Ansan P.O.Box 29, Seoul 425-600, Korea TEL : +82-31-400-6000 FAX : +82-31-408-5820

www.kordi.re.kr

KORDI S&T ● 발행인: 강정극 ● 기획·편집: 성과확산팀 ● 디자인: 모모새 비지니스 ● 발행일: 2010. 1 ● 발행처: 한국해양연구원

KORDI, in quest of the world’s best oceanscience and technology!

This publication includes reviews of outstanding research papers published in Nature in 2009, as

well as summaries of 12 research papers and relevant figures to introduce research paper trends. It

also provides summaries of 16 patents and relevant figures to describe patent trends.

KORDI seeks to share the precious research results it achieved during 2009, enabled by the

strenuous efforts and devotion of its researchers. I hope you take your time to read about the key

research programs and science and technologies of KORDI. I also hope to see this publication

contribute to increased interest and understanding of ocean science among the general public.

January 2010

PresidentKorea Ocean Research & Development Institute

Remarks on 『KORDI S&T Trends for 2009』KANG Jung-Keuk, President of KORDI

Advance into a research institute that presents

answers and solutions for national needs and

help prepares for the future, based on research

and development of world-leading marine

science and technologies

Contents02

Nations all across the globe are continually strengthening research capabilities andincreasing investments in marine science to enhance national competitiveness. They areconcentrating on researches aimed at addressing such global issues as climate change and

environmental pollution, and focus on enabling usage of abundant marine resources, including

marine mineral resources, biological resources, and ocean energy.

To meet the needs of this and prepare for the future era, the Korea Ocean Research &

Development Institute (KORDI) carried out comprehensive marine science research programs aimed

at promoting national interests. KORDI was able to make a number of achievements in 2009, thanks

to such efforts. KORDI put together information on some of its achievements and published 『KORDIS&T Trends』.

With the issuance of this publication, KORDI plans to publicize the excellence of its technologies,

including research papers and patents, and diversify ways of delivering research results and

technology information, thereby providing information on recent achievements and relevant

knowledge, and collecting opinions from readers and users.

0505 0509 0511 05130507

0515 0519 0521 05230517

05270525

0529

39

0533 0535 05370531

Forword

Excellence Article Review

Articles

Patents

History of KORDI

Forword

This publication includes reviews of outstanding research papers published in Nature in 2009, as

well as summaries of 12 research papers and relevant figures to introduce research paper trends. It

also provides summaries of 16 patents and relevant figures to describe patent trends.

KORDI seeks to share the precious research results it achieved during 2009, enabled by the

strenuous efforts and devotion of its researchers. I hope you take your time to read about the key

research programs and science and technologies of KORDI. I also hope to see this publication

contribute to increased interest and understanding of ocean science among the general public.

January 2010

PresidentKorea Ocean Research & Development Institute

Remarks on 『KORDI S&T Trends for 2009』KANG Jung-Keuk, President of KORDI

Advance into a research institute that presents

answers and solutions for national needs and

help prepares for the future, based on research

and development of world-leading marine

science and technologies

Contents02

Nations all across the globe are continually strengthening research capabilities andincreasing investments in marine science to enhance national competitiveness. They areconcentrating on researches aimed at addressing such global issues as climate change and

environmental pollution, and focus on enabling usage of abundant marine resources, including

marine mineral resources, biological resources, and ocean energy.

To meet the needs of this and prepare for the future era, the Korea Ocean Research &

Development Institute (KORDI) carried out comprehensive marine science research programs aimed

at promoting national interests. KORDI was able to make a number of achievements in 2009, thanks

to such efforts. KORDI put together information on some of its achievements and published 『KORDIS&T Trends』.

With the issuance of this publication, KORDI plans to publicize the excellence of its technologies,

including research papers and patents, and diversify ways of delivering research results and

technology information, thereby providing information on recent achievements and relevant

knowledge, and collecting opinions from readers and users.

0505 0509 0511 05130507

0515 0519 0521 05230517

05270525

0529

39

0533 0535 05370531

Forword

Excellence Article Review

Articles

Patents

History of KORDI

Forword

Methods section

The SST analyzed in this study is taken from the

Extended Reconstruction SST version 2 (ERSST.v2) covering

the period of 1854-2007 released by the National Climatic

Data Center 25. In addition, the Climate Prediction Center

(CPC) Merged Analysis of Precipitation (CMAP) data is used

for the period of 1979-2007 26. Atmospheric circulation data

were taken from the National Centers for Environmental

Prediction/National Center for Atmospheric Science

(NCEP/NCAR) reanalysis data27 which use a grid with a

horizontal resolution of 2.5° x 2.5°. In order to define the two

types of El Ni n~o we first collect the years in which the NINO3

SST index during the boreal winter (December-January-

No. 1 January 2010

El Nin~o in achanging climate

Sang-Wook YehClimate Change & Coastal Disaster Research Department¹

swyeh@kordi.re.kr

KORDI S& T

Jong-Seong KugClimate Change & Coastal Disaster Research Department¹

jskug@kordi.re.kr

Excellence Article Review

2009 Macmillan Publishers Limited. All rights reserved 2009 Macmillan Publishers Limited. All rights reserved

NATURE | VOL. 461 | 24 September 2009 Nature

R ecent studies1-5 show different El Niño avor compared to the conventional El Niño which typically exhibits warming in the cold tongue region of the eastern equa-

torial Pacic. Based on recent literature, we look for two

“avors” of El Niño, those with maximum sea surface tempera-

ture (SST) center in the eastern Pacic, and those in the central

Pacic, recently cataloged under the names “Date line El Niño”,

“El Niño Modoki”, “Central Pacic El Niño” and “Warm pool El

Niño” etc., both with distinctly different tropical-midlatitude

teleconnections.

We examine the relative frequency of these two different avors

of El Niño in future climate projections using the Coupled Model

Intercomparison Project phase 3 (CMIP3) multi-model dataset6.

We found that the change in El Niño properties under anthropo-

genic climate change is characterized by more frequent

occurrence of the El Niño with the maximum SST center in the

central Pacic compared to the conventional El Niño, which is

related to a attening of the thermocline in the equatorial Pacic.

Methods section

The SST analyzed in this study is taken from the

Extended Reconstruction SST version 2 (ERSST.v2) covering

the period of 1854-2007 released by the National Climatic

Data Center 25. In addition, the Climate Prediction Center

(CPC) Merged Analysis of Precipitation (CMAP) data is used

for the period of 1979-2007 26. Atmospheric circulation data

were taken from the National Centers for Environmental

Prediction/National Center for Atmospheric Science

(NCEP/NCAR) reanalysis data27 which use a grid with a

horizontal resolution of 2.5° x 2.5°. In order to define the two

types of El Ni n~o we first collect the years in which the NINO3

SST index during the boreal winter (December-January-

No. 1 January 2010

El Nin~o in achanging climate

Sang-Wook YehClimate Change & Coastal Disaster Research Department¹

swyeh@kordi.re.kr

KORDI S& T

Jong-Seong KugClimate Change & Coastal Disaster Research Department¹

jskug@kordi.re.kr

Excellence Article Review

2009 Macmillan Publishers Limited. All rights reserved 2009 Macmillan Publishers Limited. All rights reserved

NATURE | VOL. 461 | 24 September 2009 Nature

R ecent studies1-5 show different El Niño avor compared to the conventional El Niño which typically exhibits warming in the cold tongue region of the eastern equa-

torial Pacic. Based on recent literature, we look for two

“avors” of El Niño, those with maximum sea surface tempera-

ture (SST) center in the eastern Pacic, and those in the central

Pacic, recently cataloged under the names “Date line El Niño”,

“El Niño Modoki”, “Central Pacic El Niño” and “Warm pool El

Niño” etc., both with distinctly different tropical-midlatitude

teleconnections.

We examine the relative frequency of these two different avors

of El Niño in future climate projections using the Coupled Model

Intercomparison Project phase 3 (CMIP3) multi-model dataset6.

We found that the change in El Niño properties under anthropo-

genic climate change is characterized by more frequent

occurrence of the El Niño with the maximum SST center in the

central Pacic compared to the conventional El Niño, which is

related to a attening of the thermocline in the equatorial Pacic.

Enzymes prom Hyperthermophilic / H. Suk Lee

PIV Technique with Bubble Tracers / P. G Paik

Climate Dynamics / J. Ssong Kug

Acoustic Estimates of Japanese Anchovy / D H Kang

3-D Variational Assimilation / Y. H Kim

Flexure Model / C. H kim

Sharing of Skeleton mod for I.P / D. H Mun

Catalytic Enantioselective Enyne RCM Reactions / Y. J Lee

Methane-derived Authigenic Carbonates / Dhongil Lim

Ipid Dynamics of Euphausiids / S. J Ju

ECS Environmen / D. S Kim

Articles

Enzymes prom Hyperthermophilic / H. Suk Lee

PIV Technique with Bubble Tracers / P. G Paik

Climate Dynamics / J. Ssong Kug

Acoustic Estimates of Japanese Anchovy / D H Kang

3-D Variational Assimilation / Y. H Kim

Flexure Model / C. H kim

Sharing of Skeleton mod for I.P / D. H Mun

Catalytic Enantioselective Enyne RCM Reactions / Y. J Lee

Methane-derived Authigenic Carbonates / Dhongil Lim

Ipid Dynamics of Euphausiids / S. J Ju

ECS Environmen / D. S Kim

Articles

Sulfate activation pathway is required for both sulfate assimilation and sulfation processes, and

inorganic pyrophosphatase and 3́ -phosphoadenosine-5́ -phosphate (PAP) phosphatase are important

regulators of the pathway (Figure 1). Based on the genome analysis of the hyperthermophilic archaeon

Thermococcus onnurineus NA1, it was found that the sulfate activation system, ATP sulfurylase and APS

kinase, clustered with two hypothetical genes, TON_1705 and TON_1706 (Figure 2). The gene organization

where four genes are clustered was also found only in Pyrococcus abyssi among all the genomes. By

heterologous expression and biochemical analysis, they were functionally validated to be an inorganic

pyrophosphatase and a PAP phosphatase by exhibiting high substrate specificity (Table 1). This is the first

report to experimentally assess the presence of the soluble pyrophosphatase belonging to the alkaline

phosphatase superfamily. TON_1706-encoded protein was identified to be a unique PAP phosphatase

forming a branch in phylogenetic tree among members of DHH phosphoesterase superfamily (Figure 3).

16 | KORDI S&T

dentification and characterization of inorganicpyrophosphatase and PAP phosphatase from Thermococcus onnurineus NA1.

Articles

H. Suk LeeMarine Biotechnology Research Department●

leeh522@kordi.re.kr

The objective of present study is to investigate propeller wake using PIV technique with bubble type of

tracers, naturally generated by the decrease of the static pressure in a cavitation tunnel. The bubble can be

grown from the nuclei melted in the water tunnel and the size of bubbles is changed by varying the tunnel

pressure. A series of experiments are conducted in the conditions of the uniform and high velocity gradient

flows to find out the characteristics of bubble tracers and compared the measurement results using

bubbles with those using solid particles. Bubbles showed a good trace ability in the region of 15 � Re_S �

75, however, some discrepancies at high velocity gradient region of Re_S ~ 1000. The fitted vorticity

reduction rate would give reference for the prediction in a real flow when bubble tracers are utilized in PIV

measurements of a vortical flow. In addition, the characteristics of bubble slip velocity can provide

information on the vortex core center and the reduction of the Reynolds shear stress caused by bubble’s

deformability.

easurements of high Velocity Gradient Flow Using Bubble Tracers in a Cavitation Tunnel

P. G PaikMarine transportationResearch Department●

ppaik@kordi.re.kr

No. 1 January 2010 | 17

Articles

[Figure 1] Sulfate assimilation pathway. [Figure 2]Organization of the gene cluster involved in sulfateactivation system.

[Figure 3]Phylogenetic relationships of the PAP phosphatase(TON_1706) with the members of DHHphosphoesterase superfamily.

Pyrophosphate 100 �1PAP ND 100

PAPS ND 23ADP 5 �1

3́ -AMP 4 �1ATP 4 �1AMP 1 �1

Polyphosphate �1 �13́ -CMP ND �1Fructose 1,6-bisphosphate ND �1D-myo-Inositol 1-monophosphate ND �1

Pyrophosphatase PAP phosphatase

ActivitySubstrateα

[Table 1] Substrate specificity of the phosphatase activities.

[Figure 1]Experimental set-up for PIVmeasurements

[Figure 2]

High speed camera system

[Figure 3]

Bubble image captured by high speed

camera (Re�106)

[Figure 4]

Particle image using bubble tracers

[Figure 5]

Vorticity contours of propeller

wake measured by bubble tracers

I M

Journal of Bacteriology., 191(10), 3415~3419. 2009 Journal of Fluids Engineering., 131(9), 1301~1310. 2009

Sulfate activation pathway is required for both sulfate assimilation and sulfation processes, and

inorganic pyrophosphatase and 3́ -phosphoadenosine-5́ -phosphate (PAP) phosphatase are important

regulators of the pathway (Figure 1). Based on the genome analysis of the hyperthermophilic archaeon

Thermococcus onnurineus NA1, it was found that the sulfate activation system, ATP sulfurylase and APS

kinase, clustered with two hypothetical genes, TON_1705 and TON_1706 (Figure 2). The gene organization

where four genes are clustered was also found only in Pyrococcus abyssi among all the genomes. By

heterologous expression and biochemical analysis, they were functionally validated to be an inorganic

pyrophosphatase and a PAP phosphatase by exhibiting high substrate specificity (Table 1). This is the first

report to experimentally assess the presence of the soluble pyrophosphatase belonging to the alkaline

phosphatase superfamily. TON_1706-encoded protein was identified to be a unique PAP phosphatase

forming a branch in phylogenetic tree among members of DHH phosphoesterase superfamily (Figure 3).

16 | KORDI S&T

dentification and characterization of inorganicpyrophosphatase and PAP phosphatase from Thermococcus onnurineus NA1.

Articles

H. Suk LeeMarine Biotechnology Research Department●

leeh522@kordi.re.kr

The objective of present study is to investigate propeller wake using PIV technique with bubble type of

tracers, naturally generated by the decrease of the static pressure in a cavitation tunnel. The bubble can be

grown from the nuclei melted in the water tunnel and the size of bubbles is changed by varying the tunnel

pressure. A series of experiments are conducted in the conditions of the uniform and high velocity gradient

flows to find out the characteristics of bubble tracers and compared the measurement results using

bubbles with those using solid particles. Bubbles showed a good trace ability in the region of 15 � Re_S �

75, however, some discrepancies at high velocity gradient region of Re_S ~ 1000. The fitted vorticity

reduction rate would give reference for the prediction in a real flow when bubble tracers are utilized in PIV

measurements of a vortical flow. In addition, the characteristics of bubble slip velocity can provide

information on the vortex core center and the reduction of the Reynolds shear stress caused by bubble’s

deformability.

easurements of high Velocity Gradient Flow Using Bubble Tracers in a Cavitation Tunnel

P. G PaikMarine transportationResearch Department●

ppaik@kordi.re.kr

No. 1 January 2010 | 17

Articles

[Figure 1] Sulfate assimilation pathway. [Figure 2]Organization of the gene cluster involved in sulfateactivation system.

[Figure 3]Phylogenetic relationships of the PAP phosphatase(TON_1706) with the members of DHHphosphoesterase superfamily.

Pyrophosphate 100 �1PAP ND 100

PAPS ND 23ADP 5 �1

3́ -AMP 4 �1ATP 4 �1AMP 1 �1

Polyphosphate �1 �13́ -CMP ND �1Fructose 1,6-bisphosphate ND �1D-myo-Inositol 1-monophosphate ND �1

Pyrophosphatase PAP phosphatase

ActivitySubstrateα

[Table 1] Substrate specificity of the phosphatase activities.

[Figure 1]Experimental set-up for PIVmeasurements

[Figure 2]

High speed camera system

[Figure 3]

Bubble image captured by high speed

camera (Re�106)

[Figure 4]

Particle image using bubble tracers

[Figure 5]

Vorticity contours of propeller

wake measured by bubble tracers

I M

Journal of Bacteriology., 191(10), 3415~3419. 2009 Journal of Fluids Engineering., 131(9), 1301~1310. 2009

18 | KORDI S&T No. 1 January 2010 | 19

[Figure 1] Schematic diagram of syniotic eddyfeedback on low-frequency Flow and the left-handtule. Solid elliptic circles indicate transient synopticcyclonic (red) and anticyclonic (blue) eddies (zc0);blue arrow indicates low-frequency flow. Red(blue)shading indicates positive (negative) eddy vorticcityperturbations (za0) generated by advection of thesynoptic eddies by anomalous low-frequency Flow.Brown arrows indicate eddy-vorticity fluxes.

[Figure 2] The same as Fig. 2 except for AO index

In this study, scale interaction between synoptic eddies and low-frequency flow is investigated. It is

demonstrated here that there is a general rule, the “left-hand rule”, that describes synoptic eddy feedback

onto low-frequency flow. The rule is that low-frequency anomalies systematically stir and deform the

transient eddies in such a way that the irrotational eddy-vorticity fluxes are directed preferentially about 90°

toward the left-hand side of the low-frequency flow; thus this eddy feedback plays a positive role in

maintaining and prolonging low-frequency flow under the stormy atmosphere. Similarly, we show that the

irrotational eddy-temperature and moisture fluxes are also directed preferentially about 90° toward the left-

hand side of the low-frequency flow in the northern hemisphere. The eddy-temperature fluxes and their

vertical structure play a positive role in reinforcing the low-frequency flow in the low levels. The moisture

fluxes play a role in enhancing low-frequency flow by providing eddy-induced moisture convergence and

divergence.

general rule for synoptic-eddy feedback onto low-frequency flow Climate Dynamics 33 (1) 1-17

J. Ssong KugClimateChange&Coastal Disster ResearchDepartment●

jskug@kordi.re.kr

The Japanese anchovy (Engraulis japonicus) is an important species in regard to the fisheries and

ecology of the coastal Northwest Pacific. Measurements of ex situ target strength (TS; dB re 1m2) were

made on live anchovy using 38, 120, and 200 kHz split-beam echosounders. The fish were tethered using

small hooks attached to their mouths. During the acoustic measurements, an underwater video camera

was used to continuously monitor fish behaviour and tilt-angle (θ). Data for 35 individual anchovy ranging

from immature to adult sizes (total lengths, LT = 4.8~12.2cm) were analysed. Least-squares regression fits

of TS vs. log(LT) were: TS38kHz=20·log(LT)-65.8 (r2=0.82), TS120kHz=20·log(LT)-68.4 (r2=0.84), and TS200kHz= 20·

log(LT)-69.1 (r2=0.71).

The LT vs. wet weight (W; g) relationship for these fish was W= 0.0036 LT3.204. The mean θ for anchovy

swimming freely in a large seawater tank was 9.1。(s.d. = 13.18). These ex situ measurements of TS, LT, W,

and θcan be applied to improve acoustic estimates of Japanese anchovy biomass.

x situ target-strength measurementsof Japanese anchovy (Engraulis japonicus) in the coastal Northwest Pacific

D. H KangMarine LivingResources ReserchDepartment●

dhkang@kordi.re.kr

Articles Articles

[Figure 1]Apparatus for acoustic targetstrength experiments of anchovy.

[Figure 2]Various equipments foracousin target strengthmeasurements.

[Figure 3]An example of anchovy’sacousin target strength

[Figure 4]

Functions of acousin target withanchovy’s length for variousfrequencies

A E

Climate Dynamics., 33(1), 1~17. 2009 ICES Journal of Marine Science., 66(6), 1219~1224. 2009

18 | KORDI S&T No. 1 January 2010 | 19

[Figure 1] Schematic diagram of syniotic eddyfeedback on low-frequency Flow and the left-handtule. Solid elliptic circles indicate transient synopticcyclonic (red) and anticyclonic (blue) eddies (zc0);blue arrow indicates low-frequency flow. Red(blue)shading indicates positive (negative) eddy vorticcityperturbations (za0) generated by advection of thesynoptic eddies by anomalous low-frequency Flow.Brown arrows indicate eddy-vorticity fluxes.

[Figure 2] The same as Fig. 2 except for AO index

In this study, scale interaction between synoptic eddies and low-frequency flow is investigated. It is

demonstrated here that there is a general rule, the “left-hand rule”, that describes synoptic eddy feedback

onto low-frequency flow. The rule is that low-frequency anomalies systematically stir and deform the

transient eddies in such a way that the irrotational eddy-vorticity fluxes are directed preferentially about 90°

toward the left-hand side of the low-frequency flow; thus this eddy feedback plays a positive role in

maintaining and prolonging low-frequency flow under the stormy atmosphere. Similarly, we show that the

irrotational eddy-temperature and moisture fluxes are also directed preferentially about 90° toward the left-

hand side of the low-frequency flow in the northern hemisphere. The eddy-temperature fluxes and their

vertical structure play a positive role in reinforcing the low-frequency flow in the low levels. The moisture

fluxes play a role in enhancing low-frequency flow by providing eddy-induced moisture convergence and

divergence.

general rule for synoptic-eddy feedback onto low-frequency flow Climate Dynamics 33 (1) 1-17

J. Ssong KugClimateChange&Coastal Disster ResearchDepartment●

jskug@kordi.re.kr

The Japanese anchovy (Engraulis japonicus) is an important species in regard to the fisheries and

ecology of the coastal Northwest Pacific. Measurements of ex situ target strength (TS; dB re 1m2) were

made on live anchovy using 38, 120, and 200 kHz split-beam echosounders. The fish were tethered using

small hooks attached to their mouths. During the acoustic measurements, an underwater video camera

was used to continuously monitor fish behaviour and tilt-angle (θ). Data for 35 individual anchovy ranging

from immature to adult sizes (total lengths, LT = 4.8~12.2cm) were analysed. Least-squares regression fits

of TS vs. log(LT) were: TS38kHz=20·log(LT)-65.8 (r2=0.82), TS120kHz=20·log(LT)-68.4 (r2=0.84), and TS200kHz= 20·

log(LT)-69.1 (r2=0.71).

The LT vs. wet weight (W; g) relationship for these fish was W= 0.0036 LT3.204. The mean θ for anchovy

swimming freely in a large seawater tank was 9.1。(s.d. = 13.18). These ex situ measurements of TS, LT, W,

and θcan be applied to improve acoustic estimates of Japanese anchovy biomass.

x situ target-strength measurementsof Japanese anchovy (Engraulis japonicus) in the coastal Northwest Pacific

D. H KangMarine LivingResources ReserchDepartment●

dhkang@kordi.re.kr

Articles Articles

[Figure 1]Apparatus for acoustic targetstrength experiments of anchovy.

[Figure 2]Various equipments foracousin target strengthmeasurements.

[Figure 3]An example of anchovy’sacousin target strength

[Figure 4]

Functions of acousin target withanchovy’s length for variousfrequencies

A E

Climate Dynamics., 33(1), 1~17. 2009 ICES Journal of Marine Science., 66(6), 1219~1224. 2009

20 | KORDI S&T No. 1 January 2010 | 21

Reanalyzed products from a MOM3-based East Sea Regional Ocean Model with a 3-dimentionalvariational data assimilation module (DA-ESROM), have been compared with the observed hydrographic and

current datasets in the Ulleung Basin (UB) of the East/Japan Sea (EJS). Satellite-borne sea surface

temperature and sea surface height data, and temperature profiles have been assimilated into the DA-

ESROM. The performance of the DA-ESROM appears to be efficient enough to be used in an operational

ocean forecast system.

Comparing with the results from Mitchell et al. (2005a), the DA-ESROM fairly well simulates the high

variability of the Ulleung Warm Eddy and Dok Cold Eddy as well as the branching of the Tsushima Warm

Current in the UB. The overall root-mean-square error between 100m temperature field reproduced by the

DA-ESROM and the observed 100-dbar temperature field is 2.1℃, and the spatially averaged grid-to-grid

correlation between the two temperature fields is high with a mean value of 0.79 for the inter-comparison

period.

The DA-ESROM reproduces the development of strong southward North Korean Cold Current (NKCC)

in summer consistent with the observational results, which is thought to be an improvement of the previous

numerical models in the EJS. The reanalyzed products show that the NKCC is about 35km wide, and flows

southward along the Korean coast from spring to summer with maximum monthly mean volume transport

of about 0.8 Sv in August-September.

omparison between a reanalyzed product by 3-dimensional variational assimilation technique and observations in the Ulleung Basin of the East/Japan Sea

Y. H KimClimateChange&CoastalDisater Research Department ●

yhkim@kordi.re.kr

[Figure 1]Sea surface height and current (a), and temperature and current at 340m (b) produced by the DA-ESROM on 24 February 1999.

(A) (B)

[Figure 3]Snapshots of 100m temperature fields produced by the DA-ESROM at the same dates in Fig. 2.

[Figure 2]Snapshots of 100-dbar temperature fields from PIES databetween October, 1999 and Juny 2000. Mitchell et al. (2005a).

The isostatic compensation and loading sequence of the Dokdo seamounts on the Ulleung Basin in the

East Sea are studied by a flexure model using gravity and bathymetric data(Figure 1). The Dokdo seamounts

comprise Dokdo (Dok Island), lying as a cluster of emerged small islets surmounting a large submerged

volcanic edifice, and two voluminous tablemounts, Simheungtaek and Isabu(Figure 2). In spite of their

similarity in topographic expression, a large difference of about 50mGal between the observed gravity

anomaly peaks over Dokdo and the Isabu Tablemount implies different amounts of compensation. A

comparison between the observed and calculated gravity anomalies of the seamounts shows that the

flexural compensation investigated in this study predicts well the observed data. The flexure model

suggests that the Dokdo seamounts might be composed of high-density volcanic rocks. Modeling results

show that the flexural rigidity or effective elastic thickness of the lithosphere under Dokdo is stronger or

thicker than that under the Isabu Tablemount(Figure 3). It implies that the age of lithosphere at the time of

loading for Isabu was younger than that for Dokdo. The effective elastic thicknesses associated with the

Dokdo seamounts approximately follow 200-400°C isotherms in the cooling plate model(Figure 4). The

results of this study and the former age dating of Dokdo suggest that the Isabu Tablemount was formed

first, followed by Dokdo after the opening of the East Sea ceased.

lexural isostasy and loading sequence of the Dokdo seamounts on the Ulleung Basin in the East Sea (Sea of Japan)

C. H KimDokdo Research Center●

kimch@kordi.re.kr

Articles Articles

[Figure 1]

Bathymetry of the East Sea. The East Sea is surrounded by Korea, Japan, andRussia. Bathymetric contours are shown in meters. The rectangular box outlined by a solid line representsthe study area.

[Figure 2] (a) Topography of the studyarea. An island and two seamounts areincluded. (b) Topographic profiles of theDokdo seamounts. (c) Free-air anomalymap. Closely spaced gray dots showsurvey tracks. (d) Free-air anomalyprofiles. (e) Magnetic anomaly map. (f)Magnetic anomaly profiles. See Fig. 2a forthe locations of profiles. Contour intervalsare 100m in the topography map, 10 mGalin the free-air anomaly map, and 50nT inthe magnetic anomaly map. Grid intervalsare one minute. Solid contour lines arepositive and dashed contour lines arenegative in the magnetic anomaly map.

[Figure 3]Comparisonbetween the observed and calculated gravityanomaly profiles of theDokdo seamounts. Thecalculated anomalieswere obtained by usinga flexurecompensatedmodel. Upper, middle,and lower profiles are

Dokdo, Simheungtaek Tablemount, and Isabu Tablemount,respectively. See the locations of profiles in the upper rightfigure.

[Figure 4] Te versus age ofseafloor at time of loading.Curves are isotherms for thecooling plate model.Redrawn from Parsons andSclater, 1977; Kruse et al.,1997. Possible seafloor ageat time of loading for Dokdois assumed to be an age

evaluated from the elastic thickness of this study (about5.4km) and the age dating of Dokdo rock samples (Sohn andPark, 1994). Circle with bar denotes Dokdo with error bars at68% confidence level (Sohn and Park, 1994). Possibleseafloor ages at time of loading for the Simheungtaek andthe Isabu tablemounts are presumed to be ages evaluatedfrom the elastic thicknesses of this study (about 1.8km andabout 1.2km, respectively).

C F

JOURNAL OF MARINE SYSTEMS., 78(1), 1~16. 2009 Elsevier., 35(4), 459~468. 2009

20 | KORDI S&T No. 1 January 2010 | 21

Reanalyzed products from a MOM3-based East Sea Regional Ocean Model with a 3-dimentionalvariational data assimilation module (DA-ESROM), have been compared with the observed hydrographic and

current datasets in the Ulleung Basin (UB) of the East/Japan Sea (EJS). Satellite-borne sea surface

temperature and sea surface height data, and temperature profiles have been assimilated into the DA-

ESROM. The performance of the DA-ESROM appears to be efficient enough to be used in an operational

ocean forecast system.

Comparing with the results from Mitchell et al. (2005a), the DA-ESROM fairly well simulates the high

variability of the Ulleung Warm Eddy and Dok Cold Eddy as well as the branching of the Tsushima Warm

Current in the UB. The overall root-mean-square error between 100m temperature field reproduced by the

DA-ESROM and the observed 100-dbar temperature field is 2.1℃, and the spatially averaged grid-to-grid

correlation between the two temperature fields is high with a mean value of 0.79 for the inter-comparison

period.

The DA-ESROM reproduces the development of strong southward North Korean Cold Current (NKCC)

in summer consistent with the observational results, which is thought to be an improvement of the previous

numerical models in the EJS. The reanalyzed products show that the NKCC is about 35km wide, and flows

southward along the Korean coast from spring to summer with maximum monthly mean volume transport

of about 0.8 Sv in August-September.

omparison between a reanalyzed product by 3-dimensional variational assimilation technique and observations in the Ulleung Basin of the East/Japan Sea

Y. H KimClimateChange&CoastalDisater Research Department ●

yhkim@kordi.re.kr

[Figure 1]Sea surface height and current (a), and temperature and current at 340m (b) produced by the DA-ESROM on 24 February 1999.

(A) (B)

[Figure 3]Snapshots of 100m temperature fields produced by the DA-ESROM at the same dates in Fig. 2.

[Figure 2]Snapshots of 100-dbar temperature fields from PIES databetween October, 1999 and Juny 2000. Mitchell et al. (2005a).

The isostatic compensation and loading sequence of the Dokdo seamounts on the Ulleung Basin in the

East Sea are studied by a flexure model using gravity and bathymetric data(Figure 1). The Dokdo seamounts

comprise Dokdo (Dok Island), lying as a cluster of emerged small islets surmounting a large submerged

volcanic edifice, and two voluminous tablemounts, Simheungtaek and Isabu(Figure 2). In spite of their

similarity in topographic expression, a large difference of about 50mGal between the observed gravity

anomaly peaks over Dokdo and the Isabu Tablemount implies different amounts of compensation. A

comparison between the observed and calculated gravity anomalies of the seamounts shows that the

flexural compensation investigated in this study predicts well the observed data. The flexure model

suggests that the Dokdo seamounts might be composed of high-density volcanic rocks. Modeling results

show that the flexural rigidity or effective elastic thickness of the lithosphere under Dokdo is stronger or

thicker than that under the Isabu Tablemount(Figure 3). It implies that the age of lithosphere at the time of

loading for Isabu was younger than that for Dokdo. The effective elastic thicknesses associated with the

Dokdo seamounts approximately follow 200-400°C isotherms in the cooling plate model(Figure 4). The

results of this study and the former age dating of Dokdo suggest that the Isabu Tablemount was formed

first, followed by Dokdo after the opening of the East Sea ceased.

lexural isostasy and loading sequence of the Dokdo seamounts on the Ulleung Basin in the East Sea (Sea of Japan)

C. H KimDokdo Research Center●

kimch@kordi.re.kr

Articles Articles

[Figure 1]

Bathymetry of the East Sea. The East Sea is surrounded by Korea, Japan, andRussia. Bathymetric contours are shown in meters. The rectangular box outlined by a solid line representsthe study area.

[Figure 2] (a) Topography of the studyarea. An island and two seamounts areincluded. (b) Topographic profiles of theDokdo seamounts. (c) Free-air anomalymap. Closely spaced gray dots showsurvey tracks. (d) Free-air anomalyprofiles. (e) Magnetic anomaly map. (f)Magnetic anomaly profiles. See Fig. 2a forthe locations of profiles. Contour intervalsare 100m in the topography map, 10 mGalin the free-air anomaly map, and 50nT inthe magnetic anomaly map. Grid intervalsare one minute. Solid contour lines arepositive and dashed contour lines arenegative in the magnetic anomaly map.

[Figure 3]Comparisonbetween the observed and calculated gravityanomaly profiles of theDokdo seamounts. Thecalculated anomalieswere obtained by usinga flexurecompensatedmodel. Upper, middle,and lower profiles are

Dokdo, Simheungtaek Tablemount, and Isabu Tablemount,respectively. See the locations of profiles in the upper rightfigure.

[Figure 4] Te versus age ofseafloor at time of loading.Curves are isotherms for thecooling plate model.Redrawn from Parsons andSclater, 1977; Kruse et al.,1997. Possible seafloor ageat time of loading for Dokdois assumed to be an age

evaluated from the elastic thickness of this study (about5.4km) and the age dating of Dokdo rock samples (Sohn andPark, 1994). Circle with bar denotes Dokdo with error bars at68% confidence level (Sohn and Park, 1994). Possibleseafloor ages at time of loading for the Simheungtaek andthe Isabu tablemounts are presumed to be ages evaluatedfrom the elastic thicknesses of this study (about 1.8km andabout 1.2km, respectively).

C F

JOURNAL OF MARINE SYSTEMS., 78(1), 1~16. 2009 Elsevier., 35(4), 459~468. 2009

22 | KORDI S&T No. 1 January 2010 | 23

As markets are globalized and competition among companies increases, corporations strive to remain

competitive by focusing their abilities on key parts of a new product, while outsourcing remaining parts to

other companies. In these circumstances, relationships with business partners with regard to product

design are not rigid and vertical, but rather, flexible and horizontal; thus, partners today can be competitors

in the future. Therefore, the intellectual property of a participating company should be protected from other

collaborating companies during the product design process. However, this safeguard should not be an

obstacle to product design collaboration itself. As a solution to this problem, we propose a method to share

a skeleton model among collaborating companies. By using this model, participating companies can share

essential data required for the detail design of those components of a product for which they are

responsible, while ensuring security of their intellectual property. The feasibility of the proposed method

has been demonstrated through experiments in a development scenario involving a tub component of a

washing machine.

rotection of intellectual property based on a skeleton model in product design collaboration

D. H MunMarine Safety&PollutionResearch Department●

dhmun@kordi.re.kr

[Figure 1]Engineering change referring to updated skeleton model data

[Figure 2]Skeleton model-based collaborationamong OEM and parts suppliers

ndo-Selective Enyne Ring-Closing Metathesis Promoted by Stereogenic-at-Mo Monoalkoxide and Monoaryloxide Complexes. Efficient Synthesis of Cyclic Dienes Not Accessible through Reactions with Ru Carbe

Y. J LeeMarine BiotechnologyResearch Department●

yjlee@kordi.re.kr

Articles Articles

P EStereogenic-at-Mo monoalkoxide and monoaryloxide complexes promote enyne ring-closing

metathesis (RCM) reactions, affording the corresponding endo products with high selectivity (typically �98:

� 2 endo:exo). All catalysts can be prepared and used in situ. Five-, six-, and seven-membered rings are

obtained through reactions with enyne substrates that bear all-carbon tethers as well as those that contain

heteroatom substituents. The newly developed catalytic protocols complement the related exo-selective

Ru-catalyzed processes. In cases where Ru-based complexes deliver exo and endo products

nondiscriminately, such as when tetrasubstituted cyclic alkenes are generated, Mo-catalyzed reactions

afford the endo product exclusively. The efficiency of synthesis of N- and O-containing endo diene

heterocycles can be improved significantly through structural modification of Mo catalysts. The modularity

of Mo-based monopyrrolides is thus exploited in the identification of the most effective catalyst variants.

Through alteration of O-based monodentate ligands, catalysts have been identified that promote enyne RCM

with improved efficiency. The structural attributes of three Mo complexes are elucidated through X-ray

crystallography. The first examples of catalytic enantioselective enyne RCM reactions are reported (up to

98:2) enantiomer ratio and �98% endo).

Computer-Aided Design., 41(9), 641~648. 2009 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY., 131(30), 10652~10661. 2009

22 | KORDI S&T No. 1 January 2010 | 23

As markets are globalized and competition among companies increases, corporations strive to remain

competitive by focusing their abilities on key parts of a new product, while outsourcing remaining parts to

other companies. In these circumstances, relationships with business partners with regard to product

design are not rigid and vertical, but rather, flexible and horizontal; thus, partners today can be competitors

in the future. Therefore, the intellectual property of a participating company should be protected from other

collaborating companies during the product design process. However, this safeguard should not be an

obstacle to product design collaboration itself. As a solution to this problem, we propose a method to share

a skeleton model among collaborating companies. By using this model, participating companies can share

essential data required for the detail design of those components of a product for which they are

responsible, while ensuring security of their intellectual property. The feasibility of the proposed method

has been demonstrated through experiments in a development scenario involving a tub component of a

washing machine.

rotection of intellectual property based on a skeleton model in product design collaboration

D. H MunMarine Safety&PollutionResearch Department●

dhmun@kordi.re.kr

[Figure 1]Engineering change referring to updated skeleton model data

[Figure 2]Skeleton model-based collaborationamong OEM and parts suppliers

ndo-Selective Enyne Ring-Closing Metathesis Promoted by Stereogenic-at-Mo Monoalkoxide and Monoaryloxide Complexes. Efficient Synthesis of Cyclic Dienes Not Accessible through Reactions with Ru Carbe

Y. J LeeMarine BiotechnologyResearch Department●

yjlee@kordi.re.kr

Articles Articles

P EStereogenic-at-Mo monoalkoxide and monoaryloxide complexes promote enyne ring-closing

metathesis (RCM) reactions, affording the corresponding endo products with high selectivity (typically �98:

� 2 endo:exo). All catalysts can be prepared and used in situ. Five-, six-, and seven-membered rings are

obtained through reactions with enyne substrates that bear all-carbon tethers as well as those that contain

heteroatom substituents. The newly developed catalytic protocols complement the related exo-selective

Ru-catalyzed processes. In cases where Ru-based complexes deliver exo and endo products

nondiscriminately, such as when tetrasubstituted cyclic alkenes are generated, Mo-catalyzed reactions

afford the endo product exclusively. The efficiency of synthesis of N- and O-containing endo diene

heterocycles can be improved significantly through structural modification of Mo catalysts. The modularity

of Mo-based monopyrrolides is thus exploited in the identification of the most effective catalyst variants.

Through alteration of O-based monodentate ligands, catalysts have been identified that promote enyne RCM

with improved efficiency. The structural attributes of three Mo complexes are elucidated through X-ray

crystallography. The first examples of catalytic enantioselective enyne RCM reactions are reported (up to

98:2) enantiomer ratio and �98% endo).

Computer-Aided Design., 41(9), 641~648. 2009 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY., 131(30), 10652~10661. 2009

24 | KORDI S&T

Photographs showing (a) the gas hydrate sample recovered at the base of core from Ulleung Basin of Korea and (b) the emission of gas from the core sediments.

Authigenic carbonates were sampled in methane-enriched piston core sediments collected from gas

venting sites on the western continental slope of the Ulleung Basin, East Sea. Multidisciplinary

investigations on these carbonates, including the SEM observations and minerlogical-geochemical

compositions, were carried out in order to identify the carbon and oxygen sources and the forming

mechanism of these carbonates. The authigenic carbonates from the study area correspond to semi-

consolidated, compact concretions or nodules ranging from 2 to 9 cm in size. X-ray diffraction and electron

microprobe analyses showed that most of the sampled carbonate concretions were composed of almost

purely authigenic high-Mg calcite (10.7~14.3 mol% MgCO3). Characteristically, microbial structures such as

filaments and rods, which were probably associated with the authigenic minerals, The carbonates were

strongly depleted in δ13C (-33.85 to -39.53‰ Peedee Belemnite [PDB]) and were enriched in δ18O (5.16 to 5.60

‰ PDB), indicating that the primary source of carbon is mainly derived from the anaerobic oxidation of

methane. Such methane probably originated from the destabilization of the underlying gas hydrates as

strongly supporting from the enriched 18O levels. Furthermore, the strongly depleted δ13C values (-60.7 to -

61.6‰ PDB) of the sediment void gases demonstrate that the majority of the gas venting at the Ulleung

Basin is microbial methane by CO2 reduction. This study provides another example for the formation

mechanism of methane-derived authigenic carbonates associated with gas hydrate decomposition in gas-

seeping pockmark environments.

ethane-derived authigenic carbonates from the Ulleung Basin sediments, East Sea of Korea

Dhongil LimSouth Sea EnvironmentResearch Department●

oceanlim@kordi.re.kr

Authigenic carbonate concretion samples recovered in different depth ofthe core and X-ray diffraction patterns of these carbonate concretionsamples. The carbonate concretions is composed of high-Mg calcite(HMC). Average MgCO3 contents (13.0 mol%) in the carbonate samples arecomparable to those of methane-derived calcites from the easternMediterranean Sea, the northern Gulf of Mexico, Baffin Bay, Monterey Bay.

Diversity of microbial structures preserved in authigenic carbonates, SEMmicrophotograhs. (a), (b) and (c) typical biological fossils, (d) biofilm maybealgal mat), and (e) and (f) microbial activities (worm tube). Microbialstructures and/or remnants of worm tubes within the carbonate matrixmay represent useful indicators of methane seepage related to gashydrate decomposition.

SEM photographs showing (a) the compact and rough surface of

authigenic carbonate, (b) the roughly equigranular, anhedral calcite

crystals and various biological impurities, and (c) the elongated aragonite

crystals.

Relationship between the ratio of C1/C2 +hydrocarbonsandstablecarbonisotopiccomposition(δ13C) of methane inhydrate-bound gases from the global dataset subdivided into areas of highgas flux (HGF), low gas flux (LGF), and hydrate gas accumulations (HGF).Note that molecular and stable isotopic compositions of methane fromcurrent study fall in the field of microbial gas. The field of microbial,thermogenic and mixed gases are defined after Whiticar (1999) and basicframe of the figure and data are from Milkov (2005).

No. 1 January 2010 | 25

Articles Articles

(A) (B)

M

CONTINENTAL SHELF RESEARCH., 29(13), 1588~1596. 2009

24 | KORDI S&T

Photographs showing (a) the gas hydrate sample recovered at the base of core from Ulleung Basin of Korea and (b) the emission of gas from the core sediments.

Authigenic carbonates were sampled in methane-enriched piston core sediments collected from gas

venting sites on the western continental slope of the Ulleung Basin, East Sea. Multidisciplinary

investigations on these carbonates, including the SEM observations and minerlogical-geochemical

compositions, were carried out in order to identify the carbon and oxygen sources and the forming

mechanism of these carbonates. The authigenic carbonates from the study area correspond to semi-

consolidated, compact concretions or nodules ranging from 2 to 9 cm in size. X-ray diffraction and electron

microprobe analyses showed that most of the sampled carbonate concretions were composed of almost

purely authigenic high-Mg calcite (10.7~14.3 mol% MgCO3). Characteristically, microbial structures such as

filaments and rods, which were probably associated with the authigenic minerals, The carbonates were

strongly depleted in δ13C (-33.85 to -39.53‰ Peedee Belemnite [PDB]) and were enriched in δ18O (5.16 to 5.60

‰ PDB), indicating that the primary source of carbon is mainly derived from the anaerobic oxidation of

methane. Such methane probably originated from the destabilization of the underlying gas hydrates as

strongly supporting from the enriched 18O levels. Furthermore, the strongly depleted δ13C values (-60.7 to -

61.6‰ PDB) of the sediment void gases demonstrate that the majority of the gas venting at the Ulleung

Basin is microbial methane by CO2 reduction. This study provides another example for the formation

mechanism of methane-derived authigenic carbonates associated with gas hydrate decomposition in gas-

seeping pockmark environments.

ethane-derived authigenic carbonates from the Ulleung Basin sediments, East Sea of Korea

Dhongil LimSouth Sea EnvironmentResearch Department●

oceanlim@kordi.re.kr

Authigenic carbonate concretion samples recovered in different depth ofthe core and X-ray diffraction patterns of these carbonate concretionsamples. The carbonate concretions is composed of high-Mg calcite(HMC). Average MgCO3 contents (13.0 mol%) in the carbonate samples arecomparable to those of methane-derived calcites from the easternMediterranean Sea, the northern Gulf of Mexico, Baffin Bay, Monterey Bay.

Diversity of microbial structures preserved in authigenic carbonates, SEMmicrophotograhs. (a), (b) and (c) typical biological fossils, (d) biofilm maybealgal mat), and (e) and (f) microbial activities (worm tube). Microbialstructures and/or remnants of worm tubes within the carbonate matrixmay represent useful indicators of methane seepage related to gashydrate decomposition.

SEM photographs showing (a) the compact and rough surface of

authigenic carbonate, (b) the roughly equigranular, anhedral calcite

crystals and various biological impurities, and (c) the elongated aragonite

crystals.

Relationship between the ratio of C1/C2 +hydrocarbonsandstablecarbonisotopiccomposition(δ13C) of methane inhydrate-bound gases from the global dataset subdivided into areas of highgas flux (HGF), low gas flux (LGF), and hydrate gas accumulations (HGF).Note that molecular and stable isotopic compositions of methane fromcurrent study fall in the field of microbial gas. The field of microbial,thermogenic and mixed gases are defined after Whiticar (1999) and basicframe of the figure and data are from Milkov (2005).

No. 1 January 2010 | 25

Articles Articles

(A) (B)

M

CONTINENTAL SHELF RESEARCH., 29(13), 1588~1596. 2009

26 | KORDI S&T

To better understand the feeding and reproductive ecology of euphausiids (krill) in different oceanenvironments, lipid classes and individual lipid components of four different species of euphausiids from

Northeast Pacific (temperate species) and Southern Ocean (Antarctic species) were analyzed in animals

from multiple life stages and seasons. The dominant krill species in the Northeast Pacific, Euphausia

pacifica and Thysanoessa spinifera, were compared to the two major Antarctic species, Euphausia superba

and E. crystallorophias. Analysis comprised total lipid and lipid classes together with individual fatty acid

and sterol composition in adults, juveniles, and larvae. Antarctic krill had much higher lipid content than

their temperate relatives (10-50 and 5-20% of dry mass for Antarctic and temperate species, respectively)

with significant seasonal variations observed. Phospholipids were the dominant lipid class in both

temperate krill species, while neutral storage lipids (wax esters and triacylglycerols for E. crystallorophias

and E. superba, respectively) were the major lipid class in Antarctic krill and accounted for up to 40% of the

total lipid content. Important fatty acids, specifically 16:0, 18:1w9, 20:5w3, and 22:6w3, were detected in all

four krill species, with minor differences between species and seasons. Detailed lipid profiles suggest that

krill alter their lipid composition with life stage and season. In particular, larval Antarctic krill appear to

utilize alternate food resources (i.e., sea-ice associated organisms) during austral winter in contrast to

juveniles and adults (i.e., seston and copepods). Lipid dynamics of krill

from both systems appear closely linked to their life cycle and

environmental conditions including food availability, and can provide a

more complete comparative ecology of euphausiids in these

environmentally distinct systems.

omparative lipid dynamics of euphausiids from theAntarctic and Northeast Pacific Oceans

S.J JuDeep-sea&MarineGeoresources ReserchDepartment ●

sjju@kordi.re.kr

[Figure 1] Seasonal variation of total lipid content (in percent of drymass, DM) among investigated krill speciesand life stages (A-Euphausia pacifica, B-Thysanoessa spinifera, C- Euphausiasuperba, and D-Euphausia cystallorophias ). Filled symbols withstandard deviations are comparative measures of Antarctic krill inspring and summer months from Hagen et al. (2001) and Kattnerand Hagen (1998). Gray shaded areas indicate the winter season.

[Figure 2] The relative composition of major lipid classes (aspercent of dry mass, DM) and the increments of major lipidclasses with increasing total lipid content (in percent of drymass, DM) in investigated krill species (A- Euphausia pacifica, B-Thysanoessa spinifera, C- Euphausia superba, and D- Euphausiacystallorophias ).

patial and temporal variations in nutrient andchlorophyll-a concentrations in the northern EastChina Sea surrounding Cheju Island

D.S KimClimateChange&CoastalDisaster ReserchDepartment●

dkim@kordi.re.kr

Articles Articles

C S

No. 1 January 2010 | 27

Nutrients, chlorophyll-a (Chl-a), and environmental conditions were extensively investigated in the

northern East China Sea (ECS) near Cheju Island during five research cruises from 2003 to 2007. In the

eastern part of the study area, surface waters were characterized only by the Tsushima Current Water

(TCW) during all five cruises. However, the western surface waters changed with season and were

characterized by Yellow Sea Cold Water (YSCW) in spring, Changjiang Diluted Water (CDW) in summer, and

Yellow Sea Mixed Water (YSMW) in autumn. In spring and autumn, relatively high concentrations of nitrate

and phosphate were observed in the surface waters in the western part of the study area, where vertical

mixing brought large supplies of nutrients from deep waters. Changes in wind direction occasionally varied

the inflow of the Changjiang plume in summer, clearly causing the annual variation in surface nitrate and

phosphate concentrations in summer. In summer, the surface distribution of nitrate and phosphate did not

coincide with that of silicate in the study area, which probably resulted from the significant drop in the Si:N

ratio in the Changjiang plume since construction of the Three Gorges Dam (TGD). Despite large temporal

and spatial variations in surface Chl-a concentrations, depth-integrated Chl-a concentrations exhibited

little variation temporally and spatially. In the study area, surface Chl-a concentration did not well reflect

the standing stocks of phytoplankton. The vertical distribution of Chl-a also showed large temporal and

spatial variations, and the main factor controlling the vertical distribution of Chl-a in summer was the

availability of nitrate. The thermohaline Front may play an important role for accumulation of phytoplankton

biomass in spring and autumn.

MARINE BIOLOGY., 156(7), 1459~1473. 2009 CONTINENTAL SHELF RESEARCH., 29(6), 1426~1436. 2009

26 | KORDI S&T

To better understand the feeding and reproductive ecology of euphausiids (krill) in different oceanenvironments, lipid classes and individual lipid components of four different species of euphausiids from

Northeast Pacific (temperate species) and Southern Ocean (Antarctic species) were analyzed in animals

from multiple life stages and seasons. The dominant krill species in the Northeast Pacific, Euphausia

pacifica and Thysanoessa spinifera, were compared to the two major Antarctic species, Euphausia superba

and E. crystallorophias. Analysis comprised total lipid and lipid classes together with individual fatty acid

and sterol composition in adults, juveniles, and larvae. Antarctic krill had much higher lipid content than

their temperate relatives (10-50 and 5-20% of dry mass for Antarctic and temperate species, respectively)

with significant seasonal variations observed. Phospholipids were the dominant lipid class in both

temperate krill species, while neutral storage lipids (wax esters and triacylglycerols for E. crystallorophias

and E. superba, respectively) were the major lipid class in Antarctic krill and accounted for up to 40% of the

total lipid content. Important fatty acids, specifically 16:0, 18:1w9, 20:5w3, and 22:6w3, were detected in all

four krill species, with minor differences between species and seasons. Detailed lipid profiles suggest that

krill alter their lipid composition with life stage and season. In particular, larval Antarctic krill appear to

utilize alternate food resources (i.e., sea-ice associated organisms) during austral winter in contrast to

juveniles and adults (i.e., seston and copepods). Lipid dynamics of krill

from both systems appear closely linked to their life cycle and

environmental conditions including food availability, and can provide a

more complete comparative ecology of euphausiids in these

environmentally distinct systems.

omparative lipid dynamics of euphausiids from theAntarctic and Northeast Pacific Oceans

S.J JuDeep-sea&MarineGeoresources ReserchDepartment ●

sjju@kordi.re.kr

[Figure 1] Seasonal variation of total lipid content (in percent of drymass, DM) among investigated krill speciesand life stages (A-Euphausia pacifica, B-Thysanoessa spinifera, C- Euphausiasuperba, and D-Euphausia cystallorophias ). Filled symbols withstandard deviations are comparative measures of Antarctic krill inspring and summer months from Hagen et al. (2001) and Kattnerand Hagen (1998). Gray shaded areas indicate the winter season.

[Figure 2] The relative composition of major lipid classes (aspercent of dry mass, DM) and the increments of major lipidclasses with increasing total lipid content (in percent of drymass, DM) in investigated krill species (A- Euphausia pacifica, B-Thysanoessa spinifera, C- Euphausia superba, and D- Euphausiacystallorophias ).

patial and temporal variations in nutrient andchlorophyll-a concentrations in the northern EastChina Sea surrounding Cheju Island

D.S KimClimateChange&CoastalDisaster ReserchDepartment●

dkim@kordi.re.kr

Articles Articles

C S

No. 1 January 2010 | 27

Nutrients, chlorophyll-a (Chl-a), and environmental conditions were extensively investigated in the

northern East China Sea (ECS) near Cheju Island during five research cruises from 2003 to 2007. In the

eastern part of the study area, surface waters were characterized only by the Tsushima Current Water

(TCW) during all five cruises. However, the western surface waters changed with season and were

characterized by Yellow Sea Cold Water (YSCW) in spring, Changjiang Diluted Water (CDW) in summer, and

Yellow Sea Mixed Water (YSMW) in autumn. In spring and autumn, relatively high concentrations of nitrate

and phosphate were observed in the surface waters in the western part of the study area, where vertical

mixing brought large supplies of nutrients from deep waters. Changes in wind direction occasionally varied

the inflow of the Changjiang plume in summer, clearly causing the annual variation in surface nitrate and

phosphate concentrations in summer. In summer, the surface distribution of nitrate and phosphate did not

coincide with that of silicate in the study area, which probably resulted from the significant drop in the Si:N

ratio in the Changjiang plume since construction of the Three Gorges Dam (TGD). Despite large temporal

and spatial variations in surface Chl-a concentrations, depth-integrated Chl-a concentrations exhibited

little variation temporally and spatially. In the study area, surface Chl-a concentration did not well reflect

the standing stocks of phytoplankton. The vertical distribution of Chl-a also showed large temporal and

spatial variations, and the main factor controlling the vertical distribution of Chl-a in summer was the

availability of nitrate. The thermohaline Front may play an important role for accumulation of phytoplankton

biomass in spring and autumn.

MARINE BIOLOGY., 156(7), 1459~1473. 2009 CONTINENTAL SHELF RESEARCH., 29(6), 1426~1436. 2009

Artifcial Hibernation / W. Su Kim

BIO-Ethanol / D. H Kang

Coding Gene / Yun. H Lee

Beta-1, 3-Glucanase From / Yun. H Lee

Epoxide Hydlrolasf / S. J Kim

Helical Turbine / Jin. S Park

Bromophenols Compound / H. S Lee

Pharmaceutical Compositions for Anticancer / H. J Shin

Underwater Image Taking Apparatus / Y. K Lim

Overflow Wave Generater / S. H Shin

Management System for Spare Parts of a ship / P. G Paik

Wake Measurement System / J. W An

Personal Water Craft / K. Sprop Kim

Apparatus For The Fish-Gear Survey / C. G Kang

WIG Ship / Y. Sik Kim

breakwater / S. Y Hong

Patents

Artifcial Hibernation / W. Su Kim

BIO-Ethanol / D. H Kang

Coding Gene / Yun. H Lee

Beta-1, 3-Glucanase From / Yun. H Lee

Epoxide Hydlrolasf / S. J Kim

Helical Turbine / Jin. S Park

Bromophenols Compound / H. S Lee

Pharmaceutical Compositions for Anticancer / H. J Shin

Underwater Image Taking Apparatus / Y. K Lim

Overflow Wave Generater / S. H Shin

Management System for Spare Parts of a ship / P. G Paik

Wake Measurement System / J. W An

Personal Water Craft / K. Sprop Kim

Apparatus For The Fish-Gear Survey / C. G Kang

WIG Ship / Y. Sik Kim

breakwater / S. Y Hong

Patents

No. 1 January 2010 | 31

● 해양생물의 인공동면 유도 장치에 대한 것으로, 특히 해양생물을 포함

하는 해수의 온도를 단계적으로 낮추면서, 이렇게 낮아진 각 단계의 온

도 유지 시간을 단계적으로 확장하는 것임

● 즉, 해수의 온도를 단계적으로 낮추면서, 상기 낮아진 각 단계의 온도마

다 해수의 수온을 일정한 시간 동안 유지시키되, 상기 낮아진 각 단계의

온도에서 유지시키는 일정한 시간을 해양생물이 소비하는 산소소비량의

변화가 크게 줄어들거나 내인성 생체리듬이 정지되어 산소소비량의 변

화가 거의 없는 시점까지 단계적으로 증가시킨 뒤, 이를 기점으로 상기

각 단계에서 해수의 수온을 일정하게 유지하는 시간을 단계적으로 줄

임으로써, 해양생물의 생존율을 장시간 유지시킬 수 있는 효과가 있음

● 어류의 생체리듬을 이용하여 인위적으로 동면을 유도한 후 무수(無水)

운반에 따른 운송비 절감 및 신개념 유통·물류 시스템 구축

● 해조류로부터 간단한 공정으로 고압 액화 추출물, 즉, 글루코오스 추출

물을 최고 수율로 수득할 수 있으며, 발효시간이 단축되어 바이오에탄올

의 생산수율을 최적화 시킬 수 있는 장점이 있음

● 기존의 공정에서 제기되는 황산과 같은 화학물질 사용 및 추출 후 부산

물 처리, 발효억제 물질에 의한 낮은 수율, 복잡한 공정, 환경오염 물질

의 배출 및 첨가물 및 온도로 인한 성분의 변성의 문제로부터 자유로운

장점이 있음

● 또한, 이상 증식하여 환경 문제로 대두되는 해조류로부터 바이오에탄올

을 추출하게 된다면 폐기물로부터의 부가가치 창출이라는 경제적이고

친환경적인 효과가 있음

● 남극톡토기(Cryptopygus antarcticus)으로부터 분리한 저온활성 및

내산성 베타(beta)-1, 4-만난아제(mannanase) 효소를 코딩하는 유전

자, 유전자를 포함하는 발현벡터, 발현벡터에 의해 형질전환된 형질전환

체 및 이로부터 생산되는 상기 효소의 제조방법을 제공함

● 유래의 베타-1, 4-D-만난아제 효소는 저온에서 그 활성을 유지할 수

있으며, 산성조건에서도 활성이 높기 때문에 저온공정이 필요한 식품,

제지 및 발효 산업뿐 아니라 식품첨가물 및 사료첨가물로서 유용하게

이용될 수 있음

인공동면 유도방법에 관한 모식도

소형 인공동면 유도장치

압력을 500 MPa에서 1000 MPa까지 상승시켜 해조류를 액화 시키는 방법

남극톡토기 (Cryptopygus antarcticus)로부터 유래되며, pH 2.5 내지 4

이하의 산성조건에서 안정적이며, 온도 범위 0 내지 35℃ 이하에서 효소

활성을 유지하며, 및 Ca 2+ , Mg 2+ , Cu 2+ 및 Zn 2+ 로 이루어진

군으로부터 선택된 1종 이상의 금속이온에서 적어도 150% 이상의 효소활

성을 나타내는 서열번호 2의 아미노산 서열로 이루어지는 저온활성 및 내

산성 베타-1,4-D-만난아제 단백질

30 | KORDI S&T

● 남극톡토기로부터 분리한 베타-1,3-글루카나아제는 베타-1,3-글루칸인

라미나린을 올리고당화하여 섭취율이 높은 기능성 탄수화물로 전환시키

거나 당생물학 연구분야에서 탄수화물의 특정부위만을 선택적으로 절단

하는 효소로 이용할 수 있고, 나아가 전분 분해공정, 식품, 음료, 사료

및 섬유 산업 등에 유용하게 활용될 수 있음

남극톡토기 (Cryptopygus antarcticus)로부터 유래되며, pH 4 내지 10

이하의 다양한 pH조건에서 안정적이며, 온도 범위 0 내지 60℃ 이하에서

효소활성을 나타내는, 서열번호 3의 아미노산 서열로 구성되고 베타-1,3-

글루칸 분해활성을 갖는 베타-1,3-글루카나아제 단백질

특허등록번호 10-740457 (‘07.07.11) / 10-768475 (‘07.10.12)몽골 3247 (‘09.06.20) / 일본 4332206 (‘09.06.26)일본 4283325 (‘09.03.27)PCT/KR2007/001092('07.03.06) 20개국 출원

발명자 : 김완수

인공동면유도기술및운송기술및그장치METHOD AND APPARATUS FOR INDUCING ARTIFICIAL HIBERNATION OF MARINE ANIMAL

특허등록번호 : 10-908425(‘09.07.13), PCT/KR2008/007489 (‘08.12.17) 2개국 출원

발명자 : 강도형, 이현용, 한재건, 박흥식, 이희승, 강래선

해조류를이용한바이오에탄올제조용고압액화추출물및이의제조방법LIQUEFIED EXTRACT OF MARINE ALGAE FOR PRODUCING BIO-ETHANOL UNDER HIGH PRESSURE AND METHOD FOR PRODUCING THE SAME

특허등록번호 : 10-762410 (‘07.09.20)

발명자 : 이윤호, 김충곤, 송정민

저온활성및내산성베타-１，４－Ｄ－만난아제, 이를코딩하는유전자, 및이의용도COLD-ACTIVE, ACIDIC BETA-1,4-D-MANNANASE, CODINGGENE, AND USE THEREO

특허등록번호 : 10-913233 (‘09.08.13)

발명자 : 이윤호, 송정민, 김충곤

남극톡토기유래의베타-1,3-글루카나아제, 이를코딩하는유전자및이의용도BETA-1,3-GLUCANASE FROM CRYPTOPYGUSANTARCTICUS, GENEENCODING THE SAME, ANDUSE THEREOF

Patents Patents

No. 1 January 2010 | 31

● 해양생물의 인공동면 유도 장치에 대한 것으로, 특히 해양생물을 포함

하는 해수의 온도를 단계적으로 낮추면서, 이렇게 낮아진 각 단계의 온

도 유지 시간을 단계적으로 확장하는 것임

● 즉, 해수의 온도를 단계적으로 낮추면서, 상기 낮아진 각 단계의 온도마

다 해수의 수온을 일정한 시간 동안 유지시키되, 상기 낮아진 각 단계의

온도에서 유지시키는 일정한 시간을 해양생물이 소비하는 산소소비량의

변화가 크게 줄어들거나 내인성 생체리듬이 정지되어 산소소비량의 변

화가 거의 없는 시점까지 단계적으로 증가시킨 뒤, 이를 기점으로 상기

각 단계에서 해수의 수온을 일정하게 유지하는 시간을 단계적으로 줄

임으로써, 해양생물의 생존율을 장시간 유지시킬 수 있는 효과가 있음

● 어류의 생체리듬을 이용하여 인위적으로 동면을 유도한 후 무수(無水)

운반에 따른 운송비 절감 및 신개념 유통·물류 시스템 구축

● 해조류로부터 간단한 공정으로 고압 액화 추출물, 즉, 글루코오스 추출

물을 최고 수율로 수득할 수 있으며, 발효시간이 단축되어 바이오에탄올

의 생산수율을 최적화 시킬 수 있는 장점이 있음

● 기존의 공정에서 제기되는 황산과 같은 화학물질 사용 및 추출 후 부산

물 처리, 발효억제 물질에 의한 낮은 수율, 복잡한 공정, 환경오염 물질

의 배출 및 첨가물 및 온도로 인한 성분의 변성의 문제로부터 자유로운

장점이 있음

● 또한, 이상 증식하여 환경 문제로 대두되는 해조류로부터 바이오에탄올

을 추출하게 된다면 폐기물로부터의 부가가치 창출이라는 경제적이고

친환경적인 효과가 있음

● 남극톡토기(Cryptopygus antarcticus)으로부터 분리한 저온활성 및

내산성 베타(beta)-1, 4-만난아제(mannanase) 효소를 코딩하는 유전

자, 유전자를 포함하는 발현벡터, 발현벡터에 의해 형질전환된 형질전환

체 및 이로부터 생산되는 상기 효소의 제조방법을 제공함

● 유래의 베타-1, 4-D-만난아제 효소는 저온에서 그 활성을 유지할 수

있으며, 산성조건에서도 활성이 높기 때문에 저온공정이 필요한 식품,

제지 및 발효 산업뿐 아니라 식품첨가물 및 사료첨가물로서 유용하게

이용될 수 있음

인공동면 유도방법에 관한 모식도

소형 인공동면 유도장치

압력을 500 MPa에서 1000 MPa까지 상승시켜 해조류를 액화 시키는 방법

남극톡토기 (Cryptopygus antarcticus)로부터 유래되며, pH 2.5 내지 4

이하의 산성조건에서 안정적이며, 온도 범위 0 내지 35℃ 이하에서 효소

활성을 유지하며, 및 Ca 2+ , Mg 2+ , Cu 2+ 및 Zn 2+ 로 이루어진

군으로부터 선택된 1종 이상의 금속이온에서 적어도 150% 이상의 효소활

성을 나타내는 서열번호 2의 아미노산 서열로 이루어지는 저온활성 및 내

산성 베타-1,4-D-만난아제 단백질

30 | KORDI S&T

● 남극톡토기로부터 분리한 베타-1,3-글루카나아제는 베타-1,3-글루칸인

라미나린을 올리고당화하여 섭취율이 높은 기능성 탄수화물로 전환시키

거나 당생물학 연구분야에서 탄수화물의 특정부위만을 선택적으로 절단

하는 효소로 이용할 수 있고, 나아가 전분 분해공정, 식품, 음료, 사료

및 섬유 산업 등에 유용하게 활용될 수 있음

남극톡토기 (Cryptopygus antarcticus)로부터 유래되며, pH 4 내지 10

이하의 다양한 pH조건에서 안정적이며, 온도 범위 0 내지 60℃ 이하에서

효소활성을 나타내는, 서열번호 3의 아미노산 서열로 구성되고 베타-1,3-

글루칸 분해활성을 갖는 베타-1,3-글루카나아제 단백질

특허등록번호 10-740457 (‘07.07.11) / 10-768475 (‘07.10.12)몽골 3247 (‘09.06.20) / 일본 4332206 (‘09.06.26)일본 4283325 (‘09.03.27)PCT/KR2007/001092('07.03.06) 20개국 출원

발명자 : 김완수

인공동면유도기술및운송기술및그장치METHOD AND APPARATUS FOR INDUCING ARTIFICIAL HIBERNATION OF MARINE ANIMAL

특허등록번호 : 10-908425(‘09.07.13), PCT/KR2008/007489 (‘08.12.17) 2개국 출원

발명자 : 강도형, 이현용, 한재건, 박흥식, 이희승, 강래선

해조류를이용한바이오에탄올제조용고압액화추출물및이의제조방법LIQUEFIED EXTRACT OF MARINE ALGAE FOR PRODUCING BIO-ETHANOL UNDER HIGH PRESSURE AND METHOD FOR PRODUCING THE SAME

특허등록번호 : 10-762410 (‘07.09.20)

발명자 : 이윤호, 김충곤, 송정민

저온활성및내산성베타-１，４－Ｄ－만난아제, 이를코딩하는유전자, 및이의용도COLD-ACTIVE, ACIDIC BETA-1,4-D-MANNANASE, CODINGGENE, AND USE THEREO

특허등록번호 : 10-913233 (‘09.08.13)

발명자 : 이윤호, 송정민, 김충곤

남극톡토기유래의베타-1,3-글루카나아제, 이를코딩하는유전자및이의용도BETA-1,3-GLUCANASE FROM CRYPTOPYGUSANTARCTICUS, GENEENCODING THE SAME, ANDUSE THEREOF

Patents Patents

● 헬리컬 터빈에 의해 회전력이 발생되므로 조력댐이 필요한 종래 구

조에 비하여 건설비용이 절감됨과 동시에 환경오염이 방지되는 효과

가 있음

● 전기를 생성하기 위해 동기발전기가 사용되어 설비비가 절감되며,

유체커플링이 장착되어 동기발전기의 과부하를 방지하는 구조로 형

성되어 기능이 향상되는 효과가 있음

● 또한, 헬리컬 터빈에 보조 지지부재와 보조 블레이드를 추가하여 회

전효율을 향상시키는 효과가 있음

● 신규 브로모페놀계 화합물 및 이를 함유하는 항균 조성물은 종래에

보고되지 아니한 신규한 화합물로, 우수한 항균 활성을 가지므로, 기

존에 보고된 항생제를 대체할 수 있어 기존 항생제에 대한 내성을

가진 세균에 의한 질병의 치료 또는 예방에 널리 사용될 수 있음

● 신규한 브로모페놀계 화합물을 제조하는 방법은 간단하여, 기존의

소량의 화합물만을 수득하던 홍조류로부터의 추출방법과 달리 신규

한 화합물을 경제적으로 대량 생산할 수 있는 방법이므로 산업적 효

과가 매우 크다고 볼 수 있음

32 | KORDI S&T No. 1 January 2010 | 33

4 : 동기발전기

5 : 유체커플링

6 : 증속기

20 : 프레임

30 : 헬리컬 터빈

유체의 일방향 또는 다방향 흐름에 대하여 연속적인 회전력을 발생시

킬 수 있도록 프레임에 회전가능하게 설치되는 헬리컬 터빈; 상기 헬

리컬 터빈의 회전을 발전에 필요한 회전속도로 증가시키는 증속기; 상

기 유체의 흐름이 일시적으로 빨라져 상기 증속기를 거친 회전속도가

필요이상으로 속도로 회전되는 것을 방지하는 유체커플링; 및 상기

유체 커플링의 회전속도를 전달받아 전기를 발생시키는 동기발전기;

를 포함하는 것을 특징으로 하는 헬리컬 터빈 발전시스템

에리스로박터 리토랄리스(Erythrobacter Iitoralis) 균주로부터 분리 및 정

제되고, SEQ ID NO : 13의 아미노산 서열을 갖는 광학선택적 에폭사이드

가수분해효소 단백질

아세트산(acetic acid, AcOH)과 디클로로메탄(CH2Cl2) 하에서 2,2'-

디하이드록시디페닐메탄(2,2'-dihydroxydiphenylmethane)과 브롬

(Br2)을 반응시켜, 화학식 1의 구조를 갖는 브로모페놀계 화합물을 제

조하는 단계를 포함하는 것을 특징으로 하는 브로모페놀계 화합물의

제조방법

(화학식 1)상기 식 중, 은 H 또는 Br일 수 있다

● 에리스로박터(Erythrobacter) 종, 스핑고피식(Sphingophyxis) 종,

노보스핑고비움(Novosphingo bium) 종 및 로도박터레일즈

(Rhodobacterales) 속 균주로부터 분리 및 정제된 광학선택적

가수분해효소 활성을 가진 단백질은 약학산업에서 약리활성이 있는

광학순도 에폭사이드를 고효율로 생합성하는데 유용하게 사용될

수 있음

● 방선균(actinomycetes) 스트렙토마이세스 속(Streptomyces sp.)에

속하는 균주의 배양물로부터 분리된 분획물을 유효성분으로 함유하는

항암용 약학 조성물 및 이것의 추출방법을 제공함

● 방선균 스트렙토마이세스 속 균주와 이것의 배양물 및 배양여과액으

로부터 얻어지는 항암물질을 이용하여 인체의 백혈병과 같은 암세

포에 대한 항암활성을 가지는 항암제 조성물을 제공함

MDA-MB-231(유방암) 7.5 0.8

HCT 15(결장암) 7.8 0.9

PC-3(전립선암) 3.2 0.7

NCI-H23(폐암) 3.5 0.5

ACHN(안암) 4.7 0.6

LOX-IMVI(피부암) 7.4 0.5

K-562(백혈병) 8.6 0.3

암세포주방선균유래항암물질 Adriamycin

IC50 (㎍/㎖) IC50 (㎍/㎖)

방선균(actinomycetes) 스트렙토마이세스 속(Streptomyces sp.)에

속하는 균주의 배양물로부터 분리된 분획물을 유효성분으로 함유하며,

아래의 성질을 보유하는 것을 특징으로 하는 방선균 분획물을 유효성

분으로 함유하는 항암용 약학 조성물. -분자식 : C8H9NO2 -분자량 :

151 -성상 : 무색의 무정형 물질로 실온에서 안정 -용해도 : 메탄올,

에탄올, 아세토나이트릴, 프로판올, 아세톤, 다이클로로메탄, 클로로포

름 및 에틸아세테이트에 용해되고, 헥산 및 에틸에테르에 불용 -박막

크로마토그래피(TLC) : 전개용매를 메탄올:H2O=8:2로 하여 ODS

(Octadesylsilane) TLC상에서 Rf = 0.41 -적외선 흡수대 (KBr) :

1710, 1643, 1550 cm-1 -자외선 흡수대 (MeOH) : 217, 280 nm에

서 최대흡광치

특허등록번호 : 10-774308(‘07.11.01),

PCT/KR2007/006036 (‘07.11.27) 6개국 출원,

10-774309(‘07.11.01),

PCT/KR2007/006037 (‘07.11.27) 6개국 출원

발명자 : 박진순, 염기대, 이광수, 강석구, 진재율, 박우선

헬리컬터빈발전시스템POWER GENERATION SYSTEM USING HELICALTURBINE

특허등록번호 : 10-803093(‘08.02.04),

남아공화국 2008/03046(‘09.02.25),

PCT/KR/2006/004003(‘06.10.04) 4개국 출원

발명자 : 김상진, 강성균, 황영옥, 우정희, 조장천, 강지현, 권개경

광학선택적에폭사이드가수분해효소및이를이용한광학순도에폭사이드의제조방법ENANTIOSELECTIVE EPOXIDE HYDLROLASE ANDMETHOD FOR PREPARING AN ENANTIOPUREEPOXIDE USING THE SAME

특허등록번호 : 10-896268 (‘09.04.28)

발명자 : 이희승, 신희재, 신종헌, 오기봉, 박흥식

브로모페놀계화합물, 그제조방법및이를포함하는항균조성물BROMOPHENOLS COMPOUND, METHOD OF THESAME AND ANTIBACTERIAL COMPOSITIONCOMPRISING THE SAME

특허등록번호 : 10-904982 (‘09.06.22)

발명자 : 신희재, 이희승, 정성윤

방선균분획물을유효성분으로함유하는항암용약학조성물PHARMACEUTICAL COMPOSITIONS FORANTICANCER PROPERTY COMPRISINGACTINOMYCES FRACTION AS ACTIVEINGREDIENTS

Patents Patents

● 헬리컬 터빈에 의해 회전력이 발생되므로 조력댐이 필요한 종래 구

조에 비하여 건설비용이 절감됨과 동시에 환경오염이 방지되는 효과

가 있음

● 전기를 생성하기 위해 동기발전기가 사용되어 설비비가 절감되며,

유체커플링이 장착되어 동기발전기의 과부하를 방지하는 구조로 형

성되어 기능이 향상되는 효과가 있음

● 또한, 헬리컬 터빈에 보조 지지부재와 보조 블레이드를 추가하여 회

전효율을 향상시키는 효과가 있음

● 신규 브로모페놀계 화합물 및 이를 함유하는 항균 조성물은 종래에

보고되지 아니한 신규한 화합물로, 우수한 항균 활성을 가지므로, 기

존에 보고된 항생제를 대체할 수 있어 기존 항생제에 대한 내성을

가진 세균에 의한 질병의 치료 또는 예방에 널리 사용될 수 있음

● 신규한 브로모페놀계 화합물을 제조하는 방법은 간단하여, 기존의

소량의 화합물만을 수득하던 홍조류로부터의 추출방법과 달리 신규

한 화합물을 경제적으로 대량 생산할 수 있는 방법이므로 산업적 효

과가 매우 크다고 볼 수 있음

32 | KORDI S&T No. 1 January 2010 | 33

4 : 동기발전기

5 : 유체커플링

6 : 증속기

20 : 프레임

30 : 헬리컬 터빈

유체의 일방향 또는 다방향 흐름에 대하여 연속적인 회전력을 발생시

킬 수 있도록 프레임에 회전가능하게 설치되는 헬리컬 터빈; 상기 헬

리컬 터빈의 회전을 발전에 필요한 회전속도로 증가시키는 증속기; 상

기 유체의 흐름이 일시적으로 빨라져 상기 증속기를 거친 회전속도가

필요이상으로 속도로 회전되는 것을 방지하는 유체커플링; 및 상기

유체 커플링의 회전속도를 전달받아 전기를 발생시키는 동기발전기;

를 포함하는 것을 특징으로 하는 헬리컬 터빈 발전시스템

에리스로박터 리토랄리스(Erythrobacter Iitoralis) 균주로부터 분리 및 정

제되고, SEQ ID NO : 13의 아미노산 서열을 갖는 광학선택적 에폭사이드

가수분해효소 단백질

아세트산(acetic acid, AcOH)과 디클로로메탄(CH2Cl2) 하에서 2,2'-

디하이드록시디페닐메탄(2,2'-dihydroxydiphenylmethane)과 브롬

(Br2)을 반응시켜, 화학식 1의 구조를 갖는 브로모페놀계 화합물을 제

조하는 단계를 포함하는 것을 특징으로 하는 브로모페놀계 화합물의

제조방법

(화학식 1)상기 식 중, 은 H 또는 Br일 수 있다

● 에리스로박터(Erythrobacter) 종, 스핑고피식(Sphingophyxis) 종,

노보스핑고비움(Novosphingo bium) 종 및 로도박터레일즈

(Rhodobacterales) 속 균주로부터 분리 및 정제된 광학선택적

가수분해효소 활성을 가진 단백질은 약학산업에서 약리활성이 있는

광학순도 에폭사이드를 고효율로 생합성하는데 유용하게 사용될

수 있음

● 방선균(actinomycetes) 스트렙토마이세스 속(Streptomyces sp.)에

속하는 균주의 배양물로부터 분리된 분획물을 유효성분으로 함유하는

항암용 약학 조성물 및 이것의 추출방법을 제공함

● 방선균 스트렙토마이세스 속 균주와 이것의 배양물 및 배양여과액으

로부터 얻어지는 항암물질을 이용하여 인체의 백혈병과 같은 암세

포에 대한 항암활성을 가지는 항암제 조성물을 제공함

MDA-MB-231(유방암) 7.5 0.8

HCT 15(결장암) 7.8 0.9

PC-3(전립선암) 3.2 0.7

NCI-H23(폐암) 3.5 0.5

ACHN(안암) 4.7 0.6

LOX-IMVI(피부암) 7.4 0.5

K-562(백혈병) 8.6 0.3

암세포주방선균유래항암물질 Adriamycin

IC50 (㎍/㎖) IC50 (㎍/㎖)

방선균(actinomycetes) 스트렙토마이세스 속(Streptomyces sp.)에

속하는 균주의 배양물로부터 분리된 분획물을 유효성분으로 함유하며,

아래의 성질을 보유하는 것을 특징으로 하는 방선균 분획물을 유효성

분으로 함유하는 항암용 약학 조성물. -분자식 : C8H9NO2 -분자량 :

151 -성상 : 무색의 무정형 물질로 실온에서 안정 -용해도 : 메탄올,

에탄올, 아세토나이트릴, 프로판올, 아세톤, 다이클로로메탄, 클로로포

름 및 에틸아세테이트에 용해되고, 헥산 및 에틸에테르에 불용 -박막

크로마토그래피(TLC) : 전개용매를 메탄올:H2O=8:2로 하여 ODS

(Octadesylsilane) TLC상에서 Rf = 0.41 -적외선 흡수대 (KBr) :

1710, 1643, 1550 cm-1 -자외선 흡수대 (MeOH) : 217, 280 nm에

서 최대흡광치

특허등록번호 : 10-774308(‘07.11.01),

PCT/KR2007/006036 (‘07.11.27) 6개국 출원,

10-774309(‘07.11.01),

PCT/KR2007/006037 (‘07.11.27) 6개국 출원

발명자 : 박진순, 염기대, 이광수, 강석구, 진재율, 박우선

헬리컬터빈발전시스템POWER GENERATION SYSTEM USING HELICALTURBINE

특허등록번호 : 10-803093(‘08.02.04),

남아공화국 2008/03046(‘09.02.25),

PCT/KR/2006/004003(‘06.10.04) 4개국 출원

발명자 : 김상진, 강성균, 황영옥, 우정희, 조장천, 강지현, 권개경

광학선택적에폭사이드가수분해효소및이를이용한광학순도에폭사이드의제조방법ENANTIOSELECTIVE EPOXIDE HYDLROLASE ANDMETHOD FOR PREPARING AN ENANTIOPUREEPOXIDE USING THE SAME

특허등록번호 : 10-896268 (‘09.04.28)

발명자 : 이희승, 신희재, 신종헌, 오기봉, 박흥식

브로모페놀계화합물, 그제조방법및이를포함하는항균조성물BROMOPHENOLS COMPOUND, METHOD OF THESAME AND ANTIBACTERIAL COMPOSITIONCOMPRISING THE SAME

특허등록번호 : 10-904982 (‘09.06.22)

발명자 : 신희재, 이희승, 정성윤

방선균분획물을유효성분으로함유하는항암용약학조성물PH