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Assessment of Beneficial Use Impairments for Benthos and Plankton in Wisconsin’s Lake Michigan AOCs Amanda Bell US Geological Survey, Middleton, WI In cooperation with the Wisconsin Department of Natural Resources Menomonee River 1 US Geological Survey

Evaluating Degraded Benthos and Plankton Communities-Bell, 2012

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Assessment tools to remove BUIs are inconsistent across the Great Lakes’ AOCs. This study assesses two BUIs, Degraded Benthos and Degraded Plankton Communities, using a design that compares AOCs to non-AOCs. Using non-AOC sites to determine if BUIs are still degraded, decisions can be made based on “control” sites.

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Page 1: Evaluating Degraded Benthos and Plankton Communities-Bell, 2012

Menomonee River 1

Assessment of Beneficial Use Impairments for

Benthos and Plankton in Wisconsin’s Lake Michigan AOCs

Amanda Bell

US Geological Survey, Middleton, WI

In cooperation with the

Wisconsin Department of Natural Resources

US Geological Survey

Page 2: Evaluating Degraded Benthos and Plankton Communities-Bell, 2012

Fox River 2

Background

• All of the AOCs along Wisconsin’s Lake Michigan shoreline have degraded benthos and three (not Menominee) have degraded plankton

• Consistent/comparable legacy community data for these sites doesn’t exist

• Stage 2 RAPs were due and TACs needed to identify projects that would address targets

• Wisconsin DNR and USGS designed a comparative study to assess plankton and benthos communities in AOCs and non-AOCS

US Geological Survey

Page 3: Evaluating Degraded Benthos and Plankton Communities-Bell, 2012

Milwaukee Harbor 3

Questions

• What is the current state of benthic invertebrate and plankton communities in Wisconsin's Lake Michigan AOCs?

• How do the benthos and plankton communities in these AOCs differ from rivers that are not considered AOCs?

• What community measures (i.e. IBI, richness, abundance, diversity) can be used as guides for determining benthos and plankton impairment in the AOCs?

US Geological Survey

Page 4: Evaluating Degraded Benthos and Plankton Communities-Bell, 2012

Sheboygan River 4

Sampling Design

• 10 Sites/3 times in 2012– 4 Wisconsin Lake Michigan AOCs

• Menominee River• Fox River/Green Bay (two river sites)• Sheboygan River (Replicate)• Milwaukee Estuary (two river sites, one harbor site)

– 6 Lake Michigan non-AOCs• Escanaba River• Oconto River• Anapee River (Algoma)• Kewaunee River• Manitowoc River (Replicate)• Root River (Racine)

US Geological Survey

Page 5: Evaluating Degraded Benthos and Plankton Communities-Bell, 2012

5US Geological Survey

Area of Concern

Non-AOC

Page 6: Evaluating Degraded Benthos and Plankton Communities-Bell, 2012

Oconto River 6

Sampling Scheme

• Plankton–Water Depth Profile– 63 µm Plankton Tow

• Benthos– Hester-Dendy Sampler– Ponar Dredge

US Geological Survey

Page 7: Evaluating Degraded Benthos and Plankton Communities-Bell, 2012

Menominee River 7

Water Depth Profile Sample

• Water sample (about 1 liter) from each meter of water depth down to 5 meters

• 3 times during growing season– June, July/August, September

• Identification/Enumeration at WSLH (soft algae) and by Paul Garrison, WDNR (diatoms)

• Chlorophyll a and Biomass analyzed at WSHL

US Geological Survey

Page 8: Evaluating Degraded Benthos and Plankton Communities-Bell, 2012

Milwaukee River 8

Plankton Tow Samples

• Vertical tow with 63-µm plankton net• 1-5 tows to obtain total of 5 meters of depth• Identification/Enumeration by Paul Garrison,

WDNR

US Geological Survey

Page 9: Evaluating Degraded Benthos and Plankton Communities-Bell, 2012

9

Hester Dendy Samples

• 3 per sampling location (15 total)• Deployed end of April– Retrieved June, redeployed– Retrieved July/August, redeployed– Retrieved September

• Identification/Enumeration at UW Superior

Menominee RiverUS Geological Survey

Page 10: Evaluating Degraded Benthos and Plankton Communities-Bell, 2012

Kewaunee River 10

Ponar Dredge Samples

• 3-5 dredges composited per sampling location (depending on substrate) near Hester Dendys

• 3 times during growing season– June, July/August, September

• Identification/Enumeration at UW Superior• Sediment size analysis at WSLH• Volatile on Ignition analysis by USGS

US Geological Survey

Page 11: Evaluating Degraded Benthos and Plankton Communities-Bell, 2012

Anapee River 11

Data Analysis

• Once data come back from lab (fall/winter 2012)–Macroinvertebrate Community assemblage

analysis• IBI Scores• Determine species composition differences between

sites• Multivariate statistics

US Geological Survey

Page 12: Evaluating Degraded Benthos and Plankton Communities-Bell, 2012

Milwaukee River 12

Data Analysis

• Site to site comparison– Benthos using WI Larger River IBI– Plankton using Shannon Diversity Index– For each season, if the AOC is less than 10% OR

one stand deviation (of all non-AOC sites) of selected comparison sites, then the seasonal sampled will be deemed degraded.

– If it is degraded two or more seasons, the benthos or plankton for the site is deemed degraded.

US Geological Survey

Page 13: Evaluating Degraded Benthos and Plankton Communities-Bell, 2012

Escanaba River 13

Data Analysis

• Overall comparison– Multivariate analyses designed for multiple sites with

similar multiple samples (seasons)• Tests if there is difference between groups (AOC vs. non-

AOC sites)• Tests if there is difference between multiple groups (sites or

seasons)

– Determine what species/metrics differ between AOC and non-AOC

– Deemed significantly different with a 90% confidence cut-off (p-value <10%)

US Geological Survey

Page 14: Evaluating Degraded Benthos and Plankton Communities-Bell, 2012

Kewaunee River 14

Future Questions

• Is there a significant difference in the plankton (and benthos) communities between each AOC and the non-AOCs?

• Which species, whether their present or absent, are indicative of systems that are NOT considered “degraded”?

US Geological Survey

Page 15: Evaluating Degraded Benthos and Plankton Communities-Bell, 2012

Manitowoc River 15

Questions?

US Geological Survey

Page 16: Evaluating Degraded Benthos and Plankton Communities-Bell, 2012

Root River 16

Contact Info

• Amanda Bell– US Geological Survey, Middleton, WI– [email protected]– 608-821-3882

US Geological Survey