RNA From RNA-Bee MasterPure Complete DNA and RNA Purification Kit NucleoSpin RNA II NucleoSpin RNA L
From LCM sample NucleoSpin RNA XS ArrayPure Nano-scale RNA Purification Kit
From MasterPure Yeast RNA Purification Kit
Small RNA Application NucleoSpin miRNA
From NucleoSpinRNA Plant MasterPure Plant RNA Purification Kit
From FFPE sample WaxFree RNA NucleoSpin FFPE RNA QuickExtract FFPE RNA Extraction Kit
QuickExtract FFPE RNA Extraction Kit QuickExtract RNA Extraction Kit
mRNA Isolation NucleoSpin mRNA MiniMidi mRNA-ONLY Prokaryotic/Eukaryotic mRNA Isolation Kit
RNA Clean-Up NucleoSpin RNA Clean-UpRNA Clean-UP XS
NucleoSpin Triprep( RNA/DNA/Protein) NucleoSpin RNA/Protein
RNARNA
BioBio--Genesis Technology Genesis Technology
P.1 - P.3
P.4 P.5 - P.6
P.7 - P.8 P.9
P.10 P.11
P.12
P.13 - P.14
P.15 - P.16 P.17
P.18 P.19 - P.20
P.21
P.21
P.22
P.23 P.24
P.25 - P.26
P.27
P.28 P.29
RNA
RNA RNA
-80oC RNA RNase
RNA RNA
RNase RNA RNA (RNA
extraction kit)
RNA Guanidinium thiocyanate RNase
phenol-chloroform (pH=4.5) RNA single reagent (ex: RNAzol Bee)
Single reagent RNA
single reagent -80oC< 1 month RNA RNA
RNA single reagent RNA
RNA genomic DNA phenolchloroform
RNA
RNA
RNA silica column RNA
RNA
Silica column silica
silica column RNA DNase I genomic DNA
wash buffer
RNASilica column RNA column silica
column RNA column nucleotide fragment size binding capacity
Epicentre MasterPure RNA extraction kit silica column
MasterPure lysis buffer proteinase K
isopropanol nucleotide RNase-Free DNase I
DNA total RNA silica column MasterPure
RNA
RNA RNA TE buffer TE buffer EDTA RNA
RNA DEPC-treated water nuclease-free water-
80oC RNA RNase
RNA RNA RNase inhibitor (ex: RiboGuard RNase in-
hibitor) RNA
RNA RNA RNA RNA
RNA RNA
RNA
1.
RNA 230nm260nm 280nm 260nm
DNA RNA280nm 230nm A260
RNA (RNA concentration = A260 x dilution fold x 40 ng/ul) RNA
A260/A280 ratio 1.9~2.1 A260/A230 ratio 2~2.4 A260/A280 1.9
sample
RNA RNA
A260 RNA
2.
RNA sample
genomic DNA rRNA RNA
RNA
RNA
RNase-Free RNase RNA
RNA single-strand nucleotide
RNA rRNA 0.5x TBE
agarose gel RNA size
formaldehyde denature agarose gel
RNA P.2P.2
3. RNA Integrity Number (RIN)
RNA Integrity Number RNA RNA
RNA RINRIN
1~10 RNA
RNA A. silica column solution method
RNA silica column
RNA solution method
B.
RNA BeeNucleoSpin RNA II or MasterPure RNA purification kit
NucleoSpin RNA XS or ArrayPure Nano-Scale RNA purification
kit
NucleoSpin Plant or MasterPure Plant purification kit
FFPE sample NucleoSpin FFPE or QuickExtract FFPE RNA extraction kit
C.
miRNA assay NucleoSpin miRNA
QuickExtract RNA extraction kit
RNA Clean-Up NucleoSpin RNA Clean-Up or RNA Clean-Up XS
mRNA isolation NucleoSpin mRNA Mini or mRNA Midi
Fig.1 RNA Integrity Number (RIN)
Total RNA RIN 10 RIN 2 RIN
10 sample 18S 28S rRNA
RIN rRNA RNA RIN
2 rRNA RNA
RNA
RIN Value Most Suitable Application
1 ~ 4 PCR assays with short region of amplification
4.1 ~ 6.9 qRT-PCR applications
7~10 Highly demanding gene array assays
RNA RIN
RNA P.3P.3
Traditional RNA Isolation
RNA-Bee single reagent RNA 1
/
1ml RNA-Bee 50 mg 5 x 106 100 l whole blood 109
MN NucleoSpin RNA Clean-up or NucleoTrap mRNA
Ordering Information
Tel-Test RNA-Bee 200ml LB-CS-105B
RNA-Bee easy protocol
RNA-Bee phenol quanidine thiocyanate
single reagent RNA-Bee
chloroform //
DNA
RNA isopropanol RNA
RNA pellet RNase-Free water
-70oC RNA reverse transcrip-
tionRT-PCRNorthern Blot
From P.4P.4
MasterPure Complete DNA and RNA Purification Kit
MasterPureTM Complete DNA and RNA purification kit total nuclei acid
(TNA)DNA RNAMasterPure
TNA DNase I total
RNA RNase A genomic DNA RNA gDNA kit
30min TNA60min total RNA column
phenolchloroform
(include
Fig.3 MasterPure sample(M= 1kb ladder)
Ordering Information
MasterPure Complete DNA and RNA
Purification Kit
For 10 gDNA or 10 TNA or 5 RNA purifications MC89010
For 200 gDNA or 200 TNA or 100 RNA purifications MC85200
MasterPure RNA Purification Kit 100 RNA purifications MCR85102
MasterPure DNA Purification Kit
(for isolating TNA or DNA) 200 gDNA or 200 TNA purifications MCD85201
Sample Sample Size TNA (g) DNA (g)
RNA (g)
HeLa/HL60 cells 1 X 106 cells 10-30 3-12 7-15
Tissues
Liver 5 mg 33-42 5-10 13-25
Brain 5 mg 9-13 6-9 4-11
Heart 5 mg 6-10 4-7 4-5
Kidney 5 mg 10-17 3-8 14-17
Thymus 5 mg 15-30 6-12 9-18
Blood 200 l 3-10 3-9
Buffy coat 300 l 40-55 40-55 3-6
Mouse tail 0.5 cm 25-30 9-11
E. coli 3.5 x 106 cells 2.5-2.8 1.3-1.6 1.6-1.8
S. mutans 1.5 ml 0.9
Yeast (S. cerevisiae)
2.2 x 106 cells 11-18
1.1 x 107 cells 70-78
Table.2 MasterPureTM Complete DNA and
RNA purification kit
Targets Samples Extracted
Human & mammalian RNA & genomic DNA HIV HCV RSV HPV Yeast Enterovirus B. pertussis E. coli M. tuberculosis Streptococcus mutans Soy (tofu) Maize Insect tissues
Plasma Whole blood Guthrie cards Buccal cells Liver Mouse tail Kidney Saliva Urine Sputum Tissue-culture cell lines Cervical cells Paraffin-embedded tis-sues
Table.1 MasterPureTM Complete DNA and RNA purifica-
tion kit
From P.6P.6
NucleoSpin RNA II NucleoSpin Filter RNase-Free DNase column genomic DNA
DNA
(G+/G bacteria) RNA
phenol/chloroform
30 total RNA
Fig.2 MN NucleoSpin RNA II Q
Total RNA 106 HeLa cells MN NucleoSpin RNA II (Lane 1-3) Q RNA kit
(Lane 4-6) elute 50ul 10ul 1.2% Formadelhyde gel
Fig.1 Total RNA
1.2ml LB cultured Pseudomonas putida (OD600=0.9)
0.05mg/ml (1&4), 0.2mg/ml (2&5) and 0.8mg/ml
(3&6) lysozyme 37oC 10min NucleoSpin RNA II
total RNA 6ul total RNA 1.2% formaldehyde gel
A260/280 2.1
From P.7P.7
Fig.3 ()MN NucleoSpin RNA II 10 cells total RNA RT-PCR
MN NucleoSpin RNA II 105, 104, 103, 330, 100, 33, 10, 3 0 HeLa cell
total RNA (Lane 1~9) 3ul total RNA b-actin primer RT-PCR 15ul 1.2%
agarose gel 10 HeLa cellsNucleoSpin RNA II RT-PCR
total RNA
Ordering Information
NucleoSpin RNA II 10 reactions 740955.10
50 reactions 740955.50
250 reactions 740955.250
Fig.4 Genomic DNA Q
Total RNA 106 HeLa cells MN NucleoSpin
RNA II (Lane 1-3) Q RNA kit (Lane 4-6) 10ul
total RNA RNAse A 1% TAE agarose gel
genomic DNA Q Dnase I total RNA
samplegenomic DNA MN NucleoSpin
RNA II
Technology Silica membrane technology
Format Mini spin columns
Sample material < 5 x 106 cultured cells, < 109 bacterial cells
< 108 yeast cells, < 30 mg tissue
Fragment size > 200 b
Typical yield 14 g from 106 HeLa cells
70 g from 109 bacterial cells
A260/280 1.9-2.1
Typical RIN (RNA integrity number) > 9
Elution volume 40-100 l
Preparation time 30 min/6 preps
Binding capacity 200 g
Product Information
Fig.5 rDNase MDB (membrane desalting buffer) genomic
DNA
NucleoSpin RNA II total RNA MDB (+MDB)
MDB (-MDB) rDNase DNA contaminant assay
qPCR PCR product agarose gel sample
DNA PCR rDNase MDB
gDNA contamination
From P.8P.8
NucleoSpin RNA L 200mg total RNA
600ug RNA
phenol/chloroform
(G+/G bacteria)
Technology Silica membrane technology
Format Midi spin columns
Sample material < 5 x 107 cultured cells, < 200 mg tissue
< 1010 bacterial cells, up to 3 x 108 yeast cells
Fragment size 200b ~ 20Kb
Typical yield 180 g from 107 HeLa cells
620 g from 4 x 107 HeLa cells
A260/280 1.9-2.1
Typical RIN (RNA integrity number) > 9
Elution volume 500 l
Preparation time 80 min/4 preps
Binding capacity 700 g
Product Information
NucleoSpin RNA L 20 reactions 740962.20
Fig.1 NucleoSpin RNA L total RNA
Pig liver & kidney NucleoSpin RNA L Q
total RNA
A: RNA isolated from 100mg (1~4) and 200mg (5~8) pig
kidney.
Lane 1,2,5,6: NucleoSpin RNA L
Lane 3,4,7,8: Competitor Q
B: RNA isolated from 200mg pig liver sample.
Lane 1,2,3: NucleoSpin RNA L
Lane 4: Blank
Lane 5,6,7: Competitor Q
From P.9P.9
Ordering Information
NucleoSpin RNA XS
0.1mg total
RNA
Technology Silica membrane technology
Format XS spin columns
Sample material Tissue 200b
Typical yield 102 HeLa cells: 0.1~1.5ng ; 103 HeLa cells: 10~15ng
104 HeLa cells: 100~150ng ; 105 HeLa cells: 1000~1500ng
A260/280 1.9-2.1
Typical RIN
(RNA integrity number) > 9 (Depending on sample quality)
Elution volume 5-20 l
Preparation time 40 min/12 preps
Binding capacity 90 g
Product Information
Total RNA Extraction
NucleoSpin RNA XS 10 reactions 740902.10
50 reactions 740902.50
250 reactions 740902.250
Ordering Information
Tissue or Cell
Source
Amountof Start-
ing Material
NucleoSpin RNA XS
SampleNo. 1 (ng/ l)
NucleoSpin RNA XS
SampleNo. 2 (ng/ l)
Competitor Q Sam-
pleNo. 1 (ng/ l)
Competitor Q Sam-
pleNo. 2 (ng/ l)
Mouse Kidney 0.5 mg 157 108 96 63
Mouse Kidney 0.1 mg 27 25 17 18
HeLa Cells 1,000 cells 1.3 1.7 0.08 0.06
Table.1 sample NucleoSpin RNA XS Q total RNA
ArrayPure Nano-scale RNA Purification Kit
ArrayPureTM Nano-scale RNA purification kit (1-104 cells) RNA pu-
rification kit RNA column
LCM sample total RNA real-time RT-PCR 2-round aRNA synthesis
for microarray analysis
Fig.1 ArrayPureTM Nano-scale RNA purification kit 102101 HeLa
cells total RNA(3)RNA MMLVcDNAreal-time
PCR
Fig.3 Fig.1 data standard curveSlope=-3.14R2=0.992
Fig.2 ArrayPureTM Nano-scale RNA purification kit
flask HeLa cell RNA label
Cy3Cy5 microarray analysis
0.946
Ordering Information
10 RNA purifications MPR09010
100 RNA purifications MPR09100
: Nano-scale Lysis Solution, 2X Nano-scale Lysis Solution, MPC Protein Precipitation Reagent, Proteinase
K, RNase-Free DNase I, RiboGuard RNase Inhibitor, 1X DNase Buffer, TE Buffer.
ArrayPure Nano-scale RNA Purification Kit
From LCM Sample P.11P.11
MasterPure Yeast RNA Purification Kit
MasterPureTM Yeast RNA purification kit
RNA
phenol
RNA
1ml S.cerevisiae 25~50ug RNA
cDNA synthresismicroarray gene expression assay
Quality of obtained using the MasterPure Yeast RNA Purifica-
tion Kit.
MasterPure Yeast RNA Purification Kit S.cerevisiae
-20oC 2 Agilent Technologies 2100 Bioanalyer
MasterPure Yeast RNA Kit
RNA RNA
Microarray analysis of yeast RNA purified with the MasterPure Yeast
RNA Purification Kit.
S.cerevisiae MasterPure Yeast RNA Purification Kit RNA
reverse transcriptionlabeling hybridized to S. cerevisiae DNA mi-
croarray
Ordering Information
10 RNA purifications MPY83010
100 RNA purifications MPY83100
: Extraction Reagent for RNA, MPC Protein Precipitation Reagent, TE Buffer (in 100-reaction kit only), Pro-
teinase K, RNase-Free DNase I, RiboGuard RNase Inhibitor, 10X DNase Buffer, 2X T & C Lysis Solution.
MasterPure Yeast RNA Purification Kit
From P.12P.12
NucleoSpin miRNA Small RNALarge RNA
RNA
Small RNA (18~200 bases)
Small RNA (18~200 bases) Large RNA(>200bases)
total RNA ( Small RNA Large RNA)
SDS-PAGE Western Blot
Phenol/Chloroform RNA
chaotropic salt
spin-column
NucleoSpin Filter:
NucleoSpin Protein Removal Columns: RNA
rDNase: column genomic DNA
Fig.2 Agilent Bioanalyzer MN NucleoSpin miRNA total RNASmall RNA Large RNA
107 HeLa cell NucleoSpin miRNA RNA total RNA (A)small RNA (B) arge RNA (C)NucleoSpin miRNA
total RNA small RNA large RNA
Fig.3
NucleoSpin
miRNA 104
105106107
HeLa cell total
RNA MiR-
16 qPCR
total RNA
qPCR
Ct RNA
Fig.4
NucleoSpin miRNA
Laemmli-like PSB
40ug 12% SDS-PAGE
1. mouse liver
2. mouse kidney
3. mouse spleen
4. mouse lung
5. mouse heart
6. porcine liver
7. human brain
8. HeLa cell
Fig.1 2.5ug, 5ug, 10ug, 15ug RNA DICER enzyme small RNA
NucleoSpin miRNA large dsRNA small siRNA 1%
TAE agarose gel
1,12:100bp DNA marker 2,11:unpurified DICER reaction mix
3-6: 25ul purified large dsRNA 7-10: 25ul purified small RNA
Small RNA Application P.13P.13
Small RNA only Small RNA and Large RNA Total RNA
miRNA
Technology Silica membrane technology
Format Mini spin columns
Sample material < 107 culture cells, < 30mg human/animal tissue
< 50mg plant tissue, < 150ul reaction mixture
Fragment size for Small RNA 18 - 200 bases
Fragment size for Large RNA >200 bases
Typical yield 10ug small RNA, 95ug large RNA from 107 HeLa cells
Binding capacity 200g
Elution volume 30 - 100 l
Preparation time < 45 min (6 preps human/animal tissue, small and large RNA)
< 35 min (6 preps human/animal tissue, small RNA)
Product Information
Ordering Information
NucleoSpin miRNA 10 reactions 740971.10
50 reactions 740971.50
250 reactions 740971.250
Fig.5 NucleoSpin miRNA gDNA Q A
107 HeLa cells NucleoSpin miRNA
Q A miRNA extraction
qPCR 200bp ATPase 6 gene
NucleoSpin miRNA gDNA Ct A (
Ct=3.5) Q ( Ct=4.3) A Q gDNA
NucleoSpin miRNA 10
MN Q A
Small RNA ApplicationP.14P.14
miRNA Extraction
NucleoSpin RNA Plant
Plant RNA Extraction
RNA
Total RNA
2 lysis buffer (RA1RAP)
70ug RNA
Real-Time RT-PCRprimer extensionarray technologyRNase protection as-
say
Lanes: Average Yield
1-3: poppy seed lysed with buffer RA1 0.84ug
4-6: poppy seed lysed with buffer RAP 7ug
Fig.4 NucleoSpin RNA Plant 2 lysis buffer
lysis buffer RNAMN NucleoSpin RNA Plant RA1
RAP lysis buffer
MucleoSpin RNA Plant 50mg RA1 (Lane 1~3) RAP (Lane 4~6) lysis
total RNA 20ul total RNA 1.2% Formaldehyde agarose gel RAP
lysis RA1 total RNA
Fig.1 NucleoSpin RNA Plant RT-PCR inhibitor
ex: RT-PCR
16 RT-PCR 8 50mg NucleoSpin
RNA Plant RNA 60ul elute 3ul total RNA RT
-PCR 15ul PCR product 1.2% agarose PCR product
200bp.
M: marker 1,2: Tobacco 3,4: Tomato 5,6: Red pepper 7,8: Zucchini
9,10: eggplant 11,12: Broccoli
13,14: Radish 15,16: Maize
Fig.2 NucleoSpin RNA Plant sample total RNA
50mg NucleoSpin RNA Plant total RNA
1% Formaldehyde gel
Lane 1: maize,
Lane 2: thyme,
Lane 3: Palma christi
Lane 4: tobacco,
Lane 5: rye,
M: Marker.
Fig.3 NucleoSpin RNA Plant Q
100mg NucleoSpin RNA Plant Q
total RNA 100ul 10ul 1.2% formaldehyde
agarose gel A260/280 1.8~2.1
NucleoSpin RNA Plant RNA Q
From P.15P.15
Technology Silica membrane technology
Format Mini spin columns
Sample material 1-100mg tissue
Fragment size 200 b - 20kb
Typical yield 3-70ug from 100mg plant material
A260/280 1.9-2.1
Elution volume 60 l
Preparation time 30 min/6 preps
Binding capacity 200 g
Product Information
Ordering Information
NucleoSpin RNA Plant 10 reactions 740949.10
50 reactions 740949.50
250 reactions 740949.250
NucleoSpin RNA Plant
Table.1 50mg total RNA
From P.16P.16
MasterPure Plant RNA Purification Kit
MasterPureTM Plant RNA purification kit Plant Tissue and Cell Lysis Solution
WaxFreeTM RNA xylene
column resin RNA RNA
2hr
FFPE samplefresh or frozen tissuecultured cells
RT-PCRReal-Time RT-PCR
WaxFreeTM RNA FFPE sample RNA extraction kit silica column
RNA RNA binding elute RNA
WaxFree RNA Q-solution paraffin enzyme mix R-resin
DNA RT-PCR total RNA
WaxFree RNA
WaxFree RNA extraction from lung tissue FFPE section
WaxFree RNA FFPE sample (size: 1x1 cm5um thickness)
total RNA 20ul 50ul RT RT 15ul 50ul PCR
primer EGFR primer setPCR 195bp
Lane 1:100bp ladder;
Lane 2: NTC
Lane 3: gDNA control; .
Lane 4: WaxFree RNA extract
Lane 5: WaxFree RNA extract without tissue
Lane 6: Commercial total RNA (positive control)
Ordering Information
WaxFreeTM RNA 50 reactions WR-50
From FFPE Sample P.18P.18
-RNA Extraction from paraffin sample
1ul RT product 30ul qPCR reaction
Paraffin Dissolver paraffin xylene 3
paraffin
Decrosslinking bufferrDNase DNA
XS mini column 5ul RNA RNA
70
RT-PCRReal-Time RT-PCR
FFPE RNA DNA NucleoSpin FFPE RNA/DNA
NucleoSpin FFPE RNA
Table.1 Q R NucleoSpin FFPE RNA
RNA quality
From FFPE Sample P.19P.19
NucleoSpin FFPE RNA 10 reactions 740969.10
50 reactions 740969.50
250 reactions 740969.250
50 reactions 740978.50
NucleoSpin FFPE RNA/DNA 10 reactions 740978.10
250 reactions 740978.250
Paraffin Disslover 25ml 740968.25
Ordering Information
Technology Silica membrane technology
Format Mini spin columns - XS design
Sample material Up to 7 sections (10um)of 250mm2 or 15mg paraffin
Typical yield Depend on amount and quality of sample
A260/280 1.9 - 2.1
Typical RIN 2 - 6 (Strongly depend on sample quality)
Elution volume 5 - 30 l
Preparation time ~70 min/6 preps
Binding capacity 60 g
Product Information
From FFPE Sample P.20P.20
FFPE RNA FFPE RNA Extraction
7.5mm FFPE
RNA
NucleoSpin FFPE RNAQ
R kit MN
NucleoSpin FFPE RNA
RNA
RNA RNA Q
R
QuickExtract FFPE RNA Extraction Kit
32 FFPE sample RNA
xylenephenol
DNase I
RT-PCR
Fig.1 QuickExtractTM FFPE RNA
Extraction kit FFPE sample from
skeletal muscle RNA MMLV
cDNA PCR
primer set PCR
Lane M, 100-bp DNA ladder;
Lane 1, a 116-bp region of RYR1;
Lane 2, a 162-bp region of ACTA;
Lane 3, a 166-bp region of RSP18;
Lane 4, a 166-bp region of ENO3;
Lane 5, a 226-bp region of GAPDH;
Lane 6, a 232-bp region of OAZ1;
Lane 7, a 307-bp region of ACTB;
Lane 8, a 308-bp region of TNF.
Ordering Information
5ml (50 reactions) QFR82805
50ml (500 reactions) QFR82050
QuickExtract FFPE RNA Extraction Solution, DNase buffer 1, RNase-Free DNase I, Stop Solution.
QuickExtractTM FFPE RNA Extraction kit
QuickExtractTM FFPE RNA Extraction kit
Tabel.1 QuickExtract FFPE RNA
QuickExtractTM FFPE RNA Extraction kit RNA extraction kit from FFPE sample
FFPE sample xylene paraffin RNA degradation
QuickExtractTM FFPE RNA Extraction kit paraffin cross
-linking 35
From FFPE Sample/ P.21P.21
QuickExtract RNA Extraction Kit
1 total RNA
phenolchloroform
DNase I
Fig.1 105 HeLa cell 100ul Quickextract RNA solution vortex 1 RNA 1ul RNA
lysate MMLV cDNA 2ul cDNA template p532 6 primer sets
PCR
Lanes M: 100-bp ladder
Lane 1: 12,984-13,892
Lane 2: 9,406-10,202
Lane 3: 5,194-5,802
Lane 4: 4,191-4,690
Lane 5: 2,280-2,676
Lane 6: 1,029-1,329
Fig.2 RNA extraction kit
RNA MMLV cDNA ALDOA primer set PCR
Lane M, 100 bp ladder ;
Lanes 1 and 2, QuickExtract RNA Extraction Kit;
Lanes 3 and 4, kit from Competitor 1;
Lanes 5 and 6, kit from Competitor 2.
Ordering Information
5ml (50 reactions) QER09015
50ml (500 reactions) QER090150
QuickExtract RNA Extraction Solution, DNase buffer 1, RNase-Free DNase I, Stop Solution, RiboGuardTM
RNase Inhibitor.
QuickExtractTM RNA Extraction kit
QuickExtractTM RNA Extraction kit RNA 103~105 cells 100ul Quickextract
RNA solution vortex 1 RT-PCR
P.22P.22
NucleoTrap mRNA Mini/Midi
NucleoSpin mRNA Mini NucleoSpin mRNA Midi
Technology Affinity Chromatography Affinity Chromatography
Format Oligo (dT) latex-beads suspension Oligo (dT) latex-beads suspension
Sample material 250 g Total RNA 1000 g Total RNA
Fragment Size 50b - 20Kb 50b - 20Kb
Typical yeild 10 g mRNA 40 g mRNA
A260/280 1.9 - 2.1 1.9 - 2.1
Elution Volume 10 - 20 l 10 - 20l
Preparation Time 30min / 6 preps 30min / 6 preps
Binding Capacity 5 g poly(A) mRNA/mg beads 5 g poly(A) mRNA/mg beads
Product Information
Ordering Information
NucleoSpin mRNA Mini 12 reactions 740655
NucleoSpin mRNA Mini 12 reactions 740656
oligo (dT) latex-beads mRNA
cultured cell mRNA RNA
30 gDNA mRNA
mRNA isolationRT-PCRin vitro transcription
mRNA isolation
MN NucleoTrap mRNA Kit Q S
mRNA
500ug total RNA from mouse kidney, Lane 1 & 2
Q mRNA kitLane 3 & 4NucleoTrap mRNA Kit
Lane 5 & 6 S mRNA KitLane 7 & 8
mRNA 2.5ug RNA 1.5% Formaldehyde agarose
gel 4.5-kb parkin gene transcript
(Parkinsons Disease) 32P-Labeled DNA
probe Northern Blot NucleoTrap
mRNA kit mRNA Q S
mRNA Isolation P.23P.23
mRNA-ONLY Prokaryotic/Eukaryotic mRNA Isolation Kit mRNA-ONLY Prokaryotic mRNA Isolation Kit with poly(A)-Tailing
mRNA rRNA RNA amplification
poly(A)-Tailing kit mRNA Poly(A)
mRNA-ONLY Prokaryotic/Eukaryotic isolation Kit Terminator 5-Phosphate-Dependent
Exonuclease total RNA rRNA tRNATerminator 5-Phosphate-Dependent Exonuclease
5 3exonuclase 5-monophosphat RNA Total RNA rRNA 5
-monophosphat Terminator 5-Phosphate-Dependent Exonuclease 1
mRNA mRNA RT-PCRgene expression analysisNorthern blot
RNA
mRNA-ONLY Prokaryotic isolation Kit with poly(A)-Tailing Terminator 5-Phosphate-
Dependent Exonuclease Poly(A) polymerase mRNA 3 ATP A-tailing
reaction cDNA gene expression analysis
Ordering Information
mRNA-ONLY Prokaryotic mRNA Isolation Kit with poly(A)-Tailing 10 reactions MOT60510
mRNA-ONLY Prokaryotic isolation Kit 10 purifications MOP51010
24 purifications MOP51024
mRNA-ONLY Eukaryotic isolation Kit 10 purifications MOE51010
24 purifications MOE51024
: Terminator 5-Phosphate-Dependent Exonuclease, mRNA-ONLY Prokaryotic 10X Reaction Buffer, Ri-
boGuard RNase Inhibitor, mRNA-ONLY Stop Solution, 5 M LiCl Solution, RNase-Free Water.
E.coli total RNA(Lane1) 718nt RNA
mRNA-ONLY Prokaryotic/Eukaryotic isolation Kit
Terminator 5-Phosphate-Dependent Exonuclease
(Lane3) poly(A)-tailing mRNA (Lane4)
Lane5 total RNA and mRNA
Lane6 RNA mix poly(A)-tainking
Lane3 Lane5 Terminator 5-Phosphate-
Dependent Exonuclease total RNA
rRNA
mRNA Isolation P.24P.24
NucleoSpin RNA Clean-up/Clean-up XS
single reagent RNA
sample RT-PCR inhibitors
in vitro transcription NTP
RNA label reaction
RNA clean-up kit
RT-PCRDNA/RNA-based chip hybridizationin vitro transcriptionenzyme or
chemical labeling reaction
XS column 5ul RNA
Fig.1 NucleoSpin RNA Clean-up XS
100%
Lane 1: Crude RNA (100ul, 85ng/ul)
Lane 3: RNA Clean-up XS (20ul, 404ng/ul)
Lane 1 crude RNA NucleoSpin RNA
Clean-up XS major
band
>95% (Crude RNA 8500ng / Clean-up
RNA 8080ng)
B C
Fig.2 rat kidney total RNA amino-allyl-aRNA NucleoSpin RNA
Clean-up Q (A) Agilent Bioanalyzer
RNA NucleoSpin RNA Clean-up Q RNA
Q (A) NucleoSpin RNA Clean-up aRNA
array analysisB C
A MN
Q
RNA Ladder
RNA Clean-Up P.25P.25
RNA Clean-up
NucleoSpin RNA Clean-up NucleoSpin RNA Clean-up XS
Technology Silica membrane technology Silica membrane technology
Format Mini spin columns XS spin columns
Sample material Up to 200l Up to 300l
Fragment Size >200b >200b
Typical Recover 85 ~ 95% 85 ~ 95%
A260/280 1.9 - 2.1 1.9 - 2.1
Elution Volume 40 - 100 l 5 - 20l
Preparation Time 20min / 6 preps 20min / 6 preps
Binding Capacity 200 g 90 g
Product Information
Ordering Information
NucleoSpin RNA Clean-up 10 reactions 740948.10
50 reactions 740948.50
250 reactions 740948.250
NucleoSpin RNA Clean-up XS 10 reactions 740903.10
50 reactions 740903.50
250 reactions 740903.250
RNA Clean-Up P.26P.26
Fig.3 MN NucleoSpin RNA Clean-up
RNA NucleoSpin RNA Clean-up XS
MN NucleoSpin RNA Clean-up XS Q
3 (51x : 16x) NucleoSpin RNA Clean-
up RNA column 4.5x RNA
NucleoSpin TriPrep column RNADNAProtein
RNA DNA
Fig.1 NucleoSpin Triprep DNA
A260/280 1.81~1.94
1. Lamda DNA marker HindIII 2. HeLa
3. Mouse Liver 4. Zebrafish 5. garden cress root
6. garden cress leave 7.Yeast 8. Bacteria
Fig.2 NucleoSpin Triprep RNA Agilent
2100 RNAHeLa cell RIN=9.5mouse liver
RIN=9.1
1. Lamda DNA marker HindIII 2. HeLa
3. Mouse Liver 4. Zebrafish 5. garden cress root
6. garden cress leave 7.Bacteria 8. Yeast
Fig.3 NucleoSpin Triprep protein SDS-PAGE
1. Lamda DNA marker HindIII 2. HeLa 3. Mouse Liver
4. Zebrafish 5. garden cress root 6. garden cress leave
7.Yeast 8. Bacteria
P.27P.27
NucleoSpin Triprep 10 / 50 / 250 reactions 740966.10/.50/.250
Ordering Information
NucleoSpin RNA/Protein cultured cell RNA
DNase I RNA
RNA
gene expression analysissiRNA analysis
Fig.2 NucleoSpin RNA/Protein 106 HeLa cells
total RNA Agilent 2100 RNA RNA
quality
NucleoSpin RNA/Protein
Fig1. Inducible Knock-down of Hint1 in MCF-7 Tet-On cells
shRNA Hint1 silence Hint1 p53
A) 3 stable clonedempty vector (mock)vector with func-
tional shRNA (shRNA-Hint1 430/431) vector with inactive
shRNA (shRNA-Hint1 432/433) 1ug/ml dioxycycline
induction48 protein 50ug anti-Hint1
(anti-21) anti-b-actin (control) western bloting
B) & C) NucleoSpin RNA/Protein RNA
(B ) western bloting P53 b-actin
(control)RNA p53 primer b-actin primer (control)
RT-PCR C
Ordering Information
NucleoSpin RNA /Protein 10/50/250 reactions 740933.10/.50/.250
P.28P.28
NucleoSpin RNA/DNA buffer set NucleoSpin RNA IINuclsoSpin RNA XSNucleoSpin RNA Plant NucleoSpin RNA/
Protein kit
sample RNA DNA
PCRRT-PCRreal-time PCR
NucleoSpin RNA/DNA buffer set
DNA RNA HeLa cells
NucleoSpin RNA II NucleoSpin RNA/
DNA buffer set 5x106 HeLa cells DNA
RNA denature gel(A,C) TAE agarose
gel (B,D)
Table.1 NucleoSpin RNA/DNA buffer set
DNA RNA A260/280 ratio
ARF1 primer set PCRRT-
PCR NucleoSpin RNA/DNA buffer set
HeLa cell DNA RNA ARF1 primer
set gDNA intron gDNA PCR
RNA RT-PCR product 207bp
+ : positive control - : negative control
P.29P.29
NucleoSpin RNA /DNA buffer set 100 reactions 740944
Ordering Information
Bio-Genesis Technology Inc.
http://www.biogenesis.com.tw
[email protected] ;[email protected]
0800-211-667
02-26558877 037-687493
04-22602466 07-3422370 03-8463953
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