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EuropeAll about PCR and Real Time PCR

LongGene Europe

2 www.longene.com.tr

10’’ TFT LCD Color Touch Screen Real –time monitoring of PCR RunningLight Sources (each channel): LEDSensor (each channel): New generation SSLP CCD Technology: T-OpticalTM

Detection Sensitivity: ≥ 1 copyDynamic Range: 1-1010 (%99.7 reliability)Block Type : Gradient PeltierCapacity: 96x0.1 ml (singular /8 strips and 96 well)Sample Volume: 10-50 ulBlock Temperature Range: 0-105 ºCTemperature Sensitivity: ≤± 0.2 ºC (at 90 ºC)Temperature Accuracy : ≤± 0.2 ºC (at 90 ºC)Active Temperature Tracking Resolution: 0.1 ºCHeating Rate: 6 ºC /second (max)Cooling Rate: 5 ºC /second (max)Gradient Range: 30-100 ºCGradient Capacity: 1-30 ºC (12 column)Heat Lid Temperature Range: 30-112 ºCFluorescence Excitation Range: 300-800 nmFluorescence Detection Range: 500-800 nmConnection: USB, LANChannel-1: FAM, SYBR GREEN (1/2)Channel-2: VIC, HEX, JOE, CY3, NEDChannel-3: ROX, TEXAS-REDChannel-4: CY5Channel-5: CY5.5Channel-6: RESERVEISO, IVD-CE, PCR PERFORMANCE WARRANTY, POC

The device is used for Real Time PCR (Polymera-se Chain Reaction) applications for DNA and RNA (cDNA) amplifications.

Genetic Disease Detection Virus or Pathogen detection Drug Resistance Analysis DNA Methylation Study SNP (Single Nucleotide Polymorphism) Gene Expression Studies Multiplex Analysis IVD Applications (Covid19, Variant Genotyping, Hepatitis, Respiratory infections etc.)

New generation SSLP CCDs and T-OpticalTM top detection tech-nology reduces background noise. It has 6 °C heating and 5 °C cooling performance with new generation peltier elements with 1 million cycle life. Protocols can be started both on the device and from USB li-censed OptimalTM computer control and analysis software.Analyzes can be done on different computers.

Open SystemSuitable for using with different brands of consumables and kits. The device can also be used as a conventional PCR device. (Thermal Cyc-ler). Sybr green dyes and 2/4/6 Multiplex reading into a single tube with taqman probes.Has an internal 15.000 protocol capacity.

Absolute Quantification, Melting Curve, Relative Quantitation (ΔCt, ΔΔCt), SNP Genotyping, Pathogen Positive/Negative, etc..

Compatible with IVD products of all brands.

Q2000A Model: 2 Channels (supports 7 universal dyes) Q2000B Model: 4 Channels (supports 10 universal dyes) Q2000C Model: 6 Channels (supports 11 universal dyes)

Technical Basic Specifications

Purpose

Scope of Application

New Generation fast qRT-PCR Device

LongGene | Q2000 Series

REAL TIME PCR INSTRUMENTS

www.genplaza.com 3

LongGene Europe

Q1000 Model: 2 Channels (supports 7 universal dyes) Q1000+ (Plus): 4 Channels (supports 10 universal dyes)

New generation SSLP CCDs and T-OpticalTM top detection tech-nology reduces background noise.It has 7 ºC heating and 5 ºC cooling performance with new generation peltier elements with 1 million cycle life. Protocols can be started both on the device and from USB licensed Opti-malTM computer control and analysis software. Analyzes can be done on different computers.

Open SystemSuitable for using with different brands of consumables and kits. The device can also be used as a conventional PCR device. (Thermal Cyc-ler). Sybr green dyes and 2/4 Multiplex reading into a single tube with taqman probes. Has an internal 15.000 protocol capacity.

Absolute Quantification, Melting Curve, Relative Quantitation (ΔCt, ΔΔCt) ,SNP Genotyping, Pathogen Positive/Negative, etc..


New Generation fast qRT-PCR Device

7’’ Color Touch Screen Real –time monitoring of PCR RunningLight Sources (each channel): LEDSensor (each channel): New generation SSLP CCDTechnology: T-OpticalTM

Detection Sensitivity: ≥ 1 copyDynamic Range: 1-1010 (%99.7 reliability)Block Type : Gradient PeltierCapacity: 48x0.1 ml (singular /8 strips and 48 well)Sample Volume: 10-50 ulBlock Temperature Range: 0-105 °CTemperature Sensitivity: ≤± 0.2 °C (at 90 °C)Temperature Accuracy : ≤± 0.2 °C (at 90 °C)Active Temperature Tracking Resolution: 0.1 °CHeating Rate: 7 °C /secondCooling Rate: 5 °C /secondGradient Range: 30-100 °CGradient Capacity: 1-24 °C (8 column)Heat Lid Temperature Range: 30-112 °CFluorescence Excitation Range: 300-800 nmFluorescence Detection Range: 500-800 nmConnection: USB, LANChannel-1: FAM, SYBR GREEN (1/2)Channel-2: VIC, HEX, JOE, CY3, NEDChannel-3: ROX, TEXAS-REDChannel-4: CY5

ISO, IVD-CE, PCR PERFORMANCE WARRANTY, POC




Purpose




LongGene Europe


LONGGENE │OPTIMAL ANALYSIS SOFTWARE

Auto/Manual Normalization

Auto/Manual Threshold

Auto/Manual Baseline

Auto Gain Function(Calculated automatically)

Absolute QuantificationSoftware automatically draws the ‘standard line’ according to the standards you know the amount (number of copies) and gives the number of copies according to the Ct (thres-hold) values of your samples.Plate view and curves on one screen.Other important features of the software;• Quality Control (QC) function• Creating ready to use protocols• Functional reporting• Compatible with Windows XP/7/10

(32Bit/64Bit)

SNP (Single Nucleotide Polymorphism)Genotyping analysis

High resolution graphs and fluorescence for Melting Curve

www.longene.com.tr 5

LongGene EuropeLONGGENE │OPTIMAL ANALYSIS SOFTWARE

PLUS/MINUS Fast pathogen detection and analysis

Relative QuantificationIt creates a statistical report of different gene regions according to the obtained Ct values; Automatically calculates and reports ΔCt Mean, ΔCt SE, ΔΔCt, RQ, RQ Min and RQ Max.The reporting features of the software;• Txt, doc, xls and pdf export• Automatic result according to definitions• Post-study re-analysis• Free lifetime update• Compatible with Windows XP/7/10

(32Bit/64Bit)

Taqman probes and Syber dyes Multiplex analysis in a single tube

Auto Efficiency Calculation

Plate or List View

Functional Report Options

Copy/Past Your Sample Names(Horizontal or Vertical) from an Excel file, easily

LongGene Europe






Purpose


7’’ Color Touch Screen Real –time monitoring of PCR RunningLight Sources (each channel): LEDSensor : New generation SSLP CCD Detection Sensitivity: ≥1 copyDynamic Range: 10-1010 (%99.7 reliability)Block Type : Peltier Capacity: 16x0.1 ml (singular /8 Strips)Sample Volume: 10-100 ulBlock Temperature Range: 4-100 °CTemperature Sensitivity: ≤± 0.25 °C (at 90 °C)Temperature Accuracy : ≤± 0.25 °C (at 90 °C)Active Temperature Tracking Resolution: 0.1 °CHeating Rate: 5 °C /second (max)Cooling Rate: 4 °C /second (max)Heat Lid Temperature Range: 30-112 °CFluorescence Excitation Range: 470-500 nmFluorescence Detection Range: 520-700 nmConnection: USB, LANData Transfer: EXCEL, TXTChannel-1: FAM, SYBR GREEN (1/2)Channel-2: VIC, HEX, JOE, CY3, NEDChannel-3: ROX, TEXAS-REDChannel-4: CY5

ISO, IVD-CE, PCR PERFORMANCE WARRANTY, POC


New generation SSLP CCDs and T-OpticalTM top detection tech-nology reduces background noise.It has 5 ºC heating and 4 ºC cooling performance with new generation peltier elements with 1 million cycle life. Protocols can be started both on the device and from USB licensed Opti-malTM computer control and analysis software. Analyzes can be done with/without a computer.

Mini qRT-PCR Device

LongGene Q160 Model: 2 Channels (supports 5 universal dyes) LongGene Q160C Model: 4 Channels (supports 10 universal dyes)

Open SystemSuitable for using with different brands of consumables and kits. The device can also be used as a conventional PCR device. (Thermal Cyc-ler). Sybr green dyes and 2/4 Multiplex reading into a single tube with taqman probes. Has an internal 15.000 protocol capacity.

Absolute Quantification, Melting Curve, Relative Quantitation (ΔCt, ΔΔCt) ,SNP Genotyping, Pathogen Positive/Negative, etc..


96x0,2ml + 77x0,5ml Gradient Peltier Block

PCR Performans Garanti Belgesi

Model: A600

Yeni Nesil Gradient PCR Cihazõ

Yeni nesil peltier elementler ise saniyede 6 ¡CÕlõk õsõtma ve soğutma hõzõna sahiptir. Gradient bloğu hem 0,2mlÕlik hem de 0,5mlÕlik tŸplerle kullanõma uygundur.

96x0,2ml + 77x0,5ml Kapasiteli Gradient Blok

Tak kullan Gradient bloğu sayesinde aynõ anda 12 farklõ bağlanma sõcaklõğõ ile çalõşõr, .

Primer Optimizasyonu için ideal model; 6 Adet 16x0,2ml kapasiteli blok

8Õli strip veya 96 PCR Plakalarõna uygun (Toplam kapasite: 96x0,2ml)

Her biri gradient olan 6 ayrõ bšlge Programlama Aralõğõ : 4-100 ¡C Isõtma hõzõ saniyede : 5,5 ¡C

Soğutma hõzõ saniyede : 4,5 ¡C Her bšlgede farklõ bağlanma sõcaklõğõ..

Sõcaklõk Doğruluğu: ≤ ± 0,15 ¡C Model: A600 Sõcaklõk Eş Dağõlõmõ ≤ ± 0,20 ¡C

Kullanõm Amacõ

Cihaz, PCR (Polimeraz Zincir Reaksiyonu) y š n t e m i i l e D N A v e R N A Õ n õ n AmpliÞkasyonu (çoğaltõlmasõ) için üretilmiştir.

Uygulama Alanlarõ

Genetik Hastalõk Tespiti Virüs veya Patojen tespiti İlaç Direnç Analizleri DNA Metilasyon ‚alõşmasõ SNP (Tek Nokta Mutasyonu) Gen Ekspresyon ‚alõşmalarõ Multiplex Analiz vb..

Temel Teknik …zellikler

Kontrol Ekranõ // 7ÕÕ Dokunmatik GraÞk Blok Kapasitesi // 96x0,2ml + 77x0,5ml Sõcaklõk Aralõğ õ // 0 - 100 ¡C Isõtma/Soğ utma Hõzõ // 6 ¡C/sn Sõcaklõk Doğruluğu // ≤ ± 0,2 ¡C Ekran ..zŸnŸrlŸğü // 0,1 ¡C Gradient Aralõğ õ // 30 - 99,9 ¡C Gradient Kapasitesi // max 30 ¡C Gradient Programlama // 12 SŸtun Isõtmalõ Kapak // 30 - 112 ¡C Program Kapasitesi // 15.000 (Dahili) Auto-Restart …zelliği // VAR Şifre Korumalõ kullanõcõ hesaplarõ Boyutlar : 36,2 x 25,6 x 25,5 cm (G,D,Y) Ağõrlõk : 7,3 kg

Bağlantõ ArayŸzleri LAN (PC), 2 Adet USB 2.0

Dahili Hesaplayõcõlar Gradient Hesaplayõcõ // VAR Tm Hesaplayõcõsõ // VAR PCR Performans Belgesi, ISO 9001, CE

THERMAL CYCLER (PCR) CİHAZI

LongGene | A300

It has 6 °C heating performance with new generation peltier el-ements with 1 million cycle life. Gradient block is suitable for use with both 0.2 ml and 0.5 ml tubes. Device systems has the functions of increasing/decreasing the time per cycle (1-20s) and increasing/decreasing the temperature per cycle (1-10 °C).

Graphically protocol edit and display the whole running.

Easily interchange the blocks without tools

Optional blocks for choice Interchangable block and adaptor options are avaliable (384 Well Block, In-Situ Adaptor)

15,000 protocols storable on board and unlimited stonge by USB disk.

A600 Super Gradient Thermal Cycler PCR(Ideal for Research, especially for primer optimizations)

Block Tube Capacity: 96x0.2 ml (single/8 strip/96 well plate)

Zone Capacity: 6 saperate zones, 6 different annealing temperatures

Gradient Capability: 6 individual gradient on each zones, 4 column

Gradient Capacity: 6 separate regions, each of them are gradient internally

Gradient Range: 1–25 °C

Temperature Range: 0-105 °C

Heating Capacity: 5.5 °C/s (max)

Cooling Capacity: 4.5 °C/s (max)

Temperature Accuracy: ≤± 0.15 °C (at 95 °C)

Temperature Uniformity: ≤± 0.15 °C (at 95 °C)

Display Resolution: 0.1 °C

New Generation Gradient PCR Device


LongGene Europe

The device is used for PCR (Polymerase Chain Rea-ction) applications for DNA and RNA (cDNA) amp-lifications.


Purpose


Control screen: 7’’ Graphic Touch Screen Block Capacity: 96x0,2 ml + 77x 0,5 ml (opt)

Temperature Range: 0-105 °C

Heating Rate: 6 °C /second (max)

Cooling Rate: 5 °C /second (max)

Temperature Accuracy : ≤± 0.1 °C

Temperature Uniformity: ≤± 0.2 °C

Screen Resolution: 0.1 °C

Gradient Range: 30-99.9 ºC

Gradient Capacity: 1-30 ºC (12 column)

Heat Lid Temperature Range: 30-112 °C

Programe Capacity: 15.000 (internal)

Auto-Restart Property: Available

Password Protected Accounts: Available

Dimensions : 36.2 X 25.6 X 25.5 cm

Weight: 7.3 kg

Connection: LAN (PC), 2x USB (2.0)

Built-in calculators: Gradient calculator, Tm calculator

CEISO 9001 PCR PERFORMANCE WARRANTY POC


LongGene | A300

THERMAL CYCLER (PCR) INSTRUMENTS

LongGene Europe

8 www.genplaza.com

LongGene | T20/T30

The device is used for PCR (Polymerase Chain Rea-ction) applications for DNA and RNA (cDNA) amp-lifications.

Genetic Disease Detection Virus or Pathogen detection Drug Resistance Analysis DNA Methylation Study SNP (Single Nucleotide Polymorphism) Gene Expression Studies Multiplex Analysis IVD Applications (Covid19, Variant Genotyping, Hepatitis, Respira-tory infections etc.)

Purpose


Control screen: 10’’ Graphic Touch Screen Block Capacity: 3x32 (0.2 ml) / 2x48 (0.2 ml)

Temperature Range: 4-99.9 °C

Heating Rate: 7.5 °C /second (max)

Cooling Rate: 6 °C /second (max)

Temperature Accuracy : ≤± 0.2 °C

Temperature Uniformity: ≤± 0.2 °C

Screen Resolution: 0.1°C

Gradient Range: 30-99.9 °C

Gradient Capacity: 1-25 °C (8 column)

Heat Lid Temperature Range: 30-112 °C

Programe Capacity: 15.000 (internal)

Auto-Restart Property: Yes

Password Protected Accounts: Yes

Dimensions : 27.0 x 37.5 x 27.7

Weight: 13 kg

Connection: LAN(PC), USB (2.0)

Built-in calculators: Gradient calculator, Tm calculator

CE

ISO

PCR PERFORMANCE WARRANTY

POC

Technical Basic SpecificationsT30 3x32 (0.2 ml) Gradient Peltier Block

T20 2x48 (0.2 ml) Gradient Peltier Block

It has a 7 °C heating and 6 °C cooling speed per second with the new generation peltier elements. Ensure the long life with 1.000.000 cycles.

New Generation Gradient PCR Device

2 or 3 Individual sample blocks allow 2 or 3 different protocols running at the same time.Easily interchange the blocks without tools.New lever-style heat lid could lock up the lid pressure automatically, ensure during running of protocol.

THERMAL CYCLER (PCR) INSTRUMENTS


LongGene Europe

It is produced to minimize labor and user errors by allowing au-tomatic preparation of reagents and nucleic acids in PCR or Real Time PCR studies. The device prepares the mix for PCR or Real Time PCR Devices using 384 and 96 plates.

PCR Mixing/Sample Preparation

Mix/Sample Preparation for Real Time PCR

Preparation for the sequence

HLA applications

DNA normalization

Chip SNP studies

Elisa Plate Preparation

Covid-19 RT-PCR plate setup etc.

Arise Brand EzMate Series Fully Automatic Pipetting Systems

FeaturesExchangeable 1, 4 and 8-channel, 50 or 200µl, Au-tomated Pipetting Modules (APMs)

Intuitive PC software: EzStarter™

CoolBlock™ for sensitive reagents/ samples

Applications: PCR/qPCR, DNA sequencing, HLA ty-ping, and SNP detection, etc.

6-plate SBS worktable capacity for middle-through put sample preparation

Easy and Convenient to UsePC Software: EzStarter™ can be mastered in one hour. No trained technician required.

Built-in PCR/qPCR setup protocols can be quickly modified and transferred via USB memory stick.

1, 4 and 8-channel, 50 ul or 200 ul, Automated Pi-petting Modules (APMs) can be exchanged without tools.

4 interchangeable standard microtiter plate/tip rack adapters and 2 interchangeable reagent vessel adapters.

Other applications: Sequencing setup, HLA typing and SNP detection, etc.

Easy to AffordThe most affordable Automatic Pipetting System available.

EzTip™ robotic tips are compatible with Beck-man’s® Biomek® 3000 system.

The CoolBlock™ adapters keep sensitive reagents/samples at 7 °C for more than 60 minutes.

Save reagent costs by reducing human errors and using more dense plates.

Easy to ServiceThe Automated Pipetting Module (APM) is simple to remove and can be mailed-in for service and ca-libration.

PC software is upgradable via the internet.

The entire system is compact and light-weighted.

Accurate and PreciseEach Automated Pipetting Module (APM) is calibra-ted using ISO-8655 standards.

Excellent results for qPCR standard curve and rep-licates.

Better Precision of pipetting results than manual pi-petting.

Arise | EzMate 401/601 (S)

FULLY AUTOMATIC PIPETTING SYSTEMS

UV SAFETY HEPA

LongGene Europe


LongGene PCR Strip Tubes & Caps

PCR Strip tube of other brands

PCR Strip Tube with Cap 96 Well RT-PCR Plate Sealing for Plate

LGT001-E LGP001-E LGS001-E

• Thin and highly uniform• Durable at low and high temperatures• High heat transfer efficiency• Maximum heating temperature: 150 °C• The lowest storage temperature: -86 °C• Medical Polypropylene• Purity is 99.9 %• Numbered wells (96 Well: A to H, 1 to 12)• Compatible with similar RT-PCR instruments

• Maximum heating temperature: 150 °C

• Polyethylene, ultra thin

• Durable and optically clear

• Good sealing performance

• Easy to peel off• Low residue• Free from DNase, RNase• Non Pyrogenic

Real Time PCR Lab Equipments: Real Time PCR

Biosafety Cabinet (Class II A2 or B2)

Digital/Electronic Pipettes (with stand)

Benchtop Cooler (to prepare the mixes at +4 °C)

Mini Spin (8-strips) or Plate Centrifuge (to use with 8-strip tubes)

Plate Centrifuge (to use with 96 well RT-PCR plates) Vortex

Disposables; Diagnostic Kit (RT-PCR test kit)

RNA Prep Solution (Virus RNA collection)

Swap (Sample collection)

0.1 ml white 8-Strip RT-PCR tubes or 96 well Plates

Pipette Tips (with rack, with filter,

DNAse/RNAse free, sterile)


LongGene Europe

RESULT INTERPRETATION AND APPLICATION SUPPORT

TURNKEY RT-PCR LAB PROJECTS

Ct

Cycle

Fluo

resc

ent

N1,N2

RP (EC)

POSİTİVE CONTROL (PC)

Ct

Cycle

N1, N2

NEGATİVE CONTROL (NC)

Ct

POSITIVE Patient

CtN1N2

N1, N2

RP (IPC)

Ct

Add reaction mix into each reaction tubes

NTC

Sample1PC

N1, N2 (FAM): 1st and 2nd regions specific to Covid-19RP (HEX): RP gene for extraction control (EC)*** Both channel must give the amplified signals.

N1, N2 (FAM): NONE (-)RP (HEX): NO (-)*** None of them give amplification in NTC.

N1/N2 (FAM) » Ct: ≤ 35 (+)RP (HEX) » 20 ≤ Ct ≤ 35 (+)Although RP (-), the result does not change.

NEGATIVE PatientN1, N2 (FAM) » Ct: No value (-)RP (HEX) » 20 ≤ Ct ≤ 35 (+)

N1,N2RP

REPEAT This PatientN1, N2 (FAM) »Ct: No value (-)RP (HEX) » Ct: No value (-)

Real Time PCR Result Interpretation Guide

RP (EC)

RP (IPC)

Sample2

Sample3 Sample

4

Sample5 Sample

6

Multiplex SARS-CoV-2 Real-time RT-PCR Kit

Fluo

resc

ent

Fluo

resc

ent

Sample Types:1) NTC2) PC3) Sample

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Son olarak; Aşağıdaki kalite belgelerini okunmayacak şekilde aşağıdaki haliyle

Aşağıdaki gibi cihaz fotoğrafını küçültüp sola yer açıp dizelim;


Tek satır yapalım, Vortex bir üst satıra çıksın; Mini Spin (8-strips) or Plate Centrifuge

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www.longgene.com.tr

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gen plaza katalog 2021 secilen-sayfalar ingilizce