Result “Integrin beta 1 appears in discrete puncta at the p of protrusions in migrang heart endocardial cells, similar to seen in cell cultures. There is non-specific staining in the blood cells.” F. Guzman - External Laboratory. Cell Line: Zebrafish embryos Method of validaon: Immunofluorescence (IF) Primary Anbody: STJ93731 An-Integrin β1 anbody Secondary Anbody Goat an-Rabbit IgG (H+L) Cross-Adsorbed Secondary Anbody, Alexa Fluor 568 Angen Retrieval Low concentraon of Pro- teinase K Diluon rao: 1:500 Anbody Customer Review: STJ93731 An-Integrin β1 anbody Anbody Specificity: Anbody Rang: Protocol Treatment of materials: Zebrafish embryos fixated with 4% paraformaldehyde. Angen retrieval: Low concentraon of Proteinase K. Blocking: 10% sheep serum, 1% BSA. Primary anbody probing: primary anbody was used at 1:500 concentraon, overnight at 4C. Membrane wash: 1X TBST wash for 3 mes. Secondary anbody probing: secondary anbody was used at 1:500 concentraon, overnight at 4C. Membrane wash: 1X TBST wash for 3 mes. Figure IIntegrin beta1 appears in discrete puncta (likely represenng focal adhesion complexes) at the p of protrusions in the leading migrang, heart endocardial cells, similar to seen in cell cultures. GFP used to mark membrane of migrang cells Overview and closeup of protrusions aached.

Immunofluorescence Antibody Customer Review Anti-Integrin β1 Antibody (STJ93731)

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Page 1: Immunofluorescence Antibody Customer Review Anti-Integrin β1 Antibody (STJ93731)

Result

“Integrin beta 1 appears in discrete puncta at the tip of protrusions in migrating heart endocardial cells, similar to seen in cell cultures. There is non-specific staining in the blood cells.” F. Guzman - External Laboratory.

Cell Line: Zebrafish embryos

Method of validation: Immunofluorescence (IF)

Primary Antibody: STJ93731 Anti-Integrin β1

antibody

Secondary Antibody Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 568

Antigen Retrieval Low concentration of Pro-

teinase K

Dilution ratio: 1:500

Antibody Customer Review: STJ93731 Anti-Integrin β1 antibody Antibody Specificity:

Antibody Rating:

Protocol

Treatment of materials: Zebrafish embryos fixated with 4% paraformaldehyde.

Antigen retrieval: Low concentration of Proteinase K.

Blocking: 10% sheep serum, 1% BSA.

Primary antibody probing: primary antibody was used at 1:500 concentration, overnight at 4C.

Membrane wash: 1X TBST wash for 3 times.

Secondary antibody probing: secondary antibody was used at 1:500 concentration, overnight at 4C.

Membrane wash: 1X TBST wash for 3 times.

Figure

IIntegrin beta1 appears in discrete puncta (likely representing focal adhesion complexes) at the tip of protrusions in the leading migrating, heart endocardial cells, similar to seen in cell cultures. GFP used to mark membrane of migrating cells Overview and closeup of protrusions attached.