Upload
brandon-dennis
View
28
Download
4
Embed Size (px)
DESCRIPTION
Chapter 1 Molecular Biology and Biological Chemistry. 暨南大學資訊工程學系 黃光璿 (HUANG, Guan-Shieng) 2004/02/23. 常見的化學元素. 週期表. Link 1 ( 注音 ) Link 2 ( 台灣師大 ). 水: H 2 O 氧氣: O 2 氮氣: N 2 乙醇(酒精): C 2 H 5 OH. 1.1 Genetic Material. DNA: ( deoxyribonucleic acid, 去氧核醣核酸) phosphate group ( 磷酸鹽 ) - PowerPoint PPT Presentation
Citation preview
1
Chapter 1Molecular Biology and Biological Chemistry
暨南大學資訊工程學系黃光璿 (HUANG, Guan-Shieng)2004/02/23
2
常見的化學元素
3
4
週期表
Link 1 ( 注音 ) Link 2 ( 台灣師大 )
5
水: H2O 氧氣: O2
氮氣: N2
乙醇(酒精): C2 H5 OH
6
1.1 Genetic Material
DNA: ( deoxyribonucleic acid, 去氧核醣核酸) phosphate group ( 磷酸鹽 ) deoxyribose sugar nitrogenous base
A: Adenine G: GuanineC: Cytosine T: Thymine
RNA
7
1.1.1 DNA --- A, G, C, T
8
1.1.2 DNA is oriented
5’ – GTATCC – 3’3’ – GTATCC – 5’ not equal
9
10
gene (Mendel, 1860s) genome: all of an organism’s genetic
instructions
11
1.1.3 Base paring of DNA
G pairs with C and A pairs with T. reverse complement
5’- GTATCC – 3’ 3’- CATAGG – 5’
upstream: 5’ downstream: 3’
12
13
DNA 雙螺旋體( DNA double helix )
14
1.1.4 Central Dogma of Molecular Biology
15
1.1.5 RNA
RNA: ( ribonucleic acid, 核醣核酸) phosphate group ribose sugar nitrogenous base (A, G, C, U: Uracil)
NAD
16
1.2 Gene Structure and Information Content promoter sequence
17
1.2.1 Regulatory Proteins
positive regulation negative regulation
18
1.2.2 Amino Acids ( 胺基酸 )
amino
carboxyl
alpha
19
20
21
1.2.3 Genetic Code
22
4 nucleotides 20 amino acid 4x4x4=64 > 20 triplet code (codon)
This genetic code is universally used by all living things today with only a few exceptions.
23
1.2.4 Open Reading Frames
A substring in DNA that contains no stop codons (UAA, UAG, UGA) while reading in a single reading frame.
How can one detect ORFs? Long ORFs are easy, since the probability that a
stop codon appears randomly is 3/64.
24
1.2.5 Introns and Exons
eukaryotes ˇ prokaryotes × Extreme example
cystic fibrosis: 24 introns, 1K/1M nts splicing & alternative splicing
25
1.3 Protein Structure and Function Proteins
structure proteins: collagen ( 膠原 ) enzymes: pepsin ( 胃液素 ) transportation: hemoglobin ( 血紅素 ) signaling & intercellular communication: insuli
n ( 胰島素 ) absorbing photons: rhodopsin ( 視網膜色素 )
26
1.3.1 Primary Structure
amino acid, polypeptide chain amino terminus: H2N- ( 胺基 ) carboxyl terminus: -COOH ( 羧基 )
27
28
29
蛋白質是由 20 種胺基酸所組成
30
31
32
1.3.2 Secondary Structure
α-helix and β-sheet
33
34
1.3.3 Tertiary and Quaternary Structure
35
1.4 The Nature of Chemical Bonds anatomy of an atom
neutron ( 中子 ) proton ( 質子 ) + electron ( 電子 ) –potential energy
valence ( 原子價 ) covalent bond ( 共價鍵 )
36
electronegativity polar bond, hydrogen bond
hydrophilicity ( 親水性 ) and hydrophobicity ( 疏水性 )
37
1.5 Molecular Biology Tools
38
1.5.1 Restriction Enzyme Digests CUT H. Smith restriction enzyme
like a scissor that cuts the restriction sites
39
Restriction Mapping
SEPARATE
40
1.5.2 Gel Electrophoresis
Fig. 1.11
41
1.5.3 Blotting and Hybridization SEARCH steps:
blotting( 漬墨法 ): gel separation ( 先分離 ) transfering ( 再固定 )
hybridization( 雜交 ): probe: chemically synthesized radioactivity, fluorescent dyes, enzyme, antibody
washing
42
43
Southern blotting (DNA), named after Edwin Southern
Northern blotting (RNA) Western blotting (proteins)
DNA chip, microarray
44
1.5.4 Cloning ( 選殖 )
PURIFY & ENLARGE DNA fragment
inserted into vectors (phage, plasmid) replicate purify
library genetic library (for DNA) cDNA library (for RNA)
45
1.5.5 Polymerase Chain Reaction AMPLIFY K. Mullis, 1985 Required
DNA polymerase: 5’ extends to 3’ primer
Repeat 20 times 1 M copies in a couple of hours
46
47
48
1.5.6 DNA Sequencing
Determine the order of nucleotides in a DNA fragment
Maxam-Gilbert method, 1970 Sanger’s Chain-termination method
49
50
1.6 Genomic Information Content (Before sequencing is available…) C-Value Paradox
The amount of DNA in every cell of a given organism is the same.
C-Value paradox: complexity & genome size are not correlated.
51
52
Reassociation Kinetics R. Britten et al, 1960s cot equation: c0t1/2 measures the weight (or amo
unt) non-repeated sequences. t1/2: time for renaturing (i.e. c/c0=0.5)
53
參考資料及圖片出處
1. Biochemistry, by J. M. Berg, J. L. Tymoczko, and L. Stryer, Fith Edition, 2001.
2. DNA的14堂課, by Karl Drlica, 1996, 周業仁翻譯 , 天下文化 , 2002 翻譯 .
3. 多數圖片來自課本Fundamental Concepts of BioinformaticsDan E. Krane and Michael L. Raymer, Benjamin/Cummings, 2003.
54
語音出處
http://www.merriam-webster.com/