View
224
Download
0
Category
Preview:
Citation preview
PAMC: Polar and Alpine Microbial Collec-tion
Yung Mi Lee1, Cheng-Dae Choe2, Jeong-Han Yim1, Hong Kum Lee1, and Soon Gyu Hong1
1 Korea Polar Research Institute 2 BRN Science Co.
Needs for Database
▣ Management and sharing of bio-resources from polar areas
▣ Sharing biodiversity information with national and international organization
www. iobis.org
Culture Collections
No. of Culture collections No. of Cultures
Total 585 1,725,498
755,818 of them are Bacteria.505,521 of them are Fungi....
www.wfcc.info
v
PAMC: Polar and Alpine Microbial Collection
▣ pamc.kopri.re.kr
▣ Culture Collection to maintain and distribute microorganisms originated from polar areas
▣ Approximately 500 strains are available for delivery and the number will increase gradually
Polar and Alpine Microbial Col-lection
Polar and Alpine Microbial Collection
About PAMC
Data Management of PAMC
PAMCPAMC
CuratorsCurators
Public databasesPublic databases
Public usersPublic users
Search
Datadownload
Public users Contributors
Public users Contributors
Deposit of resources
Delivery of resources
• Long-term preservation of bio-resources• Identification• Characterization• Data updates
• Metadata upload (AMD)• Original data transfer (EBA, SCAR-Marbin etc)
• Data download (GenBank Taxonomy etc)
Strains
▣ Microbial cultures Bacteria: 6,400
strains
Fungi: 300 strains
Freezing using Glycerol (-180C) and DMSO (-80C)
Freeze-dry (4C)
▣ Preservation
Preservation of strains
84%
16%
Abiotic sample
Biotic sample
Phylum composition of identified strains
Gammaproteobacteria,597
Actinobacteria, 188
Bacteroidetes,191
Firmicutes, 265
Betaproteobacteria,49 Alphaproteobacteria,50
Habitats of strains
Summary of PAMC Strains
Locality distribution of strains
24%
67%
10%
Antarctica
Arctic
Other
Kara sea (40%) Svalbard (21%) Barents sea (3%) Russia (3%))
King George Island (23%) Adeli Island (0.5%)
Austria (Alps) (8%) Mongol (1%) Okhotsk sea (1%)
Sediment 43.70%Soil 20.50%Water 9.70%Cryoconite 8.50%Rock 1.10%Glacier/Snow 0.30%
Biofilm 8.50%Algae 3.60%Lichen 1.30%Debris (animal/plant)
1.20%
Animal 0.80%Moss 0.80%
Composition of Identified Strains
Actinobacte-ria 16 Genera 188 Strains
Bac-teroidetes 25 Genera 191 Strains
Firmicutes 11 Genera 265 Strains
Alphaproteobacte-ria 16 Genera 50 Strains
Betaproteobacte-ria 4 Genera 49 Strains
Gammaproteobac-teria 18 Genera 597 Strains
Physiological Characteristics
▲ Growth on the 96 well plate
▲ Formation of hydrolytic zone on the 96 well plate
Growth Temperature
No.
of
Str
ain
sN
o.
of
Str
ain
s
4℃ 10℃ 15℃ 20℃ 25℃ 30℃ 37℃0
500
1000
1500
2000
2500
3000
Score 1Score 2Score 3Score 4
ProteaseLipase 10℃ 20℃0
100
200
300
400
500
Exopolysaccha-ride
10℃ 20℃ 10℃ 20℃0
200
400
600
800
1000
Score 1Score 2Score 3Score 4
Sample Database
Strain Database
①
Pseudoalteromonas
②
④
v
v
v
v
③
PAMC - Searching
Polar and Alpine Microbial Collection
Polar and Alpine Microbial Collection
PAMC – Advanced Searching
Searching Keywords in PAMC
Searching for microbial strains using various keywords such as taxonomy, ge-ography, habitats and physiological characteristics are available in PAMC.
Taxonomy Geography
Habitats Characteristics
Polar and Alpine Microbial Collection
Vision of PAMC
▣ To maintain strains for the sustainable use
▣ To supply authentic strains for polar sciences
▣ To expand the service materials: fungal culture, gDNA, metabo-lite extract, etc
▣ PAMC website will be available on 1 October
Thank you!
Recommended