P.parasit MSTE------------EVVA-------VEEQEIPDVIERLPKPDKAEHEAKISALDGAIK 41G.sulph MANTT------------------SQGPTTQSQAMAVEQKLLTPE--ERQEEIRKLEQKKQ 40ScBFR1 --------------------------------MSSQQHKFKRPDVSVRDKKLDTLNVQLK 28Endotryp -----------------------------------MPGALEKPKLSEYSAKISGLVEQRR 25Paratryp MSRSASAEAVATEPATTVVAEAPRRKPGQKPGWMSIPGGPKEPDRQAFSAKIREFGDRKR 60Bodo ----------------MAAVPAAENRPRTRPGWMNIPGGPKEPNFQDFRNKMQTLNTQKT 44Blechomonas ---------------MSAASPKEEHQRKRLPPWMSMPGAPPKPDFSAFGGKMAQLSAEKK 45T.rangeli MPGK---EIMSG---TADVAVATPAPSRRMPPWLLTPGGPKEPNTAEFRAKMAALAEEKR 54T.cruzi ----------MT---STEAAAVVAAPSKRMPPWLSMPGGPKEPNSAAFRAKMSALAEEKR 47T.grayi -----------------------------------MPGGPPEPNAAAFRSKMSRLAEEKR 25T.theileri --------MSAT---PPPAAAAAPTAKKQMPGWMSIPGGPPEPNGAAFRAKMNRLVEERR 49T.vivax -----------M---TSKVQTGAVEARRPPPRWMTGPDALPKPNVAEFRAKIAQLAKEKN 46T.brucei MSK-------TE---TAPEAAPAPTERRPQPRWMTAPGAPPRPDVMEHRTKMKALSQEKG 50T.congolense MGGD------AT---EVVAPAAGPAVRRGLPKWMTGPGAMPKPDFRAFKAKMAELAEKKR 51

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P.parasit KLQARTNVIRAEMDALKTNRGGYGGQIQ----------------------EAKAKF---- 75G.sulph ELIQQIVVVQQPSKDELETAVAAQRAIIESALSQKEQKRLLQRQKYQKMEETKPEFLAAK 100ScBFR1 KIDTEIGLIRKQIDQHQV-----------NDT---------------------------- 49Endotryp ALLDELKQLQSTVQNDPE-----------RQK---------------------------- 46Paratryp ALFDQLKEVQEKLHANGG-----------NEA---------------------------- 81Bodo KLFDNLKELQGRAGPRDD---------TEREA---------------------------- 67Blechomonas KLFEEIKRLQNSIRGDTS-----------NEA---------------------------- 66T.rangeli TLLTEVRQLRASLGPREG-----------REA---------------------------- 75T.cruzi ALLNEVKQLRASLGPREG-----------REE---------------------------- 68T.grayi ALFTEVKQLRASLGPREG-----------REE---------------------------- 46T.theileri ALLAEVKTLRASLGPREG-----------QEA---------------------------- 70T.vivax TLFDKIKELRATLEPRDE------------------------------------------ 64T.brucei ALIAQIKELRASLGPKSE------------------------------------------ 68T.congolense SLIAQVKGLRASIGPKGD------------------------------------------ 69

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P.parasit -------AALRAEKDNLFQ-----------------QRNQITARLRQTRDEKDSTIKQQR 111G.sulph AAYEEQVTLLRKLREERKQLMGKIQETRKLMNDAQDQ---------NKSNNISTGNAALD 151ScBFR1 ---------TQQERKKLQDKNKEIIKI---QADLKTRRSNIHDSIKQLDAQIKRKNNQIE 97Endotryp ---------CIAERNAFGEELNEIDACRKVQRELRAVQNAKIAKLRKSRTEVTEKLRSVQ 97Paratryp ---------IAVERQALRDRLGELEKERRGQRDVLTAKNEEIAALRKQRREIQDKQKELS 132Bodo ---------VMGERQELRRRMNEIDANRKKERDARSTKNEEISRIRRQRSDIEGKLKELS 118Blechomonas ---------LDEERKALRQRMGEIEAQRSAMRDLRVGKSEEISKFRKKRQETADKLRVLQ 117T.rangeli ---------TDNELAELRQRMSDIDARRKMEQEMRFKKNEEIQRVQKLHDERQSRLRELS 126T.cruzi ---------SGKELDGLRQRMGEIDNQRKVEQEMRFKKNEEIQRIQKLHDERVSRLRELS 119T.grayi ---------QENELGELRQRMGEIDTQRKAEQEMRFKKNEEIQKIQKVHEERLSKLRELS 97T.theileri ---------VEAELSELRQRMGEIDTQRKAEQEMRFKKNEEIQKIQKIHDERLNKLHELS 121T.vivax ---------NDKEREELRERIKELDNKKKLENDLRKKKSDEIQKVKKVQEEQLKKLRELT 115T.brucei ---------PDKERDEIRQRLKELDDKRKAEQEMRSKKSEEIIEVRKKHDEYVKKLRSLT 119T.congolense ---------NGKERDELFQRIKSIEEMRKAEQEMRSKKSSELNEAKKKHNENARKLRELT 120

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P.parasit -SVRANLKYGSVAEFDAAIAELKHKQETSSMSLNEEKRVIKEIEQLQAQKQQ-VSGFSDD 169G.sulph EK---LKNFKNLEQLDKHISSLERRQATESLSLTEEKKLVSEISLLKNKGRAFLENMQKL 208ScBFR1 EKLGKKAKFSSTAEAKQRINEIEESIASGDLSLVQEKLLVKEMQSLNKLIKD-LVNIEPI 156Endotryp AE---VGGFTTLKEIDEAIDHMMRKMETSGGGLVAERRNQQHLQKLEEAKMH-LQKLQPL 153Paratryp TE---LGGFKTVQEIDEAIDYIMLKMETGGGGLSSEKKTAKRLHQLGEAKNL-LLQLQPL 188Bodo NE---LGAFRELSDIEMAIDHIMVRMETSGGGLASEKKAIKRLSQLEEAKSL-LLQLQPL 174Blechomonas AE---LGGFTDIAEIDTAIEFVMRKMETSSGGLAAEKRTIKRLQQLEEVKSL-LLQLQPL 173T.rangeli ED---LGGFTTLKEIDNAIALITRKMETSGGGLAAEKRTVRQLSKLEEAKRY-LVELQPL 182T.cruzi DD---LGGFTTIKEIDDAIAFVTRRMETSGGGLGAEKRAVRQLSKLEEAKRY-LVELQPL 175T.grayi DE---LGGFKSLKEIDGAIDYLTRKMETSSGGLAAEKRAVRQLNKLEEAKRY-LLELQPI 153T.theileri DG---LGGFKTLKEIDEAIAYLTRKMETSGGGLAAEKRTVRQLSKLEEAKRF-LLEMQPL 177T.vivax TE---LSGFKSVEEIDEAIAYMTKKMETSGGGLAAEKRMLRQLSQLEDAKRY-LQELQPV 171T.brucei DD---LGGFKSVEEFDEAIEYMTKKMETSGGGLAAEKRIMRQIGQLEDAKRY-LQELQPV 175T.congolense EE---LAGFKSVEEIDRAIVYMTRKMESSGGGLAAEKRMLQQLAQLEGAKRY-LQELQPL 176

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P.parasit QGVVEKQNE-SIKEIRALQT---KKNEEIDAIQEKLNEQKQALDELYRL-----NEEENK 220G.sulph EAERKSSQEERKKELEKLFEEKKSLETKINETSKELERLKQSKDDIRTK-----QEISIA 263ScBFR1 RKSV---DA-DKAKINQLKEELNGLNPKD--VSNQFEENQQKLNDIHSKTQGVYDKRQTL 210Endotryp TEAI---KE-ITEEEVILQQEYLAICEKIGILNGEYEEKLKQKNAKH-------KEAQED 202Paratryp DEAL---SE-FEEREFGLQQEYREIHERIGVINKEYEVQNDVRRK-----------ISDN 233Bodo QEAI---TD-ADDREASLQQEYREIHERIGALNKDYDEQYTVKQSKD-------KEAQKT 223Blechomonas TEAI---QE-AGDREVMLQQEHREIHERIGALNKKYEEELHTKQATE-------KNLHTT 222T.rangeli TEAI---TE-AKHREAMLQREWQEINDRIRSLWSEYNEQRATKQQKE-------QEIRIT 231T.cruzi TEAI---TE-AKHREAMLQREWQEINERIRNLRAEYNEQRATKQQKE-------QEIRSS 224T.grayi TEAI---TE-AKHREAMLQREWQEINERIRNLGTEYNEQRSMKQQRE-------QEMHST 202T.theileri TEAI---TE-AKHREALLQREWQEINERVRNLKSEYNEQRMTKQQKE-------QELRKT 226T.vivax NEAI---AE-AKHREALLQKEFDEINERIRGLTTACNEQRSTKMEKD-------QLVRGK 220T.brucei SEAI---AE-AKHCEATLQREIQEINERIRGLNEEYKGQRSTKMEKD-------DKMRST 224T.congolense SEAV---DE-AKHHEATLLRELQEINERIRDLNSKYKDQRSVKMQKD-------EEIRSA 225

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P.parasit KDKFPALAKERKEIKEQLDEKFTAIKTLRKEFKEANDKYYNNIRLVRKKKELERQKEEEA 280G.sulph KISEELSEVNLDDIQKRIDAANEEIRRLRQDFNQKLNKWYNYKKRSSE-------LDRLN 316ScBFR1 FNKRAALYKKRDELYSQIRQI-------RADFDNEFKSFRAKLDKER------LKREEEQ 257Endotryp GAHRAEVYKKCDALRVRIAEISQTVESLRAERDRLSSEWHAWSREARTKYYAQLEQRREQ 262Paratryp IVDKKPLFERRDLIRKELDAVSVEMDTLRAAHRQAEEAWDKWRVEAQAKYAAKIKAEREE 293Bodo SVDRTVLIKERDDLRQKITKLNEEMTKLREGFNTEKESWEAWREEAIKKYGEKMEAERKE 283Blechomonas FQQRTSVYKQCDELREKINKLNEEMDTYRASHNDAVQKWETWCAEAREKYTAKMETERQE 282T.rangeli GVNRSEVYKKCEEISAKINKLSEEMNRMREEHNKAMEAWNAWREEARAKYLAKMEAERKE 291T.cruzi GVNRAEIYKKCEEINGKITKLSGEMDRLREEHNKAMEAWNTWREEARAKYIAKMEEERKE 284T.grayi GANRAEVYKKCDAISAKINKLNEEMNTLREEHNKALEAWNAWREEARTKYAAKVEAERKE 262T.theileri GVNRQEVYKKCDEISAKIKSLSDEMNTLREEHNKAVEAWNAWREEARAKYAAKVEAERKE 286T.vivax GASRQEVYAKCSELHAKITGIVEKMNTLREEHSKAMEAWNAWRAEAFAKHQAKMEEIRKE 280T.brucei GANRQEVFKKCDEISAKITSITKEMNALSEDFQKAMEVWKSWCDEARAKHMAKMEEVRKE 284T.congolense GANRQEVFKKCDEINANIAKIAQEMNSLSEAHQKAMEKWNAWCDEARAKHIAKMEEIQKD 285

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P.parasit RKAEY--E------AKL--ADYEKEMAKIHPYQDEMDLCDALVSFLEKTYAKELKEEQDE 330G.sulph RHLRFQYRQYQRDLKRVEKEKELAEYGTPDPYEEEKAMCDNLIQ-----YLQRLSKIDVD 371ScBFR1 RLSKLLEQKDV-DMGKL-QEKLTHAKI--PAFTYEIGAIENSLLVLDPTYVKPKKNILPD 313Endotryp RRKEYEEIRN---AHKI-AAKRERAAKRQNPYVTEISACATLIQ-----YLKQKKVMLEQ 313Paratryp KLRRYLERRD---ASKL-AEKRERAMKRMNPYASEIASCITLVQ-----YLRDKRMMSQR 344Bodo RERRRNEYMN---AEKI-ARKQARATKRQNPHETQIGACSTLVR-----YLRDRIVMSQR 334Blechomonas RYKRELERHT---NAKL-TEKLNRAQRRRNPYEMEISACDTLSQ-----YLIDKKHMVTR 333T.rangeli RQRRYLERKN---AAKL-EEKRARALRRQNPYEVEIEACKTLLR-----YVQDHKVMVQR 342T.cruzi RRRRYLERKN---AAKI-EAKRARALRRQNPYEAEIDACKTLLR-----YMQDQKVMVQR 335T.grayi RHLRYLERKN---AAKL-EEKRARAMRRQNPYEAEVDACSVLVR-----YLRDHKMMVQR 313T.theileri RQRRYLEYKN---AAKM-EEKRARALRRQNPYEAEADACSTLVR-----YLRDKKAMVQR 337T.vivax RERRIHEKNN---AAKL-EEKRARALRRMNPYEVEIAACDTLIQ-----YLREQKVMVKR 331T.brucei RHRRFLERKN---APKL-AEKRERALRRMNPYEVEIAACDTLLQ-----YLRDQKIMVQR 335T.congolense RQRRIMERKN---AAKL-EEKRARALRRMNPYEVELAACDTLLR-----YLGEQKIMVQR 336

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P.parasit K---------AAETTAAPLE-LDGMKPLQRKEEDFMMLGGGKKGK--KGRNGK------- 371G.sulph ENKKTKKDVQNIE--------LNGAKLIGKNASFFEAEPNTNVSKG-------------- 409ScBFR1 LS-----SN-ALETKPARKVVADDLVLVTPKKDDFVNVAPSKSKKYKK-KNQQKN----- 361Endotryp EEQERKKREAAAHFDPSQTA-PAGCVVLNDSKWADNKTPYKSTTKLPKQQKKQKEKIPQA 372Paratryp EEEERVKCEAAAKFDPARAA-PSGFVMLGEDKWSSPHSAPKKGGK----KQSAKA--PSA 397Bodo DEEERKRRVAMASFDPSASA-PSGFALAAPIELPKKKSKAA------------------- 374Blechomonas EEEERVKREAAATFDPAKAV-PAGCVVLNEGKWGK-NAKPAPKAS----KKQQKT-NTAP 386T.rangeli EEAELARKQAAAAFDPSKFL-PEGAVLLNDGKKFSDSRKGGAGGK----HKAAQT----Q 393T.cruzi EEVELARKRAAATFDPTNFL-PEGAVLLNDGKKFSEPHKAAPGGK----SKTKQN----Q 386T.grayi EEEELARKNAAATFDPTKFL-PEGAVVLNDGKKWTEPHKAAVGGK----KNKQQQKQQQQ 368T.theileri EEEELARQEAAATFDPTKFL-PEGAVLLNDGKKRTDANKGGALGK----KNKQQQ----Q 388T.vivax EEEERIKKEAVANFDPAKFA-PSGAVILNDGKNWKDHAKGAVGGK----NKQR------P 380T.brucei ENEERARREAAANFDPAEFA-PEGAVVLNDGMSHQNG---GDSKK----QKQQ------A 381T.congolense ENEERAKREAAAAFDPTKFA-PEGAVLLNDGKGAGEG---KSQKS----QH-T------K 381 .

P.parasit ------KTKKASKLVLPL----------AQMEAFSTIGLLPPASAAAVSESLAAVKTKKV 415G.sulph RKTKRKGAAKDSTFSKAVDSEKLPPHNMEYFLAFQKLNVVPPVYVKDILGTIELLKERKS 469ScBFR1 ----TENEQPASIFNKVDGKFTLEP---TLIATLAELDVTVPINSDDVKITVEQLKKKHE 414Endotryp KAGTTLSDQKERPLHHTDEK----------IRLFRIIEIEPTRSRVAIDSTISEIESAKK 422Paratryp KTTEAPVATKDRVLQHSEEK----------QQMFQAVRVDPPLALSAIDAAIKAIEERRK 447Bodo ---PKAEDKTERTVTHNDEK----------KRLFASVGISAPATLSEVEKTIEQLKKKQA 421Blechomonas PVTAAKRVDSTRMLQHPEEK----------MKLFHLIDLEPPITITAFDSTIQAIKAKRK 436T.rangeli KQKPEK-APKNRVLQHPEDK----------IRLFQLVNEELPVALAAIDETMERLRAKQK 442T.cruzi KQKSESAPPKNRVLQHPEDK----------IRLFQLINEEPPVALSAIDGAMETIRSKQK 436T.grayi QQKQQKTEPKNRVLQHGEEK----------IRLFQLIGEEPPLALAAIDDAVKRISAKQK 418T.theileri QQKPKAGTAKNRVLQHSEDK----------IRLFQLINEEPPLALAAIDASVERIRAKQV 438T.vivax TSKKESGASKAVSIKHGEEK----------VELFTLIGEEPPKVLDDIDTLLTKITEKRK 430T.brucei NKAKRDSAPKPRVIKHSEEK----------LELFKLVDEQPPRFLEDIGGIMENIRAKLK 431T.congolense KVNGTESAAKSGVIKHSDEK----------VKLFKLVSEEPPQSVGDIDGVMESLRTKQQ 431

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P.parasit WFNEQTSRPK------------AGKVVEPAAEAAP--------AKVVSPKKKSSKNNKFN 455G.sulph YYENAPE---------------KVDLEATEEEDLS-------------TLLPKSDSDNVI 501ScBFR1 ELLSKQEEQTKQNIESVEKEIEKLNLDYSNKEQQV--KKELEE-KRLKE-------QEES 464Endotryp KYESHIQ---------------TGELVLSSGEDEEDDEENTVNDDEVPSELAPADAQGVV 467Paratryp VMETHIV---------------TGEPVLSSDDENEEENAEETNSPVA---------NGDE 483Bodo EYESHIK---------------TGDLVLSSDDEEEEKEEAAPADE--------------- 451Blechomonas EYESHIT---------------TGDIVLSSESDGEGETHPEDSNLAEPDAAPA------- 474T.rangeli EYESHIK---------------VGELELSSDDEEDEEAQEEEG--AAEATEE----E-AP 480T.cruzi EYESHIK---------------TGELELSSDDEEEEEEQPQEEEETAAAATE----E-VQ 476T.grayi EYESHIK---------------TGDLELSSDDEEEEEQEEPQPQEEKGEEQEV--VDGAK 461T.theileri EYESHKK---------------TGELELSSDDDEEEEEEENVEQEQKEEQNQGDEEQGED 483T.vivax EYASHIK---------------VGELELSSDDDEQEHPQEEEEEAEVEDAVESSKEKGEK 475T.brucei EYSSHIK---------------TGEPELSSDDEEDEEKKEQEGEEEAVA-------KEDE 469T.congolense EYASHIK---------------TGEPELSSDDEEEQEQEQEQEQEQQEN-------EGAA 469. .

P.parasit ASDKDAFPSLGGVAAELPSWGPGMAPAVAEPAVAAEFAEAD---VVTESE- 502G.sulph VEDREDFPDG------LPIVSNGYS-YRAQQGSKPSFAEVMQHSSLTDSVD 545ScBFR1 EKDK------------------EN--------------------------- 470Endotryp VDDA----------------KKDIAE------------------------- 477Paratryp KEEA---EEA------APVLAAA---------------------------- 497Bodo --------------------------------------------------- 451Blechomonas EHNE----EP------VPEVTAE---------------------------- 487T.rangeli KEAD----DV------EE-VTAGAAEAVTQSGQEEATV------------- 507T.cruzi KKKD----EV------EKEVTADKIGLVTQ---NEETVEA----------- 503T.grayi TESQ----LE------ESEVKADE--------------------------- 475T.theileri KE------------------------------------------------- 485T.vivax EEEK----EG------EEKKDEERAE------------------------- 491T.brucei TNEG----EG------EDFA------------------------------- 479T.congolense EEEG----DT------N---------------------------------- 476

ETI45734XP_005709255BFR1EMOLV88_320005100PCON_0018750 BSAL_12610rna_Baya_154_0090TRSC58_05341TcCLB.506525.10 DQ04_02291030TM35_000033410TvY486_1013600 Tb927.10.14150 TcIL3000_10_12030

Phytophthora parasiticaGalderia sulpharariaSaccharomyces cerevisiaeEndotrypanum moterogeiiParatrypanosoma confusumBodo saltansBlechomonas ayalaiTrypanosoma rangeliTrypanosoma cruziTrypanosoma grayi Trypanosoma theileriTrypanosoma vivaxTrypanosoma bruceiTrypanosoma congolense

P.parasitAbbreviation Species Gene ID

G.sulph G.sulphScBFR1

ScBFR1

EndotrypEndotryp

ParatrypParatryp

Bodo Bodo

Blechomonas BlechomonasT.rangeli T.rangeliT.cruzi T.cruziT.grayi T.grayiT.theileri T.theileriT.vivaxT.brucei T.congolense

T.vivaxT.brucei T.congolense

P.parasit