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behavior of cisplatin Investigated by spectrophotometry and capillary electrophoresis

Hoai Viet NguyenReg..projektu: CZ.1.07/2.3.00/20.0148Nzev projektu: Mezinrodn spoluprce v oblasti "in vivo" zobrazovacch technikNzev:kolitel:Datum:22.02.2013CisplatinPlatinum inorganic complex

Used in the management of non-small lung cancer, malignant melanoma, endometric cancer and adrenocorticol carcinoma.The therapeutic is drastically narrowed.

2Spectrophotometric analysis

Measurements preformed by SPECORD 210 (Analytik Jena, Germany)Absorbtion spectra was form 200 to 700 nmCuvettes : 1cm, Hellma, Essex, UKBGE : - 0.2 M sodium borate (pH 7, 7.5, 8, 8.5,9) - 0.2 M sodium phosphate (pH 6, 6.5, 7, 7.5, 8)Sample : CisPt (500, 400, 300, 200, 100 and 50 M)

3Capillary electrophoresis analysis

Measurements performed by 3D Hwelett Pacard with DAD - Detection at 200, 272, 293 and 360 nmUncoated fused silica capillary was used with ltot= 64.5cm, leff = 56cm with ID = 50 mSeparation condition: voltage 20kV, injection 20 sec by 3.4 kPaBGE: 0.2 M sodium borate (pH 7, 7.5, 8)Sample: CisPt 500, 400, 300, 200, 100 and 50 M

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Fig. 1: Spectra of 500 M CisPt with borate buffer at different pH (7, 7.5, 8, 8.5, 9)Fig. 2: Absorbance of CisPt with different concentrations (500, 400, 300, 200, 100 and 50 M) in borate buffer at different pH (7, 7.5, 8, 8.5 and 9)272nm293nm360nmBorate buffer

5Phosphate buffer

Fig. 3: Spectra of 500M CisPt with phosphate buffer at different pH (6, 6.5 7, 7.5 and 8)Fig. 4: Absorbance of CisPt with different concentrations (500, 400, 300, 200, 100 and 50 M) in borate buffer at different pH (6, 6.5, 7, 7.5 and 8)272nm293nm360nm6Result and discussionFig. 5: Spectra of 500 M CisPt with borate and phosphate buffer at different pH (7, 7.5 and 8)7

e= a - EOFe : Effective electrophoretic mobilitya : Apparent mobility

EOF : Electroosmotic flow mobility

I : Effective capillary length (m)L : Total capillary length (m)t : Migration time (s)V : Electric field (V)

Effective electrophoretic mobility

8Cation +Anion - Result and discussionFig. 6: CE-UV (=200 nm) of Spectra of 500M CisPt with borate buffer at different pH (7, 7.5, 8, 8.5 and 9)

9Effective electrophoretic mobility

Fig 6. Effect of pH on effective electrophoretic mobility. Conditions: 500M CisPt with borate buffer at different pH (8, 8.5 and 9)

1010ConclusionCE-UV detection is powerful method for cisPt analysis.

Absorbance of cisplatin in borate buffer at different pH (9, 8.5, 8, 7.5 and 7) and phosphate buffer at different pH (8, 7.5, 7, 6.5 and 6) was investigated by UV-vis spectrometry.

Behavior of cisplatin in borate buffer at pH 7.5 is different from cisplatin in borate buffer at pH 9, 8.5 and 8. Therefore, there are complicated processes happening at pH 7.5.

11Thanks toIng. Maja StanisavljeviMgr. Marketa Vaculovicova PhDMgr. Lukas Nejdl Prof. Ing.Rene Kizek PhD

Financial support from following projects PGS16_2012, Liga proti rahovine Praha 2012/2013,NANOLABSYSCZ.1.07/2.3.00/20.0148 and CEITEC CZ.1.05/1.1.00/02.0068.

12Thank you for your attention13