Bulletin of the Livestock Research Institute, Toyama Prefectural Agricultural, Forestry and Fisheries Research Center

February 2011

Livestock Research Institute, Toyama Prefectural Agricultural, Forestry and Fisheries Research Center

Toyama, Toyama, Japan

富山県農林水産総合技術センター畜産研究所研究報告　　

第一号（通巻

二十号）

二〇一一年二月

Toyama Prefectural Agricultural, Forestry and Fisheries Research Center

Livestock Research Institute

1 Chisato-Maeyama, uchu-machi Toyama-shi,Toyama

1 2009

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富山県農林水産総合技術センター畜産研究所研究報告　第１号（通巻 第20号）�

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(2003)

J.Trad.Med.20:30-37

(2010 12 14 )

富山県農林水産総合技術センター畜産研究所研究報告　第１号（通巻 第20号）

(2003)

J.Trad.Med.20:30-37

(2010 12 14 )

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To prevent infectious diseases such as diarrhea , the herb medicine remnants were given to Japanese

Black calves. In herb medicine remnants group, calves were given 100g herb medicine remnants a day with

concentrate. We measured the immune globulin G(IgG) ,IgM ,IgA etc in serum before and 0, 0.5, 1.0, 2.0

and 3.0 months after administration of remnants. As a result, the effects such as the increase of the immune

globulin following administration of remnants were not provided.

Key words herb medicine remnants Japanese Black calves immune globulin

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1 )Donnay I, Langendonckt AV, Auquier P, Grisart B, Vansteenbrugge A, Massip A and Dessy F (1997) Effect of co-culture and embryo number on the in vitro development of bovine embryos. Theriogenology 47:1549-1561. 2 ) 2006

2132-33

3 )Fujita T, Umeki H, Shimura H, Kugumiya K, and Shiga K (2006) Effect of group culture and embryo-culture conditioned medium on development of bovine embryos. J. Reprod. Dev. 52:137-142. 4 )

2006CR1aa

2264-65.

(2010 12 15 )

富山県農林水産総合技術センター畜産研究所研究報告　第１号（通巻 第20号）�

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富山県農林水産総合技術センター畜産研究所研究報告　第１号（通巻 第20号）�0

生米ぬかや生稲わらサイレージを混合した黒毛和種去勢牛向け発酵ＴＭＲの品質と採食性 ��

富山県農林水産総合技術センター畜産研究所研究報告　第１号（通巻 第20号）��

生米ぬかや生稲わらサイレージを混合した黒毛和種去勢牛向け発酵ＴＭＲの品質と採食性 ��

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9 1220

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900%

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パーコールを含む選別液によるウシ受精卵の品質評価

BSA

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5 5 SOF7

9 1220

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富山県農林水産総合技術センター畜産研究所研究報告　第１号（通巻 第20号）

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Farin 1994

Crosier 2000 2001

Shamsuddin 1992

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12

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12 1410

Croier AE, Farin PW, Dykstra MJ, Alexander JE, Farin CE. Ultrastructural morphometry of bovine compact morulae produced in vivo or in vitro. Biol Reprod 2000; 62: 1459-1465.

Croier AE, Farin PW, Dykstra MJ, Alexander JE, Farin CE. Ultrastructural morphometry of bovine blastocysts produced in vivo or in vitro. Biol Reprod 2001; 64: 1375-1385.

Farin PW, Britt JH, Shaw DW, Slenning BD. Agreement among evaluators of bovine embryos produced in vivo or in vitro. Theriogenology 1995; 44: 339-349.

Shamsuddin M, Larsson B, Gastafsson H, Gustari S, Bartolome J. Comparative morphological evaluation of in vivo and in vitro produced bovine embryos. Proc 12th

Inter Congr Anim Reprod 1992; 3: 1333-1335. Shiku H, Shiraishi T, Ohya H, Matsue T,

Abe H, Hoshi H, Kobayashi M. Oxygen consumption of single bovine embryos probed by scanning electrochemical microscopy. Anal Chem 2001; 73: 3751-3758.

Stojkovic M, Machado SA, Stojkovic P, Zakhartchenko V, Hutzler P, Goncalves PB, Wolf E. Mitochondrial distribution and adenosine triphosphate content of bovine oocytes before and after in vitro maturation: correlation with morphological criteria and developmental capacity after in vitro fertilization and culture. Biol Reprod 2001; 64: 904-909.

パーコールを含む選別液によるウシ受精卵の品質評価 ��

Takahashi Y, First NL. In vitro development of bovine one-cell embryos: influence of glucose, lactate, pyruvate, amino acids and vitamins. Theriogenology 1992; 37: 963-978.

Yotsushima K, Shimizu M, Kon H, Izaike Y. A simple method for selection of cumulus-oocyte complexes from bovine ovaries by sedimentation with Percoll. J Reprod Dev 2007; 53: 971-976.

(2010 12 28 )

富山県農林水産総合技術センター畜産研究所研究報告　第１号（通巻 第20号）��

The objective of this study was to clarify relationships between sedimental fractions with Percoll of bovine embryos and their developmental competence. Bovine embryos produced in vivo were collected from superovulated Japanese Black cows. In vitro produced embryos were obtained from cumulus-oocyte complexes aspirated from ovaries of slaughtered cows. In vitro and in vivo embryos appearing from compact morulae to expand blastocyst stage were sedimented with Percoll solution and determined those sedimental fractions. The frequency of in vivo produced embryos collected from 12% and 14% Percoll fractions were higher than those of in vitro produced embryos. Following with the embryo development, they were selected to lower ratio Percoll fractions. Compact morulae-expand blastocyst stage embryos collected from 12-14% Percoll fractions showed higher developmental competence and cryo-tolerance than embryos collected from 10% Percoll fraction. These results suggest that the criteria of embryo quality by sedimentation with Percoll solutions may be effective.

Key words bovine embryos Percoll quality evaluation

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イネ穀実サイレージの物理的または化学的処理が消化性に及ぼす影響 ��

富山県農林水産総合技術センター畜産研究所研究報告　第１号（通巻 第20号）��

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富山県農林水産総合技術センター畜産研究所研究報告　第１号（通巻 第20号）��

イネ穀実サイレージの実用的調製方法 ��

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富山県農林水産総合技術センター畜産研究所研究報告　第１号（通巻 第20号）��

既報号目次（富山県畜産試験場研究報告および富山県農業技術センター研究報告） ��

富山県農林水産総合技術センター畜産研究所研究報告　第１号（通巻 第20号）�0

既報号目次（富山県畜産試験場研究報告および富山県農業技術センター研究報告） ��

富山県農林水産総合技術センター畜産研究所研究報告　第１号（通巻 第20号）��

Toyama Prefectural Agricultural, Forestry and Fisheries Research Center

Livestock Research Institute

1 Chisato-Maeyama, uchu-machi Toyama-shi,Toyama

Bulletin of the Livestock Research Institute, Toyama Prefectural Agricultural, Forestry and Fisheries Research Center

February 2011

Livestock Research Institute, Toyama Prefectural Agricultural, Forestry and Fisheries Research Center

Toyama, Toyama, Japan

富山県農林水産総合技術センター畜産研究所研究報告　　

第一号（通巻

二十号）

二〇一一年二月