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血清驯化在PRRS防控中的应用Application of serum acclimation/therapy
in controlling and preventing PRRS 回顾和分析
Review and analysis广西农垦永新：伍少钦 Shaoqin Wu
Email: [email protected]
广西农垦永新畜牧集团良圻原种猪场
蓝耳病控制与净化 Control and Eliminate PRRS
Biosecurity methods 生物安全措施（空气过滤）
All in all out 全进全出
Gilt acclimation( serum/modified live vaccine/field isolated live vaccine) 后备猪驯化(血清/活疫苗/田间分离活病毒)Herd Closure+ serum therapy(inoculation) 封群+驯化
Depopulation(Total or Partial esp. nursey ) 清群 (全部/部分)P1 site /P2 and P2+ site 分胎次饲养
提纲 Outline
血清驯化方法
the methods of serum acclimation/therapy全群血清驯化（血清治疗）与母猪流产
serum therapy and sow abortions 后备猪的血清驯化
serum acclimation in gilt replacement成功净化场的案例分析
one successful case of eliminating the PRRSV小结 summary
血清驯化方法Methods of Serum Acclimation/Therapy
血清制备 prepare the serum for acclimating
血清驯化过程 the process of serum acclimation
驯化后指标跟踪 Evaluate the acclimation
血清制备 Prepare the serum
寻找PRRS疑似猪，通过群体选择和个体选择确定合适的猪只，采血并标识，用ORF5引物，确定相应猪只，并送测序公司测序，比对相互间序列差异。Bleed the PRRS-like clinic pigs, get PRRS infection pigs using RT-PCR(ORF-5), get the sequence of the field virus and compare with the old one.通过荧光定量 PCR确定病毒载量，确定驯化用的血清量。
Using real-time PCR, make sure the dose of PRRS virus
血清驯化过程 Process of Serum Acclimation
血清驯化前2天对种猪群进行饲料保健，采用肌肉注射或喷鼻等方式对猪群进行驯化，驯化过程中个别不吃料的猪只，要进行相应的个体治疗。Add some antibiotic before PRRS serum acclimation by I.M. or a nasal spray. Treat some anorexia pigs with antibiotic.
驯化后跟踪Tracing some index after acclimating
驯化后第2天，随机采血30头猪PCR检测（5头/管）监测PRRSV，评估驯化是否成功。Bleeding after acclimating 2 days, random 30 pigs, detect PRRSV by RT-PCR(5 pigs/pose), evaluate the results母猪全群驯化，驯化后跟踪抗原/抗体和生产指标变化，固定30头母猪，每个月监测抗体，跟踪一年PRRS抗体的变化。If acclimating the whole farm, tracing the change of Ag/Ab and production performance, fixed 30 sows, monitor the PRRSV-Ab monthly, lasting more than 1 year.
血清驯化汇总表 Serum TherapyTime次数
事件时间 Date of happening 天数 days
SI*1 date SI2 date AT# start AT peak AT end start peak duration
1 7/14/20077/16/20077/16/20077/20/20077/27/2007 2 6 13
2 6/30/2013 7/2/2013 6/30/2013 7/6/2013 7/12/2013 0 6 12
3 2/26/20112/28/20112/26/2011 3/4/2011 3/11/2011 0 6 13
4 8/7/2007 \ 8/7/2007 8/14/20078/18/2007 0 7 11
5 3/8/2011 3/10/20113/12/20113/17/20113/21/2011 4 9 13
6 9/12/2007 \ 9/12/20079/17/20079/23/2007 0 5 11
7 9/14/2007 \ 9/15/20079/20/20079/23/2007 1 6 9
8 1/18/20141/20/20141/19/20141/22/20141/24/2014 1 4 6
9 4/9/2014 4/11/20144/12/20144/15/20144/17/2014 3 6 8
10 5/3/2015 5/5/2015 5/5/2015 5/9/2015 5/13/2015 2 6 10
11 3/26/20163/30/20163/26/2016 4/1/2016 4/2/2016 0 6 7
12 3/30/2016 4/2/2016 3/30/2016 4/6/2016 4/10/2016 0 7 11
*SI=serum inoculation#AT=abortion Average/
平均1.2 6.7 11.3
2011 ZXZ sow farm
Ab rate: 0.95%
ZXZ繁殖指标 Reproduction Performance
2011/3/8和3/10血清驯化，期间流产数无明显增加。活仔由2011年9.96头上升到10.58头。配种分娩率同86.05%上升到89.28%；由上数据分析血清驯化，PRRS的稳定期≥3年。 Herds inoculated with serum at Mar.8th and Mar. 10th . Born alive from 9.96 to 10.58, FR increased from 86.05% to 89.28%; in this farm PRRS stable status ≥3 years
Time/ year Sow bred Conception Rate Farroing Rate Born alive Total born Born alive
percent2009 2088 95.21% 80.97% 9.94 11.1 90%
2010 2401 94.46% 82.61% 10.26 11.52 89%
2011 4463 96.24% 86.05% 9.96 11.38 88%
2012 5136 97.92% 87.05% 10.38 11.69 89%
2013 5286 98.35% 89.12% 10.7 12.05 89%
2014 4325 98.29% 89.28% 10.58 12.03 88%
2015 5078 98.64% 83.61% 10.4 12.32 84%
2016 2305 98.99% 89.34% 10.9 12.46 87%
May 2015 PRRS unstable
ZXZ PRRS-ORF5 sequence
2015年11月测序的ORF 5序列，与11年的相似度只有96.5%。The sequence PRRS ORF-5 of Nov. 2015 only 96.5% similarity compare to 2011’s.
ZXZ PRRS-ORF5 sequence
采用血清驯化的猪场，猪场内部的PRRS-ORF5的序列整体比较接近。The sequence of PRRS ORF-5 is similar when using serum acclimation.
2011 HP sow farm
Ab rate: 2.87%
繁殖指标 Reproduction Performance
2011年1月17头母猪流产，2月9头流产，决定全群驯化，2月26/28号2次全群血清驯化，期间15头母猪流产，3月配种分娩率略有下降（由79.89%降至75.50%），2013年分娩率为86.83%；活仔由9.24升至10.17。Herds inoculated with serum at Feb.26th and Feb.28th. Born alive from 9.24 to 10.17, FR increased from 81.70% to 86.83%; in this farm PRRS stable status ≥3 years.
Time/ year Sow bred Conception Rate Farroing Rate Born alive Total born Born alive
percent2009 1872 94.36% 73.85% 9.14 10.27 89%
2010 2123 95.29% 77.52% 8.56 10.19 84%
2011 1981 95.52% 81.70% 9.24 10.84 85%
2012 1823 97.16% 90.32% 10.26 11.42 90%
2013 2619 97.71% 86.83% 10.17 11.27 90%
YZ sow farm
5.56%
繁殖指标 Reproduction Performance
YZ猪场2013年6月30/7-2两次血清驯化，分娩率由13年的85.04升到15年的90.49%，胎产总仔由9.70上升至10.09。2016年由于PRRS不稳定，又有所下降。
Time/ year Sow bred Conception Rate Farroing Rate Born alive Total born Born alive
percent2009 6284 89.51% 83.74% 9.63 10.95 88%
2010 5818 90.46% 84.93% 8.93 10.44 86%
2011 6070 92.29% 86.39% 9.60 10.91 88%
2012 5853 90.98% 83.26% 9.49 10.92 87%
2013 6081 92.17% 85.04% 9.70 11.40 85%
2014 5371 94.00% 87.38% 10.05 11.96 84%
2015 5308 95.86% 90.49% 10.09 11.81 85%
2016 2882 94.55% 81.68% 10.29 12.00 86%
后备猪血清驯化 Serum Acclimation in Gilts
实施PRRS阳性血清驯化后备猪，可提高母猪群配种分娩率、总仔/活仔。2009年对部分场实施PRRS血清驯化，2010年开始，所有猪场实施，血清驯化主要针对35-65公斤选留后备种猪。从EZ猪场生产数据表明（见下表），实施血清驯化是有效的。
Utilize serum acclimation for PRRS, can improve sow farrowing rate, total born and born alive. Beginning in one sow farm in 2009, all farms of YX started serum acclimation for gilts in 2010. the serum acclimation is very effect as to the data of EZ sow farm.
EZ生产指标 Reproduction Performance
Time/ year Sow bred Conception Rate Farroing Rate Born alive Total born Born alive
percent2008 3496 86.76% 76.63% 9.94 11.27 88%
2009 3246 95.01% 80.38% 9.64 11.12 87%
2010 3281 96.86% 83.36% 9.88 11 90%
2011 3181 98.33% 89.37% 10.07 11.08 91%
2012 3890 98.23% 86.07% 9.62 10.76 89%
2013 5502 98.73% 88.84% 10.03 11.35 88%
2014 4957 98.99% 79.02% 10.45 11.88 88%
2015 4728 98.94% 88.40% 10.24 11.92 86%
2016 2676 99.35% 91.16% 10.48 12.3 85%
成功净化PRRS的案例Successful case of eliminating PRRSV in a sow farm
血清驯化过程 Serum Inoculation
时间 Time 说明 comments2014-1-18 全群母猪+后备，1101头基础母 all sows + gilts
2014-1-20驯化期间(2周）流产10头，死淘13头abortion 10, cull 13 during serum therapy( in 2 weeks)
血清驯化+封群是控制或净化PRRS有效的方法。2013年冬天猪场变得PRRS不稳定，2014年1月决定血清驯化（包括6个月以上后备猪）和封群。Serum inoculation (therapy) with herd closure is a good method for control or eliminating PRRS in sow farm. The farm had 1200 sows in 2014. The status of PRRS became unstable in 2013 winter. According with clinical signs, we decided to inoculate PRRSV serum to sows and gilts, and then closed the herd.
Sow abortion of acclimation 驯化前后流产情况
母猪跟踪检测 ELISA Ab( IDEXX)—Sow
公猪跟踪检测 ELISA Ab( IDEXX)—Boar
公猪跟踪检测 ELISA Ab( IDEXX)—Boar
每年常规检测 ELISA PRRS-Ab (IDEXX)2014
PRRS boar sow gilt piglet nursey finishsample 266 471 75 52 264 530
>0.4 60% 52% 45% 19% 14% 13%>2.5 3% 2% 0% 2% 0% 0%>1.5 14% 10% 5% 4% 2% 8%Avg 0.71 0.62 0.48 0.30 0.19 0.23 CV 101 100 106 163 196 254 Std 0.71 0.62 0.51 0.50 0.38 0.57
2015PRRS boar sow gilt piglet nursey finish
sample 247 408 221 725 441 221 >0.4 2% 24% 1% 5% 0% 0%>2.5 0% 0% 0% 0% 0% 0%>1.5 0% 1% 0% 0% 0% 0%Avg 0.06 0.27 0.04 0.10 0.04 0.01 CV 195 132 380 198 197 230 Std 0.11 0.35 0.14 0.21 0.07 0.02
2016 PRRS boar sow gilt piglet nursey finish
sample 75 231 283 538 391 1,002 >0.4 3% 13% 1% 1% 0% 0%>2.5 0% 0% 0% 0% 0% 0%>1.5 0% 0% 0% 0% 0% 0%Avg 0.05 0.16 0.05 0.06 0.04 0.02 CV 158 152 200 150 150 298 Std 0.09 0.24 0.10 0.09 0.06 0.06
母猪场PRRS抗体检测结果(2014-2017)项目 年份 公猪 母猪 后备猪 仔猪 保育 育肥
样本
2014 266 471 75 52 264 5302015 247 408 221 725 441 2212016 116 273 572 1066 1007 1128
20170804 48 153 789 709 657 1408
s/p>0.4 阳性
2014 60% 52% 45% 19% 14% 13%2015 2% 24% 1% 5% 0% 0%2016 2% 12% 1% 0% 0% 0%
20170804 0% 5% 1% 0% 0% 2%
s/p 平均值
2014 0.71 0.62 0.48 0.3 0.19 0.232015 0.06 0.27 0.04 0.1 0.04 0.012016 0.03 0.14 0.04 0.04 0.02 0.02
20170804 0.01 0.07 0.03 0.02 0.01 0.04
生产数据 Reproduction data
Time/ year Sow bred Conception Rate Farroing Rate Born alive Total born Born alive
percent2013 1057 98.68% 90.82% 9.95 11.43 87%
2014 3024 98.97% 84.26% 9.83 11.17 88%
2015 2782 98.49% 89.14% 9.91 11.58 86%
2016 2615 93.75% 90.07% 9.72 11.6 84%
2017 1472 92.40% 89.66% 10.21 11.8 87%
血清驯化小结 Serum therapy summary Farm code
Date of happening daysSI*1 date SI*2 date AT# start AT# peak AT# end start peak duration
F1 7/14/2007 7/16/2007 7/16/2007 7/20/2007 7/27/2007 2 6 13F1 6/30/2013 7/2/2013 6/30/2013 7/6/2013 7/12/2013 0 6 12F5 2/26/2011 2/28/2011 2/26/2011 3/4/2011 3/11/2011 0 6 13F4 8/7/2007 \ 8/7/2007 8/14/2007 8/18/2007 0 7 11F4 3/8/2011 3/10/2011 3/12/2011 3/17/2011 3/21/2011 4 9 13F3 9/12/2007 \ 9/12/2007 9/17/2007 9/23/2007 0 5 11F2 9/14/2007 \ 9/15/2007 9/20/2007 9/23/2007 1 6 9F8 1/18/2014 1/20/2014 1/19/2014 1/22/2014 1/24/2014 1 4 6F6 4/9/2014 4/11/2014 4/12/2014 4/15/2014 4/17/2014 3 6 8F4 5/3/2015 5/5/2015 5/5/2015 5/9/2015 5/13/2015 2 6 10F6 3/26/2016 3/30/2016 3/26/2016 4/1/2016 4/2/2016 0 6 7F7 3/30/2016 4/2/2016 3/30/2016 4/6/2016 4/10/2016 0 7 11#AT=abortion *SI=serum inoculation average 1.0 6.2 10.4
结论 Summary
后备猪的血清驯化值得去做，尤其不免疫蓝耳苗的猪场；Serum acclimation –useful tool for gilts, esp. for Non-vaccine farm整个母猪群血清驯化，可尝试尤其是疫苗无效时，但有风险；
Serum inoculation (therapy) also is a tool, esp. no effect with vaccine, but it has risk.血清驯化+封群+一次引够后备，加强生物安全，可做到PRRS净化。
Serum therapy + herd closure + 6 months gilt(acclimated the same time) + strengthen farm biosecurity >>> eliminate PRRS
推广价值 Extension value
2007年良圻原种猪场开始在母猪群中应用血清驯化控制蓝耳病，2009年开始在后备猪中用血清驯化稳定蓝耳病。在国内养猪企业中，良圻原种猪场是最早应用血清驯化控制PRRS的猪场之一。通过11年的血清驯化工作，加上兽医技术中心的监测（抗体检测，抗原检测、测序和序列分析、荣光定量PCR等），良圻原种猪在蓝耳病控制和净化方面积累了大量的第一手资料。
Liangqi Farm applied serum therapy to control PRRS in sows during 2007, applied with serum acclimation in gilt during 2009. LiangqiFarm is one of the first farms applied serum acclimation for control PRRS. We has lots of experiences in controlling and eliminating PRRS
推广价值 Extension value
血清驯化的时机非常关键，血清驯化不一定能保证100%成功，其中有一些不可预料因素，是否决定全群血清驯化，有时是一个艰难的抉择；但是只要充分了解本场的健康状况，同时加上周密计划，血清驯化的成功率还是很高的。良圻原种猪场在PRRS控制与净化方面的多年实践，为国内蓝耳病的控制与净化提供了宝贵的经验，值得国内外的养猪同行参考与借鉴。The timing for serum acclimation is very critical, we can’t guarantee all successful when apply serum acclimation/therapy, many uncertain factors . Sometime it is a dilemma to do or not do serum acclimation. But if we know much more the farm and prepare in advance, we can get good results from it . What we did in past 11yeats provide some important references for swine producers.
致谢 Acknowledgment
吴志君总经理Mr. Zhijun Wu闫之春博士 Dr. Jason Yan朱汉守教授 Dr. HanSoo Joo莫里森教授 Dr. Bob Morrision谢体三博士 Dr. Tishan Xie曾容愚博士 Dr. Rongyu Zeng马 玲 博士 Dr. Ling Ma良圻兽医技术中心 Vet diagnose Lab of Yongxin Husbandry company
农垦永新良圻全体人员 Employees of State Farms Yongxin Husbandry company
Critiques welcome!
