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A Perfusion-based Micro 3-D Cell Culture Platform. NTHU MSCL Adviser: C. H. Liu C. He Yeh. A Perfusion-based Micro 3-D Cell Culture Platform. Outline Culture environment at large scale Culture environment at micro scale Principle Fabrication Conclusion & Features - PowerPoint PPT Presentation
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A Perfusion-based Micro 3-D Cell Culture PlatformNTHU MSCLAdviser: C. H. LiuC. He Yeh
A Perfusion-based Micro 3-D Cell Culture PlatformOutlineCulture environment at large scaleCulture environment at micro scalePrincipleFabricationConclusion & FeaturesFurther work
General Culture Equipment (1) (2) (3) (4) (5) (6) (7) (8) (9) (10) (11) (12) (13) (14) (15) Lab On a Chip
On Chip AdvantagesSmall dimensionSaving sampleRapid assay process (micro dimension, avoid medium or reagent diffusion time gradient or concentration different )Cheap, disposable
On Chip Culture RequirementTemperature : 37pH : 7.2 ~ 7.4 ( 95% air 5%CO2 or HEPES)Buffered saline (PBS, PBSA, etc)Medium : (Serum, glucose, amino acids, vitamin, antibiotics )
3D culture (In vivo Culture)
A Perfusion-based Micro 3-D Cell Culture Platform
Working Principle :
A Perfusion-based Micro 3-D Cell Culture PlatformFabrication :
ConclusionFeature 1: 3D well-defined culture environmentmimic an in vivo environmentdistinct cellular behaviors (cell-cell and cell-extracellular interactions )provide more meaningful data
ConclusionFeature 2 : Short Loading cell channel
Feature 3 : High throughput (8 chambers)
Further workBioassay (inject reagent instead of culture medium in vivo bioassay)Biochemical experimentTissue engineeringDrug screening
TAS
ReferenceA PERFUSION-BASEDMICRO 3-D CELL CULTURE PLATFORM S. B. Huang1, M. H. Wu2, Z. F. Cui3, Z. Cui2, J. L. Lin4 and G. B. Lee1*
MICROFLUIDIC DEVICES FOR THE ASSEMBLY AND CULTURE OF THREE-DIMENSIONAL MULTI- CELLULAR CONSTRUCTS WITH DIFFUSION-LIMITED MICROENVIRONMENTS M. Pinelis1, R. W. Kasinskas2, R. T. Borno1, J. H. Park1, E. Chu3, N. S. Forbes2 and M. M. Maharbiz1
"Differentiation of human Embryonic Stem Cells into Hepatocytes in 2D and 3D culture system in vitro" The International Journal of Developmental Biology, Vol. 50, pp. 645-652, 2006
"The multilayered postconfluent cell culture as a model for drug screening" Critical Reviews in Oncology/Hematol, Vol. 36, pp. 147-157, 2000. J. M. Pardon; C.L. van der Wilt; K. Smid; E. Smitskamp-Wilms; H.H. Baukus; P. E. Piazo; G.
"Development of PDMS Microbioreactor with Well-defined and Homogenous Culture Environment for Chondrocyte 3-D Culture M. H. Wu, J. P. G. Urban, Z. Cui and Z. F. Cui, Biomedical Microdevices, Vol. 8, pp. 331-340, 2006.
PS.: (glucose),,,