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Vol 17, No 3 September 2016
Terakreditasi Dirjen Dikti
S.K. No. 81/DIKTI/Kep/2011
pISSN: 1411-8327; eISSN: 2477-5665Website : ojs.unud.ac.id
Jurnal Tiga BulananJurnal Veteriner
Jurnal Kedokteran Hewan Indonesia
(Terbit : Maret, Juni, September, Desember)
depan 1
PEMIMPIN UMUM : I WAYAN BATAN DEWAN REDAKSI : NYOMAN MANTIK ASTAWA (KETUA), IDA BAGUS ARKA,NYOMAN SADRA DHARMAWAN, IWAN H. UTAMA, I GUSTI NGURAH KADE MAHARDIKA, I KETUT PUJA, I KETUTSUATHA, TJOK GDE OKA PEMAYUN, ROOSTITA L. BALIA, I KETUT BERATA, REDAKTUR PELAKSANA : I NYOMANSUARTHA & I G M KRISNA ERAWAN, SEKRETARIS REDAKSI : I NYOMAN SUARSANA, STAF REDAKSI : I WAYANSUARDANA, I GUSTI NGURAH SUDISMA, AIDA LOUISE TENDEN ROMPIS, NI GUSTI AGUNG AYU SUARTINI, I MADE SUKADA,ANAK AGUNG SAGUNG KENDRAN, ANAK AGUNG AYU MIRAH ADI, I MADE KARDENA, YANA QOMARIANA.TATA USAHA : PUDJI RAHARDJO, WERDI SUSARI. KANTOR REDAKSI & ALAMAT SURAT : KLINIK HEWAN FKH UNUD,Jl. Raya Sesetan Gg. Markisa 6 Banjar Gaduh, Denpasar - Bali. PENERBIT : FAKULTAS KEDOKTERAN HEWAN, UNIVERSI-TAS UDAYANA BEKERJA SAMA DENGAN PERHIMPUNAN DOKTER HEWAN INDONESIA. REKENING : NOMOR 0118628705A/N drh. I NYOMAN SUARTHA, M.Si BNI CABANG DENPASAR DICETAK OLEH: PERCETAKAN PELAWA SARI, JL. ANTOSURA33 DENPASAR
Setiap naskah yang dikirim ke redaksi untuk dipublikasikan dalam
Jurnal Veteriner dipandang sebagai karya asli penulis dan bila diterima, naskah
tersebut tidak diperkenankan dipublikasikan lagi secara keseluruhan ataupun
sebagian tanpa seijin Jurnal Veteriner.
Di Kalimantan berdasarkan wilayahnya tersebar tiga subspesies orang utan. Orang utan subspecies
Pongo pygmaeus pygmaeus ada di barat laut; Pongo pygmaeus wurmbii ada di barat dan di tengah; dan
Pongo pygmaeus morio ada di timur. Status mereka pada tahu 2016 dinyatakan kritis (terancam punah)
oleh Badan Konservasi Dunia, mengikuti saudaranya orang utan Sumatra Pongo abelii. Yang membuat
mereka nyaris punah, karena kebanyakan dari mereka hidup di kawasan non konservasi yang ditetapkan
sepihak oleh manusia. Mereka kini hidup pada hutan produksi, konsesi batubara, konsesi kelapa sawit,
kebun warga, dan sempadan sungai. Mereka renta berkonflik dengan saudara satu kelasnya, manusia
(Homo sapiens). Oleh manusia yang beriman (?) mereka biasa dijebak, diperangkap, ditangkap, dan
diperdagangkan. Walau pun sebenarnya kita sudah memberi perhatian kepada mereka, nampaknya apa
yang telah kita berikan, sepertinya itu semua belum cukup.A
nd
rew
Su
ryon
o
depan 2
KerjasamaFakultas Kedokteran Hewan Universitas Udayana& Perhimpunan Dokter Hewan Indonesia, Jakarta
MITRA BESTARI JURNAL VETERINER
Prof. Dr. drh. Sri Subekti DEAGuru Besar Parasitologi Fakultas Kedokteran Hewan Universitas Airlangga Surabaya
Prof. drh. Hastari Wuryastuti,MSc., PhDGuru Besar Nutisi Klinik Fakultas Kedokteran Hewan Universitas Gadjah MadaYogyakarta
Prof. drh. R. Wasito,MSc., PhDGuru Besar Patologi Fakultas Kedokteran Hewan Universitas Gadjah MadaYogyakarta
Prof. Ir. D.K.HaryaPutra,MSc.,PhD.Guru Besar Biologi Reproduksi Fakultas PeternakanUniversitas Udayana Denpasar
Prof. Dr. I Wayan Teguh WibawanAhli Imunologi, Mikrobiologi, Patologi Fakultas Kedokteran Hewan Institut Pertanian Bogor
Drh. Dewa Made Ngurah Dharma,MSc., PhD.Ahli Patogi Balai Besar Veteriner Denpasar
Prof. Dr. Ir Ika MustikaProfesor Riset, Ahli Nematologi Balai Penelitian Tanaman Tropis, Cimangu Bogor
Prof. Ir. Wasmen Manalu,PhDGuru Besar Fisiologi dan Farmakologi Fakultas Kedokteran Hewan IPB Bogor
Prof. drh. Adji Santoso Dradjat, BSc Vet. Mphil., PhD.Guru Besar Reproduksi Ternak Fakultas Peternakan Universitas Mataram
Prof (Riset) Dr Supar, MS, APUAhli Bakteriologi Balai Besar Penelitian Veteriner Bogor
Dr Nastiti KusumoriniAhli Neurofisiologi Fakultas Kedokteran Hewan IPB Bogor
Drh Fadjar Satrija, MSc., PhDAhli Parasitologi Veteriner Fakultas Kedokteran Hewan IPB Bogor
Prof. Dr. Fedik Abdul RatamGuru Besar Virologi dan Imunologi Fakultas Kedokteran Hewan Universitas Airlangga
Prof . Dr. drh. Siti Isrina Oktavia salasiaGuru Besar Patologi Klinik Fakultas Kedokteran Hewan Universitas Gadjah Mada Yogyakarta
Drh. Sulistiyani, MSc., PhDAhli Molekular dan Selular Patobiologi Fakultas MIPA IPB Bogor
Prof. drh Arief Boediono, PhDGuru Besar Embriologi Fakultas Kedokteran Hewan IPB Bogor
Dr. Agus WiyonoAhli Virologi, Sub Direktorat Perlindungan Hewan Dirjenak Deptan Jakarta
Naskah AsliOriginal Article
WYANDA ARNAFIA, SITI GUSTI NINGRUM, ERFIANDINI EKA PUSPITA,DENNY WIDAYA LUKMAN, FACHRIYAN HASMI PASARIBU, I WAYAN TEGUH WIBAWANCross Reaction of Serum in Salmonella enteritidis-Vaccinated Chicken to Some Salmonella enterica Serotypes(REAKSI SILANG SERUM AYAM YANG DIVAKSIN DENGAN SALMONELLA ENTERITIDIS TERHADAP BEBERAPA SEROTIPE SALMONELLA ENTERICA) ............................ 316-321
RISA INDRIANI, NI LUH PUTU INDI DHARMAYANTIPengembangan Vaksin Inaktif TeteloGenotipe VII Isolat Lokal pada Kondisi Laboratorium.(DEVELOPMENT OF TETELO INACTIVATED VACCINEGENOTYPE VII LOCAL ISOLATE IN LABORATORY CONDITION) ................................................... 322-330
ANDIKA BUDI KURNIANTO, GUSTI AYU YUNIATI KENCANA,I NYOMAN MANTIK ASTAWARespons Antibodi Sekunder Terhadap Penyakit Tetelo pada Ayam Petelur PascavaksinasiUlangan dengan Vaksin Tetelo Aktif(NEWCASTLE DISEASESECONDARY ANTIBODY RESPONSEAFTER REVACCINATION IN LAYER WITH THE ACTIVE ND VACCINE) ........................................ 331-336
WAHONO ESTHI PRASETYANINGTYAS, DENY PUTRA ROMADHON, FITRI SUSANA,ITA DJUWITA, KUSDIANTORO MOHAMADBlack Seed (Nigella sativa) Extract Induce in Vitro Proliferationand Differentiation of Rat Pancreatic and Bone Cells(EKSTRAK JINTAN HITAM (Nigela sativa) MENGINDUKSI PROLIFERASIDAN DIFERENSIASI SEL PANKREAS DAN SEL TULANG TIKUS SECARA IN VITRO) ................. 337-346
WORO HASTUTI SATYANTINI, AGUSTONO, ARIMBI,EMY KOESTANTI SABDONINGRUM, MYRNA BUDI, LINA WAFIA ASMIPeningkatan Respons Imun Non Spesifik Ikan Gurame PascapemberianEkstrak Air Panas Mikroalga Spirulina platensis(ENHANCEMENT OF NON-SPECIFIC IMMUNE RESPONSE OF OSPHRONEMUS GOURAMYAFTER GIVING OF HOT WATER EXTRACT SPIRULINA PLATENSIS) ............................................. 347-354
KHAIRUN NISAA, SUKENDA, MUHAMMAD ZAIRIN JUNIOR,ANGELA MARIANA LUSIASTUTI, SRI NURYATIBenih Keturunan Induk Ikan Nila yang Divaksinasi pada Tingkat Kematangan Gonad-2Lebih Tahan Terhadap Infeksi Streptococcus agalactiae(RESISTANCE OF FRY FROM TILAPIA (OREOCHRIMIS NILOTICUS) VACCINATIONBROODSTOCK AT DIFFERENT GONADAL DEVELOPMENTAL STAGESTO STREPTOCOCCUS AGALACTIAE INFECTION) ................................................................................ 355-364
NINING ARINI, MIRNAWATI SUDARWANTO, IDWAN SUDIRMAN, AGUSTIN INDRAWATIPemanfaatan Supernatan Lactobacillus plantarum Sebagai PenghambatPertumbuhan Escherichia coli pada Dangke Susu Sapi(UTILIZATION OF LACTOBACILLUS PLANTARUM SUPERNATANAS AN INHIBITIOR OF ECHERICHIA COLI GROWTH IN COW’S MILK DANGKE .......................... 365-373
KORBINIANUS FERIBERTUS RINCA, TJOKORDA SARI NINDHIA,I WAYAN SUARDANAFaktor-faktor Risiko Penyebaran Escherichia coli O157:H7pada Sapi Bali di Kuta Selatan, Badung, Bali(RISK FACTORS FOR DISSEMINATION OF ESCHERICHIA COLI O157:H7IN BALIN CATTLE IN SOUTH KUTA, BADUNG, BALI) ......................................................................... 374-382
TRI NURHAJATI, KOESNOTO SOEPRANIANONDO,WIDYA PARAMITA LOKAPIRNASARIUji Aktivitas Pertumbuhan Enterobacter cloacae Selulolitik Aerob Rumen-1 IsolatAsal Limbah Cairan Rumen Sapi Peranakan Ongole(GROWTH ACTIVITY ASSAY OF CELLULOLYTIC BACTERIA ENTEROBACTER CLOACAESAR 1 (CELLULOLYTIC AEROB RUMEN 1) ISOLATED FROM ONGOLE CROSSBREEDBOVINE RUMEN FLUID WASTE) ................................................................................................................. 383-388
I GUSTI AYU DEWI RATNAYANTI, NI PUTU SRIWIDYANI, I DEWA AYU INTEN PRIMAYANTI,I GUSTI KAMASAN, NYOMAN ARIJANA, I GUSTI NYOMAN SRI WIRYAWAN,IDA AYU IKA WAHYUNIARI, I WAYAN SUGIRITAMA, I GUSTI NGURAH MAYUNGrowth Hormone Menurunkan Ekspresi Protein p53 dan p21 Sel Endotel Tikus Jantan(GROWTH HORMONE REDUCES P53 AND P21 ENDOTHELIALPROTEIN EXPRESSION IN MALE RAT) .................................................................................................... 389-395
DAFTAR ISIVol 17, No. 3 September 2016
Terakreditasi Dirjen DiktiS.K. No. 81/DIKTI/Kep/2011
pISSN: 1411-8327; eISSN: 2477-5665Website : ojs.unud.ac.id
Terbit sejak 18 Desember 2000
Jurnal VeterinerJurnal Kedokteran Hewan Indonesia Indonesian Veterinary Journal
depan 3
DAFTAR ISI (Lanjutan)Vol 17, No 3 September 2016
Terakreditasi Dirjen Dikti
S.K. No. 81/DIKTI/Kep/2011
pISSN: 1411-8327; eISSN: 2477-5665Website : ojs.unud.ac.id
Terbit sejak 18 Desember 2000
Jurnal VeterinerJurnal Kedokteran Hewan Indonesia Indonesian Veterinary Journal
depan 4
NOVI SUPRIHATIN, LIGAYA ITA TUMBELAKA, MOHAMAD AGUS SETIADIProfil Progesteron Air Susu dan Tingkat Kebuntingan Sapi Perah PascasinkronisasiEstrus Menggunakan Prostaglandin F
2Alfa atau Progesteron-CIDR
(MILK PROGESTERONE PROFILE AND PREGNANCY RATE ON DAIRY CATTLEAFTER ESTROUS SYNCHRONIZATION WITH PROSTAGLANDIN F
2
ALFA OR PROGESTERONE-CIDR) .............................................................................................................. 396-403
RINI WIDYASTUTI, SONY HERU SUMARSONO, ARIEF BOEDIONO, SITI DARODJAH RASADLow Concentration of Ethylene Glycol Improve Recovery Rate of HumanSpermatozoa After Vitrification(ETILEN GLIKOL KONSENTRASI RENDAH MENINGKATKANRECOVERY RATE SPERMATOZOA MANUSIA PASCAVITRIFIKASI) ................................................. 404-410
YULIANA, SIANNY HERAWATIPhytochemical Content and Protective Effect of Kleinhovia hospital Leaves Extracton Pancreatic Cytotoxicity in Hyperglycemic Rats(KANDUNGAN FITOKIMIA DAN EFEK PROTEKTIF EKSTRAK DAUN PALIASA (KLEINHOVIAHOSPITA Linn) PADA SITOTOKSISITAS PANKREAS TIKUS HIPERGLIKEMIA) ........................... 411-417
ISWINARNO DOSO SAPUTRO, LOBREDIA ZARASADE, REVITA WIDYA PRASANTIHambatan Kolonisasi Methicillin Resistant Staphylococcus Aereus oleh EkstrakKulit Delima pada Luka Bakar Derajat-2 pada Tikus(INHIBITION OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS BACTERIACOLONIZATION ON SECOND DEGREE BURN WOUND IN WISTAR RATBY USING POMEGRANATE PEEL EXTRACT) ......................................................................................... 418-423
IWAN HARJONO UTAMA, SRI KAYATI WIDYASTUTI, ANAK AGUNG AYU MIRAH ADI,I GUSTI MADE KRISNA ERAWAN, IDA BAGUS OKA WINAYA, IDA BAGUS KOMANG ARDANA,ACHOIRO WATI RASID, TYAS PANDIEKA YOGAGambaran Sitologi Cairan Peritoneal dan Sinovial Itik Bali(CYTOLOGIC FIGURE OF PERITONEAL AND SYNOVIAL FLUID IN BALI DUCKS) ..................... 424-429
DILLA MAREISTIA FASSAH, LILIS KHOTIJAHPengimbuhan Vitamin-E dalam Ransum Kaya Asam Lemak Tidak JenuhTerhadap Profil Darah Induk Domba Laktasi§
(VITAMIN-E SUPPLEMENTATION ON RICH POLY-UNSATURATEDFATTY ACID RATION TO BLOOD PROFILE OF LACTATION EWES) ............................................... 430-439
HILMA PUTRI FIDYANDINI, MUNTI YUHANA, ANGELA MARIANA LUSIASTUTIPemberian Probiotik Multispesies dalam Media Budi Daya Ikan Lele Dumbo untukMencegah Penyakit Motile Aeromonads Septicemia(ADDITION OF MULTISPECIES PROBIOTICS IN THE CULTURE MEDIUM OF AFRICANCATFISH TO PREVENT THE MOTILE AEROMONADS SEPTICEMIA DISEASE) .......................... 440-448
ANINDYA KURNIAWATI, DENNY WIDAYA LUKMAN, I WAYAN TEGUH WIBAWANResistensi Antibiotik pada Salmonella Isolat Sapi Bakalan Asal Australia yang DiimporMelalui Pelabuhan Tanjung Priok Jakarta(ANTIBIOTIC RESISTANCE OF SALMONELLA ISOLATES FROM AUSTRALIAN IMPORTEDFEEDER CATTLES THROUGH TANJUNG PRIOK PORT JAKARTA) ................................................ 449-456
MUHAMMAD RIZA, MUHAMMAD RIYADHIFertilitas Semen Kerbau Rawa (Bubalus bubalis carabanensis) yang Diencerkandengan Pengencer Nira Aren(FERTILITY OF SWAMP BUFFALO SEMEN (BUBALUS BUBALIS CARABANENSIS)DILUTED WITH SUGAR PALM JUICE EXTENDER) ............................................................................... 457-467
DATIK SETIAWATI, BAMBANG SUKAMTO, HANNY INDRAT WAHYUNIPengimbuhan Enzim Fitase dalam Ransum Ayam Pedaging Meningkatkan PemanfaatanKalsium untuk Pertumbuhan Tulang dan Bobot Badan(ADDING PHYTASE ENZYMES ON BROILER RATION INCREASING CALCIUM UTILIZATIONFOR BONE GROWTH AND BODY WEIGHT GAIN) ................................................................................ 468-476
PEDOMAN BAGI PENULIS ................................................................................................................................... i-ii
Mitra Bebestari Maret 2015
Indrawati SendowBalai Besar Penelitian Veteriner (Balitvet) jln REMartadinata No 30, Bogor 16114
Prof Dr Siti Isrina Oktavia SalasiaLab Patologi Klinik Fakultas Kedokteran HewanUniversitas Gadjah Mada Jln Fauna No 2 KampusUGM Jogjakarta
Dr Rini WidayantiBagian Biokimia Fakultas Kedokteran HewanUniversitas Gajah Mada Jln Fauna No 2 Kampus UGMJogjakarta 55281
Dr Drh I Wayan Suardana MSiLab Kesehatan Masyarakat Veteriner FakultasKedokteran Hewan Universitas Udayana
Drh Cok Sari Nindhia MPLab Biostatistika Fakultas Kedokteran HewanUniversitas Udayana, Jln Sudirman Denpasar Bali
Prof Dr Drh Retno Damajanti MSLab Imunologi, Fakultas Kedokteran Hewan InstitutPertanian Bogor Jln Agatis Kampus IPB DramagaBogor 16680
Prof Wasmen Manalu PhDLab Fisiologi, Dept AFF, Fakultas Kedokteran HewanInstitut Pertanian Bogor, Jln Agatis Kampus IPBDramaga Bogor 16680
Dr Indrawati SendowBalai Besar Penelitian Veteriner (Balitvet) JLN REMartadinata No 30, Bogor 16114
Drh I Made Kardena MVScLab Patologi Fakultas Kedokteran Hewan UniversitasUdayana, Jln Sudirman Denpasar Bali
Dr Bayu RosadiFakultas Peternakan Universitas Jambi Jln JambiMuaro Bulian Km 15 Mandalo Darat Jambi
Prof Dr HerdisBadan Pengkajian dan Penerapan Teknologi (BPPT)Gedung II BPPT Lt.16 Jalan M.H. Thamrin No.8Jakarta Pusat 10340
Dr drh Arman SayutiLab Farmakologi, Fakultas Kedokteran HewanUniversitas Syiah Kuala, Jln Tengku Hasan KruengKalee No 4 Kampus Unsyiah, Darusalam Banda AcehNangroe Aceh Darusalam 23111
Prof Dr drh Tongku Nizwan Siregar MPBagian Reproduksi dan KebidananFakultas KedokteranHewan Universitas Syiah Kuala, Jln Tengku HasanKrueng Kalee No 4 Kampus Unsyiah, DarusalamBanda Aceh Nangroe Aceh Darusalam 23111
Prof drh Deni Noviana PhDBagian Bedah dan Radiologi, Departemen Klinik,Reproduksi dan Patologi, Fakultas Kedokteran Hewan,Institut Pertanian Bogor, Bogor, 16680, Tlp. (0251)8628080
MITRA BEBESTARI TAMU JURNAL VETERINER 2015 (1 dari 5)
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Dr Muhammad RizalKompleks Perumahan Meranti Griya Oxygen JlnOxygen 1 Blok D No 8 Kota Banjar Baru KalimantanSelatan 70711
Prof Dr drh Iman SupriatnaBagian Reproduksi dan KebidananDept Klinik Patologidan Reproduksi Fakultas Kedokteran Hewan, InstitutPertanian Bogor Kampus IPB Dramaga, Jln AgatisBogor 16680
Prof Drh Adji Santoso Dradjat MSc, PhDFakultas Peternakan, Universitas Mataram JlnMajapahit No 62 Mataram NTB
dr Yuliana M.BiomedLab Anatomi, Fakultas Kedokteran UniversitasUdayana, Jln Sudirman, Denpasar, Bali
Dr drh Gunanti, MSBagian Bedah dan Radiologi, Departemen Klinik,Reproduksi dan Patologi, Fakultas Kedokteran Hewan,Institut Pertanian Bogor, Bogor, 16680, Tlp. (0251)8628080
Prof drh Anak Agung Ayu Mirah Adi, MSi, PhDLab Patologi, Fakultas Kedokteran Hewan, UniversitasUdayana, Jln Sudirman Denpasar Bali
Dr drh Hera MaheswariLab Fisiologi, Dept AFF, Fakultas Kedokteran HewanInstitut Pertanian Bogor, Jln Agatis Kampus IPBDramaga Bogor 16680
Dr Agustina Dwi WijayantoBagian Farmakologi, Fakultas Kedokteran Hewan,Universitas Gadjah Mada, Kampus UGM, Jln FaunaNo 2 Jogjakarta 55281
Prof drh M Lazuardi, MSc, PhDBagian Farmasi, Fakultas Kedokteran HewanUniversitas Airlangga, Kampus-C Unair, JlnMulyorejo, Surabaya
Dr I Putu Sampurna MSLab Biostatistika Fakultas Kedokteran HewanUniversitas Udayana, Jln Sudirman Denpasar Bali
Dr Ir Bagus Priyo Purwanto, MAgrDepartemen Ilmu Produksi dan Teknologi PeternakanFakultas Peternakan, Institut Pertanian Bogor,KampusDramaga IPB, Jln Agathis, Bogor 16680
Ir Ari Sudewo MSFakultas Peternakan, Universitas Jendral Sudirman JlnSoeparno Karang Wangkal, Purwekerto 53123
Prof Dr Nono NgadionoFakultas Peternakan, Universitas Gadjah Mada, JlnFauna No 3, Kampus UGM, Jogjakarta, 55281
Prof Wasmen Manalu PhDLab Fisiologi, Dept AFF, Fakultas Kedokteran HewanInstitut Pertanian Bogor, Jln Agatis Kampus IPBDramaga Bogor 16680
Dr I Putu Sampurna MSLab Biostatistika Fakultas Kedokteran HewanUniversitas Udayana, Jln Sudirman Denpasar Bali
1
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Dr Theresa Nur Indah KoniJurusan Peternakan, Politeknik Pertanian NegeriKupang Jln Adi Sucipto, Penfui, Kupang, NusaTenggara Timur
Dr Mirni LamidDepartemen Peternakan, Fakultas Kedokteran HewanUniversitas Airlangga, Kampus-C Unair, JlnMulyorejo Surabaya
Prof I Made Mastika MSc, PhDFakultas Peternakan Universditas Udayana JlnSudirman Denpasar Bali
Prof Dr Cece SumantriLab. Genetika dan Pemulian Ternak, Departemen IlmuProduksi dan Teknologi Peternakan, FakultasPeternakan, Institut Pertanian Bogor, KampusDramaga IPB Jln Agatis Bogor 16680
Dr drh Wiwin Winarsih, MSiBagian Patologi, Fakultas Kedokteran Hewan InstitutPertanian Bogor, Kampus IPB Dramaga Jln AgatisBogor 16680
Dr drh Wisnu NurcahyoLab Parasitologi, Fakultas Kedokteran HewanUniversitas Gadjah Mada, Jln Fauna No 2, KampusUGM, Jogjakarta
Dr Drh I Nyoman Suartha MSiLab Penyakit Dalam Veteriner Fakultas KedokteranHewan Universitas Udayana
Prof drh Anak Agung Ayu Mirah Adi, MSi, PhDLab Patologi, Fakultas Kedokteran Hewan, UniversitasUdayana, Jln Sudirman Denpasar Bali
Prof drh Nyoman Suarsana, MSiLab Biokimia, Fakultas Kedokteran Hewan,Universitas Udayana, Jln Sudirman Denpasar Bali
Mitra Bebestari Juni 2015
Drh I Ketut Adnyane Mudite, PhDLab Histologi, Fakultas Kedokteran Hewan InstitutPertanian Bogor Jln Agatis Kampus IPB DramagaBogor 16680
Prof drh Anak Agung Ayu Mirah Adi, MSi, PhDLab Patologi, Fakultas Kedokteran Hewan, UniversitasUdayana, Jln Sudirman Denpasar Bali
Prof Dr Drh Fachriyan H PasaribuLab Mikrobiologi Medik, Fakultas KedokteranHewan, Institut Pertanian Bogor Jln Agathis KampusIPB Dramaga Bogor 16680
Dr Drh I Wayan Suardana MSiLab Kesehatan Masyarakat Veteriner FakultasKedokteran Hewan Universitas Udayana
Dr drh Maxs Urias Ebenhaizar Sanam MScFakultas Kedokteran Hewan, Univesitas Nusa CendanaJln Adi Sucipto, Penfui, Kupang, NTT 85001
Dr drh AAG PutraBalai Besar Veteriner Denpasar Jln Raya SesetanPegok, Denpasar
Dr Drh I Nyoman Suartha MSiLab Penyakit Dalam Veteriner Fakultas KedokteranHewan Universitas Udayana
Dr Inriyanti AssaFakultas Kesehatan Masyarakat UniversitasCendrawasih Jln Sentani Abepura Kampus BawahAbepura Jaya Pura, Papua
Dr Drh. Elok Budiretnani MS.Bag/Lab Helminthologi FKH IPB. Bidang Keahlian :Helminthologi
Prof Dr Putu SutisnaFakultas Kedokteran, Universitas Warmadewa JlnTerompong Denpasar Bali
Dr Drh. Elok Budiretnani MS.Bag/Lab Helminthologi FKH IPB. Bidang Keahlian :Helminthologi Fakultas Kedokteran Hewan, InstitutPertanian Bogor, Kampus IPB Dramaga
Dr Inriyanti AssaFakultas Kesehatan Masyarakat UniversitasCendrawasih Jln Sentani Abepura Kampus BawahAbepura Jaya Pura, Papua
Prof Dr Drh NS Dharmawan MSLab Patologi Klinik Veteriner Fakultas KedokteranHewan Universitas Udayana
Prof Dr Sri Subekti Bandryman SoedjokoLab Parasitologi, Fakultas Kedokteran HewanUniversitas Airlangga, Kampus-C Unair Jln Mulyorejo,Surabaya
Dr drh Angela Mariana Lusiastuti MSiInstalasi Litbang Pengendalian Penyakit Ikan JlnPerikanan No 13 Depok
Dr Woro Hastuti SatyantiniDept Manajemen Kesehatan Ikan dan BudidayaPerairan, Fakultas Perikanan dan Kelautan, UniversitasAirlangga, Kampus-C Unair, Jln Mulyorejo Surabaya
Dr Ni Nyoman Suryani MSiFakultas Peternakan, Universitas Udayana, JlnSudirman, Denpasar, Bali
Prof Dr Edy Rianto MScLab Produksi Ternak Potong dan Ternak Perah FakultasPeternakan dan Pertanian, Universitas Diponogoro JlnKampus drh Soejono Kusumowardoyo TembalangSemarang Jawa Tengah 50275
Prof Dr Asmuddin Natsir MScKompleks Universitas Hasanuddin (Unhas) Blok GBNo 28, Tamalanrea, Makassar 90245
Sri Sugiwati, S.Si, M.SiTaman Sari Bukit Damai Blok A4 No.18, GunungSindur Kab. Bogor Jawa Barat 16340
Dra Puspa Dewi N LotulungLab Kimia Terapan LIPI, Kompleks Puspitek Serpong,Tanggerang, Banten
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RALAT
Bersama ini kami sajikan ralat abstrak artikel atas nama Siti Gusti Ningrum et al; edisiMaret 2016. Abstrak bahasa Indonesia ini adalah pengganti abstrak bahasa Inggris yangditampilkan secara ganda pada penerbitan tersebut. Atas kekeliruan ini redaksi minta maaf.
ABSTRAK
Staphylococcus aureus adalah penyebab utama mastitis pada sapi perah. Pada penelitian ini,sebanyak 104 isolat Staphlylococcus yang berasal dari mastitis subclinik pada sapi dikarakterisasi secarafenotipe dan melacak keberadaan protein-A Staphylococcus (Spa). Resistensi sejumlah bakteri terhadapjuga dilakukan terhadap erythromycin, streptomycin, tetracycline, cefepime, nitrofurantoin, amikacin,chloramphenicol, dan ciprofloxacin. Sekitar 78% dari isolate tersebut bersifat sensitive sedang terhadapnitrofurantoin, sedangkan 89% sangat resisten terhadap cefepime dan ciprofloxacin. Dengan menggunakanberbagai sera mammalia, tujuh isolate terungkap menunjukkan memiliki Spa.
Kata-kata kunci: protein A, Staphylococcus aureus, mastitis subklinik, phenotyping
Kulit Dalem
CETAK LEPAS / REPRINTVol 17, No 3 September 2016
Terakreditasi Dirjen DiktiS.K. No. 81/DIKTI/Kep/2011
pISSN: 1411-8327; eISSN: 2477-5665Website : ojs.unud.ac.id
Terbit sejak 18 Desember 2000
Jurnal VeterinerJurnal Kedokteran Hewan Indonesia Indonesian Veterinary Journal
Naskah AsliOriginal Article
WYANDA ARNAFIA, SITI GUSTI NINGRUM, ERFIANDINI EKA PUSPITA,DENNY WIDAYA LUKMAN, FACHRIYAN HASMI PASARIBU, I WAYAN TEGUH WIBAWANCross Reaction of Serum in Salmonella enteritidis-Vaccinated Chicken to Some Salmonella enterica Serotypes(REAKSI SILANG SERUM AYAM YANG DIVAKSIN DENGAN SALMONELLA ENTERITIDIS TERHADAP BEBERAPA SEROTIPE SALMONELLA ENTERICA) ............................ 316-321
RISA INDRIANI, NI LUH PUTU INDI DHARMAYANTIPengembangan Vaksin Inaktif TeteloGenotipe VII Isolat Lokal pada Kondisi Laboratorium.(DEVELOPMENT OF TETELO INACTIVATED VACCINEGENOTYPE VII LOCAL ISOLATE IN LABORATORY CONDITION) ................................................... 322-330
ANDIKA BUDI KURNIANTO, GUSTI AYU YUNIATI KENCANA,I NYOMAN MANTIK ASTAWARespons Antibodi Sekunder Terhadap Penyakit Tetelo pada Ayam Petelur PascavaksinasiUlangan dengan Vaksin Tetelo Aktif(NEWCASTLE DISEASESECONDARY ANTIBODY RESPONSEAFTER REVACCINATION IN LAYER WITH THE ACTIVE ND VACCINE) ........................................ 331-336
WAHONO ESTHI PRASETYANINGTYAS, DENY PUTRA ROMADHON, FITRI SUSANA,ITA DJUWITA, KUSDIANTORO MOHAMADBlack Seed (Nigella sativa) Extract Induce in Vitro Proliferationand Differentiation of Rat Pancreatic and Bone Cells(EKSTRAK JINTAN HITAM (Nigela sativa) MENGINDUKSI PROLIFERASIDAN DIFERENSIASI SEL PANKREAS DAN SEL TULANG TIKUS SECARA IN VITRO) ................. 337-346
WORO HASTUTI SATYANTINI, AGUSTONO, ARIMBI,EMY KOESTANTI SABDONINGRUM, MYRNA BUDI, LINA WAFIA ASMIPeningkatan Respons Imun Non Spesifik Ikan Gurame PascapemberianEkstrak Air Panas Mikroalga Spirulina platensis(ENHANCEMENT OF NON-SPECIFIC IMMUNE RESPONSE OF OSPHRONEMUS GOURAMYAFTER GIVING OF HOT WATER EXTRACT SPIRULINA PLATENSIS) ............................................. 347-354
KHAIRUN NISAA, SUKENDA, MUHAMMAD ZAIRIN JUNIOR,ANGELA MARIANA LUSIASTUTI, SRI NURYATIBenih Keturunan Induk Ikan Nila yang Divaksinasi pada Tingkat Kematangan Gonad-2Lebih Tahan Terhadap Infeksi Streptococcus agalactiae(RESISTANCE OF FRY FROM TILAPIA (OREOCHRIMIS NILOTICUS) VACCINATIONBROODSTOCK AT DIFFERENT GONADAL DEVELOPMENTAL STAGESTO STREPTOCOCCUS AGALACTIAE INFECTION) ................................................................................ 355-364
NINING ARINI, MIRNAWATI SUDARWANTO, IDWAN SUDIRMAN, AGUSTIN INDRAWATIPemanfaatan Supernatan Lactobacillus plantarum Sebagai PenghambatPertumbuhan Escherichia coli pada Dangke Susu Sapi(UTILIZATION OF LACTOBACILLUS PLANTARUM SUPERNATANAS AN INHIBITIOR OF ECHERICHIA COLI GROWTH IN COW’S MILK DANGKE .......................... 365-373
KORBINIANUS FERIBERTUS RINCA, TJOKORDA SARI NINDHIA,I WAYAN SUARDANAFaktor-faktor Risiko Penyebaran Escherichia coli O157:H7pada Sapi Bali di Kuta Selatan, Badung, Bali(RISK FACTORS FOR DISSEMINATION OF ESCHERICHIA COLI O157:H7IN BALIN CATTLE IN SOUTH KUTA, BADUNG, BALI) ......................................................................... 374-382
TRI NURHAJATI, KOESNOTO SOEPRANIANONDO,WIDYA PARAMITA LOKAPIRNASARIUji Aktivitas Pertumbuhan Enterobacter cloacae Selulolitik Aerob Rumen-1 IsolatAsal Limbah Cairan Rumen Sapi Peranakan Ongole(GROWTH ACTIVITY ASSAY OF CELLULOLYTIC BACTERIA ENTEROBACTER CLOACAESAR 1 (CELLULOLYTIC AEROB RUMEN 1) ISOLATED FROM ONGOLE CROSSBREEDBOVINE RUMEN FLUID WASTE) ................................................................................................................. 383-388
I GUSTI AYU DEWI RATNAYANTI, NI PUTU SRIWIDYANI, I DEWA AYU INTEN PRIMAYANTI,I GUSTI KAMASAN, NYOMAN ARIJANA, I GUSTI NYOMAN SRI WIRYAWAN,IDA AYU IKA WAHYUNIARI, I WAYAN SUGIRITAMA, I GUSTI NGURAH MAYUNGrowth Hormone Menurunkan Ekspresi Protein p53 dan p21 Sel Endotel Tikus Jantan(GROWTH HORMONE REDUCES P53 AND P21 ENDOTHELIALPROTEIN EXPRESSION IN MALE RAT) .................................................................................................... 389-395
Vol 17, No 3 September 2016
Terakreditasi Dirjen Dikti
S.K. No. 81/DIKTI/Kep/2011
pISSN: 1411-8327; eISSN: 2477-5665Website : ojs.unud.ac.id
Terbit sejak 18 Desember 2000
Jurnal VeterinerJurnal Kedokteran Hewan Indonesia Indonesian Veterinary Journal
CETAK LEPAS / REPRINT
NOVI SUPRIHATIN, LIGAYA ITA TUMBELAKA, MOHAMAD AGUS SETIADIProfil Progesteron Air Susu dan Tingkat Kebuntingan Sapi Perah PascasinkronisasiEstrus Menggunakan Prostaglandin F
2Alfa atau Progesteron-CIDR
(MILK PROGESTERONE PROFILE AND PREGNANCY RATE ON DAIRY CATTLEAFTER ESTROUS SYNCHRONIZATION WITH PROSTAGLANDIN F
2
ALFA OR PROGESTERONE-CIDR) .............................................................................................................. 396-403
RINI WIDYASTUTI, SONY HERU SUMARSONO, ARIEF BOEDIONO, SITI DARODJAH RASADLow Concentration of Ethylene Glycol Improve Recovery Rate of HumanSpermatozoa After Vitrification(ETILEN GLIKOL KONSENTRASI RENDAH MENINGKATKANRECOVERY RATE SPERMATOZOA MANUSIA PASCAVITRIFIKASI) ................................................. 404-410
YULIANA, SIANNY HERAWATIPhytochemical Content and Protective Effect of Kleinhovia hospital Leaves Extracton Pancreatic Cytotoxicity in Hyperglycemic Rats(KANDUNGAN FITOKIMIA DAN EFEK PROTEKTIF EKSTRAK DAUN PALIASA (KLEINHOVIAHOSPITA Linn) PADA SITOTOKSISITAS PANKREAS TIKUS HIPERGLIKEMIA) ........................... 411-417
ISWINARNO DOSO SAPUTRO, LOBREDIA ZARASADE, REVITA WIDYA PRASANTIHambatan Kolonisasi Methicillin Resistant Staphylococcus Aereus oleh EkstrakKulit Delima pada Luka Bakar Derajat-2 pada Tikus(INHIBITION OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS BACTERIACOLONIZATION ON SECOND DEGREE BURN WOUND IN WISTAR RATBY USING POMEGRANATE PEEL EXTRACT) ......................................................................................... 418-423
IWAN HARJONO UTAMA, SRI KAYATI WIDYASTUTI, ANAK AGUNG AYU MIRAH ADI,I GUSTI MADE KRISNA ERAWAN, IDA BAGUS OKA WINAYA, IDA BAGUS KOMANG ARDANA,ACHOIRO WATI RASID, TYAS PANDIEKA YOGAGambaran Sitologi Cairan Peritoneal dan Sinovial Itik Bali(CYTOLOGIC FIGURE OF PERITONEAL AND SYNOVIAL FLUID IN BALI DUCKS) ..................... 424-429
DILLA MAREISTIA FASSAH, LILIS KHOTIJAHPengimbuhan Vitamin-E dalam Ransum Kaya Asam Lemak Tidak JenuhTerhadap Profil Darah Induk Domba Laktasi§
(VITAMIN-E SUPPLEMENTATION ON RICH POLY-UNSATURATEDFATTY ACID RATION TO BLOOD PROFILE OF LACTATION EWES) ............................................... 430-439
HILMA PUTRI FIDYANDINI, MUNTI YUHANA, ANGELA MARIANA LUSIASTUTIPemberian Probiotik Multispesies dalam Media Budi Daya Ikan Lele Dumbo untukMencegah Penyakit Motile Aeromonads Septicemia(ADDITION OF MULTISPECIES PROBIOTICS IN THE CULTURE MEDIUM OF AFRICANCATFISH TO PREVENT THE MOTILE AEROMONADS SEPTICEMIA DISEASE) .......................... 440-448
ANINDYA KURNIAWATI, DENNY WIDAYA LUKMAN, I WAYAN TEGUH WIBAWANResistensi Antibiotik pada Salmonella Isolat Sapi Bakalan Asal Australia yang DiimporMelalui Pelabuhan Tanjung Priok Jakarta(ANTIBIOTIC RESISTANCE OF SALMONELLA ISOLATES FROM AUSTRALIAN IMPORTEDFEEDER CATTLES THROUGH TANJUNG PRIOK PORT JAKARTA) ................................................ 449-456
MUHAMMAD RIZA, MUHAMMAD RIYADHIFertilitas Semen Kerbau Rawa (Bubalus bubalis carabanensis) yang Diencerkandengan Pengencer Nira Aren(FERTILITY OF SWAMP BUFFALO SEMEN (BUBALUS BUBALIS CARABANENSIS)DILUTED WITH SUGAR PALM JUICE EXTENDER) ............................................................................... 457-467
DATIK SETIAWATI, BAMBANG SUKAMTO, HANNY INDRAT WAHYUNIPengimbuhan Enzim Fitase dalam Ransum Ayam Pedaging Meningkatkan PemanfaatanKalsium untuk Pertumbuhan Tulang dan Bobot Badan(ADDING PHYTASE ENZYMES ON BROILER RATION INCREASING CALCIUM UTILIZATIONFOR BONE GROWTH AND BODY WEIGHT GAIN) ................................................................................ 468-476
PEDOMAN BAGI PENULIS ................................................................................................................................... i-ii
411
Phytochemical Content and Protective Effect
of Kleinhovia hospital Leaves Extract
on Pancreatic Cytotoxicity in Hyperglycemic Rats
(KANDUNGAN FITOKIMIA DAN EFEK PROTEKTIF
EKSTRAK DAUN PALIASA (KLEINHOVIA HOSPITA Linn)
PADA SITOTOKSISITAS PANKREAS TIKUS HIPERGLIKEMIA)
Yuliana1, Sianny Herawati2
1Department of Anatomy, 2Department of Clinical Pathology,
Faculty of Medicine, University of Udayana,
Jln. Sudirman, Denpasar, Bali, Indonesia
Phone: 0361 222510; Email: [email protected]
ABSTRACT
Diabetes mellitus is a wide problem nowadays. Many traditional plants are used to overcome thecomplication of this disease. One of them is paliasa (Kleinhovia hospital Linn) leaves. This study aimswere to investigate phytochemical contents and the protective effects of paliasa leaves extract on pancreaticcytotoxicitybased on microscopic lesion such as hemorrhagic score and necrotic appearances, in alloxan-induced hyperglycemic rats. Male Wistar rats (three months old) were divided into four groups consistedofseven rats each. Group I was diabetic control, Group II was diabetic groups, paliasa extract (300mg/kgBW) were given by sonde for a period of 14 days prior to alloxan injection (150 mg/kg intraperitoneal).Group III was diabetic rats given 600 mg/kg BW paliasa extract and group IV was diabetic rats given 900mg for 14 days. At the end of the study, rats were sacrificed. Tissue sample ofpancreaswas then processedfor slide preparation and was stain with hematoxylin eosin.Pancreas hemorrhagic score was divided intofour criteria, focal(score 1), multifocal (score 2), extensive (score 3), and difuse (most severe, score 4).Microscopic examination was done using binocular microscope, at Laboratory ofVeterinaryPathology,Disease Investigation Centre Denpasar, Bali. Data werethenanalyzed by using analysis ofvarians. Study showed that paliasa extract could lower hemorrhagic score on pancreas of diabetic rats,even though it was not significant compared to control group (p 0,205). Phytochemical analysis showedthat paliasa leaves extract contained alkaloid, terpenoid, and flavonoid. Necrotic appearances were variedfrom pycnosis, karyorheksis, karyolysis, and vacuolization. In conclusion, paliasa leave extract may haveprotective effect on pancreas cytotoxicity.
Key words: alloxan; paliasa extract; diabetes; phytochemical; rats
ABSTRAK
Diabetes mellitus merupakan masalah yang sering dijumpai di dunia. Banyak tanaman tradisionaldigunakan untuk mengatasi komplikasi penyakit ini. Salah satu di antaranya adalah daun paliasa(Kleinhovia hospital). Penelitian ini bertujuan untuk mengetahui kandungan fitokimia dan efek proteksiekstrak daun paliasa terhadap sitotoksisitas pancreas (tingkat perdarahan dan gambaran nekrosis)pada tikus Wistar hiperglikemia yang diinduksi aloksan. Penelitian menggunakan rancangan ControlGroup Design, menggunakan 28 ekor tikus berumur tiga bulan. Tikus-tikus percobaan dibagi secara acakmenjadi empat kelompok, yaitu kelompok kontrol, paliasa dosis 300mg/kg berat badan (BB), 600 mg/kgBB, dan 900 mg/kg BB. Setelah diberikan perlakuan selama 14 hari, tikus dikorbankan nyawanya dandilakukan pemeriksaan pankreas dengan pengecatan hematoxylin eosin. Tingkat perdarahan pankreasdibagi menjadi empat skor lokal (skor 1), multifokal (skor 2), ekstensif (skor 3), dan difus (paling berat,diberi skor 4). Pemeriksaan mikroskopis memakai mikroskop cahaya binokuler. Pemeriksaan dilakukandi Laboratorium Patologi Veteriner, Balai Besar Veteriner (BB Vet) Denpasar, di Pegok, Denpasar, Bali.Analisis data dilakukan dengan sidik ragam. Penelitian menunjukkan bahwa ekstrak paliasa dapatmenurunkan tingkat perdarahan pancreas tikus hiperglikemik, namun tidak signifikan (p 0,205). Ujifitokimia dilakukan di Jurusan Farmasi Fakultas MIPA Universitas Udayana. Hasil penelitianmenunjukkan uji fitokimia ekstrak tanaman paliasa mengandung alkaloid, terpenoid, dan flavonoid.Gambaran nekrosis bervariasi dari piknosis, karioreksis, kariolisis, dan vakuolisasi. Ekstrak tanamanpaliasa memiliki efek protektif pada sitotoksisitas pancreas.
Kata-kata kunci: aloksan; ekstrak paliasa; diabetes; fitokimia; tikus
Jurnal Veteriner September 2016 Vol. 17 No. 3 : 411-417pISSN: 1411-8327; eISSN: 2477-5665 DOI: 10.19087/jveteriner.2016.17.3.411Terakreditasi Nasional SK. No. 15/XI/Dirjen Dikti/2011 online pada http://ojs.unud.ac.id/php.index/jvet
412
INTRODUCTION
Diabetes mellitus is a worldwide disease and
is depicted in blood glucose increasing above
normal. World Health Organization predicted
that more than 220 million people in the world
will suffer from diabetes and this number will
be doubled in 2030 (Hamid and Moustafa, 2013).
In Indonesia, it is estimated that 50% of diabetic
patients had not been diagnosed (Muhtadi et al.,
2015).
Oral anti-diabetic and insulin sensitizer can
repair insulin insensitivity, increase insulin
production, and lower blood glucose. However,
those medicines have some side effects, such as
hypoglycemia at higher dosage, low oral
bioavailability, and low permeability when
entering intestine epithelium. This will urge
increasing demand of anti diabetic herbal
products with relatively fewer side effects
(Malekpour et al., 2012). One of traditional
herbal plants that used to treat diabetes is
paliasa. Paliasa (Kleinhovia hospital Linn) is
found at South Sulawesi. Paliasa leaves can be
used as vegetables, supportive medicine for liver
disease, and combat hair flea (Dini, 2009).
Diabetes mellitus induced by alloxan is type-
1 diabetes mellitus, insulin dependent diabetes
mellitus (Viana et al., 2004). It happened due to
autoimun disorder and beta cell pancreas
damage in Langerhans islets. First, beta cell
pancreas decrease in number and volume, then
insulin deficiency will happen permanently
(Waer and Helmy, 2012). Diabetes mellitus
treatment without any side effects is a big
challenge for future. WHO declared that anti-
diabetic herbal medicine need special concerns
(Patil et al., 2011).
Hiperglycemia stimulates reactive oxygen
species from many ways such as oxidative
fosforilation, glucose autooxidation, lipooxy-
genase, and nitrit oxide sintase. Natural anti
oxidant in our body is not sufficient to neutralize
free radicals that can cause tissue damage. Thus,
herbal anti oxidant is needed as an alternative
for the treatment (Hamzah et al., 2012).
In diabetic rats, pancreas cells showed
granular degeneration, pycnosis, karyorrhexis,
and karyolysis (Liu et al., 2014). In histo-
pathology of alloxan induced diabetic rats, Mir
et al. (2013) found changes in exocrine and
pancreatic beta cells. On the islets of Langerhans
cell degranulation occurs, accompanied by the
formation of ghost islets with loss of whole cells.
Bleeding is also found in all parts of the gland.
This study aims were to investigate phyto-
chemical contents and the protective effects of
paliasa leaves extract on pancreatic cytotoxicity
(hemorrhagic score and necrotic appearances) in
alloxan-induced hyperglycemic rats.
RESEARCH METHODS
Plant Material Collection and Preparation
Paliasa leaves were harvested from
Makassar, a city of South Sulawesi Province.
The process of extraction was done as following:
1 kg of dry paliasa leaves, were poured with 15
litres methanol in room temperature (26°C) for
14 hours, then they were filtered with Whatman
paper. Filtrate was dried by vacuum through
rotary evaporation (Arung et al., 2009).
Animals
Three month-old male Wistar rats (180-200
g body weight) bred in Pharmacology Depart-
ment, Faculty of Medicine, University of
Udayana, animal house were used. The rats were
housed in cages with room temperature (26°C).
This experimental protocol was approved by
Ethics Committee of Animal Usage for Research
and Education from Faculty of Veterinary
Medicine, Udayana University (Letter Number:
191/KE-PH-Lit-2/V/2015 on 12 May 2015).
Alloxan was purchased from Pharmacological
Laboratory of Widya Mandala University,
Surabaya, Indonesia.
Experimental Design
A total of 28 rats were randomly divided into
four groups of seven each and induced with
alloxan 120 mg/kg BW intraperitoneal in saline.
All rats were found to be diabetic after 72 h. Rats
with a blood glucose level above 150 mg/ dL were
considered to be diabetic (Agunbiade et al., 2015).
Group I served as the diabetic control; Group II
were given paliasa leaves extract by using sonde
orally with dose 300 mg/kg BW; Group III were
given paliasa leaves extract by using sonde orally
with dose 600 mg/kg BW; Group IV were given
paliasa leaves extract by using sonde orally with
dose 900 mg/kg BW. The curative effect of paliasa
extract was then evaluated for a period of 14 days
after alloxan administration and animals were
sacrificed by using chloroform. Pancreases were
collected immediately for histopathological
examination.
Yuliana, et al Jurnal Veteriner
413
Glycoside. It is done by Liebermann-
Burchard reaction. One mililiter extract was
evaporated; the rest was soluted in 5 mL
anhidrate acetat acid, and then added with 10
drop sulfate acid. The extract has black brown
colour, it meant negative for glycoside.
Flavonoid. One mililiter extract solution
was evaporated, the rest was wetted by aseton,
enhanced by a little of smooth borac acid powder
and oxalic acid, then it was heated carefully. After
that, the rest was mixed with 10 mL eter. By
using UV366
; the extract showed intensive
yellowish fluorescence, and it meant there was
flavonoid. Phytochemical test showed that
paliasa contains alkaloid, terpenoid, and
flavonoid
Statistical Analysis.
All the grouped data were statistically
evaluated with one way analysis of variance
(Anova), P< 0.05 was considered statistically
significant.
RESULTS AND DISCUSSION
Result of treatment effect was shown on
Table 1 (electric microscope, 400x)
The Table 1 showed the mean pancreatic
hemorrhagic score between groups after being
given treatment did not differ significantly (p>
0.05). Necrosis appearances were varies, from
pycnosis, karyolysis, and karyorhexis as shown
in Figure 1.
Alloxan is beta cytotoxin. Alloxan chemically
induces diabetes by destroying beta cells that
produce insulin. A decrease in the release of
insulin lowers insulin usage to neutralize blood
glucose in the body. Increased oxygen free
radicals occur due to high blood glucose levels.
Histopathological Studies
Pancreatic tissues from all groups were
subjected to histopathological studies and
collected in jar contained with approximately
10% buffered formalin solution. Sections of
approximately 5 µm thickness of the pancreas
were made and stained with hematoxylin and
eosin (HE tissue staining) and they were
examined for pancreatic hemorrhagic score.
Stained sections were qualitatively evaluated
using an electric binocular microscope (Olympus
Japan), with magnification 400 times.
Pancreas hemorrhagic score was divided into
four groups, i.e. local (score 1), multifocal (score
2), extensive (score 3), and diffuse (score 4).
Phytochemical Analysis
Phytochemical analysis was done at
Pharmacy Department, of the Faculty of
Mathematics and Natural Science, Udayana
University, in order to investigate the main
content of paliasa extract that has effect in
protecting pancreas.
Atsiri Oil
Extract was added with etanol. Paliasa
leaves extract has no aromatic smell although
it was evaporated until dry.
Alkaloid. Two mililiters extract was
steamed until it get residu. Residu was added
with 5 mL HCl 2N, then it was divided to five
reaction tube. First tube was added with weak
acid that acted as blanko. Second tube was added
with three drops Dragendorrff, third tube was
added with three drops Mayer reaction, fourth
tube was added wagner reaction. The paliasa
extract showed orange sediment in second tube
and white yellowish sediment in third tube. It
showed there was alkaloid.
Sterol and terpenoid. One mililiter
extract was steamed, and then it was added by
0.5 mL chloroform, and added by 0.5 mL anhidrat
acetic acid. Then it was dropped by 12 mL
sulphate acid. The extract showed brownish or
violet ring in the boundary, it revealed there was
terpenoid.
Saponin. Three mililiters paliasa extract
was shaken vertically for 10 seconds, then it
waited for 10 seconds. The extract had no stable
foam, it meant there was no saponin.
Polyphenol. One mililiter extract was
added with iron/ferri (III) chloride 10%, if it
turned to dark blue, blue black or black green,
then it meant there was polyphenol there. The
extract showed negative for polyphenol.
Table 1. Mean pancreatic hemorrhagic score of
diabetic rats after treatment with
paliasa extract (n=7).
Treatment Group Mean pancreatic
hemorrhagic
score ± SD
Control 3,14 ± 0,690
Paliasa 300 2,71 ± 0,756
Paliasa 600 2,57 ± 0,535
Paliasa 900 2,43 ± 0,545
Jurnal Veteriner September 2016 Vol. 17 No. 3 : 407-413
414
angiotensin system locally on the pancreas will
increase pancreatic damage induced by ROS
(Leung, 2007).
Beta cells of diabetic rats showed
vacuolization and a decrease in secretory
granules, fusion of some granules and pycnotic
nuclei (Hamid and Moustafa, 2013). Giving
alloxan causes morphological changes in diabetic
rats in the form of severe damage to the beta
cells of the pancreas, which is characterized by
a decrease in the number of cells, cell damage,
and even cell death (Dahecha et al., 2011).
Necrosis is usually found in the middle of
Langerhans islet because this part is initially
influenced by alloxan (Singh and Gupta, 2007).
Karyolysis, the loss of the cell nucleus, as well
as the presence of remnants (residue) cells that
have vacuole shaped found in research Jelodar
et al. (2005). At the core of beta cells that undergo
karyolisis, also showed debris in the form of the
Auto oxidation produces free radicals and this is
exacerbated by the effects of diabetogenic alloxan
(Hamzah et al., 2012).
Alloxan and its reduction product, dialuric
acid, cause a redox cycle through the formation
of superoxide radicals. Superoxide radicals
experience dismutase releases hydrogen peroxide.
Highly reactive hydroxyl radicals are formed by
Fenton reaction (Rohilla and Adi, 2012).
The effects of reactive oxygen species (ROS)
along with an increase in cytosolic calcium
concentration would lead to massive destruction
of beta cells rapidly (Hamid and Moustafa, 2013).
The severity of tissue damage and the degree of
oxidative stress may depend on an imbalance
between excessive ROS production and
antioxidant defenses in the cells of the pancreas
(Kikumoto et al., 2010). Reactive oxygen species
are involved in various signaling pathways of
angiotensin II. Increased activity of the renin-
Figure 1. Necrosis appearance in pancreas of four study group (HE, 400x). Figure A is pancreas
of hyperglycemic (arrow showed karyorhexis). Figure B is pancreas of hyperglycemic
rats with 300 mg/kg BW paliasa extract (there is pycnosis, karyorhexis, and karyolysis).
Figure C is pancreas of hyperglycemic rats with 600 mg/kg BW paliasa extract (there is
karyorhexis). Figure D is pancreas of hyperglycemic rats with 900 mg/kg BW paliasa
extract (there is pycnosis and vacuola).
Yuliana, et al Jurnal Veteriner
415
fragment mass surrounding the cell nucleus
(Boudreau et al., 2006).
Pancreas is more susceptible to oxidative
stress compared to other organs as the cells in
the pancreas have lower anti-oxidant enzymes
(Robertson, 2006). Oxidative stress that induced
by alloxan will cause breakdown of DNA and
activate polysintetase resulting in impaired
synthesis of insulin in the pancreas (Takemoto
et al., 2014). Pancreas functions as a glucose
sensor and insulin-requiring oxygen-rich
environment as well as glucose, so that it can
generate sufficient signal for insulin secretion
and providing adequate insulin to the target
tissues. These special circumstances make
pancreatic beta cells are highly susceptible to
oxidative stress (Lenzen, 2008). Hyperglycemic
conditions lead to an increase in the concen-
tration of H2O
2 and induce catalase activity as a
defense mechanism against free radicals.
However catalase levels may not be sufficient to
prevent the onset of diabetes (Kikumoto et al.,
2010).
Vacuolization is one indication of structural
disruption of membrane permeability, cause
increase in fluid and electrolyte transport into
the cell. Permeability disorders occur due to
reactive oxygen species (Hamid and Moustafa,
2013). Variations of histopathological pancreas
may occur because of differences in the status of
anti-oxidants, as well as the size and maturity
of cells (Mir et al., 2013).
Anti-oxidants contained in the plants may
act to neutralize free radicals. Free radicals are
associated with the development of degenerative
disorders such as diabetes mellitus and
cardiovascular related diseases (Renjith et al.,
2013). A phytochemical test result of paliasa
plant contains alkaloids, terpenoids and
flavonoids. Alkaloids may act to stimulate the
multiplication of existing cells in Langerhans
islets and differentiation into new cells. Alkaloids
maybe also important for the recovery of partial
beta cell (Singh and Gupta, 2007). Alkaloid has
anti-hyperglycemic, anti-inflammatory, and
anti-oxidants effects (Adeneye and Crook, 2015).
Alkaloids lower insulin-mediated glucose disposal
and reduce activities of two enzymes that are
essential for the production of glucose, namely
glycogen phosphorylase and glucose-6-
phosphatase (Ezuruike and Prieto, 2014).
Terpenoids and flavonoids have anti-diabetic
properties. Flavonoids are able to regenerate the
damaged beta cells in alloxan-induced diabetic
rats and act as insulin secretagogous
(Balamurugan et al., 2014). Liu et al. (2007)
found that flavonoids can enhance insulin
sensitivity. Terpenoids and flavonoids may be
able to improve the integrity of the B-
endocrinocyte through increasing the release of
insulin or insulin activity improvement (Snigur
et al., 2008).
Flavonoids can modulate the activity of
glycolytic and gluconeogenic enzymes (Renjith
and Rajamohan, 2012). Flavonoids inhibit the
glucose transporter epithelial cells in the small
intestine and regenerate the damaged beta cells
in the alloxan induced of pancreatic beta cells
(Sharma et al., 2013). The formation of advanced
glycated end products (AGEs) can be prevented,
and there is no atherosclerosis, nephropathy, and
retinopathy (Rahimi et al., 2005).
An efficacy hypoglycemic herb are obtained
through a process of increasing insulin secretion,
inhibit glucose absorption from the intestine,
inhibits glucose production from hepatocytes,
and increase glucose uptake by muscle and fat
(Hui et al., 2009). Giving phytonutrients may
be an effective strategy to overcome the
complications of diabetes by regulating the
enzymes that responsible for metabolizing
glucose (Renjith and Rajamohan, 2012). The
active substance in traditional crops can activate
the regeneration of pancreatic beta cells (Jelodar
et al., 2005). Pancreatic function recovery occurs
through the facilitation of metabolites in insulin-
dependent process (Balamurugan, 2014).
CONCLUSION
The conclusion obtained from the study is:
paliasa plant extracts contain alkaloids,
terpenoids and flavonoids, and paliasa plant
extract given to hyperglycemic rats may not
signifcantly reduce the level of hemorrhagic
pancreas.
SUGGESTION
Further studies are needed to investigate
each phytochemical content of paliasa extract
towards pancreas chemical markers.
ACKNOWLEDGEMENT
The authors are grateful to Udayana Uni-
versity for the financial assistance, with
Jurnal Veteriner September 2016 Vol. 17 No. 3 : 407-413
416
Contract Letter number 1438/UN14.2/PNL/
01.03.00/2015 (Donation of Study Program).
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