A T-cell Specific TCR Delta DNA Binding Protein is a Member of the Human GATA Family

28/4/2015 ATcellspecificTCRdeltaDNAbindingproteinisamemberofthehumanGATAfamily.

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC452855/ 1/4

EMBOJ.1991Jul10(7):18091816. PMCID:PMC452855

ATcellspecificTCRdeltaDNAbindingproteinisamemberofthehumanGATAfamily.VJoulin,DBories,JFElouet,MCLabastie,SChrtien,MGMatti,andPHRomo

INSERMU.91,HpitalHenriMondor,Crteil,France.

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Abstract

ThehumanGATA1,hGATA1(previouslycalledNFE1,GF1orEryf1),amajorsequencespecificDNAbindingproteinoftheerythrocyticlineage,isamemberofazincfingerfamilyofDNAbindingproteins.WereportherethecloningofahumancDNAforanewmemberofthisfamily.Thismember,calledhGATA3,has85%aminoacidhomologywithhGATA1intheDNAbindingdomainandnohomologyelsewhereintheprotein.UnlikehGATA1,hGATA3isnotlocalizedontheXchromosomeandwemapittothe10p15bandofthehumangenome.NorthernblotanalysisindicatesthatthisfactorisaTcellspecifictranscriptionfactor,presentbeforeactivationandupregulatedduringTcellactivation.TheencodedhGATA3protein,madeinaninvitrotranscriptiontranslationassay,bindstheWGATARmotifpresentinthehumanTcellreceptor(TCR)deltageneenhancerand,bytransfectioninHeLacells,weshowthathGATA3cantransactivatethisTCRdeltageneenhancer.InterestinglythisenhancerbindsandisalsotransactivatedbyhGATA1.Conversely,thepromoterofthehumanglycophorinB(GPB),whichiserythroidspecificandcontainstwoWGATARmotifs,bindsandistransactivatedbyhGATA1and,toalesserextent,byhGATA3.TheseresultsindicatethattheactivationofspecificgenesbyhGATA1orhGATA3ispartlygovernedbythelineageexpressionofthesetwofactorsduringhaematopoiesisandthat,intheTcelllineage,hGATA3bindsthehumanTCRdeltageneenhancerandisinvolvedinitsexpression.

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A T-cell Specific TCR Delta DNA Binding Protein is a Member of the Human GATA Family