BIOLOGICAL INDUSTRIESDO YOU SUSPECT MYCOPLASMA? Prevention, Detection and Treatment of Mycoplasma Contamination

Biological Industries offers you:

MYCOPLASMA DETECTION

EZ-PCR Mycoplasma Test KitCat. # 20-700-10 (10 assays)Cat. # 20-700-20 (20 assays)Ready to use PCR mix for the detection of mycoplasma in cell culturePCR

EZ-PCR Mycoplasma Test Kit is designed to detect the presence of mycoplasma contaminating biological materials, such as cultured cells. EZ-PCRMycoplasma detection by the direct culture procedure is time-consuming and some mycoplasma species are difficult to cultivate. With PCR testing, results are obtained within a few hours, since the presence of contaminant mycoplasma can be easily detected simply by verifying the bands of amplified DNA fragments in electrophoresis. PCRDNA

The primer set allows detection of various mycoplasma species, with high sensitivity and specificity:

TREATMENT OF MYCOPLASMA-INFECTED CELLS WITH ANTIBIOTICS

The contamination of cells with mycoplasma is a very common problem, even though it often goes unnoticed since no cloudiness appears in the cell culture.

Since mycoplasma-infected cells cannot always be discarded, many complicated methods have been suggested for the elimination of the mycoplasma.

Biological Industries is now offering a combination of antibiotics, which have been shown to be effective in the elimination of mycoplasma species that account for 90% of the contamination found in cell cultures. Biological Industries90%BIOMYC-3(cat. # 03-038-1)BIOMYC-1(cat. # 03-036-1)& BIOMYC-2(cat. # 03-037-1)

BIOMYC-1 & BIOMYC-2

BIOMYC-1 is based on the antibiotic tiamutin, which is produced by the fungus pleurotus mutilus. .

BIOMYC-2 is based on minocycline, which is a tetracycline derivative. .

These two antibiotic solutions are generally used sequentially in combination..

BIOMYC-3BIOMYC-3 is based on the ciprofloxacin antibiotic, which is a member of the fluoroquinolone group. Many mycoplasma species have been found to be sensitive to BIOMYC-3.

BIOMYC-3.

PREVENTIONPHARMACIDAL(cat. # IC-110100)Disinfectant solution for incubators and sterile work-benches in cell culture- and molecular biology laboratories AQUAGUARD-1(cat. # 01-867-1)Disinfecting of CO2 incubator water baths AQUAGUARD-2(cat. # 01-916-1E)Disinfecting of ordinary Waterbaths

PHARMACIDALThe problem of contamination in incubators and/or sterile work-benches is often a serious one, leading to extensive damage.Non-toxic and biodegradable, and therefore can be used for spraying incubators while the cells remain insidePrevents contamination with and growth of fungi (and spores), bacteria (and spores) mycoplasma and viruses (including HIV and Hepatitis B) Active ingredients: Quarternary Benzylammonium compounds. The solution does not contain mercury, formaldehyde, phenol or alcohol.HIV&HBV,

AQUAGUARD-1 For disinfecting CO2 incubator water bathsThe water required to create the humidity is a source of contamination which disperses in the incubator. In order to disinfect the water we recommend Aquaguard-1 Solution, which contains a disinfectant that does not cause damage to the stainless steel tray, is non-toxic, non-volatile, and extremely effective.The water should be replaced with sterile water every two to four weeks, adding 50ml of Aquaguard-1 per 5 liters of water.Aquaguard-1550ml Aquaguard-1

AQUAGUARD-2 For prevention of microbial growth in ordinary water baths

Aquaguard-2 is intended for disinfecting various kinds of water baths from bacteria and fungi. Aquaguard-2 It is recommended to use 2ml of Aquaguard-2 for each liter of water in the bath, and to repeat the procedure every 1-2 weeks. After 4-6 weeks, the bath should be emptied and refilled with water containing Aquaguard-2.

2ml/L, 1246Aquaguard-2The active ingredient in Aquaguard-2 is safe to humans and does not cause any irritating effects to the skin when used in the recommended concentration. Aquaguard-2 is biodegradable.Aquaguard-2

MYCOPLASMA FAQs

Why is routine mycoplasma testing important?The cell functions of mycoplasma-infected cells are affected. Since it is difficult to detect infection reliably with other methods, it is possible that results of experiments are being misinterpreted.

Do I have to culture my cells in the absence of antibiotics before testing?

If it is not an urgent case, it is recommended to culture without antibiotics for several days, in order to maximize the strength of the signal, that is observed in PCR.PCR

Can I test cells immediately after they have been removed from liquid nitrogen?It is recommended to let them first grow until 90-100% confluence without antibiotics.90100

How frequently should I test my cells?Whenever new cells enter the labBefore each liquid nitrogen storageRoutinely every 2-3 monthsWeekly after contamination has been found in the laboratoryWhen you believe that cells characteristics have been modified23

Which antibiotic(s) should I use to treat infected cells?Biomyc-1 + Biomyc-2 sequentially, or Biomyc-3 aloneBiomyc-1 + Biomyc-2 Biomyc-3

Biomyc-1, Biomyc-2, Biomyc-3 together cover 90% of the species90

There is no single antibiotic that covers the whole spectrum

We recommend to work in parallel to save time; Biomyc-1 + 2 with half the dishes and Biomyc-3 with the other half. Biomyc-1 + 2 Dish Biomyc-3

How can I be certain that treatment was effective?Grow the cells in media without antibiotics for 2 weeks, and then re-test.