Θερμοσταθερότητα πρωτεϊνών μέσα στην εξέλιξη

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  • :

    : (DHFR) - - ...

  • !

    Life can be found in arcticand antarctic waters holding temperatures below theordinary freezing point of water, to superheated water inhot springs and in deep ocean smokers, where someorganisms can survive temperatures up to 121C*

  • (Tmin), (Topt), (Tmax).

    , , .

    1. C.

  • -5C 113C. .

    2. / 1.

    *

  • 3. Hydrothermal vents. Black smokers. : 80C. 60C. 4. Yellowstone National Park, USA.

  • : Archaea, Bacteria Eucarya 5. rRNA .

  • , Moritella profunda, Escherichia coli K-12 Thermotoga maritima.

    2.

    Moritella profundaEscherichia coli K-12Thermotoga maritimaTmin2C 11.33C60 CTopt2C40.85C80CTmax12 C46.54C90 C

  • ( , )

  • ( ) ( ) !!! 6.

    . *

  • . . ( ) ( )

  • DHFR (EC 1.5.1.3) , . DNA - (NADPH) , (MTX).

    7. DHFR NADPH ( ) MTX ( ), PDB ID 3DAU 1.5

  • !!!

    3.

    , -, , , , (. )

    *

  • DHFR , () .

  • . DHFRs : : :

  • , , , . . .

  • . / . . DHFR

    .

  • DHFR , . 3 DHFR NADPH Moritella profunda, o o Escherichia coli K-12 o o Thermotoga maritima. >35%. :

    1. , DHFR Clustal Omega (McWilliam et al., 2013). .

  • 1. DHFR

    DHFR PDB3IA43DAU1D1G PDB412 112/162159168 38C 53C 83C (3)27187 26251 29006 (2)5919 5839 6658 ,0.740.730.69 ()13.78 13.49 13.07

  • () 2. () () R2=0.99

  • DHFR 3. Open-Source Pymol 1.6.x MTX ( , sticks) NDP ( , sticks) () , () () DHFR , () () () (PDB ID 3IA4)(PDB ID 3DAU)(PDB ID 1D1G)

  • DHFR . DHFR

    2. 2 (2)()() (3)() () () 2 DHFR 4.

    3V: Voss Volume Voxelator (3) DHFR (2)Channel_1Channel_2Channel_1Channel_2(PDB ID 3IA4)1048061741151 (PDB ID 3DAU)21615403141437(PDB ID 1D1G)50718987221637

  • 3. (2) WHAT IF Server 4. (2) WHAT IF Server

    / / / ALA 8.18.97.5CYS 6.16.60ASP 24.023.79.3GLU 22.023.127.5PHE 2.310.99.5GLY 6.35.89.0HIS 24.518.60ILE 4.13.16.6LYS 29.725.131.8LEU 6.14.69.8MET 7.512.317.3ASN 16.017.714.9PRO 11.619.917.3GLN 23.618.70ARG 24.428.430.2SER 6.512.715.0THR 10.58.45. 5VAL 12.28.36.5TRP 14.38.310.2TYR 8.3525.9

    DHFR3IA43DAU1D1G/ 1987.02181.52493.6 / 12.313.715.6/ 48.534.735.2/ 54.7117.0164.9

  • DHFR DHFR - ,

    ()()() 5. () - () - ()

  • (1)

    , DHFRs DHFR DHFR

    5. 3

    6. /

    A9.3%8.2%3%V6.8%6.9%11.3%F2.5%3.8%9.5%P5%6.3%4.8%L10.6%6.9%8.9%I6.8%7.6%4.8%R5.6%5.7%7.7%D5.6%8.2%3%E8.1%7.6%10.1%S3.1%5.7%6.6%T5.6%3.8%5.4%C0.6%1.3%0.0%N5.6%3.8%4.2%Q3.7%2.5%0.0%H2.5%3.2%0.6%K3.1%3.8%7.7%M3.1%3.2%1.8%Y4.4%2.5%2.4%W1.9%3.1%0.6%G6.2%6.3%7.7%

  • (2) (3) DHFR DHFR

    DHFR DHFR

    6. 7. /

  • (4) 6.

    0-7100%000

    7-1461.8%20.6%13.2%4.4%61.2%16.4%16.4%6%63.9%13.1%14.8%8.2%14-2154.2%18.1%8.3%19.4%50%14.9%12.2%23%50%15.4%14.1%20.5%

    (100% - ) 0-8.5 0-8.5 0-9

  • 7. (6) ()

    ()() 8. () - () -

    -3IA43DAU1D1G% : :R 4.3

    6.8

    8.6

    K :C , H, PC4.300H02.30P2.24.52.9

  • Open-Source Pymol 1.6.x (DeLano, 2002) , - 3 3 .

    9. 3 (), () ()()()()

  • RMSD: 1,421RMSD: 2,471RMSD: 0,531 DHFR DHFR

    10. () - () - () -()()()

  • , DHFR 11. - - DHFR () ribbon() cartoon() () DHFR

  • 8. - 3

    (3IA4) (3DAU) (1D1G) 129135146(133 )3IA4_ WATER 3DAU_ WATER 1D1G _ WATER524392249 53

    55

    72(236 )

    104

    121

    127

    12145

  • . DHFR DHFR DHFR DHFR DHFR 16S rRNA

    12. Neighbour Joining tree Using BLOSUM62 3

    *

  • 1: DHFR ( , -) . , . DHFR ( ). , DHFR . -, (, ). .

  • 2: DHFR . ( ) DHFR ( ). DHFR - - . .

  • (DHFR) housekeeping . (). 16S rRNA DHFR. ( ). , .

  • DHFRs DHFR Thermotoga maritima . (/ ) DHFR.

  • ... !

    Life can be found in arcticand antarctic waters holding temperatures below theordinary freezing point of water, to superheated water inhot springs and in deep ocean smokers, where someorganisms can survive temperatures up to 121C* * . * *

    *