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SmartEnzymes™ Genovis AB
IgG Proteases
IgG Glycosidases
Site-specific
endopeptidase
Site-specific
Antibody
conjugation
O-Glycan
elimination
- 2 -
IgG Proteases FabRICATOR® (IdeS) p.3
FragIT™ p.3
FabRICATOR® (IdeZ) p.4
FabULOUS® (SpeB) p.5
GingisKHAN® (KGP) p.6
FabALACTICA™ p.7
IgG Glycosidases GlycINATOR® (EndoS2) p.8
Immobilized GlycINATOR® p.8
IgGZERO® (EndoS) p.9
deGlycIT™ (Immobilized IgGZERO®) p.9
Site-specific endopeptidase GingisREX™ p.10
Lysyl Endopeptidase® p.20
Site-specific Antibody conjugation GlyCLICK™
p.11-p.12
O-Glycan elimination
OglyZOR™ p.13
OpeRATOR™ p.14
SialEXO™ p.15
GlycOCATCH™ O p.16
Bio-pharma research products
KANEKA KanCapA™ p.17
Ni-NTA Ni- p.18
p.19
Lysyl Endopeptidase p.20
Line up & Index
No.
A0-FR1-020
FabRICATOR® (IdeS)
FabRICATOR® (IdeS) Enzyme 8 96
2,000 Units 94,300
A0-FR1-050 5,000 Units 182,900
A0-FR1-250 5 x 5,000 Units 734,900
A0-FR1-008 8 x 100 Units 64,400
A0-FR1-096 96 x 100 Units 379,500
A0-FR6-010
FragITTM – Immobilized FabRICATOR® FabRICATOR® (IdeS)
2 x 0.5 mg 70,200
A0-FR6-025 5 x 0.5 mg 159,900
A0-FR6-050 10 x 0.5 mg 264,500
A0-FR6-100 1 - 10 mg 212,800
A0-FR6-1000 10 - 100 mg 634,800
A2-FR2-005
FragITTM Kit FabRICATOR® (IdeS)
0.5 mg 78,200
A2-FR2-025 5 x 0.5 mg 212,800
A2-FR2-100 10 mg 265,700
A2-FR2-1000 100 mg 797,000
A0-FR8-020 FabRICATOR® LE (Low Endotoxin)
2,000 Units 103,500
A0-FR8-050 5,000 Units 201,300
A0-FR4-060 FabRICATOR® Validation Kit 3 x 2,000 Units 282,900
A3-AF1-010 Anti-FabRICATOR® 0.1 mL 55,200
FabRICATOR®
Native IgG
Human IgG1 ..CPAPELLG / GPSVF..
Human IgG2 ..CPAPPVA / GPSVF..
Human IgG3 ..CPAPELLG / GPSVF..
Human IgG4 ..CPAPELLG / GPSVF..
Mouse IgG1 X
Mouse IgG2a ..CPAPNLLG / GPSVF..
Mouse IgG2b X
Mouse IgG3 ..CPPGNILG / GPSVF..
Rat IgG2b ..CPVPELLG / GPSVF..
Rhesus Monkey ..CPAPELLG / GPSVF..
Rabbit ..CPPPELLG / GPSVF..
Species & Subclasses
X = no digestion confirmed
Hu
Hu
Hu
Hu
Mo
Mo
Mo
Mo
ICATOR eS)
FabRICATOR® (IdeS) His
FabRICATOR® (IdeS) 0.02 - 0.05% (w/v)
: 1 10mM 137mM NaCl 2.7mM KCl pH7.4 37 30 1μg IgG 95%
FabRICATOR® (IdeS) Streptococcus pyogenes E. coli
- 3 -
FabRICATOR®
Native IgG
Human IgG1 ..CPAPELLG / GPSVF..
Human IgG2 ..CPAPPVA / GPSVF..
Human IgG3 ..CPAPELLG / GPSVF..
Human IgG4 ..CPAPELLG / GPSVF..
Mouse IgG1 X
Mouse IgG2a ..CPAPNLLG / GPSVF..
Mouse IgG2b X
Mouse IgG3 ..CPPGNILG / GPSVF..
Rat IgG2b ..CPVPELLG / GPSVF..
Rhesus Monkey ..CPAPELLG / GPSVF..
Rabbit ..CPPPELLG / GPSVF..
Species & Subclasses
No.
A0-FRZ-020
FabRICATOR® Z (IdeZ)
FabRICATOR® Z Enzyme 2,000 Units 94,300
A0-FZ6-010
FragITTM Z – Immobilized FabRICATOR® Z FabRICATOR® (IdeZ)
2 x 0.5 mg 70,200
A0-FZ6-025 5 x 0.5 mg 159,900
A0-FZ6-050 10 x 0.5 mg 264,500
A2-FZ2-005 FragITTM Z Kit FabRICATOR® (IdeZ)
0.5 mg 78,200
A2-FZ2-025 5 x 0.5 mg 212,800
FabRICATOR® Z (IdeZ) His
FabRICATOR® Z (IdeZ) 0.02 - 0.05% (w/v)
: 1 10mM 137mM NaCl 2.7mM KCl pH7.4 37 2 1μg IgG 95%
FabRICATOR® Z (IdeZ) Streptococcus equi ssp. zooepidemicusand E. coli
1. pH FabRICATOR®
pH pH
(PBS) 6.0 – 8.0 HEPES 7.0 – 8.0
Tris 7.0 – 8.0 6.0 – 7.0
MES 5.5 – 6.5 6.0
FabRICATOR® pH5.0 pH8.0
- 4 -
No.
A0-PU1-020
FabULUOS® (SpeB)
FabULOUS® Enzyme 2,000 Units 94,300
A1-PFK-020
FabULOUS® Fab kit FabULOUS® (SpeB) Fab L -Kappa
2,000 Units 159,900
1.
A) IgG1
B) FabULOUS Fab kit
C) CaptureSelectTM LC-Kappa column
Fab
Waters Acquity
UPLC® BEH300 C4 RP-HPLC (Agilent
1290)
2. FabULOUS Fab kit IgG
Fab SDS-PAGE
1 IgG1
2 IgG1
3 CaptureSelectTM
4 Fab
5
FabULUOS® (SpeB) His
FabULUOS® (SpeB)
: 1 FabULOUS® 1μg IgG
FabULUOS® (SpeB) Streptococcus pyogenes E. coli
pH
(PBS) 6.5 – 8.0
Tris 7.0 – 8.0
6.5 *
1. pH FabULOUS®
(mM) pH (mM) pH
DTT 1-5 6.5 - 8.0 50-100 6.5 - 8.0
TCEP 1-5 6.5 - 8.0 2- 50-100 6.5 - 8.0
50-100 6.5 - 8.0
2. FabULOUS®
- 5 -
1. GingisKHAN FabKit Fab
(A) IgG (B) GingisKHAN
(C) CH1 CaptureSelect Fc
(D) Fab
Supelco C4 bioshell A4 RP-UHPLC (Agilent
1260)
2. GingisKHAN Fab kit IgG1 Fab
SDS-PAGE
1 6 2 IgG 3
GingisKHAN Fab Fc 4 Fc GingisKHAN
5 Fab
: 1 0.1M Tris pH8.0 37 1 1μg IgG1 95%
Porphyromonas gingivalis
No.
B0-GKH-020
GingisKHAN® (KGP)
GingisKHAN® Enzyme 2,000 Units 94,300
B0-GFK-020
GingisKHAN® Fab kit GingisKHAN® (KGP) Fab CH1
2,000 units 212,800
- 6 -
150mM NaP 0-150mM
37 pH6.5-8 IgG1 E:S 1:10 150mM NaP pH7.0 37
IgdE
SDS-PAGE Fig. 1c RP-HPLC Fig. 1d HPLC Waters Acquity UPLC®
BEH300 C4, 1.7 μm, 2.1x100 mm / 60
Agilent 1260
FabALACTICATM (IgdE) His
FabALACTICATM (IgdE)
: 1 150mM Sodium phosphate pH7.0 37 16-18 1μg IgG1 90%
FabALACTICATM Streptococcus agalactiae E. coli
No.
A0-AG1-020 FabALACTICATM 2,000 units 110,400
- 7 -
GlyclNATOR® (EndoS2) His
GlyclNATOR® (EndoS2)
: 1 10mM Sodium phosphate 150mM pH7.4 37 30 1μg IgG 95%
GlyclNATOR® (EndoS2) Streptococcus pyogenes in E. coli .
GlycINATOR® LE GlycINATOR®
(< 0.2 EU) 2,000 unit/vial
No.
A0-GL1-020
GlyclNATOR® (EndoS2)
GlycINATOR® (EndoS2) 2,000 Units 94,300
A0-GL6-010
Immobilized GlycINATOR® GlycINATOR®
2 x 0.5 mg 70,200
A0-GL6-025 5 x 0.5 mg 159,900
A0-GL6-050 10 x 0.5 mg 264,500
A0-GL6-100 1-10 mg 212,800
A0-GL6-1000 10-100 mg 634,800
A0-GL8-020 GlycINATOR® LE 2,000 Units 103,500
Immobilized GlycINATOR® MidiSpin
Deglycosylates 1-10 mg
Immobilized GlycINATOR®
Deglycosylates 0.5 mg Immobilized GlycINATOR® Maxispin
Deglycosylates 10-100 mg
- 8 -
IgGZERO® (EndoS) His
IgGZERO® (EndoS)
: 1 10mM Sodium phosphate 150mM pH7.4 37 30 1μg IgG 95%
IgGZERO® (EndoS) Streptococcus pyogenes E. coli
IgGZERO® LE IgGZERO®
(< 0.2 EU) 2,000 unit/vial
deGlycITTM MidiSpin
Deglycosylates 1-10 mg
deGlycITTM MicroSpin,
Deglycosylates 0.5 mg deGlycITTM Maxispin
Deglycosylates 10-100 mg
No.
A0-IZ1-010
IgGZERO® (EndoS)
IgGZERO® Enzyme 1,000 Units 47,200
A0-IZ1-050 5,000 Units 190,900
A0-IZ6-010
deGlycITTM – Immobilized IgGZERO®
IgGZERO® (EndoS)
2 x 0.5 mg 70,200
A0-IZ6-025 5 x 0.5 mg 159,900
A0-IZ6-050 10 x 0.5 mg 264,500
A0-IZ6-100 1-10 mg 212,800
A0-IZ6-1000 10-100 mg 634,800
A0-IZ8-020 IgGZERO® LE 2,000 units 103,500
IgGZERO® (EndoS) GlyclNATOR® (EndoS2)
TM MicroSpin
ates 0.5
EndoS)
ITTM MidiSpin
ates 1-10 mg
ITTM Maxispin
ylates 10-100
® (EndoS2)
- 9 -
GingisREX® (Rgp)
GingisREX® (Rgp) TCEP ( pH) Cysteine ( pH) DTT
GingisREX® (Rgp) Porphyromonas gingivalis
No.
B0-GRX-005 GingisREX® (Rgp) GingisREXTM – Arginine Specific Protease 5 μg 102,400
1. β GingisREX Arg-C 37
1:20 w/w 20mM pH7.4 GingisREX
pH7.6 Arg-C
RP-HPLC 1
1. 1. GingisREX Arg-C β
6 β
2. GingisREX Arg-C Herceptin®
GingisREX Arg-C 1:50 w/w
37
RP-HPLC
- 10 -
GlyCLICKTM conjugation of Alexa Fluor® 488
(L1-F01-025) to 250 μg IgG
GlyCLICKTM conjugation of Biotin
(L1-A01-025) to 250 μg IgG
GlyCLICKTM conjugation of DFO
(L1-C01-025) to 250 μg IgG
GlyCLICKTM conjugation of Azide Activation
(L1-AZ1-025) to 250 μg IgG
No.
L1-F01-025 GlyCLICK conjugation of Alexa Fluor® 488 to 250 μg IgG 1 kit 193,200
L1-C01-025 GlyCLICK™ DFO, labeling of up to 250 µg IgG 1 kit 193,200
L1-A01-025 GlyCLICK™ Biotin, labeling of up to 250 µg IgG 1 kit 193,200
L1-AZ1-025 GlyCLICK™ Azide Activation of 250 μg IgG 1 kit 172,500
- 11 -
Application
1. Waters Acquity UPLC® BEH300 C4, 1.7 μm, 2.1x100 mm /
65 Agilent 1290
Herceptin®
A. FabRICATOR® F(ab')2 Fc/2
B. Alexa Fluor®488 FabRICATOR® F(ab')2 Fc/2
C. B.
Application LC/MS
GlyCLICKTM conjugation of Alexa Fluor® 488
GlyCLICKTM conjugation of Biotin
GlyCLICKTM conjugation of DFO
GlyCLICKTM conjugation of Azide Activation
- 12 -
2. LC/MS FabRICATOR Waters BEH300 C4
Bruker Impact ESI Q-TOF Agilent 1290 UPLC
MaxEnt
A. Fc/2 LC
B. GlycINATOR®
C. Fc/2 GlyCLICKTM 245 Da DIBO MMAE 1,781 Da
2,026 Da
OglyZOR™ SialEXO™ His
OglyZOR™ SialEXO™
: 1 OglyZOR™ 1 SialEXO™ 20mM Tris pH6.8 37 2 1μg TNFαR O-
90%
OglyZOR™ Streptococcus oralis E. coli SialEXO™ Akkermansia muciniphila E. coli
No.
G2-OG1-020 OglyZOR™ OglyZOR™
(OglyZOR™ Enzyme + SialEXO™) 2,000 units 182,900
- 13 -
OglyZOR™
OglyZOR™ TNFR
SialEXO™ 1 37 OglyZOR™ 3 O-
1. OglyZOR SialEXO
O-
OpeRATOR™ SialEXO™ His
OpeRATOR™ SialEXO™
: 1 OpeRATOR™ 1 SialEXO™ 20mM Tris pH6.8 37 2 1μg TNFαR 90%
OpeRATOR™ Akkermansia muciniphila E. coli SialEXO™ Akkermansia muciniphila E. coli
No.
G2-OP1-020 OpeRATOR™ OpeRATOR™
(OpeRATOR™ Enzyme + SialEXO™) 2,000 units 159,900
OpeRATOR™
- 14 -
SialEXO™ His
SialEXO™
1 SialEXO™ 20mM Tris pH6.8 37 2 1μg Fetuin 90%
SialEXO™ Akkermansia muciniphila E. coli
No.
G1-SM1-020 SialEXO™ SialEXO™ 2,000 units 113,900
OglyZOR™ OpeRATOR™
- 15 -
SialEXO™ SialEXO™ α2-3 α2-6 α2-8
SialEXO™
1. 3’-sialyllactose α2-3 6’-
sialyllactose α2-6 Colominic
acid α2-8
SialEXO™
1. H2686 H4062 H8390 β
IOB Glycodrosocin
SialEXO™ GlycOCATCH™
O-GalNAcGal
Glycodrosocin
RP-LC C18 Advance BioPeptide
Map 2.1x100 2.7µm [Agilent ] ESI-Q-
TOF Bruker Impact II mass spectrometer
O-GalNAcGal
No.
G3-OC6-002 GlycOCATCH™ 200 μg 136,900
- 16 -
- 17 -
111-01081
KANEKA KanCapA™ Prepacked Column
1 mL 22,000
117-01083 5 mL 60,000
115-01084 5 5 mL 240,000
Bio-pharma research products
114-01071
KANEKA KanCapA™
2 mL (net 1 mL) 10,000
110-01073 10 mL (net 5 mL) 40,000
118-01074 50 mL (net 25 mL) 150,000
- 18 -
146-09731 Ni-NTA Cartridge
1 (5 mL) 13,000
142-09733 1 5 (5 mL) 55,000
147-09761
Ni-NTA Agarose
2 mL Net 1 mL 4,500
143-09763 10 mL Net 5 mL 10,500
141-09764 100 mL Net 50 mL 62,000
149-09684
Ni-NTA Agarose HP
2 mL Net 1 mL 85,000
145-09681 10 mL Net 5 mL 13,000
141-09683 100 mL Net 50 mL 100,000
081-10381 1 L 12,000
297-73101 BCA 250 tests 15,000
637-25715
500 mL 8,700
635-25711 500 mL 10 70,000
- 19 -
125-05061 20 μg×5 19,500
125-02543 1 vial (2AU) 8,800
129-02541 1 vial (10AU) 41,000
127-06621 1 (5AU) 25,000
295-50201 50 19,500
202-15951 20 μg×5 17,900
299-58901 20 tests 19,000
293-57701 20 tests 34,000
®
19101.5 01J
https://labchem-wako.fujifilm.com/jp/index.html
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