2. 2012910 1995 CNRS CEVA Amadeite - MTX ++ ULVANS BRITTANY 910
.Herv Balusson 1 3. 2012910 : : Catherine Boyen - -- , -
Bioge-nouest8.309.00 . - : - 9.10 p.11Christine Bodeau-Bellion -
-Le Relecq Kerhuon, 9.30Eric Deslandes - , p.179.50Philippe Potin -
- -- IDEALG p.3010.20Mirjaml Czjzek - ---11.00 11.30Nathalie
Bourgougnon - - , p.372 4. 2012910 () 12.00p.47 Henri Salmon -
INRA- 12.30 Philippe Bougnoux - --CHU p.58 13.00 : : Christine
Bodeau-Bellion - 14.15 Eliana Henriquez Flores - - - -p.68 , :
14.30p.74 Alexis Rannou - - , : 14.50 Maria Hayes - NutraMara-
Teagasc Ashtown - p.87 Christine Le Tennier -, p.101 15.25p.104
Bruno Daridon - - PRP , 16.00 () 16.15p.119 Adeline Picot - - , :
16.40p.128 Simon Davies - () - 17.10 . - 17.30 19.00 - 3 5. 2012910
Mr Herv Balusson - 50Catherine Boyen - -- , -BiogenouestChristine
Bodeau-Bellion - Le Relecq Kerhuon, Eric Deslandes , Philippe Potin
- -- IDEALG Philippe Potin 1992 , NRCHalifax(NS Canada) CNRSRoscoff
P. Potins(70 ) Gomar 4Maritime Cluster Ple Mer Bretagne( )
IDEALGFrench Stimuli Program Investissements dAvenir 10 . Philippe
POTIN, Station Biologique de Roscoff, BP 74 - 29680 Roscoff,
Tel.33-2. 98.29.23.75, Fax.33-2. 98.29.23.85, Mail:
[email protected] 6. 2012910Mirjaml Czjzek --- Mirjam Czjzek
LCMB 199210 CNRS Bernard Henrissat -CAZymes2005 CBMsNathalie
Bourgougnon - , Nathalie Bourgougnon 2001 8 55 FP4 MAST IIIFP5
AVINSI- FP6 Valbiomar FP7 Biotecmar OSEOULVANS-MAE ASEM-DUO
BioChi-MarSEAProRAQ Quebec Nathalie BourgougnonJL Mou-getV.
StigerT. RenaultIFREMER, LP BergIFREMERM, TtouanPr. H. Riadi
TerengganuISMER (Qubec, Dr. R. Tremblay) UEBUniversit europenne de
Bretagne www.ueb.eu . 5 7. 2012910 Henri Salmon INRA- Henri
SalmonINRA INRA 61984INRA (Nouzilly) IgA IgA IgA IgA .Philippe
Bougnoux --CHU Philippe Bougnoux 3 Henry S. Kaplan 1069 , () -3 .
Address: INSERM U1069, Henry S. Kaplan Cancer Centre, University
Hospital Bretonneau, 37044 Tours, France Telephone: +33 (0) 2 4747
8261 Email: [email protected] Web site:
www.n2c.univ-tours.fr6 8. 2012910Eliana Henriquez Flores - -
-,1988-1995 20042008SAGAlexis Rannou - , Ingnieur Agricole (ISAB)
1991Ingnieur dtudes ARD (Agro industrie Recherche et
dveloppement)en charge de la slection varitale de la betterave
biotechnologie pour lafabrication dacide galacturonique.1994
Responsable du pilote industriel ARD miseau point de
tension-ac-tifs verts (Uronate de sodium et Alkyl
polypentosides)1997 Directeur technique SOLIANCE & dveloppement
industriel Amadte avec OLMIX2000 Formation IFG CGDPME (Gestion des
entreprises)2002 Directeur Gnral Adjoint en charge de la production
et du compte lOral2007 DGA en charge de linnovation (8 Brevets) 20
bx produits et du marketing strat-gique2012 DGA en charge de
linnovation Soliance et Wheat Olo7 9. 2012910 Maria Hayes
NutraMara- Teagasc Ashtown - NutraMara& -, ACE-I, PAF-AH, PEP
Short Biography Hayes Moorepark NutraMara2Walsh Ciaran
FitzgeraldMichelle Tierney Selected publications Fitzgerald, C.,
Gallagher, E., Tasdemir, D., Hayes, M., (2011) DOI:
10.1021/jf201114d Di Bernardini, R., Harnedy, P., Bolton, D.,
Kerry, J., O Neill, E., Mullen, A. M., Hayes, M., (2011), 124,
1296-1307 Tierney, M. S., Croft, A. K., Hayes, M., (2010) Botanica
Marina, 53 (2010), 387-408. Hayes, M., Carney, B., Slater, J.,
Bruck, W., (2008), B3, 7, 871-877. Hayes, M., Barrett, E., OConnor,
P., Gardiner, G., Fitzgerald, G., Hill, C., Stanton C., Ross R.P.
(2007), Salivaricin P: Appl Environ Microbiol. 73, 11, 3719-3723.8
10. 2012910Christine Le Tennier -,Christine Le Tennier 20Christine
Le Tennier 20221986Christine Le TennierSnc Globexport 1993Sarl
Globe Export-10 , , Christine Le tennier 213-15 : 20%20122.Bruno
Daridon - PRP , Bruno
Daridon,5120073PRP1984198819931997Prabil199720042007 Novasep
Brabois9 11. 2012910 Adeline Picot - , 2010Adeline
PicotVegenovElici-tra Simon Davies () - 10 12. : Christine BODEAU :
Jean DUGOUJON, Pierre ARZEL, Jean-Yves FLOCH, 1 19603 21Bretalgue2
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:13
MarnagesdansleMonde.-Source:SHOMPluspetits=verts/Plusgrands=rouges4
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2012910 - 2012910 9 13 15. 10 - 2012910 11 - 2012910 17 191955 19 /
1960 1980 14 - 2012910 12 16. 13 - 2012910 14 - 2012910() () () -
2012910 1515 17. : , ... - 2012910AlguesetMer16 BordBord -
2012910AlguesdeBretagne 17 16 - 201291018 18. : , Philippe POTIN -
Team Algal DefensesCNRS-UPMC UMR 7139 - Marine Plants &
Biomolecules 1Roscoff : Olmix Algae Symposium -Sept.10th,2012 2
Floch, J.-Y. (1964). Distribution verticale et cologie des algues
marines sur les ctes Bretonnes. Penn ar Bed 4(37)Olmix Algae
Symposium -Sept.10th,2012317 19. Olmix Algae Symposium
-Sept.10th,20124 Olmix Algae Symposium -Sept.10th,20125 2.724
billion years ago as far back as 3.450 billion years
agoModernstromatolitesinSharkBay,WesternAustraliahttp://en.wikipedia.org/wiki/Stromatolite18
Olmix Algae Symposium -Sept.10th,20126 20. . , , , (BGA),,.Olmix
Algae Symposium -Sept.10 ,2012th 7(Haeckel, 1866, c/o Simonetta
Gribaldo).Olmix Algae Symposium-Sept.10th,20128Olmix Algae
Symposium et al, TREE,Lane -Sept.10th,2012 2008 9 19 21.
Endosymbiotic gene transfer: organelle genomes forge eukaryotic
chromosomes Timmis et Algae Symposium -Sept.10 ,2012 Genetics 5,
123-135 Olmix al. (2004) Nature Reviews th10 between 2 and 1.5
billion years Endosymbiotic gene transfer: organelle genomes forge
eukaryotic chromosomes Timmis et Algae Symposium -Sept.10 ,2012
Genetics 5, 123-135 Olmix al. (2004) Nature Reviews th11 ?about 1,2
billion years20 The life of Algae Symposium -Sept.10 ,2012Olmix
diatoms in the worlds oceans E. Virginia Armbrust Nature 459,
185-192 th12 22. Olmix Algae Symposium -Sept.10th,201213Olmix Algae
Symposium -Sept.10th,201214() (1949)Dr Kathleen Drew-BakerOlmix
Algae Symposium -Sept.10th,201215 21 23. (/) Olmix Algae Symposium
-Sept.10th,201216 Olmix Algae Symposium -Sept.10th,201217 Sauvageau
191722 Olmix Algae Symposium -Sept.10th,201218 24. Source:
IfremerOlmix Algae Symposium
-Sept.10th,201219www.seaweedenergysolutions.com/-Saccharinalatissima
farmingOlmix Algae Symposium -Sept.10th,201220Olmix Algae Symposium
-Sept.10th,201221 23 25. Co-extraction of laminarin, mannitol ,
fucans with alginates AcidCalciumNa2CO3 LAMINARIN MANNITOLFUCANS
Na- ALGINATEFind an alternative to the storage of fresh biomass in
formaldehyde Olmix Algae Symposium -Sept.10th,201222 : 1811
Courtois Production of soda bricks by seaweedharvesters by
burningdried kelps in stoneovens Olmix Algae Symposium
-Sept.10th,201223 Kpper,. et al. (2008) Proc. Natl. Acad. Sci. USA
105, 6954-824 Olmix Algae Symposium -Sept.10th,201224 26. The
Ectocarpus genome (200 Mb) project J.-M. Cock : 3,000,000 reads
(10X, shotgun)cDNA : 100,000 reads (full-length cDNAs) J. M. Cock,
Roscoff P. Rouz / Y. van der Peer - - + Es Genome Consortium - - -
- - AUTOMATIC ANNOTATION- EXPERT ANNOTATIONCock JM et al. (74
authors)Nature. 2010 Jun 3; 465:617-21and more than 20 papers
duringthe two last years.Olmix Algae Symposium -Sept.10th,2012
(HGT)Olmix Algae Symposium -Sept.10th,2012Michel et al. New Phytol.
2010 PMID: 20618907 26 ()Olmix Algae Symposium -Sept.10th,201227 25
27. 78 III (3) 89 E. siliculosus PKS2 E. siliculosus PKS3E.
siliculosus PKS1 81 F. vesiculosus PKS 8876F. spiralis PKSS.
binderiPKS 97 100 (7)99(2) 81 100(9) Type III Polyketide
synthasesChalcone synthases 95Stilbene synthases(28) Resveratrol
synthases Pyrone synthases Acridone synthase 100 Bisphenyl
synthases (HGT)98(10) -() Olmix Algae Symposium -Sept.10th,2012 28
III (PKS) Abundance Extractedionm/z 342 PhloroglucinolBoiledcontrol
1hour 3hours 5hoursTime Olmix Algae Symposium -Sept.10th,2012CNRS
patent sept.2011 n 11 58728 Meslet et al submitted soon. 29 (L.)
C.Agardh , , & JGI 26 Olmix Algae Symposium -Sept.10th,2012 30
28. Red algal genomicsChondrus Porphyra genome genome Jonas
CollnCatherine BoyenOlmix Algae Symposium -Sept.10th,201231Chondrus
crispus flowering (NS, Canada, ASP)Hana-Tsunomata .Hana-Tsunomata
Hana-nori (),,Kaede-nori()andHana-sakura-sou(").Olmix Algae
Symposium -Sept.10th,201232John Bothwell150 MbpFSUJenaOlmix Algae
Symposium -Sept.10th,201233 27 29. Olmix Algae Symposium
-Sept.10th,201234Olmix Algae Symposium
-Sept.10th,201235IDEALGIDEALG 2011-2020 10 . 18 partners: UEB
coordination, SBR:UMR7139-6teams,UMR7144-3teams+FR2424,
CEVAPleubian,AMURE-UBO,IFREMERBrest,UBS-LBCMLorient,Agro-Campus,ENSCR,IRISA-Symbiose(Rennes),CNRSU-Nantes,28
INRA-LBE,1largecompany:Danisco&4SMEs,C-Weed,Aleor,FranceHaliotis,BezhinRosko
Olmix Algae Symposium -Sept.10th,2012 36 30. : , , , , , Olmix
Algae Symposium -Sept.10 ,2012 th37! C. LeblancM. CzjzekJ.M. CockG.
Michel J. CollnC. BoyenB. Kloareg3829 31. : Dr. Mirjam Czjzek1: /
1cmOlmix Algae Symposium-Sept.10th,2012Baldauf (2003) Science2: /O
HO HO O - SO 3 O O OO O - S H O 3 O O - S 3 HOMe O O OSO 3-H HH O
OO- 3OSO OOMeO OH OOOSO 3- OH OO H OHH OOO O O HO OH HO2CO OO
OH3COHO OH O X+-O3SOHnO - SO3 OHO O OOH OOHNHBaldauf (2003)
Science30Olmix Algae SymposiumOOH -Sept.10th,2012 O 3 32. . 4 653
12 4121 , GTGH GT GH Olmix Algae Symposium -Sept.10th,2012.4 :
()()(& ) ()() () /()()()() Olmix Algae Symposium
-Sept.10th,20125 1 % b(1-4)a(1-3) OHO OHOOOO OH OH OSO3- OHO O Ok-O
OOH OH OSO3- OH OOO OO i-OH OSO3- OH OHOSO3-OO Ol-OOSO3- HOOSO3-
Olmix Algae Symposium -Sept.10th,20126 31 33. k- (- )NaI [()][]
Olmix Algae Symposium -Sept.10th,20127 k-() b- k- Olmix Algae
Symposium -Sept.10th,20128 k-() Trp95 Arg151 k-32 Olmix Algae
Symposium -Sept.10th,20129 34. k-Anion exchange chromatographyDP4
DP6 DP8%hydrolysis 0 S364749 5110 60 15 20 2530 Time(min)!0,3
DP4/CitrateDP4%00,218 S4042 0,15662 0 1015 20 25 30 010 20 30
40(min)Olmix Algae Symposium -Sept.10th,2012 (%) 10 k- DP4
DP4DP4Olmix Algae Symposium -Sept.10th,201211 / 2 % Olmix Algae
Symposium -Sept.10th,201212 33 35. Agarose sol/gel transition
()b(1-4) O a(1-3)OH CH2OH O OOO OHO OH agarose n Handbook of
hydrocolloidsOlmix Algae Symposium -Sept.10th,2012(Medin, A. S.
1995)13Zobellia galactanivorans , ()Barbeyron et al. 2001:
(CAZ)CeBiTech 114 (GH), 12 (PL),17 (CE) 72 !16 GH16 Genome Project
MPI Bremen F. O. Glckner, M. Bauer, R. Amann14Olmix Algae Symposium
-Sept.10th,2012 a-CBM6 TPS3 CBM6 TPS3 TPS3 TPS3 TPS3
CBM6GH96Pseudomonas
agaralyticusFlamentetal.(2007)ApplEnvironMicrobiol b- GH-16
X-70UNKAgaAGH-16 AgaBAgaBZobellia
galactanivoransJametal.(2005)BiochemJ k-GH-16Big2 GH-16 CBM16P.
carrageenovora,Z. galactanivorans Barbeyronetal.(1998)MolBiolEvol
i-GH-82GH-82Alteromonas fortis,Z.
galactanivoransBarbeyronetal.(2000)JBiolChem
l-WDrepeatdomainNewGHPseudoalteromonas
carrageenovoraGuibetetal(2007)BiochemJ GH107CadCadCad
UNKMariniflexile fucanivoransColinetal.(2006)Glycobiology
NewPLPseudomonas alginovoraChavagnatetal.(1996)BiochemJ34Olmix
Algae Symposium -Sept.10th,201215 36. Phylogenetic analysis of
Zobellia enzymes fromfamily GH16Zg3628AgaF GH16UNK Zg3640AgaGGH16
Zg1017AgaHGH16 GH16Zg1017 & Zg2600Zg3376AgaI Zg2600AgaE GH16UNK
Zg4267AgaCGH16 Zg4203AgaAGH16X70UNKZg3573AgaB GH16 b-
Zg4243AgaDGH16CBM6 Zg236CgkA GH16CBM16 k- Zg2431LamA
PKDGH16Zg367LamBGH16 Zg1010LamC GH16CBM6Zg1014LamD GH16UNK CBM42 ?
Zg1021 GH16CBM6 UNKZ3347 GH16 LicABlOlmix Algae Symposium
-Sept.10th,201216 , b(1-4)a(1-3)OHOCH2OH O OM. Lahaye and C. Rochas
1991 O OOHO OH O HO HOOH CH2OMeOOHOHO OO O O O O OOHO O OOH OHO OH
b(1-4) b(1-4) a(1-3)CH3 a(1-3) HO2CO OSO3- O O O CH2OMeCH2O O OOO
OO OOHOO OHOH OH,, , , .... Olmix Algae Symposium
-Sept.10th,201217Activity screening on algal cell wall
extractsL6S=L-galactose-6-sulfate LA=3,6-anhydro-L-galactose
L-galactose-6-sulfate() Porphyra spOlmix Algae Symposium
-Sept.10th,201218 35 37. -2 R59W56 G H53 -3 W131E144 L6S -2G-4
-1L6SR133 Olmix Algae Symposium -Sept.10th,2012E139S 19Z.
Galactanivorans () () Olmix Algae Symposium -Sept.10th,2012 20!36
21 38. Nathalie Bourgougnon, Kevin Hardouin, Loannes Le Bars,Gilles
Bedoux, Christel Marty, Justine Dumay*, JP Berg ** Laboratoire de
Biotechnologie et Chimie Marines, UBS, PRES UEB, IUEM * Mer
Molcules Sant, Universit Nantes ** IFREMER, Nantes 1Laboratoire de
Biotechnologie et Chimie Marines 15 /- 10 2 : - / : (, , ,
...)Olmix Algae Symposium -Sept.10th,2012 2Laboratoire de
Biotechnologie et Chimie Marines
Ingnieriedesproduitscosmtiquesetdesant Master
BiotechnologieBiomolcules,micro-organismesandbio-procdsOlmix Algae
Symposium -Sept.10th,2012 3 37 39. ?? , ? ? ? , , Olmix Algae
Symposium-Sept.10th,2012 4 Harvest of marine macroalgae in Brittany
(2009)Brown RedGreenOthers 3.9%1.1%0.3%94.7% 70 000/ CSAVM 2010
-Sangiardi 2010 Olmix Algae Symposium-Sept.10th,2012 5 Technologies
38 Olmix Algae Symposium-Sept.10th,2012 6 40. Olmix Algae Symposium
-Sept.10th,2012 RMN, Malditof. 7 (, )Olmix Algae Symposium
-Sept.10th,2012 8 Olmix Algae Symposium -Sept.10th,2012 9 39 41.
Numbering EC Olmix Algae Symposium -Sept.10th,201210 pH stat pH H2O
pH Olmix Algae Symposium -Sept.10th,201211 () (1v)(1v)() 1.0%
60C3hours40 Olmix Algae Symposium -Sept.10th,201212 42. Olmix Algae
Symposium -Sept.10th,2012 13
AlcalaseEC3.4.21.62Leu-Trp-Lys-Arg-Glu-Ile-Tyr-Phe-Arg-Gln-Ser-Val-Asp-Thr-Ala-Pro-AsnOlmix
Algae Symposium -Sept.10th,2012 14 AlcalaseEC3.4.21.62Protamex
EC3.4.21.62&EC3.4.24.28Leu-Trp-Lys-Arg-Glu-Ile-Tyr-Phe-Arg-Gln-Ser-Val-Asp-Thr-Ala-Pro-AsnOlmix
Algae Symposium -Sept.10th,2012 1541 43. Alcalase EC3.4.21.62
FlavourzymeEC3.4.11.1 ProtamexEC3.4.21.62& EC3.4.24.28
Leu-Trp-Lys-Arg-Glu-Ile-Tyr-Phe-Arg-Gln-Ser-Val-Asp-Thr-Ala-Pro-Asn
Enzymes tested only or in combination Olmix Algae Symposium
-Sept.10th,2012 16 QualityQuantity Non Food Food gradeSeasonality
Geography Grade Olmix Algae Symposium -Sept.10th,2012 17 Define the
objectives of upgrading SEAWEEDS Analysis Quality 42 Olmix Algae
Symposium -Sept.10th,2012 18 44. Define the objectives of upgrading
Olmix Algae Symposium -Sept.10th,2012 19 Estimate the availability
of the biomass Olmix Algae Symposium -Sept.10th,2012General
Strategy20 QualityQuantity Non FoodFood gradeSeasonality Geography
10-24% 38-60%14-29% GradeOlmix100 g Symposium g / Algae MS
-Sept.10th,2012 2143 45. Kjeldhal Lowry and Bradford Gaz FID LeuPhe
Asp AlaLys Tyr ValGlu GlyIle Ser Met ISThr Pro 0 1 23 4 5 6 Temps
(min) Olmix Algae Symposium -Sept.10th,201222 Dubois HPAEC Olmix
Algae Symposium -Sept.10th,201223 23% 15% OILY 0% 24% 5% 2% 20% 44
Olmix Algae Symposium -Sept.10th,201224 46. Quantity 10-24%
38-60%14-29% Seasonality Geography Olmix Algae Symposium
-Sept.10th,2012 25 10-24% 38-60%14-29% Olmix Algae Symposium
-Sept.10th,2012 26 Amadite 11 2 2 , ,, , , ()Olmix Algae Symposium
-Sept.10th,2012 2745 47. () - , , () J. Fleurence & J. Dumay
(MMS), E. Deslandes &V. Stiger (LEMAR), N. Bourgougnon (LBCM),
R. Baron & JP Berg (Ifremer) 28 Olmix Algae Symposium
-Sept.10th,2012 () - Palmaria palmata R- : 4 % 0.4 % Olmix Algae
Symposium -Sept.10th,201229 Collaboration: ! Young researchers:
Kevin Hardouin & Loannes Le Bars Technical assistant: Christel
Marty4630 48. (MSP) -UptadeDr Henri SALMON - Director of research -
INRA 37380 Nouzilly Dr Herv DEMAIS - Scientific advisor - OLMIX
56580 Brhan 1Polysaccharides: Introduction =( ) , [1]. Olmix Algae
Symposium -Sept.10th,20122Polysaccharides= macroamolecules Olmix
Algae Symposium -Sept.10th,2012347 49. (MSP) Green ,,, 3,6,,
Kappa,Iota,Lambda Olmix Algae Symposium -Sept.10th,20124 = () 64 1
32 56 42 1 5 14 1 33 2 1,4> -1,4-> -1,6-> Olmix Algae
Symposium -Sept.10th,20125 (MSP) : -> -> A () ( )
[4)--d-Glcp-(14)--l-Rham3S-(1]n () .
[4)--l-Idop-(14)--l-Rham3S-(1]n 48 Olmix Algae Symposium
-Sept.10th,20126 50. (MSP) :-> -> 12,
4-O-sulphatedfucopyranose)Olmix Algae Symposium -Sept.10th,2012
7MSP Olmix Algae Symposium -Sept.10th,2012 8 I : HSV-1(37) CgCDgD
(VIandVII)-> Olmix Algae Symposium -Sept.10th,2012 9 49 51.
p-KG03MeehyeinKima,
JoungHanYim,So-YeonKima,HaeSooKim,WooGhilLeea,SungJinKim,Pil-SungKangb,Chong-KyoLeeOlmix
Algae Symposium -Sept.10th,201210MSP :MSP [41]. , : /[40] . [42,43]
E[45,46].Olmix Algae Symposium -Sept.10th,201211MSP L- P , ., [44].
. [42,43]. (A) (B)(10mg/kg)3 50Olmix Algae Symposium
-Sept.10th,201212 52. MSP (low MW)C1q C4 [47] D, B, F :Area of
interaction with C4 X -> ->Olmix Algae Symposium
-Sept.10th,201213MSP :PRR (PRRs) Toll
-carrageenanstimulatemouseTcellculturesinatoll-likereceptor-4(TLR4)dependentmannergeneratingaT
helper1(Th1)patternedcytokineresponse.
However,splenocytespreparedfromTLR4-deficientmicestillretainsomeabilitytoproduceinterferon-
in response [40]. to
-carrageenansuggestingthatPRRsotherthanTLR4arealsoelicited.[52]Olmix
Algae Symposium -Sept.10th,201214MSP :iNOS TNF+IFN
Fucoidan(g/ml)TNF+IFNFucoidan(g/ml)Glialcells+TNF+IFN=>iNOS(induciblenitricoxidesynthase)InbitioniNoswithdosesofFucoidan
(56)[53,56,57]2T(Th2).Olmix Algae Symposium -Sept.10th,201215 51
53. MSP : /[53,54,55,56]. +DCChemokinesMCP-1MSP ROS= Botanical
polysaccharides: Macrophage immunomodulation and therapeutic
potential Igor A. Schepetkin, Mark T. Quinn *Olmix Algae Symposium
-Sept.10th,2012 16MSP, 57. Kim, M.H.; Joo, H.G. Immunostimulatory
effects of fucoidan on bone marrow-derived dendritic cells. Immunol
Lett 2008, 115, 138-143. 58. Choi, E.M.; Kim, A.J.; Kim, Y.O.;
Hwang, J.K. Immunomodulating activity of arabinogalactan and
focoidan in vitro. J Med Food 2005, 8, 446-453.Olmix Algae
Symposium -Sept.10th,2012 17MSP:MSP52Olmix Algae Symposium
-Sept.10th,2012 18 54. MSP : MSP (, ) and > [8,9,10 ,11,12].
[13,14,15,16,17]. MSP [16].::40sOlmix Algae Symposium
-Sept.10th,2012 19MPS / [23] [24,25, 26, 22]. [24,26]. [24] [27 ]
.Olmix Algae Symposium -Sept.10th,2012 20MSP () (28,29,30). 21,
LDL-. (29). well-characterizedLPLHL(31). : (32, 33).M.S.P
Santamarina-Fojo S et al. Arterioscler Thromb Vasc Biol
2004;24:1750-1754Olmix Algae Symposium -Sept.10th,2012 2153 55. 1
Olmix Algae Symposium -Sept.10th,2012 22 2 MSP ? MSP(64) : [65].
Olmix Algae Symposium -Sept.10th,2012 23 3 ==54 Olmix Algae
Symposium -Sept.10th,2012 24 56. 25 1. Ooi, V.E.C; Liu, F.
Immunomodulation and Anti-Cancer Activity of Polysaccharide-Protein
Complexes. Current Medicinal Chemistry, 2000, 7, 715-729. 2.
Sharon, N., Lis, H. Scientific American, 1993, pp. 74-81. 3.
Hodgson, J. Biotechnology, 1991, 9, 609-613. 4. Lahaye, M.; Ray, B.
Cell-wall polysaccharides from the marine green alga Ulva rigida
(Ulvales, Chlorophyta)-NMR analysis of ulvanoligosaccharides.
Carbohydr Res 1996, 283,161-173. 5. Percival, E.; McDowell, R.H.
Chemistry and Enzymology of Marine Algal Polysaccharides; Academic
Press: New York, NY, USA, 1967; p. 219. 6. Lahaye, M.; Robic, A.
Structure and functional properties of ulvan, a polysaccharide from
green seaweeds. Biomacromolecules 2007, 8, 1765-1774. 7. Lahaye,
M.; Brunel, M.; Bonnin, E. Fine chemical structure analysis of
oligosaccharides produced by an ulvan-lyase degradation of the
water-soluble cell-wall polysaccharides from Ulva sp. (Ulvales,
Chlorophyta). Carbohydr Res 1997, 304, 325-333. 8. Bernardi, G.;
Springer, G.F. Properties of highly purified fucan. J Biol Chem
1962, 237, 75-80. 9. Springer, G.F.; Wurzel, H.A.; McNeal, G.M.;
Ansell, N.J.; Doughty, M.F. Isolation of anticoagulant fractions
from crude fucoidin. Proc Soc Exp Biol Med 1957, 94, 404-409. 10.
Kusaykin, M.; Bakunina, I.; Sova, V.; Ermakova, S.; Kuznetsova, T.;
Besednova, N.; Zaporozhets, T.; Zvyagintseva, T. Structure,
biological activity,and enzymatic transformation of fucoidans from
the brown seaweeds. Biotechnol J 2008, 3, 904-915. 11. Li, B.; Lu,
F.; Wei, X.; Zhao, R. Fucoidan: structure and bioactivity.
Molecules 2008, 13,1671-1695. 12. Pomin, V.H.; Mourao, P.A.S.
Structure, biology, evolution, and medical importance of sulfated
fucans and galactans. Glycobiology 2008, 18, 1016-1027. 13.
Matsubara, K.; Matsuura, Y.; Bacic, A.; Liao, M.L.; Hori, K.;
Miyazawa, K. Anticoagulant properties of a sulfated galactan
preparation from a marinegreen alga, Codium cylindricum. Int J Biol
Macromol 2001, 28, 395-399. 14. Farias, E.H.C.; Pomin, V.H.;
Valente, A.P.; Nader, H.B.; Rocha, H.A.O.; Mourao, P.A.S. A
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M.E.; DIncecco, A.; Piccoli, A.; Totani, L.; Tinari, N.;
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from brown seaweed.Mol Immunol 1994, 31, 247-253. 48. Clement,
M.J.; Tissot, B.; Chevolot, L.; Adjadj, E.; Du, Y.; Curmi, P.A.;
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showingthe importance of oligosaccharide branching in its
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Algae Symposium-Sept.10th,2012 29 49. Tissot, B.; Montdargent, B.;
Chevolot, L.; Varenne, A.; Descroix, S.; Gareil, P.; Daniel, R.
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Gonnet, F.; Iborra, A.; Berthou, C.; Thielens, N.; Arlaud, G.J.;
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by lambda- carrageenan: toll-like receptor 4/MyD88-dependent and
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249-258. 53. Leiro, J.M.; Castro, R.; Arranz, J.A.; Lamas, J.
Immunomodulating activities of acidic sulphated polysaccharides
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2007, 7, 879-888. 54. Nakamura, T.; Suzuki, H.; Wada, Y.; Kodama,
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Suppression of iNOS expression by fucoidan is mediated by
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Daniel, R. NMR characterization and molecular modeling of fucoidan
showing the importance of oligosaccharide branching in its
anticomplementary activity. Glycobiology 2010, 20, 883-894. 61.
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Isolation, purification, and characterization of fucose-containing
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Selective cleavage and anticoagulant activity of a sulfated fucan:
Stereospecific removal of a 2-sulfate ester from the polysaccharide
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Algae Symposium -Sept.10th,2012 3157 59. Pr. Philippe Bougnoux -
INSERM U 1069, Centre HenryS. Kaplan, University Hospital, Tours,
France [email protected] 1 Olmix Algae Symposium
-Sept.10th,2012 2 Overview DHA DHA DHA DHA DHA
Remodelingoftumorneovasculararchitecture DHA I58 Olmix Algae
Symposium -Sept.10th,2012 3 60. DHA : 3 :Olmix Algae Symposium
-Sept.10th,20124 Olmix Algae Symposium -Sept.10th,20125Likelihood
of response 56 3to chemotherapy *20 15 5FU Odds Ratio*
Fattyacidprofile 543210 DHA median > DHA (%) *; p=0.03 Bougnoux
et al, Br J Cancer, 1999Olmix Algae Symposium -Sept.10th,2012659
61. DHA DHA DHA Olmix Algae Symposium -Sept.10th,20127DHA MDA-MB231
(%) 0 20406080 100 MDA- MB231ControlDocosahexaenoicacid(DHA)
Prooxidants Culturemedium enriched(5d) Antioxidants inDHA
orOleicacid oleicacidDoxorubicin, 10-7 M (Doxo)Doxo+DHA*
Doxo+DHA+Oxidants*Doxo+OxidantsDoxo+DHA+AntioxidantsDoxo+oleicacid+OxidantsDHA
Olmix Algae Symposium -Sept.10th,2012Germain et al., Int J Cancer,
1998 8 PUFA enhance cytotoxic% change in cell toxicity after
doxorubicin efficacy of doxorubicin * 10090 Cytotoxicity, %80 *70 *
60 ** *Fattyacidsalone 4050 2 Fattyacids+20 R =0,829330
**oxidants0010203040 Fattyacids+10hydroperoxides,antioxidants
pmol/g proteins -10LA ALN AA EPA GLA DHA while producing n-6 n-3
n-6 n-3 n-6 n-3 lipoperoxidesFatty acids , 10g/ml Germain et al.,
Int J Cancer,1998 60Olmix Algae Symposium -Sept.10th,20129 62.
Olmix Algae Symposium -Sept.10th,2012 10 Study designDHA 7%/ 8%
DHA8%DHASCO) 8 Adipose tissue76 5.45431.821 0.8 0.10 Palm DHA 0.1
DHA 0.3 DHA 0.8 gggDiet DHAOlmix Algae Symposium -Sept.10th,2012 11
40 20DHA+anti-oxidantsPalmoil 0 1 35 Weeks -20DHA DHA+ -40 oxidants
Bougnoux et al, Lipids, 1999 DHAOlmix Algae Symposium
-Sept.10th,2012 1261 63. : Tumor irradiation : DHA
Tumorsurfacevariation,(%) Single dose, 18 Gy10
Daysfollowingirradiation : 0Diet : -10 024 6810 12 14 16 18 DHA -20
-30Palmoil 18Gy -40 -50DHA -60 -70Colas et al, Int J Cancer 2004
DHA E Olmix Algae Symposium -Sept.10th,2012 13 Olmix Algae
Symposium -Sept.10th,2012 14 LVDP (mmHg)Saline 60 Basal
Pressure50transductorECG 40 30 Volume charge 20 10 0+pro-oxidant
contrle +anti-oxidant 200mmH LVDP (mmHg)25mm/secg 60After
volume10050loading2040 200mmHg30 1002020100 +pro-oxidant contrle
+anti-oxidant omega-3 Diet with 15% fish oil Germain et al.,
Pharmacol. Res, 200362 Olmix Algae Symposium -Sept.10th,2012 15 64.
DHA 1. DHA 2. DHA Maheo et al., Free Rad Biol & Med, 2004 3.
GPx 4. GPx1Vibet et al., Free Rad Biol & Med, 2008 DHA Olmix
Algae Symposium-Sept.10th,201216 DHA?Olmix Algae
Symposium-Sept.10th,201217DHA DHAMammarytumorsinrats TumorSurface
Chemotherapy with docetaxel 20variati on% 10 Breast cancer cell
line MDA-MB-231 MDA-MB 2310 100 Control - 01 DHA - 02 Cell
viability, % 80Control+VitEControl DHA+VitE- 03 60 - 04 40 - 05 20
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nMweeks - - - - 4321 01 23 4 567DHA E DocetaxelU Olmix
ToursSymposium -Sept.10data921 Algae Unpublished ,2012th S. Vibet,
PhD Theses Tours1863 65. DHA (Vibetetal,2008)
(Germainetal,1998,Hardmanetal,2001) (Colas&Bougnoux,2004)
Vincristine(Ikushimaetal,1991),Vinorelbin(Menendezetal2004),
NF-KappaB ,Taxanes(Shaikh&Wahle,2008) :
C:MitomycinC(Pardinietal,1993) CYPs,
Cyclophosphamide(Shao&Pardini,1995) : - (Calderetal,2009) -
Olmix Algae Symposium -Sept.10th,2012 19 DHADHA E(Colas et al.,
Clin Cancer Res 2006)Denis et al., Clin Cancer Res 2003 Goupille et
al, BreastCancer Res & Treat 2012 DHA Olmix Algae Symposium
-Sept.10th,2012 20 :64 Olmix Algae Symposium -Sept.10th,2012 21 66.
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-Sept.10th,201217Olmix Algae Symposium -Sept.10th,201218 109 111. -
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Algae Symposium -Sept.10th,2012 39 118. 4 *+** +***++ 2/PRPSOL
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