムシー出版、pp79-89, 2009.

18. 鈴木絵里子、梅澤一夫：新しい抗癌剤の分子標的 NF-κB-。菅野純夫編 ：臨床遺伝子学 ʼ09- がんの遺伝子学 -　最新医学 9 月増刊号 、最新医学社、pp2141-2150, 2009.

19. 梅澤一夫：抗悪性腫瘍薬の作用機構解析法。曽根三郎、鶴尾隆編：がん分子標的治療研究実践マニュアル pp59-63, 2009

(2010 年度 )1. E. Suzuki, T. R. Daniels, G. Helguera, M. L. Penichet, K. Umezawa and B.

Bonavida: Inhibition of NF- κ B and Akt pathways by an antibody-avidin fusion protein sensitizes malignant B-cells to cisplatin-induced apoptosis. International J. Oncology 36:1299-1307, 2010.

2. M. Kawakami, A. Hirayama, K. Tsuchiya, H. Ohgawara, M. Nakamura, and K. Umezawa: Promotion of β-cell differentiation by the alkaloid conophylline in porcine pancreatic endocrine cells. Biomedicine & Pharmacotherapy 64: 226–231, 2010.

3. M. Takeuchi, S. Kimura, J. Kuroda, E. Ashihara, M. Kawatani, H. Osada, K. Umezawa, E. Yasui, M. Imoto, T. Tsuruo, A. Yokota, R. Tanaka, R. Nagao, T. Nakahata, Y. Fujiyama and T Maekawa: Glyoxalase-I is a novel target against Bcr-Abl+ leukemic cells acquiring stem-like characteristics in hypoxic environment. Cell Death Diff 2010: 1-10, 2010.

4. K. Kodaira, E. Kikuchi, M. Kosugi, Y. Horiguchi, K. Matsumoto, K. Kanai, E. Suzuki, A. Miyajima, K. Nakagawa, M. Tachibana, K. Umezawa and M. Oya: Potent cytotoxic effect of a novel nuclear factor-kappa B inhibitor on human bladder cancer cells producing various cytokines. Urology 75: 805-812, 2010.

5. M. Murohashi, K. Hinohara, M. Kuroda, T. Isagawa, S. Tsuji, S. Kobayashi, K. Umezawa, A. Tojo, H. Aburatani and N. Gotoh: Gene set enrichment analysis provides insight into novel signaling pathways in breast cancer stem cells. British J. Cancer 102: 206-212, 2010.

6. R. Dror, M. Lederman, K. Umezawa, V. Barak, J. Peʼer and I. Chowers: Characterizing the involvement of the nuclear factor-kappa B (NFκB) transcription factor in uveal melanoma. Investigative Ophthalmology & Visual Science 58: 1811-1816, 2010.

7. D. Iwata, N. Kitaichi, A. Ebihara, K. Iwabuchi, K. Yoshida, K. Namba, M. Ozaki, S. Ohno, K. Umezawa, K. Yamashita, S. Todo, S. Ishida and K. Onoé: Nuclear factor-κB inhibitor, dehydroxy methyl epoxyquinomicin ameliorates experimental autoimmune uveoreitnitis (EAU) in mice. Investigative Ophthalmology & Visual Science 51: 2077-2084, 2010.

8. C. Shimada, Y. Ninomiya, E. Suzuki and K. Umezawa: Efficient cellular uptake of the novel NF-κB inhibitor (-)-DHMEQ and irreversible inhibition of NF-κB in neoplastic cells. Oncology Research, 18: 529-535, 2010.

9. A. Miyake, T. Ishida, M. Yamagishi, T. Hara, K. Umezawa, T. Watanabe and R. Horie: Inhibition of active HIV-1 replication by NF- κ B inhibitor DHMEQ. Microbes and Infection 12: 400-408, 2010.

10. A. Hamasaka, N. Yoshioka, R. Abe, S. Kishino, K. Umezawa, M. Ozaki, S. Todo and H. Shimizu: Topical application of DHMEQ improves allergic inflammation via NF-κB inhibition. Journal of Allergy and Clinical Immunology 126: 400-403, 2010.

11. 11 M. Hamada, T. Higashi, M. Shoji, K. Umezawa and T. Sugai: Lipase-catalyzed enantio- and regioselective transformation of 3-epi-shikimic acid derivatives as the key step for the entry of polyoxygenated carbacycles. Journal of Molecular Catalysis B: Enzymic 67: 78-84, 2010.

12. M. Hamada, Y. Niitsu, C. Hiraoka, I. Kozawa, T. Higashi, M. Shoji, K. Umezawa, T. Sugai: Chemoenzymatic synthesis of (2S,3S,4S)-form, the physiologically active stereoisomer of dehydroxymethylepoxyquinomicin (DHMEQ), a potent inhibitor on NF-κB. Tetrahedron 66:7083-7087, 2010.

13. N. Miyanishi, Y. Suzuki, S. Simizu, Y. Kuwabara, K. Banno and K. Umezawa: Involvement of autocrine CXCL12/CXCR4 system in the regulation of ovarian carcinoma cell invasion. Biochem. Biophys. Res. Commun. 403: 154-159, 2010.

14. V. Cardile, G. Frasca, M. Libra, S. Caggia, K. Umezawa, A. Panico and G. Malaponte: Dehydroxyquinomicin inhibits expression and production of inflammatory mediators in interleukin-1 β-induced human chondrocytes.

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ents

Cellular Physiology and Biochemistry 25: 543-550, 2010.15. T. Takahashi, S. Matsumoto, M. Matsushita, H. Kamachi, Y. Tsuruga, H.

Kasai, M. Watanabe, M. Ozaki, H. Furukawa, K. Umezawa and S. Todo. Donor pretreatment with DHMEQ improves islet transplantation. Journal of Surgical Research 163: e23-34, 2010.

16. T. Saitoh, C. Shimada, M. Takeiri, M. Shiino, S. Ohba, R. Obata, Y. Ishikawa, K. Umezawa, S. Nishiyama: A novel NF-κB inhibitor based on the amino-epoxyquinol core of DHMEQ. Bioorg. Med. Chem. Lett. 20:5638-5642, 2010.

17. V. Cardile, M. Libra, S. Caggia, G. Frasca, K. Umezawa, F. Stivala, M. C. Mazzarino, Y. Bevelacqua, M. Coco and G. Malaponte: Dehydroxyquinomicin, a novel NF-kappaB inhibitor, prevents inflammatory injury induced by IFN-γ and histamine in keratinocytes NCTC 2544. Cellular and Experimental Pharmacology and Physiology 37: 679-683, 2010.

18. K. Shinoda, K. Nakagawa, T. Kosaka, N. Tanaka, T. Maeda, H. Kono, R. Mizuno, E. Kikuchi, A. Miyajima, K. Umezawa, M. Oya: Regulation of human dendritic cells by a novel specific nuclear factor-κB inhibitor, dehydroxymethylepoxyquinomicin. Human Immunology 71: 763-770, 2010.

19. L. Levati, F. Ruffini, A. Muzi, K. Umezawa, G. Graziani, S. DʼAtri, P. M. Lacal: Placenta growth factor induces melanoma resistance to temozolomide through a mechanism that involves the activation of the transcription factor NF- κ B. International J. Oncol. 38: 241-247, 2011.

（2011 年度）1. K. Mino, M. Ozaki, K. Nakanishi, S. Haga, M. Sato, M. Kina, M. Takahashi,

N. Takahashi, A. Kataoka, K. Yanagihara, T. Ochiya, T. Kamiyama, K. Umezawa and S. Todo: Inhibition of nuclear factor-kappaB suppresses peritoneal dissemination of gastric cancer by blocking cancer cell adhesion. Cancer Science 102: 1052-1058, 2011.

2. K. Kobayashi, K. Umezawa and M. Yasui: Apoptosis in mouse amniotic epithelium is induced by activated macrophages through the TNF receptor type 1/TNF pathway. Biology of Reproduction 84: 248-254, 2011.

3. Y. Niitsu, M. Hakamata, Y. Goto, T. Higashi, M. Shoji, T. Sugai and K. Umezawa:Chemoenzymat ic synthesis of (2R,3R,4R)-dehydroxymethylepoxyquinomicin (DHMEQ), a new activator of antioxidant transcription factor Nrf2. Org. Biomol. Chem. 9: 4635-4641, 2011.

4. H. Hosoi, N. Kawai, H. Hagiwara, T. Suzuki, A.Nakazaki, K. Takao, K. Umezawa and S. Kobayashi: Synthesis and determination of the relative structure of akaterpin, a potent inhibitor of PI-PLC. Tetrahedron Letters 52: 4961-4964, 2011.

5. M. Takeiri, M. Tachibana, A. Kaneda, A. Ito, Y. Ishikawa, S. Nishiyama, R. Goto, K. Yamashita, S. Shibasaki, G. Hirokata, M. Ozaki, S. Todo and K. Umezawa: Inhibition of macrophage activation and suppression of graft rejection by DTCM-glutarimide, a novel piperidine derived from the antibiotic 9-methylstreptimidone. Inflammation Research, 60: 879-888, 2011.

6. K. Tsumura, A. Suzuki, T. Tsuzuki, H. Kaneko, S. Matsumura, M. Imoto, K. Umezawa, D. Takahashi and K. Toshima: Molecular design, chemical synthesis, and biological evaluation of agents that selectively photo-degrade the transcription factor estrogen receptor-α. Org. Biomol. Chem. 9: 6357-6366, 2011.

7. T. Fukushima, M. Kawaguchi, K. Yorita, H. Tanaka,K. Umezawa, H. Kataoka: Antitumor effect of dehydroxymethylepoxyquinomicin (DHMEQ), a small molecule inhibitor of nuclear factor- κ B, in glioblastoma. Neuro-Oncology, 14: 19-28, 2012.

8. T. Funakoshi, K. Yamashita, N. Ichikawa, M. Fukai, T. Suzuki, R. Goto, T. Oura, N. Kobayashi, T. Katsurada, S. Ichihara, M. Ozaki, K. Umezawa, and Satoru Todo: A novel NF-κB inhibitor, dehydroxymethylepoxyquinomicin, ameliorates inflammatory colonic injury in mice. Journal of Crohnʼs and Colotis, in press.

9. 須貝 威、梅澤 一夫：新しい NF-κB 阻害剤 DHMEQ の生物有機化学と抗炎症・抗がん剤への展望。化学と生物 　49: 406-414, 2011

10. K. Umezawa: Possible role of peritoneal NF-κB in peripheral inflammation and cancer: Lessons from the inhibitor DHMEQ. Biomedicine & Pharmacotherapy 5: 252-259, 2011.

11. E. Suzuki, S. Yamashita and K. Umezawa: Role of NF-κB in thyroid cancer.

In A. Carpi and J. L. Mechanick eds. “Thyroid Cancer, from Emergent Biotechnologies to Clinical Practice Guidelines”, pp 319-327, 2011.

特許権等の知的財産権1. (+-)-DHMEQ の酵素光学分割法および (-)-DHMEQ の製造方法 200 ８年 3 月 5 日出願。特願 2008-055322。出願人：慶應義塾 発明者：（理・教授）梅澤一夫 （理・准教授）須貝威 （理・学生）平岡千尋 （学

外）小澤郁子2. 医薬組成物 (DTCM-glutarimide ほか ) 2008 年 6 月 25 日出願。　特願 2008-166290。出願人：慶應義塾 発明者：（理・教授）梅澤一夫 （理・助教）石川裕一（理・教授）西山繁 （理・

学生）橘みゆき3. Nrf2 活性化剤 ((+)-DHMEQ ほか ) 2010 年 10 月 13 日出願。　特願 2010-230930。出願人：慶應義塾

発明者：（理・教授）梅澤一夫 （理・学生）袴田雅俊（理・学生）後藤優子

野崎智義

(2007 年度 )1. Sato, D., Yamagata, W., Harada, S. & Nozaki, T. Kinetic characterization

of methionine gamma-lyases from the enteric protozoan parasite Entamoeba histolyt ica against physiological substrates and trifluoromethionine, a promising lead compound against amoebiasis. FEBS J. 275, 548-560, 2008.

(2008 年度 )1. Ebert, F., Bachmann, A., Nakada-Tsukui, K., Hennings, I., Drescher, B.,

Nozaki, T., Tannich, E., Bruchhaus, I. An Entamoeba cysteine peptidase specifically expressed during encystation. Parasitol. Int. 57, 521-524 (2008)

2. Ghosh, E., Ghosh, A., Ghosh, A. N., Nozaki, T., Ganguly, S. Oxidative stress-induced cell cycle blockage and a protease-independent programmed cell death in microaerophilic Giardia lamblia. Drug Design, Development and Therapy 3, 103-110 (2009)

3. Hussain, S., Ali, V., Jeelani, G., and Nozaki, T. Isoform-dependent feedback regulation of serine O-acetyltransferase isoenzymes involved in L-cysteine biosynthesis of Entamoeba histolytica. Mol. Biochem. Parasitol. 163, 39–47 (2008)

4. Picazarri, K., Nakada-Tsukui, K., Nozaki, T. Autophagy during proliferation and encystation in the protozoan parasite Entamoeba invadens. Inf. Immun. 76, 278-288 (2008)

5. Picazarri, K., Nakada-Tsukui, K., Sato, D., Nozaki, T. Analysis of autophagy in the enteric protozoan parasite Entamoeba. Methods Enzymol. 451, 359-371 (2008)

6. Sato, D., Karaki, T., Shimizu, A., Kamei, K., Harada, S., and Nozaki, T. Crystallization and preliminary X-ray analysis of L-methionine γ -lyase 1 from Entamoeba histolytica. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 64, 697-699 (2008)

7. Sato, D., Yamagata, W., Harada, S., Nozaki, T. Kinetic characterization of methionine gamma-lyases from the enteric protozoan parasite Entamoeba histolytica against physiological substrates and trifluoromethionine, a promising lead compound against amoebiasis. FEBS J. 275, 548-560 (2008)

8. Wong, E., Okhonin, V., Berezovski, M., Nozaki, T., Alexandrov, K., Krylov, S. “Inject-mix-react-separate-and-quantitate” method for High-throughput screening of enzyme Inhibitors. J. Am. Chem. Soc. 130, 11862-11863 (2008)

(2009 年度 )1. Sato D, Kobayashi S, Yasui H, Shibata N, Toru T, Yamamoto M, Tokoro G,

Ali V, Soga T, Takeuchi T, Suematsu M, Nozaki T. Cytotoxic effect of amide derivatives of trifluoromethionine against the enteric protozoan parasite Entamoeba histolytica. Int J Antimicrob Agents 35(1), 56-61, 2010

2. Sato, D. and Nozaki, T. (2009) Methionine gamma-lyase: the unique reaction mechanism, physiological roles, and therapeutic applications against infectious diseases and cancers. IUBMB Life, 61, 1019-1028.

3. Mi-ichi, F., Yousuf, M. A., Nakada-Tsukui, K., and Nozaki, T. (2009) Mitosomes in Entamoeba histolytica contain a sulfate activation pathway. Proc. Natl. Acad. Sci. USA., 106, 21731–21736. [Also

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highlighted in Research Highlights of Nature Chemical Biology Feburary Issue"METABOLISM: A location for sulfation"(6, 81, 2010); Reviewed in Faculty of 1000, F1000 Factor 9.0)]

4. Sato., D., Kobayashi, S., Yasui, H., Shibata, N., Toru, T., Yamamoto, M., Tokoro, G., Ali, V., Soga, T., Takeuchi, T., Suematsu, M., and Nozaki, T. (2010) Cytotoxic effect of amide derivatives of trifluoromethionine to the enteric protozoan parasite Entamoeba histolytica. Int. J. Antimicrob. Agents., 35, 56-61.

5. Maralikova, B., Ali, V., Nakada-Tsukui, K., Nozaki, T., van der Giezen, M., Henze, K., and Tovar, J. (2010) Bacterial-type oxygen detoxification and iron-sulphur cluster assembly in amoebal relict mitochondria. Cell. Microbiol. 12, 331-342.

6. Husain, A., Jeelani, G., Sato, D., Ali, V,, and Nozaki, T. (2010) Characterization of two isotypes of L-threonine dehydratase from Entamoeba histolytica. Mol. Biochem. Parasitol. 170, 100-104.

(2010 年度 )1. Yousuf, M. A., Mi- ichi, F. , Nakada-Tsukui, K., and Nozaki, T.

(2010) Localization and targeting of unusual pyridine nucleotide transhydrogenase in Entamoeba histolytica. Eukaryot. Cell 9, 926-933.

2. Jeelani, G., Husain, A., Sato, D., Ali, V., Suematsue, M., Soga, T., and Nozaki, T. (2010) Two Atypical L-cysteine-regulated NADPH-dependent oxidoreductases involved in redox maintenance, L-cystine reduction, and metronidazole activation in the enteric protozoon Entamoeba histolytica. J. Biol. Chem., 285, 26889-26899.

3. Husain, A., Sato, D., Jeelani, G., Suematsu, M., Soga, T., and Nozaki, T. (2010) Metabolome analysis revealed increase in S-methylcysteine and phosphatidylisopropanolamine synthesis upon L-cysteine deprivation in the anaerobic protozoan parasite Entamoeba histolytica. J. Biol. Chem. 285, 39160-39170.

(2011 年度 )1. Husain, A., Jeelani, G., Sato, D., and Nozaki, T. Global analysis of

gene expression in response to L-cysteine deprivation in the anaerobic protozoan parasite Entamoeba histolytica. BMC Genomics 12, 275, 2011.

2. Penuliar, G. M., Furukawa, A., Sato, D., and Nozaki, T. Mechanism of trifluoromethionine resistance in Entamoeba histolytica. J. Antimicrob. Chemother. 66, 2045-2052, 2011.

3. Mi-ichi, F., Makiuchi, T., Furukawa, A., Sato, D., Nozaki, T. Sulfate activation in mitosomes plays a crucial role in the proliferation of Entamoeba histolytica. PLoS Negl. Trop. Dis. 5, e1263, 2011.

4. Mishra, V., Ali, V., Nozaki, T., and Bhakuni, V. Biophysical characterization of Entamoeba histolytica phosphoserine aminotransferase (EhPSAT): role of cofactor and domains in stability and subunit assembly. Eur Biophys J. 40, 599-610, 2011.

5. Watanabe, K., Gatanaga, H., Cadiz, A.E., Tanuma, J., Nozaki, T., Oka, S. Amebiasis in HIV-1-Infected Japanese Men: Clinical Features and Response to Therapy. PLoS Negl. Trop. Dis. 5:e1318, 2011.

6. Penuliar, G. M., Furukawa, A., Nakada-Tsukui, K., Husain, A., Sato, D., and Nozaki, T. Transcriptional and functional analysis of trifluoromethionine resistance in Entamoeba histolytica. J. Antimicrob. Chemother. In press.

塩見美喜子

(2009 年度 )1. Miyoshi K, Miyoshi T, Hartig JV, Siomi H, and Siomi MC. Functional

Molecular mechanisms that funnel RNA precursors into endogenous small-interfering RNA and micro RNA biogenesis pathways in Drosophila. RNA 16: 506-515. 2010

(2010 年度 )1. Siomi MC, Sato K, Pezic C, Aravin AA. PIWI-interacting small RNAs: the

vanguard of genome defence. Nat. Rev. Mol. Cell. Biol. 2011. 12: 246-258.

（2011 年度）1. Nagao A, Sato K, Nishida KM, Siomi H and Siomi MC. Gender-specific

hierarchy in nuage localization of PIWI-interacting RNA factors in

Drosophila. Front. Gene. 2:55 2011.

曽我朋義

(2007 年度 )1. Hashimoto, M., Ishihama, Y., Tomita, M., Soga, T., “Microelectrospray

Interface with Coaxial Sheath Flow for High-Resolution capillary electrophoresis/mass spectrometry Separation” Rapid Commun. Mass Spectrom. 21, 3579-3584, 2007.

2. Monton, R. M. N., Tomita, M., Soga, T., Ishihama, Y., “Polymer Entrapment in Polymerized Silicate for Preparing Highly Stable Capillary Coatings for CE and CE-MS”, Anal. Chem. 79, 7838-7844, 2007.

3. 石井伸佳、曽我朋義、冨田勝：トランスクリプトーム、プロテオーム、メタボローム、フラクソームの統合による大腸菌の代謝解明 , 実験医学 Vol.26(1) pp.24-29, 用土社 , 2008.

4. 曽我朋義：概論 - メタボロ－ムが解き明かす生命のシステム , 実験医学 Vol.26(1) pp.1-6, 用土社 , 2008.

5. 谷内江（木下）綾子、末松誠、曽我朋義、冨田勝：代謝シミュレーションとメタボロ－ム解析による赤血球低酸素応答の予測と実証 , 実験医学 Vol.26(1) pp.7-12, 用土社 , 2008.

6. 曽我朋義、大橋由明、冨田勝：メタボロミクスの医学への応用 , 臨床遺伝学 ʻ07　最新医学 2007　9 月増刊号 pp.56-63 (2040-2047), 最新医学社 , 2007.

7. 末松　誠、大村光代、小野江理成、菱木貴子、曽我朋義：メタボロ－ム解析技術の医学、生物学への応用 , 実験医学増刊：解明が進むメタボロリックシンドローム　実験医学 　Vol. 25（15） （増刊）pp.154(2364)-161(2391), 用土社 , 2007.

(2008 年度 )1. Sato, S., Arita, M., Soga, T., Nishioka, T., Tomita, M., “Time-resolved

Metabolomics Reveals Metabolic Modulation in Rice Foliage”, BMC Systems Biology doi:10.1186/1752-0509-2-51, 2, 51, 2008.

2. Shintani, T., Iwabuchi, T., Soga, T., Kato,Y., Yamamoto, T., Takano, N., Hishiki, T., Ueno, Y., Ikeda, S., Sakurakawa, T., Ishikawa, K., Goda, N., Kitagawa, Y., Kajimura, M., Matsumoto, K., Suematsu, M., “Cystathionine β-synthase as a Carbon Monoxide-sensitive Regulator of Bile Excretion” Hepatology 49, 141-150, 2009.

3. Kuramori, C., Hase, Y., Hoshikawa, K., Watanabe, K., Nishi, T., Hishiki, T., Soga, T., Nashimoto, A., Kabe, Y., Yamaguchi, Y., Watanabe, H., Kataoka, K., Suematsu, M., Handa, H., “Mono-(2-ethylhexyl) phthalate Targets Glycogen Debranching Enzyme and Affects Glycogen Metabolism in Rat Testis”, Toxicol. Sci. 109, 143-151, 2009.

4. 曽我朋義：最新のメタボローム測定法の開発と生命科学への応用 ,「新しい地平をひらく分析手法の最前線」化学フロンティア pp.21-29, 化学同人 , 2009.

5. 曽我朋義：CE-MS によるメタボローム測定法と生命科学への応用 ,「メタボローム特集」臨床化学 Vol37(4) pp.341-346, 日本臨床化学会 , 2008.

6. 紙健次郎、冨塚江利子、北潔、冨田勝、曽我朋義、江角浩安：がんメタボロームによるエネルギー代謝の解析とがんの微小環境を標的とした治療薬の開発に向けて ,「メタボローム特集」臨床化学 Vol37(4) pp.361-367, 日本臨床化学会 , 2008.

7. 石井伸佳、曽我朋義、冨田勝：細菌のマルチオミクス（各種網羅的測定データ）解析の最先端、化学と生物 Vol46(4) pp.228-229, 学会出版センター , 2008.

(2009 年度 )1. Sugimoto, M., Hirayama, A., Ihiskawa, T., Robert, M., Baran, R., Uehara,

K., Kawai, K., Soga, T., Tomita, M., “Differential Metabolomics Software for Capillary Electrophoresis-Mass Spectrometry Data Analysis” Metabolomics 6, 27-41, 2010.

2. Sugimoto, M., Wong, D., Hirayama, A., Soga, T., Tomita, M., “Capillary Electrophoresis Mass Spectrometry-based Saliva Metabolomics Identified Oral, Breast and Pancreatic Cancer-Specific Profiles” Metabolomics 6, 78-95, 2010.

3. Sato, D., Kobayashi, S., Yasui, H., Shibata, N., Toru, T., Yamamoto, M., Tokoro, G., Ali, V., Soga, T., Takeuchi, T., Suematsu, M., Nozaki, T., “Cytotoxic Effect of Amide Derivatives of Trifluoromethionine against The Enteric Protozoan Parasite Entamoeba histolytica” Int. J. Antimicrob. Agents. 35, 56-61, 2010.

4. Sugimoto, M., Koseki, T., Hirayama, A., Abe, S., Sano, T., Tomita, M.,

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100

業績集

List of Achievem

ents

Soga, T., “Correlation between Sensory Evaluation Scores of Japanese Sake and Metabolome Profiles” J. Agric. Food Chem. 58, 374–383, 2010.

5. Kato, T., Niizuma, S., Inuzuka, Y., Kawashima, T., Okuda, J., Yamaki, Y., Iwanaga, Y., Narazaki, M., Matsuda, T., Soga, T., Kita, T., Kimura, T., Shioi, T., “Analysis of Metabolic Remodeling in Compensated Left Ventricular Hypertrophy and Heart Failure” Circ. Heart Fail., 3, 420-430, 2010.

6. Kato, Y., Kubo, Y., Iwata, D., Kato, S., Sudo, T., Sugiura, T., Kagaya, T., Wakayama, T., Hirayama, A., Sugimoto, M., Sugihara, K., Kaneko, S., Soga, T., Asano, M., Tomita, M., Matsui, T., Wada, M., Tsuji, A. “Gene knockout and metabolome analysis of carnitine/organic cation transporter OCTN1” Pharm. Res., 27, 832-840, 2010.

7. Sakuragawa, T., Hishiki, T., Ueno, Y., Ikeda, S., Soga, T., Yachie-Kinoshita, A., Kajimura, M., Suematsu, M., “Hypotaurine is an energy-saving hepatoprotective compound against ischemia-reperfusion injury of the rat liver” J. Clin. Biochem. Nutr., 46, 126-134, 2010.

8. Minami, Y., Kasukawa, T., Kakazu, Y., Iigo, M., Sugimoto, M., Ikeda, S., Yasui, A., van der Horst, G., Soga, T., Ueda, H., “Measurement of Internal Body Time by Blood Metabolomics” Proc. Natl. Acad. Sci. USA 106, 9890-9895, 2009.

9. Hirayama, A., Kami, K., Sugimoto, M., Sugawara, M., Toki, N., Onozuka, H., Kinoshita, T., Saito, N., Ochiai, A., Tomita, M., Esumi, H., Soga, T., “Quantitative Metabolome Profiling of Colon and Stomach Cancer Microenvironment by Capillary Electrophoresis Time-of-flight Mass Spectrometry” Cancer Res. 69, 4918-4925, 2009.

10. Soga, T., Igarashi, K., Itoh, C., Mizobuchi, K., Zimmermann, H., Tomita, M., “Metabolomic Profiling of Anionic Metabolites by Capillary Electrophoresis Mass Spectrometry” Anal. Chem. 81, 6165-6174, 2009.

11. Nakahigashi, K., Toya, Y., Ishii, N., Soga, T., Hasegawa, M., Watanabe, H., Takai, Y., Honma, M., Mori, H., Tomita, M., “Systematic phenome analysis of Escherichia coli multiple-knockout mutants reveals hidden reactions in central carbon metabolism” Mol. Syst. Biol. 5, 306, doi:10.1038/msb.2009.65

12. Saito, N., Robert, M., Kochi, H., Matsuo, G., Kakazu, Y., Soga, T., Tomita, M., “Metabolite profiling reveals YihU as a novel hydroxybutyrate dehydrogenase for alternative succinic semialdehyde metabolism in Escherichia coli” J. Biol. Chem. 284, 16442-16451, 2009.

13. Toyohara, T., Suzuki, T., Morimoto, R., Akiyama, Y., Souma, T., Shiwaku, H.O., Takeuchi, Y., Mishima, E., Abe, M., Tanemoto, M., Masuda, S., Kawano, H., Maemura, K., Nakayama, M., Sato, H., Mikkaichi, T., Yamaguchi, H., Fukui, S., Fukumoto, Y., Shimokawa, H., Inui, K., Tetasaki, T., Goto, J., Ito, S., Hishinuma, T., Rubera, I., Tauc, M., Fujii-Kuriyama, Y., Yabuuchi, H., Moriyama, Y., Soga, T., Abe, T., “SLCO4C1 Transporter Eliminates Uremic Toxins and Attenuates Hypertension and Renal Inflammation” J. Am. Soc. Nephrol. 20, 2546-2555, 2009.

14. Iuchi, Y., Okada, F., Takamiya, R., Kibe, N., Tsunoda, S., Nakajima, O., Toyoda, K., Nagae, R., Suematsu, M., Soga, T., Uchida, K., Fujii, J. “Rescue of anaemia and autoimmune responses in SOD1-deficient mice by transgenic expression of human SOD1 in erythrocytes” Biochem. J. 422, 313-320, 2009.

15. Tajima, T., Goda, N., Fujiki, N., Hishiki, T., Nishiyama, Y., Senoo-Matsuda, N., Shimazu, M., Soga, T., Yoshimura, Y., Johnson, R.S., Suematsu, M., “HIF-1 α is necessary to support gluconeogenesis during liver regeneration” Biochem. Bioph. Res. Co. 387, 789-794, 2009.

16. Nishino, T., Yachie-Kinoshita, A., Hirayama, A., Soga, T., Suematsu, M., Tomota, M., “In Silico Modeling and Metabolome Analysis of Long-stored Erythrocytes to Improve Blood Storage Methods” J. Biotechnol. 144, 212-223, 2009.

17. 平山明由、曽我朋義：がん組織のメタボローム解析 バイオインダストリーVol27, No.3, pp50-56, シーエムシー出版 , 2010.

18. 曽我朋義：メタボロミクス 医学のあゆみ　 Vol231, Nos.12,13, pp1143, 医歯薬出版株式会社 , 2009.

19. 平山明由、曽我朋義：ヒト癌組織のメタボローム解析　医学のあゆみVol231, Nos.12,13, pp1145-1149, 医歯薬出版株式会社 , 2009.

20. 紙健次郎、冨塚江利子、冨田勝、北潔、江角浩安、曽我朋義：メタボローム解析による癌微小環境のエネルギー代謝の解明 , 癌と微小環境　 実験医学増刊 Vol27(2) pp.215-221, 用土社 , 2009.

21. 曽我朋義：メタボローム測定装置の発明 , 発明 The Invention Vol106(11) pp.29-31, 発明協会 , 2009.

(2010 年度 )1. Iino, K., Sugimoto, M., Soga, T., Tomita, M., “Profiling of the Charged

Metabol i tes of Tradit ional Herbal Medicines Using Capi l lary Electrophoresis time-of-flight Mass Spectrometry” Metabolomics online DOI: 10.1007/s11306-011-0290-7,2011.

2. Fujiwara, K., Ishihama, Y., Nakahigashi, K., Soga, T., Taguchi, H., “A Systematic Survey of in vivo Obligate Chaperonin-dependent Substrates” EMBO J. 29, 1552-1564, 2010.

3. Kitagawa, M., Ikeda, S., Tashiro, E., Soga, T., Imoto, M., “Metabolomic Identification of the Target of the Filopodia Protrusion Inhibitor Glucopiericidin A” Chem. Biol. 17, 989-998, 2010.

4. Husain, A., Sato, D., Jeelani, G., Mi-ichi F., Ali V., Suematsu, M., Soga, T., Nozaki, T., “Metabolome Analysis Revealed Increase in S-methylcysteine and Phosphatidylisopropanolamine Synthesis upon L-cysteine Deprivation in The Anaerobic Protozoan Parasite Entamoeba Histolytica” J. Biol. Chem. 285, 39160-39170, 2010.

5. Jeelani, G., Husain, A., Sato, D., Ali, V., Suematsu, M., Soga, T., Nozaki, T., “Two Atypical L-cysteine-regulated NADPH-dependent Oxidoreductases Involved in Redox Maintenance, L-cystine and Iron Reduction, and Metronidazole Activation in the Enteric Protozoon Entamoeba Histolytica” J. Biol. Chem. 285, 26889-26899, 2010.

6. Saito, N., Ohashi, Y., Soga, T., Tomita, M., “Unveiling Cellular Biochemical Reactions via Metabolomics-driven Approaches” Curr. Opi. Microbiol. 13, 358-362, 2010.

7. Sugimoto, M., Hirayama, A., Robert, M., Abe, S., Soga, T., Tomita, M., “Prediction of metabolite identity from accurate mass, migration time prediction and isotopic pattern information in CE-TOFMS data” Electrophoresis 31, 1-8, 2010.

8. Toya, Y., Ishii, N., Nakahigashi, K., Hirasawa, T., Soga, T., Tomita, M., Shimizu, K., “13C-Metabolic Flux Analysis for Batch Culture of Escherichia coli and Its pyk and pgi Gene Knockout Mutants Based on Mass Isotopomer Distribution of Intracellular Metabolites”, Biotechnol. Prog. 26, 975-992, 2010.

9. Sugimoto, M., Goto, H., Otomo K., Ito, M., Onuma, H., Suzuki, A., Sugawara M., Abe, S., Tomita, M., Soga, T., “Metabolomic Profiles and Sensory Attributes of Edamame under Various Storage Duration and Temperature Conditions” J. Agric. Food Chem. 58, 8418-8425, 2010.

10. Horai, H., Arita, M., Kanaya, S., Nihei, Y., Ikeda, T., Suwa, K., Ojima, Y., Tanaka, K., Tanaka, S., Aoshima, K., Oda, Y., Kakazu, Y., Kusano, M., Tohge, T., Matsuda, F., Sawada, Y., Hirai, Y. M., Nakanishi, H., Ikeda, K., Akimoto, N., Maoka, T., Takahashi, H., Ara, T., Sakurai, N., Suzuki, H., Shibata, D., Neumann, S., Iida, T., Tanaka, K., Funatsu, K., Matsuura, F., Soga, T., Taguchi, R., Saito, K., Nishioka, T., “MassBank: A Public Repository for Sharing Mass Spectral Data for Life Sciences” J. Mass Spectro. 45, 703-714, 2010.

11. 曽我朋義：CE-MS 法によるメタボローム解析と医薬への応用 遺伝子医学 MOOK19 号 , pp66-70, メディカル　ドウ , 2011.

12. 平 山 明 由、 曽 我 朋 義：CE-MS メ タ ボ ロ ミ ク ス Hormone Frontier in Glynecology Vol18, No.1, pp19-25, メディカルビュー社 , 2011.

13. 平山明由、曽我朋義：メタボローム解析による癌代謝特性の包括的解明 細胞工学 Vol30, No.1, pp25-29, 学研メディカル秀潤社 , 2011.

14. 曽我朋義：CE-MS メタボロミクスの最新動向 ぶんせき Vol461, No.11, pp585-590, 日本分析化学会 , 2010.

　　　(2011 年度 ) 1. Soga, T., Sugimoto, M., Honma, M., Mori, M., Igarashi, K., Kashikura, K.,

Ikeda, S., Hirayama, A., Yamamoto, T., Yoshida, H., Otsuka, M., Tsuji, S., Yatomi, Y., Sakuragawa, T., Watanabe, H., Nihei, K., Saito, T., Kawata, S., Suzuki, H., Tomita, M., Suematsu, M., “Serum Metabolomics Reveals γ-glutamyl Dipeptides as Biomarkers for discrimination among Different Forms of Liver Disease” J. Hepatol., 55, 896-905, 2011.

2. Deng, L., Shoji, I., Ogawa, W., Kaneda, S., Soga, T., Jiang, D., Ide, Y., Hotta, H., “Hepatitis C Virus Infection Promotes Hepatic Gluconeogenesis through an NS5A-Mediated, FoxO1-Dependent Pathway” J. Virol., 85, 8556-8568, 2011.

3. Kato, T., Niizuma, S., Inuzuka, Y., Kawashima, T., Okuda, J., Tamai, Y., Iwanaga, Y., Soga, T., Kita, T., Kimura, T., Shioi, T., “Analysis of Liver Metabolism in a Rat Model of Heart Failure” Int. J. Cardiol. online doi:10.1016/j.ijcard.2011.07.056, 2011.

4. Gustavsson, C., Soga, T., Wahlström, E, Vesterlund, M, Azimi, A.,

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Norstedt, G., Tollet-Egnell, P., “Sex-dependent Hepatic Transcripts and Metabolites in the Development of Glucose Intolerance and Insulin Resistance in Zucker Diabetic Fatty Rats” J. Mol. Endcrinol. 47, 129-143, 2011.

5. Kimura, T., Takabatake, Y., Takahashi, A., Kaimori, J., Matsui, I., Namba, T., Kitamura, H., Niimura, F., Matsusaka, T., Soga, T., Rakugi, H., Isaka, Y., “Autophagy Protects the Proximal Tubule from Degeneration and Acute Ischemic Injury” J. Am. Soc. Nephrol. 22, 902-913, 2011.

6. Sugimoto, M., Sakagami, H., Yokote, Y., Onuma, H., Kaneko, M., Mori, M., Sakaguchi, Y., Soga, T., Tomita, M., “Non-targeted metabolite profiling in activated macrophage secretion ” Metabolomics online DOI: 10.1007/s11306-011-0353-9, 2011.

7. Ogawa, Y., Koike, N., Kurosawa. G., Soga, T., Tomita, M., Tei, H., “Positive Autoregulation Delays the Expression Phase of Mammalian Clock Gene Per2” PLoS ONE 6(4), e18663, 2011.

8. Suzuki, T., Toyohara, T., Akiyama, Y., Takeuchi, Y., Mishima, E., Suzuki, C., Ito, S., Soga, T., Abe, T., “Transcriptional regulation of Organic anion transporting polypeptide SLCO4C1 as a new therapeutic modality to prevent chronic kidney disease” J. Pharm. Sci. 100, 3696-3707, 2011.

特許権等の知的財産権（登録）（2008 年度、2009 年度）（登録）1. 発明の名称：シースフロー方式のキャピラリー電気泳動 - 質量分析計法に

よる陰イオン性化合物の測定装置発明者名：曽我朋義出願番号：特願 2009-056132出願日：2009 年 3 月 10 日登録日：2009 年 10 月 19 日特許番号：特許第 4385171 号

（出願中）2. 発明の名称：肝臓疾患マーカー、その測定方法、装置及び医薬品の検定方法発明者：曽我朋義、末松誠、本間雅、山本武人、鈴木洋史出願番号：優先特願 2008-328133　（特願 2010-543986）出願日：2008 年 12 月 24 日国際出願番号：PCT/JP2009/069950国際出願日：2009 年 11 年 26 日

（2010 年度）（出願中）1. 発明の名称：肝臓疾患マーカー、その測定方法、装置及び医薬品の検定方法発明者：杉本昌弘、斎藤貴史、河田純男、本間雅、鈴木洋史出願番号：特願 2010-102579出願日：2010 年 4 月 27 日

2. 発明の名称：肝臓疾患マーカー、その測定方法、装置及び医薬品の検定方法発明者：曽我朋義、杉本昌弘、斎藤貴史、河田純男出願番号：特願 2010-139736出願日：2010 年 6 月 18 日

冨田　勝

(2007 年度 )1. Arakawa, K. & Tomita, M. Selection effects on the positioning of genes

and gene structures from the interplay of replication and transcription in bacterial genomes. Evolutionary Bioinformatics 3:279-286(2007)

2. Arakawa, K. & Tomita, M. The GC skew index: a measure of genomic compositional asymmetry and the degree of replicational selection. Evolutionary Bioinformatics 3:145-154(2007)

3. Baran, R., Robert, M., Suematsu, M., Soga, T.& Tomita, M Visualization of three-way comparisons of omics data. BMC Bioinformatics, 8(1), 72, 2007

4. Fujishima, K., Komasa, M., Kitamura, S., Suzuki, H., Tomita, M. Kanai, A. Proteome-Wide Prediction of Novel DNA/RNA-Binding Proteins Using Amino Acid Composition and periodicity in the hyperthermophilic Archaeon Pyrococcus furiosus. DNA Research 14; 91-102(2007)

5. Fujishima, K., Sugahara, J., Tomita, M. & Kanai, A. Sequence Evidence

in the Archaeal Genomes that tRNAs emerged through the combination of ancestral genes as 5 ′ and 3 ′ tRNA halves. PLoS ONE journal 3(2):e1622(2008）

6. Hashimoto, M., Ishihama. Y., Tomita, M. & Soga, T. Microelectrospray interface with coaxial sheath flow for high-resolution capillary electrophoresis/mass spectrometry separation. Rapid Communications in Mass Spectrometry 21: 3579 - 3584(2007)

7. Imami, K., Sugiyama, N., Kyono, Y., Tomita, M., & Ishihama, Y. Automated Phosphoproteome Analysis for Cultured Cancer Cells by Two-Dimensional NanoLC-MS Using a Calcined Titania/C18 Biphasic Column. Innovations in Analytical Sciences 24; 161(2008)

8. Ishii, K., Nakamura, S., Morohashi, M., Sugimoto, M., Ohashi, Y., Kikuchi, S. & Tomita, M. Comparison of metabolite production capability indices generated by network analysis methods. Biosystems 91: 166-170(2007)

9. Ishii, N., Nakahigashi, K., Baba, T., Robert, M., Soga, T. , Kanai, A., Hirasawa, T., Naba, M., Hirai, K., Hoque, A., Yee, Ho Pei., Kakazu, Y., Sugawara, K., Igarashi, S., Harada, S., Masuda, T., Sugiyama, N., Togashi, T., Hasegawa, M., Takai, Y., Yugi, K., Arakawa, K., Iwata, N., Toya, Y., Nakayama, Y., Nishioka, T., Shimizu, K., Mori, H. & Tomita, M. Multiple high throughput analyses monitor the response of E. coli to perturbations. Science 316, 593-597 (2007)

10. Ishii, N., Nakayama, Y. & Tomita, M. Distinguishing of enzymes using metabolome data for the hybrid dynamic/static method. Theoretical Biology and Medical Modelling 4; 19(2007)

11. Itoh, H., Naito, Y. & Tomita, M. Simulation of developmental changes in action potential with ventricular cell models. Synthetic and Systems Biology Journal, 1:11-23(2007)

12. Kinoshita, A., Tsukada, K., Soga, T., Hishiki, T., Ueno, Y., Nakayama, Y., Tomita, M., Suematsu, M. Roles of hemoglobin allostery in hypoxia-induced metabolic alterations in erythrocytes: simulation and its verification by metabolome analysis. J. Biol. Chem. 282(14), 10731-10741, 2007.

13. Kinoshita, A., Nakayama, Y., Kitayama, T. & Tomita, M. Simulation study of methemoglobin reduction in erythrocytes. Differential contributions of two pathways to the tolerance of oxidative stress. FEBS Journal 274, 1449-1458 (2007)

14. Kochiwa, H., Tomita, M. & Kanai, A. Evolution of ribonuclease H genes in prokaryotes to avoid inheritance of redundant genes. BMC Evolutionary Biology 7; 128(2007)

15. Krishnan, A., Zbilut, JP., Tomita, M. & Giuliani, A. Proteins as Networks: Usefulness of Graph Theory in Protein Science. Currents in Protein and Peptide Science 9(1):28-38(2008）

16. Krishnan, A., Giuliani, A. & Tomita, M. Indeterminacy of Reverse Engineering of Gene Regulatory Networks: The Curse of Gene Elasticity. PLoS ONE 2; e562(2007)

17. Krishnan, A., Giuliani, A., Zbilut, JP. & Tomita, M. Network scaling invariants help to elucidate basic topological principles of proteins. Journal of Proteome Research 6; 3924-3934(2007)

18. Krishnan, A., Tomita, M. & Giuliani, A. Evolution of gene regulatory networks: Robustness as an emergent property of evolution. Physica A: Statistical Mechanics and its Applications 387; 2170-2186(2008)

19. Kuroki, A., Ohtani, N., Tsuge, K., Tomita, M. & Itaya, M. Conjugational transfer system to shuttle giant DNA cloned by Bacillus subtilis genome (BGM) vector. Gene 399; 72-80(2007)

20. Kuroki, A., Toda, T., Matsui, K., Uotsu-Tomita, R., Itaya M. & Tomita, M. Reshuffling of the Bacillus subtilis 168 genome by multifold inversion. Journal of Biochemistry 143(1):97-105(2008)

21. Matsubara, Y., Kikuchi, S., Sugimoto, M., Oka, K. & Tomita, M. Algebraic method for the analysis of signaling crosstalk. Artif Life 14; 81-94(2008)

22. Matsui, M., Yachie, N., Okada, Y., Saito, R. & Tomita, M. Bioinformatic analysis of post-transcriptional regulation by uORFs in human and mouse. FEBS Letters 581; 4184-4188(2007)

23. Matsuzaki, Y., Kikuchi, S. & Tomita, M. Robust effects of Tsr-CheBp and CheA-CheYp affinity in bacterial chemotaxis. Artificial Intelligence in Medicine 41: 145-150(2007)

24. Monton, M R., Tomita, M., Soga,, T. & Ishihama, Y. Polymer entrapment in polymerized silicate for preparing highly stable capillary coatings for CE and CE-MS. Journal of Analytical Chemistry 79:7838-7844(2007)

25. Mori, K., Saito, R., Kikuchi, S. & Tomita, M. Inferring rules of Escherichia coli translational efficiency using an artificial neural network. BioSystems

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90; 414-420 (2007) 26. Morohashi, M., Ohashi, Y., Tani, S., Ishii, K., Itaya, M., Nanamiya, H.,

Kawamura, F., Tomita, M. & Soga, T. Model based definition of population heterogeneity and its effects on metabolism in sporulating Bacillus subtilis. Journal of Biochemistry 142:183-191 (2007)

27. Morohashi, M., Shimizu, K., Ohashi, Y., Abe, J., Mori, H., Tomita, M. & Soga, T. P-BOSS: A new filtering method for treasure hunting in metabolomics. Journal of Chromatography A 1159; 142–148 (2007)

28. Ogawa, Y., Arakawa, K., Kaizu, K., Miyoshi, F., Nakayama, Y. & Tomita, M. Comparative Study of Circadian Oscillatory Network Models of Drosophila. Artif Life 14; 29-48(2008)

29. Ohashi, Y., Hirayama, A., Ishikawa, T., Nakamura, S., Shimizu, K., Ueno, Y., Tomita, M. & Soga, T. Depiction of metabolome changes in histidine-starved Escherichia coli by CE-TOFMS. Mol. BioSyst 4; 135-147(2008)

30. Ohno, H., Naito, Y. & Nakajima, H. & Tomita, M. Construction of a Biological Tissue Model Based on a Single-Cell Model: A Computer Simulation of Metabolic Heterogeneity in the Liver Lobule. Artif Life 14; 3-28(2008)

31. Oya, Y., Ishii, N., Hirasawa, T., Tomita, M. & Soga, T. Direct measurement of isotopomer of intracellular metabolites using capillary electrophoresis time-of-flight mass spectrometry for efficient metabolic flux analysis. Journal of Chromatography A 1159; 134–141 (2007)

32. Piras, V., Selvarajoo, K., Fujikawa, N., Choi, S., Tomita, M., Giuliani, A. & Tsuchiya, M. Statistical Analysis of Gene Expression in Innate Immune Responses: Dynamic Interactions between MicroRNA and Signaling Molecules. Genomics and Informatics 5; 107-112 (2007)

33. Shinoda, K., Sugimoto, M., Tomita M. & Ishihama, Y. Informatics for Peptide Retention Properties in Proteomic LC-MS. Proteomics 8(4):787-98(2008)

34. Soga, T., Ishikawa, T., Igarashi, S., Sugawara, K., Kakazu, Y. & Tomita, M. Analysis of nucleotides by pressure-assisted capillary electrophoresis mass spectrometry using si lanol mask technique. Journal of Chromatography A 1159; 125–133(2007)

35. Soma, A., Onodera, A., Sugahara, J., Kanai, A., Yachie, N., Tomita, M., Kawamura, F. & Sekine, Y. Permuted tRNA Genes Expressed via a Circular RNA Intermediate in　Cyanidioschyzon merolae. Science 318:450-453(2007)

36. Sugiyama, N., Masuda, T., Shinoda, K., Nakamura, A., Tomita, M. & Ishihama, Y. Phosphopeptide Enrichment by Aliphatic Hydroxy Acid-modified Metal Oxide Chromatography for Nano-LC-MS/MS in Proteomics Applications. Mol Cell Proteomics 6; 1103-1109(2007)

37. Tamaki, S., Arakawa, K., Kono, N. & Tomita, M. Restauro-G: A Rapid Genome Re-Annotation System for Comparative Genomics. Genomics Proteomics Bioinformatics 5; 53-58(2007)

38. Toya, Y., Ishii, N., Hirasawa, T., Naba, M., Hirai, K., Sugawara, K., Igarashi, S., Shimizu, K., Tomita, M. & *Soga, T. “Direct Measurement of The Isotope Distribution of Intracellular Metabolites Using CE-TOFMS for Efficient Metabolic Flux Analysis”, J. Chromatogr. A 1159, 134-141, 2007.

39. Tsuchyia, M., Wong, ST., Yeo, ZX., Colosimo, A., Palumbo, MC., Farina, L., Crescenzi, M., Mazzola, A., Negri, R., Bianchi, MM., Selvarajoo, K., Tomita, M. & Giuliani, A. Gene expression waves: Cell cycle independent collective dynamics in cultured cells. FEBS Journal 274; 2878-2886 (2007)

40. Watanabe, Y., Kishi, A., Yachie, N., Kanai, A. & Tomita, M. Computational analysis of microRNA-mediated antiviral defense in humans. FEBS Letters 581; 4603-4610(2007)

41. Watanabe, Y., Tomita, M. & Kanai, A. Computational Methods for MicroRNA Target Prediction. Methods in Enzymology 427; 65-86(2007)

(2008 年度 )1. Arakawa, K., Tamaki, S., Kono, N., Kido, N., Ikegami, K., Ogawa, R.,

Tomita, M. Genome Projector: zoomable genome map with multiple views. BMC Bioinformatics 10:31 (2009)

2. Arakawa, K., Yachie, N., Tomita, M. Visualizing Complex Omics Information-Scientific Visualization for Genomics and Systems Biology. BIOforum Europe6:27-29 (2008)

3. Fujishima, K., Sugahara, J., Kikuta, K. Hirano, R.,Sato, A., Tomita, M. and Kanai, A. Tri-split tRNA: A transfer RNA made from three transcripts provides insight into the evolution of fragmented tRNAs in archaea. Proc Natl Acad Sci U S.A. 106, 2683-2687(2009)

4. Helmy, M., Gohda, J, Inoue, J., Tomita, M. Tsuchiya, M. Predicting novel features of toll-like receptor 3 signaling in macrophages.. Selvarajoo, K. PLoS ONE. 4:e4661(2009)

5. Imami, K., Sugiyama, N., Kyono, Y., Tomita, M., Ishihama, Y. Automated Phosphoproteome Analysis for Cultured Cancer Cells by Two-Dimensional NanoLC-MS Using a Calcined Titania/C18 Biphasic Column. Innovations in Analytical Sciences 24: 161(2008)

6. Kosugi, S., Hasebe, M., Entani, T., Takayama, S., Tomita, M., Yanagawa, H.: Design of peptide inhibitors for importin α / β nuclear import pathway by activity-based profiling. Chem.& Biol., 15, 940-949 (2008)

7. Kosugi, S., Hasebe,M.,Tomita, M., Yanagawa, H,: Nuclear export signal consensus sequences defined using a localization-based yeast selection system. Traffic,9, 2053-2062 (2008)

8. Kyono, Y.,Sugiyama, N., Imami, K., Tomita, M., Ishihama, Y. Successive and Selective Release of Phosphorylated Peptides Captured by hydroxy Acid-Modified Metal Oxide Chromatography. Journal of Proteome Research 7:4585-4593 (2008)

9. Matsubara, Y., Kikuchi, S., Sugimoto, M., Oka, K., Tomita, M. Algebraic Method for the Analysis of Signaling Crosstalk. Artif Life 14; 81-94 (2008)

10. Ogawa, Y., Arakawa, K., Kaizu, K., Miyoshi, F., Nakayama, Y., Tomita, M. Comparative Study of Circadian Oscillatory Network Models of Drosophila. Artif Life 14; 29-48 (2008)

11. Ohashi, Y., Hirayama, A., Ishikawa, T., Nakamura, S., Shimizu, K., Ueno, Y., Tomita, M., Soga, T. Depiction of metabolome changes in histidine-starved Escherichia coli by CE-TOFMS. Mol. BioSyst 4: 135-147 (2008)

12. Ohno, H., Naito, Y., Nakajima, H., Tomita, M. Construction of a Biological Tissue Model Based on a Single-Cell Model: A Computer Simulation of Metabolic Heterogeneity in the Liver Lobule. Artif Life 14; 3-28 (2008)

13. Ooga, T., Ohashi, Y., Kuramitsu, S., Koyama, Y., Tomita, M., Soga, T., Masui, R. Degradation of ppGpp by nudix pyrophosphatase modulates the transition of growth phase in the bacterium thermos thermophilus. J. Biol. Chem. doi:10.1074/jbc. M900582200 (2009)

14. Saito, N., Robert, M., Kochi, H., Matsuo, G., Kakazu, Y., Soga, T., Tomita, M. Metabolite profiling reveals YihU as a novel hydroxybutyrate dehydrogenase for alternative succinic semialdehyde metabolism in Escherichia coli. J. Biol. Chem. doi:10.1074/jbc. M109.002089, (2009)

15. Sato, S., Arita, M., Soga, T., Nishioka, T., Tomita, M. Time-resolved metabolomics reveals metabolic modulation in rice foliage. BMC Systems Biology 2:51 (2008)

16. Selvarajoo, K., Takabe, Y., Gohda, J., Helmy, M., Akira, S., Tomita, M., Tsuchiya, M., Inoue, J. Matsuo, K. Signaling Flux Redistribution at Toll-like Recepter Pathway Junctions. Plos one 3:e3430 (2008)

17. Shinoda, K., Tomita,M., Ishihama, Y. Aligning LC Peaks by Converting Gradient Retention Times to Retention Index of Peptides in Proteomic Experiments. Bioinformatics. 24: 1590-5 (2008)

18. Sugiyama, N., Nakagami, H., Mochida, K., Daudi, A., Tomita, M., Shirasu, K., Ishihama, Y. Large-scale phosphorylation mapping reveals the extent of tyrosine phosphorylation in Arabidopsis. Mol Syst Biol. 4:193 (2008)

19. Kyono,Y.,Sugiyama, N., Imami, K., Tomita, M. and Ishihama, Y. Successive and Selective Release of Phosphorylated Peptides Captured by hydroxy Acid-Modified Metal Oxide Chromatography. Journal of Proteome Research 7:4585-4593(2008)

(2009 年度 )1. Hirayama, A., Kami, K., Sugimoto, M., Sugawara, M., Toki, N., Onozuka,

H., Kinoshita, T., Saito, N., Ochiai, A., Tomita, M., Esumi, H., Soga, T.,”Quantitative Metabolome Profiling of Colon and Stomach Cancer Microenvironment by Capillary Electrophoresis Time-of-flight Mass Spectrometry” Cancer Res. 69, 4918-4925, 2009.

2. Soga, T., Igarashi, K., Itoh, C., Mizobuchi, K., Zimmermann, H., Tomita, M.,”Metabolomic Profiling of Anionic Metabolites by Capillary Electrophoresis Mass Spectrometry” Anal. Chem. 81, 6165-6174, 2009.

3. Nakahigashi, K., Toya, Y., Ishii, N., Soga, T., Hasegawa, M., Watanabe, H., Takai, Y., Honma, M., Mori, H., Tomita, M., “Systematic phenome analysis of Escherichia coli multiple knockout mutants reveals hidden reactions in central carbon metabolism” Mol. Syst. Biol., 5, 306, doi:10.1038/msb.2009.65

4. Saito, N., Robert, M., Kochi, H., Matsuo, G., Kakazu, Y., Soga, T., Tomita, M., “Metabolite profiling reveals YihU as a novel hydroxybutyrate dehydrogenase for alternative succinic semialdehyde metabolism in

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Escherichia coli” J. Biol. Chem. 109, 16442-16451, 20095. “Towards the systematic discovery of signal transduction networks using

phosphorylation dynamics data” Imamura, H., Yachie, N., Saito, R., Ishihama, Y. and Tomita, M. BMC Bioinformatics, (2010)

6. “Core promoter structure and genomic context reflect histone 3 lysine 9 acetylation patterns” Kratz, A., Arner, E., Saito, R., Kubosaki, A., Kawai, J., Suzuki, H., Carninci, P., Arakawa, T., Tomita, M., Hayashizaki, Y. and Daub, C.O. BMC Genomics (2010)

7. “Computational prediction of nucleosome positioning by calculating the relative fragment frequency index of nucleosomal sequences” Ogawa, R., Kitagawa, N., Ashida, H., Saito, R. and Tomita, M. FEBS Letters 584(8):1498-502.（2010）

8. “A new multicompartmental reaction-diffusion modeling method links transient membrane attachment of E. coli MinE to E-ring formation” Arjunan, S. N. V. and Tomita, M. Systems and Synthetic Biology journal (2009) 4:35-53 (2010)

9. “Phylogenetic position of a rare loricated green alga, Cephalomonas granulata N. L. Higinb. (Volvocales, Chlorophyceae)” Nakada, T., Soga, T. and Tomita, M. Phycological Research 58: 62- 68(2010)

10. “Quantitative analysis of replication-related mutation and selection pressures in bacterial chromosomes and plasmids using generalised GC skew index” Arakawa, K., Suzuki, H. and Tomita, M. BMC Genomics 10:640 (2009.12)

11. “Recharacterization of Chlamydomonas reinhardtii and its relatives with new isolates from Japan” Nakada, T., Shinkawa, H., Ito, T., and Tomita, M. J Plant Res 123: 67–78 (2009)

12. “Differential Metabolomics Software for Capillary Electrophoresis-Mass Spectrometry Data Analysis” Sugimoto, M., Hirayama, A., Ishikawa, T., Baran, R., Robert, M., Uehara, K., Soga, T. and Tomita, M., Metabolomics [Epub ahead of print] doi:10.1007/s11306-009-0175-1 (2009)

13. “Capillary electrophoresis mass spectrometry-based saliva metabolomics identified oral, breast and pancreatic cancer-specific profiles” Sugimoto, M., Wong, D. T., Hirayama, A., Soga, T. and Tomita, M. Metabolomics 6:78-95. (2009)

14. “Pathway Projector: Web-based Zoomable Pathway Browser using KEGG Atlas and Google Maps API” Kono, N., Arakawa, K., Ogawa, R., Kido, N., Oshita, K., Ikegami, K., Tamaki, S. and Tomita, M. PLoS ONE 4: e7710. (2009)

15. “In silico modeling and metabolome analysis of long-stored erythrocytes to improve blood storage methods” Nishino, T., Yachie, A. K., Hirayama. A., Soga, T., Suematsu, T., and Tomita, M. Journal of Biotechnology 144:212-223 (2009)

16. “Modeling reaction-diffusion of molecules on surface and in volume spaces with the E-Cell System” Arjunan, S. N. V., and Tomita, M. International Journal of Computer Science and Information Security. 3:211-216 (2009)

17. “A web server for interactive and zoomable Chaos Game Representation images” Arakawa, K., Oshita, K. and Tomita, M. Source Code for Biology and Medicine 4:6 (2009)

18. Hasegawa, M., Watanabe, H., Takai, Y., Honma, M., Mori, H. and Tomita, M., Molecular Systems Biology 5:306 ( 2009)

19. “ Sys tema t i c i den t i f i ca t i on o f ce l l cyc le -dependen t yeas t nucleocytoplasmic shuttling proteins by prediction of composite motifs” Kosugi, S., Hasebe, M., Tomita, M. and Yanagawa, H. Proc Natl Acad Sci U S A. 106:10171-6. (2009)

20. “Metabolomic Profiling of Anionic Metabolites by Capillary Electrophoresis Mass Spectrometry” Soga, T., Igarashi, K., Itoh, C., Mizobuchi, K., Zimmermann, H. P. and Tomita, M. Analytical Chemistry 81:6165-6174 (2009)

21. “Measure of synonymous codon usage diversity among genes in bacteria” Suzuki, H., Saito, R. and Tomita, M. BMC Bioinformatics 10:167. (2009)

22. "Cooperativity of two active sites in bacterial homodimeric aconitases " Tsuchiya, D., Shimizu, N. and Tomita, M. Biochem Biophys Res Commun　379:485-488(2009)

23. “Metabolite profi l ing reveals YihU as a novel hydroxybutyrate dehydrogenase for alternative succinic semialdehyde mtabolism in Eschericha coli” Saito, N., Robert, M., Kochi, H., Matsuo, G., Kakazu, Y., Soga, T. and Tomita, M. J. Biol. Chem. 284:16442-51 (2009)

24. Nishino T, Yachie-Kinoshita A, Hirayama A, Soga T, Suematsu M, Tomita M.

In silico modeling and metabolome analysis of long-stored erythrocytes to improve blood storage methods. J Bioengineer 144(3), 212-223, 2009.

25. Kosugi, S., Hasebe,M.,Tomita, M.,Yanagawa, H.: Systematic identification of cell cycle-dependent nucleocytoplasmic shuttling proteins by prediction of composite motifs, Pro.Natl.Acad.Sci.U.S.A., 106, 10171-10176 (2009)

26. Miyamoto-Sato, E., Ishizaka, M., Fujimori, S., Hirai, N., Masuoka, K., Saito, R., Ozawa, Y., Hino, K., Washio, T., Tomita, M., Yamashita, T., Oshikubo, T., Akasaka, H., Sugiyama, J., Matsumoto, Y., Yanagawa, H., A comprehensive resource of interacting protein regions for refining human transcription factor networks: Domain-based interactome. PLoS ONE, 5, e9289 (2010)

(2010 年度 )1. Yachie, A. K., Nishino, T., Shimo, H., Suematsu, M., and Tomita, M. “A

Metabolic Model of Human Erythrocytes: Practical Application of the E-Cell Simulation Environment” J Biomed Biotechnol 2010: 642420 (2010)

2. Sugimoto, M., Hirayama, A., Robert, M., Abe, S., Soga, T. and Tomita, M. “Prediction of metabolite identity from accurate mass, migration time prediction and isotopic pattern information in CE-TOFMS data” Electrophoresis 31:2311–2318 (2010)

3. Sugimoto, M., Hirayama, A., Ishikawa, T., Baran, R., Robert, M., Uehara, K., Soga, T. and Tomita, M. “Differential Metabolomics Software for Capillary Electrophoresis-Mass Spectrometry Data Analysis” Metabolomics 6:27-41(2010)

4. Murray, B.D., Haynes, K. and Tomita, M. “Redox Regulation in Respiring Saccharomyces cerevisiae” BBA - General Subjects, in press

5. Ooga, T., Sato, H., Nagashima, A., Sasaki, K., Tomita, M., Soga, T. and Ohashi, Y. “The Hyperlipidemia-drived metabolic imbalances revealed advanced oxidativestress in liver and its relief after simvastatin treatment” Molecular Biosystems. in press

6. Iino¸ K., Sugimoto¸ M., Soga¸ T. and Tomita, M. “Profiling of the Charged Metabolites of Traditional Herbal Medicines Using Capillary Electrophoresis Time-of-Flight Mass Spectrometry” Metabolomics DOI: 10.1007/s11306-011-0290-7 (2011)

7. Nozaki, T., Yachie, N., Ogawa, R., Saito, R. and Tomita, M. “Computational analysis suggests a highly bendable, fragile structure for nucleosomal DNA” Gene doi:10.1016/j.gene.2011.02.004 (2011)

8. Soga, T., Sugimoto, M., Honma, M., Mori, M., Igarashi, K., Kashikura, K., Ikeda, S., Hirayama, A., Yamamoto, T., Yoshida, H., Otsuka, M., Tsuji, S., Yatomi, Y., Sakuragawa, T., Watanabe, H., Nihei, K., Saito, T., Kawata, S., Suzuki, H., Tomita, M. and Suematsu, M. “Serum Metabolomics Reveals g-Gutamyl dipeptides as Biomarkers for Discrimination among Different Forms of Liver Disease” J. Hepatology doi:10.1016/j.jhep.2011.01.031 (2011)

9. Ooga, T., Sato, H., Nagashima, A., Sasaki, K., Tomita, M., Soga T. and Ohashi, Y. “Metabolomic Anatomy of Animal Model Revealing Homeostatic Imbalances in Dyslipidemia” Mol. BioSyst. 7:1217–1223 (2011)

10. Yachie, N., Saito, R., Sugiyama, N., Tomita, M. and Ishihama, Y. “Integrative features of the yeast phosphoproteome and protein-protein interaction map” PLoS Comput Biol. 7:e1001064(2011)

11. Kono, N., Arakawa, K. and Tomita, M. “Comprehensive prediction of chromosome dimer resolution sites in bacterial genomes” BMC Genomics 12:19 (2011)

12. Yoshida, S., Imoto, J., Minato, T., Oouchi, R., Kamada, Y., Tomita, M., Soga, T. and Yoshimoto, H. “A novel mechanism regulates H2S and SO2 production in Saccharomyces cerevisiae” Yeast 2011 28: 109–121 (2011)

13. Fujishima, K., Sugahara, J., Tomita, M. and Kanai, A. “Large-scale tRNA intron transposition in the archaeal order Thermoproteales represents a novel mechanism of intron gain” Mol. Biol. Evol. 27 : 2233–2243 (2010)

14. Tsuchiya, M., Piras, V., Giuliani, A., Tomita, M. and Selvarajoo, K. “Collective Dynamics of Specific Gene Ensembles Crucial for Neutrophil Differentiation: The Existence of Genome Vehicles Revealed” PLoS One 5: e12116 (2010)

15. Arakawa, K., Kido, N., Oshita, K. and Tomita, M. “G-language genome analysis environment with REST and SOAP web service interfaces” Nucleic Acids Research 38:W700–W705（2010）

16. Sugimoto, M., Goto, H., Otomo, K., Ito, M., Onuma, H., Suzuki, A., Sugawara, M., Abe, S., Tomita, M. and Soga, T. “Metabolomic Profiles

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and Sensory Attributes of Edamame under Various Storage Duration and Temperature Conditions.” J. Agric Food Chem. 58:8418–8425(2010)

17. Ozawa, Y., Saito, R., Fujimori, S., Kashima, H., Ishizaka, Hiroshi Yanagawa, H., Miyamoto-Sato, E. and Tomita, M. “Protein complex prediction via verifying and reconstructing the topology of domain-domain interactions” BMC Bioinformatics 11:350 (2010)

18. Nakada, T., Nozaki, H. and Tomita, M. “Another Origin of Coloniality in Volvocaleans: The Phylogenetic Position of Pyrobotrys Arnoldi (Spondylomoraceae, Volvocales)” J Eukaryot Microbiol 57:379-382 (2010)

19. Toya, Y., Ishii, N., Nakahigashi, K., Hirasawa, T., Tomoyoshi Soga,T., Tomita, M. and Shimizu, K. “13C-Metabolic flux analysis for batch culture of Escherichia coli and its pyk and pgi gene knockout mutants based on mass isotopomer distribution of intracellular metabolites” Biotechnology Progress 26:975-992(2010)

20.Saito, N., Ohashi, Y., Soga, T. and Tomita, M. “Unveiling cellular biochemical reactions via metabolomics-driven approaches” Current Opinion in Microbiology 13:358-362 (2010)

21. Kim, H., Lee, B. S., Tomita, M. and Kanai, A. “Transcription-Associated Mutagenesis Increases Protein Sequence Diversity More Effectively than Does Random Mutagenesis in Escherichia coli” PLoS ONE 5: e10567 (2010)

22. Imamura, H., Yachie, N., Saito, R., Ishihama, Y. and Tomita, M. “Towards the systematic discovery of signal transduction networks using phosphorylation dynamics data” BMC Bioinformatics, 11:232 (2010)

23. Iwasaki, M., Miwa, S., Ikegami, T., Tomita, M., Tanaka, N. and Ishihama, Y. “One-Dimensional Capillary Liquid Chromatographic Separation Coupled with Tandem Mass Spectrometry Unveils the Escherichia coli Proteome on a Microarray Scale” Analytical Chemistry 82: 2616-2620 (2010)

24. Ogawa, R., Kitagawa, N., Ashida, H., Saito, R. and Tomita, M. “Computational prediction of nucleosome positioning by calculating the relative fragment frequency index of nucleosomal sequences” FEBS Letters 584:1498-502（2010）

（2011 年度）In press1. “A GTP-dependent RNA 3'-terminal phosphate cyclase from the

hyperthermophilic archaeon Pyrococcus furiosus.”　Sato, A., Soga, T., Igarashi, K., Takesue, K., Tomita, M. and Kanai, A. Genes to Cells

（2011.9.21）2. “Micro-scale phosphoproteome analysis of 10,000 cells from human

cancer cell lines” Masuda, T., Sugiyama, N., Tomita, M. and Ishihama, Y. Anal. Chem., DOI:10.1021/ac201093g (2011.9.8)

3. “Non-targeted metabolite profiling in activated macrophage secretion” Sugimoto, M., Sakagami, H., Yokote, Y., Onuma, H., Kaneko, M., Mori, M., Sakaguchi, Y., Soga, T. and Tomita, M. Metabolomics DOI: 10.1007/s11306-011-0353-9(2011.8.30)

4. “A novel three-unit tRNA splicing endonuclease found in ultrasmall archaea possesses broad substrate specificity” Fujishima, K., Sugahara, J., Miller, CS., Baker BJ., Di Giulio, M., Takesue, K., Sato, A., Tomita, M., Banfield, JF. and Kanai, A. Nucl. Acids Res. doi: 10.1093/nar/gkr692 (2011.8.8) [Epub ahead of print]

5. "Predicting the kinetic properties associated with redox imbalance after oxidative crisis in G6PD-deficient erythrocytes: a simulation study" Shimo, H., Nishino, T. and Tomita, M. Advances in Hematolog (2011.6.17)

6. “Peptide Identification by Searching Large-Scale Tandem Mass Spectra against Large Databases: Bioinformatics Methods in Proteogenomics” Helmy, M., Tomita, M. and Ishihama, Y. GGG (2011.5.6)

7. “Profiling of the Charged Metabolites of Traditional Herbal Medicines Using Capillary Electrophoresis Time-of-Flight Mass Spectrometry” Iino¸ K., Sugimoto¸ M., Soga¸ T. and Tomita, M. Metabolomics DOI: 10.1007/s11306-011-0290-7 (2011)

Published8. “Deep sequencing reveals as-yet-undiscovered small RNAs in

Escherichia coli” Shinhara, A., Matsui, M., Hiraoka, K., Nomura, W., Hirano, R., Nakahigashi, K., Tomita, M., Mori, H. and Kanai, A. BMC Genomics 12:428 (2011.)

9. “Tight Associations between Transcription Promoter Type and Epigenetic Variation in Histone Positioning and Modification” Nozaki, T., Yachie, N., Ogawa, R., Kratz, A., Saito, R. and Tomita, M. BMC Genomics 12:416

(2011)10. "Metabolic flux analysis and visualization" Toya, Y., Kono, N., Arakawa, K.

and Tomita, M. J. Proteome Res. 10 : 3313–3323 (2011)11. “Chlamydomonas neoplanoconvexa nom. nov. and i ts unique

phylogenetic position within Volvocales (Chlorophyceae)” Nakada, T. and Tomita, M. Phycol. Res. 59: 194–199 (2011)

12. “KBWS: an EMBOSS associated package for accessing bioinformatics web services” Oshita, K., Arakawa, K. and Tomita, M. Source Code Biol Med. 6:8 (2011)

13. “A screening system for artificial small RNAs that inhibit the growth of Escherichia coli” Komasa, M., Fujishima, K., Hiraoka, K., Shinhara, A., Lee, Baek-Seok., Tomita, M. and Kanai, A. J. Biochem. 150:289–294 (2011)

14. “Comprehensive expressional analyses of antisense transcripts in colon cancer tissues using artificial antisense probes” Saito, R., Kohno, K., Okada, Y., Osada, Y., Numata, K., Kohama, C., Watanabe, K., Nakaoka, H., Yamamoto, N., Kanai, A., Yasue, H., Murata, S., Abe, K., Tomita, M., Ohkohchi, N. and Kiyosawa, H. BMC Med. Genomics. 4:42 (2011)

15. “Redox Regulation in Respiring Saccharomyces cerevisiae” Murray, D.B., Haynes, K. and Tomita, M. Biochim Biophys Acta. 1810:945-58 (2011)

16. “Positive Autoregulation Delays the Expression Phase of Mammalian Clock Gene Per2” Ogawa, Y., Koike, N., Kurosawa, G., Soga, T., Tomita, M. and Tei, H. PLoS ONE 6: e18663 (2011)

17. “Predictive value of CD24 and CD44 for neoadjuvant chemotherapy response and prognosis in primary breast cancer patients” Horiguchi, K., Toi, M., Horiguchi, S., Sugimoto, M., Naito, Y., Hayashi, Y., Ueno, T., Ohno, S., Funata, N., Kuroi, K., Tomita, M. and Eishi1, Y. J. Med. Dent. Sci. 57：165 － 175 (2010)

18. “OryzaPG-DB: R ice Proteome Database based on Shotgun Proteogenomics” Helmy, M., Tomita, M. and Ishihama, Y. BMC Plant Biol. 11:63 (2011)

19. “Computational analysis suggests a highly bendable, fragile structure for nucleosomal DNA” Nozaki, T., Yachie, N., Ogawa, R., Saito, R. and Tomita, M. Gene 476: 10-14 (2011)

20. “Serum Metabolomics Reveals γ -glutamyl dipeptides as biomarkers for discrimination among different forms of liver disease” Soga, T., Sugimoto, M., Honma, M., Mori, M., Igarashi, K., Kashikura, K., Ikeda, S., Hirayama, A., Yamamoto, T., Yoshida, H., Otsuka, M., Tsuji, S., Yatomi, Y., Sakuragawa, T., Watanabe, H., Nihei, K., Saito, T., Kawata, S., Suzuki, H., Tomita, M. and Suematsu, M. J. Hepatol. 55:896-905 (2011)

21. “Metabolomic Anatomy of an Animal Model revealing homeostatic imbalances in dyslipidemia” Ooga, T., Sato, H., Nagashima, A., Sasaki, K., Tomita, M., Soga T. and Ohashi, Y. Mol. BioSyst. 7:1217–1223 (2011)

22. “Integrative features of the yeast phosphoproteome and protein-protein interaction map” Yachie, N., Saito, R., Sugiyama, N., Tomita, M. and Ishihama, Y. PLoS Comput Biol. 7:e1001064(2011)

23. “Comprehensive prediction of chromosome dimer resolution sites in bacterial genomes” Kono, N., Arakawa, K. and Tomita, M. BMC Genomics 12:19 (2011)

24. “A novel mechanism regulates H2S and SO2 production in Saccharomyces cerevisiae” Yoshida, S., Imoto, J., Minato, T., Oouchi, R., Kamada, Y., Tomita, M., Soga, T. and Yoshimoto, H. Yeast 28: 109–121 (2011)

柳川弘志／土居信英

(2007 年度 )1. Nishizaki, T., Tsuge, K., Itaya, M., Doi, N., Yanagawa, H.: Metabolic

engineering of carotenoid biosynthesis in Escherichia coli by ordered gene assembly in Bacillus subtilis (OGAB). Appl. Environ. Microbiol. 73, 1355-1361 (2007)

2. Doi, N., Takashima, H., Wada, A., Oishi, Y., Nagano, T., Yanagawa, H.: Photocleavable linkage between genotype and phenotype for rapid and efficient recovery of nucleic acids encoding affinity-selected proteins. J. Biotechnol., 131, 231-239 (2007)

3. Kawahashi, Y., Doi, N., Oishi, Y., Tsuda, C., Takashima, H., Baba, T., Mori, H., Ito, T., Yanagawa, H.: High-throughput fluorescence labeling of full-length cDNA products based on a reconstituted translation system. J. Biochem. 141, 19-24 (2007)

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(2008 年度 )1. Horisawa, K., Doi, N., Yanagawa, H.: Use of cDNA tiling arrays for

identifying protein-interactions selected by in vitro display technologies. PLoS ONE 3, e1646 (2008)

2. Kosugi, S., Hasebe, M., Entani, T., Takayama, S., Tomita, M., Yanagawa, H.: Design of peptide inhibitors for importin α / β nuclear import pathway by activity-based profiling. Chem. Biol., 15, 940-949 (2008)

3. Kosugi, S., Hasebe, M., Tomita, M., Yanagawa, H.: Nuclear export signal consensus sequences defined using a localization-based yeast selection system, Traffic, 9, 2053-2062 (2008)

4. Tsuji, T., Nagata, T., Yanagawa, H.: N- and C-terminal fragments of a globular protein constructed by elongation of modules as units associated for functional complementation. J. Biochem., 144, 513-521 (2008)

(2009 年度 )1. Tabata, N., Sakuma, Y., Honda, Y., Doi, N., Takashima, H., Miyamoto-

Sato, E., Yanagawa, H.: Rapid antibody selection by mRNA display on a microfluidic chip. Nucleic Acids Res., 37, e64 (2009)

2. Sumida, T., Doi, N., Yanagawa, H.: Bicistronic DNA display for in vitro selection of Fab fragments . Nucleic Acids Res., 37, e147 (2009)

3. Tsuji, T., Onimaru, M., Doi, N., Miyamoto-Sato, E., Takashima, H., Yanagawa, H.: In vitro selection of GTP-binding proteins by block shuffling of estrogen-receptor fragments. Biochem. Biophys. Res. Commun., 390, 689-693 (2009)

4. Kosugi, S., Hasebe, M., Tomita, M., Yanagawa, H.: Systematic identification of cell cycle-dependent nucleocytoplasmic shuttling proteins by prediction of composite motifs. Proc. Natl. Acad. Sci. USA, 106, 10171-10176 (2009)

5. Kosugi, S., Hasebe, M., Matsumura, N., Takashima, H., Miyamoto-Sato, E., Tomita, M., Yanagawa, H.: Six classes of nuclear localization signals specific to different binding grooves of importin alpha. J. Biol. Chem., 284, 478-485 (2009)

6. Suzuki, H. 他 160 名 , Yanagawa, H. (135 番 目 ): The transcriptional network that controls growth arrest and differentiation in a human myeloid leukemia cell line. Nature Genet., 41, 553-562 (2009)

7. Horisawa, K., Imai, T., Okano, H., Yanagawa, H.: 3'-Untranslated region of doublecortin mRNA is a binding target of the Musashi1 RNA-binding protein. FEBS Lett. 583, 2429-2434 (2009)

8. Horisawa, K., Imai, T., Okano, H., Yanagawa, H.: The Musashi family RNA-binding proteins in stem cells. Biomol. Concepts, 1, 59-66 (2010)

9. Miyamoto-Sato, E., Ishizaka, M., Fujimori, S., Hirai, N., Masuoka, K., Saito, R., Ozawa, Y., Hino, K., Washio, T., Tomita, M., Yamashita, T., Oshikubo, T., Akasaka, H., Sugiyama, J., Matsumoto, Y., Yanagawa, H.: A comprehensive resource of interacting protein regions for refining human transcription factor networks: Domain-based interactome. PLoS ONE, 5, e9289 (2010)

(2010 年度 )1. Matsumura, N., Tsuji, T., Sumida, T., Kokubo, M., Onimaru, M., Doi,

N., Takashima, H., Miyamoto-Sato, E., Yanagawa, H.: mRNA display selection of a high-affinity, Bcl-XL-specific binding peptide. FASEB J., 24, 2201-2210 (2010)

2. Tanaka, J., Doi, N., Takashima, H., Yanagawa, H.: Comparative characterization of random-sequence proteins consisting of 5, 12 and 20 kinds of amino acids. Protein Sci., 19, 786-795 (2010)

3. Chiku, M., Hor isawa, K., Doi , N., Yanagawa, H., Einaga, Y. : Electrochemical detection of tyrosine derivatives and protein tyrosine kinase activity using boron-doped diamond electrodes. Biosens. Bioelectron., 26, 235-240 (2010)

4. Shiheido, H., Takashima, H., Doi, N., Yanagawa, H.: mRNA display selection of an optimized MDM2-binding peptide that potently inhibits MDM2-p53 interaction. PLoS ONE, 6, e17898 (2011)

5. Tanaka, J., Yanagawa, H., Doi, N.: Comparison of the frequency of functional SH3 domains with different limited sets of amino acids using mRNA display. PLoS ONE, 6, e18034 (2011)

6. 【図書】柳川弘志，土居信英：生体高分子の進化工学　現代生物科学入門９：合成生物学　 岩波書店 (2010)

（2011 年度）1. Doi, N., Kakukawa, K., Oishi, Y., Yanagawa, H.: High Solubility of

Random-Sequence Proteins Consisting of Five Kinds of Primitive Amino Acids. In: Chemical Synthetic Biology, Luisi, L. and Chiarabelli, C. (Eds.), pp. 121-137, A John Wiley & Sons, UK (2011)

2. Tanaka, J., Yanagawa, H., Doi, N.: Evolutionary Engineering of Artificial Proteins with Limited Sets of Primitive Amino Acids. In: Protein Engineering, InTech, Rijeka, Croatia (2011)

瀬藤光利

(2009 年度 )1. Konishi Y, Setou M. Tubulin tyrosination navigates the kinesin-1 motor

domain to axons. Nat Neurosci. 12, 559-567, 2009.2. Harada T, Yuba-Kubo A, Sugiura Y, Zaima N, Hayasaka T, Goto-Inoue

N, Wakui M, Suematsu M, Takeshita K, Ogawa K, Yoshida Y, Setou M. Visualization of volatile substances in different organelles with an atmospheric-pressure mass microscope. Anal Chem. 81, 9153-9157, 2009.

3. Tada H, Okano HJ, Takagi H, Shibata S, Matsumoto M, Yao I, Saiga T, Nakayama KI, Kashima H, Takahashi T, Setou M, Okano H. Fbxo45, a novel ubiquitin ligase, regulates synaptic activity. J Biol Chem. 285, 3840-3849, 2010.

(2010 年度 )1. Shrivas K, Hayasaka T, Goto-Inoue N, Zaima N, Setou M. Ionic matrix

for enhanced MALDI imaging mass spectrometry for identification of phospholipids in mouse liver and cerebellum tissue sections. Anal Chem. 82, 8800–8806, 2010.

2. Kimura Y, Kurabe N, Ikegami K, Tsutsumi K, Konishi Y, Kaplan OI, Kunitomo H, Iino Y, Blacque OE, Setou M. Identification of Tubulin Deglutamylase among Caenorhabditis elegansand Mammalian Cytosolic Carboxypeptidases (CCPs). J Biol Chem. 285, 22936-22941, 2010.

3. Ikegami K, Sato S, Nakamura K, Ostrowski LE, Setou M. Tubulin polyglutamylation is essential for airway ciliary function through the regulation of beating asymmetry. Proc Natl Acad Sci U S A. 107, 10490-10495, 2010.

（2011 年度）1. Shrivas K, Hayasaka T, Sugiura Y, Setou M. Method for Simultaneous

Imaging of Endogenous Low Molecular Weight Metabolites in Mouse Brain Using TiO(2) Nanoparticles in Nanoparticle-Assisted Laser Desorption/Ionization-Imaging Mass Spectrometry. Anal Chem. 83, 7283–7289, 2011.

2. Yuki D, Sugiura Y, Zaima N, Akatsu H, Hashizume Y, Yamamoto T, Fujiwara M, Sugiyama K, Setou M. Hydroxylated and non-hydroxylated sulfatide are distinctly distributed in the human cerebral cortex. Neuroscience. 193, 44-53, 2011

3. Sugiura Y, Taguchi R, Setou M. Visualization of spatiotemporal energy dynamics of hippocampal neurons by mass spectrometry during a kainate-induced seizure. PLoS One. 6, e17952, 2011.

仲嶋一範

(2007 年度 )1. Takuya T Maeda, Itsuki Ajioka, and Kazunori Nakajima. Computational

cell model based on autonomous cell movement regulated by cell-cell signalling successfully recapitulates the “inside and outside” pattern of cell sorting. BMC Syst. Biol., 1, 43 (16 pages) (2007).

2. Kazunori Nakajima. Control of tangential/non-radial migration of neurons in the developing cerebral cortex. Neurochem. Int. ,51 (2-4), 121-131 (2007).

3. Yoshimi Nakano, Takao Kohno, Terumasa Hibi, Shiori Kohno, Atsushi Baba, Katsuhiko Mikoshiba, Kazunori Nakajima, and Mitsuharu Hattori.　The extremely conserved C-terminal region of Reelin is not necessary for secretion but is required for efficient activation of downstream signaling. J. Biol. Chem., 282 (28), 20544-20552 (2007).

4. Toshio Ohshima, Motoyuki Hirasawa, Hidenori Tabata, Tetsuji Mutoh,

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Tomoko Adachi, Hiromi Suzuki, Keiko Saruta, Takuji Iwasato, Shigeyoshi Itohara, Mitsuhiro Hashimoto, Kazunori Nakajima, Masaharu Ogawa, Ashok B. Kulkarni and Katsuhiko Mikoshiba. Cdk5 is required for multipolar-to-bipolar transition during radial neuronal migration and proper dendrite development of pyramidal neurons in the cerebral cortex. Development, 134 (12), 2273-2282 (2007).

5. Yutaka Sato, Masato Taoka, Nami Sugiyama, Kenichiro Kubo, Takahiro Fuchigami, Akiko Asada, Taro Saito, Kazunori Nakajima, Toshiaki Isobe, and Shin-ichi Hisanaga. Regulation of the interaction of Disabled-1 with CIN85 by phosphorylation with Cyclin-dependent kinase 5. Genes to Cells, 12 (12), 1315-1327 (2007).

6. Kashiko Tachikawa, Shinji Sasaki, Takuya Maeda, and Kazunori Nakajima. Identification of molecules preferentially expressed beneath the marginal zone in the developing cerebral cortex. Neurosci. Res., 60 (2), 135-146 (2008).

7. Masato Yozu, Hidenori Tabata, Norbert Koenig, and Kazunori Nakajima. Migratory behavior of presumptive interneurons is affected by AMPA receptor activation in slice cultures of embryonic mouse neocortex. Dev. Neurosci. , 30 (1-3), 105-116 (2008).

8. 関根克敏、仲嶋一範 ; リーリン , KEY WORD 精神 　第 4 版、先端医学社、228-229 (2007).

9. 田畑秀典、仲嶋一範 一一; バイオテクノロジージャーナル 、7 (4)、489-495 (2007).

10. 宇都飛鳥 , 仲嶋一範 ; 神経細胞の移動と層構造形成の制御機構 , 蛋白質 核酸 酵素 増刊号「神経の分化、回路形成、機能発現」、共立出版、53 (4), 365-372 (2008).

（2008 年度）1. Barbara Carletti, Ian M Williams, Ketty Leto, Kazunori Nakajima, Lorenzo

Magrassi, and Ferdinando Rossi. Time constraints and positional cues in the developing cerebellum regulate Purkinje cell placement in the cortical architecture. Dev. Biol., 317 (1), 147-160 (2008).

2. Gaëlle Friocourt, Shigeaki Kanatani, Hidenori Tabata, Masato Yozu, Takao Takahashi, Mary Antypa, Odile Raguénès, Jamel Chelly, Claude Férec, Kazunori Nakajima, and John G. Parnavelas.　Cell-autonomous roles of ARX in cell proliferation and neuronal migration during corticogenesis. J. Neurosci., 28 (22), 5794-5805 (2008). (G. Friocourt and S. Kanatani are co-first authors.)

3. Atsushi Kamiya, Perciliz L. Tan, Ken-ichiro Kubo, Caitlin Engelhard, Koko Ishizuka, Akiharu Kubo, Sachiko Tsukita, Ann E. Pulver, Kazunori Nakajima, Nicola G. Cascella, Nicholas Katsanis, and Akira Sawa.　Recruitment of PCM1 to the centrosome by the cooperative action of DISC1 and BBS4: a candidate for psychiatric illnesses. Arch. Gen. Psychiatry, 65 (9), 996-1006 (2008) .

4. Shigeaki Kanatani, Masato Yozu, Hidenori Tabata, and Kazunori Nakajima. COUP-TFII is preferentially expressed in the caudal ganglionic eminence and is involved in the caudal migratory stream. J. Neurosci.,28 (50), 13582-13591 (2008).

5. Shinji Sasaki, Hidenori Tabata, Kashiko Tachikawa, and Kazunori Nakajima. The cortical subventricular zone-specific molecule Svet1 is part of the nuclear RNA coded by the putative Netrin receptor gene Unc5d and is expressed in multipolar migrating cells. Mol. Cell. Neurosci., 38 (4), 474-483 (2008).

6. Hidenori Tabata and Kazunori Nakajima. 　Labeling embryonic mouse central nervous system cells by in utero electroporation. Develop. Growth Differ., 50 (6),507-511 (2008).　

7. Koji Oishi and Kazunori Nakajima. Neurogenesis. Encyclopedia of Neuroscience (Editors: Marc D. Binder, Nobutaka Hirokawa and Uwe Windhorst), Springer, 2673-2676 (2009).

8. Hidenori Tabata and Kazunori Nakajima. In utero electroporation; assay system for migration of cerebral cortical neurons, Electroporation and Sonoporation in Developmental Biology (Editor: Harukazu Nakamura), Springer, 143-152 (2009).

9. 小林和馬、仲嶋一範；大脳皮質構築のメカニズム、特集「神経系の発生とその異常」（ゲストエディター：仲嶋一範）、BRAIN and NERVE、60（4）、395-403 (2008).

10. 仲嶋一範；発生期大脳皮質を接線方向に移動するニューロンの起源と移動経路の制御機構、実験医学 増刊「神経回路の制御と脳機能発現のメカニズム」、26 (12)、1847-1851 （2008）.

11. 仲嶋一範；発生期大脳皮質における移動神経細胞のダイナミクス、ブレ

インサイエンス・レビュー 2009（ブレインサイエンス振興財団 伊藤正男・川合述史 編）、クバプロ、207-232 (2009).

（2009 年度）1. Haruo Okado, Chiaki Ohtaka-Maruyama, Yoshinobu Sugitani, Yuko

Fukuda, Reiko Ishida, Shinobu Hirai, Akiko Miwa, Akiyo Takahashi, Katsunori Aoki, Keijo Mochida, Osamu Suzuki, Takao Honda, Kazunori Nakajima, Masaharu Ogawa, Toshio Terashima, Junichiro Matsuda, Hitoshi Kawano, and Masataka Kasai. The transcriptional repressor RP58 is crucial for proper cell-division patterning and neuronal survival in the developing cortex. Dev. Biol., 331 (2), 140-151 (2009).

2. Ko j i O ish i , Ken j i Wata tan i , Yasuh i ro I toh , H ideyuk i Okano, François Guillemot, Kazunori Nakajima, and Yukiko Gotoh. Selective induction of neocortical GABAergic neurons by the PDK1-Akt pathway through activation of Mash1. Proc. Natl. Acad. Sci. U.S.A., 106 (31), 13064-13069 (2009).

3. Hidenori Tabata, Shigeaki Kanatani, and Kazunori Nakajima. Differences of migratory behavior between direct progeny of apical progenitors and basal progenitors in the developing cerebral cortex. Cereb. Cortex, 19 (9), 2092-2105 (2009).

4. Takayuki Uchida, Atsushi Baba, F. Javier Pérez-Martínez, Terumasa Hibi, Takaki Miyata, Juan M. Luque, Kazunori Nakajima, and Mitsuharu Hattori. Downregulation of functional Reelin receptors in projection neurons implies that primary Reelin action occurs at early/pre-migratory stages. J. Neurosci., 29 (34), 10653-10662 (2009).

5. Kaoru Eto, Takeshi Kawauchi, Makiko Osawa, Hidenori Tabata, and Kazunori Nakajima. Role of dual leucine zipper-bearing kinase (DLK/MUK/ZPK) in axonal growth. Neurosci. Res., 66 (1), 37-45 (2010).

6. Yoshiaki V. Nishimura, Katsutoshi Sekine, Kaori Chihama, Kazunori Nakajima, Mikio Hoshino, Yo-ichi Nabeshima, and Takeshi Kawauchi, Dissecting the factors involved in the locomotion mode of neuronal migration in the developing cerebral cortex. J. Biol. Chem., 285 (8), 5878-5887 (2010).

7. Minae Niwa, Atsushi Kamiya, Rina Murai, Ken-ichiro Kubo, Aaron J Gruber, Kenji Tomita, Lingling Lu, Shuta Tomisato, Hanna Jaaro-Peled, Saurav Seshadri, Hideki Hiyama, Beverly Huang, Kazuhisa Kohda, Yukihiro Noda, Patricio OʼDonnell, Kazunori Nakajima, Akira Sawa, and Toshitaka Nabeshima. Knockdown of DISC1 by in utero gene transfer disturbs postnatal dopaminergic maturation in the frontal cortex and leads to adult behavioral deficits. Neuron, 65 (4), 480-489 (2010).

（2010 年度）1. Shigeo Uchino, Takae Hirasawa, Hidenori Tabata, Yuko Gonda,

ChikakoWaga, Yumiko Ondo, Kazunori Nakajima, and Shinichi Kohsaka. Inhibition of N-methyl-d-aspartate receptor activity resulted in aberrant neuronal migration caused by delayed morphological development in the mouse neocortex. Neuroscience, 169 (2), 609-618 (2010). (S. Uchino, T. Hirasawa, and H. Tabata are co-first authors.)

2. Takeshi Kawauchi, Katsutoshi Sekine, Mima Shikanai, Kaori Chihama, Kenji Tomita, Ken-ichiro Kubo, Kazunori Nakajima, Yo-ichi Nabeshima, and Mikio Hoshino. Rab GTPases-dependent endocytic pathways regulate neuronal migration and maturation through N-Cadherin trafficking. Neuron, 67 (4), 588-602 (2010).

3. Ken-ichiro Kubo, Takao Honda, Kenji Tomita, Katsutoshi Sekine, Kazuhiro Ishii, Asuka Uto, Kazuma Kobayashi, Hidenori Tabata, and Kazunori Nakajima. Ectopic Reelin induces neuronal aggregation with a normal birthdate-dependent "inside-out" alignment in the developing neocortex. J. Neurosci., 30 (33), 10953-10966 (2010).

4. Ken-ichiro Kubo, Kenji Tomita, Asuka Uto, Keisuke Kuroda, Saurav Seshadri, Jared S. Cohen, Kozo Kaibuchi, Atsushi Kamiya, and Kazunori Nakajima. Migration defects by DISC1 knockdown in C57BL/6, 129X1/SvJ, and ICR strains via in utero gene transfer and virus-mediated RNAi. Biochem. Biophys. Res. Commun., 400 (4), 631-637 (2010). (K. Kubo and K. Tomita are co-first authors).

5. Kazunobu Sawamoto, Yuki Hirota, Clara Alfaro-Cervello, Mario Soriano-Navarro, Xiaoping He, Yoshika Hayakawa-Yano, Masayuki Yamada, Keigo Hikishima, Hidenori Tabata, Akio Iwanami, Kazunori Nakajima, Yoshiaki Toyama, Toshio Itoh, Arturo Alvarez-Buylla, Jose Manuel Garcia-Verdugo, and Hideyuki Okano. Cellular composition and organization of the subventricular zone and rostral migratory stream in the adult and

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neonatal common marmoset brain. J. Comp. Neurol., 519 (4), 690-713 (2011). (K. Sawamoto, Y. Hirota, C. Alfaro-Cervello, M. Soriano-Navarro contributed equally to this work).

6. Daisuke H. Tanaka, Sakae Mikami, Takashi Nagasawa, Jun-ichi Miyazaki, Kazunori Nakajima, and Fujio Murakami. CXCR4 is required for proper regional and laminar distribution of cortical somatostatin-, calretinin- and neuropeptide Y-expressing GABAergic interneurons. Cereb. Cortex, 20 (12), 2810-2817 (2010).

7. Yuichi Tamura, Keisuke Matsumura, Motoaki Sano, Hidenori Tabata, Kensuke Kimura, Masaki Ieda, Takahide Arai, Yohei Ohno, Hideaki Kanazawa, Shinsuke Yuasa, Ruri Kaneda, Shinji Makino, Kazunori Nakajima, Hideyuki Okano, and Keiichi Fukuda. Neural crest-derived stem cells migrate and differentiate into cardiomyocytes after myocardial infarction, Arterioscler. Thromb. Vasc. Biol., 31 (3), 582-589 (2011).

8. 澤明、仲嶋一範 ; DISC1 研究の期待と課題 —精神疾患研究のマイルストーンの１つとして , 実験医学 、29 (1)、94-102 (2011).

（2011 年度）1. Makoto Takemoto, Yuki Hattori, Hong Zhao, Haruka Sato, Atsushi

Tamada, Shinji Sasaki, Kazunori Nakajima, and Nobuhiko Yamamoto. Laminar and areal expression of Unc5d and its role in cortical cell survival. Cereb. Cortex, 21 (8), 1925-1934 (2011).

2. Koko Ishizuka, Atsushi Kamiya, Edwin C. Oh, Hiroaki Kanki, Saurav Seshadri, Jon F. Robinson, Hannah Murdoch, Allan J. Dunlop, Ken-ichiro Kubo, Keiko Furukori, Beverly Huang, Mariela Zeledon, Akiko Hayashi-Takagi, Hideyuki Okano, Kazunori Nakajima, Miles D. Houslay, Nicholas Katsanis, and Akira Sawa. DISC1-dependent switch from progenitor proliferation to migration in the developing cortex. Nature, 473 (7345), 92-96 (2011).

3. Takao Honda, Kazuma Kobayashi, Katsuhiko Mikoshiba, and Kazunori Nakajima. Regulation of cortical neuron migration by the Reelin signaling pathway. Neurochem. Res., 36 (7), 1270-1279 (2011).

4. Yee Ping Yip, Guangdou Zhou, Ken-ichiro Kubo, Kazunori Nakajima, and Joseph W. Yip. Reelin inhibits migration of sympathetic preganglionic neurons in the spinal cord of the chick. J. Comp. Neurol., 519 (10), 1970-1978 (2011).

5. Mima Shikanai, Kazunori Nakajima, and Takeshi Kawauchi. N-Cadherin regulates radial glial fiber-dependent migration of cortical locomoting neurons. Communicative & Integrative Biology, 4 (2), 326-330 (2011).

6. Yongheng Cao, Masanori Nakata, Shiki Okamoto, Eisuke Takano, Toshihiko Yada, Yasuhiko Minokoshi, Yukio Hirata, Kazunori Nakajima, Kristy Iskandar, Yoshitake Hayashi, Wataru Ogawa, Gregory S. Barsh, Hiroshi Hosoda, Kenji Kangawa, Hiroshi Itoh, Tetsuo Noda, Masato Kasuga, and Jun Nakae. PDK1-Foxo1 in Agouti-related peptide neurons regulates energy homeostasis by modulating food intake and energy expenditure. PLoS ONE, 6(4), e18324 (2011). (doi:10.1371/journal.pone.0018324)

7. Daisuke H. Tanaka, Ryo Oiwa, Erika Sasaki, and Kazunori Nakajima. Changes in cortical interneuron migration contribute to the evolution of the neocortex. Proc. Natl. Acad. Sci. U.S.A., 108 (19), 8015-8020 (2011).

8. Kenj i Tomi ta, Ken- ichiro Kubo, Kazuhiro Ishi i , and Kazunor i Nakaj ima. Disrupted-in-Schizophrenia-1 (Disc1) is necessary for migration of the pyramidal neurons during mouse hippocampal development. Hum. Mol. Genet., 20 (14), 2834-2845 (2011). (K. Tomita and K. Kubo are co-first authors)

9. Katsutoshi Sekine, Takao Honda, Takeshi Kawauchi, Ken-ichiro Kubo, and Kazunori Nakajima. The outermost region of the developing cortical plate is crucial for both the switch of the radial migration mode and the Dab1-dependent “inside-out” lamination in the neocortex. J. Neurosci., 31 (25), 9426-9439 (2011). (cover illustration)

10. Itsuki Ajioka, Shizuko Ichinose, Kazunori Nakajima, and Hidehiro Mizusawa. Basement membrane-like matrix sponge for the three-dimensional proliferation culture of differentiated retinal horizontal interneurons. Biomaterials, 32 (25), 5765-5772 (2011).

11. Katsutoshi Sekine, Hidenori Tabata, and Kazunori Nakajima. Cell polarity and initiation of migration (Chapter 24). Developmental Neuroscience: A Comprehensive Reference, Elsevier, in press.

12. Daisuke H. Tanaka, Kazuya Toriumi, Ken-ichiro Kubo, Toshitaka Nabeshima, and Kazunor i Nakaj ima. GABAergic precursor transplantation into the prefrontal cortex prevents phencyclidine-induced

cognitive deficits. J. Neurosci., 31 (40), 14116-14125 (2011).13. Mitsuaki Nishikimi, Koji Oishi, Hidenori Tabata, Kenichi Torii, and Kazunori

Nakajima. Segregation and pathfinding of callosal axons through EphA3 signaling. J. Neurosci., in press.

14. 仲嶋一範 ; 大脳皮質の層形成「制御」因子リーリンは，何を「制御」しているのか？（ミニレビュー） 生化学 、83 (8)、727-731 (2011).

15. Hidenor iTabata, SatoshiYoshinaga, and Kazunor i Nakaj ima.Cytoarchitecture of mouse and human subventricular zone in developing cerebral neocortex.Exp. Brain Res., in press.

16. 久保健一郎、仲嶋一範 ; 海馬と統合失調症、分子精神医学 、印刷中

澤本和延

(2007 年度 )1. Adachi, K., Mirzadeh, Z., Sakaguchi, M., Yamashita, T., Nikolcheva, T.,

Gotoh, Y., Peltz, G., Gong, L., Kawase, T., Alvarez-Buylla, A., Okano, H. and Sawamoto, K. (2007) β-catenin signaling promotes proliferation of progenitor cells in the adult mouse subventricular zone. Stem Cells 25, 2827-2836.

2. Hirota, Y., Ohshima, T., Kaneko, N., Ikeda, M., Iwasato, T., Kulkarni, A.B., Mikoshiba, K., Okano, H. and Sawamoto, K. (2007) Cyclin-dependent kinase 5 is required for control of neuroblast migration in the postnatal subventricular zone. J. Neurosci. 27: 12829-12838.

3. Okano, H., Sakaguchi, M., Ohki, K., Suzuki, N. and Sawamoto, K. (2007) Regeneration of the central nervous system using endogenous repair mechanisms. J Neurochem. 102: 1459-1465. (Invited review)

(2008 年度 )1. Kaneko, N. and Sawamoto, K. (2009) Adult neurogenesis and its

alteration under pathological conditions. Neurosci. Res. 63: 155-164. (Invited review)

2. Kohyama, J., Kojima, T., Takatsuka, E., Yamashita, T., Namiki, J., Hsieh, J., Gage, F.H., Namihira, M., Okano, H., Sawamoto, K. and Nakashima, K. (2008) Epigenetic regulation of neural cell differentiation plasticity in the adult mammalian brain. Proc. Natl. Acad. Sci. USA. 105, 18012-18017.

3. Okano, H. and Sawamoto, K. (2008) Neural stem cells: Involvement in adult neurogenesis and CNS repair. Philos. Trans. R. Soc. B. Biol. Sci. 363: 2111-2122. (Invited review)

4. Sunabori, T., Tokunaga, A., Nagai, T., Sawamoto, K., Okabe, M., Miyawaki, A., Matsuzaki, Y., Miyata, T. and Okano, H. (2008) Cell-cycle-specific nestin expression coordinates with morphological changes in embryonic cortical neural progenitors. J. Cell Sci. 121, 1204-1212.

5. Suzuki, T., Miyamoto, H., Nakahari, T., Inoue, I., Suemoto, T., Jiang, b., Hirota, Y., Itohara, S., Saido, T.C., Tsumoto, T., Sawamoto, K., Hensch, T.K., Delgado-Escueta, A.V. and Yamakawa, K. (2009) Efhc1 deficiency causes spontaneous myoclonus and increased seizure susceptibility. Hum. Mol. Genet. 18: 1099-1109.

6. 黄詩恵、廣田ゆき、澤本和延 発達期における上衣細胞繊毛の成熟と脳脊髄液循環．小児の脳神経 34（1）；10-15 (2009)

7. 金子奈穂子、澤本和延 成体脳におけるニューロンの新生と移動・成熟 . 日本神経精神薬理学雑誌 28(281)：63-68 (2008)

8. 金子奈穂子、澤本和延 成体脳におけるニューロン新生と脳梗塞後の神経新生メカニズム . 実験医学増刊　神経回路の制御と脳機能発現のメカニズム 　26：224-229 (2008)

9. 金子奈穂子、澤本和延 成体脳のニューロン新生とその異常 . Brain and Nerve 60(4): 319-328, (2008)

10. 小島拓郎、廣田ゆき、澤本和延 成体脳におけるニューロン新生 . 慶應医学 85(2)169-177 （2009）

11. 廣田ゆき、澤本和延 ニューロンの移動制御と細胞接着 . 実験医学 26（14）；2191-2195 (2008)

12. 廣田ゆき、澤本和延 成体脳室下帯で誕生し移動するニューロン . 蛋白質核酸酵素 53(7): 863-869 (2008)

13. 澤本和延 新規 GSK3b インヒビターによって明らかになった成体神経前駆細胞の増殖における Wnt/ β -catenin シグナルの機能．実験医学 27(5)増刊号　分子標的薬開発への新たなる挑戦；69-73 （2009）　編集／岡野栄之、岩坪威、佐谷秀行

14. 澤本和延、岸本憲人 . 脳室壁付近に存在する成体神経幹細胞ニッチの機能と進化 . 細胞工学 27(7)：681-685 (2008)

15. 澤本和延、金子奈穂子、鈴木重明 . 脳梗塞後の神経再生：基礎研究の現

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List of Achievem

ents

状と臨床応用への課題 . 再生医療 7（4）；19-25 (2008)

(2009 年度 )1. Guirao, B., Meunier, A., Mortaud, S., Aguilar, A., Corsi, J.M., Strehl, L.,

Hirota, Y., Desoeuvre, A., Boutin, C., Han, Y.G., Mirzadeh, Z., Cremer, H., Montcouquiol, M., Sawamoto, K. and Spassky N. (2010) Coupling between hydrodynamic forces and planar cell polarity orients mammalian motile cilia. Nat. Cell Biol. 12: 341-350.

2. Kojima, T., Hirota, Y., Ema, M., Takahashi, S., Miyoshi, I., Okano, H. and Sawamoto, K. (2010) Subventricular zone-derived neural progenitor cells migrate along a blood vessel scaffold toward the post-stroke striatum. 28: 545-554.

3. Oki, K., Kaneko, N., Kanki, H., Imai, T., Suzuki, N., Sawamoto, K. and Okano, H. (2010) Musashi1 as a marker of reactive astrocytes after transient focal brain ischemia Neurosci. Res. 66(4): 390-395.

4. Tamase, A., Muraguchia, T., Naka, K., Tanaka, S., Kinoshita, M., Hoshii, T., Ohmura, M., Shugo, H., Ooshioa, T., Nakada, M., Sawamoto, K., Onodera, M., Matsumoto, K., Oshima, M., Asano, M., Saya, H., Okano, H., Suda, T., Hamada, J. and Hirao, A. (2009) Identification of tumor-initiating cells in a highly aggressive brain tumor using promoter activity of nucleostemin　Proc. Natl. Acad. Sci USA. 106: 18012-18017.

5. Enomoto, A., Asai, N., Namba, T., Wang, Y., Kato, T., Tanaka, M. Tatsumi, H., Taya, S., Tsuboi, D., Kuroda, K., Kaneko, N., Sawamoto, K., Miyamoto, R. Jijiwa, M., Murakumo, Y., Sokabe, M., Seki,T., Kaibuchi, K., Takahashi, M. (2009) Roles of Disrupted-In-Schizophrenia 1-Interacting Protein Girdin in Postnatal Development of the Dentate Gyrus　Neuron 63: 774-787.

6. Huang, S., Hirota, Y. and Sawamoto, K. (2009) Various facets of vertebrate cilia: motility, signaling, and role in adult neurogenesis Proceedings of the Japan Academy, Ser. B 85: 324-336. (Invited review)

7. 澤本和延 幹細胞ニッチ . 田畑泰彦編 . 遺伝子医学 MOOK 別冊ますます重要になる細胞周辺環境の科学技術－細胞の生存、増殖、機能のコントロールから再生医療まで－ ；267-273, 2009

8. 金子奈穂子 , 澤本和延 海馬ニューロンの新生と精神 神経疾患総合リハビリテーション 38 （2）: 114-120 , 2010

9. 黄詩恵 , 廣田ゆき , 澤本和延 神経組織における繊毛の役割　細胞工学28(10): 1016-1020, 2009

10. 澤本和延 脳に内在する神経再生機構　臨床神経学 49(11): 830-833, 200911. 加古英介 , 金子奈穂子 , 祖父江和哉 , 澤本和延 細胞移植を用いない脳疾患

再生医療の可能性　生物物理化学 53（4）: 103-107 , 200912. 匹田貴夫 , 澤本和延 成体の脳組織における神経幹細胞と再生医療　医学

のあゆみ 231（11）: 1112-1116 , 200913. 小島拓郎 , 廣田ゆき , 澤本和延 成体脳におけるニューロン新生．慶應医

学 85（2）: 169-177, 2009

(2010 年度 )1. Nakaguchi, K., Masuda, H., Kaneko, N. and Sawamoto, K. (2011)

Strategies for regenerating striatal neurons in the adult brain by using endogenous neural stem cells. Neurol. Res. Int. 2011: 898012 (Invited review)

2. Imaizumi, Y., Sakaguchi, M., Miroshita, T., Ito, M., Poirier, F., Sawamoto, K. and Okano, H. (2011) Galectin-1 is expressed in early-type neural progenitor cells and down-regulates neurogenesis in the adult hippocampus. Mol. Brain Jan 27: 4:7

3. Sawamoto, K., Hirota, Y., Alfaro-Cervello, C., Soriano-Navarro, M., He, X., Hayakawa-Yano, Y., Yamada, M., Hikishima, K., Tabata, H., Iwanami, A., Nakajima, K., Toyama, Y., Ito, T., Alvarez-Buylla, A., Garcia-Verdugo, J.M. and Okano, H. (2011) Cellular composition and organization of the subventricular zone and rostral migratory stream in the adult and neonatal common marmoset brain. J. Comp. Neurol. 519: 690-713.

4. Kaneko, N., Kako, E. and Sawamoto, K. (2011) Prospects and limitations of using endogenous neural stem cells for brain regeneration. Genes 2011: 2, 107-130. (Invited review)

5. Shimada, M., Haruta, M., Niida, H., Sawamoto, K. and Nakanishi, M. (2010) Protein phosphatase 1g is responsible for dephosphorylation of histone H3 at Thr 11 after DNA damage. EMBO Rep. 11: 883-889.

6. Ikeda, M., Hirota Y, Sakaguchi M, Yamada O, Kida YS, Ogura T, Otsuka T, Okano H, Sawamoto K. (2010) Expression and proliferation-promoting role of Diversin in the neuronally committed precursor cells migrating in the adult mouse brain. Stem Cells 28: 2017-2026.

7. Hirota, Y., Meunier, A., Huang, S., Shimozawa, T., Yamada, O., Kida,

Y.S., Inoue, M., Ito, T., Kato, H., Sakaguchi, M., Sunabori, T., Nakaya, M.A., Nonaka, S., Ogura, T., Higuchi, H., Okano, H., Spassky, N. and Sawamoto, K. (2010) Planar polarity of multiciliated ependymal cells involves the anterior migration of basal bodies regulated by non-muscle myosin II. Development 137: 3037-3046.

8. Kaneko, N., Marín, O., Koike, M., Hirota, Y., Uchiyama, Y., Wu, J.Y., Lu, Q., Tessier-Lavigne, M., Alvarez-Buylla, A., Okano, H., Rubenstein, J.L. and Sawamoto, K. (2010) New neurons clear the path of astrocytic processes for their rapid migration in the adult brain. Neuron 67: 213-223.

9. Mirzadeh, Z., Doetsch, F., Sawamoto, K., Wichterle, H. and Alvarez-Buylla, A. (2010) The subventricular zone en-face: wholemount staining and ependymal flow. J. Vis. Exp. 2010: May 6;(39). pii: 1938. doi: 10.3791/1938.

10. Sakaguchi, M., Imaizumi, Y., Shingo, T., Tada, H., Hayama, K., Yamada, O., Morishita, T., Kadoya, T., Uchiyama, N., Shimazaki, T., Kuno, A., Poirier, F., Hirabayashi, J., Sawamoto, K. and Okano, H. (2010) Regulation of adult neural progenitor cells by Galectin-1/beta1 Integrin interaction. J. Neurochem. 113: 1516-1524.

11. Seki, T., Sawamoto, K., Parent, J. and Alvarez-Buylla, A. Springer. Neurogenesis in the adult brain I: Neurobiology (2011) 452 ページ

12. Seki, T., Sawamoto, K., Parent, J. and Alvarez-Buylla, A. Springer. Neurogenesis in the adult brain II: Clinical implications (2011) 250 ページ

（2011 年度）1. Kishimoto, N., Shimizu, K. and Sawamoto, K. (2011) Neuronal

regeneration in a zebrafish model of adult brain injury. Disease Models & Mechanisms in press

2. Yamane, J., Ishibashi, S., Sakaguchi, M., Kuroiwa, T., Kanemura, Y., Nakamura, M., Miyoshi, H., Sawamoto, K., Toyama, Y., Mizusawa, H. and Okano, H. (2011) Transplantation of human neural stem/progenitor cells overexpressing Galectin-1 improves functional recovery from focal brain ischemia in the Mongolian gerbil. Mol. Brain 4:35.

3. Sawada, M., Kaneko, N., Inada, H., Wake, H., Kato, Y., Yanagawa, Y., Kobayashi, K., Nemoto, T., Nabekura, J. and Sawamoto, K. (2011) Sensory input regulates spatial and subtype-specific patterns of neuronal turnover in the adult olfactory bulb. J. Neurosci. 31:11587-11596.

4. Kishimoto, N., Alfaro-Cervello, C., Shimizu, K., Asakawa, K., Urasaki, A., Nonaka, S., Kawakami, K., Garcia-Verdugo, J.M. and Sawamoto, K. (2011) Migration of neuronal precursors from the telencephalic ventricular zone into the olfactory bulb in adult zebrafish. J. Comp. Neurol. 519:3549-3565.

5. Kato, S., Aoyama, M., Kakit,a H., Hida, H., Kato, I., Ito, T., Goto, T., Hussein, M.H., Sawamoto, K., Togari, H. and Asai, K. (2011) Endogenous erythropoietin from astrocyte protects the oligodendrocyte precursor cell against hypoxic and reoxygenation injury. J. Neurosci. Res. 89:1566-1574.

6. Wang, Y., Kaneko, N., Asai, N., Enomoto, A., Isotani-Sakakibara, M., Kato, T., Asai, M., Murakumo, Y., Ota, H., Hikita, T., Namba, T., Kuroda, K., Kaibuchi, K., Ming, G.L., Song, H., Sawamoto, K. and Takahashi, M. (2011) Girdin is an intrinsic regulator of neuroblast chain migration in the rostral migratory stream of the postnatal brain. J. Neurosci. 31:8109-8122.

宮本健史

(2007 年度 )(*correspondence)1. Suzuki T, *Miyamoto T, Fujita N, Ninomiya K, Iwasaki R, Toyama Y, Suda T.

Osteoblast-specific Angiopoietin 1 overexpression increases bone mass. Biochem Biophys Res Commun. Nov 3;362(4):1019-1025. 2007

2. Ninomiya K, *Miyamoto T, Imai JI, Fujita N, Suzuki T, Iwasaki R, Yagi M, Watanabe S, Toyama Y, Suda T. Osteoclastic activity induces osteomodulin expression in osteoblasts. Biochem Biophys Res Commun. Oct 19;362(2):460-466. 2007

3. Morita K, *Miyamoto T, Fujita N, Kubota Y, Ito K, Takubo K, Miyamoto K, Ninomiya K, Suzuki T, Iwasaki R, Yagi M, Takaishi H, Toyama Y and Suda T. Reactive oxygen species induce chondrocyte hypertrophy in endochondral ossification. J Exp Med. 204(7):1613-1623. 2007

4. Yagi M, Ninomiya K, Fujita N, Suzuki T, Iwasaki R, Morita K, Hosogane N, Matsuo K, Toyama Y, Suda T, and *Miyamoto T. Induction of DC-STAMP

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by alternative activation and downstream signaling mechanisms. J Bone Miner Res. 22(7):992-1001. 2007

5. Horiuchi K, Kimura T, Miyamoto T, Takaishi H, Okada Y, Toyama Y, Blobel CP. TNF-{alpha}-Converting Enzyme (TACE/ADAM17) Inactivation in Mouse Myeloid Cells Prevents Lethality from Endotoxin Shock. J Immunol. 179(5):2686-2689. 2007

6. Niki Y, Takaishi H, Takito J, Miyamoto T, Kosaki N, Matsumoto H, Toyama Y, Tada N. Administration of cyclooxygenase-2 inhibitor reduces joint inflammation but exacerbates osteopenia in IL-1 alpha transgenic mice due to GM-CSF overproduction. J Immunol. 179(1):639-646. 2007

7. Miyamoto K, Araki KY, Naka K, Arai F, Takubo K, Yamazaki S, Matsuoka S, Miyamoto T, Ito K, Ohmura M, Chen C, Hosokawa K, Nakauchi H, Nakayama K, Nakayama KI, Harada M, Motoyama N, Suda T and Hirao A. Foxo3a is essential for maintenance of hematopoietic stem cell pool. Cell Stem Cell. 1 (1):101-112. 2007

(2008 年度 )1. Fujita, N., Imai, J., Suzuki, T., Yamada, M., Ninomiya, K., Miyamoto,

K., Iwasaki, R., Morioka, H., Matsumoto, M., Chiba, K., Watanabe, S., Suda, T., Toyama, Y., Miyamoto, T. Vascular endothelial growth factor-A is a survival factor for nucleus pulposus cells in the intervertebral disc. Biochem Biophys Res Commun. Jul 25;372(2), 367-372 (2008)

2. Hirose, Y., Chiba, K., Karasugi, T., Nakajima, M., Kawaguchi, Y., Mikami, Y., Furuichi, T., Mio, F., Miyake, A., Miyamoto, T., Ozaki, K., Takahashi, A., Mizuta, H., Kubo, T., Kimura, T., Tanaka, T., Toyama, Y., Ikegawa, S. A Functional Polymorphism in THBS2 that Affects Alternative Splicing and MMP Binding Is Associated with Lumbar-Disc Herniation. Am J Hum Genet. May;82(5), 1122-1129 (2008)

3. Horiuchi, K., Kimura, T., Miyamoto, T., Miyamoto, K., Akiyama, H., Takaishi, H., Morioka, H., Nakamura, T., Okada, Y., Blobel, CP., Toyama, Y. Conditional Inactivation of TACE by a Sox9 Promoter Leads to Osteoporosis and Increased Granulopoiesis via Dysregulation of IL-17 and G-CSF J Immunol. 184(2), 2093-2101 (2009)

4. Iwasaki, R., Ninomiya, K., Miyamoto, K., Suzuki, T., Sato, Y., Kawana, H., Nakagawa, T., Suda, T., *Miyamoto, T. Cell fusion in osteoclasts plays a critical role in controlling bone mass and osteoblastic activity. Biochem Biophys Res Commun. 377:899-904 (2008)

5. *Miyamoto T. DC-STAMP and Osteoimmunology. Inflammation and Regeneration 29 (1), 59-62 (2009)

6. Miyamoto, K., *Miyamoto, T., Kato, R., Yoshimura, A., Motoyama, N., Suda, T. FoxO3a regulates hematopoietic homeostasis through a negative feedback pathway in conditions of stress or aging. Blood. 112(12), 4485-4493 (2008)

7. Sawatani, Y., *Miyamoto, T., Nagai, S., Maruya, M., Imai, J., Miyamoto, K., Fujita, N., Ninomiya, K., Suzuki, T., Iwasaki, R., Toyama, Y., Shinohara, M., Koyasu, S., Suda, T. The role of DC-STAMP in maintenance of immune tolerance through regulation of dendritic cell function. Int Immunol. Oct;20(10), 1259-1268 (2008)

(2009 年度 )1. Miyamoto K, Ninomiya K, Sonoda K, Miyauchi Y, Hoshi H, Iwasaki R,

Miyamoto H, Yoshida S, Sato Y, Morioka H, Chiba K, Egarashi K, Suda T, Toyama Y, *Miyamoto T. MCP-1 expressed by osteoclasts stimulates osteoclastogenesis in an autocrine/paracrine manner. Biochem Biophys Res Commun. 2009;383(3):373-377.

2. Sakai S, Takaishi H, Matsuzaki K, Kaneko H, Furukawa M, Miyauchi Y, Shiraishi A, Saito K, Tanaka A, Taniguchi T, Suda T, *Miyamoto T, Toyama Y. 1-Alpha, 25-dihydroxy vitamin D3 inhibits osteoclastogenesis through IFN-beta-dependent NFATc1 suppression. J Bone Miner Metab. 2009;27(6):643-652.

(2010 年度 )1. Shimizu T, Ishikawa T, Sugihara E, Kuninaka S, Miyamoto T, Mabuchi Y,

Matsuzaki Y, Tsunoda T, Miya F, Morioka H, Nakayama R, Kobayashi E, Toyama Y, Kawai A, Ichikawa H, Hasegawa T, Okada S, Ito T, Ikeda Y, Suda T, Saya H. c-MYC overexpression with loss of Ink4a/Arf transforms bone marrow stromal cells into osteosarcoma accompanied by loss of adipogenesis. Oncogene. 29(42):5687-5699. 2010.

2. Miyauchi Y, Ninomiya K, Miyamoto H, Sakamoto A, Iwasaki R, Hoshi H, Miyamoto K, Hao W, Yoshida S, Morioka H, Chiba K, Kato S, Tokuhisa T,

Saitou M, Toyama Y, Suda T, Miyamoto T. The Blimp1-Bcl6 axis is critical to regulate osteoclast differentiation and bone homeostasis. J Exp Med. 207(4):751-762. 2010.

（2011 年度）1. Mirza R, Qiao S, Tateyama K, Miyamoto T, Xiuli L, Seo H. 3 β

-Hydroxysterol-Delta24 reductase plays an important role in long bone growth by protecting chondrocytes from reactive oxygen species. J Bone Miner Metab. In press

2. Cui G, Watanabe K, Miyauchi Y, Hosogane N, Tsuji T, Ishii K, Nakamura M, Toyama Y, Chiba K, Miyamoto T, Matsumoto M. Matrix metalloproteinase 13 in the ligamentum flavum from lumbar spinal canal stenosis patients with and without diabetes mellitus. J Orthop Sci. In press

3. Mori T, Miyamoto T, Yoshida H, Asakawa M, Kawasumi M, Kobayashi T, Morioka H, Chiba K, Toyama Y and Yoshimura A. IL-1β and TNFα-initiated IL-6-STAT3 pathway is critical in mediating inflammatory cytokines and RANKL expression in inflammatory arthritis. Int Immunol. In press

工藤　純

(2007 年度 )1. Asai, S., Yamaki, A., Kudoh, J., Shimizu, N. & Shimizu, Y. Analysis of

the promoter region of human placenta-specific DSCR4 gene. Biochim. Biophys. Acta. 1779(1), 40-50 (2008).

2. Nagafuchi, S., Umene, K., Yamanaka, F., Oohashi, S., Shindo, M., Kurisaki, H., Kudoh, J., Shimizu, N., Hara, T. & Harada, M.S. Recurrent herpes simplex virus infection in a patient with autoimmune polyendocrinopathy–candidiasis–ectodermal dystrophy associated with L29P and IVS9-1G>C compound heterozygous autoimmune regulator gene mutations. J. Intern. Med. 261(6), 605-10 (2007).

(2008 年度 )1. Sasaki, T., Kudoh, J., Ebihara, T., Shiohama, A., Asakawa, S., Shimizu,

A., Takayanagi, A., Dekio, I., Sadahira, C., Amagai, M., Shimizu, N. Sequence analysis of filaggrin gene by novel shotgun method in Japanese atopic dermatitis. J Dermatol Sci 51(2) 113-120 (2008).

(2009 年度 )1. Fallon, P G., Sasaki, T., Sandilands, A., Campbell, L E., Saunders, S

P., Mangan, N E., Callanan, J J., Kawasaki, H., Shiohama, A., Kubo, A., Sundberg, J P., Presland, R B., Fleckman, P., Shimizu, N., Kudoh, J., Irvine, A D., Amagai, M., McLean, W H I. A homozygous frameshift mutation in the mouse Flg gene facilitates enhanced percutaneous allergen priming. Nature Genet. 41(5):602-608 (2009).

(2010 年度 )1. Fujita, H., Torii, C., Kosaki, R., Yamaguchi, S., Kudoh, J., Hayashi, K.,

Takahashi, T., and Kosaki, K. Microdeletion of the Down syndrome critical region at 21q22. Am. J. Med. Genet. Part A., 152A(4):950-953 (2010).

2. Sato, T., Takayanagi, A., Nagao, K., Tomatsu, N., Fukui, T., Kawaguchi, M., Kudoh, J., Amagai, M., Yamamoto, N., and Shimizu, N. Simple PCR-based DNA microarray system to identify human pathogenic fungi in skin. J. Clin. Microbiol., 48(7):2357-2364 (2010).

3. Wada, N., Nishifuji, K., Yamada, T., Kudoh, J., Shimizu, N., Matsumoto, M., Peltonen, L., Nagafuchi, S., and Amagai, M., Aire-dependent thymic expression of desmoglein 3, the autoantigen in pemphigus vulgaris, and its role in T-cell tolerance. J. Invest. Dermatol., 131(2):410-417 (2011).

4. 佐々木貴史、塩濱愛子、工藤 純 ; イヌゲノム解析の最前線 , 実験医学28(8) 1268-1274. (2010)

(2011 年度 ) 1. Matsuki, E., Miyakawa, Y., Asakawa, S., Tsukada, Y., Yamada, T.,

Yokoyama, K., Kudoh, J., Ikeda, Y., Okamoto, S., Identification of loss of p16 expression and upregulation of MDR-1 as genetic events resulting from two novel chromosomal translocations found in a plasmablastic lymphoma of the uterus. Clin. Cancer Res., 17(8):2101-2109 (2011).

2. Matsui, T., Miyamoto, K., Kubo, A., Kawasaki, H., Ebihara, T., Hata, K., Tanahashi, S., Ichinose, S., Imoto, I., Inazawa, J., Kudoh, J., Amagai, M.,

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SASPase regulates stratum corneum hydration through profilaggrin-to-filaggrin processing. EMBO Mol. Med., 3(6):320-333 (2011).

3. Yamaguchi, Y., Takayanagi, A., Chen, J., Sakai, K., Kudoh, J., Shimizu, N. Mouse thymic epithelial cell lines expressing "Aire" and peripheral tissue-specific antigens reproduce in vitro negative selection of T cells. Exp. Cell Res., 317(14):2019-2030 (2011).

4. 小崎健次郎、工藤 純、次世代シークエンサーを用いた疾患原因遺伝子の探索～倫理的な配慮も含めて～、細胞工学 、30(8):806-807 (2011).

伊藤　裕

(2007 年度 )1. Hasegawa, K., Wakino, S., Tatematsu, S., Yoshioka, K., Homma, K.,

Sugano, N., Kimoto, M. & Hayashi, K. & Itoh, H. Role of asymmetric dimethylarginine in vascular injury in transgenic mice overexpressing dimethylarginie, dimethylaminohydrolase 2. Circ.Res. : e2-e10, 2007

2. Ichihara, A., Itoh, H. & Inagami, T. Critical roles of (pro)rennin receptor-bound prorenin in diabetes and hypertension: sallies into therapeutic approach. Journal of the American Society of Hypertension. 2(1) (2008): 15-19, 2007.

3. Ishiguro, K., Sasamura, H., Sakamaki, Y., Itoh, H. & Saruta, T. Developmental Activity of the Renin-Angiotensin System during the “Critical Period” Modulates Later L-NAME-Induced Hypertension and Renal Injury. Hypertens Res.30(1): 63-75, 2007

4. Mitsuishi, M., Miyashita, K. & Itoh, H. cGMP rescues mitochondrial dysfunction induced by glucose and insulin in myocytes. Biochem Biophys Res Commun. 2008: 367(4): 840-5, 2008.

5. Okubo, Y., Shimada, A., Kanazawa, Y., Shigihara, T., Oikawa, Y., Imai, T., Miyazaki, J. & Itoh, H. Hyperplastic islets observed in “reversed” NOD mice treated without hematopoietic cells. Diab.Res.Clin.Pract. 79, 18-23, 2007.

6. Sakoda, M., Ichihara, A., Kaneshiro, Y., Takemitsu, T., Nakazato, Y., Nurun Nabi, A.H.M., Nakagawa, T., Suzuki, F., Inagami, T. & Itoh, H. (Pro)Renin Receptor-Mediated Activation of Mitogen-Activated ProteinKinases in Human Vascular Smooth Muscle Cells. Hypertension Res 2007; 30: 1139-1146, June 25, 2007.

7. Satofuka, S., Ichihara, A., Nagai, N., Koto, T., Shinoda, H., Noda, K., Ozawa, Y., Inoue, M., Tsubota, K., Itoh, H., Oike Y. & Ishida, S. Role of nonproteolytically activated prorenin in pathologic, but not physiologic, retinal neovascularization. Invest.Ophthalmol.Vis.Sci. 48: 422-429, 2007.

8. Takahashi, H., Ichihara, A., Kaneshiro, Y., Inomata, K., Sakoda, M., Takemitsu, T., Nishiyama, A. & Itoh, H. Regression of Nephropathy Developed in Diabetes by (Pro)rennin Receptor Blockade. J.Am.Soc.Nephrol. 18: 2054-2061, 2007

9. Yokota, K., Shibata, H., Kurihara, I., Kobayashi, S., Suda, N., Murai-Takeda, A., Saito, I., Kitagawa, H., Kato, S., Saruta, T. & Itoh, H. Coactivation of the N-terminal transactivation of mineralocorticoid receptor by Ubc9. J.Biol.Chem. 282: 1998-2010, 2007.

10. Yoshino, J., Monkawa, T., Tsuji, M., Inukai, M., Itoh, H. & Hayashi, M. Snaill is involved in the renal epithelial-mesenchymal transition. Biochem.Biophys.Res.Commun. 362(1) :63-8, Oct 12, 2007.

(2008 年度 )1. Hasegawa, K., Wakino, S., Yoshioka, K., Tatematsu, S., Hara, Y.,

Minakuchi, H., Washida, N., Tokuyama, H., Hayashi, K., Itoh, H. Sirt1 protects against oxidative stress-induced renal tubular cell apoptosis by the bidirectional regulation of catalase expression. Biochem Biophys Res Commun.372(1):51-6 (2008) Epub 2008 May 15

2. Ichihara, A., Sakoda, M., Kurauchi-Mito, A., Kaneshiro, Y., Itoh, H. Involvement of (pro)renin receptor in the glomerular filtration barrier. J Mol Med. 86(6):629-35 (2008) Epub 2008 Mar 12

3. Ishiguro, K., Hayashi, K., Sasamura, H., Sakamaki, Y., Itoh, H. "Pulse" treatment with high-dose angiotensin blocker reverses renal arteriolar hypertrophy and regresses hypertension. Hypertension. Jan;53(1):83-9 (2009)

4. Ishiguro, K., Sasamura, H., Sakamaki, Y., Hayashi, K., Saruta, T., Itoh, H. Differential effects of transient treatment of spontaneously hypertensive rats with various antihypertensive agents on the subsequent development of diabetic nephropathy. Nephron Exp Nephrol. 109(1):e20-8 (2008)

Epub 2008 May 285. Oyamada, N., Itoh, H., Sone, M., Yamahara, K., Miyashita, K., Kwijun,

P., Taura, D., Inuzuka, M., Sonoyama, T., Tsujimoto, H., Fukunaga, Y., Tamura, N., Nakao, K. Transplantation of vascular cells derived from human embryonic stem cells contributes to vascular regeneration after stroke in mice. J Transl Med. 6(1):54. 【Epub ahead of print】 2008 Sep 30

6. Oyamada, N., Sone, M., Miyashita, K., Park, K.,Taura, D., Inuzuka, M., Sonoyama,T., Tsujimoto, H., Fukunaga, Y., Tamura, N., Itoh, H., Nakao, K. The role of mineralocorticoid receptor expression in brain remodeling after cerebral ischemia. Endocrinology. 149(8): 3764-77 (2008) Epub 2008 Apr 24

(2009 年度 )1. Endo J, Sano M, Katayama T, Hishiki T, Shinmura K, Morizane S,

Matsuhashi T, Katsumata Y, Zhang Y, Ito H, Nagahata Y, Marchitti S, Nishimaki K, Wolf AM, Nakanishi H, Hattori F, Vasiliou V, Adachi T, Ohsawa I, Taguchi R, Hirabayashi Y, Ohta S, Suematsu M, Ogawa S, Fukuda K. Metabolic Remodeling Induced by Mitochondrial Aldehyde Stress Stimulates Tolerance to Oxidative Stress in the Heart. Circ Res. 2009; 105(11):1118-27

2. Hasegawa K, Wakino S, Yoshioka K, Tatematsu S, Hara Y, Minakuchi H, Sueyasu K, Washida N, Tokuyama H, Tzukerman M, Skorecki K, Hayashi K, Itoh H Kidney-specific overexpression of Sirt1 protects against acute kidney injury by retaining peroxisome function.J Biol Chem. 2010 Apr 23;285(17):13045-56

3. Morizane R, Monkawa T, Itoh H. Differentiation of murine embryonic stem and induced pluripotent stem cells to renal lineage in vitro. Biochem Biophys Res Commun. 2009 Dec 25;390(4):1334-9

4. Miyashita K, Itoh H, Tsujimoto H, Tamura N, Fukunaga Y, Sone M, Yamahara K, Taura D, Inuzuka M, Sonoyama T, Nakao K. Natriuretic peptides/cGMP/cGMP-dependent protein kinase cascades promote muscle mitochondrial biogenesis and prevent obesity.Diabetes. 2009 Dec;58(12):2880-92

5. Mitsuishi M, Miyashita K, Muraki A, Itoh H. Angiotensin II reduces mitochondrial content in skeletal muscle and affects glycemic control. Diabetes. 2009 Mar;58(3):710-7

(2010 年度 )1. Hara Y, Wakino S, Tanabe Y, Saito M, Tokuyama H, Washida N, Tatematsu

S, Yoshioka K, Homma K, Hasegawa K, Minakuchi H, Fujimura K, Hosoya K, Hayashi K, Nakayama K, Itoh H. Rho and Rho-kinase activity in adipocytes contributes to a vicious cycle in obesity that may involve mechanical stretch. Sci Signal. 2011 Jan 25;4(157):ra3.

2. Kinouchi K, Ichihara A, Sano M, Sun-Wada GH, Wada Y, Kurauchi-Mito A, Bokuda K, Narita T, Oshima Y, Sakoda M, Tamai Y, Sato H, Fukuda K, Itoh H. The (pro)renin receptor/ATP6AP2 is essential for vacuolar H+-ATPase assembly in murine cardiomyocytes. Circ Res. 2010 Jul 9;107(1):30-4.

3. Hasegawa K, Wakino S, Yoshioka K, Tatematsu S, Hara Y, Minakuchi H, Sueyasu K, Washida N, Tokuyama H, Tzukerman M, Skorecki K, Hayashi K, Itoh H. Kidney-specific overexpression of Sirt1 protects against acute kidney injury by retaining peroxisome function. J Biol Chem. 2010 Apr 23;285(17):13045-56.

（2011 年度）1. Washida N, Wakino S, Tonozuka Y, Homma K, Tokuyama H, Hara Y,

Hasegawa K, Minakuchi H, Fujimura K, Hosoya K, Hayashi K, H Itoh. Rho-kinase inhibition ameliorates peritoneal fibrosis and angiogenesis in

a rat model of peritoneal sclerosis. Nephrol Dial Transplant. Mar. 4, 2011. (Epub ahead of print) 2. Maki T, Ihara M, Fujita Y, Nambu T, Miyashita K, Yamada M, Washida N,

Nishio K, Ito H, Harada H, Yokoi H, Arai H, H Itoh, Nakao K, Takahashi R, Tomimoto H.

Angiogenic and vasoprotective effects of adrenomedullin on prevention of congnitive decline after chronic cerebral hypoperfusion in mice.

Stroke. ; 42(4): 1122-1128: Mar. 10, 2011. 3. Suda N, Shibata H, Kurihara I, Ikeda Y, Kobayashi S, Yokota K, Murai-

Takeda A, Nkagawa K, Oya M, Murai M, Rainey WE, Saruta T, H Itoh. Endocrinology. ; 152(6): 2266-2277: Apr. 5, 2011. 4. Cao Y, Nakata M, Okamoto S, Takano E, Yada T, Minokoshi Y, Hirata Y,

Nakajima K, Iskandar K, Hayashi Y, Ogawa W, Barsh GS, Hosoda H,

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Kangawa K, H Itoh, Noda T, Kasuga M, Nakae J. PDK1-Foxo1 in agouti-related peptide neurons regulates energy

homeostasis by modulating food intake and energy expenditure. PloS One. 2011; 6(4): e18324. Epub 2011 Apr 7. 5. Watanabe M, Horai Y, Houten SM, Morimoto K, Sugizaki T, Arita E, Mataki

C, Sato H, Tanigawara Y, Schoonjans K, H Itoh, Auwerx J. Lowering Beile Acid Pool Size with a Synthetic Farnesoid X Receptor

(FXR) Agonist Induces Obesity and Diabetes through Reduced Energy Expenditure.

J Bio Chem. ; 286(30): 26913-26920: Jun. 1, 2011.6. Sonoyama T, Sone M, Miyashita K, Tamura N, Yamahara K, Park K,

Oyamada N, Taura D, Inuzuka M, Kojima K, Honda K, Fukunaga Y, Kanamoto N, Miura M, Yasoda A, Arai H, H Itoh, Nakao K.

Significance of Adrenocorticotropin Stimulation Test in the Diagnosis of an Aldosterone-Producing Adenoma.

J Clin Endocrinol Metab. 2011 Jul 13. [Epub agead of print] 7. Kinouchi K, Ichihara A, H Itoh. Functional characterization of (pro)rennin receptor in association with

V-ATPase. Front Biosci. 2011 Jun 1; 17: 3216-3223. 8. Oshima Y, Kinouchi K, Ichihara A, Sakoda M, Kurauchi-Mito A, Bokuda K,

Narita T, Kurosawa H, Ge-Hong Sun-Wada, Wada Y, Yamada T, Takemoto M, Moin A. Saleem, Susan E. Quaggin, H Itoh.

(Pro)renin receptor is essential for normal podocyte structure and function.

Journal of the American Society of Nephrology 2011 (in press).

中村雅登

(2008 年度 )1. Hiroshi Suemizu, Makoto Monnai, Yasuyuki Ohnishi, Mamoru Ito, Norikazu

Tamaoki and Masato Nakamura “Identification of a key molecular regulator of liver metastasis in human pancreatic carcinoma using a novel quantitative model of metastasis in NOD/SCID/γc

null (NOG) mice” International Journal of Oncology 31: 741-751(2007)

2. Hamada, K., Monnai, M., Kawai, K., Nishime, C., Kito, C., Miyazaki, N., Ohnishi, Y., Nakamura, M., Suemizu, H. Liver metastasis models of colon cancer for evaluation of drug efficacy using NOD/Shi-scid IL2Rgcnull (NOG) mice. International Journal of Oncology. 32: 153-159 (2008)

3. Chijiwa, T., Abe, Y., Ikoma, N., Yamazaki, H., Tsukamoto, H., Suemizu, H., Kawai, K., Wakui, M., Nishime, C., Matsumoto, H., Matsuyama, M., Mukai, M., Ueyama Y., Nakamura, M. Thrombospondin 2 inhibits metastasis of human malignant melanoma through microenvironment –modification in NOD/SCID/gcnull (NOG) mice. International Journal of Oncology 34: 5-13 (2009)

4. Nakamura, M., Suemizu, H. Novel metastasis models of human cancer in NOG mice in T. Nomura et al. (eds.) Humanized Mice. Current Topics in Microbiology and Immunology 324 by Springer-Verlag Berlin Heidelberg (2008)

5. Suemizu, H., Hasegawa, M., Kawai, K., Taniguchi, K., Monnai, M., Wakui, M., Suematsu, M., Ito, M., Peltz, G., Nakamura, M. Establishment of a humanized model of liver using NOD/Shi-scid IL2Rgnull mice. Biochem. Biophys. Res. Commun. 377, 248-252 (2008)

(2009 年度 )1. M. Kawakami, A. Hirayama, K Tsuchiya, H. Ohgawara, M. Nakamura,

and K. Umezawa: Promotion of β-cell differentiation by the alkaloid conophylline in porcine pancreatic endocrine cells. Biomedicine & Pharmacotherapy, in press.

2. Handa K, Ohmura M, Nishime C, Hishiki T, Nagahata Y, Kawai K, Suemizu H, Nakamura M, Wakui M, Kitagawa Y, Suematsu M, Tsukada K. Phosphorescence-assisted microvascular O2 measurements reveal alterations of oxygen demand in human metastatic colon cancer in the liver of superimmunodeficient NOG mice. Adv Exp Med Biol. 662, 423-429, 2010.

3. Tsuyoshi Chijiwa, Yoshiyuki Abe, Yoshimasa Inoue, Hozumi Matsumoto, Kenji Kawai, Masahiro Matsuyama, Noriyuki, Miyazaki, Hiroshi Inoue, Masaya Mukai, Yoshito Ueyama and Masato Nakamura “Cancerous, but not stromal, Thrombospondin-2 contributes prognosis in pulmonary

adenocarcinoma” Oncology Reports, 22: 279-283, 20094. Masahiro Matsuyama, Tsuyoshi Chijiwa, Yoshimasa Inoue, Yoshiyuki

Abe, Masatake Nishi, Noriyuki Miyazaki, Daisuke Furukawa, Masaya Mukai, Hiroshi Suemizu, Yasutomo Sekido, Yoshito Ueyama and Masato Nakamura “Alternative splicing variant of vascular endothelial growth factor-A is a critical prognostic factor in non-small cell lung cancer” Oncology Reports, 22: 1407-1413, 2009

5. Masahiro Matsuyama, Masatoshi Wakui, Makoto Monnai, Tomoko Mizushima, Chiyoko Nishime, Kenji Kawai, Mitsuyo Ohmura, Hiroshi Suemizu, Takako Hishiki, Makoto Suematsu, Mitsuru Murata, Tsuyoshi Chjiwa, Daisuke Furukawa, Kyoji Ogoshi, Hiroyasu Makuuchi and Masato Nakamura, “Reduced CD73 expression and its association with altered purine nucleotide metabolism in colorectal cancer cells robustly causing liver metastases” Oncology Letters 1: 431-436, 2010

(2010 年度 )1. Masami Hasegawa, Kenji Kawai , Tetsuya Mitsui, Kenji Taniguchi, Makoto

Monnai,, Masatoshi Wakui, Mamoru Ito, Makoto Suematsu, Gary Peltz, Masato Nakamura, Hiroshi Suemizu “The reconstituted ʻhumanized liverʼ in TK-NOG mice is mature and functional” Biochemical and Biophysical Research Communications 405: 405–410, 2011

（2011 年度）1. Hiroshi Yamazaki, Hiroshi Suemizu, Sho Igaya, Makiko Shimizu,

Norio Shibata, Masato Nakamura, Goutam Chowdhury, and F. Peter Guengerich “In Vivo Formation of a Glutathione Conjugate Derived from Thalidomide in Humanized uPA-NOG Mice” Chemical Research in Toxicology 24: 287–289, 2011

特許権等の知的財産権出願番号：特願 2009-008097出願日：2009 年 1 月 16 日発明の名称：ヒト肝細胞が移植されたマウス発明者名：末水洋志、中村雅登、川井健司、長谷川雅己

小崎健次郎

(2008 年度 )1. Asada, H., Yagihashi, T., Furuya, M., Kosaki, K., Takahashi, T., Yoshimura,

Y. Association between patient age at the time of surgical treatment for endometriosis and aryl hydrocarbon receptor repressor polymorphism. Fertility and Sterility, 92：1240-1242(2009)

2. Hilton, E., Johnston, J., Whalen, S., Okamoto, N., Hatsukawa, Y., Nishio, J., Kohara, H., Hirano, Y., Mizuno, S., Torii, C., Kosaki, K., Manouvrier, S., Boute, O., Perveen, R., Law, C, Moore, A., Fitzpatrick, D., Lemke, J., Fellmann, F., Debray, F G., Dastot-Le-Moal, F., Gerard, M., Martin, J., Bitoun, P., Goossens, M., Verloes, A., Schinzel, A., Bartholdi, D., Bardakjian, T., Hay, B., Jenny, K., Johnston, K., Lyons, M., Belmont, J W., Biesecker, L G., Giurgea, I., Black, G. BCOR analysis in patients with OFCD and Lenz microphthalmia syndromes, mental retardation with ocular anomalies, and cardiac laterality defects. European Journal of Human Genetics. 1325-1335(2009)

3. Kosaki, R., Migita, O., Takahashi, T., Kosaki, K. Two distinctive classic genetic syndromes, 22q11.2 deletion syndrome and Angelman syndrome, occurring within the same family. American Journal of Medical Genetics, 149A:702-705 (2009)

4. Naito, Y., Kimura, T., Aramaki, M., Izumi, K., Okada, Y., Suzuki, H., Takahashi, T., Kosaki, K. Caudal regression and tracheoesophageal malformation induced by adriamycin: A novel chick model of VATER association. Pediatric Research, 65:607-612(2009)

5. Yagihashi, T., Kato, M., Izumi, K., Kosaki, R., Yago, K., Tsubota, K., Sato, Y., Okubo, M., Watanabe, G., Takahashi, T., Kosaki, K. Case report: Adult phenotype of Mulvihill-Smith syndrome. American Journal of Medical Genetics, 149A:496-500 (2009)

6. Yagihashi, T., Mizuno, M., Chino, B., Sato, Y., Sakuma, K., Takebayashi, T., Takahashi, T. Kosaki, K. Effects of the CYP2D6*10 alleles and co-medication with CYP2D6-dependent drugs on risperidone metabolism in patients with schizophrenia. Human Psychopharmacology: Clinical and Experimental, 24:301-308(2009)

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(2009 年度 )1. Hattori M, Torii C, Yagihashi T, Izumi K, Suda N, Ohyama K, Takahashi

T, Moriyama K, Kosaki K. Diagnosis of Russell-Silver syndrome by the combined bisulfite restriction analysis - Denaturing high-performance liquid chromatography assay. Genetic Testing and Molecular Biomarkers, 2009,13:623-630.

2. Fujita H, Torii C, Kosaki R, Yamaguchi S, Kudoh J, Hayashi K, Takahashi T, Kosaki K. Microdeletion of the Down syndrome critical region at 21q22. American Journal of Medical Genetics, 152:950–953.

3. Izumi K, Okuno H, Maeyama K, Sato S, Yamamoto T, Torii C, Kosaki R, Takahashi T, Kosaki K. Interstitial microdeletion of 4p16.3: Contribution of WHSC1 haploinsufficiency to the pathogenesis of developmental delay in Wolf-Hirschhorn syndrome. American Journal of Medical Genetics, 2010, 152: 1028–1032.

4. Ibrahim OM, Takefumi Y, Dogru M, Negishi K, Kosaki K, Tsubota K. Ocular complications in Mulvihill-Smith syndrome. Eye (Lond)., 24:1123-1124(2010)

5. Yamada K, Fukushi D, Ono T, Kondo Y, Kimura R, Nomura N, Kosaki K, Yamada Y, Mizuno S, Wakamatsu N. Characterization of a de novo balanced t(4;20)(q33;q12) translocation in a patient with mental retardation. American Journal of Medical Genetics, 152A:3057-3067(2010)

6. Fujita H, Yanagi T, Kosaki R, Torii C, Bamba M, Takahashi T, Kosaki K. Transverse limb defect in a patient with Jacobsen syndrome: Concurrence of malformation and disruption. American Journal of Medical Genetics, 2010, 152A:1033-1035

7. Asai S, Tanaka M, Miyakoshi K, Kim SH, Minegishi K, Matsuzaki Y, Kosaki K, Ogata H, Yoshimura Y. A case of Tessier number 7 cleft with severe micrognathia: prenatal sonographic and three-dimensional helical computed tomographic images. Prenatal Diagnosis, 2010, 30:159-161.

8. Inoue H, Takada H, Kusuda T, Goto T, Ochiai M, Kinjo T, Muneuchi J, Takahata Y, Takahashi N, Morio T, Kosaki K, Hara T. Successful cord blood transplantation for a CHARGE syndrome with CHD7 mutation showing DiGeorge sequence including hypoparathyroidism. European Journal of Pediatrics,169:839-844(2010)

9. Hosokawa S, Takahashi N, Kitajima H, Nakayama M, Kosaki K, Okamoto N. A case of Brachmann-de Lange syndrome with congenital diaphragmatic hernia and NIPBL gene mutation. Congenit Anom, 50:129-132(2010)

(2010 年度 )1. Fujita H, Torii C, Kosaki R, Yamaguchi S, Kudoh J, Hayashi K, Takahashi

T, Kosaki K. Microdeletion of the Down syndrome critical region at 21q22. American Journal of Medical Genetics, 2010; 152:950–953.

2. Izumi K, Okuno H, Maeyama K, Sato S, Yamamoto T, Torii C, Kosaki R, Takahashi T, Kosaki K. Interstitial microdeletion of 4p16.3: Contribution of WHSC1 haploinsufficiency to the pathogenesis of developmental delay in Wolf-Hirschhorn syndrome. American Journal of Medical Genetics, 2010; 152: 1028–1032.

3. Fujita H, Yanagi T, Kosaki R, Torii C, Bamba M, Takahashi T, Kosaki K. Transverse limb defect in a patient with Jacobsen syndrome: Concurrence of malformation and disruption. American Journal of Medical Genetics, 2010; 152A:1033-1035.

4. Kosaki R, Kikuchi S, Koinuma G, Higuchi M, Torii C, Kawasaki K, Kosaki K.Two patients with Rubinstein-Taybi syndrome and severe pulmonary interstitial involvement. American Journal of Medical Genetics, 2010; 152:1844-1846.

5. Yagihashi T, Hatori K, Ishii K, Torii C, Momoshima S, Takahashi T, Kosaki K. Juvenile muscular atrophy of a unilateral upper extremity (Hirayama disease) in a patient with CHARGE syndrome, Molecular Syndromology, 2010; 1:91-94.

6. Ohnuki Y, Torii C, Kosaki R, Yagihashi T, Sago H, Yasukawa K, Takahashi T, Kosaki K. Cri-du-chat syndrome cytogenetically cryptic recombination aneusomy of chromosome 5: Implications in recurrence risk estimation. Molecular Syndromology, 2010;1:95-98.

7. Izumi K, Takagi M, Parikh A, Hahn A, Miskovsky S, Nishimura G, Torii C, Kosaki K, Hasegawa T, Neilson DE.

Late manifestations of Tricho-Rhino-Pharangeal syndrome in a patient: Expanded skeletal phenotype in adulthood. American Journal of Medical Genetics, 2010; 152:2115-2119.

8. Fukushima H, Kosaki K, Sato R, Yagihashi T, Gatayama R, Kodo K, Hayashi T, Nakazawa M, Tsuchihashi T, Maeda J, Kojima Y, Yamagishi H, Takahashi T. Mechanisms underlying early development of pulmonary vascular obstructive disease in Down syndrome: An imbalance in biosynthesis of thromboxane A2 and prostacyclin. American Journal of Medical Genetics, 2010;152:1919-1924.

9. Yoshihashi H, Ohki H, Torii C, Ishiko A, Kosaki K. Survival of a male mosaic for PORCN mutation with mild focal dermal

hypoplasia phenotype. Pediatric Dermatology, 28:550-554(2011)10. Kosaki R, Fujita H, Ueoka K, Torii C Kosaki K. Overgrowth of prenatal onset associated with submicroscopic 9q22.3

deletion. American Journal of Medical Genetics, 155:903-905(2011)11. Kosaki R, Fujita H, Takada H, Okada M, Torii C, Kosaki K. Monozygotic

twins of Rubinstein-Taybi Syndrome discordant for glaucoma. American Journal of Medical Genetics, 155:1189-1191(2011)

12. Yanagimachi M, Naruto T, Tanoshima R, Kato H, Yokosuka T, Kajiwara R, Fujii H, Tanaka F, Goto H, Yagihashi T, Kosaki K, Yokota S. Influence of CYP3A5 and ABCB1 gene polymorphisms on calcineurin inhibitor-related neurotoxicity after hematopoietic stem cell transplantation. Clin Transplant 2010; 24:855-861

（2011 年度）1. Kosaki K. Role of rare cases in deciphering the mechanisms of

congenital anomalies: CHARGE syndrome research. Congenit Anom 2011; 51:12-15.

2. Watanabe K, Okada E, Kosaki K, Tsuji T, Ishii K, Nakamura M, Chiba K, Toyama Y, Matsumoto M.Surgical treatment for scoliosis in patients with Shprintzen-Goldberg syndrome. J Pediatr Orthop 2011; 31:186-193.

3. Honda H, Takubo K, Oda H, Kosaki K, Tazaki T, Yamasaki N, Miyazaki K, Moore KA, Honda Z, Suda T, Lemischka IR. Hemp, an mbt domain-containing protein, plays essential roles in hematopoietic stem cell function and skeletal formation. Proc Natl Acad Sci U S A 2011; 108:2468-2473.

4. Suda N, Hattori M, Kosaki K, Banshodani A, Kozai K, Tanimoto K, Moriyama K　Correlation between genotype and supernumerary tooth formation in cleidocranial dysplasia. Orthod Craniofac Res 2010; 13:197-202.

5. Numabe H, Sawai H, Yamagata Z, Muto K, Kosaki R, Yuki K, Kosaki K. Reproductive success in patients with Hallermann-Streiff syndrome. American Journal of Medical Genetics，2011;155A：2311-2313

6. Kosaki K, Saito H, Kosaki R, Torii C, Kishi K, Takahashi T. Branchial arch defects and 19p13.12 microdeletion: Defining the critical region into a 0.8M base interval. American Journal of Medical Genetics,2011;155A:2212-2214

7. Sasaki K, Okamoto N, Kosaki K, Yorifuji T, Shimokawa O, Mishima H, Yoshiura KI, Harada N. Maternal uniparental isodisomy and heterodisomy on chromosome 6 encompassing a CUL7 gene mutation causing 3M syndrome. Clin Genet (in press)

佐々木えりか

(2008 年度 )1. Kohu, K., Yamabe, E., Mstsuzawa, A., Onda, D., Suemizu, H., Sasaki,

E., Tanioka, Y., Yagita, H., Suzuki, D., Kametani, Y., Takai, T., Toyoda, A., Habu, S., Satake, M. Comparison of 30 Immunity-Related Genes from the Common Marmoset with Orthologues from Human and Mouse. Tohoku J. Exp. Med., 215: 167-180 (2008)

2. Tomioka, I., Sasaki, E. Recent progress in reproductive technologies based on the common marmoset (Callithrix jacchus). J. Mamm. Ova Res., 25, 143-149 (2008)

3. Hao Chen, Hattori, F., Murata, M., Li, W., Yuasa, S., Onitsuka, T., Shimoji, K., Ohno, Y., Sasaki, E., Kimura, K., Hakuno, D., Sano, M., Makino, S., Ogawa, S., and Fukuda, K. Common marmoset embryonic stem cell can differentiate into cardiomyocytes. BBRC, 2008 May 9;369(3):801-6

(2009 年度 )1. Hattori F, Chen H, Yamashita H, Tohyama S, Satoh Y, Yuasa S, Li W,

Yamakawa H, Tanaka T, Onitsuka T, Shimoji K, Ohno Y, Egashira T, Kaneda R, Murata M, Hidaka K, Morisaki T, Sasaki E, Suzuki T, Sano M,

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業績集 L i s t o f A c h i e v e m e n t s113

Makino S, Oikawa S, Fukuda K. Nongenetic method for purifying stem cell–derived cardiomyocytes. Nature Methods. 2010; 7(1):61-6

2. Sotomaru, Y ., Hirakawa, R., Shimada, A., Shiozawa, S., Sugawara, A., Oiwa, R., Nobukiyo, A., Okano, H., Tamaoki, N., Nomura, T., Hiyama, E., Sasaki, E. Pre-implantation Development of Somatic Cell Cloned Embryos in the Common Marmoset (Callithrix jacchus). Cloning and Stem cells, Vol. 11, No.4, 575-583, December, 2009

3. Kametani, Y., Suzuki, D., Kohu, K., Satake, M., Suemizu, H., Sasaki, E., Ito, T., Tamaoki, N., Mizushima, T., Ozawa, M., Tani, K., Kito, M., Arai, H., Koyanagi, A., Yagita, H., Habu. S. Development of Monoclonal Antibodies for Analyzing Immune and Hematopoietic Systems of Common Marmoset. Exp Hematol., Vol. 37, No.11, 1318-1329, November, 2009

4. Koso, H., Minami, C., Tabata, Y., Inoue, M., Sasaki, E., Satoh, S., Watanabe, S. CD73 is a novel cell surface antigen characterizes retinal photoreceptor precursor cells. Invest Ophthalmol Vis Sci., Vol. 50, No, 11, 5411-5428, November, 2009

5. Fleischmann, G., Müller, T., Behr, R., Blasczyk, R., Sasaki, E., Horn, P. A. Growth characteristics of the non-human primate embryonic stem cell line cjes001 depending on feeder cell treatment. Cloning and Stem cells, Vol.11, No.2, 225-33, June, 2009

6. Müller, T., Fleischmann, G., Eildermann, K., Mätz-Rensing, K., Horn, PA., Sasaki, E., Behr, R. A novel embryonic stem cell line derived from the common marmoset monkey (Callithrix jacchus) exhibiting germ cell-like characteristics. Hum. Repr., Vol. 24, No. 6, 1359-1372, June, 2009

7. Sasaki, E., Suemizu, H., Shimada, A., Hanazawa, K., Oiwa, R., Kamioka, M., Tomioka, I., Sotomaru, Y., Hirakawa, R., Eto, T., Shiozawa, S., Maeda, T., Ito, M., Ito, R., Kito, C., Yagihashi, C., Kawai, K., Miyoshi, H., Tanioka, Y., Tamaoki, N., Habu, S., Okano, H., Nomura, T. Generation of transgenic nonhuman primates with germline transmission. Nature, Vol.28, No.459, 523-527, May, 2009

(2010 年度 )1. Tomioka, I., Maeda, T., Shimada, H., Kawai, K., Okada, Y., Igarashi,

H., Oiwa, R., Iwasaki, T., Aoki, M., Kimura, T., Shiozawa, S., Shinohara, H., Suemizu, H., Sasaki, E*., Okano, H. (2010) Generating induced pluripotent stem cells from common marmoset (Callithrix jacchus) fetal liver cells using defined factors, including Lin28. Genes Cells. 15:959-69.(* corresponding author)

2. Li W, Yamashita, H., Hattori, F., Chen H, Tohyama S, Satoh Y, Sasaki E, Yuasa S, Makino S, Sano M, Fukuda K. (2011) Simple autogeneic feeder cell preparation for pluripotent stem cells. Stem Cell Res.; 6:83-9.

3. Hikishima, K., Quallo, MM., Komaki, Y., Yamada M., Kawai K., Momoshima, S., Okano HJ., Sasaki, E., Tamoki, N., Lemon RN., Iriki, A., Okano, H. (2011) Population-averaged standard template brain atlas for the common marmoset (Callithrix jacchus). Neuroimage.; 54:2741-9.

4. Shiozawa, S., Kawai, K., Okada, Y., Tomioka, I., Maeda, T., Kanda, A., Shinohara, H., Suemizu, H., Okano, H.J., Sotomaru, Y., Sasaki, E., Okano, H. (2011) Gene targeting and subsequent site-specific transgenesis at the β -actin (ACTB) locus in common marmoset embryonic stem cells. Stem Cells Dev. 2011 September 20(9): 1587-1599.

5. 佐々木えりか、遺伝子改変霊長類作出法の確立 レンチウィルスベクターによる遺伝子導入法でトランスジェニックマー

モセットを作出　化学と生物 　Vol.48 No.6, 2010　559　p372-3746. 佐々木えりか、ちょっと変な動物，コモンマーモセット 細胞工学 　2010 年 8 月号 (2010 年 07 月 22 日発行 )

(2011 年度 )1. Tanaka, DH., Oiwa, R., Sasaki, E., Nakajima, K. Changes in cortical

interneuron migration contribute to the evolution of the neocortex. Proc Natl Acad Sci USA. 2011 May 10;108(19):8015-20.

特許権等の知的財産権1. 卵採取及び胚移植用器具 出願番号：特願 2010-279684 出願日：平成 22 年 12 月 15 日

佐野元昭

(2007 年度 )1. Sano M, Izumi Y, Helenius K, Asakura M, Rossi DJ, Xie M, Taffet G, Hu

L, Pautler RG, Wilson CR, Boudina S, Abel ED, Taegtmeyer H, Scaglia F, Graham BH, Kralli A, Shimizu N, Tanaka H, Makela TP, Schneider MD. Ménage-à-trois 1 is indisoensable for the transcriptional function of PPARγ coactivatior-1. Cell Metabolism 5:129-42. (2007)

2. Liu Y, Asakura M, Inoue H, Nakamura T, Sano M, Niu Z, Chen M, Schwartz RJ, Schneider MD. Sox17 is essential for the specification of cardiac mesoderm in embryonic stem cells. Proc Natl Acad Sci U S A. 104 :3859-64. (2007)

3. Ieda M, kanazawa H, Kimura K, Hattori F, Ieda Y, Taniguchi M, Lee JK, Matsumura K, Tomita Y, Miyoshi S, Shimoda K, Makino S, Sano M, Kodama I, Ogawa S, Fukuda K. Sama3a maintains normal heart rhythm through sympatic innervation patterning. Nat Med 13: 604-612. (2007)

4. Endo J, Sano M, Fujita J, Hayashida K, Yuasa S, Aoyama N, Takehara Y, Kato O, Makino S, Ogawa S, Fukuda K. Bone Marrow Derived Cells Are Involved in the Pathogenesis of Cardiac Hypertrophy in Response to Pressure Overload. Circulation. (2007) Aug 13

5. Sano M, Tokudome S, Shimizu N, Yoshikawa N, Ogawa C, Shirakawa K, Endo J, Katayama T, Yuasa S, Ieda M, Makino S, Hattori F, Tanaka H, Fukuda K. Intramolecular Control of Protein Stability, Subnuclear Compartmentalization, and Coactivator Function of Peroxisome Proliferator-activated Receptor γCoactivator 1α. J Biol Chem. 282(35):25970-80.(2007)

(2008 年度 )1. Hayashida K, Sano M, Ohsawa I, Shinmura K, Tamaki K, Kimura K, Endo

J, Katayama T, Kawamura A, Kohsaka S, Makino S, Ohta S, Ogawa S, Fukuda K. Inhalation of hydrogen gas reduces infarct size in the rat model of myocardial ischemia-reperfusion injury. Biochem Biophys Res Commun. (2008);373(1):30-5.

2. Yoshikawa N, Shimizu N, Sano M, Ohnuma K, Iwata S, Hosono O, Fukuda K, Morimoto C, Tanaka H. Role of the hinge region of glucocorticoid receptor for HEXIM1-mediated transcriptional repression. Biochem Biophys Res Commun. (2008);371(1):44-9.

3. Shimizu, N., Yoshikawa, N. Wada, T., Handa, H., Sano, M., Fukuda, K., Suematsu, M., Sawai, T., Morimoto, C., Tanaka, H. Tissue- and context-dependent modulation of hormonal sensitivity of glucocorticoid-sensitive genes by HEXIM1. Mol. Endocrinol. 22, 2609-2623 (2008)

4. Tokudome S, Sano M, Shinmura K, Matsuhashi T, Morizane S, Moriyama H, Tamaki K, Hayashida K, Nakanishi H, Yoshikawa N, Shimizu N, Endo J, Katayama T, MurataM, Yuasa S, Kaneda R, Tomita K, Eguchi N, Urade Y, Asano K, Utsunomiya Y, Suzuki T, Taguchi R, Tanaka H, Fukuda K. Glucocorticoid protects heart from ischemia-reperfusion injury through activation of lipocalin-type prostaglandin D synthase-derived PGD2 biosynthesis. J Clin Invest. 2009

5. Yoshikawa N, Nagasaki M, Sano M, Tokudome S, Ueno K, Shimizu N, Imoto S, Miyano S, Suematsu M, Fukuda K, Morimoto C, Tanaka H. Ligand-based gene expression profiling reveals novel roles of glucocorticoid receptor in cardiac metabolism. Am J Physiol Endocrinol Metab. 2009 Mar 17.

6. Sano M, Fukuda K. Mitochondrial biogenesis by hormesis. Circ Res. 103:1191-3 (2008)

(2009 年度 )1. Yoshikawa N, Nagasaki M, Sano M, Tokudome S, Ueno K, Shimizu

N, Imoto S, Miyano S, Suematsu M, Fukuda K, Morimoto C, Tanaka H. Ligand-based gene expression profiling reveals novel roles of glucocorticoid receptor in cardiac metabolism. Am J Physiol Endocrinol Metab 296, E1363-E1373, 2009.

2. Endo J, Sano M, Katayama T, Hishiki T, Shinmura K, Morizane S, Matsuhashi T, Katsumata Y, Zhang Y, Ito H, Nagahata Y, Marchitti S, Nishimaki K, Wolf AM, Nakanishi H, Hattori F, Vasiliou V, Adachi T, Ohsawa I, Taguchi R, Hirabayashi Y, Ohta S, Suematsu M, Ogawa S, Fukuda K. Metabolic remodeling induced by mitochondrial aldehyde stress stimulates tolerance to oxidative stress in the heart. Circ Res 105(11), 1118-27, 2009.

3. Katayama T, Sano M, Endo J, Hishiki T, Nagahata Y, Tokudome S,

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114

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List of Achievem

ents

Shinmura K, Nishimaki K, Matsuhashi T, Katsumata Y, Morizane S, Ito H, Takehana K, Cavener DR, Ohsawa I, Ohta S, Suematsu M, Fukuda K. Intracellular depletion of histidine mediates stress response to aldehydes. Circ Res 105(11), 1118-1127, 2009.

4. Tokudome S, Sano M, Shinmura K, Matsuhashi T, Morizane S, Moriyama H, Tamaki K, Hayashida K, Nakanishi H, Yoshikawa N, Shimizu N, Endo J, Katayama T, MurataM, Yuasa S, Kaneda R, Tomita K, Eguchi N, Urade Y, Asano K, Utsunomiya Y, Suzuki T, Taguchi R, Tanaka H, Fukuda K. Glucocorticoid protects heart from ischemia-reperfusion injury through activation of lipocalin-type　 prostaglandin D synthase-derived PGD2 biosynthesis. J Clin Invest. 2009;119(6):1477-88.

5. Yoshikawa N, Nagasaki M, Sano M, Tokudome S, Ueno K, Shimizu N, Imoto S, Miyano S, Suematsu M, Fukuda K, Morimoto C, Tanaka H. Ligand-based gene expression profiling reveals novel roles of glucocorticoid receptor in cardiac metabolism. Am J Physiol Endocrinol Metab. 2009 296(6):E1363-73.

6. Suzuki Y, Sano M, Hayashida K, Ohsawa I, Ohta S, Fukuda K. Are the effects of alpha-glucosidase inhibitors on cardiovascular events related to elevated levels of hydrogen gas in the gastrointestinal tract? FEBS Lett. 2009; 583 (13):2157-9.

7. Endo J, Sano M, Katayama T, Hishiki T, Shinmura K, Morizane S, Matsuhashi T, Katsumata Y, Zhang Y, Ito H, Nagahata Y, Marchitti S, Nishimaki K, Wolf AM, Nakanishi H, Hattori F, Vasiliou V, Adachi T, Ohsawa I, Taguchi R, Hirabayashi Y, Ohta S, Suematsu M, Ogawa S, Fukuda K. Metabolic Remodeling Induced by Mitochondrial Aldehyde Stress Stimulates Tolerance to Oxidative Stress in the Heart. Circ Res. 2009; 105(11):1118-27.

8. Sano M. Mitochondrial protection and the reversal of left ventricular remodeling. Circ J. 2009 73(11):2017-8.

9. Hattori F, Chen H, Yamashita H, Tohyama S, Satoh Y, Yuasa S, Li W, Yamakawa H, Tanaka T, Onitsuka T, Shimoji K, Ohno Y, Egashira T, Kaneda R, Murata M, Hidaka K, Morisaki T, Sasaki E, Suzuki T, Sano M, Makino S, Oikawa S, Fukuda K. Nongenetic method for purifying stem cell–derived cardiomyocytes. Nature Methods. 2010; 7(1):61-6

10. Takeda N, Manabe I, Uchino Y, Eguchi K, Matsumoto S, Shindo T, Sano M, Otsu K, Snider P, Conway S, Nagai R. Cardiac fibroblasts are essential for the adaptive response of the murine heart to pressure overload. J Clin Invest. 2010; 120(1):254-65

11. Kanazawa H, Ieda M, Kimura K, Arai T, Kawaguchi-Manabe H, Matsuhashi T, Endo J, Kawakami T, Sano M, Kimura T, Monkawa T, Shimoda K, Hayashi M, Iwanami A, Okano H, Okada Y, Ishibashi-Ueda H, Ogawa S, Fukuda K. Heart failure causes cholinergic transdifferentiation of cardiac sympathetic nerves via gp130-mediated cytokines. 2010; 120(2): 408-421. J Clin Invest.

12. Sano M, Fukuda K. The selective VEGFR inhibitor PTK787/ZK 222584 represses the activities of VEGFR-negative bone marrow-derived mesenchymal stem cells. Cancer Biol Ther. 2009;8 (13):1249-51.

13. Sano M. Mitochondrial protection and the reversal of left ventricular remodeling. Circ J. 2009;73(11):2017-8.

14. 佐野元昭　菱木貴子 , 末松　誠 , 中西広樹 , 田口　良　第 1 章「循環器の生物学」メタボローム . Annual Review 循環器 2009年版 　25-28（中外医学社）

15. 佐野元昭　横尾〔徳留〕さとり . 遺伝子医学 MOOK16号 「メタボロミクスと最新分子バイオマーカー研究 」　ステロイドホルモンの心筋障害抑制作用とプロスタグランジン D2　株式会社メディカル ドゥ

16. 佐野元昭　「心不全発症のメカニズム」酸化ストレスと心筋老化　Bio Clinica 24 (10), 2009, 891-896

17. 佐野元昭 心筋ミトコンドリアの酸化的障害に対する代謝応答　医学のあゆみ 「心不全－研究と臨床の最前線」　Vol. 232 No. 58887-8891( 医歯薬出版株式会社 )

18. 佐野元昭 第 3 章 Cardio Diabetic Disease の分子機序にせまる 10. サーチュイン

19. 佐野元昭　特集 / 心臓血管の発生、疾患に関与する新しい注目すべき分子4. THYMOSIN BETA-4

20. Hattori, F., Chen, H., Yamashita, H., Tohyama, S., Satoh, Y., Yuasa, S., Li, W., Yamakawa, H., Tanaka, T., Onitsuka, T., Shimoji, K., Ohno, Y., Egashira, T., Kaneda, R., Murata, M., Hidaka, K., Morisaki, T., Sasaki, E., Suzuki, T., Sano, M., Makino, S., Oikawa, S and Fukuda, K. A non-genetic method for purifying stem cell-derived cardiomyocytes. Nature Method., Vol.7 , No.1, 61-66, January, 2010

(2010 年度 )1. Hattori F, Chen H, Yamashita H, Tohyama S, Satoh Y, Yuasa S, Li W,

Yamakawa H, Tanaka T, Onitsuka T, Shimoji K, Ohno Y, Egashira T, Kaneda R, Murata M, Hidaka K, Morisaki T, Sasaki E, Suzuki T, Sano M, Makino S, Oikawa S, Fukuda K. Nongenetic method for purifying stem cell–derived cardiomyocytes. Nature Methods. 7(1):61-6 (2010)

2. Takeda N, Manabe I, Uchino Y, Eguchi K, Matsumoto S, Shindo T, Sano M, Otsu K, Snider P, Conway S, Nagai R. Cardiac fibroblasts are essential for the adaptive response of the murine heart to pressure overload. J Clin Invest. 120(1):254-65 (2010)

3. Kanazawa H, Ieda M, Kimura K, Arai T, Kawaguchi-Manabe H, Matsuhashi T, Endo J, Kawakami T, Sano M, Kimura T, Monkawa T, Shimoda K, Hayashi M, Iwanami A, Okano H, Okada Y, Ishibashi-Ueda H, Ogawa S, Fukuda K. Heart failure causes cholinergic transdifferentiation of cardiac sympathetic nerves via gp130-mediated cytokines. J Clin Invest. 120(2): 408-421 (2010)

4. Zhang Y, Sano M, Shinmura K, Tamaki K, Katsumata Y, Matsuhashi T, Morizane S, Ito H, Hishiki T, Endo J, Zhou H, Yuasa S, Kaneda R, Suematsu M, Fukuda K. 4-Hydroxy-2-nonenal protects against cardiac ischemia-reperfusion injury via the Nrf2-dependent pathway. J Mol Cell Cardiol. 49(4):576-86 (2010) (Corresponding author, IF = 5.054)

5. Kimura K, Kanazawa H, Ieda M, Kawaguchi-Manabe H, Miyake Y, Yagi T, Arai T, Sano M, Fukuda K. Norepinephrine-induced nerve growth factor depletion causes cardiac sympathetic denervation in severe heart failure. Auton Neurosci . 156(1-2):27-35. (2010) (IF=1.815)

6. Sano M. Cardioprotection by hormetic response to aldehyde. Circ J.74(9):1787-93 (2010)

7. Yuasa S, Onizuka T, Shimoji K, Ohno Y, Kageyama T, Yoon SH, Egashira T, Seki T, Hashimoto H, Nishiyama T, Kaneda R, Murata M, Hattori F, Makino S, Sano M, Ogawa S, Prall OW, Harvey RP, Fukuda K. Zac1 is an essential transcription factor for cardiac morphogenesis. Circ Res. 106(6): 1083-91(2010)

8. Kinouchi K, Ichihara A, Sano M, Sun-Wada GH, Wada Y, Kurauchi-Mito A, Bokuda K, Narita T, Oshima Y, Sakoda M, Tamai Y, Sato H, Fukuda K, Itoh H. The (pro)renin receptor/ATP6AP2 is essential for vacuolar H+-ATPase assembly in murine cardiomyocytes. Circ Res. 107(1):30-4. (2010)

9. Tamura Y, Matsumura K, Sano M, Tabata H, Kimura K, Ieda M, Arai T, Ohno Y, Kanazawa H, Yuasa S, Kaneda R, Makino S, Nakajima K, Okano H, and Fukuda K. Neural crest-derived stem cells migrate and differentiate into cardiomyocytes after myocardial infarction. Arterioscler Thromb Vasc Biol. 31(3):582-9. (2010)

10. Crosstalk between glucocorticoid receptor and nutritional sensor mTOR in skeletal muscle. Shimizu N, Yoshikawa N, Ito. N, Maruyama T, Suzuki Y, Takeda S, Tagata Y, Nishitani S, Takehana K, Sano M, Fukuda K, Suematsu M, Morimoto C, Tanaka H. Cell Metab. 13(2):170-82.( 2011)

11. Impact of Long-term Caloric Restriction on Cardiac Senescence: Caloric Restriction Ameliorates Cardiac Diastolic Dysfunction Associated with Aging. Shinmura K, Tamaki K, Sano M, Murata M, Yamakawa H, Ishida H, Fukuda K. J Mol Cell Cardiol.;50(1):117-27.(2011)

12. G-CSF influences mouse skeletal muscle development and regeneration by stimulating myoblast proliferation. Hara M, Yuasa S, Shimoji K, Onizuka T, Hayashiji N, Ohno Y, Arai T, Hattori F, Kaneda R, Kimura K, Makino S, Sano M, Fukuda K. J Exp Med. 2011

（2011 年度）1. Sano M. Revisiting the merits of nifedipine. Hypertension Research (2011) 2. Tohyama S, Hattori F, Sano M, Hishiki T, Nagahata Y, Matsuura T,

Yamashita H, SatoH Y, Hashimoto H, Egashira T, Seki T, Muraoka N, Yamakawa H, Yuasa S, Murata M, Suematsu M, Fukuda K. Distinct metabolic flow enables large scale purification of cardiomyocytes from pluripotent stem cell-derivatives.(2011) Cell Stem Cell (Revised). (IF=26.967)

3. Sano M, Matsuhashi T, Kohsaka S, Fukuda K, Suzuki Y. Sitagliptin counteracts seasonal fluctuation of glycemic control in Japanese with type 2 diabetes.(2011) Diabetes Research and Clinical Practice

4. MeguroS, Sano M, Matsuhahi T, Suzuki Y. Good glycemic control with sitagliptin plus a flexible diet toprevent hypoglycemia in patients with type 2 diabetes. (2011) Diabetes Research and Clinical Practice

5. Morizane S, Sano M, Mitani F, Matsuhashi T, Ozawa K, Katsumata Y, Ito H, Shinmura K, Kinouchi K, Nishiyama A, Honma S, Suzuki T, Fukuda

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業績集 L i s t o f A c h i e v e m e n t s115

K. Chronic salt loading in salt-sensitive rats paradoxically increases aldosterone production.(2011) Hypertension (revised)

6. Ito H, Sano M, Nagata N, Aritake K, Katsumata Y, Xiaoxiang Y, Morizane S, Matsuhashi T, Urade Y, Asano K, Utsunomiya Y, Hosoya T, Fukuda K. Lipocalin-type prostaglandin D2 synthase promotes renal interstitial fibrosis via Th2 lymphocyte activation in unilateral ureteral obstruction. (Paper Submitted)

7. 佐野元昭、木村謙介、小平真幸　話題の心筋症　ミトコンドリア異常と心筋病変　循環器病理 。2011 年　29 巻 2 号 159-164

8. 佐野元昭、福田恵一 冠動脈疾患 ( 上 ) 心エネルギー代謝と冠循環 日本臨床 2011 年 60-68

9. 佐 野 元 昭「HEARTʼs Selection」『 心 臓 大 血 管 の 発 生 と 遺 伝 子 異 常 』ATP6ap2/(Pro)renin receptor心臓 2011 年 5 月号

10. 勝俣良紀、佐野元昭 『心臓の謎に迫る』「糖代謝異常により心機能は低下するか」Angiotensin Research, 8 巻 , 3 号 2011 年 7 月号

11. 佐野元昭、福田恵一　特集 RAS は心と人をつなぐかー心腎連関と RAS―心不全と腎臓病　Angiotensin Research 2011 年 8 巻 2 号 23-29 先端医学社

12. 佐野元昭 State of the ART 食塩感受性とアルドステロン― 副腎における組織レニン・アンジオテンシン系活性化の意義 ― 臨床高血圧 メディカルレビュー社 2011 年 6 月号

13. 鈴木吉彦、佐野元昭、目黒周、河合俊英 GLP-1 受動体作動薬は逆流性食道炎による気管支喘息の原因になりえる　月刊医学と薬学　2011 年 66 巻 3 号

14. 佐野元昭　「エネルギー代謝と生活習慣病」エネルギー代謝とメタボローム・トランスクリプト―ム解析　Bio Clonica 2011 年 11 月号

15. 鈴木吉彦、佐野元昭、河合俊英、山田悟　インクレチン関連薬の薬剤選択と使い方　メディカルトリビューン

16. 佐野元昭、福田恵一　冠動脈疾患 ( 上 )　心エネルギー代謝と冠循環　日本臨床 　2011 年　69(-) (1006), 60-68, 2011-09

17. 佐野元昭　代謝の異常は心不全の原因か　Cardiovascular Frontier 2011年　Cardiovascalar Frontier 2(3),167-173,2011-06 メディカルレビュー社

特許権等の知的財産権提案の名称：PGD2 による心筋虚血再灌流障害治療発明の名称：医薬組成物出願番号：特願 2009-047014出願日：2009 年 2 月 27 日弊所整理番号：000001644 佐野元昭、福田恵一

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