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HOW DID OUR ANTIBODY PERFORM?
ANTIBODY CUSTOMER REVIEW: Laminin a-1 Polyclonal Antibody (STJ93888)
What was used?Primary antibody: STJ93888 Laminin a-1
Provider: St John's Laboratory Ltd.
Dilution ratio: 1:50
Application: Immunofluorescence staining
Materials for validation: Decellularised cornea
Click for product data sheet PDF
What Was The Protocol?Treatment of Materials Tissue was sectioned at a 7 um thickness, and then fixed with 4% parafolmaldehyde and
permeabilized with 1% Triton X-100.
Fixation 4 % parafolmadehyde at room temperature for 20 minutes.
Permeabilization 1% Triton X-100 at room temperature for 5 minutes.
Blocking Blocking buffer (PBS with BSA and goat serum) for 1 hour.
Primary Antibody Probing Primary antibody was added to the solution of blocking buffer (PBS with BSA and goat serum), at 4˚C overnight.
Sample Wash The sample was washed with PBS with BSA and goat serum 5 times, each for 5 minutes.
Secondary Antibody Probing Secondary antibody was added to the solution of blocking buffer (PBS with BSA and goat serum) with a 1:100 dilution ratio, for 1 hour.
Sample Wash The sample was washed with PBS with BSA and goat serum 7 times, each for 5 minutes.
Visualization Nikon Axiovert microscope.
What was the protocol?
No. Antigen Primary antibody
Primary antibody
dilution ratio
Secondaryantibody
dilution ratio
1 Decellularised cornea
STJ93888 Laminin a-1
antibody1:50 1:00
What were the results?
What did the customer think?
Antibody Specificity:
Antibody RPPA Rating:
Testing results were provided independently by F. Abidin at Newcastle University. They stated “Visible laminin staining was observed from this tissue section.”
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