大阪府立大学大学院生命環境科学研究科学術報告

　Vol. 59 2008　

ISSN 1881-6789

Scientific Report

of

the Graduate School of Life and Environmental Sciences,

Osaka Prefecture University

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Prefecture University is official online journal of the Graduate School of Life and Environmental Sciences, Osaka Prefecture University concerning to the disciplines in life and environmental sciences. One volume is annually uploading on Graduate School Library Home Page, containing articles (original and short research papers), reviews (doctor dissertation), notes and communication which were pier reviewed by anonymous referees or editorial board. One of the authors should be the professors or instructors of the Graduate School of Life and Environmental Sciences, University of Osaka Prefecture.　

Note: The Scientific Report of the Graduate School of Life and Environmental Sciences, Osaka

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Cover illustration: Millet strage at Mali, Africa (Photograph: N68-C14-11 in 1968 by Dr. S. Nakao)

Editorial Board

Professor Hirofumi YAMAGUCHI (Plant BioScience)Professor Naoya KASAI (Applied Biochemistry)Professor Mitunori KIRIHATA (Bioinformatics)Professor Hisayuki MAENAKA (Environmental Sciences and Technology) Professor Yoichi NAKAMURA (Veterinary Science)

Editor-in-ChiefEditors

Original Paper／原著論文

Tarumoto, I., E. Ishii, M. Yanase and M. Fujimori

　The Phenotypic Fluctuation Factor for Male Sterility in A1

　Male Sterile Lines of Sorghum (Sorghum bicolor Moench)　 ………………………………　１

Notes／資料

道下雄大・梅本信也・山口裕文：

　西南日本の民家庭園における維管束植物の追加・修正 …………………………………………　７

Instructions for Authors／執筆要領　 ……………………………………………………………　�

Contents／目次

ISSN 1881-6789

Introduction

　Cytoplasmic male sterility (CMS) is currently desired for the production of F1 hybrid sorghum (Sorghum bicolor Moench) seed, since manual emasculation is impractical (Stephens and Hol-land, 1954). Among several sources of CMS in sorghum, A1 cytoplasm is the major male-sterile cytoplasm derived from ‘Milo’ (Pring et al., 1982; Pring et al., 1995; Xu et al., 1995). The male-ster-ile lines (A-lines) with A1 cytoplasm are the most important lines for production of F1 hybrid seeds in Japan as well as in the USA (Schertz and Ritchey, 1978; Tarumoto, 1971). However, the A-lines that ensured their sterility at Nishi-nasuno (National Grassland Research Institute, Tochigi, Japan; latitude 36°55’ N; Daily mean temperature (DMT) of July 21.8℃) sometimes

showed unstable male sterility at Higashi-Hiroshima (Hiroshima Pref. Agriculture Research Center, Hiroshima, Japan; latitude 34°25’ N; DMT of July 24.8℃). A similar fluctuation of their male sterility was observed at Sakai (Osaka Prefecture University, Osaka, Japan; lati-tude 34°35’ N; DMT of July 26.4℃), assuming that the temperature stimulus in the duration of flower development causes phenotypic fluctua-tion for sterility in A1 male sterile lines. The con-version from male fertility to male sterility, due to a low night temperature, was known in sev-eral sorghum genotypes (Brooking, 1979). In rice, thermo-sensitive genetic male-sterile lines were derived by conversion from male fertility to male sterility either by daily mean tempera-ture above 24-26℃ (Latha et al., 2004) or by

－１－

Sci. Rep. Grad. Sch. Life. & Envi. Sci. Osaka Pref. Univ. 59:1-6 (2008) Original

Abstract

　The present study was conducted to clarify the factors shaping the extent of male sterility in A1 male-sterile lines of sorghum (Sorgum bicolor Moench). Eleven male-sterile lines (A-line) with A1 cytoplasm were grown both on a university farm during crop season and in growth chambers controlled under three different temperatures during the period from the 3rd to the 10th leaf stages in 1999. Based on the degree of seed and pollen fertility in artificial self-polli-nated plant, A-lines were classified into 4 groups. Six cultivars of Group-1, ‘MS79,’ ‘CK60,’ ‘Martin,’ ‘Reliance,’ ‘Wheatland’ and ‘Tx624,’ showed stable male-sterility in all experimental conditions examined. ‘MS138’ of Group-2 showed variable sterility under minimum tempera-tures above 24℃, and ‘Tx3048’ of Group-3 showed variable sterility under maximum tempera-tures above 26.5℃ at flower initiation stage. Three cultivars of Group-4, ‘Redbine58,’ ‘Redlan’ and ‘Westland,’ showed variable sterility with restoration of pollen fertility in the field condition. In male-sterile sorghum lines like ‘MS138’ and ‘Tx3048,’ a physiological condition between early vacuolated pollen stage and engorged pollen stage is considered to be critical for conversion from sterile to fertile.

Key Words: A1 cytoplasm, Cytoplasmic male sterility, Sorghum, Temperature.

The Phenotypic Fluctuation Factor for Male Sterility in A1 Male-Sterile Lines of Sorghum (Sorgum bicolor Moench)

Isao TARUMOTO1*, Emi ISHII (ADACHI)1, 3, Masanori YANASE1 and Masahiro FUJIMORI2, 4

(1 Laboratory of Genetics and Plant Breeding, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, Sakai 599-8231, Japan.; 2 National Institute of Livestock and Grassland Sciences, Nasushiobarashi 329-2793, Japan; 3 Shinwa Junior and Girls' Senior High School, Kobe 657-0022, Japan;

4 Yamanashi Prefectural Dairy Farm Experiment Station, Nagasaka 408-0021, Japan)*Corresponding to e-mail: i_taru2006@ybb.ne.jp

maximum temperature higher than 30℃ (Lopez et al., 2003). However, the conversion from male sterility to male fertility has not been known in sorghum varieties except for in these cytoplas-mic male-sterile lines (Adachi, 2000; Tarumoto and Oizumi, 1967; Tarumoto et al., 2000). There-fore, in order to clarify the factors of fluctuation of male-sterility, the seed and pollen fertility of A-lines with A1 cytoplasm was examined for sor-ghum genotypes grown in an experimental field and in growth chambers under different tem-peratures on the campus of Osaka Pref. Univer-sity in 1999.

Materials and Methods

Eleven A-lines with A1 cytoplasm (Table 1) whose cytoplasmic type was determined as A1 (Adachi 2000) for study on the stability of male sterility and eleven counterpart maintenance lines (B-line) as a control were used in this study. These lines were grown on a university farm (latitude 34°33’ N) during the 1999 crop-ping season from June 10 to September 20. The field condition (FC) was 29.3℃ average maxi-mum temperature, 21.9℃ average minimum temperature and 25.6℃ daily mean tempera-ture (DMT) during a period from the 3rd to the

10th leaf stages (June 23 to July 28) and flower development duration. Eleven A-lines were grown in three growth chambers controlled under different day/night temperatures (Table 2) during a period from the 3rd to the 10th leaf stages although they were maintained outdoors before and after temperature treatment. The growth chambers were maintained at 24℃/15℃ (day/night) as a low temperature condition (LTC, 19.7℃ DMT) at 24℃/24℃ as a medium temperature condition (MTC, 24℃ DMT) and

－２－

I. Tarumoto et al.:

Table 1. Male-sterile lines (A-line) with A1

cytoplasm and their origin.

Origin and Organization introducedA-lines

IS2830A&B, Purdue Univ., USA MS79

Combine kafir 60A&B, Texas A&M Univ., USACK60

MartinA&B, Iowa State Univ., USAMartin

RelianceA&B, Iowa State Univ., USAReliance

Wheatland A&B, Iowa State Univ., USAWheatland

Tx624A&B, Texas A&M Univ., USATx624

932233A&B, Purdue Univ., USAMS138

Tx3048A&B, Texas A&M Univ., USA Tx3048

Redbine58 A&B, Iowa State Univ., USARedbine58

Redlan A&B, Texas A&M Univ., USARedlan

Westland A&B, Texas A&M Univ., USAWestland

Fig. 1. The pollen observed at early vacuolated microspore stage (a), vacuolated pollen stage (b)

and engorged pollen stage (c). The pollen reached at engorged stage (c) is classified into

fertile.

at 33℃/24℃ as a high temperature condition (HTC, 28.7 DMT) to reveal seed and pollen fertility. The panicles of A-lines in all experi-ments and those of B-lines in FC were covered by paper bags before flowering and debagged three weeks after flowering, and then self-polli-nated panicles were harvested after ripening. The percentage of seed fertility was determined for at least 200 spikelets per replicate. The pol-len was collected from anthers one day before dehiscent and was stained with 0.5 % aceto-car-mine solution after fixation by 70% ethanol. The pollen which achieved engorged pollen stage (Christensen et al. 1972) was considered to be fertile. The other pollen before early vacuolated microspore stage or vacuolated pollen stage was treated as sterile (Fig. 1). The percentage of pol-len fertility was determined by the evaluation of at least 100 pollens per replication.

Results and Discussion

Seed fertility and pollen fertility of A-lines in four experimental conditions and B-lines in FC are shown in Table 3. The percentages of seed and pollen fertility in B-lines were 65 to 93% and 54 to 99%, respectively, which indicates that B-lines expressed their normal fertility as maintenance lines. No seed was set in A-lines, ‘MS79,’ ‘CK60,’ ‘Martin,’ ‘Reliance,’ ‘Wheatland’ and ‘Tx624’ by artificial self-pollination under all four conditions. However, ‘MS138’ showed 19% seed set in MTC (day/night, 24℃/24℃) and 21% seed set in HTC ( 33℃/24℃), ‘Tx3048’ showed 15% seed set in HTC and 13% seed set in FC (29.3℃/21.9℃), and ‘Redbine58,’ ‘Redlan’ and ‘Westland’ respectively showed 7%, 7% and 29% seed set in FC, while no seed set was

shown in the other experimental conditions. In A-line, ‘MS79’, ‘Reliance’, ‘Wheatland’ and ‘Tx624’ had no pollen fertility under all the four conditions. However, ‘CK60,’ ‘Martin,’ ‘Redbi-ne58’ and ‘Westland’ showed 5%, 7%, 16% and 61% pollen fertility in FC, respectively. ‘MS138’ showed 75% pollen fertility in MTC and 78% pol-len fertility in HTC, ‘Tx3048’ showed 36% in HTC and 67% in FC, and ‘Redlan’ showed 12% in MTC and 57% in FC despite 0% pollen fertil-ity in other experiments. In the experiments, a relatively high pollen fertility was observed either at a night temperature above 24℃ in MTC and HTC or at a day temperature above 29.3℃ in HTC and FC, however, their seed fertil-ity was considerably lower than the expected seed fertility from their pollen fertility in three conditions. Based on the results, A-lines can be classified into 4 groups, Group-1, Group-2, Group-3 and Group-4 (Table 4). ‘MS79,’ ‘CK60,’ ‘Martin,’ ‘Reliance,’ ‘Wheatland’ and ‘Tx624’ were classified into Group-1 by their stable male-ste-rility in various experimental conditions except for low pollen restoration of ‘CK60’ and ‘Martin’ in FC. ‘MS138’ was classified into Group-2 due to its moderate seed setting under minimum temperatures above 24℃, and ‘Tx3048’ was clas-sified into Group-3 due to its peculiar seed set-ting under maximum temperatures above 29.3℃ during a period from the 3rd to 10th leaf stages that corresponded to flower initiation of pollen development stages. ‘Redbine58,’ ‘Redlan’ and ‘Westland’ are classified into Group-4 by their seed setting with high pollen restoration that occurred only in FC.　Sixteen A-lines including 8 common lines in this study were evaluated for their seed fertility

－３－

Fluctuation of Male Sterility in Sorghum

Table 2. Average of maximum and minimum temperatures in a field at Sakai (Osaka Pref. Universi-

ty, Osaka, Japan) during the period between third and tenth leaf stages in 1999, and day

temperature (5:30 - 18:00) and night temperature (18:00 - 5:30) in three growth cabinets

in 1999.

Daily meanMinimum orMaximum orExperiment

temperaturenight temperatureday temperature

25.621.929.3Field condition (June 23 to July 28)

28.7 24.033.033℃/24℃ cabinet

24.024.024.024℃/24℃ cabinet

19.715.024.024℃/15℃ cabinet

in the field and greenhouse of Chugoku National Agricultural Experiment Station, Fukuyama, Japan (latitude 34°27’ N) . In the fields during the summer of 1965 (DMT of July 25.6℃), the percentage of seed fertility of ‘Reliance,’ ‘Redbi-ne58’ and ‘Westland’ was 13.9, 3.1 and 0.5%, respectively, although the other 13 A-lines were completely sterile (Tarumoto and Oizumi, 1967). In the greenhouse in summer and winter of 1965, all the eleven A-lines were completely sterile (Tarumoto, 1971). In FC of Fukuyama in 1965 and Sakai in 1999, ‘Redbine 58’ and ‘Westland’ usually showed variable male sterility, while ‘Reliance’ showed it only at Fukuyama in 1965. In the greenhouse at Fukuyama and in a growth cabinet at Sakai, ‘Redbine58’ and ‘Westland’ com-monly showed stable sterility, although the cause for the fluctuation of male sterility in ‘Red-bine58’ and ‘Westland’ was unknown. However, relatively high pollen fertility was observed in all A-lines belonging to Group-2 and Group-3 as well as in three A-lines of Group-4. Even though the influence of minimum and/or maximum tem-perature to male sterility is unknown in sorghum cultivars, the temperature stimulus is consid-ered to be related to phenotypic fluctuation of male sterility in Group-4, especially in ‘Redbi-ne58’ and ‘Westland.’ Clearly the fluctuation of male sterility was related to minimum tempera-tures above 24℃ in ‘MS138’(Group-2) and maximum temperatures higher than 29.3℃ in

‘Tx3048’(Group-3). Our results (Table 3 and Fig. 1) imply that relatively high pollen fertility is associated with the development up until the engorged pollen stage in high temperatures, and a small amount of pollen probably would rend and scatter. From the comparisons of gene expression in starch biosynthesis in developing pollen between fertile and male-sterile sorghum lines, Datta et al. (2001) indicated that tapetum and pollen developments proceed normally up to the starch filling stage in fertile and male ster-ile lines, and that pollen abortion occurs in the late stage of pollen development. In male-sterile corn, Lee et al. (1980) emphasized that since pollen development proceeds in a very late stage even in full sterile S lines, pollen abortion may be more easily averted through physiologi-cal or by environmental interactions. Thus, in certain male-sterile sorghum lines like ‘MS138’ and ‘Tx3048,’ the environmental condition dur-ing the early vacuolated pollen stage and the engorged pollen stage is considered to be criti-cal for conversion from sterile to fertile. How-ever, the observation of pollen is limited to revealing the above conversion, thus more ana-tomical studies are necessary for clarifying the relationship between environmental condition and pollen fertility.　Expression of male sterility can be satisfac-tory explained in Group-1 by the interaction between cytoplasm and nuclear-gene (Singh

－４－

Table 3. Percentage of seed fertility in self-pollination by paper bag and percentage of pollen fertil-

ity tested after stained with acetocarmine solution.

Pollen fertilitySeed fertilityA-lines

Field33℃/24℃24℃/24℃24℃/15℃Field33℃/24℃24℃/24℃24℃/15℃

00000000MS79

50000000CK60

70000000Martin

00000000Reliance

00000000Wheatland

00000000Tx624

3278750021190MS138

673600131500Tx3048

160007000Redbine58

5701207000Redlan

6100029000Westland

The samples resulted were collected fromplants grown in growth cabinets of 24℃/15℃, 24℃/24℃ and 33℃/24℃, and a field in 1999.

I. Tarumoto et al.:

and Hadley, 1961; Stephens and Holland, 1954), but this explanation is unsatisfactory in Group-2, Group-3 and Group-4 (Table 3). No differen-tiation of cytoplasmic type among eleven A-lines used in this study was confirmed by gene-specific PCR analysis on four mitochondrial m-RNA genes (COX2, COX3, COB and ORF25) (Adachi 2000 and Tarumoto et al., 2000) as was made known in Bailey-Serres et al. (1986). Since no structural modification exists among A1 cytoplasm in eleven A-lines, the presence of a modifier gene is assumable for the factor shap-ing the interaction between nuclear gene and A1 cytoplasm. Therefore, genes that fluctuate their relationship between sterile gene and A1 cytoplasm by high minimum and/or maximum temperature should be examined by further study.

Acknowledgement

　We are grateful to Dr T. Morikawa (Osaka Pref. University, Sakai, Japan) for his assistant and helpful discussion.

References

Adachi E. 2000. A survey of fluctuating expres-sion for sterility in cytoplasmic-nuclear male-sterile sorghum. (Master Thesis) Osaka Pre-fecture University, Sakai, Osaka (in Japanese with English summary)

Bailey-Serres J., Dixon L.K., Liddell A.D., and Leaver C.J. 1986. Nuclear-mitochondrial inter-actions in cytoplasmic male-sterile Sorghum. Theor. Appl. Genet. 73, 252-260.

Brooking I.R. 1979. Male sterility in Sorghum

bicolor (L.) Moench induced by low night temperature. II Genotype differences in sensitivity. Aust. J. Plant Physio. 6, 143-147.

Christensen J.E., Horner H.T., and Lersten N.R.

1972. Pollen wall and tapetal orbicular wall development in Sorghum bicolor

(Gramineae). Amer. J. Bot. 59, 43-58.Datta R., Chourey P.S., Pring D.R., and Tang H.V.

2001. Gene-expression analysis of sucrose-starch metabolism during pollen maturation in cytoplasmic male-sterile and fertile lines of sorghum. Sex Plant Report 14, 127-134.

Latha R., Senthilvel S., and Thiyagarajan K. 2004. Critical temperature and stages of fertil-ity alteration in thermo-sensitive genetic male

sterile lines in rice. Proc. 4th Intl. Crop Sci. Conf.(http://www.cropscience.org.au/icsc2004/3/

4/4/1089_latha.htm, Verified 11 August 2006)Lopez M.T., Toojinda T., Vanavichit A., and Tra-

goonrung S. 2003. Microsatellite markers flank-ing the tms2 gene facilitated tropical TGMS rice line development. Crop Sci. 43, 2267-2271.

Pring D.R., Conde M.F., and Schertz K.F. 1982. Organelle genome diversity in sorghum: Male-sterility cytoplasms. Crop Sci. 22, 414-421.

Pring D.R., Tang H.V., and Schertz K.F. 1995. Cytoplasmic male sterility and organelle DNAs of sorghum. In, The Molecular Biology of Plant Mitochondria (Levings C.S.Ⅲ and Vasil I.K., ed.) 461-495. Kluwer Acad. Pubs., Dordrecht, the Netherlands.

Scherts K.F. and Ritchey J.M. 1989. Cytoplasmic-genetic male-sterility system in Sorghum. Crop Sci. 18, 890-893.

Singh S.P. and Hadley H.H. 1961. Pollen abor-tion in cytoplasmic male-sterile sorghum. Crop Sci. 1, 430-432.

Stephens J.C. and Holland R.F. 1954. Cytoplas-mic male-sterility for hybrid sorghum seed production. Agron. J. 46, 20-23.

Tarumoto I. 1971. Studies on breeding forage sorghum by utilizing heterosis. Bull. Chugoku Nat. Agr. Exp. Sta. A19, 21-138. (in Japanese

－５－

Table 4. The relationship between level of male sterility and temperature during the period of

flower development in seed fertility, and the classification of A-lines according to the

relationship.

Temperature condition1)Level of male sterilityGroup (A-lines)

no concernstableGroup 1 (MS79, CK60, Martin, Reliance Wheatland, Tx624)

Temperatures > 24.0variableGroup 2 (MS138)

DMT>25.6 AMXT>29.3variableGroup 3 (Tx3048)

DMT>25.6 AMXT≦29.3variableGroup 4 (Redbine58, Redlan, Westland)

1) DMT: Daily mean temperature, AMXT: Average of maximum temperature

Fluctuation of Male Sterility in Sorghum

with English summary)Tarumoto I., Adachi E., Morikawa T., Yanase M.,

Fujimoto M., and Kasuga S. 2000. Differentia-tion of sterile expression and its factor in cyto-plasmic male-sterile lines in sorghum. Grassl. Sci. 46, (Suppl.) 118-119. (in Japanese)

Tarumoto I. and Oizumi H. 1967. Studies of for-age sorghum breeding. II The characteristics of male-sterile strains. Japan. J. Breed. 17,

276-282 (in Japanese with English summary)Xu G.W., Cui Y.X., Schertz K.F., and Hart

G.E. 1995. Isolation of mitochondorial DNA sequences that distinguish male-sterility-inducing cytoplasms in Sorghum bicolor (L.) Moench. Theor. Appl. Genet. 90, 1180-1187.

(Recieved May 16, 2007; Accepted March 15, 2008)

－６－

I. Tarumoto et al.: Fluctuation of Male Sterility in Sorghum

　著者らは，和歌山県紀伊半島南部，静岡県伊豆

半島，長崎県平戸・松浦地域の民家庭園における

維管束植物相を報告したが（道下ほか　２００４；道

下ほか　２００５；道下・山口２００６），一部について

その植物名と利用法を修正する。

　調査地で撮影した証拠写真に基づいて石井・井

上（１９６８－１９７１），佐竹ほか（１９８１－１９８２），佐竹

ほか（１９８９），塚本（１９８８－１９９０）を参照して記録

の重複と文献間の不一致を検証し，調査地での聞

き取り記録によって植物の利用法を精査した。

　その結果，和歌山県の民家庭園（道下ら　２００４）

では８種，静岡県の民家庭園（道下ら　２００５）で

は１４種，長崎県の庭園（道下・山口，２００６）では

１種を追加し（表１），関連の種を削除する。

　また種名と詳細情報を変更する必要のあるもの

は，和歌山県では１９種，静岡県では２３種，長崎県

では４種である（表２）。

　利用法については，和歌山県のセンダングサ，

トウバナ，カタバミ，ニワトコの４種を利用法無

し（雑草）に，静岡県のフユノハナワラビを観賞

に修正する。したがって，有用植物は，和歌山県

の民家庭園には５１８種，静岡県の民家庭園には６３６

種，長崎県の民家庭園には６４９種分布することに

なる。

引用文献

石井林寧・井上頼数　１９６８－１９７１．最新園芸大事

典（１巻－７巻）．誠文堂新光社．

道下雄大・梅本信也・山口裕文　２００４．紀伊半島

南部の民家庭園における維管束植物相　大阪府

大院農生学術報　５６，２９－４４．

道下雄大・梅本信也・山口裕文　２００５．伊豆半島

の民家庭園における維管束植物相．大阪府大院

農生学術報　５７，３３－５６

道下雄大・山口裕文　２００６．長崎県平戸・松浦地

域の民家庭園における維管束植物相．大阪府立

大学大学院生命環境科学研究科学術報告　５８，

　１３－３７

佐竹義輔・原寛・亘理俊次・冨成忠夫　１９８９．日

本の野生植物　木本，１巻－２巻．平凡社，東

京．

佐竹義輔・大井次三郎・北村四郎・亘理俊次・冨

成忠夫　１９８１－１９８２．日本の野生植物　草本，

１巻－３巻．平凡社，東京．

塚本洋太郎（監修）１９８８－１９９０．園芸植物大事典

（１巻－６巻）．小学館，東京．

－７－

大阪府大院生環学術報告59：７～９（２００８）．資料

要　旨

　西南日本の民家庭園における維管束植物で未同定であった種を追補し，一部の記録を修正す

る。庭園の有用植物は，和歌山県紀伊半島南部では５１４種，静岡県伊豆半島では６３６種，長崎県平

戸・松浦地域では６４９種となる。

Abstract

　There were 514 species in Kii-peninsula, 636 species in Izu-peninsula and 649 species in Hirado-Matsuura district as useful garden plants in the rural hamlets in Japan by means of con-firmation of picture records.

西南日本の民家庭園における維管束植物の追加・修正

Additional note and erratum of vascular plant flora in the home gardens in Southwestern Japan

道下雄大・梅本信也＊・山口裕文

（大阪府立大学大学院生命環境科学研究科資源植物多様性学研究室；

＊京都大学フィールド科学教育研究センター里域生態系部門）

－８－

道下・山口：

表１－１　新規に追加する種

文献３（長崎県）文献２（静岡県）文献１（和歌山県）

種名（和名/学名）利用鉢比率

集落利用鉢比率

集落集落

ＤＣＢＡＤＣＢＡＤＣＢＡ

観賞1000010Artemisia schmidtianaアサギリソウ

観賞00010Dahlia imperialisコダチダリア

観賞00100Euryops virgineusゴールデンクラッカー

0001Ixora chinensisサンタンカ

観賞00100Jasminum humile var. revolutumキソケイ

0010Coleus blumeiコレウス

0010Brunfelsia latifoliaニオイバンマツリ

1001Leptospermum scopariumギョリュウバイ

観賞00001観賞000021000Prunus glandulosaニワザクラ

観賞00010Aceriphyllum rosiiイワヤツデ

観賞1000100Crassula erosula cv. Campfireヒマツリ

観賞00001Lunaria annuaルナリア

観賞1000001Matthiola incanaストック

観賞10032101011Echinopsis eyriesiiタンゲマル

観賞1000001Gymnocalycium sp.ギムノカリキウム属の一種

観賞6743324053Mammillaria sp.マミラニア

0100Trochodendron aralioidesヤマグルマ

観賞501100Gasteria gracilisトラノマキ

観賞1000100Asparagus myriocladusタチボウキ

文献１：道下雄大・梅本信也・山口裕文　２００４．

文献２：道下雄大・梅本信也・山口裕文　２００５．

文献３：道下雄大・山口裕文　２００６

－９－

西南日本の民家庭園における維管束植物の追加・修正

表２　種名および詳細情報の修正

文献３文献２文献１修正前の種名種名（和名/学名）

○○リュウノウギクChrysanthemum morifoliumイエギク

○キク科の一種Chrysanthemum nipponicumハマギク

○キクイモHelianthus tuberosusヒメヒマワリ

○カイザイクHelichrysum bracteatumムギワラギク

○○アキタブキ，マルバダケブキPetasites japonicusフキ（3倍体）

○キキョウ科の一種Campanula poscharskyanaアルペンブルー

○モクセイ科の一種Syringa vulgarisライラック

○ヒメジソIsodon inflexusヤマハッカ

○カリガネソウClerodendrum ugandenseブルーエルフィン

○○ローズゼラニウムPelargonium × asperumニオイゼラニウム

○○ヒメツゲBuxus microphyllaツゲ

○ユスラウメIlex serrataウメモドキ

○ザクロPunica granatum var. nanaヒメザクロ

○ビジョザクラCuphea hyssopifoliaメキシコハナヤナギ

○カイドウMalus hallianaハナカイドウ

○ピラカンサPyracantha crenulataヒマラヤトキワサンザシ

○○エケベリア，コモチレンゲGraptopetalum paraguayenseオボロヅキ

○イワレンゲOrostachys japonicusツメレンゲ

○ケラマツツジRhododendron obtusum var. kaempferiヤマツツジ

○ハナダイコンOrychophragmus violaceusオオアラセイトウ

○○ベゴニア，レックスベゴニアBegonia semperflorensシキザキベゴニア

○マンテマ属の一種Silene dioicaレッドキャンピオン

○柱サボテン類Marginatocereus marginatusハクウンカク

○サボテン科の一種１Opuntia vulgarisタンシウチワ

○○キリシマミズキ，コウヤミズキCorylopsis spicataトサミズキ

○コバンモチTrochodendron aralioidesヤマグルマ

○トリカブトAconitum japonicumヤマトリカブト

○カザグルマClematis sp.センニンソウ属の一種

○ラン科の一種Cattleya sp.カトレア属の一種

○イキシアBabiana strictaホザキアヤメ

○○カキツバタ，ハナショウブIris cv.ダッチアイリス

○スプランテラSparaxis tricolorスイセンアヤメ

○キジカクシAsparagus densiflorusスギノハカズラ

○○アスパラガス，アスパラガス類Asparagus plumosusシノブボウキ

○スズランConvallaria majalisドイツスズラン

○ノカンゾウHemerocallis fulva var. kwansoヤブカンゾウ

○ミヤマナルコユリPolygonatum falcatumナルコユリ

文献１：道下雄大・梅本信也・山口裕文　２００４．

文献２：道下雄大・梅本信也・山口裕文　２００５．

文献３：道下雄大・山口裕文　２００６．

○：関連する文献

（２００８年５月１０日受領；２００８年６月１０日受理）

Ⅰ．欧文原稿の作成方法

　　（原著論文original paper 及びその他の記事）

　

１．Ａ４版（210mm×297mm）またはこれに近い大きさのタイプ用紙に，ダブルスペースで印字する。

用紙の周囲に約３㎝の余白を残す。

２．原稿の第１ページには，論文題名，著者名，所属

を記載し，下端にRunning Title（奇数ページの上

に見出しとして印刷される）を以下のように記載

する。

Ａ）論文題名：冠詞，前置詞，接続詞を除く語の頭

文字を大文字とする（学名など規定のある場合

を除く）。第○報の場合には，１，２，３のように

する。

記載例：

The Waltzing Mutant in the Shrew (Suncus

murinus, Insectivora) Characterized by the

Circling and Head-Shaking Behaviors 1.

Inheritance and Breeding.

Ｂ）著者名：名，姓の順（外国人が著者の場合はそ

の国の習慣に従う）にローマ字で表記する。著

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名の場合）

　Ichiro KAWASAKI, Jiro YAMADA and Saburo

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Kawasaki et al.：Waltzing Behavioral Mutant of

Suncus murinus-1

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４．本文は第３ページからタイプする。本文中の引用

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et al., 1995a; Yamaguchi and Mochioka, 1996)の

ように記入する。

５．図・表の表題はFig 1. Location of …およびTable

2. Various cultivars of…のように記入し，図の表

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よい。図の縮小率（長さ）を特に希望する場合は

欄外に赤字で記す。

図表の挿入場所は本文の該当場所の右欄外に赤字

で明記する。

６．引用文献（References）は著者名をアルファベッ

ト順に配列し，次の例にならって記載する。著者

名，表題，誌名（書名）とも省略しない。

記載例：

　Oda M., Dosai M., Ikeda H., and Furukawa

H. 2002. Causes of low survival in cucumber

(Cucumis sativus L.) plants grafted onto

pumpkin (Cucurbita moschata Duch.) root-

stocks by horizontal-cut grafting at the center of

the hypocotyl. Sci. Rep. Grad. Agric. Sch. Biol.

Sci., Osaka Pref. Univ., 53, 1-6. (in Japanese)（一

般論文の引用例）

　Snedecor G. W. 1961. Statistical Methods.

Iowa State Univ. Press, Iowa, p.13.（単行本の１

ページのみを引用した例）

　Tanaka T. 1977. Nogyo Mondai. Fudaisha

Sakai, 215pp. (in Japanese)（単行本全体を参考に

した例）

　Hendricks S. B. and Bortwhick H. A. 1963.

Control of plant growth by light. In “Environ-

mental Control of Plant Growth” (ed. Evans

L.T.). Academic Press, New York, 233-264. （編集

者の有る本の１章を引用した例）

７．その他，原稿のスタイルとレイアウトについて

は，最新の号を参照すること。

Ⅱ．和文原稿の作成方法

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２．記述は新かなづかいに従い，学術用語以外は原則

として当用漢字を用いる。句読点は「， 。」を用

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白河紀男　１９７０．除草剤CMMPに関する研究（第

４報）．特に作用機作について．園学雑，３８，１９３

－２０１．

平井篤造・鈴木直治　１９６３．植物病理の生化学（後

編）．農業技術協会，１５５－１８０．

Instructions for Authors　執筆要領

－　i　－

大阪府立大学大学院農学生命科学研究科

学術報告第５９巻

平成２１年３月３１日

発行者　〒５９９-８５３１　堺市中区学園町１－１

大阪府立大学大学院生命環境科学研究科

印刷所　オフィスランド・ハツブン㈱

Contents

Original Paper

Tarumoto, I., E. Ishii, M. Yanase and M. Fujimori　The Phenotypic Fluctuation Factor for Male Sterility in　A1 Male Sterile Lines of Sorghum (Sorghum bicolor Moench) …………………………………　1

Notes

Michishita, Y., S. Umemoto and H. Yamaguchi　Additional Note and Erratum of Vascular Plant Flora in the Home Gardens in　Southwestern Japan …………………………………………………………………………………………　7

Instructions for Authors ……………………………………………………………………………………　i　

Scientific Report

of

the Graduate School of Life and Environmental Sciences,

Osaka Prefecture University

Vol. 59 2008

Published by

the Graduate School of Life and Environmental Sciences,

Osaka Prefecture University

Sakai, Osaka, Japan