AMR ESBLESBL

ESBLESBL

AMRAMR ESBL

A

ESBLESBL

AMR

ESBLESBL CTX-M-1, M-2, M-9, M-8 group plasmid

AmpC pAmpC; MOX, CMY-2, DHA, ACC, EBC, FOX PCRPCR ESBL

variant ESBL/pAmpCESBL

PCR-based open reading frame typing (POT) plasmid replicon typing phylogenetic group typing B2-ST131

1-A ESBL/pAmpC 2016 3 8

256 25632 12.5% ESBL pAmpC CMY-2 CIT

3 E. coli 2 Serratia liquefaciens 1 DHA 1 E. coliTable 1

1-B ESBL/pAmpC 2015 4 2017 12 116

83 33 116 ESBL10 8.6% 9 1 pAmpC 2 1.7% 11 pAmpC 2 CMY-2 Table 2

2 ESBL

32 ESBL CTX-M-14 14 CTX-M-27 7 CTX-M-554 CTX-M-15 3 CTX-M-79 3 CTX-M-65 110 CTX-M-14 CTX-M-1 CTX-M-44 2 CTX-M-65

CTX-M-15 CTX-M-79 CTX-M-8 1 Table 1

3

CP TCSM KM

ST NA NFLX GMFOM CPFX IPM 11ESBL CP 3.1%(1/32) TC 34.4%(11/32)

SM 37.5%(12/32) KM 6.3%(2/32) ST 31.3%(10/32), NA 75%(24/32) NFLX

50%(16/32), GM 21.9%(7/32) FOM 0%(0/32) CPFX 50%(16/32) IPM 0%(0/32)ESBL TC NA SM 50%(5/10)

4

Clermont 1) 30B2 15 50% D 13 43.3% A 2 6.7%

ESBL 9 A 5 55.6% B1D 2 22.2% POT

30 POT 26 POT2 POT 49-58-83 4 ST131

Table 3 8 POTPOT 1 10-1-0 2 POT 1

2 sample C D 2 CTX-M-44

5 ST131 H30

Johnson 2) ST131-specific PCR ESBL30 10 ST131 Table 3 ST13110 Colpan 3) ST131 H30

allele 30 of fimH H30H30R ST131 10 ESBL CTX-M-276 CTX-M-14 2 CTX-M-15 CTX-M-79 1

ST131 10 7 H30RST131

6 replicon typing ESBL

32 22 IncF FIA FIB8 IncI 5 ST131

10 IncF H30R 7FIA Table 3 ESBL 5 IncI1 3

IncF 3 Table 2

ESBL 12.5% 8.6%

4)

ESBLCTX-M-14 CTX-M-27 CTX-M-8

CTX-M-14 CTX-M-15CTX-M-65 CTX-M-79 4 POTPOT

ST131B2 D 93.3%

commensal A B1 77.8%ESBL

replicon type IncFIB IncI1 typeESBL ST131

4) ST131IncI1

CTX-M-8 1 Table 2 sample ICTX-M-8 IncI1

5) ESBL

Clermont O, Bonacorsi S, Bingen E. (2000) Rapid and simple determination of the Escherichia coli phylogenetic group. Appl Environ Microbiol. 666, 4555-4558. Johnson JR, Clermont O, Johnston B, et al (2014) Rapid and specific detection, molecular epidemiology, and experimental virulence of the O16 subgroup within Escherichia coli sequence type 131. J Clin Microbiol. 552,1358-1365. Colpan A, Johnston B, Porter S, et al (2013) Escherichia coli sequence type 131 (ST131) subclone H30 as an emergent multidrug-resistant pathogen among US veterans. Clin Infect Dis. 557,1256-1265. Kawamura K, Sugawara T, Matsuo N, et al (2017) Spread of CTX-Type Extended-Spectrum -Lactamase-Producing Escherichia coli Isolates of Epidemic Clone B2-O25-ST131 Among Dogs and Cats in Japan. Microb Drug Resist. 23,1059-1066. Norizuki C, Wachino JI, Suzuki M, et al (2017) Specific blaCTX-M-8/IncI1 Plasmid Transfer among Genetically Diverse Escherichia coli Isolates between Humans and Chickens. Antimicrob Agents Chemother. 224,61(6).