1

F H15-004

The metal nano-pattern formation

Hitoshi Tabata

ISIR-Sanken, Osaka University

Si

DNAAuDNADNApoly[d(G-C)]poly[d(G-C)] DNA BAVD

IS-FET DNA Self-assemble properties of DNA and bio based molecules were studied by use of SPM with Si as a substrate. By use of an ethanol, DNA network was successfully stabilized on a mica substrate. Formation of DNA networks are confirmed also on various substrate other than mica, i.e. silicon wafer, pyrolytic carbon etc. Conditions to control size of meshes were also found. This scaffold is a candidate for bio-nano electronics and bio-chips. New type non-fluorescent molecules labelled DNA-chips have been constructed by using IS-FET devices. For creating nano-patterns, we have developed bottom-up nano-fabricating apparatus such as nano-imprinting and nano-inkjet.

DNAIS-FET DNA DNA DNA

DNA DNA DNA IS-FET DNA DNA DNA

DNA IS-FET DNA Fig.1 IS-FETprobeDNA targetDNA DNA DNA gate

2

DNA

100 nm

Fig.2 DNA DNA DNA DNA

Fig.3 SEM 100nm

Fig.3 SEM images of metal nano-electrodes IS-FET DNA JST-CREST [1] DNA 52 [3] (2003) 126-129. [2]DNA M&E12 (2003) 168-175. [3]DNA

Fig.1 The non- labeling type DNA sensor using IS-FET.

Fig.2 The fluorescence microscope photograph of DNA with the patterning.

p pn n

10m

SiO2(1m)

AlSi3 N4(1000)SiO2(1000)

p

p pn n

10m

SiO2(1m)SiO2(1m)

AlSi3 N4(1000)SiO2(1000)

p

DNA

3

6 (2003) 78-79. [4]DNA Nano- electronics: Physical and Electrical

Properties of DNAHitoshi Tabata, Tomoji Kawai, The 7th World Multi-Conference on Systemics, Cybernetics and Informatics (SCI2003) 2003.7.27-30 [5] Interdisciplinary Nano-Technology of Bottom-Up and Top-Down for DNA and Bio-Electronics H.Tabata, T.Ohtake, T.Uno, Y.Otsuka and T.Kawai, International Workshop on Plasma Nano-Technology and Its Future Vision, 2004.2.5-6 [6]DNA Nano-patterning with Self-organization by using Nanoimprint T.Ohtake, K.Nakamatsu, S.Matsui, H.Tabata and T.Kawai, 7 2 21 COE 2004.1.13-14 [7]Direct Detection of DNA using ISFET based on PNAT.Uno, T.Ohtake, H.Tabata and T.Kawai 7 2 21 COE 2004.1.13-14 [8]DNADNA , 2003.4.24 [9] 2003.5.27 [10] , AMPI 2003.11.13 [11] , 387 2003.12.12 [12]PNA-ISFETDNA , 2004 16 51 2004.3.28-31 [13] DNA , 2004 16 51 2004.3.28-31