View
33
Download
1
Category
Preview:
DESCRIPTION
pcr
Citation preview
POLIMERASE CHAIN REACTION (PCR)
POLIMERASE CHAIN REACTION (PCR)
• Kary Mullis (1984)• PENGGANDAAN FRAGMEN SPESIFIK DNA
SECARA INVITRO JUTAAN KOPI FRAGMEN DNA SECARA CEPAT DAN AKURAT
REPLIKASI
PCR
Basic Components
• TEMPLATE ATAU TARGET (FRAGMEN GEN YANG DIINGINKAN)
• DNA ATAU RNA
• dNTPs
• PRIMER
– OLIGONUKLETIDA PENDEK (15-30 BP)
– TERIKAT DENGAN SEKUENS SPESIFIK PADA TEMPLATE
• ENZIM
– HEAT-STABILE DNA POLIMERASE –Taq DNA POLIMERASE
– REVERSE TRANSCRIPTASE
Step 1 : Denaturation (separation of double-stranded DNA) by Heat (95° C)
Step 2 : Primer Anneals (50 - 65° C)
sstep 3 Primer Extension by Taq Polymerase (72° C)
End of the 1st PCR Cycle Results in 2 Copies of Target Sequence
CYCLE NUMBER AND AMPLICONS
VARIASI GENETIK (POLIMORFISME)
SINGLE NUCLEOTIDE POLYMORPHISMS (SNP) SPESIFIC REPEATED SEQUENCE (TANDEM REPEAT)
Recommended