MonsterScript1stStrandcDNASynthesisKitMonsterScriptReverseTranscriptase
1. No RNase H activity
2. Over 15kb mRNA, full-length cDNA
3. , 65C 4. , pg RNA
5. RNase Inhibitor, RNA
1.Cheng, G., et al. (2011) Selec on of Clinicallyrelevant
Protease Inhibitor Resistant Viruses Using the Genotype 2a HCV
Infec on System. An microb. Agents Chemother. , AAC.0138210.
2.Cheng, G., et al. (2011) Selec on of Clinically Relevant Protease
InhibitorResistant Viruses Using the Genotype 2a Hepa s C Virus
Infec on System. An microb. Agents Chemother. 55, 21972205. 3.Chen,
W., et al. (2010) Disrup on of ptLPD1 or ptLPD2, Genes That Encode
Isoforms of the Plas dial Lipoamide Dehydrogenase, Confers Arsenate
Hypersensi vity in Arabidop sis. Plant Physiology 153, 13851397.
4.HernandezDiaz, I., et al. (2010) The Mineralocor coid Receptor Is
a Cons tu ve Nuclear Factor in Cardiomyocytes due to Hyperac ve
Nuclear Localiza on Signals. Endocrinol ogy 151, 38883899.
Kit:
1. MS040910, 10 rxn
2. MS041050, 50 rxn
RTase:
1. MSTA5110, 10 rxn
2. MSTA5124, 24 rxn
MMLVReverseTranscriptase1stStrandcDNASynthesisKitMMLVHPReverseTranscriptase
1.
2. miRNA, 15kb mRNA
3.
4. , 100pg ~ 1ug RNA
5. RNase Inhibitor, RNA
FailSafePCRSystem(PCR !!!)
PCR ?
1. ? 4. ?
2. GC ? 5.?
3. , over 10~20Kb? 6.template ?
FailSafe Selection Kit, 12 (A~L) buffer, FailSafe enzyme
,Epicentre PCR
1.FailSafe PCR PreMix Selection Kit (all 12 buffer)
Cat # FS99060, one enzyme with 12 buffer, selection 4
(total 48 rxns)
2. FailSafe PCR System with PreMix Choice (any one buffer)
Cat # FS99100, one enzyme with 1 buffer, 100 rxns
3. FailSafe Enzyme Only,
Cat # FSE51100, 100 U, 100~200 rxns
Cat # FSE5101K, 1,000 U, 1,000 ~ 2,000 rxns
4. Individual FailSafe PCR buffer
Cat # FSP995(A~L), 2.5ml, 100 rxns
www.biogenesis.com.tw [email protected]
(02)2655-8877 (04)22602466 (037)687493
(07)3422370 (03)8463953 0800-211-667
BDM2011023
1. 75 ()
2. 88
1,000 7-11
Kit:
1. MM070110, 10 rxn
2. MM070150, 50 rxn
RTase:
1. RT80110, 10,000 U, 200 U/ul
2. RT80125K, 25,000 U, 200 U/ul
2011/10/20 2012/2/29
Enzyme Substrate Ac vity Applica ons Concentra on Cat#
DNaseI,(RNaseFree)
dsDNA and ssDNA
In presence of Mg2+, it cleaves each DNA strand randomly and
independently, preferen ally adjacent to pyrimidines. In presence
of Mn2+, it cleaves both strands simultaneously, genera ng
fragments with blunt ends of 12base overhangs.
Removing DNA from RNA preps. 1,000 MBU 5,000 MBU 10,000 MBU
D9902K D9905K D9910K
BaselineZeroDNase
dsDNA and ssDNA
Digests dsDNA or ssDNA down to mononucleo des. Removing DNA from
RNA preps. 1,000 MBU 5,000 MBU
DB0711K DB0715K
EndoculeaseI, ssDNA 3 "5 exonuclease Removal of ssDNA and
oligonucleo des 1,000 U 5,000 U 20,000 U
X40501K X40505K X40520K
EndoculeaseIII dsDNA 35 exonuclease that digests duplex DNA from
the 3 end of a nick or a blunt or 3recessed end; not ac ve on
thionucleo des. Exo III also has 3DNA phosphatase and apurinic DNA
endonuclease ac vi es.
Used with S1 Nuclease or Mung Bean Nuclease to make nested dele
ons. Prepara on of ssDNA templates for sequencing. Sitedirected
mutagenesis. Prepara on of labeled strandspecific probes.
5,000 U 25,000 U
EX4405K EX4425K
EndoculeaseIV dsDNA with an abasic site
Cleaves sugarphosphate bonds 5 of an abasic site DNA repair and
an tumor drug research. Base Excision seq scanning of DNA
containing dUMPs
100 U E70100
EndoculeaseVII ssDNA Exonuclease that digests in both 5"3' and
3"5' direc ons Removal of primers and singlestranded oligos 100 U
250 U
EN510100 EN510250
PlasmidSafeATPDependentDNase
Linear ssDNA and dsDNA
Selec vely digest linear DNA. No ac vity on nicked or
closedcircular DNA
Remove chromosomal DNA fragments from plasmid, fosmid, and
BAC
1,000 U 5,000 U 10,000 U
E3101K E3105K E3110K
RecBCDNuclease,
dsDNA and ssDNA
An ATP and Mg2+dependent exonuclease that digests linear DNA in
both 53 and 35 direc ons. Not ac ve on nicked or closedcircular
dsDNA.
Removal of linear DNA from circular DNA 1,000 U BCD0401K
RecJExonuclease
ssDNA 53 exonuclease that digests ssDNA with a 5 phosphate or a
5OH.
Removal of primers and ssDNA from dsDNA 50 U 250 U
RJ411050 RJ411250
RNaseA ssRNA Cleaves ssRNA 3 of pyrimidine residues. Removal of
RNA from DNA preps. RNase protec on assays. RNA mapping and
structure studies.
5mg/ml, 2ml MRNA092
RNaseI ssRNA Cleaves ssRNA between all dinucleo de pairs.
Removal of RNA from DNA preps. RNase protec on assays. Mismatch
detec on of single basepairs in RNA:RNA or RNA:DNA hybrids.
1,000 U 5,000 U
N6901K N6905K
RNaseIII dsRNA Cleaves dsRNA in presence of Mg2+ to 1215bp
dsRNA. Cleaves dsRNA in presence of 20mM Co2+ or Mn2+ to 1825
Random cleavage of long dsRNA to short dsRNA. RNA interference
(RNAi). Studies on RNA structure,
50 U RN02950
RNaseH RNA in RNA:DNA hybrid
Cleaves RNA in RNA:DNA hybrid without affec ng unhybridized RNA
or DNA
Elimina on of RNA prior to secondstrand cDNA synthesis. Removal
of poly(A) tails from mRNA hybridized to oligo(DT).
250 U 1,000 U
R52250 R0601K
RNaseH,HybridaseThermostable
RNA in RNA:DNA hybrid
Cleaves RNA in RNA:DNA hybrid without affec ng unhybridized RNA
or DNA
RNA mapping and structure studies. Removal of RNA from DNA
preps.
100 U 500 U
H39100 H39500
RNaseR PremRNA The 3 tails of lariats will be trimmed by RNase R
to the branch point nucleo de, where there is a 2,5phosphodiester
linkage. Lariats are produced during premRNA splicing of intron
regions and can be isolated from a mixture of total RNA by diges on
with RNase R
Alterna ve splicing and gene expression studies
Intron cDNA produc on
Intronic screening of cDNA libraries.
250 u RNR07250
Emzyme Liga on Temp
TypeofEndsLigatedConcentra on Cat#
Blunt Cohesive
T4DNALigase 4C25C yes Yes
Cooning 500 U, 10U/ul 1,000 U, 10U/ul 2,000 U, 10U/ul
LH805H LH810H LH820H
FastLinkDNALigase 20C yes Yes Rapid Liga on 25 rxn / 50 rxn /
100 rxn LK11025 / LK0750H / LK6201H
ThermostableDNALigase 20C95C No Yes
Templatedependent liga on 250 U 750 U 25,00U
A32250 A32750 A3202K
CircLigaseIIssDNALigase 20C65C Ligates ssDNA No Make ssDNA
circles 1,000 U / 5,000 U CL9021K / CL9025K
T4RNALigase 37C Ligates ssDNA and ssRNA No Ligate ssRNA to ssRNA
or ssDNA 1,000 U / 2,500 U LR5010 / LR5025
TeermostableRNALigase 25C60C Ligates ssRNA No Ligate ssRNA to
ssRNA or ssDNA 1,000 U TRL8101K
PrimaryApplica on
DNA and RNA Ligases
DNA and RNA ,,
