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Ejemplo de calculos utilizados en el analisis de proteinas
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Valores hallados experimentalmente
Muestra vol HCl Vm-Vb %NitrogenoBlanco 3.68 0.00 0.000 0 NAHarina 1 5.60 1.92 0.712 4.45 NAHarina 2 12.90 9.22 3.421 21.38 NAAlbuminas 20.75 17.07 0.6314 3.95 18.46Globulinas 16.45 12.77 0.47 2.95 13.81Prolaminas 7.90 4.22 0.16 0.98 4.56Glutelinas 29.65 25.97 0.96 6.00 28.08
13.88
Datos reportados en la literaturaEspecie Proteina total
Arveja 21 66 12 1 21-26%
%de proteina en la muestra
%de la especie en la proteina total
Albuminas(g/100 g de proteína)
Globulinas(g/100 g de proteína)
Glutelinas(g/100 g de proteína)
Prolaminas (g/100g de proteína)
Valores hallados experimentalmente
[HCL] muestra (mg) Muestra vol HCl %Nitrogeno0.0106 0 Harina 1 5.60 0.712 4.4520.0106 40 Harina 2 12.90 3.42 21.380.0106 40 Albuminas 40.75 1.37 8.570.0106 2006 Globulinas 16.45 0.47 2.950.0106 2006 Prolaminas 7.90 0.16 0.980.0106 2006 Glutelinas 29.65 0.96 6.000.0106 2006
%de proteina en la muestra
Tubo Concentracion Abs 540 nm1 0.1 0.0432 0.3 0.0783 0.5 0.1244 0.7 0.1645 0.9 0.2156 1.2 0.2247 1.5 0.277
Tubo F. proteica Abs 540 nm Concentracion Sln original8 albuminas 0.092 0.331 3.313 4.1299 albuminas 0.18 0.817 4.083 5.0898 Globulinas 0.05 0.137 1.371 1.7099 Globulinas 0.072 0.228 1.139 1.4208 Prolaminas 0.03 0.067 0.673 0.8399 Prolaminas 0.09 0.311 1.555 1.9388 Glutelinas 0.049 0.133 1.333 1.6619 Glutelinas 0.107 0.421 2.106 2.625
Datos reportados en la literaturaEspecie
Arveja 21
% Proteina la muestra
Albuminas(g/100 g de proteína)
19.3139086823.8007228
7.9926493186.6414011693.9233355219.0626532257.77085279112.27638138
Datos reportados en la literatura
66 12 1 21-26%
%de la especie en la proteina total
Globulinas(g/100 g de proteína)
Glutelinas(g/100 g de proteína)
Prolaminas (g/100g de proteína)
Proteina total
0 0.2 0.4 0.6 0.8 1 1.2 1.4 1.60.0
0.1
0.2
0.3f(x) = 0.166934235976789 x + 0.0367059961315281R² = 0.967839529489238
Metodo de Biuret
[Proteina]
Abso
rban
cia 5
40 n
m
Tubo Concentracion Abs 280 nm1 0.2 0.1912 0.4 0.3423 0.6 0.5064 0.8 0.6955 1.0 0.8546 1.2 0.955
Tubo F. proteica Abs 280 nm Concentracion sln original % Proteina8 albuminas 2.623 3.266 16.330 20.358 Globulinas 2.018 2.502 12.511 15.598 Prolaminas 1.056 1.288 6.439 8.028 Glutelinas 1.898 2.351 11.754 14.65
58.62
F. proteica Abs 280 nm % Proteinaalbuminas 2.623 20.35Globulinas 2.018 15.59Prolaminas 1.056 8.02Glutelinas 1.898 14.65
Tubo F. proteica Abs 280 nm Concentraciosln original % Proteina7 albuminas 1.808 2.237 22.371 27.887 Globulinas 1.53 1.886 18.861 23.517 Prolaminas 1.064 1.298 12.978 16.177 Glutelinas 1.211 1.483 14.834 18.49
86.05
0 0.2 0.4 0.6 0.8 1 1.2 1.40
0.2
0.4
0.6
0.8
1
1.2
f(x) = 0.792142857142857 x + 0.036R² = 0.994425503612054
Absorcion U.V
[Proteina] mg/ml
Abso
rban
cia 2
80 n
m
Tubo Concentracion Abs 280 nm1 0.032 0.1532 0.064 0.3073 0.12 0.3534 0.16 0.4125 0.24 0.4176 0.32 0.485
Tubo F. proteica Abs 5950 nmConcentracion sln original % Proteina7 Prolaminas 0.058 0.013 0.318 0.407 Glutelinas 0.316 0.090 2.246 2.80
muestra Abs 5950 nm % ProteinaProlaminas 0.058 0.44Glutelinas 0.316 2.83
0 0.05 0.1 0.15 0.2 0.25 0.3 0.350
0.1
0.2
0.3
0.4
0.5
0.6
f(x) = 0.13198820509467 ln(x) + 0.634101332527071R² = 0.952103312541384
Metodo de Bradford
[Proteina] (mg/ml)
Abso
rban
cia 5
95 n
m
1.913.1
%Especie en la proteina
0 0.05 0.1 0.15 0.2 0.25 0.3 0.350
0.1
0.2
0.3
0.4
0.5
0.6
f(x) = 0.13198820509467 ln(x) + 0.634101332527071R² = 0.952103312541384
Metodo de Bradford
[Proteina] (mg/ml)
Abso
rban
cia 5
95 n
m
