Discover the UniProt Blast tool

Murcia, February, 2011Protein Sequence Databases

Customize the BLAST results

Look for the local alignment

Highlight important positions within the alignment according to the sequence annotation

The metal binding sites are conserved

HBB_HUMAN

Discover the UniProt Align tool

1- Select sequences of interest… (from Blast results for example)

2 - Align…

Add the ‘query’ sequence(HBB_HUMAN)

Conservation of the metal-binding site….

Highlight important positions within the alignment according to the sequence annotation

Compare the Blast

@ UniProt and

@ NCBI

Protein Sequence Databases

The ‘alternative’ sequence(s)

not ‘directly available’ for a lot of tools, including protein identification tools, Blast, depending on the server !….

Murcia, February, 2011

Blast P04150 against Swiss-Prot / homo sapiens @ UniProt

Isoform sequences

Murcia, February, 2011Protein Sequence Databases

Protein Sequence Databases

Blast P04150 against Swiss-Prot / homo sapiens @ NCBI

The isoform sequences (from Swiss-Prot) are not present in the NCBI protein database !The .x number (P06401.4) correspond to the version number of the sequence…not to an alternatively spliced sequence !

Murcia, February, 2011