Embed Size (px)
Citation preview
7/23/2019 pembuata pereaksi
http://slidepdf.com/reader/full/pembuata-pereaksi 1/13
LAPORAN PRAKTIKUM
PEMBUATAN PEREAKSI
NAMA : DWI NICHE
NIM : H311 12 264
HARI/TANGGAL PERCOBAAN : RABU/ 20 FEBRUARI 2014
KELOMPOK : III (TIGA)
ASISTEN : SAKINAH NUR FADILAH
LABORATORIUM BIOKIMIA
JURUSAN KIMIA
FAKULTAS MATEMATIKA DAN ILMU PENGETAHUAN ALAM
UNIVERSITAS HASANUDDIN
MAKASSAR
2014
7/23/2019 pembuata pereaksi
http://slidepdf.com/reader/full/pembuata-pereaksi 2/13
BAB I
PENDAHULUAN
1.1 Latar Belaa!"
C!" #$%"& '!$"% *"+" +$,"! '"&% !"%"- C!" '&"+%!" .%$& $""
"!#$" $&"+% "+"* "+$#"# '" "+"* "+$#"# *$"!" &"+% +"* '"
$*"#" "#" " - P"'" ""' !$3 S$$%* M"+$& F%+5 " !.
T&$.&"+#.+ *$,$%"+!" "&7" 8!" $$"!+ '$" %."*%."* *$*$#!
$"" 9"# 7"" *+"%" #*"% #& (#*"% !"."#)- P"'" ""' !$;
%*7" P$+" <" H"" *$&"+%!" "+"* "+$#"# $!"# '" 8!" *$%"%
'+#%"+- S$,"! 1=10 !$""!" "+"* "+$#"# '&"+%!" '" cairan piroligneous
" '$.%$& '" '+#%"+ !"- C"" '$"!+!" '$" !"%+* &'.!+'"
*$&"+%!" !"%+* "+$#"# " !$*'" '"+"*!" '$" "+"* +%5"#
*$&"+%!" "+"* "+$#"#-
A+"* "+$#"# *$"!" +"%"& +"# "+"* !".!+%"# "% +$'$&""- A+"*
"+$#"# "+"* $#"."# "#" "+"* 8!" "'"%"& +$"7" !*" "+"* ."! "
'!$"% +$"" $*$ "+" "+"* '" ".*" '"%"* *"!""- A+"* 8!" *$*%!
*+ $*+ C2H4O2- R*+ +$!"% '#%+ '"%"* $#! CH3COOH
CH3COOH "#" CH3CO2H-
U**" "+"* "*. $+ .#$ #$' '" 20 ,$+- O%$& !"$"
+5"# !&"+ " '*%! "+"* "*. $$"" ,$+ "+"* "*. #$+$# +$
',"'!" $$"!+ #! !$$%" %"."#.*- A'" $$"!+ "+"* "*. "
$# '!$#"& "'"%"& "%" %+ '" "+"* "+"#"#-
O%$& !"$" # '%"!!"%"& $8."" $*"#" $$"!+ '" #"
7/23/2019 pembuata pereaksi
http://slidepdf.com/reader/full/pembuata-pereaksi 3/13
"+"* !".!+%"# "# "+"* "+$#"# '" "'" %" %"#" $%+. +$#" $$"!+ '"
"+"** "*. "# "%" %+ '" "+"* "+"#"#- U#! $8."" !"% "#
*$*"# $$"!+ %"#" "+"* "+$#"# $%+. "%" %+ '" "+"* "+"#"#-
1.2 Ma#$% %a! T$&$a! Per'()aa!
1.1.1Ma#$% Per'()aa!
M"!+' '" $8."" "'"%"& #! *$$#"& '" *$*$%"," 8""
$*"#" $$"!+ "#" $"$ %"#" "+"* "+$#"# N$%+. A "+"* "+"#"# %+
'" %$+-
1.1.2T$&$a! Per'()aaa!T," '" $8."" "'"%"& #! *$*"# $$"!+ "#" $"$ %"#"
"+"* "+$#"# N$%+. A "+"* "+"#"# %+ '" %$+-
1.* Pr+!#+, Per'()aa!
P+ '" $8."" "'"%"& $*"#" $$"!+ %"#" "+"* "+$#"#
N$%+. A "+"* "+"#"# %+ '" %$+-
BAB II
TINJAUAN PUSTAKA
A+"* "+$#"# *$"!" +"%"& +"# .'! '+# " ""! '#&!" '
I'.$+"- A+"* "+$#"# '""# '"# '" ++#"# " *$"' $#".% "
'""# '$.%$& '" $"" *"8"* "&" +$$# "&"&" !%# ""+ %
!. '" " !$%""- T$+$'"" " !$%"" '"%"* ,*%"& $+" ' I'.$+" "#
7/23/2019 pembuata pereaksi
http://slidepdf.com/reader/full/pembuata-pereaksi 4/13
%$& '" =00 ,#" %#$ $ #"& *$"!" .#$+ " $%* '*"5""#!"
+$8"" *"!+*"% (I" N!" '" N H'""# 2001)-
G%+ *$"!" "+"* "*. " "% +$'$&"" '" '""# $'+.+"+
*$*$#! +"# ". %+ H2 NCH9CO2 " '""# $#'"! +$"" %"
#$&"'" !"#. %."* #"++- G%+ '.%.!" !$"'" %" '$#"# %" +$*
'" %" $"#5 K"$" *$*" "+"" $%$!#. $"+ '"%"* "#.* N '"
"+"" $%$!#. '"%"* "#.* O +$"" !$%$&" $%$!#. (E'' S',"" '!!
2002)- G%+ '""# *$"%"* $"!+ '$""+ .!+'"#5 .%$& %+ .!+'"+$ "#
$9* " #$'""# '"%"* ,"" &"# '" ,"%- D"%"* $"!+ %+ "!"
'"& *$,"' "+"* %.!+%"# '" "**."- A+"* %.!+%"# " #$$#! '""#
'"!" %$& %",# *$,"' 5.*"%'$&'" '" !".'.!+'" (A" P '" T#
F-M-S 2012)-
CH2(NH2)COOH > ? O2 CHO @ COOH > NH3
A+"* %.!+%"# '""# ," '"& *$,"' "+"* *"%"# " *$,"%"
*$#".%+*$ *$%"% +!%+ "+"* +#"#- D+"* # %+ '""# '"& %"
*$,"' +$ '$" 5.*%#$#"&'.5.%"#- D"%"* $"!+ 5.*%#$#"&'.5.%"#
$5+ +$"" '.. + 5.*% !$"'" %+ (A" P '" T# F-M-S 2012)-
G%+ '""# $5+ '"%"* .+$+ $"7" "8 *+"%" ""%" "+"*
$9."#$ '" '$"#" #$*"+! '"%"* *"!"" *"!" %+ "!" $"
'$" 9"#9"# #$+$# +$&" #$$#! "+"* &"# " #'"! $+5"# "8
(A" P '" T# F-M-S 2012)-
D"%"* #& %+ '""# '$#! '" +$ '"%"* ,*%"& " 8!
!"$" # %+ "'"%"& "+"* "*. .$++$+"%- S$ '$#! '" "+"* 3
5.+5.%+$"# " *$"!" +"%"& +# &"+% "#"" '"%"* .+$+ %!.%++- D$"
7/23/2019 pembuata pereaksi
http://slidepdf.com/reader/full/pembuata-pereaksi 5/13
'$*!" '""# '%&"# "&7" "'" &" "#"" %!.%++ '$" .+#$++
%+ (A" P '" T# F-M-S 2012)-
L$+ '""# '"& *$,"' "+"* !$#. *$%"% $"!+ #"+*"+ .!+'"#5-
K$*'" "+"* !$#. *$%"% $$"" #"&" $"!+ '"& *$,"' "+$#% K.A-
S"%"& +"# +$"7" " #$$#! '"%"* #"&" $"!+ #$+$# "%"& *$#% %#"*%
K.A (HMG C.A) " ," *$"!" +"%"& +"# 9"# "#"" '"%"* .+#$++
!.%$+#.% (A" P '" T# F-M-S 2012)-
COOH
CH2
CH3 C OH
CH2
C S K.A
O
N"#* !"."# "#" '+$# ," "#* &'.$ !"."#
*$"!" +$"7" !*" '$" *+ N"HCO3- B$" "'"#" #& "
!+#"% "* #$!"'" $" +$! #& &"%+ +$'!# #$"+" "%!"%
*$$" "#* !"."#- S$"7" *$"!" !.*.$ '" *$"% "#.
'"%"* $$"" +*$ *$"% %"- B$#! *$"% "%"*" '!$"% +$""
"&!.%#- T" +$"7" "+"" ," '.'!+- S$*$,"! $"""
+$*"! *$%"+ "#* !"."# *$*" $$"" "*" '"" "#""
%" "! +.'" +.'" !$ +.'" *"+"! '" +.'" .!"."# (I'"& A 200=)-
G""* R.&$%%$ *$"!" +$! "#" #" #'"! $7"" &" #&
!$" '$" +#!# !+#"% .#..*! " *$"!" ""* " $"+" "+
'" '- S$8"" !*" ""* R.8&$%%$ *$"!" N"#* S.' T"#"#
7/23/2019 pembuata pereaksi
http://slidepdf.com/reader/full/pembuata-pereaksi 6/13
(KN" (C4H4O6)4H2O) " %"# '"%"* " '" +$'!# %"# '"%"* "%8.&.% '$"
##! %$%$& . C +$+5! "5# 1=- G""* *$,!!" $,"%" $*"+"
"'"- K+#"% '" ""* .&$%%$ +""# *'"& #! '+#$++ '" "+"" +$
'"!" +$"" "&" $*"#" "%"# " %+#! +$&" '$" +5"#"
" "!" *$*$!" #$"" +""# '!$" #$!"" *$!"! " '+$# +$"
+5"# $9.$%$!#! (I'"& A 200=)-
S#!# ""* R.8&$%%$
G""* R.8&$%%$ *$"!" "'"#" " !+#"% '$" $5$! $9.$%$!#!
" $+" "# "!" *$'!+!" *"#" %+#! "'" $*!""" +""#
'!$" '$5.*"+ *$!"! +$$# #$!"" "#" #$!!"- S5"# +""# $*"5""#
#! $*"#" "%"# " *$*$%!" +$+#5#"+ "!+#! "#" "+."%- S$$#
&"%" *"#$"% $9.$%$!#! %"" ""* R.8&$%%$ "!" *$$" ,!" $"'"
"'" *$'" %+#!- M"#$"% "!" #$'$!.*.++ "'" #$*$"#$ +$'" &"
# . C (131 F) '" *$*#&!" $%'" #! *$%"7" !$%$*""-
D$5.*"+ $9.$%$!#! +$"' '$" *$'" %+! " '$!" '"
$"' #$"%! '$" .%"#"+"- S5"# '"+" " '$#*"!" +$8""
$%$!#.!*" "&7" ""* R.8&$%%$ '""# '"!" +$"" #"'+$ $"%"#"
$%$!#.! +$$# $$"#. %#"+.8 *!.5. pick-up 5.."5 +$#" $+."#.
$%$!#.!*" ( I'"& A 200=)-
7/23/2019 pembuata pereaksi
http://slidepdf.com/reader/full/pembuata-pereaksi 7/13
BAB III
METODE PER-OBAAN
*.1 Baa! Per'()aa!
B"&""&" " '"!" "'" $8."" "'"%"& N"2CO3 "&'"#
""* R.8&$%%$ N"HCO3 N"2SO4 "!"'$+ tissue roll '" +"-
*.2 Alat Per'()aa!
7/23/2019 pembuata pereaksi
http://slidepdf.com/reader/full/pembuata-pereaksi 8/13
A%"#"%"# " '"!" '"%"* $8."" "'"%"& $%"+ !*" 20 *L
$%"+ ! 100 *L "#" $"'! +$'.! #"'! '" +!"# #"-
*.* Pr(#e%$r Per'()aa!*.*.1Pe/)$ata! lar$ta! A#a/ A#etat
D+"!" $%"+ ! 100 *L #$%$& '"&%- D#"!" +$""! 0 *L
CH3COOH 02 M !$ '"%"* $%"+ ! 100 *L !$*'" '$8$!" &" .%*$
100 *L- L"#" CH3COOH %"% '&.*.$!"- S$#$%"& '&.*.$!" %"#"
'*"+!!" !$ '"%"* .#.% $"$#-
*.*.2Pe/)$ata! lar$ta! Nel#(!
D+"!" $%"+ !*" 20 *L #$%$& '"&%- D#*" +$""!
"* N"2CO3 "&'"# "* ""* R.8&$%% 4 "* N"HCO3 40 "* N"2SO4-
D%"#!" +$*" "&" '$" "!"'$+ &" .%*$ 200 *L- L"#" $%+.
'"'! '$" *$"!" "#" $"'!- S$#$%"& +$*" "'"#" %"# %"#"
'*"+!!" !$ '"%"* .#.% $"$#-
*.*.*Pe/)$ata! lar$ta! Ala!+!
D+"!" $%"+ !*" 100 *L '" $%"+ !*" 0 *L #$%$& '"&%-
P"'"#" "%" +$8!" '*"+!!" !$ '"%"* $%"+ !*" 0 *L '$"
*$"!" +$'.! #"'!- L"#" "%" '%"#!" '$" +$'!# "!"'$+-
L"#" ''"&!" !$ '"%"* $%"+ !*" 100 *L- D#"*"&!" '$" "!"'$+
&" #"'" "#"+ $%"+ !*" 100 *L- L"#" "%" '"'! '$" *$"!"
"#" $"'!- S$#$%"& +$*" "'"#" "%" %"# %"#" '*"+!!" !$ '"%"*
.#.% $"$#-
*.*.4Pe/)$ata! lar$ta! A#a/ A#,artat
D+"!" $%"+ !*" 100 *L '" $%"+ !*" 0 *L #$%$& '"&%-
P"'"#" "+"* "+"#"# +$8!" '*"+!!" !$ '"%"* $%"+ !*" 0 *L '$"
7/23/2019 pembuata pereaksi
http://slidepdf.com/reader/full/pembuata-pereaksi 9/13
*$"!" +$'.! #"'!- P"'"#" "+"* "+"#"# '%"#!" '$" +$'!#
"!"'$+- L"#" ''"&!" !$ '"%"* $%"+ !*" 100 *L- D#"*"&!" '$"
"!"'$+ &" #"'" "#"+ $%"+ !*" 100 *L- L"#" "+"* "+""# '"'! '$"
*$"!" "#" $"'!- L"#" "+"* "+"#"# '#"*"&!" '$" #"
#$#$+ HC% 4 N "" %"# +$*"- S$#$%"& +$*" "'"#" "+"* "+"#"# %"# %"#"
'*"+!!" !$ '"%"* .#.% $"$#-
BAB IV
HASIL DAN PEMBAHASAN
4.1 Ha#+l Per+t$!"a! Perea#+
4.1.1 Per+t$!"a! Pe/)$ata! A#a/ A#etat 01 M #e)a!a 100 /L
V 1 M 1=V 2 M 2
1M1
2
M2
100 *L 01 M
2
02 M
2 0 *L
7/23/2019 pembuata pereaksi
http://slidepdf.com/reader/full/pembuata-pereaksi 10/13
4.1.2 Per+t$!"a! Pe/)$ata! Perea#+ Gl+#+! 001 M #e)a!a 100 /L
L - M - M
01 L - 001 *.%/L - 0 /*.%
000 "*
4.1.* Per+t$!"a! Perea#+ Ala!+! 001 M 100 /L
)(
1000
mLV x
Mr
Massa M =
4.1.4 Per+t$!"a! Perea#+ A#a/ A#,artat 001 M 100 /L
)(
1000
mLV x
Mr
Massa M =
4.2 Pe/)aa#a!
P$*"#" %"#" "+"* "+$#"# '" %"#" $%+. "'" **" +"*" '"%"*
+ $$,""" $!# + $*"#""- A%"#"%"# " '"!"
'+#$%!" #$%$& '"&% +$$%* '"!"- S$#$%"& # '#"!" +$""! 0
*L CH3COOH 0-2 M !$ '"%"* $%"+ ! 100 *L- L"#" CH3COOH '%"#!"
'$" 0 *L "!"'$+- L"#" CH3COOH %"% '&.*.$!"- S$#$%"&
'&.*.$!" %"#" '*"+!!" !$ '"%"* .#.% $"$# '" !$*'" '$
%"$%- P"'" $*"#" %"#" $%+. #$%$& '"&% '#*" *"+*"+
"&" "'"#" " "!" '8"*!" '$" *$"!" $"8" .&* "#
+$""! "* N"2CO3 "&'"# "* ""* R.8&$%% 4 "* N"HCO3 40 "*
N"2SO4- K$*'" '"!" !$#"+ #*" #! *$*'"&!" *"+*"+
"'"#" !$ '"%"* $%"+ ! 200 *L- +$*" "&" " #$%"& '#*"
'#"*"&!" '$" "!"'$+ &" #"'" "#"+ $%"+ !*" 200 *L- L"#" $%+.
'"'! '$" *$"!" "#" $"'! &" "'"#" %"# '"%"* "-
S$#$%"& +$*" "'"#" %"# %"#" '*"+!!" !$ '"%"* .#.% $"$# '" '$
%"$%-
7/23/2019 pembuata pereaksi
http://slidepdf.com/reader/full/pembuata-pereaksi 11/13
P$*"#" %"#" "%" %+ '" "+"* "+"#"# "'" **" +"*"
'"%"* + $$,""" $!# + $*"#""- A%"#"%"# "
'"!" '+#$%!" #$%$& '"&% +$$%* '"!"- S$#$%"& # *"+
*"+ "'"#" "%" %+ '" "+"* "+"#"# '"*% +$8!" '$"
*$"!" +$'.! #"'! '" '%"#!" '$" "!"'$+ '"%"* $%"+ !*" 0
*L %"% ' "'! '$" *$"!" "#" $"'! &" "%" %+ '"
"+"* "+"#"# *"+*"+ %"# !$*'" '*"+!!" !$ '"%"* $%"+ !*" 100
*L- +$%",#" "!"'$+ ' #" !$*"% !$'"%"* $%"+ !*" #$*"# *$%"#!"
"%" %+ '" "+"* "+"#"# *"+*"+ +$""! 100 *L-- S$%",#"
'&.*.$!" '$" $"'!" *$"!" "" $"'! '" '*"+!!"
!$'"%"* .#.% $"$ !$*'" '$ %"$%- U#! $*"#" %"#" "+"* "+"#"#
'$ $%"!" !&++ '"!& .+$' +$" "+"* "+"#"# +%# %"# '"%"* "
+$&" $% '#"*"&!" "+"*- A+"* " '"!" "'"%"& HC% 4 N-
L"#" "%" %+ '" "+"* "+"#"# '"# '"%"* $#! %"#" #!
'"!" +$"" +"*$% ,$+ "+"* "*. " "!" , "#" ''$#5!"+ *$%"%
$$"" $$"!+ +$$# $"$# &' "#* #.+'" "**.*
&'.!+'" $# H.!+8.%$ '" *%%.-
7/23/2019 pembuata pereaksi
http://slidepdf.com/reader/full/pembuata-pereaksi 12/13
BAB V
KESIMPULAN DAN SARAN
.1 Ke#+/,$la!
P$*"#" %"#" "+"* "+$#"# '%"!!" '$" *$%"#!" %"#"
CH3COOH 01 M +$""! 0 *L '$" "!"'$+ 0 *L " !$*'"
'&.*.$!"-
P$*"#" %"#" N$%+. A '%"!!" '$" *$%"#!" +$*" "&"
8"*" "'"#" "# +$""! "* N"2CO3 "&'"# "* ""* R.8&$%%
4 "* N"HCO3 '" 40 "* N"2SO4 !$ '"%"* $%"+ !*" !$*'" '%"#!"
&" .%*$ 200 *L %"% '&.*.$!"-
P$*"#" %"#" "%" '" %+ '%"!!" '$" *$%"#!" "'"#""
*"+*"+ !$ '"%"* 100 *L "!"'$+ !$*'" '$ 5+ $%"!" '$"
$"'!" #! *$*$8$"# 9"# #$%"# %"# '"%"* $%"#--
7/23/2019 pembuata pereaksi
http://slidepdf.com/reader/full/pembuata-pereaksi 13/13
P$*"#" %"#" "+"* "+"#"# '%"!!" '$" *$%"#!" "'"#""
*"+*"+ !$ '"%"* 100 *L "!"'$+ !$*'" '"'! '" '#"*"&!"
$$"" #$#$+ HC% 4 N #! *$*$8$"# .+$+ $%"#"-
.2 Sara!
S$"!" +$$%* *$%"!!" $8."" +$*" "%"# '" "&" " "!"
'"!" '"%"* $8."" +'"& '+"!" +$&" "!#!" #'"! !$"
'"%"* *$8" "%"# '" "&" " '"!"-
