kidney autopsy techniques

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    Examination of the Kidney

    Ruthashini R Selvasingam

    0902005207

    The methods and detail for the investigation of the urogenital organs depends on the gender

    of the patient. There are two main techniques in examining the kidney which are the external

    and internal examination. An adult kidney measures approximately 11 6 3cm. The kidney

    of an adult men weighs around 150g, which is slightly lesser than the women. It would be

    difficult to recognise chronic disease of an atrophic kidney. A detail attention to the

    anatomical area with histology of the dominant soft tissue would help in identifying at least

    some residual parenchyma. Fats surrounding the kidney should be cleared away beforeweighing. On the internal examination, a longitudinal, sagittal slice is done with a large-

    bladed knife, via the kidney from the convex area towards the hilum .The kidney must be

    held firmly, placing it on the flat dissecting board and anchored with a sponge using the non

    cutting hand as the blade is drawn across the kidney. Alternatively, the kidney can be held

    between the arms of large pair of forceps where the hilar surface faces down towards the

    dissection table or board with the peripheral surface facing the uppermost. The blade of the

    knife should be carefully positioned on the angle of the forceps and is moved downwards

    towards the hilum and dissecting board. Parenchyma would be easily demonstrated via this

    technique. This technique would allow assessment of the boundaries of cortical and

    medullary areas. As the slice is extended towards the pelvis, the hilar vessels can then be

    inspected. Any mass or material within the kidney and pelvis can be easily recognised. Stones

    should be removed and their size and quality must be noted. The kidney capsule should be

    held with toothed forceps where the incision has been made previously. The capsule should

    be lifted off the outer cortex and opened back to reveal the sub capsular surface. The

    appearance of the normal sub capsular surface will be smooth. The usual dissection of the

    kidney is concluded as follow where first remove the surrounding fat. Then slice through the

    convexity in a sagital direction followed by stripping of the capsule. Inspect the cortex and

    medulla subsequently. If required, blocks for histology can be taken. Special techniques can

    be used in examining kidneys containing tumours. Renal tumours can be visualized with the

    techniques described earlier unless the mass is extremely large and particularly if it invades

    perinephric tissue. The renal vein is inspected to identify vascular invasion which is a

    common feature of these tumours. It is suggested that renal vein should be assessed

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    histologically in cases of renal cell carcinoma. Examining the transplanted kidneys are

    slightly different as they are localed within the pelvis, " plumbed into" the iliac arterial and

    venous vessels. The kidney have to be removed in continuity with its vascular connections by

    dissecting it free and reflecting it medially while posterior adhesions are divided. Following

    this, the vessels are open to check sites of anastomoses if there is any intraluminal

    obstructions.