Swine Influenza Panel ( 신종플루 )CDC Swine Flu Test Kit Incorporates Biosearch Technologies’ Black Hole Quencher(BHQ) Dye

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Responding to a Public Health Emergency

In response to the outbreak of swine flu, or 2009 H1N1, the declaration of a public health emergency by the Secretary of Health and Human Services, and requests by the US Center for Disease Control and Prevention’s (CDC), the US Food and Drug Administration (FDA) issued an Emergency Use Authorization (EUA) for the emergency use of the Swine Influenza Virus Real-time RT-PCR Detection Panel (Swine Flu Test Kit).  The CDC readily developed the Swine Flu Test Kit to detect and identify cases of the swine influenza A (H1N1) virus, and is the only FDA-authorized distributor of this molecular diagnostic test to public health and other qualified laboratories.

Diagnosing the Swine Flu - BHQ Dye to Detect Swine Influenza Strains

The Swine Flu Test Kit is a panel of dual-labeled probes and primers for use in real-time PCR to detect swine flu RNA in nasal and/or nasopharyngeal swab specimen.  The CDC designed InfA and swInfA (NP gene) probe and primer sets to universally detect all swine influenza A strains as well as a swH1 (HA gene) probe and primer set to universally detect the swine H1 influenza strain.  Available to the public online, the CDC posted its protocol of real-time PCR for detecting swine influenza A/H1N1 (CDC REF.#I-007-05), which lists FAM and Biosearch Technologies’ Black Hole Quencher® (BHQ®-1) dye as the official fluorophore and quencher labels for the Swine Flu Test Kit.

As the inventor of the Black Hole Quencher dyes, Biosearch Technologies is the sole manufacturer of the true dark quencher currently used for labeling probes implemented in swine flu diagnostic kits.

Order Information – 미국 CDC 권고 Swine Flu Realtime PCR Primer & Probe VAT 별도

Cat. No Swine Flu Panel 보장량 5'Intern

al3'

Purification

Price

INFA-P-5 Universal Influenza A Probe 5 nmol FAM 　 BHQ-1 Dual HPLC 240,000INFA-F-20 Universal Influenza A Forward Primer 20 nmol 　 　 　 RPC 30,000INFA-R-20 Universal Influenza A Reverse Primer 20 nmol 　 　 　 RPC 30,000SWINFA-P-5 Swine Influenza A Probe 5 nmol FAM BHQ-1 Spacer 3 Dual HPLC 280,000SWINFA-F-20

Swine Influenza A Forward Primer 20 nmol 　 　 　 RPC 30,000

SWINFA-R-20

Swine Influenza A Reverse Primer 20 nmol 　 　 　 RPC 30,000

SWH1-P-5 Swine Influenza H1 Probe 5 nmol FAM BHQ-1 Spacer 3 Dual HPLC 280,000SWH1-F-20 Swine Influenza H1 Forward Primer 20 nmol 　 　 　 RPC 30,000SWH1-R-20 Swine Influenza H1 Reverse Primer 20 nmol 　 　 　 RPC 30,000RNP-P-5 Human RnaseP Positive Control Probe 5 nmol FAM 　 BHQ-1 Dual HPLC 240,000

RNP-F-20Human RnaseP Positive Control Forward Primer

20 nmol 　 　 　 RPC 30,000

RNP-R-20Human RnaseP Positive Control ReversePrimer

20 nmol 　 　 　 RPC 30,000

한국공식대리점 부경에스엠

Real Time qPCR TaqMan Probe

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PCR Primer Pairs

TaqMan Probe 구매시 적용

Dual HPLC Purification

Multiplexing Recommendations for Dual-labeled Dark-Quenched Probes & Primers

BHQTM Quencher & Dye Selection Chart

기타 규격 및 자세한 제품 정보는 w w w .biosearchtech.com 에서 확인 하실 수 있습니다

Cat NO Description Scale Yield Cat NO Description Scale YieldDLO-RFB-5

5' FAM / 3' BHQ-1 50nmol 10nmol DLO-TB2-5

5' TAMRA / 3' BHQ-2 50nmol >10nmol

DLO-FB1-5 100nmol >10nmol DLO-TB2-2 200nmol >25nmol DLO-FB1-2 200nmol >25nmol DLO-TB2-1 1µmol >60nmol

DLO-FT-5 5' FAM / 3' TAMRA

100nmol >10nmol DLO-C590B2-

5 5' CAL Fluor Red 590 (TAMRA) / 3' BHQ-2

50nmol >10nmol

DLO-FT-2 200nmol >25nmol DLO-C590B2-

2200nmol >25nmol

DLO-F(T-B1)-2

5' FAM / Internal T-BHQ-1 200nmol >10nmol DLO-Q5B2-5 5' Quasar 570(Cy3) / 3’ BHQ-2

50nmol >10nmol

DLO-F(T-B1)-1

/ 3’ C3 1µmol >30nmol DLO-Q5B2-2 200nmol >25nmol

DLO-TET-5 5' TET / 3' TAMRA

50nmol >10nmol DLO-Q6B2-5 5' Quasar 670(Cy5) / 3’ BHQ-2

50nmol 10nmol DLO-TET-2 200nmol >25nmol DLO-Q6B2-2 200nmol >20nmol

DLO-TEB1-5 5' TET / 3' BHQ-1

50nmol >10nmol DLO-Q7B2-5 5' Quasar 705(Cy5.5) / 3’ BHQ-2

50nmol >3nmol DLO-TEB1-2 200nmol >25nmol DLO-Q7B2-2 200nmol >10nmol DLO-CGB1-5 5' CAL Fluor Gold 540 (TET,

JOE) / 3' BHQ-1

50nmol >10nmol DLO-CAB2-5 5' CAL Fluor Red 610 (TEXAS RED)

/ 3' BHQ-2

50nmol >10nmol

DLO-CGB1-2 200nmol >25nmol DLO-CAB2-2 200nmol >25nmol

DLO-HB1-5 5' HEX / 3' BHQ-1

50nmol >10nmol DLO-C635B2-

5 5' CAL Fluor Red 635 (LC Red 640)

/ 3' BHQ-2

50nmol >3nmol

DLO-HB1-2 200nmol >25nmol DLO-C635B2-

2200nmol >10nmol

DLO-COB1-5 5' CAL Fluor Orange 560 (VIC,HEX) / 3' BHQ-1

50nmol >10nmol DLO-B2P6-2 5’ BHQ-2 / 3’ Pulsar 650

200nmol >10nmol DLO-COB1-2 200nmol >25nmol DLO-B2P6-1 1µmol >40nmol

Cat NO Description Scale YieldP2-5

Primer Pair (Cartridge Purified, up to 30 base)

50nmol >11nmol P2-2 200nmol >45nmol P2-1 1µmol >225nmol

InstrumentCalibration required

Multiplexing Degree

DYE 1 DYE 2 DYE 3 DYE 4 DYE 5

Prism 7700 YES Duplex FAM CAL Fluor Gold 540 SuperROX 　 　Prism 7900 YES Duplex FAM CAL Fluor Gold 540 SuperROX 　 　Prism 7000 YES Duplex FAM CAL Fluor Gold 540 SuperROX 　 　Prism 7300 YES Duplex FAM CAL Fluor Orange 560 SuperROX 　 　Prism 7500 YES 4-Plex FAM CAL Fluor Orange 560 TAMRA SuperROX Quasar 670

iCycler iQ YES 4-Plex FAM CAL Fluor Orange 560CAL Fluor Red

610Quasar 670 　

iQTM 5 YES 5- Plex FAM CAL Fluor Gold 540CAL Fluor Red

590CAL Fluor Red

610Quasar 670

SmartCycler YES Triplex FAM CAL Fluor Orange 560CAL Fluor Red

635　 　

SmartCycler II YES 4-Plex FAM CAL Fluor Orange 560CAL Fluor Red

610Quasar 670 　

Rotor-Gene 3000

NO 4-Plex FAM CAL Fluor Orange 560CAL Fluor Red

610Quasar 670 　

Rotor-Gene 6000

NO 5- Plex FAM CAL Fluor Orange 560CAL Fluor Red

610Quasar 670 Quasar 705

MasterCycler YES Duplex FAM CAL Fluor Gold 540 　 　 　Opticon 2 YES Duplex FAM CAL Fluor Orange 560 　 　 　

Chromo 4 YES 4-Plex FAM CAL Fluor Orange 560CAL Fluor Red

610Quasar 670 　

MX3000P NO 4-Plex FAM CAL Fluor Orange 560CAL Fluor Red

610Quasar 670 　

MX4000 NO 4-Plex FAM CAL Fluor Orange 560CAL Fluor Red

610Quasar 670 　

LightCycler 1.2 YES Duplex FAM Pulsar 650 　 　 　LightCycler 2.0 YES Triplex FAM CAL Fluor Red 610 Pulsar 650 　 　

LightCycler 480 YES 4-plex FAM CAL Fluor Orange 560CAL Fluor Red

610Quasar 670 　

　Determined through research at Biosearch

　 Determined through research of a collaborator

　 Predicted based on instrument specifications