Supplementary Figure 1. Aggregation state of A1-42 was measured by thioflavin T fluometric

assay in a cell-free system. Thioflavin T spectroscopic assay was carried out from 0 to 5 days at 37 C. After A1-42 or non-containing samples (0 – 5 days) were added to 50 M Thioflavin-T solution, fluo-rescence was measured at 450 nm excitation and 482 nm emission using a fluorescence spectrome-ter. Each sample was determined in triplicates.

Supplementary Figure 1

0 1 2 3 4 50

200

400

600

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1000 A1-42Control

(Day)

Th

iofl

avin

Tfl

uo

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ce

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rmalized

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ntr

ol)

A