Single-Usecustomised fermenter

July 2014

Scalable platform for microbial fermentation

World first configurable Single-Use-Fermenter

• BactoVesselisavailableinOD110mmsizeforbottomdriveonMagnetic-Stirrer-Table(MST)orintheBio-block

• BactoVesselisavailableinOD130,OD150,OD200withSingle-Use-JacketforHead-Plate-Drive(HPD)orMagnetic-Stirrer-Table(MST)accept-ingmostservomotors

• BactoVesselavailableinOD110,OD130,OD150,OD200,OD250comeswithHead-Plate-Drive(HPD)accept-ingmostservomotors

• BactoVesselissoonavailableinseri-oussize,OD400withStrong-Head-Plate-Drive(SHPD)Configuration

Choose your own design =

fully configurable SUF

Scalable platform =

choose from 2 to >75 litre Vessel Volume

Outstanding performance =

>1,500 rpm capacity

Manufactured entirely from

mono-component rigid plastics

Much more information available on www.cercell.com

Shownafewofthe2,000differentcomponentsfromtheConfigurator Tool abletosatisfyanyrequest

What do I get?

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BactoVessel

Basic specifications for BactoVessel are:• PolycarbonateandNyloncomponents(nomulti-componentplasticfoil!)

• NumberofstandardPG13.5portsaccordingtotheSUFdiameter

• 6differentdiametersand4differentheight

• Turbine(s)forbi-directionalrotation/up-flowcombinedaxial/radialfluidcirculation

• TheSUFbearingsdesigned>1,500rpmforoneweekfermentationprocess

• Baffledvesselforcombinedaxialvortexmixinganddonutshapeflowpattern

•DesignedforNewtonianfluidswithmaxviscosityof50,000cP(similartoyourpreferredstiff,coldgoodmorningyogurt!)

• Designedfor50mBaroperatingpressure

Specification

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Customised5.7litreSUFforHead-Plate-DrivenexttotheRe-Usable-Jacketforcoolingpriortheinstallation.

Fully configurable BactoVessel´s are created by selecting various optional components via the Configurator Tool:• Severalvesselheating/coolingoptionssuchasintegratedorre-usableheatexchanger

• Variousaerationmethods;microporesparger,microporeL-spargers,ringholespargers,L-spargers,headspacegasexchange

• SeveraldifferentfluidIn&Outmethodsandexhaustmethodstoselectfrom

•Arangeofsensors,Single-Use-Sensor(SUS),liquidlevel/foamsensors

•Optionallyoperatingpressureupto500mBarforOD110,OD130andOD150

•Optionallyoperatingpressureupto500mBarforOD200-OD250requiresacovercageincombinationwithRUJ

• DeliveredhighprecisionE-beam(32kGy)irradiatedindualpolyesterfoilbags

• AllcomponentsinmediacontactareISO10993,FDAapprovedorUSPclassVIcertifiedmaterials.

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BactoVessel facilitate a range of different radial mixing turbine principles: Rushton, Jacob, Smith, Hjort and Bakker:

•OuterDiameter,ODmm:40,50,60,70,80,90,100and120

•Numberofbladesare6or8dependingonprinciple

• Bladeshapes:flat,symmetrichalfcircle,symmetricconcav,asymmetricconcav

• Jacob´sflatbladeangledin75degreefromvertical

Heightofbladesisfixedaccordingtotheturbinediameter.DimensionofturbineandbladedesignedselectablefromtheConfigurator Tool.IfoneormoreaxialmixingMarineimpellersisrequiredintheBactoVesselsuchisavailableinaselectablediameter,3blade,16degreebladeangleddesign.

Two identical BactoVessel OD150 mmintegrating3baffles,ringspargerandRushtonandSmithturbines.Thetwoturbinesperformsverydifferent!

Agitation in BactoVessel Turbines

Unlimited range of turbines

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BafflesBaffles are obstructing vertical arranged vanes or elongated plates inside the vessel needed to stop the radial swirl inside the fermenter and convert the rotational flow to axial mixing.Withoutbaffles,thetangen-tialvelocitiescomingfromanyturbine(s)causestheentirefluidmasstospincreatingacentralvortex.Baffles,sotospeak,increasethefrictiontothevesselinnerwallsurface.BactoVesselbafflesarestraightflatplatesverticallyandperpendicularorientedclosetotheinnersideofthevesselattachedtoandextendingfromthebottomuptoaboveliquidsurface.

NumberofbafflesinBactoVesselrangefrom3to6dependingonthefermenterdiameter.Thebafflewidth(B1)istypicallyT/10-T/12ofthevesselinnerdiameter(T1).BafflesarelocatedwithdistanceofT/72–T/50fromthewall.

OD130 x 420 mm BactoVessel customisedtoend-userrequirementintegrating3baffles,ringsparger,dualIn&Out,HPDdriveandtwospecificRushtonturbines.

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Since oxygen is sparingly soluble in water, it’s one of several growth-limiting factor in fermentations. Thoughdealingwithaerobicmicro-bialapplicationsithelpsthatthecells,bacteria,yeast,etcarealmostinde-structiblefacilitatinghighRPM,suchasupto>1,500RPMwithBactoVessel.

Aeration in BactoVesselThe Rushton 100 % radial flow pattern turbine are a most cost effective design with performance generally limited by pockets or cavities, which cling to the back of the blade at high gas flow rates and/or RPM.Rushtoncausecavitation,considerablestreamliningandpowerdrawreduction.Whengasflowgetstoohigh,thepocketscanbridgebetweenblades,resultinginfloodingandseveremechanicalinstability.RushtonRT8hasthehighestun-gassedPowerNumberofanycommonturbine.

Smithturbinesisahighefficiencyagitatorforveryhighgasdispersionapplications.Charac-terizedbysymmetricalcurvedpipesectionsmountedtoarounddiscresultinginlowPowerNumberandincreasedefficiency.ThisparticularSmithmodelST6hashalftheun-gassedPowerNumbercomparedtotheRushtonturbine.

Ring sparger

Two photosshowaOD150x320mmBactoVesselequippedwiththeadvancedOD60Smith,6bladesturbinedesignplusMarineimpeller,3baffles,1nl/minWVgasinjection.

• At 0 RPM under static condition

• At 1000 RPM. Its worth to notice the limited foam generation even at such high RPM. Head space is in general free of foam

0 RPM

1000 RPM

BactoVessel facilitate aeration / gas injection throughaseriesof0.5mmholesarrangedinacirclebelowtheprimaryturbineintegratedasapartofthevesselbottom.NumberofholesdependingontheturbinediameterorchoicesthroughtheConfigurator Tool.

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Top class kLa values

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This Smith symmetrically arranged curved (half pipe) blade design from the 1980s is able to handle increased

injected gas volume. CandispersetwicethegascomparedtotheRushtonbeforeflooding.Doesnotexperienceasgreatapowerdrop-offduetogas

loadingcomparedtotheRushtonturbine.Recommendedcombinedwithanup

pumpingMarineimpeller.

Revolutions

ExhaustSingle-Use-Gas-Cooler are available in three sizes and combines inner boro silicate glass tube for excellent heat transfer and outer PC tube and cooling liquid connections. Alltrans-parenttubesallowtheusertoinspectthefunctionality.

Large size –indesignphase.Max100nl/min/37°gasvolumecapacity.

ConfigurationviatheConfigurator Tool

Small size–ID/OD8/12x270mmglasstube,lowerendinletandoppositeoutletendfittedwithID/OD10/14mmsiliconehose,outletholdingoneormore26cm2surfaceareasterilefilter.Coolingarea68cm2suitableformax2nl/min/37°gasvolumecapacity.

Medium size–ID/OD24/30x300mmglasstube,lowerendinletID/OD18/22mmsiliconehose,andoutletfittedwithID10/14mmsiliconehosepieceand400cm2surfaceareafilter.GlasstubefilledwithRaschig6x6mmglasstubeelements.Coolingarea500cm2suitableformax20nl/min/37°gasvolumecapacity.Coolingliquidin/outletbeingG1/8”forID6mmhoseconnection.

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Sensors and sampling

Sampling

Even the shown photo is a CellVessel bio-reactor the In&Out system for BactoVessel share the same principles and specifications. ViatheConfigurator Tool youcanchooseanycombinationofhoses,connectorsforfluidspassingIn&OutoftheBactoVesselheadplate.AsIn&OutrangeofaccessoriesisbasedonthePG13.5pluganywishispossibletofullfil

Unlimited possibilities in connections

The CellVessel bioreactor use

thesameIn&Outsystem

specificationastheBactoVessel.

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Single-Use-SensorsBenefits:

• IntegratedSUSreducecontaminationrisk

• Saveshoursofpreptimeandlabor,asnoautoclavingorsterilisationisneeded

• EnablesSUSsetuprightonthebenchtop–nobiosafetycabinet/hoodneededforoperation

•Noautoclavingfacilityisneeded

• ChooseSUSviaConfigurator Tool

Single-Use-Sensors (SUS) offer many advantages

Express

installation

Much more information available on www.cercell.com

Bio mass sensors

• Capacitivesensingtechnologyallowspreciseon-linemonitoringofthecellmass,viablecelldensityaswellascellphysiologicalstate.

• Userscanalsotrackcellcyclechanges,modelapoptosis,andpredictproteintiterallinrealtime

DO Single-Use-Sensor

• Non-invasiveDOwellforVisiFerm™.Pre-installedwithSUScapintegratedtotransparentPCtube.

• ExtendsDOsensorlife,assensorisneverautoclaved

•DOsensordesignedwithdigitalsignalcommunicationthatfitsmostPCS

pH Single-Use-Sensors

• Pre-installed6monthdrystoragetolerantpHSUSsuchasOneFermorFermProbe

• ClassicaldimensionpHsensorwithextendedshelflifetimeofferingmonthsofoperation

• ClassicalpHsignalfitsanyPCS

•Availablecombinedwith°CanalougeoutputfordualdigitalsignalcommunicationviaARCmodule

ARC moule for SUS

• Pre-installedpHSUSOneFermVP6designedforconnectiontoARCanalogue/digitalmodule

• Availablecombinedwith°CanalougeoutputfordualdigitalsignalcommunicationviaARC

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BactoVessel in RUJWater conveying Process-Control-System (PCS) benefit from the RUJ heat exchanger for BactoVessel. TheRUJdesignaddsignificantsupportfacilitatingtheservomotormountedontheheadplate(HPD).RUJisfurtherrequiredforSUFoperatingpressureupto500mBar.

OD150x320 BactoVessel designedwithturbineandimpeller.SUFdesignedformicrobialapplicationwhereexothermalmicrobialreactionrequirescooling.

TheRUJmountedwitha5.7litreVVSUFwith2.5litreWVareabletoremoved~20watt/litremediapower.TheRUJallowfastturn-overtimeandhighestpossibleexchangeofenergy.ShownwithRectus21couplingsfordirectconnectiontoBioStatsetoforangehoses.

PreparationBactoVessel SUF weigthislessthan1/10oftheglass/steelSTRofcomparablevolume.AnyonecaneasilytumbletheSUFinasafeway.

The BactoVessel concept is to move weight from the STR, Stirred-Tank-Reactors made from glass / Stain-less Steel to “fixed” components. ThetraditionaljacketedStirred-Tank-Reactorshaswith5.7litreVVtypicallyaweightof18kilo.Notreallyeasytohandle!

TheBactoVesselSUFiskeptaslightaspossible.The5.7litreBactoVesselSUFweigthislessthan2kilo!Alltheweigthismovedtothefixedcomponent–RUJ!

Reduced

turn-around

time

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Heating & cooling with Re-Usable-JacketFor water conveying Process-Control-System (PCS) CerCell offer a stand-alone Re-Usable-Jacket (RUJ) accessory to insert the SUF. ThisjacketisanefficientheatexchangerandanadvancedoptionforSUFOD110–250mmandin225,320and420mmheight.AflexibleO-ringsealbetweentheremovableSUFtopflangeandsupportringallowsimplereplacementoftheSUF.SuchSUFareequippedwithhead-plateflangeasselectedintheConfigurator Tool.

Specifications for RUJ:

• TheRUJisoneofmanyaccessoriesfoundonwww.cercell.com/commerce

•Operatingtemperature5-60°C

•Maxjacketoperatingpressure400mBar–RUJequippedwithaspringloadedover-pressure,systemprotectionvalvesetat500mBar

• Recommendedwaterflow1litre/minperlitre/SUFWV

• TwoG3/8”femalethreadforIn/Outadapt-ers:OD10barborGL18maleglassthread(similartoUniVesselandothers)orRectus21couplings(similartoBiostatsetup)

•Weightranging5-10kilodependingonsize,theweightfacilitatesstablearrange-mentondesktop.

• Thetransparentrigidplastictubematerialgivesexcellentprocessview.

Water connections

ProudProduction ManagerwithBactoVesselinRUJ

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Fromlefttorigth:Rectus21toG3/8”coupletkit,G3/8”toOD10barbkit,G3/8”toGL18glassthreadkit,hoseadapterstoGL18kits

For water conveying Process-Control-System (PCS) theoptionalintegratedSingle-Use-Jacket(SUJ)heatexchangerisanefficientoptionfortheBactoVesselfermenterfamily.AllowtheuseofMSTupto13.3litreSUFsize.

Specifications:

• SelectedviatheConfigurator Tool

•Operatingtemperature5-60°Cheating/coolingwater

• Recommendedmaxoperatingpressureis500mBar

• Burstpressureat20°Cis<1Bar

• Recommendedcoolingflow1litre/min/WV

• StandardtwoG3/8”femalethreadcon-nectionsonjacket

• Adaptersavailableasaccessory:G3/8”toOD10barb,G3/8”toGL18maleglassthreadadapterlikeUniVessel,G3/8”toRectus21male/femalekit

• Thetransparentrigidselfsupportiveplas-ticmaterialgivesexcellentprocessview.

Heating / cooling with Single-Use-Jacket

16.4 litre VV CellVessel (OD250x320) viaConfigurator Tool equippedwithSUJ.ThetwofemalethreadedG3/8”connectionsarevisibleontherightsideoftheSUF.

Water connection

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BactoVesselequippedwithSIngle-Use-JacketandfemaleG3/8”threadedconnectionsforavarietyofadapters.

BactoVessel with SUJ

5.7 litre VV BactoVessel viaConfigurator ToolequippedwithSUJ

1. FHC–Flexible-Heating-CoolingelementwrappedaroundtheSUFwith5.5litreVV,2.5litreWV,removedpower~5watt/litremedia

2. AluminiumRHScoilencapsulat-ingtheSUFwith5.5litreVV,2.5litreWV,removedpower~10watt/litremedia

3. IntegratedSingle-Use-JacketonSUFwith5.5litreVV,2.5litreWV,removedpower~20watt/litremedia

4. IntegratedjacketonUniVesselSTR-glass/steel,removedpower~25watt/litremedia

Heat exchange

Cooling

as with

glass

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HPD drive• Head-Plate-Drive(HPD)forBactoVesselSUFoffercoupling/adapterkitstoservomotorsfromApplikon,Biostat,DasGip,BJ,NBS,Solaris,Finesse,BBI-biotechandotherPCSservomotors

• Re-usableservomotoradapterseasilyattachedtotheOD30mmHPDcentrebearingontheBactoVesselheadplate

• Forhighloadsamotorstandishelpful

BactoVessel 5.7 litrefacilitatingSmithturbineandMarineimpelleroperatingat1,500RPMmountedinRe-Usable-Jacket,RUJequippedwithservomotorsupportandSky-Supportforthegas-coolerandexhaustfilter.

Agitation methods

Kollmorgen AKM23DservomotordrivingviaHPDinBiostatsetupa5.7litreBactoVesselsubmersedinaRUJ

Use you

own PCS

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MST drive•Magnetic-Stirrer-Table(MST)Stain-lessSteelconstructionfeaturinglonglifeandsilentrunningtoothedrubberbeltonaluminiumsprock-ets,twolifetimesealeddouble-rowballbearings

•MSTrotor(undertheSUForSUB)isequippedwith12permanentmag-netsandabletotransfer>100wattpowerdependingontheparticularservomotorcapacity

• Dim:390x180x50mm,weightis5,7kilo

• ForBactoVesselwithSIngle-Use-Jacket,SUJ

MSTdrivinginthiscasea3.0litreCellVesselbioreactor.FullyindependentoftheparticularPCSservomotor.TheMSTadapter,seentotheleft,takescareofthisisssue.

Kollmorgen AKM23D servomotordrivingMSTinBiostatsetup Mostservomotorsfacilitatetheseadapters.

You get drive options

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SUF productsOur “Configurator Tool” can help you design a SUF to your User-Requirement-Specification.

BactoVessel sizes to select from

Vessel diameter, OD mm 110 130 150 200 250 400

Number of PG13.5 ports 7 7 9 10 10 12

Turbine diameter, OD mm 40-50 50-60 50-70 80-100 100-120 100-150

Vessel Volume, litre

225 mm 2.1 3.0 4.1

320 mm 4.2 5.7

420 mm 13.3 21.0

500 mm

600 mm 29.5 75.6

Specificationssubjecttochangewithoutnotice.CerCell,CellVessel,BactoVessel,Applikon,Biostat,DasGip,Finesse,Solaris,Hamilton,Visiferm,Presensareregistratedtrademarks.

CerCellApsMalmmosevej19CDK-2840HolteDenmarkwww.cercell.comphone+4545423393info@cercell.com

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