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Ihda Uswatun Shalihah Shohibuddin and Wan Wardatul Amani Wan Salim* Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University Malaysia, Gombak 50728, Kuala Lumpur, Malaysia.
Email : [email protected] and [email protected]*
ABSTRACT
ACKNOWLEDGEMENT: The research work is funded by NanoSkunkworkX Sdn. Bhd through a Sponsored Research Grant Awarded to the Amani Research Group.
REFERENCES:
1. Soum et al., (2019). Programmable paper-based microfluidic devices for biomarker detection. Micromachines. 10 (8): 516. https://doi.org/10.3390/mi10080516
2. Park et al., (2017). An autonomous lab on a chip for space flight calibration of gravity-induced transcellular calcium polarization in single-cell fern spores.
Lab on a Chip. https://doi.org/10.1039/C6LC01370H
Microbotics CaLSens: NANOTECHNOLOGY-BASED CALCIUM SENSOR
FOR EARLY DETECTION OF PREGNANCY COMPLICATION
METHODOLOGY
Ca2+ level
1200 mg/day
13/2/2020
Patient A
Ca2+ level
600 mg/day
13/2/2020
Patient B
Microbotics CaLSens.
13/2/2020
Patient A
Patient C
Patient D
Patient B
1200
1500
1000
600
Hospital Information
System
Ca2+ ok
Go see
doctor
To develop a nanosensor dot
To characterize and evaluate the
developed sensor
OBJECTIVES
PROPOSED SOLUTION
PROBLEM STATEMENT
70 % 6 %
40 % Preterm delivery
Reduced bone calcium
content
Reduced fetal growth GLOBAL CALCIUM
DEFICIENCY
By developing a calcium sensor.
Microbotics CaLSens:
FINDINGS
COMMERCIALIZATION POTENTIAL
WHERE CAN WE GO NEXT?
The proposed nanotechnology-based calcium sensor provides an
initial insight for early detection of pregnancy complications by
constant monitoring of calcium ion in bodily fluids.
CONCLUSION
2018
USD 23.98 B
2026
USD 50.51 B
240 mV
scan starts
30 mV
scan starts
Δi = 0.965 mA
scan starts
1) SHIFT IN PEAK POTENTIAL, EP
2) PEAK
CURRENT,
IP
3) EP & IP
NOVELTY OF IDEA An nsD Technology
Commercialization potential
Point-of-care (POC)
Diagnostics Market Size CAGR
of 9.6 %
Future directions
with respect to
nsD Technology
A microfluidic
POC Device
-- negative control -- nsD 1 -- nsD 2 -- nsD (1 & 2)
nsD 1
nsD (1 & 2) nsD 2
0.030 0.030
0.240 0.180
0.281
0.076
1.041 0.998
0.000
0.200
0.400
0.600
0.800
1.000
1.200
SPCE rGO:PSS/SPCE PEDOT:PSS/SPCE rGO:PSS-PEDOT:PSS/SPCE
Shift in peak potential, Epa (V) Peak Current, Ip (mA)Ep
negative nsD 1 nsD 2 nsD (1& 2)
control
Hig
her
i p
Smaller shift in Ep
ip = 0.998 mA ip = 1.041 mA
Ep = 30 mV
Ep = 240 mV
ip = 0.076 mA
Ep = 180 mV
nsD 1
ARG Group
Member
Real-time
monitoring
Portable Calcium
sensing Miniaturization
nsD 2
Body fluid flow Sensor readout
We aim for a next-generation point-of-care (POC) device that is sensitive with high stimulus-responsive properties to monitor calcium levels during
pregnancy. The POC device consists of nanosensor dot (nSD) transduction technology that are 100 times more sensitive than a conventional
transducer used in a normal glucose meter. We have successfully characterized the nSD via electrochemical cyclic voltammetry (CV)
measurements where the CV graphs showed the nSD to have fast electron transfer kinetics at the electrode-electrolyte interface, illustrated by the
small shift in peak potential (Ep) and high peak current (Ip). Since the size of global blood testing market was estimated at USD 23.98 billion in 2018
and is expected to be doubled in 2026, the proposed technology has the potential to be commercialized. The nSD technology is funded by
NanoSkunkworkX Sdn Bhd through a Sponsored Research Grant awarded to the Amani Research Group, International Islamic University Malaysia.
Sample
inlet
Microfluidic
channel
Sample
outlet
