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Takeda et al., Supplemental Figure 1. (A). (B). A / (A+G). Sequence Height. Sequence Height. A / (A+G). pRL-TK-4x A /4x G mix A/(A+G). pRL-TK-3’ A /3’ G mix A/(A+G). (C). (D). GeneScan Area. A / (A+G). Sequence Height. A / (A+G). gDNA mix AA/(AA+GG). gDNA mix AA/(AA+GG). - PowerPoint PPT Presentation
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gDNA mix AA/(AA+GG)
(A)
Takeda et al., Supplemental Figure 1
(C)S
equ
en
ce H
eig
ht
A /
(A+
G)
Seq
ue
nce
He
igh
t A
/ (A
+G
)S
equ
en
ce H
eig
ht
A /
(A+
G)
(B)
(D)
gDNA mix AA/(AA+GG)
pRL-TK-4xA/4xG mixA/(A+G)
pRL-TK-3’A/3’G mixA/(A+G)
Gen
eS
can
Are
a A
/ (A
+G
)
HL HL HL HL HL HLSonication
(A)
Takeda et al., Supplemental Figure 2
Input Sup IP
No Fixation
miRNA 1 1M 1 1M 1 1M 1 1M 1 1M 1 1M 1 1M 1 1M 1M 1 1M 1 1M 1 1M
HL HL HHL HL HL
Input Sup IP
With Fixation
*hAGOs
h
l
Input
IP
HL HL HL HLSonication
miRNA
RT + - + - + - + - + - + - + - + -
(B)
1 1M 1 1M 1 1M 1 1M
No Fixation With Fixation
Log 2
[ (
A/G
IP)
/ (A
/Gin
p)
]
Takeda et al., Supplemental Figure 3
Log 2
[ (
A/G
) /
(A/G
gD
NA)
]Input IP
