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The aim of this study was to investigate the mechanism of bone remodeling while focusing on the
integrity of osteocyte network. Both an in vitro experimental system that could apply local mechanical
damage to the cultured osteocytes and an animal model that could give a fatigue microcrack to a mouse
tibia were developed. A large deformation of the culture substrate or bone matrix damaged the osteocyte
network, and apoptotic cells were derived around the necrotic osteocytes. This apoptotic cell induction
would be a key response for initiating bone remodeling, by which a damaged bone matrix could be
repaired locally and appositional bone formation would be occurred to compensate the drop of bone
strength.
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