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热门信号通路集锦• Autophagy
• Apoptosis
• Histone modi�cation
• JAK/STAT
• MAPK
• mTOR
• NF-κB
• Oxidative Stress Pathway
• P53
• PI3-Kinase/Akt
• TGF-β
• Toll-like Receptor (TLR)
• Wnt
目 录第一部分:Autophagy Signaling............................................................................................................ 3
第二部分:Apoptosis Signaling............................................................................................................... 7
第三部分:Histone modi�cation.......................................................................................................... 13
第四部分:JAK/STAT Signaling.............................................................................................................. 22
第五部分:MAPK Signaling................................................................................................................... 27
第六部分:mTOR Signaling.................................................................................................................... 30
第七部分:NF-κB Signaling.................................................................................................................. 33
第八部分:Oxidative Stress Pathway.................................................................................................. 38
第九部分:P53 Signaling........................................................................................................................ 40
第十部分:PI3-Kinase/Akt Signaling................................................................................................... 43
第十一部分:TGF-β Signaling...............................................................................................................48
第十二部分:Toll-like Receptor (TLR) Signaling............................................................................... 51
第十三部分:Wnt Signaling...................................................................................................................53
Autophagy Signaling细胞自噬(autophagy) 是近年来生命科学领域的一个研究热点,是真核生物中进化保守的对细胞内物质进
行周转的重要过程。该过程中一些损坏的蛋白或细胞器被双层膜结构的自噬小泡包裹后,送入溶酶体(动物)或液泡(酵母和植物)中进行降解并得以循环利用。
细胞自噬可能由很多因素诱导产生,如饥饿、生长因子缺乏、微生物感染、细胞器损伤、蛋白质折叠错
误或聚集、DNA损伤、放疗、化疗等等。这些信号可以通过mTOR的抑制或AMPK活化来引起细胞自噬。细
胞自噬发生后会形成自噬泡(phagophore),ULK1,2/Atg13复合物促进Atg复合物进入到自噬泡膜上,
ATG14L/Vps34蛋白复合物发生三磷酸化反应,从而募集更多的Atg复合物进入到膜上。接着自噬泡通过泛
素化途径使膜生长延长而形成自噬小体。在自噬小体成熟期间,蛋白酶ATG4降解其脂双层膜上的LC3-II (LC3-PE)。最后自噬体和溶酶体融合,其中的大分子和氨基酸等组分可以重新被细胞利用。
目前普遍认为自噬是一种防御和应激调控机制。细胞可以通过自噬和溶酶体,消除、降解和消化受损、
变性、衰老和失去功能的细胞、细胞器和变性蛋白等。为细胞重建、再生和修复提供必需原料,实现细
胞再循环和再利用。另一方面,自噬在机体的免疫、感染、炎症、肿瘤、心血管疾病、神经退行性疾病
的发病中也具有十分重要的作用。
3
Autophagy Regulators Panel自噬调节剂套装,目录号189488
Leupeptin,Hemisulfate,SyntheticAMPK Inhibitor,Compound CAutophagy Inhibitor,3-MAAutophagy Inducer,STF-62247Ba�lomycin A1,Streptomyces griseusDynamin Inhibitor I,DynasoreDAPK InhibitorHDAC Inhibitor XXIII,Tubastatin AmTOR Inhibitor III,PP242Necrostatin-1Nigericin,Sodium Salt,Streptomyces hygroscopicusNocodazolePi�thrin-μPI-103RapamycinPIKfyve InhibitorSMER28Tamoxifen,4-Hydroxy-,(Z)ThapsigarginCaspase Inhibitor I,Z-VAD-fmkTunicamycin,Streptomyces lysosuper�cusWiskostaitinbpV(phen)DMSO
autophagic proteolytic inhibitorautophagy inhibitorhVps34,autophagosome formation blockerautophagy inducer,target TGN and enhance autolysosomal acidi�cationvacuolar ATPase inhibitor,autophagasome maturation blockervesicle �ssion inhibitor,autophagy blockerautophagy inhibitorHDAC6 selective autophagy inhibitor,autophagosome-lysosome fusion blockermTORC1/2 inhibitor,autophagy inducerNecroptosis blockerionophore,alter intracellular PH,-blocks lysosomal protein degradation
microtubule disruptor,autophagosome-lysosome fusion blockerautophagy inducer,target iHSP70 and inhibit lysosomal functionDNA-PK,PI3-K,and mTORC1/2 inhibitor,autophagasome inducermTOR inhibitor,autophagy inducerslows down the autophagic breakdown of proteinsmTOR-independent autophagy inducerautophagy inducer-estrogen antagonist,increase ceramide & Beclin 1 levelssarco/endoplasmic reticulum Ca2+ATPase inhibitor,ER stress and autophagy inducerapoptotic inhibitorProtein N-glycosylation inhibitor,ER stress and autophagy inducerN-WASP inhibitor,autophagosomes intracelluar trafficking blockerPTEN inhibitor,autophagy suppressor
自噬研究试剂盒Product Catalogue No.
Akt/mTOR/S6K Pathway Explorer Antibody MiniPack
15-104
FlowCellect™ Autophagy LC3 Antibody-based Assay Kit (100 tests)
FCCH100171
FlowCellect™ Autophagy Reagent Pack FCCF200097
FlowCellect™ LC3-GFP Reporter Autophagy Assay Kit (CHO)
FCCH100170
FlowCellect™ LC3-GFP Reporter Autophagy Assay Kit (U2OS)
FCCH100181
Autophagy Detection Reagent Pack CF200097
Autophagy Detection Reagent Pack CF200097
LC3-II Enrichment Kit (Western Blot) 17-10232
LentiBrite™ GFP-LC3-II Enrichment Kit (Flow Cytometry)
17-10230
LentiBrite ™ GFP-LC3 Lentiviral Biosensor 17-10193
LentiBrite ™ GFP-LC3 Control Mutant Lentiviral Biosensor
17-10189
LentiBrite ™ RFP-LC3 Lentiviral Biosensor 17-10143
LentiBrite ™ RFP-LC3 Control Mutant Lentiviral Biosensor
17-10188
mTOR phosphorylation Pathway Explorer Antibody MiniPack
15-105
108976-10mg171260-1mg189490-50mg189497-10mg196000-10μg324410-10mg324788-10mg382187-1mg475988-5mg480065-5mg481990-5mg
487928-10mg506155-10mg528100-1mg553210-1mg524611-5mg573121-10mg579002-5mg586005-1mg627610-1mg654380-10mg681525-1mg203695-10mgKP31817-15ml
组分 靶标 目录号
*以上组分可按目录号单独购买
4
Search for your antibody at: www.merckmillipore.com/antibodies
参考文献:
Increased susceptibility of cytoplasmic over nuclear
polyglutamine aggregates to autophagic
degradation. Iwata, Atsushi, et al. Proc. Natl. Acad.
Sci. U.S.A., 102: 13135-40 (2005) 2005
The p75 neurotrophin receptor can induce autophagy
and death of cerebellar Purkinje neurons.
Florez-McClure, Maria L, et al. J. Neurosci., 24:
4498-509 (2004) 2004
Role of the Apg12 conjugation system in
mammalian autophagy. Mizushima, Noboru, et al.
Int. J. Biochem. Cell Biol., 35: 553-61 (2003) 2003
参考文献:
Increased susceptibility of cytoplasmic over nuclear
polyglutamine aggregates to autophagic
degradation. Iwata, Atsushi, et al. Proc. Natl. Acad.
Sci. U.S.A., 102: 13135-40 (2005) 2005
Role of the Apg12 conjugation system in
mammalian autophagy. Mizushima, Noboru, et al.
Int. J. Biochem. Cell Biol., 35: 553-61 (2003) 2003
Product Catalogue No.
Akt Inhibitor X 124020
AMPK Activator VI, RSVA314 171272
AMPK Inhibitor, Compound C 171260
Autophagy Inducer, STF-62247 189497
Autophagy Inhibitor, 3-MA 189490
Autophagy Regulators Panel 189488-1EA
Ba�lomycin A1, Streptomyces griseus 196000
bpV(phen) 203695
Cathepsin L Inhibitor, CAA0225 219502
DAPK Inhibitor 324788
Dynamin Inhibitor I, Dynasore 324410
ICMT Inhibitor 420350
Leupeptin, Hemisulfate, Synthetic 108976
mTOR Inhibitor III, PP242 475988
Necrostatin-1 480065
Niclosamide 481909
Nigericin, Sodium Salt, Streptomyces hygroscopicus
481990
Nocodazole 487928
PI-103 528100
Pi�thrin-µ 506155
PIKfyve Inhibitor 524611
Rapamycin 553210
SMER28 573121
Spautin-1 567569
Thapsigargin 586005
Wiskostatin 681525
自噬研究抑制剂Product Catalogue No.
Anti-Abl (c-, v-, Bcr-), clone 24-21 MABT203
Anti-APG10 AB15408
Anti-APG5 AB15404
Anti-APG5 AB15404P
Anti-ATG13, clone 2H4.2 MABC46
Anti-ATG16L1 ABC25
Anti-ATG18 (WIPI-2), clone 2A2 MABC91
Anti-ATG3 AB2953
Anti-ATG4A ABC29
Anti-ATG4B ABC32
Anti-ATG4C ABC21
Anti-ATG4D ABC22
Anti-ATG5 ABC14
Anti-ATG5, clone 177.19 MAB2605
Anti-ATG7 AB10511
Anti-ATG9 ABC23
Anti-Beclin-1 (C-term), clone EPR1733Y, rabbit
MABN16
Anti-Beclin-1 AB15417
Anti-Beclin-1, clone 9A1.1 MABC34
Anti-DRAM1 MABC62
Anti-DRAM2 MABC64
Anti-GABARAP AB15415
Anti-GABARAPL1 AB15278
Anti-GATE-16 ABC24
Anti-LC3A (N-term), clone EP1528Y,
rabbit monoclonalMABC177
Anti-LC3A, clone EPR1754, rabbit monoclonal
MABC175
Anti-LC3A/B (N-term), clone EP1983Y, rabbit monoclonal
MABC176
Anti-phospho-ULK1 (Ser555) ABC124
Anti-phospho-ULK1/ATG1 (Ser758) ABC112
自噬研究抗体
Anti-Phospho-p62/SQSTM1(Ser403),Clone 4F6 monoclonal antibody MABC186
Anti-phospho-ULK1(Ser467),clone15D12.3 MABC307
5
LentiBrite™ Lentiviral Biosensors — 实时监测细胞自噬,自噬小体精确定位
用 GFP-LC3 慢病毒颗粒转染 HeLa 细胞。(A)用完全培养基培养细胞;(B)在含有溶酶体抑制剂的 Earle’s Balanced Salt Solution (EBSS) 中饥饿培养。
利用LentiBrite™ GFP- and RFP-LC3,即使是在很难转染的细
胞中,也可以实时监测细胞的自噬情况,并对形成的自噬
小体进行精确定位。
产品优势:
使用方便,重复性好 – 预包被,GFP- & RFP-标签蛋白
复合物
实验稳定 – 长期、稳定的荧光表达
转染高效 - 更高的转染效率,对细胞无干扰,低免疫原性 结果易说明 - LC3突变的慢病毒颗粒,含有转位缺陷蛋
白LC3-G120A,用于对照实验。
•
• • •
货号
17-10193
17-10188
17-10189
17-10143
产品描述
LentiBrite™ GFP-LC3 Lentiviral Biosensor
LentiBrite™ RFP-LC3 Control Mutant
LentiBrite™ GFP-LC3 Control Mutant
LentiBrite™ RFP-LC3 Lentiviral Biosensor
细胞自噬研究创新技术及工具
LC3-II富集前后,用Western blot 对其进行检测 (Catalogue No. 17-10232)
LC3-II Enrichment Kit
— 细胞自噬标志蛋白 LC3-II检测新方法
(WB & Flow Cytometry)
LC3-II 富集试剂盒 (Western Blot)和 LentiBrite™ LC3-II 富集试
剂盒(Flow-Cytometry)使用了选择性渗透的方法,保留了自
噬体上的LC3-II,去除了细胞质中的LC3-I的干扰,从而可
以灵敏准确地对自噬体定量检测 。
货号
17-10230
17-10232
产品描述
LentiBrite™ GFP-LC3-II Enrichment Kit
LC3-II Enrichment Kit (Western Blot)
6
Apoptosis Signaling对凋亡的逃逸能力,是绝大多数癌症的特征。许多蛋白,包括细胞表面受体、配体、蛋白酶和有丝分裂
组分,调节着细胞的生存与死亡的精细平衡。在癌症中,这些感应分子或作用分子的突变,会破坏这个
平衡,从而逃脱凋亡的调控。
细胞凋亡的不同时期具有特征性的生化及形态学上的改变,可以通过多种实验方法和技术检测细胞凋
亡。由于细胞凋亡常常具有复杂的机制,在特定的实验体系中建议使用两种及以上的不同方法进行验
证。默克密理博可以为您提供凋亡研究的全面解决方案及实验技术(如质膜改变,线粒体改变,caspase活化,DNA断裂等)。
7
}
Granzyme
Perf
orin Granzyme A
Granzyme B
Phosphatidylserine Phosphatidylserine
Caspase 9
Procaspase 3 Caspase 3
NME1
SET Complex
p50 p65
p50 p65
IκB
IKK
Akt
Smac/Diablo
HtrA2/Omi tBid
Bid
Caspase 8
Ca2+
p50
FLIPcIAPBcl2
p53
SurvivinBidBak/BaxNoxa/Puma
p65
MDM2
p70S6K
Akt
AktmTOR
Cell Shrinkage
PIP2
PIP3
PDK1
p90RSK
ERK1/2
MEK
Raf
Ras
PI3K
Procaspase 3, 6, 7
IAPp35CrmA
PerforinGranzyme
Caspase 3, 6, 7
Nur
77/T
R3 cJunATF2
JNK/SAPK
CeramideSphingo-myelinase
Elk1
Fos
CREB
elF-4B
AIF W
OX1
Endo
nucl
ease
G
His
tone
H1.
2
NUCLEUS
APOPTOSOME
Growth Factor
TNFα
TNFR
FasLFAS
Fas Decoy
Receptor
BaxBax
PP
P
PP
P
P
P
P
P
PP
PP
PP
P
P
P
PP
PP
Bax Bad
Bcl2Bcl2
ICAD
CAD
CAD
ICAD
CAD
TRAF2RIP
PARP
Bax
TRAD
Procaspase 8
Puma Noxa
Cytotoxic T Cell
1
2
3
4
4
5
78
9
ATP
Apaf-1
Cyt c
∆ Ψm
Late Stage: DNA fragmentation
Nuclear collapse
Formation of apoptotic bodies
Phagocytosis by macrophages
Early Stage:
Loss of mitochondrial membrane potential (∆Ψm)
Mid Stage: Activation of caspases (3,6,7)
Poly-ADP-Ribose Polymerase (PARP) cleavage
Cell shrinkage
Activation of nucleases
Cytochrome c & ATP release
Activation of caspase 8 and 9
12
3
4
56
7
8
910
11
12
Translocation of phosphatidyl-serine to the outer leaf of plasma membrane
抗体
Name Description
Anti-AIF Antibody, internal domain
Anti-Apaf-1 Antibody, CT
Anti-Arginase 1 Antibody
Anti-Bad Antibody, clone BYC001
Anti-BAFF receptor Antibody
Anti-Bak Antibody, NT
Anti-Bax (NT) Antibody
Anti-Bax Antibody
Anti-Bcl2 Antibody, clone 100
Anti-Bcl-X Antibody, clone 2H12
Anti-Beclin Antibody1, clone 4E10.1
Anti-Bid Antibody
Anti-Bim Antibody, internal epitope,
pan-Bim isoforms
Anti-Blys/TALL-1/BAFF/THANK Antibody
Anti-Bmf Antibody
Anti-Calpain Antibody, small subunit of
µ- or m-Calpains (Calpain I or II), clone
P1
Anti-Caspase 1 Antibody
Anti-Caspase 1 Antibody
Anti-Caspase 10 Antibody, clone 16C5.1
Anti-Caspase 12 Antibody, clone 14F7
Anti-Caspase 2 Antibody, clone 10C6
Anti-Caspase 3 Antibody
Anti-Caspase 3 Antibody,
active (cleaved) form
Anti-Caspase 8 Antibody
AB16501
AB16503
ABC897
05-605
ABC337
06-536
ABC11
AB2915
05-729
MAB4625
MABC139
AB1735
AB17003
07-167
ABC348
MAB3083
AB1871
06-503-I
MABC192
MABC555
MAB3501
06-735
AB3623
AB1879
Key Applications
Immunohistochemistry
(Paraf�n);Western Blotting
Western Blotting
Western Blotting;
Immunohistochemistry;
Immunocytochemistry
Immunoprecipitation;Western Blotting
Western Blotting;
Immunohistochemistry
Immunocytochemistry;Immunohistoch
emistry;Immunoprecipitation;Western
Blotting
Western
Blotting;Immunoprecipitation;Immuno
cytochemistry;Immunohistochemistry
Western Blotting;
Immunohistochemistry (Paraf�n)
Flow Cytometry; Western Blotting
Immunohistochemistry;
Flow Cytometry;
Immuno�uorescence;Immunoprecipita
tion;WesternBlotting;
Immunohistochemistry
Western Blotting
Western
Blotting;Immunoprecipitation;
Immunohistochemistry
Western Blotting
Western Blotting
Western
Blotting;Immunohistochemistry
ELISA;Western Blotting;
Immunocytochemistry;
Immunohistochemistry
Immuno�uorescence;Immunoprecipita
tion;Western Blotting
Western Blotting;
Immunohistochemistry (Paraf�n)
Western Blotting;
Immunohistochemistry
Western Blotting;
Immunocytochemistry;
Immunohistochemistry
ELISA;Flow Cytometry;
Immunoprecipitation;Western Blotting
Western Blotting
Immuno�uorescence;Immunohistoche
mistry;Immunohistochemistry
(Paraf�n);Western Blotting
Western Blotting
Species Reactivity
Human;Mouse;Rat
Human;Mouse;Rat
Human;Mouse
Human;Mouse
Human;Mouse;Rat
Human;Rat;Mouse
Human;Bovine;Feline;
Chimpanzee;Canine
Mouse
Human;Canine;Feline
Human;Mouse;Pig;Rat
Human;Mouse;Rat
Human;Mouse;Rat;Rabbit
Human;Mouse;Rat
Human
Human
Bovine;Human
Human;Mouse;Rat
Human
Human;Rat;Mouse
Mouse;Human;Rat
Canine;Human;Mouse;Monkey
Human;Mouse;Rat
Human;Mouse;Rat
Bovine;Human;Mouse;
Monkey;Rat
Qty/Pk
100 µg
100 µg
100 µL
200 µg
100 µg
200 µg
200 µg
100 µL
100 µg
50 µg
100 µg
100 µL
100 µg
100 µg
100 µg
100 µL
50 µg
100 µg
100 µg
100 µg
100 µg
200 µg
50 µg
50 µg
8
抗体
Name Description
Anti-Caspase 9 Antibody, clone 2-23
Anti-Caspase-10 Antibody
Anti-Caspase-14 Antibody
Anti-Caspase-8 (active form p18 subunit)
Antibody, clone 2B12.1
Anti-Cathepsin D Antibody
Anti-COX2 , clone EPR468(2), Rabbit
Monoclonal Antibody
Anti-COX4 Antibody
Anti-c-Rel (NFκB) Antibody
Anti-Cytochrome C Antibody
Anti-Death-associated protein 1
Antibody
Anti-DR4/TRAIL-R1 Antibody
Anti-FADD Antibody, clone 1F7
Anti-FAF1 Antibody
Anti-Fas Antibody (human, activating),
clone CH11
Anti-Fas Ligand Antibody
Anti-Fractin Antibody, CT
Anti-GADD153/DDIT3/CHOP Antibody
Anti-IRE1 Antibody
Anti-Kallikrein 2/KLK2 Antibody, clone
4F2.3
Anti-Kallikrein-1/KLK1 Antibody, clone
11C7.1
Anti-Kallikrein-13/KLK13 Antibody,
clone 33.1
Anti-Kallikrein-3 (KLK3) (CT) Antibody
Anti-Kallikrein-5 Antibody/KLK5
Anti-Mitofusin-2 (Mfn2) Antibody
Anti-p53 Upregulated Modulator of
Apoptosis (PUMA) Antibody
Anti-PARP Cleaved Form Antibody
Anti-PARP Poly ADP-ribose Polymerase-1
Antibody
Anti-Phospho- Bad (Ser75) Antibody
Anti-phospho Beclin Antibody (Thr119)
MAB4709
ABC343
ABC841
MAB10754
06-114
MABC754
AB10526
09-040
AB3547
ABC259
06-744
05-486
ABC346
05-201
AB16982
AB3150
ABC955
ABC241
MABC563
MABC552
MABC971
AB10428
ABC470
ABC42
ABC158
AB3620
AB16661
AB10423
ABC118
Key Applications
Immunoprecipitation;Western
Blotting;Immunohistochemistry
(Paraf�n)
Western Blotting
Western Blotting;
Immunohistochemistry
Western Blotting;
Immunocytochemistry
Western Blotting;
Immunocytochemistry
Western Blotting
Western Blotting;
Immunohistochemistry (Paraf�n)
ELISA;Electrophoretic Mobility Shift
Assay;Western Blotting
Western Blotting;
Immunocytochemistry
Western
Blotting;Immunohistochemistry
Western Blotting
Western Blotting
Western Blotting;
Immunohistochemistry
Flow Cytometry; Western Blotting
Immunocytochemistry;
ELISA;Western Blotting
Western Blotting;
Immunocytochemistry;
Immunohistochemistry (Paraf�n)
Western Blotting
Western Blotting
Western Blotting;
Immunohistochemistry
Western Blotting;
Immunohistochemistry (Paraf�n)
Immunohistochemistry;
ELISA
Western Blotting
Western Blotting;
Immunohistochemistry
Western Blotting
Western Blotting;
Immunohistochemistry
Western Blotting
ELISA;Western Blotting
Western Blotting
Western Blotting;Peptide Inhibition
Assay
Species Reactivity
Human;Mouse
Human
Human
Human
Human
Human
Human
Human
Canine;Human;Rat;Rabbit
Mouse;Human
Human;Mouse;Rat
Human;Mouse
Human;Rat
Human
Human;Mouse;Rat
Human;Mouse;Rat
Human
Human
Human
Human
Human
Human;Mouse;Rat
Human;Mouse
Human;Rat;Mouse
Human
Human
Bovine;Human;Mouse;Rat
Mouse;Rat
Human
Qty/Pk
100 µg
100 µg
100 µg
200 µg
100 µg
100 µL
100 µg
100 µg
100 µg
100 µg
200 µg
100 µg
100 µg
50 µg
50 µg
100 µL
100 µL
100 µg
100 µg
100 μg
100 µg
100 µg
100 µg
100 µg
200 µg
50 µg
100 µL
100 µg
100 µg
11
9
抗体
Name Description
Anti-phospho Mitofusin2 Antibody
(Ser442)
Anti-phospho TRADD (Ser215) Antibody
Anti-Phospho-Bad (Ser134) Antibody
Anti-phospho-Bcl-2 (Ser70) Antibody,
clone 69-10C-2-10C-18, rabbit
monoclonal
Anti-Phospho-Bid (Ser65) Antibody
Anti-phospho-IRE1 (Ser724), clone
EPR5253, Rabbit Monoclonal Antibody
Anti-Poly ADP-ribose Antibody, clone
10H
Anti-RIAP-3 (XIAP) Antibody
Anti-RIP3 Antibody, clone 6E6.2
Anti-Smac/Diablo Antibody, clone 3A9.1
Anti-Survivin Antibody
Anti-Survivin Antibody
Anti-TNFα Receptor Antibody, clone
13F9.1
Anti-TRADD Antibody, clone 1G6.1
Anti-TRAF1 Antibody, clone 1F3
Anti-Traf2 Antibody
Anti-TRAF4 Antibody, clone 5MLN-2H1
Anti-TRAIL Antibody, clone N2B2
Anti-TRAIL/CD253 Antibody, clone 6D12.2
Anti-VDAC Antibody
ABC963
ABC461
AB10422
05-843R
AB2994
MABC742
MABC547
07-753
MABC28
MABC174
AB3610
AB3611
MABC615
MABC988
MABC260
ABC47
MABC985
MABC12
MABC147
AB10527
Key Applications
Western Blotting;Peptide Inhibition
Assay
Western Blotting;Peptide Inhibition
Assay
Western Blotting
Western
Blotting;Immunoprecipitation;
Flow Cytometry
Western Blotting
Western Blotting;Flow Cytometry
Western Blotting;
Immunoprecipitation;Immuno�uoresc
ence
Western Blotting
Western
Blotting;Immunohistochemistry
Western Blotting;
Immunohistochemistry
Western
Blotting;Immunoprecipitation;
Immunohistochemistry
Western Blotting
Western Blotting;
Immunohistochemistry
Western Blotting;
Immunohistochemistry (Paraf�n)
Western Blotting;
Immunohistochemistry;Flow Cytometry
Western Blotting;
Immunocytochemistry
Western Blotting;
Immunohistochemistry;
Immunocytochemistry
Flow Cytometry
Flow Cytometry;
Immunohistochemistry
Western Blotting
Species Reactivity
Human
Human
Human
Human
Human
Human
Human
Human;Rat
Human;Rat
Human
Human
Mouse
Human;Mouse
Human;Rat
Monkey;Human
Human;Mouse;Rhesus
Macaque
Human;Mouse
Mouse
Human
Human;Mouse;Rat;Bovine;
Horse;Rhesus;Macaque;
Chimpanzee;Pig
Qty/Pk
100 µg
100 µL
100 µg
100 µL
100 µg
100 µL
50 µg
200 µg
200 µL
100 µg
100 µL
100 µg
100 μg
100 μg
100 µL
100 µg
100 µL
100 µg
100 µg
100 µg
10
抑制剂
货号 产品描述
Apoptosis Inhibitor II, NS3694
APT1 Inhibitor, palmostatin B
BAX CHANNEL BLOCKER
Bax Channel Blocker II, iMAC2
Bax-Inhibiting Peptide, V5
Bax-Inhibiting Peptide, Negative Control
Bcl-xL BH4(4-23) (Human), Cell-Permeable
Caspase-2 Inhibitor I
Caspase-1 Inhibitor VI
Caspase-1 Inhibitor VI
Caspase-3 Inhibitor III
Caspase-8 Inhibitor II
Caspase-8 Inhibitor II
Caspase-9 Inhibitor I
Caspase-9 Inhibitor I
Caspase-8 Inhibitor I, Cell-Permeable
Caspase-3/7 Inhibitor I
Caspase-3/7 Inhibitor II
InSolution(TM) Caspase-8 Inhibitor II
InSolution(TM) Caspase-9 Inhibitor I
Caspase Inhibitor VI
Caspase Inhibitor VI
InSolution(TM) Caspase Inhibitor VI
Caspase-3 Inhibitor VII
b-Catenin/Tcf Inhibitor III, iCRT3
CFTR Inhibitor III, OXO-172
Caspase-3 Inhibitor I
InSolution Caspase-3 Inhibitor I, Cell-P
Histone Methyltransferase EZH2 Inhibitor, DZNep
Caspase-3 Inhibitor II
178494-10MGCN
178501-5MGCN
196805-5MGCN
196806-10MGCN
196810-5MGCN
196811-5MGCN
197217-1MGCN
218744-1MGCN
218746-1MGCN
218746-250UGCN
218750-1MGCN
218759-1MGCN
218759-250UGCN
218761-1MGCN
218761-250UGCN
218773-1MGCN
218826-1MGCN
218832-1MGCN
218840-250UGCN
218841-250UGCN
219007-1MGCN
219007-250UGCN
219011-1MGCN
219012-10MGCN
219332-10MGCN
219672-10MGCN
235420-1MGCN
235427-1MGCN
252790-2MGCN
264155-1MGCN
货号 产品描述
Caspase-3 Inhibitor II
Fas/FasL Antagonist, Kp7-6
Foxo1 Inhibitor, AS1842856
GSK-3 beta Inhibitor XXIV
Granzyme B Inhibitor I
Histone Acetyl Transferase Inhibitor VII, CTK7A
Heat Shock Factor 1 Inhibitor, KRIBB11
Caspase-1 Inhibitor I
Caspase-1 Inhibitor I
Caspase-1 Inhibitor II
Caspase-1 Inhibitor IV
HIF-Hydroxylase Inhibitor, DMOG
HIF-PHD Inhibitor II, JNJ-42041935
IL-6/Stat3 Signaling Inhibitor, Cpd 6a
IRE1 Inhibitor I, STF-083010
Kir2.1 Inhibitor, ML133
Necrostatin-1
Necrostatin-1, Inactive Control
MLKL Inhibitor, Necrosulfonamide
NOX Inhibitor III, VAS2870
Omi/HtrA2 Protease Inhibitor, Ucf-101
Pi�thrin-a
Pi�thrin-a
Pi�thrin-a, Cyclic-
PICK1 PDZ Domain Inhibitor, FSC231
InSolution Q-VD-OPh, Non-O-methylated
ROS Inhibitor, YCG063
Caspase Inhibitor I
Caspase Inhibitor I
264155-250UGCN
341291-25MGCN
344355-10MGCN
361567-5MGCN
368050-1MGCN
382115-10MGCN
385570-10MGCN
400010-1MGCN
400010-5MGCN
400012-5MGCN
400015-1MGCN
400091-50MGCN
400093-10MGCN
407625-5MGCN
412510-10MGCN
422689-5MGCN
480065-5MGCN
480066-5MGCN
480073-25MGCN
492000-10MGCN
496150-10MGCN
506132-10MGCN
506132-5MGCN
506134-10MGCN
529531-10MGCN
551476-1MGCN
557354-5MGCN
627610-1MGCN
627610-5MGCN
Milliplex kit
Early Apoptosis-7 Plex(Cat.No.48-669MAG)AB2
Analyte Total Phosphorylated
Akt/PKB
BAD
Bcl-2
Active Caspase 8
Active Caspase 9
JNK/SAPK1
p53
(Ser473)
(Ser112)
(Ser70)
(Thr183/Tyr185)
(Ser46)
H,M,R
H
H
H
H
H,M,R
H
Human Late Apoptosis-3 Plex(Cat.No. 48-670MAG)AB1
Analyte Total Phosphorylated
Active Caspase 3
Cleaved PARP
GAPDH
H,M
H
H
11
4500-0450/4500-0455
PF032-1EA
PF036-1EA
4500-0250
APT142/APT242
475866-1KIT
QIA87-1KIT
CBA077-1KIT
FCCH100110
H0XPSMAG-16K
RM0XPSMAG-17K
QIA23-1EA
FCCS025108
48-669MAG
QIA90/QIA92-1KIT
218790-1KIT
APT131/APT165
QIA70-1KITQIA91/QIA93
17-198
17-367
APT423/APT403
4500-0540
QIA108-1KIT
QIA113-1KITAPT428/APT408
4500-0550QIA71-1KIT
APT129/APT171
APT139/APT173
218824-1KIT
QIA72-1KIT
APT172
QIA115-1KITAPT429/APT409
4500-0560
S7100/S7101
S7110/S7111
QIA33-1EA
QIA39-1EA
4500-0121
APT151
AM41-1EA
QIA20-1EA
Guava Nexin Reagent
ANNEXIN V FITC APOPTOSISDETECTION KIT
ANNEXIN V BIOTIN APOPTOSISDETECTION KIT
Guava® MitochodrialDepolorization Kit
MITOLIGHT MITOCHNDRAPPTSIS DET KIT
MITOCAPTURE APOPTOSISDETECTION KIT
CYTOCHROME C RELEASEAPOPTOSIS ASSAY KIT
INNOCYTE CYTOCHROME CRELEASE KIT
FlowCellect Cytochrome C KitMILLIPLEX MAP Human
Oxidative Phosphorylation(OXPHOS) Magnetic Bead Panel
MILLIPLEX MAP Rat/MouseOxidative Phosphorylation
(OXPHOS) Magnetic Bead Panel
BCL-2 ELISA KIT
FlowCellect™ Bcl-2 ActivationDual Detection Kit
MILLIPLEX MAP Human EarlyApoptosis Magnetic Bead
6-Plex Kit
CASPASE DETECTION KITCASPASE-1 ASSAY KIT,
COLORIMETRICCaspase 3 Colorimetric Activity
Assay Kit, DEVDCASPASE-3 ACTIVITY ASSAYCASPASE-3 DETECTION KIT
Caspase 3 Activity Detection KitCaspase 3/7 Assay Kit
(Ac-DEVD-AMC Substrate)CaspaTag Caspase 3,7 In Situ
Assay Kit, FluoresceinGuava® Caspase 3/7 FAM Kit
Caspase-7 Immunoassay/ActivityKit Fluorometric
CASPASE-8 DETECTION KITCASPATAG CASPASE 8 ASSAY
Guava® Caspase 8 FAM KitCASPASE-8 ACTIVITY ASSAY
Caspase 8 Colorimetric ActivityAssay Kit, IETD
Caspase 9 Colorimetric ActivityAssay Kit, LEHD
CASPASE-9 ASSAY KIT,COLORIMETRIC
CASPASE-9 ACTIVITY ASSAYCaspase 9 Fluorometric Activity
Assay Kit, LEHDCASPASE-9 DETECTION KIT
CASPATAG CASPASE 9 ASSAYGuava® Caspase 9 FAM Kit
ApopTag® Peroxidase In SituApoptosis Detection Kit
ApopTag® Fluorescein In SituApoptosis Detection Kit
TDT FRAGEL TM DNA FRAGMENT.DETECT. KIT
FLUORESCEIN FRAGEL DNA FRAG.DETECT. KIT
Guava TUNEL Kit (100 Tests)APOPTOTIC DNA LADDER
DETECTION KITSuicide Track™ DNA Ladder
Isolation KitCell Death Detection (Nuclear
Matrix Protein) ELISA Kit
默克密理博凋亡试剂盒选择指南
早期凋亡
中期凋亡
凋亡时期 货号 品名 检测对象 样本类型 检测方法/所需设备 产品优势 备注
默克密理博为您提供凋亡研究的整体解决方案,包括凋亡检测试剂盒,诱导剂,抑制剂/激活剂,抗体,蛋白和酶等等。产品详情请参考:www.merckmillipore.com
* 红色标记的为现货产品
反应次数不同
晚期凋亡
磷脂酰丝氨酸
线粒体膜电位
细胞色素C
线粒体氧化磷酸化
Bcl-2
Active Caspase 8 (Asp384), Active Caspase 9(Asp315), Akt (Ser473), BAD (Ser112), Bcl-2(Ser70), JNK (Thr183/Tyr185), p53 (Ser46)
总Caspase
Caspase 1
Caspase 3
Caspase 3/7
Caspase 7
Caspase 8
Caspase 9
DNA片段化
NMP
活细胞
活细胞
活细胞
活细胞
活细胞
活细胞
细胞或组织裂解液
活细胞
活细胞
细胞或组织裂解液
细胞或组织裂解液
血清,血浆,细胞培养上
清,细胞或组织裂解液
活细胞
血清,血浆,细胞培养上
清,细胞或组织裂解液
活细胞
细胞或组织裂解液
细胞或组织裂解液
细胞或组织裂解液
活细胞
细胞或组织裂解液
细胞或组织裂解液
活细胞
活细胞
细胞或组织裂解液
活细胞
活细胞活细胞
细胞或组织裂解液
细胞裂解液
细胞裂解液
细胞或组织裂解液
细胞或组织裂解液
细胞裂解液
活细胞
活细胞
活细胞
固定细胞或组织切片
活细胞,固定细胞或组织
切片
固定细胞或组织切片
活细胞,固定细胞或组织
切片
活细胞
贴壁或悬浮细胞
贴壁或悬浮细胞
细胞培养上清
流式细胞仪
流式细胞仪,荧光显微镜
流式细胞仪,荧光显微镜
流式细胞仪
流式细胞仪,荧光显微镜
流式细胞仪,荧光显微镜
免疫印迹
流式细胞仪,荧光显微镜
流式细胞仪
Luminex仪器
Luminex仪器
酶标仪
流式细胞仪
Luminex仪器
流式细胞仪,荧光显微镜
酶标仪
酶标仪
荧光酶标仪
流式细胞仪,荧光显微镜
荧光酶标仪
荧光酶标仪
流式细胞仪,荧光显微镜
流式细胞仪
荧光酶标仪
流式细胞仪,荧光显微镜
流式细胞仪,荧光显微镜
流式细胞仪
荧光酶标仪
酶标仪
酶标仪
酶标仪
荧光酶标仪
荧光酶标仪
流式细胞仪,荧光显微镜
流式细胞仪,荧光显微镜
流式细胞仪
光学显微镜
荧光显微镜,流式细胞仪
光学显微镜
荧光显微镜,流式细胞仪
流式细胞仪
DNA胶
DNA胶
酶标仪
一步法,离心次数少,减少假阳性
有快速结合试剂,离心次数少,减少假阳性
实验简单,易操作
细胞凋亡新的检测方法
细胞凋亡新的检测方法
产品选择多,包括总的caspase检测和caspase家族成员分别检测。实验方法选择多,可以用活细胞进行流式或显微镜检测,也可用酶标仪对细胞或组织裂解液进行检测。
产品选择多,包括总的caspase检测和caspase家族成员分别检测。实验方法选择多,可以用活细胞进行流式或显微镜检测,也可用酶标仪对细胞或组织裂解液进行检测。
高性价比,多选择性
试剂盒组分齐全,有阴阳性对照,灵敏度高
实验次数不同
标记荧光颜色不同
实验次数不同
标记荧光颜色不同
标记荧光颜色不同
实验次数不同
实验次数不同
标记荧光颜色不同
实验次数不同
S7100是核心试剂,S7101是全组分
S7100是核心试剂,S7101是全组分
直接取细胞上清检测,细胞还可以用于其他检测
12
Histone Modi�cations最常见和研究最透彻的组蛋白修饰包括甲基化、乙酰化、磷酸化和泛素化。组蛋白修饰广泛调控着DNA转录、修复、重组和复
制,并可改变染色质结构。
乙酰化
甲基化
磷酸化
泛素化
瓜氨酸化
组蛋白乙酰化酶(HAT)和去乙酰化酶(HDAC)是基因表达与功能调控的重要因素。转录激活复合物所包含的HAT可
将组蛋白含有的赖氨酸乙酰化并打开染色质结构以启动转录。HDAC则可移除乙酰基团,从而抑制基因表达。
特定组蛋白残基的甲基化是常染色质和转录处于激活状态的重要标志,而其他的甲基化事件则标志着异染色质和转
录的抑制。组蛋白甲基化可被位点特异性地组蛋白去甲基化酶去除,如LSD1,UTX,JMJD等酶家族。组蛋白的甲基
化与去甲基化作用从时间和空间上调控着基因表达,尤其是在胚胎发育过程中。
组蛋白磷酸化通常发生在有丝分裂时染色体缩合期和DNA损伤导致的细胞凋亡过程中。然而,特定组蛋白位点的磷
酸化与早期基因感应信号相关,这表明组蛋白磷酸化可能对位点或细胞环境信号产生应答,介导染色质结构的打开
与关闭。
泛素化作用参与某些组氨酸甲基化过程,而泛素化作用需要泛素激活酶(E1)、泛素结合酶(E2)和泛素-蛋白连接
酶(E3)的参与。我们为您提供包括特异性识别泛素连接和组蛋白修饰残基的抗体在内的各种泛素化相关产品。
瓜氨酸是一种经特殊修饰的精氨酸,它可能与类风湿关节炎和多发性硬化等疾病的发生相关。10%的组氨酸带有瓜
氨酸修饰,说明瓜氨酸在基因调控中起作用。默克密理博为您提供针对瓜氨酸修饰的组蛋白H4的特异性抗体及用于
检测这种修饰的抗体和方法。
C
120110
100
90
80
N
1020
30
40
50
N
C
10
20
30
40
50
60
80
90
100
CP
PMe
MeMe
Me
Ac
Ub
70
80
90
100
110 120
Ub
N
10
2030
40
P
P
P
P
P
P
P
Me
Ac
MeAc
Ac
Ac
Me
MeAc
Ac
50
Me Me
Me
Me
H2B(P62807*)
H2A(P0C0S8*)
H3(P68431*)
H4(P62805*)
N
140
CP
PP
P
120130
H2A.X(P16104*)
C
130
120
110
100
90
Me2
P
P
P
P
P
P
MeMeMe
MeMe
Me
Ac
Ac
Ac
Ac
Ac
Ac
Ac
Ac
Me
MeAc
Ac
Ac
AcMe
Me
AcAc
Ac
Ac
AcAc
Ac
Ac
Me
Me
AcAc
Ac
Ac
Ub
P
P
P
P MeMe
Ac
AcAc AcAc
Ac
Me
Me
10
20
30
40
80
Me2
Me2
Me2
Me3
AcAc
Me2
Me3
Me3
Me2 Me2
Me2
Me2
Me2
Me3
Me2
Me3
Me2Me2
MeAc
Me2
Me3
MeAc
Me2
Me3
Acetylation
Methylation
Dimethylation
Trimethylation
PhosphorylationP
Me
Me2
Me3
Ac Ub
*
Ubiquitination
Amino acids buried in nucleosome core
Solvent-accessible amino acids
UniprotKB Accession Number
Download an interactive version of this poster at: www.millipore.com/histonemodapp
13
DescriptionName Key Applications Species Reactivity Qty/Pk
未修饰组蛋白抗体
MAB3864
05-457
05-629
07-146
06-131907-627AB1002209-862
07-59407-371
AB162305-1352
07-680
06-755
05-1341
05-499
07-690
04-928
05-928
09-838
ABE154
07-108
07-59604-858
05-858R
05-858
MAB3422
MABE71
MAB1276
Anti-DNA/Histone H1 Antibody
Anti-Histone H1 Antibody, clone AE-4Anti-Histone H1o/H5 Antibody, clone 3H9Anti-Histone H2A (acidic patch) AntibodyAnti-Histone H2A.Bbd AntibodyAnti-Histone H2A.X AntibodyAnti-Histone H2A.X AntibodyAnti-Histone H2A.Z Antibody
Anti-Histone H2A.Z AntibodyAnti-Histone H2B Antibody
Anti-Histone H2B AntibodyAnti-Histone H2B Antibody, clone 5HH2-2A8Anti-Histone H2B Antibody, testis variantAnti-Histone H3 Antibody
Anti-Histone H3 Antibody, (Unmodified Lys4), clone CMA301
Anti-Histone H3 Antibody, clone 6.6.2Anti-Histone H3 Antibody, CT, panAnti-Histone H3 Antibody, CT, pan, clone A3S, rabbit monoclonalAnti-Histone H3 Antibody, CT, pan, clone A3S, rabbit monoclonalAnti-Histone H3.3 Antibody
Anti-Histone H3.1/H3.2 Antibody
Anti-Histone H4 Antibody
Anti-Histone H4 (citrulline 3) Antibody Anti-Histone H4 Antibody, pan, rabbit monoclonalAnti-Histone H4 Antibody, pan, clone 62-10C-2, rabbit monoclonalAnti-Histone H4 Antibody, pan, clone 62-141-13, rabbit monoclonalAnti-Histone Antibody, clone H11-4Anti-Histone Antibody, Pan, clone F152.C25.WJJAnti-Nuclei & Chromosomes Antibody, histone H1 protein, clone 1415-1
ELISA;Flow Cytometry;Immunocytochemistry;ImmunohistochemistryELISA;Immunocytochemistry;Western Blotting
Western Blotting;Immunocytochemistry
Western Blotting;Chromatin Immunoprecipitation (ChIP)Western BlottingPeptide Inhibition Assay;Western BlottingWestern Blotting;Flow CytometryWestern Blotting
Western BlottingWestern Blotting;Chromatin Immunoprecipitation (ChIP)Western Blotting;ImmunohistochemistryWestern Blotting;Immunocytochemistry
Western Blotting
Western Blotting;Immunocytochemistry;Immunoprecipitation;Chromatin Immunoprecipitation (ChIP)Western Blotting;Immunocytochemistry;ELISA;Immunoprecipitation;Flow Cytometry;Dot BlotWestern Blotting
Western Blotting;Chromatin Immunoprecipitation (ChIP)Western Blotting;Chromatin Immunoprecipitation (ChIP)Western Blotting;Chromatin Immunoprecipitation (ChIP)Western Blotting;Immunocytochemistry;Dot Blot;Chromatin Immunoprecipitation (ChIP)Western Blotting;ChIP-seq;Chromatin Immunoprecipitation (ChIP)Western Blotting
Western Blotting;ImmunohistochemistryWestern Blotting;Multiplexing;Chromatin Immunoprecipitation (ChIP)Western Blotting
Western Blotting;Multiplexing;ChIP-seq; ChIP
Immunocytochemistry;Immunohistochemistry;Western BlottingWestern Blotting;ELISA
ELISA;Western Blotting;Immunocytochemistry;Immunohistochemistry (Paraffin)
All
Human;Bovine;Green PlantsBovine;Human;Mouse;Rat
Mouse;Rat;Human;Chicken;Yeast (S.cerevisiae)Human;MouseHumanHuman;Mouse;Rat;BovineChicken;Human;Zebrafish;Rabbit;Rat;Xenopus;MouseHumanChicken;Xenopus;Human;Yeast (S. cerevisiae)HumanMouse;Human;Rat
Human;Mouse
Human;Rat;Mouse
Human;Vertebrates
Vertebrates;Green Plants;HumanChicken;Human;Mouse;Rat;Yeast (S. cerevisiae)Chicken;Human;Mouse;Rat;Yeast (S. cerevisiae)Human;Chicken;Rat;Mouse;Yeast (S. cerevisiae)Human;Mouse
Mouse;Human
Mouse;Human;Bovine;Avian;Chicken;XenopusHuman;VertebratesChicken;Human;Vertebrates;Yeast (S. cerevisiae)Human
Human;Chicken;Vertebrates;Yeast (S. cerevisiae)Mammals;Human;Bovine;Rat;Xenopus;Opossum;HamsterHuman;Bovine
Human;Rat
100 µg
200 µg
100 µg
200 µL
100 µg200 µL100 µL100 µg
100 µL200 µg
100 µL100 µL
100 µL
100 µg
100 µg
200 µg
100 µL
100 µL
100 µL
100 µg
100 µg
200 µL
200 µL100 µL
100 µL
100 µL
100 µg
100 µg
100 µL
14
DescriptionName Key Applications Species Reactivity Qty/Pk
乙酰化组蛋白抗体
05-1334
ABE18
05-1232
05-1355
07-376
07-290
07-289
AB10020
07-373
07-564
07-382
06-599
07-539
07-353
06-911
07-354
07-355
07-360
07-540
07-750
07-352
06-942
07-593
16-658
06-866
06-598
07-595
04-119
07-329
07-327
04-118
Anti-Acetyl Histone H3 (Lys27) Antibody, clone CMA309Anti-acetyl Histone H3 (Lys9) AntibodyAnti-acetyl Histone H4 (Lys16) Antibody, clone 4E10.2Anti-acetyl Histone H4 (Lys5/8/12/16) Antibody, clone 3HH4-4C10Anti-acetyl-Histone H2A AntibodyAnti-acetyl-Histone H2A (Lys5) AntibodyAnti-acetyl-Histone H2A (Lys9) AntibodyAnti-acetyl-Histone H2A.X (Lys5) AntibodyAnti-acetyl-Histone H2B AntibodyAnti-acetyl-Histone H2B (Lys120) AntibodyAnti-acetyl-Histone H2B (Lys5) AntibodyAnti-acetyl-Histone H3 Antibody
Anti-acetyl-Histone H3 (Lys 4) AntibodyAnti-acetyl-Histone H3 (Lys14) AntibodyAnti-acetyl-Histone H3 (Lys14) AntibodyAnti-acetyl-Histone H3 (Lys18) AntibodyAnti-acetyl-Histone H3 (Lys23) AntibodyAnti-acetyl-Histone H3 (Lys27) AntibodyAnti-acetyl-Histone H3 (Lys36) AntibodyAnti-acetyl-Histone H3 (Lys79) AntibodyAnti-acetyl-Histone H3 (Lys9) AntibodyAnti-acetyl-Histone H3 (Lys9) AntibodyAnti-acetyl-Histone H3 (Lys9/18) AntibodyAnti-acetyl-Histone H3 Antibody, Agarose ConjugateAnti-acetyl-Histone H4 Antibody
Anti-acetyl-Histone H4 Antibody
Anti-acetyl-Histone H4 (Lys12) AntibodyAnti-acetyl-Histone H4 (Lys12) Antibody, rabbit monoclonalAnti-acetyl-Histone H4 (Lys16) AntibodyAnti-acetyl-Histone H4 (Lys5) AntibodyAnti-acetyl-Histone H4 (Lys5) Antibody, rabbit monoclonal
Human;Vertebrates
Human;Mouse;Chicken
Human
Human;Mouse;Rat
Human
Human
Human;Vertebrates
Human
Human
Human
Human
Human;Mouse;Rat
Human
Human;Yeast (S. cerevisiae)Human;Eukaryote
Human;Vertebrates;Yeast (S. cerevisiae)Human;Vertebrates;Yeast (S. cerevisiae)Human;Vertebrates;Yeast (S. cerevisiae)Human
Human
Human;Vertebrates;Yeast (S. cerevisiae)Human;Mouse;Rat
Bovine;Human;Mouse;Yeast (S. cerevisiae)Human;Mouse;Tetrahymena
Human;Tetrahymena;EukaryoteTetrahymena;Human
Human;Yeast (S. cerevisiae)Human;Vertebrates
Human;Mouse;Rat
Human
Human;Vertebrates
100 µg
100 µg
100 µg
100 µL
100 µL
100 µL
200 µL
100 µg
100 µg
100 µL
100 µg
100 µg
100 µL
100 µL
200 µg
100 µL
100 µL
100 µL
100 µL
100 µL
100 µL
200 µg
200 µL
200 µL
200 µL
200 µg
100 µL
100 µL
100 µL
100 µL
100 µL
ELISA;Immunocytochemistry;Immunoprecipitation;Western BlottingWestern Blotting;Dot Blot;Multiplexing; ChIP;ChIP-seqWestern Blotting;Peptide Inhibition Assay
Western Blotting;Immunocytochemistry
Western Blotting
Western Blotting
Western Blotting
Western Blotting;Multiplexing
Western Blotting
Dot Blot;Western Blotting;Peptide Inhibition AssayWestern Blotting;Dot Blot;ImmunoprecipitationWestern Blotting;Immunocytochemistry;ChIP-seq; ChIPMultiplexing;Western Blotting;Peptide Inhibition Assay;ChIP-seq;Dot BlotWestern Blotting;ChIP-seq;Dot Blot;Multiplexing; ChIPImmunocytochemistry;Immunoprecipitation;Western BlottingWestern Blotting;Dot Blot; ChIP;ChIP-seq
Western Blotting;Dot Blot; ChIP
Western Blotting;ChIP-seq; ChIP;Dot Blot
Western Blotting;Multiplexing
Western Blotting;ELISA
Western Blotting;Multiplexing;ChIP-seq; ChIP
Western Blotting; ChIP;Dot Blot;Flow Cytometry;ChIP-seqWestern Blotting;Multiplexing; ChIP
Immunoprecipitation
Western Blotting;Immunocytochemistry; ChIP
Immunocytochemistry;Immunoprecipitation;Western Blotting; ChIPDot Blot;Western Blotting;ChIP-seq
Western Blotting;Dot Blot; ChIP
Western Blotting;Multiplexing;Peptide Inhibition Assay;Dot Blot;ChIP-seq; ChIPWestern Blotting;Dot Blot;Immunocytochemistry;ChIP-seq; ChIPWestern Blotting
15
超过50%的文章使用该抗体进行实验
参考文献:
Baf60c drives glycolytic metabolism in the muscle
and improves systemic glucose homeostasis
through Deptor-mediated Akt activation.
Meng, Zhuo-Xian, et al. Nat. Med., 19: 640-5 (2013)
2013 GATA2 Is a Critical Transactivator for the
Human IL1RL1/ST2 Promoter in Mast Cells/
Basophils: OPPOSING ROLES FOR GATA2 and
GATA1 IN HUMAN IL1RL1/ST2 GENE EXPRESSION.
Yosuke Baba,Keiko Maeda,Takuya Yashiro,Eisuke
Inage,Kazumi Kasakura,Ryuyo Suzuki,Fran
Niyonsaba,Mutsuko Hara,Atsushi Tanabe,Hideoki
Ogawa,Ko Okumura,Yoshikazu Ohtsuka,Toshiaki
Shimizu,Chiharu Nishiyama,François Niyonsaba
The Journal of biological chemistry 287 2012
超过50%的文章使用该抗体进行实验
参考文献:
Acetylation limits 53BP1 association with damaged
chromatin to promote homologous recombination.
Tang, Jiangbo, et al.
Nat. Struct. Mol. Biol., 20: 317-25 (2013) 2013
Chromatin Modulation at the FLO11 Promoter of
Saccharomyces cerevisiae by HDAC and Swi/Snf
Complexes. Ramón R Barrales,Philipp Korber,Juan
Jimenez,José I Ibeas Genetics 191 2012
DescriptionName Key Applications Species Reactivity Qty/Pk
DescriptionName Key Applications Species Reactivity Qty/Pk
甲基化组蛋白抗体
07-328
04-557
Anti-acetyl-Histone H4 (Lys8) AntibodyAnti-acetyl-Histone H4 Antibody, pan (Lys 5,8,12), rabbit monoclonal
Human;Yeast (S. cerevisiae)Human
100 µL
100 µL
Immunoprecipitation;Western Blotting;Dot Blot;ChIP-seqWestern Blotting
04-791
ABE10
07-436
06-1416
ABE101
07-450
07-395
05-1248
07-448
07-548
07-748
07-1570
04-735
07-608
MABE19
ABE250
05-1338
05-1249
07-1584
07-214
04-077
05-808
07-585
Anti-mono/di/trimethyl-Histone H3 (Lys4) Antibody, clone AW304, rabbit monoclonalAnti-monomethyl Histone H3 (Arg8) AntibodyAnti-monomethyl-Histone H3 (Lys4) Antibody
Anti-monomethyl-Histone H3 (Lys9) AntibodyAnti-monomethyl-Histone H3 (Lys9) AntibodyAnti-monomethyl-Histone H3 (Lys9) AntibodyAnti-monomethyl-Histone H3 (Lys9) AntibodyAnti-monomethyl Histone H3 (Lys9) Antibody, clone CMA306Anti-monomethyl-Histone H3 (Lys27) AntibodyAnti-monomethyl-Histone H3 (Lys36) AntibodyAnti-monomethyl-Histone H4 (Lys20) AntibodyAnti-monomethyl Histone H4 (Lys20) AntibodyAnti-monomethyl-Histone H4 (Lys20) Antibody, clone NL314, rabbit monoclonalAnti-dimethyl Histone H3 (Lys23) AntibodyAnti-dimethyl Histone H3 (Lys27) Antibody, clone 8H2Anti-dimethyl Histone H3 (Lys4) AntibodyAnti-Dimethyl Histone H3 (Lys4) Antibody, clone CMA303
Anti-Dimethyl Histone H3 (Lys9) Antibody, clone CMA307
Anti-dimethyl Histone H4 (Lys20) AntibodyAnti-dimethyl-Histone H3 (Arg17) AntibodyAnti-dimethyl-Histone H3 (Arg17) Antibody, rabbit monoclonalAnti-dimethyl-Histone H3 (Arg2) Antibody, rabbit monoclonalAnti-dimethyl-Histone H3 (Arg2) Antibody
Human;Vertebrates
Human
Human;Vertebrates
Human
Human
Human;Mouse;Chicken
Human
Human
Human;Vertebrates
Vertebrates
Human
Human
Human
Human
Human
Human;Mouse;Rat;Chicken
Human;Vertebrates
Human;Vertebrates
Human
Human;Vertebrates
Human;Vertebrates
Human
Chicken;Human
100 µL
200 µL
200 µg
100 µg
100 µg
100 µg
100 µL
100 µg
200 µg
100 µL
200 µg
100 µg
100 µl
100 µL
100 µg
100 µL
100 µg
100 µg
100 µg
100 µL
100 µL
100 µL
200 µL
Western Blotting;Peptide Inhibition Assay;Multiplexing; ChIP
Western Blotting;Peptide Inhibition Assay
Immunocytochemistry;Immunofluorescence;Peptide Inhibition Assay;Western Blotting;ChIP-seq;Dot BlotWestern Blotting;Immunocytochemistry;Peptide Inhibition AssayWestern Blotting;Dot BlotImmunocytochemistry;Peptide Inhibition Assay;Dot Blot;Immunocytochemistry;Peptide Inhibition Assay;Western BlottingWestern Blotting
Western Blotting;Peptide Inhibition Assay
Dot Blot;Immunocytochemistry;Western Blotting;ChIP-seqDot Blot;Immunofluorescence;Western Blotting;MultiplexingWestern Blotting;Multiplexing;ImmunocytochemistryWestern Blotting;Dot Blot; ChIP;Peptide Inhibition AssayWestern Blotting;Multiplexing
Western Blotting;Dot Blot;Multiplexing
Western Blotting;ELISA;Immunocytochemistry
Western Blotting;Immunocytochemistry;Dot Blot; ChIP;ChIP-seqWestern Blotting;Immunoprecipitation;Dot Blot;Immunocytochemistry;ELISA;Multiplexing; ChIPWestern Blotting;ELISA;Immunocytochemistry;Immunoprecipitation;Multiplexing; ChIPWestern Blotting
ELISA;Immunocytochemistry;Western Blotting
Western Blotting
Dot Blot;Western Blotting;Peptide Inhibition Assay;MultiplexingWestern Blotting;Peptide Inhibition Assay
16
DescriptionName Key Applications Species Reactivity Qty/Pk
04-808
07-215
07-452
04-821
07-274
07-030
04-790
05-684
04-835
05-768R07-212
07-441
16-187
04-768
07-947
07-367
05-672
07-528
05-809
ABE44
05-1951
05-1339
07-992
07-952
05-1250
04-809
07-449
Anti-dimethyl-Histone H3 (Arg2), clone 20.2 Antibody, rabbit monoclonalAnti-dimethyl-Histone H3 (Arg26) AntibodyAnti-dimethyl-Histone H3 (Lys27) AntibodyAnti-dimethyl-Histone H3 (Lys27) Antibody, clone 614M, rabbit monoclonalAnti-dimethyl-Histone H3 (Lys36) Antibody
Anti-dimethyl-Histone H3 (Lys4) Antibody
Anti-dimethyl-Histone H3 (Lys4) Antibody, clone AW30, rabbit monoclonalAnti-dimethyl-Histone H3 (Lys4) Antibody, clone RR302Anti-dimethyl-Histone H3 (Lys79) Antibody, clone NL59, rabbit monoclonalAnti-dimethyl-Histone H3 (Lys9) Antibody, rabbit monoclonal
Anti-dimethyl-Histone H3 (Lys9) AntibodyAnti-dimethyl-Histone H3 (Lys9) Antibody, biotin conjugateAnti-dimethyl-Histone H3 (Lys9) Antibody, clone MC554, rabbit monoclonalAnti-dimethyl-Histone H4 (Arg3) Symmetric AntibodyAnti-dimethyl-Histone H4 (Lys20) AntibodyAnti-dimethyl-Histone H4 (Lys20) Antibody, clone 6G7/H4Anti-trimethyl (Lys79)-phospho (Thr80)-Histone H3 AntibodyAnti-trimethyl (Lys9)-phospho (Ser10)-Histone H3 Antibody, rabbit monoclonalAnti-trimethyl Histone H3 (Lys27) Antibody
Anti-trimethyl Histone H3 (Lys27) Antibody, clone 18E9.1Anti-Trimethyl Histone H3 (Lys4) Antibody, clone CMA304Anti-trimethyl Histone H3 (Lys4/Lys9) AntibodyAnti-trimethyl Histone H3 (Lys79) AntibodyAnti-Trimethyl Histone H3(Lys9) Antibody, clone CMA308Anti-trimethyl(Lys9)-phospho(Ser10)-Histone H3 Antibody, rabbit monoclonalAnti-trimethyl-Histone H3 (Lys27) Antibody
Dot Blot;Multiplexing;Peptide Inhibition Assay;Western Blotting
Western Blotting;Multiplexing
Multiplexing;Cell Function Assay;Western Blotting;ChIP-seq;Dot BlotWestern Blotting;Peptide Inhibition Assay
ChIP;Western Blotting;Dot Blot
Dot Blot;Immunocytochemistry;Immunofluorescence;Western Blotting;Peptide Inhibition Assay; ChIPWestern Blotting;ChIP-seq; ChIP;Dot Blot;Peptide Inhibition Assay
Western Blotting
Western Blotting;Dot Blot;Multiplexing;ChIP-seq; ChIP
Western Blotting;Peptide Inhibition AssayImmunocytochemistry;Western Blotting
Dot Blot;Immunocytochemistry;Peptide Inhibition Assay;Western BlottingWestern Blotting;Immunocytochemistry
Immunofluorescence;Western Blotting;Peptide Inhibition Assay;Dot Blot
Western Blotting;Immunocytochemistry;Dot BlotImmunofluorescence;Western Blotting;ImmunocytochemistryImmunoprecipitation; Western Blotting;ImmunohistochemistryWestern Blotting;Peptide Inhibition Assay;Multiplexing;Dot BlotWestern Blotting;Peptide Inhibition Assay;Multiplexing
Western Blotting;Immunocytochemistry;Peptide Inhibition Assay;Dot BlotWestern Blotting;Immunocytochemistry;Dot BlotELISA;Immunocytochemistry;Immunoprecipitation;Western Blotting;Multiplexing;Dot BlotWestern Blotting;Immunocytochemistry
Western Blotting;Dot Blot;Multiplexing
Western Blotting;ELISA;MultiplexingImmunocytochemistry;Immunoprecipitation;Western Blotting;Peptide Inhibition Assay;Dot Blot;Multiplexing
Immunocytochemistry;Immunoprecipitation;Multiplexing;Western Blotting;ChIP-seq; Immunohistochemistry
Human;Vertebrates
Human;Tetrahymena;Vertebrates;XenopusHuman
Vertebrates
Mouse;Human;Chicken;Drosophila;Vertebrates;Yeast (S. cerevisiae)Human;Tetrahymena
Human;Chicken
Chicken;Human;Vertebrates
Human;Vertebrates
HumanHuman;Chicken;Vertebrates;Yeast (S. cerevisiae)Human;Mouse;Rat;Chicken;Yeast (S. pombe)Human;Yeast (S. cerevisiae);ChickenHuman
Human;Mouse;Rat
Vertebrates
Bovine;Human;Mouse;Rat
Human
Human
Human;Mouse
Human;Mouse
Human;Vertebrates
Human;Mouse;Rat
Human
Human;Vertebrates
Human
Human;Mouse
200 µL
100 µL
200 µg
100 µL
200 µL
200 µL
100 µL
400 µL
100 µL
100 µL100 µL
100 µg
100 µL
100 µL
100 µg
200 µL
200 µg
100 µL
100 µL
100 µg
100 µL
100 µg
100 µg
100 µg
100 µg
100 µL
200 µg
17
90%的文章使用该抗体进行实验。
参考文献:
PRC2 is recurrently inactivated through EED or
SUZ12 loss in malignant peripheral nerve sheath
tumors.
Lee, W; Teckie, S; Wiesner, T; Ran, L; Prieto Granada,
CN; Lin, M; Zhu, S; Cao, Z; Liang, Y; Sboner, A; Tap,
WD; Fletcher, JA; Huberman, KH; Qin, LX; Viale, A;
Singer, S; Zheng, D; Berger, MF; Chen, Y; Antonescu,
CR; Chi, P Nature genetics 46 1227-32 2014 XACT,
a long noncoding transcript coating the active X
chromosome in human pluripotent cells. Vallot,
Céline, et al. Nat. Genet., 45: 239-41 (2013) 2013
超过90%的文章使用该抗体进行实验
参考文献:
Early-stage epigenetic modi�cation during somatic
cell reprogramming by Parp1 and Tet2.
Claudia A Doege,Keiichi Inoue,Toru Yamashita,
David B Rhee,Skylar Travis,Ryousuke Fujita,Paolo
Guarnieri,Govind Bhagat,William B Vanti,Alan
Shih,Ross L Levine,Sara Nik,Emily I Chen,Asa
Abeliovich Nature 488 2012 The human GFI136N
variant induces epigenetic changes at the Hoxa9
locus and accelerates K-RAS driven myeloproliferative
disorder in mice.
Cyrus Khandanpour, Joseph Krongold, Judith Sch,
Frederique Bouwman, Lothar Vassen, Marie-Claude
Gaudreau, Riyan Chen, Fernando J Calero-Nieto,
Evangelia Diamanti, Rebecca Hannah, Sara E Meyer,
H Leighton Grimes, Bert A van der Reijden, Joop H
Jansen, Chandrashekhar V Patel, Justine K Peeters,
Bob L, Ulrich D, Bertie G, Tarik M
Blood 120 2012
DescriptionName Key Applications Species Reactivity Qty/Pk
07-549
05-801
07-473
05-745R
04-745
07-442
07-523
05-1242
07-463
04-079
05-1354
Anti-trimethyl-Histone H3 (Lys36) AntibodyAnti-trimethyl-Histone H3 (Lys36) Antibody, clone MC86, rabbit monoclonalAnti-trimethyl-Histone H3 (Lys4) AntibodyAnti-trimethyl-Histone H3 (Lys4) Antibody, clone 15-10C-E4, rabbit monoclonal
Anti-trimethyl-Histone H3 (Lys4) Antibody, clone MC315, rabbit monoclonalAnti-trimethyl-Histone H3 (Lys9) Antibody
Anti-trimethyl-Histone H3 (Lys9) AntibodyAnti-trimethyl-Histone H3 (Lys9) Antibody, clone 6F12-H4
Anti-trimethyl-Histone H4 (Lys20) AntibodyAnti-trimethyl-Histone H4 (Lys20), Antibody, rabbit monoclonalAnti-Histone H3 Antibody (Dimethyl K9, Phospho S10), clone 6HH3-2C5
Dot Blot;Western Blotting
Dot Blot;Western Blotting;Peptide Inhibition Assay
Dot Blot;Western Blotting;ChIP-seq;Chromatin Immunoprecipitation (ChIP)Western Blotting;Chromatin Immunoprecipitation (ChIP);ChIP-seq;Dot Blot;ELISA;Peptide Inhibition Assay;Multiplexing Western Blotting;Dot Blot;Multiplexing;ChIP-seq
Dot Blot; Immunocytochemistry;Multiplexing;Peptide Inhibition Assay;Western Blotting;ChIP-seqDot Blot;Peptide Inhibition Assay;Western BlottingPeptide Inhibition Assay;Immunofluorescence;Dot Blot;ChIP-seq;Western Blotting; ChIPImmunoprecipitation;Western Blotting;Dot Blot; ChIPWestern Blotting;Dot Blot; ChIP
Western Blotting;Immunocytochemistry
Human
Chicken
Human;Vertebrates
Human
Human
Human;Mouse;Rat;Chicken
Human
Human;Mouse
Human;Mouse;Rat
Human;Vertebrates
Human;Drosophila
100 µL
100 µL
100 µL
100 µL
100 µL
100 µg
200 µL
200 µL
100 µg
100 µL
100 µL
DescriptionName Key Applications Species Reactivity Qty/Pk
磷酸化及泛素化组蛋白抗体
05-1315
07-081
06-597
05-1324
05-636
16-193
16-202A
07-1590
07-191
16-189
05-806
Anti-phospho (Ser10) acetyl (Lys14) Histone H3 Antibody, clone 10H10.2Anti-phospho (Ser10)-acetyl (Lys14)-Histone H3 AntibodyAnti-phospho-Histone H1 AntibodyAnti-phospho-Histone H1 Antibody, clone 12D11Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, biotin conjugateAnti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, FITC conjugateAnti-phospho Histone H2A.X Antibody (Tyr142)Anti-phospho-Histone H2B (Ser14) AntibodyAnti-phospho-Histone H3 (Ser10) Antibody, biotin conjugateAnti-phospho-Histone H3 (Ser10) Antibody, clone 3H10
Western Blotting
Immunocytochemistry;Western Blotting;ChIP-seq; ChIPImmunocytochemistry;Western Blotting
Immunohistochemistry;Immunocytochemistry;Immunoprecipitation;Western BlottingImmunocytochemistry;Immunofluorescence;Western Blotting; ChIPImmunocytochemistry;Western Blotting
Flow Cytometry;Immunocytochemistry
Western Blotting;Dot Blot
ELISA;Immunocytochemistry;Immunohistochemistry;Western BlottingWestern Blotting;Immunocytochemistry
Flow Cytometry; Western Blotting;Immunocytochemistry;Immunofluorescence;Peptide Inhibition Assay;Multiplexing
Human
Mouse;Human;Eukaryote
Bovine;Human
Human;Bovine;Mouse;VertebratesVertebrates
Human
Human
Human;Mouse
Human
Human
Human
100 µg
100 µL
200 µg
200 µL
200 µg
100 µg
100 µg
100 µg
200 µL
100 µg
100 µg
18
近70%的文章使用该抗体进行实验
参考文献:
Acetylation limits 53BP1 association with damaged
chromatin to promote homologous recombination.
Tang, Jiangbo, et al.
Nat. Struct. Mol. Biol., 20: 317-25 (2013)
ROS-induced DNA damage and PARP-1 are
required for optimal induction of starvation-
induced autophagy.
José Manuel Rodríguez-Vargas,María José
Ruiz-Magaña,Carmen Ruiz-Ruiz,Jara Majuelos-
Melguizo,Andreína Peralta-Leal,María Isabel
Rodríguez,José Antonio Muñoz-Gámez,Mariano
Ruiz de Almodóvar,Eva Siles,Abelardo López
Rivas,Marja Jäättela,F Javier Oliver Cell research 22
2012
DescriptionName Key Applications Species Reactivity Qty/Pk
16-218
16-222
05-1336
04-817
05-598
06-570
07-145
04-789
07-424
05-746R
04-746
09-797
07-679
05-1354
17-685
17-10141
AB10029
05-678
05-1312
Anti-Phospho-Histone H3 (Ser10) Antibody, clone 3H10, Cy5 conjugateAnti-Phospho-Histone H3 (Ser10) Antibody, clone 3H10, FITC ConjugateAnti-phospho-Histone H3 (Ser10) Antibody, clone CMA312Anti-phospho-Histone H3 (Ser10) Antibody, clone MC463, rabbit monoclonalAnti-phospho-Histone H3 (Ser10) Antibody, clone RR002Anti-phospho-Histone H3 (Ser10) Antibody, Mitosis MarkerAnti-phospho-Histone H3 (Ser28) AntibodyAnti-phospho-Histone H3 (Thr11) Antibody, clone MC83, rabbit monoclonalAnti-phospho-Histone H3 (Thr3) AntibodyAnti-phospho-Histone H3 (Thr3) Antibody, clone JY325, rabbit monoclonalAnti-phospho-Histone H3 (Thr3) Antibody, clone JY325, rabbit monoclonalAnti-phospho-Histone H3(Ser10) AntibodyAnti-phospho-Histone H3.3 (Ser31) AntibodyAnti-Histone H3 Antibody (Dimethyl K9, Phospho S10), clone 6HH3-2C5ChIPAb+ Phospho-Histone H3 (Ser10) - ChIP Validated Antibody and Primer SetChIPAb+™ Phospho-Histone H3 (Thr3) - ChIP Validated Antibody and Primer Set, rabbit monoclonalAnti-Ubiquityl Histone H2A.X (Lys119) AntibodyAnti-ubiquityl-Histone H2A Antibody, clone E6C5Anti-Ubiquityl-Histone H2B Antibody, clone 56
Immunofluorescence;Western Blotting
Western Blotting;Immunocytochemistry;ImmunofluorescenceWestern Blotting;ELISA;Immunocytochemistry;Multiplexing; ChIPImmunofluorescence;Western Blotting;Immunocytochemistry;ChIP-seq;Dot Blot;Multiplexing; ChIPImmunocytochemistry;Immunohistochemistry;Western BlottingImmunocytochemistry;Immunoprecipitation;Western BlottingWestern Blotting;Immunocytochemistry;Dot Blot;Peptide Inhibition Assay; ChIPWestern Blotting;Peptide Inhibition Assay;Multiplexing
Immunofluorescence;Western Blotting;ImmunocytochemistryWestern Blotting;Peptide Inhibition Assay
Western Blotting;Dot Blot;Multiplexing;Peptide Inhibition Assay
Western Blotting;ELISA;ImmunohistochemistryWestern Blotting;Peptide Inhibition Assay
Western Blotting;Immunocytochemistry
Western Blotting; ChIP
Western Blotting;Dot Blot; ChIP
Western Blotting;Immunoprecipitation;ImmunocytochemistryImmunocytochemistry;Western Blotting; ChIPWestern Blotting; ChIP
Human
Human
Human;Vertebrates
Human
Human;Vertebrates;NematodeMouse;Human
Human;Mouse
Human
Human
Human
Human
Human
Human
Human;Drosophila
Green Plants;Vertebrates
Human
Human;Mouse
Human;Rat;Mouse;Monkey;AmphibianHuman;Mouse;Rat
100 µg
100 µg
100 µg
100 µL
200 µg
200 µg
100 µL
200 µL
100 µL
100 µL
100 µL
400 µL
100 µL
100 µL
25 assays
25 assays
100 µL
200 µL
100 µg
DescriptionName Key Applications Species Reactivity Qty/Pk
经ChIP验证抗体套装(抗体+正对照引物对+负对照抗体)
17-10046
17-675
17-10047
17-615
ChIPAb+ Histone H3 (CT) - ChIP Validated Antibody and Primer Set, rabbit monoclonalChIPAb+ Histone H3 (Unmodified Lys4) - ChIP Validated Antibody and Primer SetChIPAb+ Histone H4 - ChIP Validated Antibody and Primer Set, rabbit monoclonalChIPAb+ Acetyl-Histone H3 - ChIP Validated Antibody and Primer Set
Western Blotting;Immunoprecipitation;Dot Blot; ChIP
Western Blotting; ChIP
Western Blotting; ChIP
Western Blotting; ChIP
Human;Mouse;Rat;Yeast (S. cerevisiae);Chicken
Vertebrates
Human
Human;Mouse;Tetrahymena
25 assays
25 assays
25 assays
25 assays
19
65%的文章使用该抗体进行实验
参考文献:
Human oocytes reprogram adult somatic nuclei of
a type 1 diabetic to diploid pluripotent stem cells.
Yamada, Mitsutoshi, et al.
Nature, 2014 DARPP-32 interaction with adducin
may mediate rapid environmental effects on
striatal neurons. Engmann O, Giralt A, Gervasi N,
Marion-Poll L, Gasmi L, Filhol O, Picciotto MR,
Gilligan D, Greengard P, Nairn AC, Hervé D, Girault
JA. Nat Commun. 2015 Dec 7
DescriptionName Key Applications Species Reactivity Qty/Pk
17-10051
17-683
17-10050
17-658
17-609
17-630
17-10101
17-10045
17-10112
17-10111
17-10241
17-643
17-676
17-680
17-651
17-10108
17-677
17-648
17-681
17-10125
17-10130
ChIPAb+ Acetyl-Histone H3 (Lys14) - ChIP Validated Antibody and Primer Set, rabbit monoclonalChIPAb+ Acetyl-Histone H3 (Lys27) - ChIP Validated Antibody and Primer Set ChIPAb+Acetyl- Histone H3 (Lys4) - ChIP Validated Antibody and Primer SetChIPAb+ Acetyl-Histone H3 (Lys9) Purified - ChIP Validated Antibody and Primer SetChIPAb+ Acetyl-Histone H3 (Lys9) Serum - ChIP Validated Antibody and Primer SetChIPAb+ Acetyl-Histone H4 - ChIP Validated Antibody and Primer SetChIPAb+ Acetyl-Histone H4 (Lys16) - ChIP Validated Antibody and Primer SetChIPAb+ Acetyl-Histone H4 (Lys5) - ChIP Validated Antibody and Primer Set, rabbit monoclonalChIPAb+™ Acetyl Histone H3 (Lys23) - ChIP Validated Antibody and Primer SetChIPAb+™ Acetyl-Histone H3 (Lys18) - ChIP Validated Antibody and Primer SetChIPAb+™ Acetyl-Histone H3 (Lys9/18) - ChIP Validated Antibody and Primer SetChIPAb+ Monomethyl-Histone H3 (Lys27) - ChIP Validated Antibody and Primer Set
ChIPAb+ Monomethyl-Histone H3 (Lys4) - ChIP Validated Antibody and Primer SetChIPAb+ Monomethyl-Histone H3 (Lys9) - ChIP Validated Antibody and Primer SetChIPAb+ Monomethyl-Histone H4 (Lys20) - ChIP Validated Antibody and Primer SetChIPAb+ Dimethyl-Histone H3 (Lys27) - ChIP Validated Antibody and Primer Set, rabbit monoclonalChIPAb+ Dimethyl-Histone H3 (Lys4) - ChIP Validated Antibody and Primer SetChIPAb+ Dimethyl-Histone H3 (Lys9) - ChIP Validated Antibody and Primer SetChIPAb+ Dimethyl-Histone H3 (Lys9) - ChIP Validated Antibody and Primer SetChIPAb+™ Dimethyl-Histone H3 (Lys79) - ChIP Validated Antibody and Primer Set, rabbit monoclonalChIPAb+™ Trimethyl-Histone H3 (Lys79) - ChIP Validated Antibody and Primer Set
ChIP;ChIP-seq;Western Blotting;Immunocytochemistry;Immunoprecipitation;Immunohistochemistry (Paraffin)Western Blotting; ChIP
Western Blotting; ChIP
Western Blotting;Immunocytochemistry; ChIP;ChIP-seq
Western Blotting; ChIP
Western Blotting;Multiplexing;Cell Function Assay; ChIP
Immunoprecipitation; Western Blotting; ChIP
Western Blotting;Immunoprecipitation; ChIP
Immunoprecipitation; Western Blotting; ChIP
Western Blotting;Dot Blot; ChIP; ChIP-seq
Western Blotting; ChIP
Flow Cytometry;Electrophoretic Mobility Shift Assay;Immunofluorescence;Immunohistochemistry;Immunohistochemistry (Paraffin);Western Blotting; ChIPWestern Blotting; ChIP
Western Blotting;ChIP-seq; ChIP
Western Blotting; ChIP
ChIP;Western Blotting;Cell Function Assay
Western Blotting; ChIP; ChIP-seq;Dot Blot;Multiplexing
Western Blotting;Immunoprecipitation; ChIP
Western Blotting; ChIP
Western Blotting; ChIP
Western Blotting; ChIP; Multiplexing
Human;Rat
Vertebrates
Human
Human;Mouse;Mammals
Human;Mouse;Mammals
Human;Eukaryote
Human;Vertebrates
Human
Human;Vertebrates;Yeast (S. cerevisiae)
Human;Vertebrates;Yeast (S. cerevisiae)
Bovine;Human;Mouse;Yeast (S. cerevisiae)
Human;Mouse;Mammals
Vertebrates
Vertebrates
Human;Green Plants;Vertebrates
Vertebrates
Vertebrates
Human;Mouse;Mammals
Vertebrates
Human;Vertebrates
Human;Vertebrates
25 assays
25 assays
25 assays
25 assays
25 assays
25 assays
25 assays
25 assays
25 assays
25 assays
25 assays
25 assays
25 assays
25 assays
25 assays
25 assays
25 assays
25 assays
25 assays
25 assays
25 assays
AB9730
AB9746
AB9732
MABC749
ABE1007AB10410
AB1579407-53507-822
05-797R
05-1152
07-507
MABE329
MABS1190
06-225
MABS15006-47806-659
06-828
05-107607-1333
05-1072
05-1028
MABS64
ABS29
09-272
ABS29
05-747
07-64107-852
07-641
Anti-14-3-3 β Antibody
Anti-14-3-3 ζ Antibody
Anti-14-3-3 ε Antibody, CT
Anti-phospho-BAD (Ser112), clone EPR1891(2), Rabbit Monoclonal AntibodyAnti-BATF3 AntibodyAnti-YES (Proto-oncogene c-Yes) AntibodyAnti-Dexras1 AntibodyAnti-MKP1 AntibodyAnti-phospho-eIF2Bε (Ser539) AntibodyAnti-phospho-Erk1/2 (Thr202/Tyr204, Thr185/Tyr187)Antibody, recombinant clone AW39R, rabbit monoclonalAnti-Erk1/2 (p44/p42) Antibody, clone MK12
Anti-phospho-BMK1/Erk5 (Thr218/Tyr220) AntibodyAnti-c-Fos Antibody, clone 2G9C3Anti-Glucokinase/GCK Antibody, clone 9G1.2 Anti-c-Jun Antibody
Anti-HGK Antibody, clone 2H1.1Anti-HSP27 AntibodyAnti-phospho-c-Jun (Ser73) AntibodyAnti-phospho-c-Jun (Ser63) AntibodyAnti-c-Jun Antibody, clone 6E4.4Anti-JunB Antibody
Anti-Ras Antibody, (K-, H-, N-), clone 9A11.2Anti-phospho-MAPKAPK2 (Thr334) Antibody, clone 9F10.2Anti-phospho-p38 (Thr180/Tyr182) Antibody, clone 6E5.2Anti-p38/SAPK2A Antibody
Anti-phospho-p38α (Thr180/Tyr182) AntibodyAnti-p38/SAPK2A Antibody
Anti-phospho-MEK1 (Ser218/222)/MEK2 (Ser222/226) Antibody, rabbit monoclonalAnti-MEK1 AntibodyAnti-phospho-MEK1 (Thr292) AntibodyAnti-MEK1 Antibody
ELISA;Western Blotting;Immunohistochemistry
ELISA;Western Blotting;Immunohistochemistry
ELISA;Western Blotting;Immunohistochemistry
Western Blotting
Western Blotting;Flow CytometryWestern Blotting
Western BlottingWestern BlottingWestern Blotting
Multiplexing;Western Blotting
Western Blotting;Immunoprecipitation;Immunohistochemistry (Paraffin);ImmunocytochemistryWestern Blotting;Peptide Inhibition Assay
Western Blotting;Immunocytochemistry
Western Blotting;Immunohistochemistry (Paraffin)Immunoprecipitation;Western Blotting;ImmunocytochemistryWestern BlottingWestern Blotting;ImmunohistochemistryImmunocytochemistry;Immunoprecipitation;Western BlottingWestern Blotting
Western Blotting;ImmunocytochemistryImmunocytochemistry;Western Blotting
Western Blotting;Immunohistochemistry (Paraffin)Immunofluorescence;ELISA;Western Blotting
Western Blotting;Immunocytochemistry
Western Blotting
Immunoprecipitation; Western Blotting
Western Blotting
Western Blotting
Immunoprecipitation; Western BlottingWestern Blotting
Immunoprecipitation; Western Blotting
Bovine;Human;Mouse;Rat;Rabbit;SheepBovine;Chicken;Human;Mouse;Rat;Rabbit;SheepBovine;Chicken;Human;Mouse;Rat;Rabbit;SheepHuman
HumanHuman;Horse
MouseHuman;MouseHuman;Rat
Human;Mouse;Rat
Human;Mouse;Rat;Canine;Xenopus;Bovine;Chicken
Human;Mouse;Rat;Bovine
Human
Human
Avian;Human;Mouse
RatHumanHuman;Mouse
Human;Mouse;Rat
Human;Mouse;RatHuman;Mouse;Rat;Canine;Horse;BovineHuman;Mouse;Rat
Human;Mouse;Rat
Human;Mouse
Human;Mouse;Rat;Horse;Rhesus MacaqueHuman;Mouse;Rat
Human;Mouse;Rat;Horse;Rhesus MacaqueHuman;Mouse;Rat
Human;Mouse;RatHuman;Mouse
Human;Mouse;Rat
100 µL
100 µL
100 µL
100 µL
100 µg100 µg
100 µL100 µg100 µL
100 µL
100 µg
200 µL
100 µg
100 µg
200 µL
100 µg250 µg100 µL
100 µL
100 µg100 µg
100 µg
100 µg
100 µg
100 µg
100 µL
100 µg
100 µL
200 µg100 µL
200 µg
20
DescriptionName Key Applications Species Reactivity Qty/Pk
17-622
17-10032
17-614
17-678
17-625
17-671
17-685
17-10131
17-10141
17-650
ChIPAb+ Trimethyl-Histone H3 (Lys27) - ChIP Validated Antibody and Primer SetChIPAb+ Trimethyl-Histone H3 (Lys36) - ChIP Validated Antibody and Primer Set, rabbit monoclonalChIPAb+ Trimethyl-Histone H3 (Lys4) - ChIP Validated Antibody and Primer Set, rabbit monoclonalChIPAb+ Trimethyl-Histone H3 (Lys4) - ChIP Validated Antibody and Primer SetChIPAb+ Trimethyl-Histone H3 (Lys9) - ChIP Validated Antibody and Primer SetChIPAb+ Trimethyl-Histone H4 (Lys20) - ChIP Validated Antibody and Primer Set, rabbit monoclonalChIPAb+ Phospho-Histone H3 (Ser10) - ChIP Validated Antibody and Primer SetChIPAb+™ Phospho-CREB (Ser133) - ChIP Validated Antibody and Primer SetChIPAb+™ Phospho-Histone H3 (Thr3) - ChIP Validated Antibody and Primer Set, rabbit monoclonalChIPAb+ Ubiquityl-Histone H2B - ChIP Validated Antibody and Primer Set
Western Blotting;ChIP
ChIP;Western Blotting;Cell Function Assay;Dot Blot
Western Blotting; ChIP;ChIP-seq
Western Blotting; ChIP;ChIP-seq
Western Blotting;Dot Blot; ChIP
Western Blotting; ChIP
Western Blotting; ChIP
Electrophoretic Mobility Shift Assay;Immunohistochemistry;Immunoprecipitation;Western Blotting; ChIPWestern Blotting;Dot Blot; ChIP
Western Blotting;Immunofluorescence; ChIP
Human;Mouse
Human;Chicken
Human;Mouse;Mammals
Human;Vertebrates
Vertebrates
Human;Green Plants;Vertebrates
Green Plants;Vertebrates
Human;Rat;Mouse;Hamster
Human
Mouse;Human;Vertebrates;Rat;Green Plants
25 assays
25 assays
25 assays
25 assays
25 assays
10 assays
25 assays
25 assays
25 assays
25 assays
07-853
07-69136-010
ABS94
ABC3607-865
07-250
05-577
05-421
07-604
05-389
07-45307-814
05-516
07-147706-918
06-74807-175
05-986MABS771
06-11807-33705-184
04-889
07-791
05-677
07-909
MAB4369
06-133606-1425
Anti-phospho-MEK1 (Thr386) AntibodyAnti-MEKK3 AntibodyAnti-phospho-MKK7/SKK4 (Thr275/Ser277) AntibodyAnti-MLK1 Antibody
Anti-PEA-15 AntibodyAnti-phospho-PED/PEA-15 (Ser116) AntibodyAnti-PP2A Antibody, A subunit
Anti-PP2A Antibody, C subunit, demethylated, clone 4b7Anti-PP2A Antibody, C subunit, clone 1D6
Anti-Rac2 Antibody
Anti-Rac1 Antibody, clone 23A8
Anti-B-Raf AntibodyAnti-phospho-c-Raf (Ser338/Tyr340) AntibodyAnti-Ras Antibody, clone RAS10
Anti-Rsk1 AntibodyAnti-Rsk2/MAPKAP Kinase 1b AntibodyAnti-JNK/SAPK1 AntibodyAnti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) AntibodyAnti-JNK2 AntibodyAnti-JNK3, clone EPR5493(2), Rabbit Monoclonal AntibodyAnti-SHP-2/SHPTP-2 AntibodyAnti-SOS1 Antibody, CTAnti-Src Antibody, clone GD11
Anti-Src Antibody, clone N6L, rabbit monoclonalAnti-phospho-Src Antibody (Tyr215)Anti-phospho-Src (Tyr416) Antibody, clone 9A6Anti-phospho-Src (Tyr418) AntibodyAnti-SRF [Serum Response Factor] Antibody, clone 1E1Anti-TAB1 AntibodyAnti-phospho-TAK1 (Ser412) Antibody
Western Blotting
Western BlottingWestern Blotting
Immunohistochemistry (Paraffin)
Western Blotting;ImmunohistochemistryWestern Blotting
Immunoprecipitation;Western Blotting
Immunocytochemistry;Immunoprecipitation;Western BlottingImmunocytochemistry;Immunoprecipitation;Western Blotting
Immunocytochemistry;Immunoprecipitation;Western BlottingImmunohistochemistry;Immunoprecipitation;Western BlottingImmunoprecipitation;Western BlottingWestern Blotting
ELISA;Flow Cytometry;Immunocytochemistry;Immunohistochemistry;Immunoprecipitation; Western BlottingWestern Blotting;Immunoprecipitation;ELISAImmunoprecipitation;Western Blotting
Immunoprecipitation; Western BlottingWestern Blotting;Enzyme Assay;Immunoprecipitation
ELISA;Western BlottingWestern Blotting;Immunocytochemistry;Flow CytometryWestern Blotting;ImmunocytochemistryImmunoprecipitation; Western BlottingImmunoprecipitation; Western Blotting
Western Blotting
ELISA;Western Blotting
Western Blotting;Immunoprecipitation
Immunocytochemistry;Immunohistochemistry;Western BlottingWestern Blotting;Immunocytochemistry
Western BlottingWestern Blotting
Human;Mouse;Rat
Human;Mouse;RatHuman
Human;Rat;Mouse;Chimpanzee;Rhesus MacaqueHuman;Mouse;RatHuman;Mouse
Human;Mouse;Pig;Rat;XenopusVertebrates
Human;Rat;Mouse;Bovine;Rabbit;Xenopus;Yeast S. cerevisiae
Human;Mouse
Human;Mouse;Rat
Human;Mouse;RatHuman
Human;Rat;Mouse
Human;Mouse;RatHuman;Mouse;Rat
Human;Mouse;RatMouse;Human;Rat;Canine;Xenopus;Chicken;Bovine;HorseHuman;Mouse;RatHuman
Human;MouseHuman;Mouse Avian;Human;Mouse;Mink;Rat
Human
Human
Human;Mouse
Chicken;Human;Mouse
Human;Mouse
Human;Mouse;Rhesus MacaqueHuman;Mouse
100 µL
100 µL100 µL
100 µg
100 µg100 µL
100 µg
200 µL
200 µg
50 µL
250 µg
200 µg100 µL
100 µg
100 µg100 µg
200 µg200 µg
100 µg100 µL
200 µg200 µg200 µg
100 µl
100 µL
100 µg
100 µL
100 µg
100 µg200 µL
21
Vainchenker, W., and Constantinescu, S.N., 2013. Oncogene 32, 2601.Yu, H., et al. 2009. Nat. Rev. Cancer 9, 798.Pfeifer, A.C., et al. 2008. Essays Biochem. 45, 109.Rawlings, J.S. et al. 2004. J. Cell Sci. 117, 1281.Shuai, K., and Liu, B. 2003. Nat. Rev. Immunol. 3, 900.
参考文献
JAK/STAT SignalingJAK/STAT型号通路在细胞生长、分化、迁移及死亡等的基本细胞功能中发挥着中要作用。白细胞中表达
了许多JAK-STAT通路,并因此参与免疫系统的调控。
来自干扰素、白细胞介素、生长因子或其它化学信使的信号可以激活该信号通路受体,从而激活了JAK的激酶功能,导致对其自身的磷酸化(磷酸基团作为蛋白质上的开关);接下来STAT蛋白结合到被磷酸
化的受体上,在此STAT被JAK磷酸化;被磷酸化的STAT蛋白结合到另一个被磷酸化的STAT蛋白上(二聚
化)并易位到细胞核中;在细胞核中,它结合到DNA上并启动转录那些响应STAT的基因。
JAK-STAT途径是进化保守的,从黏菌和蠕虫到哺乳动物(但真菌或植物则不是)。若JAK-STAT的功能被打
乱或失调会导致免疫缺陷综合征及癌症。
Nucleus
Growth Factors
RTK
Growth Hormones
Growth Hormone Receptors
JAKsJAKs
Cytokines GPCR Ligands
Cytokine Receptor GPCR
• AG 490 • JAK2 Inhibitor II • JAK Inhibitor I
• PP2, Indirubin Derivative
• STAT5 Inhibitor
• STAT5 Inhibitor
• STAT3 Inhibitor V, Stattic • STAT3 Inhibitor Peptide, Cell Permeable • STAT3 Inhibitor III, WP1066
• STAT3 Inhibitor V, Stattic • P3-AHNP Peptide Carrier• STAT3 Inhibitor III, WP1066
• STAT3 Inhibitor V, Stattic • P3-AHNP Peptide Carrier• STAT3 Inhibitor III, WP1066
• SHP1/2 PTPase Inhibitor, NSC-87877
• AG 490• JAK2 Inhibitor II,
• JAK Inhibitor I
• AG 490 • JAK2 Inhibitor II • JAK Inhibitor I
• c-Myc Inhibitor
• PD 153035
• LY 294002
• U0126
Akt Pathway
Gene Expression
STAT3STAT5
JAK2
Src
JAKs
Akt
Raf
MEK
ERKs
SHP2JAKs JAKs
Cofactorsc-Myc
CTFs
STATsSTATs
STAT3STAT3
STATs
STATs STATs
STATs
STATsSTATs
STATsSTATs
STATsSTATs
STAT5STAT5
STATsSTATs
JAK2
Sumo SumoSumo
RANKPNA1
STAM
SH2B
PI 3-K
GRB2SOS Ras
Phosphorylation
Calbiochem® Inhibitors
Description Species Reactivity* Applications** Qty/Pk Catalogue No.
Anti-JAK1 H, Ht, M, R, Rb IP, WB 150 µL 06-272
Anti-JAK1 H, M, R IP, WB 100 µL AB3802
Anti-JAK1 H, M, R WB 100 µg 07-1485
Anti-JAK1, clone 73 H, M, R, Ca, Xn, Ch IF, IP, WB 100 µg 05-1154
Anti-JAK2 H, M, R IP, WB 150 µL 06-255
Anti-JAK2 H, M, R IP, WB 100 µg 06-1310
Anti-JAK2, Agarose conjugate H, M, R IP 200 µg 16-121
Anti-JAK2, clone 8E10.2 H, M, R IH(P), WB 100 µL 04-001
Anti-phospho-JAK2 (Tyr317) H WB 100 µL 07-1318
JAK/STAT 信号通路研究抗体
参考文献:
Virally infected and matured human dendritic cells
activate natural killer cells via cooperative activity
of plasma membrane-bound TNF and IL-15.
Blood 116 575-83. Epub 2010 Apr 29. 2010
The mitogen-activated protein kinase and JAK-STAT
signaling pathways are required for an oncostatin
M-responsive element-mediated activation of
matrix metalloproteinase 1 gene expression. J. Biol.
Chem., 272: 1188-96 (1997) 1997
22
Anti-phospho JAK2 (Tyr570) H, R, Eq, Chp, Pm, Po
WB 100 µg 09-241
Anti-phospho JAK2 (Tyr813) H, M, R, Eq, Chp, Po WB 100 µg 09-242
Anti-phospho JAK2 (Tyr972) H, R, Eq, Op, B, Chp, Po
WB 100 µg 09-243
Anti-phospho-JAK2 (Tyr1007/1008) H, Ch, M, Po, R, Xn, Zebra�sh
WB 200 µL 09-275
Anti-phospho-JAK2 (Tyr1007/1008) H, M, R WB 200 µg 07-606
Anti-phospho-JAK2 (Tyr1007/Tyr1008), clone E132 H, M, R ICC, IH(P), IP, WB 100 µL 04-1098
Anti-Phospho-JAK1 (TYR1022/1023) H, M, R WB 100 µL 07-1489
Anti-JAK3 M WB 150 µL 06-342
Anti-JAK3 H, M IP, WB 100 µL 07-1487
Anti-JAK3, clone B32-32 H, M IP 200 µg 05-406
Anti-JAK3, clone HL423 H, M Mplex, WB 200 µL 04-011
Anti-JAK3, CT H WB 250 µg 06-667
Anti-STAT1 H WB 100 µL 04-069
Anti-STAT1, CT H, M EMSA, IP, WB 250 µg 06-501
Anti-STAT1-α H, M IP, WB 100 µg AB16951
Anti-Phospho-STAT1 (Tyr701), clone 5C9.2 H, M, R WB 100 µg 05-1064
参考文献:
JAK2, but not Src family kinases, is required for
STAT, ERK, and Akt signaling in response to growth
hormone in preadipocytes and hepatoma cells.
Molecular endocrinology (Baltimore, Md.) 22
1825-41, 2008
alpha-Syntrophin regulates ARMS localization at
the neuromuscular junction and enhances EphA4
signaling in an ARMS-dependent manner. J Cell
Biol, 169:813-24, 2005
参考文献:
c-Abl Activates Janus Kinase 2 in Normal
Hematopoietic Cells.doi: 10.1074/jbc.M114.554501
A Novel Role for Histone Deacetylase 6 in the
Regulation of the Tolerogenic STAT3/IL-10 Pathway
in APCs.doi: 10.4049/ jimmunol.1302778
JAK/STAT 信号通路研究抗体
Description Species Reactivity* Applications** Qty/Pk Catalogue No.
Anti-phospho-STAT1 (Tyr701) Ch, H, M, R WB 100 µL 07-307
Anti-phospho-STAT1 (Tyr701), Alexa Fluor® 488 conjugated, clone 5C9.2
H, M, R FC, WB 100 tests FCMAB106A4
Anti-phospho-STAT1 (Ser727) H, M WB 200 µL 07-714
Anti-phospho-STAT1 (Ser 727), clone 12C5 H ELISA, WB 100 µg 04-478
Anti-STAT2 M ICC, EMSA, IP, WB 200 µg 07-140
Anti-STAT2, CT H, M ICC, EMSA, IP, WB 200 µg 06-502
Anti-phospho-STAT2 (Tyr689) H, M IP, WB 100 µL ABE541
Anti-phospho-STAT2 (Tyr689) H, M IP, WB 200 µL 07-224
Anti-STAT3 H, M, R ICC, EMSA, IP, WB 200 µg 06-596
Anti-STAT3, clone 18F7.1 H, M ICC, WB 100 µg 05-1078
Anti-STAT3, clone E121-21 H ICC, IH(P), IP, WB 100 µL 04-1014
Anti-phospho-STAT3 (Tyr68) Ch, B, H, M, Po, R, Xn
WB 100 µg 07-1347
Anti-phospho-STAT3 (Tyr705), clone 9E12 Ca, H, M ELISA, IP, WB 100 µg 05-485
Anti-phospho-STAT3 (Ser727) M WB 100 µL 07-703
Anti-STAT5A H, M, R IP, WB 100 µL 06-968
Anti-phospho-STAT5A/B (Ser726/Ser731) H, M, R WB 200 µL 06-867
Anti-STAT5A/B H, M, R, Sh EMSA 250 µL 06-588
Anti-STAT5B H, M WB 200 µg 06-554
Anti-STAT5B Av, H, M, R IP, WB 100 µL 06-969
Anti-STAT5α (CT), clone E289 H, R ICC, FC, IH(P), IP, WB 100 µL 04-1016
Anti-phospho-STAT5A/B (Tyr694/699), clone 8-5-2
B, H, M, R, Sh WB 100 µg 05-495
Anti-phospho-STAT5A/B (Tyr694/699) B, H, M, R DB, WB 100 µL 07-586
Anti-phospho-STAT5A/B (Tyr694/699), clone A11W
B, Chp, H, M, R WB (Not IP) 100 µL 04-886
Anti-phospho-STAT5A/B (Tyr694/699), clone A11W H, M WB 100 µL 05-886
参考文献:
High STAT1 mRNA levels but not its tyrosine
phosphorylation are associated with macrophage
in�ltration and bad prognosis in breast cancer.
BMC Cancer 2014, 14:257
STAT2 de�ciency and susceptibility to viral illness in
humans.PNAS, February 19, 2013
Description Species Reactivity* Applications** Qty/Pk Catalogue No.
23
*Species: Av: avian; B: bovine, Ca: canine; Ch: chicken; Chp: chimpanzee; Eq: equine; H: human; M: mouse, Op: opossum; Pm: primate;R: rat; Sh: sheep; Xn: xenopus**Applications: ELISA: enzyme-linked immunosorbent assay; EMSA: electrophoretic mobility shift assay; FC: �ow cytometry; ICC: immunocytochemistry,IF: immuno�uorescence; IH: immunohistochemistry; IP: immunoprecipitation; Mplex: multiplexing; WB: Western blotting
JAK 抑制剂Description Catalogue No. Comments Size
JAK Inhibitor I (Pyridone 6) 420099 A cell-permeable, reversible, ATP-competitive inhibitor of JAK1 (IC50 = 15 nM for murine JAK1), JAK2 (IC50 = 1 nM), JAK3 (Ki = 5 nM), and Tyk2 (IC50 = 1 nM).
500 µg 1 mg
InSolution™ JAK Inhibitor I 420097 A 10 mM (500 µg/162 µL) solution of JAK Inhibitor I (Cat. No. 420099) in DMSO.
500 µg
JAK2 Inhibitor II 1,2,3,4,5,6-Hexabromo-cyclohexane
420132 A cell-permeable, speci�c, reversible, and direct inhibitor of JAK2 autophosphorylation (maximal inhibition at 50 µM in BSC-40 cells overexpressing JAK2).
25 mg
JAK2 Inhibitor III, SD-1029 NSC 371488
573098 A cell-permeable, selective inhibitor of JAK2. 10 mg
JAK2 Inhibitor IV 3-Amino-5-(N-tert-butyl-sulfonamido-4-phenyl)-indazole
420139 A potent inhibitor of both the wild-type JAK2 and the constitutively active V617F mutant (IC50 = 78 and 206 nM, respectively).
5 mg
JAK2 Inhibitor IX, WP1193 420151 A cell-permeable analog of WP1130 (Cat. No. 681685) that inhibits JAK2 kinase activity and suppresses JAK2-dependent STAT3 Tyr705 phosphorylation.
10 mg
JAK2 Inhibitor V, Z3 2-Methyl-1-phenyl-4-pyridin-2-yl-2-(2-pyridin-2-ylethyl)butan-1-one
420141 A cell-permeable inhibitor of autophosphorylation of both the wild-type JAK2 and the constitutively active V167F mutant (IC50 = 15 and 28 µM, respectively).
10 mg
JAK3 Inhibitor I WHI-P131 420101 A cell-permeable, reversible, potent, ATP-competitive, and speci�c inhibitor of JAK3 (IC50 = 78 µM).
5 mg
JAK3 Inhibitor II WHI-P154
420104 A potent, cell-permeable, reversible, ATP-competitive, and speci�c inhibitor of JAK3 with no effect on JAK1 and JAK2.
5 mg
JAK3 Inhibitor, Negative Control; WHI-P258, HCl
420112 A useful negative control for JAK3 Inhibitors (IC50 > 300 µM).
1 mg
JAK3 Inhibitor IV ZM 39923
420121 A potent, selective, and ATP-competitive inhibitor of Janus tyrosine kinase-3 (JAK3) (pIC50 = 7.1).
10 mg
JAK3 Inhibitor V ZM 449829
420122 A breakdown product of JAK3 Inhibitor IV (Cat. No. 420121) with similar inhibitory potency (pIC50 = 6.8 for JAK3)
10 mg
JAK3 Inhibitor VI 420126 A cell-permeable, potent, and reversible inhibitor of JAK3 (IC50 = 27 nM).
5 mg
JAK3 Inhibitor VII, AD412 N-(Pyridin-4-yl)-3-[1-(4-chloro- benzyl)indol-3-yl]-propanamide
420145 A cell-permeable, selective inhibitor of JAK3 (by 81% and 36% at 90 and 30 µM, respectively)
25 mg
JAK3 Inhibitor VIII NSC114792 420146 A cell-permeable compound that targets JAK3 kinase domain and selectively inhibits JAK3, but not JAK1, JAK2, or TYK2.
10 mg
Anti-phospho STAT6 (Tyr641), clone 19E2.1 H, M FC, WB 100 µg MABE270
Anti-phospho-STAT6 (Tyr641), clone 16E12 H, M, R WB 100 µg 05-590
Anti-phospho-STAT6 (Tyr641) H, M WB 100 µL 06-937
Description Species Reactivity* Applications** Qty/Pk Catalogue No.
24
STAT 抑制剂Description Catalogue No. Comments Size
STAT3 Inhibitor Peptide Ac-PpYLKTK-OH
573095 A STAT3-SH2 domain binding phosphopeptide that acts as a selective inhibitor of STAT3 signaling (DB50 = 235 µM).
1 mg
STAT3 Inhibitor Peptide, Cell-Permeable PpYLKTK-mts
573096 A cell-permeable analog of the STAT3-SH2 domain-binding phosphopeptide (Cat. No. 573095) that contains a C-terminal membrane translocating sequence (MTS).
1 mg 5 mg
STAT3 Inhibitor III, WP1066 573097 A cell-permeable analog of AG 490 (Cat. No. 658401) that acts as a STAT3 pathway inhibitor. Inhibits malignant glioma growth (IC50 = 5.6 and 3.7 µM in U87-MG and U373-MG cells, respectively).
10 mg
InSolution™ STAT3 Inhibitor III, WP1066
573129 A 50 mM (5 mg/281 µL) solution of STAT3 Inhibitor III, WP1066 (Cat. No. 573097) in DMSO.
5 mg
STAT3 Inhibitor V, Stattic 6-Nitrobenzo[b]thiophene-1, 1-dioxide
573099 A cell-permeable inhibitor of STAT3 cellular function that targets the STAT3-SH2 domain and prevents its association with upstream kinases.
25 mg
STAT3 Inhibitor VI, S3I-201 NSC 74859
573102 A cell-permeable compound that binds STAT3-SH2 domain and prevents STAT3 phosphorylation/activation, dimerization, DNA-binding, and STAT3-dependent transcription.
10 mg 25 mg
InSolution™ STAT3 Inhibitor VI, S3I-201
573130 A 50 mM (5 mg/274 µL) solution of STAT3 Inhibitor VI, S3I-201 (Cat. No. 573102) in DMSO.
5 mg
STAT3 Inhibitor VII Ethyl-1-(4-cyano-2,3,5,6-tetra-�uorophenyl)-6,7,8-tri�uoro-4-oxo-1,4-dihydroquinoline-3-carboxylate
573103 A cell-permeable, potent inhibitor of JAK1/JAK2/TYK2/STAT3 activation (IC50 = 170 nM against U266 constitutive STAT3 Y705 phosphorylation).
5 mg
STAT3 Inhibitor VIII, 5,15-DPP 5,15-Diphenylporphyrin
573109 A cell-permeable compound that directly binds to STAT3 and prevents its dimerization. Acts as a STAT3-SH2 domain antagonist (IC50 = 280 nM).
25 mg
STAT3 Inhibitor X, HJB 17-Hydroxy-jolkinolide B
573106 A highly selective inhibitor of JAK/STAT3 signaling with anti-tumor properties (~12 µM). Suppresses IL-6-induced STAT-3 transcription activity (IC50= ˜ 3.34 µM in HepG2 cells).
1 mg
STAT3 Inhibitor Peptide, Cell-permeable, Inactive Control PYLKTK-mts
573105 A cell-permeable, non-phosphorylated inactive control peptide for STAT3 cellular function studies.
5 mg
STAT3 Inhibitor IX, Cpd188 4-((3-(Carboxymethylsulfanyl)-4-hydroxy-1-naphthyl)sulfamoyl)-benzoic acid
573125 A cell-permeable compound that inhibits IL-6-stimulated STAT3 Tyr705 phosphorylation (IC50 = 73 µM) and nuclear translocation (IC50 = 39 µM) in HepG2 cells.
10 mg
STAT3 Inhibitor XI, STX-0119 N-(5-(Furan-2-yl)-1,3,4-oxadiazol-2-yl)-2-phenylquinoline-4-carboxamide
573126 A cell-permeable, selective inhibitor of STAT3 DNA binding activity. Prevents IL-6-stimulated STAT3 dimerization in HEK293 cells (IC50 = 74 µM).
25 mg
STAT3 Inhibitor XII, SPI H2N-FISKERERAILSTKPP-GTFLLRFSESSK-CO2H
573127 A cell-permeable STAT3-SH2 domain-derived (588-615) 28-mer peptide that competitively prevents STAT3 binding to cognate pTyr peptide motif, represses STAT3 dimerization and induces apoptosis.
5 mg
STAT3 Inhibitor XIII, C188-9 F1113-0789
573128 A cell-permeable binaphthol-sulfonamide that competes against pY proteins for STAT3-SH2 domain binding.
10 mg
STAT3 Inhibitor XIV, LLL12 5-Hydroxy-9,10-dioxo-9,10-dihydroanthracene-1-sulfonamide
573131 A cell-permeable compound that binds directly to the phosphoryl Tyr705 binding site of the STAT3 monomer and acts as selective and reversible inhibitor of STAT3 signaling.
10 mg
STAT3 Inhibitor XVIII, BP-1-102 573132 A cell-permeable compound that selectively binds the three subpockets of STAT3- SH2 domain surface (KD = 504 nM) and blocks STAT3 phosphorylation, dimerization, and DNA-binding.
10 mg
STAT5 Inhibitor II, IQDMA N1-(11H-indolo[3,2-c]quinolin-6-yl)-N2,N2-dimethylethane-1,2-diamine
420294 A cell-permeable inhibitor of STAT5 signaling in HL-60 and K562 cells and inhibits the growth of various tumor cells (IC50 = 8, 5, and 8 µM for A549, HL-60, and K562 cells, respectively).
10 mg
25
STAT5 Inhibitor N‘-((4-Oxo-4H-chromen-3-yl)methylene)nicotinohydrazide
573108 A cell-permeable compound that selectively targets the SH2 domain of STAT5 (IC50 = 47 µM against STAT5b SH2 domain EPO peptide binding activity).
10 mg
STAT5 Inhibitor III, Pimozide 573110 A cell-permeable compound that inhibits the constitutive STAT5 Tyr694 phosphorylation and transcription activity in Bcr-Abl+ K562 and KU812 cultures.
100 mg
STAT3/STAT5 DualInhibitor, SH-4-54 509105 A cell-permeable, salicyclic-to-benzoic acid-substituted BP-1-102 (Cat. No. 573132) analog that targets STAT3 and STAT5 with comparable af�nity (koff/kon = KD = 300 and 464 nM, respectively).
10 mg
Description Qty/Pk Catalogue No.
PD 153035 1 mg 234490
Indirubin Derivative E804 1 mg 402081
JAK Inhibitor I 500 µg 420099
JAK2 Inhibitor II 25 mg 420132
LY 294002 5 mg 440202
PP2 1 mg 529573
SHP1/2 PTPase Inhibitor, NSC-87877 50 mg 565851
STAT3 Inhibitor Peptide, Cell Permeable 1 mg 573096
STAT3 Inhibitor III, WP1066 10 mg 573097
STAT3 Inhibitor V, Stattic 25 mg 573099
STAT5 Inhibitor 10 mg 573108
AG490 5 mg 658401
U0126 1 mg 662005
DMSO 15 mL KP31817
JAK/STAT 信号通路抑制剂套装(Catalogue No. 420138)
The panel contains the following inhibitors and one vial of high quality DMSO.
Description Applications Qty/Pk Catalogue No.
JAK1, RNA probe, Hu, Cy3 RNA Expression 250 rxns SF-1288
JAK1, RNA probe, Hu, Cy5 RNA Expression 250 rxns SF-728
JAK2, RNA probe, Hu, Cy3 RNA Expression 250 rxns SF-1410
JAK2, RNA probe, Hu, Cy5 RNA Expression 250 rxns SF-406
JAK3, RNA probe, Hu, Cy3 RNA Expression 250 rxns SF-905
JAK3, RNA probe, Hu, Cy5 RNA Expression 250 rxns SF-476
Description Applications Qty/Pk Catalogue No.
STAT1 Activation Dual Detection Kit Flow Cytometry 25 tests FCCS025142
Phospho STAT1(Tyr701) Milliplex Assay Multiplexed ELISA 96 well 46-655MAG
Total STAT1 Milliplex Assay Multiplexed ELISA 96 well 46-654MAG
JAK/STAT RNA 检测
JAK/STAT 试剂盒
Description Catalogue No. Comments Size
26
17-10046
17-675
17-10047
17-615
ChIPAb+ Histone H3 (CT) - ChIP Validated Antibody and Primer Set, rabbit monoclonalChIPAb+ Histone H3 (Unmodified Lys4) - ChIP Validated Antibody and Primer SetChIPAb+ Histone H4 - ChIP Validated Antibody and Primer Set, rabbit monoclonalChIPAb+ Acetyl-Histone H3 - ChIP Validated Antibody and Primer Set
Western Blotting;Immunoprecipitation;Dot Blot; ChIP
Western Blotting; ChIP
Western Blotting; ChIP
Western Blotting; ChIP
Human;Mouse;Rat;Yeast (S. cerevisiae);Chicken
Vertebrates
Human
Human;Mouse;Tetrahymena
25 assays
25 assays
25 assays
25 assays
MAPK Signaling丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)是一类进化保守性很高的蛋白丝/苏氨酸激酶。因细胞受到细胞因
子、神经递质、激素、细胞应激及细胞黏附等胞外刺激时产生。结合其他几种信号通路一起调节一些转录因子的磷酸化状态。
该信号通路调节着细胞的生长、分化、对环境的应激适应、炎症反应等多种重要的细胞生理/病理过程。MAPK活性异常会导致
肿瘤的发生。
DescriptionName Key Applications Species Reactivity Qty/Pk
抗体
AB9730
AB9746
AB9732
MABC749
ABE1007AB10410
AB1579407-53507-822
05-797R
05-1152
07-507
MABE329
MABS1190
06-225
MABS15006-47806-659
06-828
05-107607-1333
05-1072
05-1028
MABS64
ABS29
09-272
ABS29
05-747
07-64107-852
07-641
Anti-14-3-3 β Antibody
Anti-14-3-3 ζ Antibody
Anti-14-3-3 ε Antibody, CT
Anti-phospho-BAD (Ser112), clone EPR1891(2), Rabbit Monoclonal AntibodyAnti-BATF3 AntibodyAnti-YES (Proto-oncogene c-Yes) AntibodyAnti-Dexras1 AntibodyAnti-MKP1 AntibodyAnti-phospho-eIF2Bε (Ser539) AntibodyAnti-phospho-Erk1/2 (Thr202/Tyr204, Thr185/Tyr187)Antibody, recombinant clone AW39R, rabbit monoclonalAnti-Erk1/2 (p44/p42) Antibody, clone MK12
Anti-phospho-BMK1/Erk5 (Thr218/Tyr220) AntibodyAnti-c-Fos Antibody, clone 2G9C3Anti-Glucokinase/GCK Antibody, clone 9G1.2 Anti-c-Jun Antibody
Anti-HGK Antibody, clone 2H1.1Anti-HSP27 AntibodyAnti-phospho-c-Jun (Ser73) AntibodyAnti-phospho-c-Jun (Ser63) AntibodyAnti-c-Jun Antibody, clone 6E4.4Anti-JunB Antibody
Anti-Ras Antibody, (K-, H-, N-), clone 9A11.2Anti-phospho-MAPKAPK2 (Thr334) Antibody, clone 9F10.2Anti-phospho-p38 (Thr180/Tyr182) Antibody, clone 6E5.2Anti-p38/SAPK2A Antibody
Anti-phospho-p38α (Thr180/Tyr182) AntibodyAnti-p38/SAPK2A Antibody
Anti-phospho-MEK1 (Ser218/222)/MEK2 (Ser222/226) Antibody, rabbit monoclonalAnti-MEK1 AntibodyAnti-phospho-MEK1 (Thr292) AntibodyAnti-MEK1 Antibody
ELISA;Western Blotting;Immunohistochemistry
ELISA;Western Blotting;Immunohistochemistry
ELISA;Western Blotting;Immunohistochemistry
Western Blotting
Western Blotting;Flow CytometryWestern Blotting
Western BlottingWestern BlottingWestern Blotting
Multiplexing;Western Blotting
Western Blotting;Immunoprecipitation;Immunohistochemistry (Paraffin);ImmunocytochemistryWestern Blotting;Peptide Inhibition Assay
Western Blotting;Immunocytochemistry
Western Blotting;Immunohistochemistry (Paraffin)Immunoprecipitation;Western Blotting;ImmunocytochemistryWestern BlottingWestern Blotting;ImmunohistochemistryImmunocytochemistry;Immunoprecipitation;Western BlottingWestern Blotting
Western Blotting;ImmunocytochemistryImmunocytochemistry;Western Blotting
Western Blotting;Immunohistochemistry (Paraffin)Immunofluorescence;ELISA;Western Blotting
Western Blotting;Immunocytochemistry
Western Blotting
Immunoprecipitation; Western Blotting
Western Blotting
Western Blotting
Immunoprecipitation; Western BlottingWestern Blotting
Immunoprecipitation; Western Blotting
Bovine;Human;Mouse;Rat;Rabbit;SheepBovine;Chicken;Human;Mouse;Rat;Rabbit;SheepBovine;Chicken;Human;Mouse;Rat;Rabbit;SheepHuman
HumanHuman;Horse
MouseHuman;MouseHuman;Rat
Human;Mouse;Rat
Human;Mouse;Rat;Canine;Xenopus;Bovine;Chicken
Human;Mouse;Rat;Bovine
Human
Human
Avian;Human;Mouse
RatHumanHuman;Mouse
Human;Mouse;Rat
Human;Mouse;RatHuman;Mouse;Rat;Canine;Horse;BovineHuman;Mouse;Rat
Human;Mouse;Rat
Human;Mouse
Human;Mouse;Rat;Horse;Rhesus MacaqueHuman;Mouse;Rat
Human;Mouse;Rat;Horse;Rhesus MacaqueHuman;Mouse;Rat
Human;Mouse;RatHuman;Mouse
Human;Mouse;Rat
100 µL
100 µL
100 µL
100 µL
100 µg100 µg
100 µL100 µg100 µL
100 µL
100 µg
200 µL
100 µg
100 µg
200 µL
100 µg250 µg100 µL
100 µL
100 µg100 µg
100 µg
100 µg
100 µg
100 µg
100 µL
100 µg
100 µL
200 µg100 µL
200 µg
27
DescriptionName Key Applications Species Reactivity Qty/Pk
抗体
AB9730
AB9746
AB9732
MABC749
ABE1007AB10410
AB1579407-53507-822
05-797R
05-1152
07-507
MABE329
MABS1190
06-225
MABS15006-47806-659
06-828
05-107607-1333
05-1072
05-1028
MABS64
ABS29
09-272
ABS29
05-747
07-64107-852
07-641
Anti-14-3-3 β Antibody
Anti-14-3-3 ζ Antibody
Anti-14-3-3 ε Antibody, CT
Anti-phospho-BAD (Ser112), clone EPR1891(2), Rabbit Monoclonal AntibodyAnti-BATF3 AntibodyAnti-YES (Proto-oncogene c-Yes) AntibodyAnti-Dexras1 AntibodyAnti-MKP1 AntibodyAnti-phospho-eIF2Bε (Ser539) AntibodyAnti-phospho-Erk1/2 (Thr202/Tyr204, Thr185/Tyr187)Antibody, recombinant clone AW39R, rabbit monoclonalAnti-Erk1/2 (p44/p42) Antibody, clone MK12
Anti-phospho-BMK1/Erk5 (Thr218/Tyr220) AntibodyAnti-c-Fos Antibody, clone 2G9C3Anti-Glucokinase/GCK Antibody, clone 9G1.2 Anti-c-Jun Antibody
Anti-HGK Antibody, clone 2H1.1Anti-HSP27 AntibodyAnti-phospho-c-Jun (Ser73) AntibodyAnti-phospho-c-Jun (Ser63) AntibodyAnti-c-Jun Antibody, clone 6E4.4Anti-JunB Antibody
Anti-Ras Antibody, (K-, H-, N-), clone 9A11.2Anti-phospho-MAPKAPK2 (Thr334) Antibody, clone 9F10.2Anti-phospho-p38 (Thr180/Tyr182) Antibody, clone 6E5.2Anti-p38/SAPK2A Antibody
Anti-phospho-p38α (Thr180/Tyr182) AntibodyAnti-p38/SAPK2A Antibody
Anti-phospho-MEK1 (Ser218/222)/MEK2 (Ser222/226) Antibody, rabbit monoclonalAnti-MEK1 AntibodyAnti-phospho-MEK1 (Thr292) AntibodyAnti-MEK1 Antibody
ELISA;Western Blotting;Immunohistochemistry
ELISA;Western Blotting;Immunohistochemistry
ELISA;Western Blotting;Immunohistochemistry
Western Blotting
Western Blotting;Flow CytometryWestern Blotting
Western BlottingWestern BlottingWestern Blotting
Multiplexing;Western Blotting
Western Blotting;Immunoprecipitation;Immunohistochemistry (Paraffin);ImmunocytochemistryWestern Blotting;Peptide Inhibition Assay
Western Blotting;Immunocytochemistry
Western Blotting;Immunohistochemistry (Paraffin)Immunoprecipitation;Western Blotting;ImmunocytochemistryWestern BlottingWestern Blotting;ImmunohistochemistryImmunocytochemistry;Immunoprecipitation;Western BlottingWestern Blotting
Western Blotting;ImmunocytochemistryImmunocytochemistry;Western Blotting
Western Blotting;Immunohistochemistry (Paraffin)Immunofluorescence;ELISA;Western Blotting
Western Blotting;Immunocytochemistry
Western Blotting
Immunoprecipitation; Western Blotting
Western Blotting
Western Blotting
Immunoprecipitation; Western BlottingWestern Blotting
Immunoprecipitation; Western Blotting
Bovine;Human;Mouse;Rat;Rabbit;SheepBovine;Chicken;Human;Mouse;Rat;Rabbit;SheepBovine;Chicken;Human;Mouse;Rat;Rabbit;SheepHuman
HumanHuman;Horse
MouseHuman;MouseHuman;Rat
Human;Mouse;Rat
Human;Mouse;Rat;Canine;Xenopus;Bovine;Chicken
Human;Mouse;Rat;Bovine
Human
Human
Avian;Human;Mouse
RatHumanHuman;Mouse
Human;Mouse;Rat
Human;Mouse;RatHuman;Mouse;Rat;Canine;Horse;BovineHuman;Mouse;Rat
Human;Mouse;Rat
Human;Mouse
Human;Mouse;Rat;Horse;Rhesus MacaqueHuman;Mouse;Rat
Human;Mouse;Rat;Horse;Rhesus MacaqueHuman;Mouse;Rat
Human;Mouse;RatHuman;Mouse
Human;Mouse;Rat
100 µL
100 µL
100 µL
100 µL
100 µg100 µg
100 µL100 µg100 µL
100 µL
100 µg
200 µL
100 µg
100 µg
200 µL
100 µg250 µg100 µL
100 µL
100 µg100 µg
100 µg
100 µg
100 µg
100 µg
100 µL
100 µg
100 µL
200 µg100 µL
200 µg
07-853
07-69136-010
ABS94
ABC3607-865
07-250
05-577
05-421
07-604
05-389
07-45307-814
05-516
07-147706-918
06-74807-175
05-986MABS771
06-11807-33705-184
04-889
07-791
05-677
07-909
MAB4369
06-133606-1425
Anti-phospho-MEK1 (Thr386) AntibodyAnti-MEKK3 AntibodyAnti-phospho-MKK7/SKK4 (Thr275/Ser277) AntibodyAnti-MLK1 Antibody
Anti-PEA-15 AntibodyAnti-phospho-PED/PEA-15 (Ser116) AntibodyAnti-PP2A Antibody, A subunit
Anti-PP2A Antibody, C subunit, demethylated, clone 4b7Anti-PP2A Antibody, C subunit, clone 1D6
Anti-Rac2 Antibody
Anti-Rac1 Antibody, clone 23A8
Anti-B-Raf AntibodyAnti-phospho-c-Raf (Ser338/Tyr340) AntibodyAnti-Ras Antibody, clone RAS10
Anti-Rsk1 AntibodyAnti-Rsk2/MAPKAP Kinase 1b AntibodyAnti-JNK/SAPK1 AntibodyAnti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) AntibodyAnti-JNK2 AntibodyAnti-JNK3, clone EPR5493(2), Rabbit Monoclonal AntibodyAnti-SHP-2/SHPTP-2 AntibodyAnti-SOS1 Antibody, CTAnti-Src Antibody, clone GD11
Anti-Src Antibody, clone N6L, rabbit monoclonalAnti-phospho-Src Antibody (Tyr215)Anti-phospho-Src (Tyr416) Antibody, clone 9A6Anti-phospho-Src (Tyr418) AntibodyAnti-SRF [Serum Response Factor] Antibody, clone 1E1Anti-TAB1 AntibodyAnti-phospho-TAK1 (Ser412) Antibody
Western Blotting
Western BlottingWestern Blotting
Immunohistochemistry (Paraffin)
Western Blotting;ImmunohistochemistryWestern Blotting
Immunoprecipitation;Western Blotting
Immunocytochemistry;Immunoprecipitation;Western BlottingImmunocytochemistry;Immunoprecipitation;Western Blotting
Immunocytochemistry;Immunoprecipitation;Western BlottingImmunohistochemistry;Immunoprecipitation;Western BlottingImmunoprecipitation;Western BlottingWestern Blotting
ELISA;Flow Cytometry;Immunocytochemistry;Immunohistochemistry;Immunoprecipitation; Western BlottingWestern Blotting;Immunoprecipitation;ELISAImmunoprecipitation;Western Blotting
Immunoprecipitation; Western BlottingWestern Blotting;Enzyme Assay;Immunoprecipitation
ELISA;Western BlottingWestern Blotting;Immunocytochemistry;Flow CytometryWestern Blotting;ImmunocytochemistryImmunoprecipitation; Western BlottingImmunoprecipitation; Western Blotting
Western Blotting
ELISA;Western Blotting
Western Blotting;Immunoprecipitation
Immunocytochemistry;Immunohistochemistry;Western BlottingWestern Blotting;Immunocytochemistry
Western BlottingWestern Blotting
Human;Mouse;Rat
Human;Mouse;RatHuman
Human;Rat;Mouse;Chimpanzee;Rhesus MacaqueHuman;Mouse;RatHuman;Mouse
Human;Mouse;Pig;Rat;XenopusVertebrates
Human;Rat;Mouse;Bovine;Rabbit;Xenopus;Yeast (<italic>S. cerevisiae</italic>)Human;Mouse
Human;Mouse;Rat
Human;Mouse;RatHuman
Human;Rat;Mouse
Human;Mouse;RatHuman;Mouse;Rat
Human;Mouse;RatMouse;Human;Rat;Canine;Xenopus;Chicken;Bovine;HorseHuman;Mouse;RatHuman
Human;MouseHuman;MouseAvian;Human;Mouse;Mink;RatHuman
Human
Human;Mouse
Chicken;Human;Mouse
Human;Mouse
Human;Mouse;Rhesus MacaqueHuman;Mouse
100 µL
100 µL100 µL
100 µg
100 µg100 µL
100 µg
200 µL
200 µg
50 µL
250 µg
200 µg100 µL
100 µg
100 µg100 µg
200 µg200 µg
100 µg100 µL
200 µg200 µg200 µg
100 µl
100 µL
100 µg
100 µL
100 µg
100 µg200 µL
28
DescriptionName Key Applications Species Reactivity Qty/Pk
抗体
InhibitorSelect™MAK Kinase Signaling Pathway Inhibitor Panel,目录号444189
组分 靶标 包装量 目录号
ERK InhibitorⅡ,FR180204
JNK InhibitorⅡJNK Inhibitor IXMEK1/2 Inhibitor MNK1 InhibitorMK2a Inhibitorp38 MAP Kinase Inhibitoy VPD 98059Raf Kinase Inhibitor ⅣSB 203580 p38TpI2 Kinase InhibitorZM 336372DMSO
ERK1,ERK2JNK JNK2,JNK3MEK1/2MNK1MK2ap38,CK1MEKB-RafMAPKTpI2 Kinasec-Raf-
1 mg5 mg5 mg1 mg5 mg5 mg1 mg5 mg1 mg1 mg1 mg1 mg15 mL
328007420119420136444939454861475863506156513000553014559389616373692000KP31817
*以上组分可按目录号单独购买
抑制剂
07-853
07-69136-010
ABS94
ABC3607-865
07-250
05-577
05-421
07-604
05-389
07-45307-814
05-516
07-147706-918
06-74807-175
05-986MABS771
06-11807-33705-184
04-889
07-791
05-677
07-909
MAB4369
06-133606-1425
Anti-phospho-MEK1 (Thr386) AntibodyAnti-MEKK3 AntibodyAnti-phospho-MKK7/SKK4 (Thr275/Ser277) AntibodyAnti-MLK1 Antibody
Anti-PEA-15 AntibodyAnti-phospho-PED/PEA-15 (Ser116) AntibodyAnti-PP2A Antibody, A subunit
Anti-PP2A Antibody, C subunit, demethylated, clone 4b7Anti-PP2A Antibody, C subunit, clone 1D6
Anti-Rac2 Antibody
Anti-Rac1 Antibody, clone 23A8
Anti-B-Raf AntibodyAnti-phospho-c-Raf (Ser338/Tyr340) AntibodyAnti-Ras Antibody, clone RAS10
Anti-Rsk1 AntibodyAnti-Rsk2/MAPKAP Kinase 1b AntibodyAnti-JNK/SAPK1 AntibodyAnti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) AntibodyAnti-JNK2 AntibodyAnti-JNK3, clone EPR5493(2), Rabbit Monoclonal AntibodyAnti-SHP-2/SHPTP-2 AntibodyAnti-SOS1 Antibody, CTAnti-Src Antibody, clone GD11
Anti-Src Antibody, clone N6L, rabbit monoclonalAnti-phospho-Src Antibody (Tyr215)Anti-phospho-Src (Tyr416) Antibody, clone 9A6Anti-phospho-Src (Tyr418) AntibodyAnti-SRF [Serum Response Factor] Antibody, clone 1E1Anti-TAB1 AntibodyAnti-phospho-TAK1 (Ser412) Antibody
Western Blotting
Western BlottingWestern Blotting
Immunohistochemistry (Paraffin)
Western Blotting;ImmunohistochemistryWestern Blotting
Immunoprecipitation;Western Blotting
Immunocytochemistry;Immunoprecipitation;Western BlottingImmunocytochemistry;Immunoprecipitation;Western Blotting
Immunocytochemistry;Immunoprecipitation;Western BlottingImmunohistochemistry;Immunoprecipitation;Western BlottingImmunoprecipitation;Western BlottingWestern Blotting
ELISA;Flow Cytometry;Immunocytochemistry;Immunohistochemistry;Immunoprecipitation; Western BlottingWestern Blotting;Immunoprecipitation;ELISAImmunoprecipitation;Western Blotting
Immunoprecipitation; Western BlottingWestern Blotting;Enzyme Assay;Immunoprecipitation
ELISA;Western BlottingWestern Blotting;Immunocytochemistry;Flow CytometryWestern Blotting;ImmunocytochemistryImmunoprecipitation; Western BlottingImmunoprecipitation; Western Blotting
Western Blotting
ELISA;Western Blotting
Western Blotting;Immunoprecipitation
Immunocytochemistry;Immunohistochemistry;Western BlottingWestern Blotting;Immunocytochemistry
Western BlottingWestern Blotting
Human;Mouse;Rat
Human;Mouse;RatHuman
Human;Rat;Mouse;Chimpanzee;Rhesus MacaqueHuman;Mouse;RatHuman;Mouse
Human;Mouse;Pig;Rat;XenopusVertebrates
Human;Rat;Mouse;Bovine;Rabbit;Xenopus;Yeast S. cerevisiae
Human;Mouse
Human;Mouse;Rat
Human;Mouse;RatHuman
Human;Rat;Mouse
Human;Mouse;RatHuman;Mouse;Rat
Human;Mouse;RatMouse;Human;Rat;Canine;Xenopus;Chicken;Bovine;HorseHuman;Mouse;RatHuman
Human;MouseHuman;Mouse Avian;Human;Mouse;Mink;Rat
Human
Human
Human;Mouse
Chicken;Human;Mouse
Human;Mouse
Human;Mouse;Rhesus MacaqueHuman;Mouse
100 µL
100 µL100 µL
100 µg
100 µg100 µL
100 µg
200 µL
200 µg
50 µL
250 µg
200 µg100 µL
100 µg
100 µg100 µg
200 µg200 µg
100 µg100 µL
200 µg200 µg200 µg
100 µl
100 µL
100 µg
100 µL
100 µg
100 µg200 µL
29
mTOR Signaling作为对营养和生长因子的反应,PI3激酶/Akt/mTOR通路会被活化并刺激细胞进行增殖和一系列基因的翻译。这个信号通路的失调
会导致多种人类肿瘤。据报道,发现超过30%的实体瘤中包含PI3-K的催化单位发生了突变,致使酶活、细胞增殖、细胞侵袭、
细胞转移等都有所提高。正常情况下,TSC-1和TSC-2形成二聚体复合物,是Rheb的抑制剂,而Rheb是mTOR活化所必需的刺激蛋
白,因此TSC-1/TSC-2在正常情况下抑制mTOR的功能。当Akt活化后,它可磷酸化TSC-2的Ser939和Thr1462,抑制了TSC-1/TSC-2复合
物的形成,从而解除了对Rheb的抑制作用,使得mTOR被激活。活化的mTOR通过磷酸化蛋白翻译过程中的某些因子来参与多项细
胞功能,其中最主要的是4EBP1和P70S6K。
09-345AB606004-1521ABE62105-1527
07-1408
07-1331ABC47709-104
09-230
ABS54407-53207-88607-1586
Anti-Anti-phospho-mTOR (Thr2446) AntibodyAnti-BBIP10 AntibodyAnti-RPA1 p70 Antibody, clone RPA9, 1-30Anti-EIF4A2 AntibodyAnti-eIF4A3 Antibody, clone 3F1
Anti-Glucose Regulating Protein 94 (GRP94) AntibodyAnti-phospho-Rictor (Ser1219) AntibodyAnti-YY1AP1 AntibodyAnti-phospho-Raptor (Ser722) Antibody
Anti-phospho-Raptor (Thr706) Antibody
Anti-phospho-Raptor Antibody (Ser696)Anti-phospho-PKR (Thr446) AntibodyAnti-phospho-PKR (Thr451) AntibodyAnti-HIF-1-alpha Antibody, hydroxyproline (Pro564)
Western BlottingWestern BlottingWestern Blotting;ImmunocytochemistryWestern Blotting;ImmunohistochemistryWestern Blotting;Immunoprecipitation;ImmunocytochemistryWestern Blotting;Immunocytochemistry
Western BlottingWestern Blotting;ImmunohistochemistryDot Blot;Western Blotting
Western Blotting;Dot Blot
Western BlottingWestern BlottingWestern BlottingWestern Blotting;Peptide Inhibition Assay
Human;Mouse;RatHuman;MouseHumanHumanHuman;Mouse
Human;Monkey;Rat
Human;RatHuman;Mouse;RatHuman;Mouse;Rat;Bovine;Canine;Goat;Chicken;HorseHuman;Mouse;Rat;Canine;Bovine;HorseHumanHumanHuman;MouseHuman
100 µL100 µg100 µg150 µL100 µg
100 µL
100 µg100 µg100 µg
100 µg
200 µL100 µg100 µL100 µL
DescriptionCat. No. Key Applications Species Reactivity Qty/Pk
抗体
30
05-123805-151805-1516
05-1214ABS772
MABE102
ABS97ABS625
07-133109-104
09-10709-230
ABS54405-70605-1214MAB126
05-1134
AB5312
AB5220
AB9040
AB1554
AB1526
ABS625
AB5156-50UL
AB5785AB5787AB5795
MAB13170
07-1015
07-1016
07-53207-886AB10181
Anti-PARN Antibody, clone 7A7.1Anti-hnRNP L Antibody, clone 4D11Anti-hnRNP U Antibody, clone 3G6
Anti-FXR2 Antibody, clone 2C8.2Anti-DDX50 Antibody
Anti-FIH-1 Antibody, clone FIH 162c
Anti-KEAP1 AntibodyAnti-PDIA3 Antibody
Anti-phospho-Rictor (Ser1219) AntibodyAnti-phospho-Raptor (Ser722) Antibody
Anti-phospho-Raptor (Ser877) AntibodyAnti-phospho-Raptor (Thr706) Antibody
Anti-phospho-Raptor Antibody (Ser696)Anti-CRMP1 Antibody, clone Y21Anti-FXR2 Antibody, clone 2C8.2Anti-Epidermal Growth Factor Antibody, clone 144-8Anti-Fibroblast Growth Factor Receptor 1Antibody, clone VBS1Anti-Growth Associated Protein 43 Antibody
Anti-Growth Associated Protein-43 (GAP-43) Antibody
Anti-Nerve Growth Factor Antibody, pro
Anti-Nerve Growth Factor ReceptorAntibody, p75Anti-Nerve Growth Factor-β Antibody
Anti-PDIA3 Antibody
Anti-Calcium Channel Antibody, Voltage Gated α 1CAnti-Calcium Channel β 1 AntibodyAnti-Calcium Channel β 2 Antibody, near CTAnti-Calcium Channel γ 4 Antibody
Anti-Cav1.2 calcium channel Antibody,clone L57/46Anti-phospho-Upf1 (Ser1100) Antibody
Anti-phospho-Upf1 (Ser1127) Antibody
Anti-phospho-PKR (Thr446) AntibodyAnti-phospho-PKR (Thr451) AntibodyAnti-Pumilio-2 (PUM2) Antibody
Western Blotting;ImmunocytochemistryWestern Blotting;ImmunoprecipitationWestern Blotting;Immunoprecipitation;Immunocytochemistry;ELISAWestern Blotting;ImmunocytochemistryWestern Blotting;Immunohistochemistry;ImmunocytochemistryWestern Blotting;Immunohistochemistry;ImmunofluorescenceWestern Blotting;ImmunocytochemistryWestern Blotting;Immunohistochemistry;ImmunocytochemistryWestern BlottingDot Blot;Western Blotting
Western BlottingWestern Blotting;Dot Blot
Western BlottingWestern BlottingWestern Blotting;ImmunocytochemistryImmunohistochemistry;Immunoprecipitation
Western Blotting;Immunoprecipitation;Immunohistochemistry (Paraffin);ELISAImmunocytochemistry;Immunohistochemistry;Immunohistochemistry (Paraffin);Immunoprecipitation;Western BlottingELISA;Immunocytochemistry;Immunohistochemistry;Immunoprecipitation;Western BlottingImmunoprecipitation;Western Blotting;ImmunohistochemistryImmunocytochemistry;Immunohistochemistry(Paraffin);ImmunoprecipitationELISA;Western Blotting;Immunohistochemistry;Affects FunctionWestern Blotting;Immunohistochemistry;ImmunocytochemistryImmunohistochemistry;Immunoprecipitation;Western BlottingWestern BlottingWestern BlottingWestern Blotting
Western Blotting;Immunohistochemistry
Western Blotting;Immunoprecipitation
Western Blotting;Immunoprecipitation
Western BlottingWestern BlottingImmunocytochemistry;Immunohistochemistry;Western Blotting
HumanHumanHuman
Human;Rat;MouseHuman
Human
Human;Mouse;RatHuman
Human;RatHuman;Mouse;Rat;Bovine;Canine;Goat;Chicken;HorseHuman;Primate;MonkeyHuman;Mouse;Rat;Canine;Bovine;HorseHumanHumanHuman;Rat;MouseHuman;Mouse
Human;Mouse;Rat;Chicken;Bovine
Bovine;Human;Mouse;Monkey;Rat;Feline
Human;Mouse;Rat;Chicken;Bovine
Human;Mouse;Rat
Human;Mouse;Rat
Chicken;Human;Mouse;Rat
Human
Human;Mouse;Rat;Rabbit
HumanHuman;Mouse;RatHuman;Mouse;Rat
Mouse;Human;Rat;Rabbit
Xenopus;Human;Bovine;Horse;Chicken;Mouse;RatHuman;Xenopus;Bovine;Mouse;Rat;ChickenHumanHuman;MouseHuman;Mouse;Chimpanzee;Rhesus Macaque;Canine
100 µg100 µL100 µg
100 µg100 µL
100 µg
100 µg100 µL
100 µg100 µg
100 µg100 µg
200 µL100 µL100 µg200 µL
100 µg
100 µL
100 µL
100 µL
100 µL
250 µL
100 µL
50 µL
100 µg100 µg100 µg
100 µg
100 µg
100 µg
100 µg100 µL100 µg
DescriptionCat. No. Key Applications Species Reactivity Qty/Pk
抗体
AB5605AB5830MAB3560
AB9890
07-520
MAB5310AB3282
MABN527ABS97MABC71307-685
MABN457
06-984
ABC391AB5380AB5382
AB16301AB553205-233
16-226
16-207
16-310
07-61007-35707-56107-562AB5484MAB5328
ABC245MABC684
AB10346AB5482AB9328
Anti-4-Hydroxynonenal AntibodyAnti-8-Hydroxydeoxyguanosine AntibodyAnti-8-Oxoguanine Antibody, clone 483.15
Anti-AGE (Advanced Glycated End-products) AntibodyAnti-eNOS/NOS III Antibody, CT
Anti-Glutathione Antibody, clone D8Anti-Glutathione-S-Transferase Antibody, S. japonicum formAnti-iNOS/NOS II, clone 13F5.1 AntibodyAnti-KEAP1 AntibodyAnti-KEAP1, clone 7G4B10 AntibodyAnti-Lactotransferrin Antibody
Anti-Mitochondrial dicarboxylate carrier Antibody, clone 1F5.1Anti-Mn-SOD Antibody
Anti-NDUFB7 AntibodyAnti-Nitric Oxide Synthase I AntibodyAnti-Nitric Oxide Synthase II Antibody
Anti-Nitric Oxide Synthase III AntibodyAnti-Nitrotyrosine AntibodyAnti-Nitrotyrosine Antibody, clone 1A6
Anti-Nitrotyrosine Antibody, clone 1A6, Alexa Fluor® 488 conjugateAnti-Nitrotyrosine Antibody, clone 1A6, HRP conjugateAnti-Nitrotyrosine Magnetic Bead Conjugate AntibodyAnti-Peroxiredoxin 2 AntibodyAnti-phospho-eNOS/NOS III (Ser116) AntibodyAnti-phospho-eNOS/NOS III (Ser617) AntibodyAnti-phospho-eNOS/NOS III (Ser635) AntibodyAnti-RAGE AntibodyAnti-RAGE Antibody, clone DD/A11 or A11
Anti-REDD1 AntibodyAnti-SOD1 Antibody, clone 6F5
Anti-SOD2 AntibodyAnti-Superoxide Dismutase 1 AntibodyAnti-Thioredoxin 1 Antibody
ELISA;Western BlottingELISA;Immunohistochemistry (Paraffin)ELISA;Immunocytochemistry;Chromatin Immunoprecipitation (ChIP)ELISA;Western Blotting
Western Blotting
Western BlottingELISA;Western Blotting
Western Blotting;ImmunohistochemistryWestern Blotting;ImmunocytochemistryImmunohistochemistry;Flow Cytometry;ELISAImmunoprecipitation;Western Blotting;Immunocytochemistry;Immunohistochemistry;Immunohistochemistry (Paraffin)Western Blotting;Immunohistochemistry
Immunohistochemistry;Immunohistochemistry (Paraffin);Western BlottingWestern Blotting;ImmunohistochemistryImmunohistochemistry;Western BlottingImmunocytochemistry;Immunohistochemistry;Western BlottingWestern Blotting;ImmunocytochemistryELISA;ImmunohistochemistryImmunocytochemistry;Immunohistochemistry;Immunoprecipitation;Western BlottingFlow Cytometry;Western Blotting
ELISA;Western Blotting
Immunoprecipitation
Western Blotting;ImmunocytochemistryWestern BlottingWestern BlottingWestern BlottingELISA;Immunohistochemistry (Paraffin)ELISA;Immunocytochemistry;Immunohistochemistry;Western BlottingWestern Blotting;Immunohistochemistry (Paraffin)Western Blotting;Immunocytochemistry;Flow CytometryWestern BlottingWestern BlottingWestern Blotting
HumanHumanHuman;Rat;Mouse;Monkey
Human
Human;Rat;Bovine;Mouse;Canine;Guinea Pig;PigEukaryoteVertebrates;Schistosoma japonicum
HumanHuman;Mouse;RatHumanHuman;Mouse;Rat;Hamster;Monkey
Mouse;Rat
Human;Rat;Mouse;Bovine
Human;MouseHuman;Mouse;Monkey;Rat;SheepMouse;Rat
HumanAllVertebrates
Vertebrates
All
All
HumanBovine;HumanBovineBovineHumanBovine;Human;Mouse
Human;Mouse;RatHuman;Mouse
Human;Rat;MouseMouse;RatMouse;Rat
200 µL200 µL100 µL
200 µL
200 µg
100 µg500 µg
100 µL100 µg100 µg100 µL
100 µg
200 µg
150 µL50 µL50 µL
100 µg100 µL100 µg
100 µg
50 µL
200 µL
50 µL100 µL200 µL200 µL200 µL100 µg
100 µg100 µL
50 µL50 µL100 µL
31
Milliplex kit产品描述 货号
抑制剂
124018-1MGCN124033-10MGCN171260-1MGCN203695-10MGCN234501-5MGCN371963-1MGCN475988-5MGCN475990-5MGCN521276-5MGCN528100-1MGCN528113-1MGCN529584-5MGCN553210-1MGCN559273-10MGCN565835-10MGCN565840-10MGCN662005-1MGCN
Akt Inhibitor VIII, Isozyme-SelectiveAkt Inhibitor XVI, AT7867AMPK Inhibitor, Compound CbpV(phen)Compound 401H-89, DiHClmTOR Inhibitor III, PP242mTOR Inhibitor IV, Ku-63794PDK1 Inhibitor IIPI-103PI 3-Kbeta Inhibitor VI, TGX-221PTK/PI 3-K/mTOR Inhibitor, PP121RapamycinS6K1 Inhibitor, PF-4708671SHIP1 Inhibitor, 3ACSHIP2 Inhibitor, AS1938909U0126 1,4-DIAMINO-2,3-DICYANO-1,4-BIS...
Akt/mTOR(Phosphoprotein)-11Plex(Cat.No.48-611MAG)AB2
Analyte Total Phosphorylated
Akt/PKB
GSK3α
GSK3β
IGF1R
IR
IRS1
mTOR
p7OS6 Kinase
PTEN
RPS6
TSC2
(Ser473)
(Ser21)
(Ser9)
(Tyr1135/1136)
(Tyr1162/1163)
(Ser636)
(Ser2448)
(Thr389/412)
(Ser380)
(Ser235/236)
(Ser939)
H,M,R
H,M,R
H,M,R
H,M
H
H,R
H,M
H,M,R
H,M,R
H,M,R
H,M,R
Akt/mTOR(Total)-11Plex(Cat.No.48-612MAG)AB2
Analyte Total Phosphorylated
Akt/PKB
GSK3α
GSK3β
IGF1R
IR
IRS1
mTOR
p7OS6 Kinase
PTEN
RPS6
TSC2
H,M,R
H,M,R
H,M,R
H,M,R
H,R
H,M,R
H,M,R
H,M,R
H,M,R
H,M,R
H,M,R
mTOR Phospho/Total -2 Plex(Cat.No.48-625MAG)AB2
Analyte Total Phosphorylated
mTOR
mTOR
H,M,R
H,M
(Ser2448)
32
Hayden, M.S., and Ghosh, S. 2012. Genes Dev. 26, 203.Ben-Neriah, Y., and Karin, M. 2011. Nat. Immunol. 12, 715.Oeckinghaus. A., et al. 2011. Nat. Immunol. 8, 695Karin, M., and Greten, F.R. 2005. Nat. Rev. Immunol. 5, 749
参考文献Bouwmeester, T., et al. 2004. Nat. Cell. Biol. 6, 97.Chen, L.F., and Greene, W.C. 2004. Nat. Rev. Mol. Cell Biol. 5, 392。Karin. M., et al. 2004. Nat. Rev. Drug Disc. 3, 17
NF-κB SignalingNF-κB在细胞因子诱导的基因表达中起关键性的调控作用,它调控的基因编码急性期反应蛋白、细胞因子、细胞粘附分子、免疫调节分子、病毒瘤基因、生长因子、转录和生长调控因子等。通过调控多种基因的表达,NF-κB参与免疫反应、炎症反应、细胞凋亡、肿瘤发生等多种生物进程。
NF-κB主要有两种活化方式:一、经典的NF-κB活化途径:静止状态时,NF-κB以无活性的潜在状态存在于细胞浆中,它与抑制因子IkB结合组成一个三聚体p50-p65-IkB;在IkBs激酶(IKK)催化IkBs的两个保守的丝氨酸残基磷酸化;IkBs在SCF-E3泛素化酶复合体的催化作用下多泛素化而被蛋白酶降解;活化的NF-κB转位到核内同与其相关的DNA基序结合以诱导靶基因转录;活化的NF-κB快速诱导编码自身抑制剂IkBa的基因的转录;新合成的IkBa进入细胞核,使NF-κB与DNA解离并排出细胞核,等待重新激活。二、非经典的NF-κB活化途径:广泛的IkBs家族也包括P50和P52前体形式的NF-κB1和NF-κB2,分别是P105和P100。除了P50和P52序列外,这些前体还包括IkB样的锚蛋白区,它抑制与其相关的NF-κB亚单位的活性。从前体产生P50和P52的过程还没有被人完全的理解,但它需要翻译时和翻译后的蛋白酶加工处理过程。在翻译的同时就会组成性的产生约等量的P50和P105,虽然这时P50还没有加工完成。P52的产生主要但不完全是由于信号诱导的P100加工完成的。不像是IkBa、IkBβ、IkBε的降解,信号诱导的磷酸化及加工P100成P52不需要经典的IKK-γ依赖的信号途径。IKK-a和NF-κB诱导激酶(NIK)是必不可少的,但IKK-β和IKK-γ是不需要的。
Phosphorylation
Ubiquitin
Calbiochem® Inhibitors
Nucleus
Bacterial Antigens
Growth Factors
Growth FactorReceptors
TNFα
Proteosome
TNFR1 IL-1R
IL-1
• PDK/Akt/Flt Dual
PathwayInhibitor
• Interluekin-1 Receptor-AssociatedKinase-1/4 Inhibitor
• PD 98059
• (5Z)-7-Oxozeaenol, Curvularia sp.
• IKK Inhibitor VII
• IKK Inhibitor VII
• NEMO-Binding Domain BindingPeptide, Cell Permeable • BAY 11-7082
• Tpl2 Kinase Inhibitor
• Histone Acetyltransferase Inhibitor II• Trichostatin A,Streptomyces sp.
• JNK Inhibitor II
• MG-132
• BAY 11-7082
p52RelB
NF-κB
p52p54
NF-κBNF-κB
p50c-Rel
NF-κB
p50c-Rel
NF-κB
IκBs
HDAC3
p50
p50
p50Bcl3
p50
p105p105
ABIN-2Tpl2
LPS
TRADDTRAF2
p300
CBP
TRAF6
TRAF6
MALPPGN
MyD88TOLLIPMyD88
PI 3-K
PDK-1
ASK1
MKK4,7
JNKsAkt/PKB
IKK-γ
MEKKs
Tpl2
MEKK
ERK
PKR PKR
IRAKIRAK
IRAK
TAK1
IKK-α IKK-β
JNK PathwayERK
Signaling
Tpl2Degradation
Virus
IκB Degradation
Nuclear Import
SCF/β-TrCP
IκBs
RelBp52NF-κB
IκBs
IκBs
p50c-Rel
NF-κB
IκBs p52
p65
NF-κB
κ-BRas
IκBs p52
p65
NF-κB
IκBs p52
p65
NF-κB
PACT
Nuclear Export
AcetylationDeacetylation
33
Description Qty/Pk Catalogue No.
BAY 11-7082 1 mg 196870
IKK Inhibitor VII 1 mg 401486
Interleukin 1- Receptor-Associated-Kinase-1/4 Inhibitor 5 mg 407601
Histone Acetyltransferase Inhibitor II 10 mg 382110
MG-132 5 mg 474790
NEMO-Binding Domain-Binding Peptide, Cell-Permeable 500 µg 480025
NF-κB SN50, Cell-Permeable Inhibitor Peptide 500 µg 481480
PD 98059 5 mg 513000
PDK1/Akt/Flt Dual Pathway Inhibitor 5 mg 521275
Trichostatin A, Streptomyces sp. 1 mg 647925
TIRAP Inhibitor Peptide, Cell-Permeable 1 mg 613570
Tpl2 Kinase Inhibitor 1 mg 616373
(5Z)-7-Oxozeaenol, Curvularia sp. 1 mg 499610
JNK inhibitor II 5 mg 420119
Anhydrous DMSO 15 mL
InhibitorSelect™ NF-κB Signaling Pathway Inhibitor Panel (Catalogue No. 481487)
The Inhibitor panel includes:
NF-κB抗体Description Species Applications Qty Catalogue No.
Anti-IκB-α Antibody Human, Mouse IB, IP 200 µg 06-494
Anti-IκBα Human, Mouse, Rat ELISA, IB, IP, (IH)P 100 µL 07-1483
Human, Monkey, Mouse IB 100 µg 05-536
Anti-IKKα Mouse mAb (14A231) Human, Monkey, Mouse IB 100 µg OP133
Anti-IKKα Most mammalian species IB, IP 150 µg 07-1007
Anti-IKKα, clone Y463, Rabbit Monoclonal Human, Mouse, Rat IB, IC, IP, FC, IH(P) 100 µL 04-365
Anti-IKKβ, Mouse Monoclonal (10AG2) Human IB 100 µg OP134
Anti-IKKβ, Mouse Monoclonal (10AG2) Human IB 100 µg OP134
Anti-IKKβ, clone 10AG2 Human, Mouse, Rat IB 100 µg 05-535
Anti-IKKβ Human, Mouse IB, IH(P) 100 µg 07-1479
Anti-IKKβ Most mammalian species IB 150 µg 07-1008
Anti-IKKβ, clone Y466, Rabbit Monoclonal Human, Mouse FC, IB, IH(P), IP 100 µL 04-366
Anti-IKKγ Human, Mouse IB 100 µg 05-631
Anti-IKKε Antibody Human, Mouse IB 200 µg 07-580
Anti-phospho-IKKε (Ser172) Human, Mouse, Rat IB 100 µL 06-1340
Anti- NF-κB p50, clone E381 Human, Mouse, Rat FC, IB, IC, IH, IH(P) 100 µL 04-234
Anti- NF-κB p50 Human, Mouse IB, IP, ChiP 150 µL 06-886
Anti-NF-κB p52 Human IB, IP 100 µL 06-413
Anti-NF-κB p65 Human, Mouse, Rat IB, IP 100 µL ABE136
Anti-NF-κB (p65, RelA) (Ab-2) Rabbit Human ELISA, EMSA, IB, IP 100 µL PC138
Anti-NF-κB, p65 Subunit, Active Subunit, clone 12H11
Human, Mouse, Rabbit, Rat EMSA, FC, IB, IC, IH(P) 100 µg MAB3026
Anti-NF-κB p52 Human EMSA, IB, IP 200 µg 05-361
Anti-NF-κB p65 Human, Mouse, Rat IB, IP 100 µg ABE347
Anti-NF-κB p65, C-Terminus Human, Mouse, Rat EMSA, IB, IP 100 µg 06-418
Anti-NF-κB, p65, clone 1G10.2 Bovine, Human, Mouse, Rat IB, IC, IF 200 µg 05-1469
Anti-NF-κB p65, Phospho-speci�c (Ser276) Human ELISA, IB 100 µL AB3375
Anti-NF-κB p65, clone EP2161Y Human FC, IB, IC 100 µL 04-1008
Anti-Phospho-NF-κB p65 (Ser536), clone EP2294Y
Human IB, IP 100 µL 04-1000
参考文献:Recruitment of IκBα to the hes1 promoter is associated with transcriptional repression. PNAS. doi: 10.1073/pnas.0404429101
COMMD proteins, a novel family of structural and functional homologs of MURR1. JBC. doi: 10.1074/jbc.M501928200
参考文献:Nonredundant and complementary functions of TRAF2 and TRAF3 in a ubiquitination cascade that activates NIK-dependent alternative NF-B signaling.Nature Immunology 9, 1364 - 1370 ,2008
zVAD-induced necroptosis in L929 cells depends on autocrine production of TNFα mediated by the PKC–MAPKs–AP-1 pathway.Cell Death and Differentiation (2011) 18, 26–37
The synthetic epoxyquinoids jesterone dimer and epoxyquinone A monomer induce apoptosis and inhibit REL (human c-Rel) DNA binding in an IκBα-de�cient diffuse large B-cell lymphoma cell line.Cancer Letters. doi:10.1016/j.canlet.2005.10.004
参考文献:Anaplasma phagocytophilum dihydrolipoamide dehydrogenase 1 affects host-derived immunopathology during microbial colonization. Chen, Gang, et al.Infect. Immun., 80: 3194-205,2012
Human herpes simplex viruses in benign and malignant thyroid tumours. Jensen K, Patel A, Larin A, Hoperia V, Saji M, Bauer A, Yim K, Hemming V, Vasko V. J Pathol 221 193-200. 2010
参考文献:Nuclear factor-kappaB induced by doxorubicin is de�cient in phosphorylation and acetylation and represses nuclear factor-kappaB-dependent transcription in cancer cells. Wai Chi Ho, Kathleen M Dickson, Philip A Barker. Cancer research,65:4273-81, 2005
Ineffectiveness of histone deacetylase inhibitors to induce apoptosis involves the transcriptional activation of NF-kappa B through the Akt pathway. Mayo, M. W., et al J Biol Chem, 278:18980-9, 2003
*以上组分可按目录号单独购买
34
AB9730
AB9746
AB9732
MABC749
ABE1007AB10410
AB1579407-53507-822
05-797R
05-1152
07-507
MABE329
MABS1190
06-225
MABS15006-47806-659
06-828
05-107607-1333
05-1072
05-1028
MABS64
ABS29
09-272
ABS29
05-747
07-64107-852
07-641
Anti-14-3-3 β Antibody
Anti-14-3-3 ζ Antibody
Anti-14-3-3 ε Antibody, CT
Anti-phospho-BAD (Ser112), clone EPR1891(2), Rabbit Monoclonal AntibodyAnti-BATF3 AntibodyAnti-YES (Proto-oncogene c-Yes) AntibodyAnti-Dexras1 AntibodyAnti-MKP1 AntibodyAnti-phospho-eIF2Bε (Ser539) AntibodyAnti-phospho-Erk1/2 (Thr202/Tyr204, Thr185/Tyr187)Antibody, recombinant clone AW39R, rabbit monoclonalAnti-Erk1/2 (p44/p42) Antibody, clone MK12
Anti-phospho-BMK1/Erk5 (Thr218/Tyr220) AntibodyAnti-c-Fos Antibody, clone 2G9C3Anti-Glucokinase/GCK Antibody, clone 9G1.2 Anti-c-Jun Antibody
Anti-HGK Antibody, clone 2H1.1Anti-HSP27 AntibodyAnti-phospho-c-Jun (Ser73) AntibodyAnti-phospho-c-Jun (Ser63) AntibodyAnti-c-Jun Antibody, clone 6E4.4Anti-JunB Antibody
Anti-Ras Antibody, (K-, H-, N-), clone 9A11.2Anti-phospho-MAPKAPK2 (Thr334) Antibody, clone 9F10.2Anti-phospho-p38 (Thr180/Tyr182) Antibody, clone 6E5.2Anti-p38/SAPK2A Antibody
Anti-phospho-p38α (Thr180/Tyr182) AntibodyAnti-p38/SAPK2A Antibody
Anti-phospho-MEK1 (Ser218/222)/MEK2 (Ser222/226) Antibody, rabbit monoclonalAnti-MEK1 AntibodyAnti-phospho-MEK1 (Thr292) AntibodyAnti-MEK1 Antibody
ELISA;Western Blotting;Immunohistochemistry
ELISA;Western Blotting;Immunohistochemistry
ELISA;Western Blotting;Immunohistochemistry
Western Blotting
Western Blotting;Flow CytometryWestern Blotting
Western BlottingWestern BlottingWestern Blotting
Multiplexing;Western Blotting
Western Blotting;Immunoprecipitation;Immunohistochemistry (Paraffin);ImmunocytochemistryWestern Blotting;Peptide Inhibition Assay
Western Blotting;Immunocytochemistry
Western Blotting;Immunohistochemistry (Paraffin)Immunoprecipitation;Western Blotting;ImmunocytochemistryWestern BlottingWestern Blotting;ImmunohistochemistryImmunocytochemistry;Immunoprecipitation;Western BlottingWestern Blotting
Western Blotting;ImmunocytochemistryImmunocytochemistry;Western Blotting
Western Blotting;Immunohistochemistry (Paraffin)Immunofluorescence;ELISA;Western Blotting
Western Blotting;Immunocytochemistry
Western Blotting
Immunoprecipitation; Western Blotting
Western Blotting
Western Blotting
Immunoprecipitation; Western BlottingWestern Blotting
Immunoprecipitation; Western Blotting
Bovine;Human;Mouse;Rat;Rabbit;SheepBovine;Chicken;Human;Mouse;Rat;Rabbit;SheepBovine;Chicken;Human;Mouse;Rat;Rabbit;SheepHuman
HumanHuman;Horse
MouseHuman;MouseHuman;Rat
Human;Mouse;Rat
Human;Mouse;Rat;Canine;Xenopus;Bovine;Chicken
Human;Mouse;Rat;Bovine
Human
Human
Avian;Human;Mouse
RatHumanHuman;Mouse
Human;Mouse;Rat
Human;Mouse;RatHuman;Mouse;Rat;Canine;Horse;BovineHuman;Mouse;Rat
Human;Mouse;Rat
Human;Mouse
Human;Mouse;Rat;Horse;Rhesus MacaqueHuman;Mouse;Rat
Human;Mouse;Rat;Horse;Rhesus MacaqueHuman;Mouse;Rat
Human;Mouse;RatHuman;Mouse
Human;Mouse;Rat
100 µL
100 µL
100 µL
100 µL
100 µg100 µg
100 µL100 µg100 µL
100 µL
100 µg
200 µL
100 µg
100 µg
200 µL
100 µg250 µg100 µL
100 µL
100 µg100 µg
100 µg
100 µg
100 µg
100 µg
100 µL
100 µg
100 µL
200 µg100 µL
200 µg
Applications:ChIP: Chromatin ImmunoprecipitationDB: Dot BlottingEMSA: Electrophoretic Mobility Supershift Assay ELISA: Enzyme-linked Immunosorbent Assay FC: Flow Cytometry IB: Immunoblotting IC: Immunocytochemistry IH: ImmunohistochemistryIP: ImmunoprecipitationPIA: Peptide Inhibition Assay
Description Species Applications Qty Catalogue No.
Anti-NF-κB p65, C-Terminus Human, Mouse, Rat EMSA, IB, IP 100 µg 06-418
Anti-NF-κB, p65, clone 1G10.2 Bovine, Human, Mouse, Rat IB, IC, IF 200 µg 05-1469
Anti-NF-κB p65, Phospho-speci�c (Ser276) Human ELISA, IB 100 µL AB3375
Anti-NF-κB p65, clone EP2161Y Human FC, IB, IC 100 µL 04-1008
Anti-Phospho-NF-κB p65 (Ser536), clone EP2294Y
Human IB, IP 100 µL 04-1000
Anti-Phospho-NF-κB (p100) (Ser707) Human DB, IB, PIA 200 µL 07-1829
Anti-NIK, C-terminus Human IB 100 µg AB16528
Anti-RelB Human IB, IC, IF 200 µL 07-1332
Anti-RelB Human, Mouse, Rat, Rhesus Monkey
IB, IH(P) 100 µg 06-1105
Anti-c-Rel (NF-κB) Human ELISA, EMSA, IB 100 µg 09-040
Anti-TBK1, clone AOW9 Canine, Chimpanzee, Human, Mouse
IB, IP 100 µL 04-856
Anti-TBK1/NAK (N-term), clone EP611Y Human, Mouse, Rat ICC, IB, IH(P),IP 100 µL 04-387
Anti-TBK1 Mouse (108A429) Human IB 100 µg AM76
Anti-Phospho-TBK1 (Ser172) Human, Mouse, Rat IB 100 µL 07-2192
参考文献:Nuclear factor-kappaB induced by doxorubicin is de�cient in phosphorylation and acetylation and represses nuclear factor-kappaB-dependent transcription in cancer cells. Wai Chi Ho, Kathleen M Dickson, Philip A Barker. Cancer research,65:4273-81, 2005
Ineffectiveness of histone deacetylase inhibitors to induce apoptosis involves the transcriptional activation of NF-kappa B through the Akt pathway. Mayo, M. W., et al J Biol Chem, 278:18980-9, 2003
NF-κB抑制剂Description Comments Qty/Pk Catalogue No.
Andrographolide An irreversible blocker of NF-κB and AP-1 (IC50 ≤ 15 µM) activation with anti-in�ammatory, anti-apoptotic properties.
50 mg 172060
Aurothiomalate Blocks NF-κB activation and PKCι-dependent Rac1 signaling. Modi�es metal sensitive cysteine residues of IKK and PKCι, and inhibits the activity of IKK (IC50 = 10.9 µM).
50 mg 189401
Bay 11-7082 Selective, irreversible inhibitor of TNFα-inducible phosphorylation of IκB-α (IC50 = 10 µM) without affecting the constitutive IκB-α phosphorylation. Reduces the nuclear translocation of NF-κB.
10 mg 196870
InSolution™ BAY 11-7082 A 100 mM (10 mg/483 µL) solution of BAY 11-7082 (Catalogue No. 196870) in DMSO.
10 mg 196871
Bay 11-7085 Exhibits biological properties similar to that of BAY 11-7082 (Catalogue No. 196870). BAY 11-7085 has also been shown to have potent anti-in�ammatory properties in vivo.
10 mg 196872
CAPE (Caffeic Acid Phenethyl Ester, Synthetic) A cell-permeable active component of propolis from honeybee hives with antiviral, anti-in�ammatory, and immunomodulatory properties. Has been shown to act as a potent and speci�c inhibitor of NF-κB activation.
25 mg 211200
(E)-Capsaicin An active constituent of cayenne pepper with excitatory and desensitizing effects on a subset of primary afferent sensory neurons. Inhibits NF-κB activation by TNF.
100 mg 211275
EF24 (3,5-bis-(2-Fluorobenzylidene)-piperidin-4-one)
A cell-permeable analog of Curcumin that acts as a potent inhibitor of IKKβ inhibitor (IC50 = 1.9 µM) and is shown to block and NF-κB nuclear translocation (IC50 = 1.3 µM).
10 mg 324510
Gliotoxin, Gladiocladium �mbriatum An immunosuppressive secondary metabolite produced by several pathogenic fungi. Speci�cally inhibits NF-κB in B and T cells in nanomolar concentrations.
1 mg 371715
Hypoestoxide, Hypoestes rosea A naturally occurring, cell-permeable diterpene that acts as a selective and direct inhibitor of IκB kinase (IC50 = 24 µM) in TNF-α stimulated HeLa cells thereby prevents NF-κB activation.
1 mg 401006
IκB Kinase Inactive Control Peptide, Cell-Permeable (Ac-AVALLPAVLLALLAPDDRHDAGLDAMKDE-NH2)
An inactive control for IκB Kinase Inhibitor Peptide (Catalogue No. 401477). A 14-amino acid peptide corresponding to the mutated recognition sequence of IκB (Ser32 → Ala and Ser36 → Ala), linked to the hydrophobic region of the �broblast growth factor signal peptide.
1 mg 401478
IκB Kinase Inhibitor Peptide, Cell-Permeable (Ac-AAVALLPAVLLALLAPDDRHDSGLDSMKDE-NH2)
A 14-amino acid peptide corresponding to the active IκB phosphorylation recognition sequence, linked to the hydrophobic region of the �broblast growth factor signal peptide to aid in cellular delivery. Speci�cally inhibits LPS-induced IκB degradation by IκB kinases (IKK) in RAW 264.7 cells (<50 µg/mL) and prevents NF-κB activation.
1 mg 401477
35
07-853
07-69136-010
ABS94
ABC3607-865
07-250
05-577
05-421
07-604
05-389
07-45307-814
05-516
07-147706-918
06-74807-175
05-986MABS771
06-11807-33705-184
04-889
07-791
05-677
07-909
MAB4369
06-133606-1425
Anti-phospho-MEK1 (Thr386) AntibodyAnti-MEKK3 AntibodyAnti-phospho-MKK7/SKK4 (Thr275/Ser277) AntibodyAnti-MLK1 Antibody
Anti-PEA-15 AntibodyAnti-phospho-PED/PEA-15 (Ser116) AntibodyAnti-PP2A Antibody, A subunit
Anti-PP2A Antibody, C subunit, demethylated, clone 4b7Anti-PP2A Antibody, C subunit, clone 1D6
Anti-Rac2 Antibody
Anti-Rac1 Antibody, clone 23A8
Anti-B-Raf AntibodyAnti-phospho-c-Raf (Ser338/Tyr340) AntibodyAnti-Ras Antibody, clone RAS10
Anti-Rsk1 AntibodyAnti-Rsk2/MAPKAP Kinase 1b AntibodyAnti-JNK/SAPK1 AntibodyAnti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) AntibodyAnti-JNK2 AntibodyAnti-JNK3, clone EPR5493(2), Rabbit Monoclonal AntibodyAnti-SHP-2/SHPTP-2 AntibodyAnti-SOS1 Antibody, CTAnti-Src Antibody, clone GD11
Anti-Src Antibody, clone N6L, rabbit monoclonalAnti-phospho-Src Antibody (Tyr215)Anti-phospho-Src (Tyr416) Antibody, clone 9A6Anti-phospho-Src (Tyr418) AntibodyAnti-SRF [Serum Response Factor] Antibody, clone 1E1Anti-TAB1 AntibodyAnti-phospho-TAK1 (Ser412) Antibody
Western Blotting
Western BlottingWestern Blotting
Immunohistochemistry (Paraffin)
Western Blotting;ImmunohistochemistryWestern Blotting
Immunoprecipitation;Western Blotting
Immunocytochemistry;Immunoprecipitation;Western BlottingImmunocytochemistry;Immunoprecipitation;Western Blotting
Immunocytochemistry;Immunoprecipitation;Western BlottingImmunohistochemistry;Immunoprecipitation;Western BlottingImmunoprecipitation;Western BlottingWestern Blotting
ELISA;Flow Cytometry;Immunocytochemistry;Immunohistochemistry;Immunoprecipitation; Western BlottingWestern Blotting;Immunoprecipitation;ELISAImmunoprecipitation;Western Blotting
Immunoprecipitation; Western BlottingWestern Blotting;Enzyme Assay;Immunoprecipitation
ELISA;Western BlottingWestern Blotting;Immunocytochemistry;Flow CytometryWestern Blotting;ImmunocytochemistryImmunoprecipitation; Western BlottingImmunoprecipitation; Western Blotting
Western Blotting
ELISA;Western Blotting
Western Blotting;Immunoprecipitation
Immunocytochemistry;Immunohistochemistry;Western BlottingWestern Blotting;Immunocytochemistry
Western BlottingWestern Blotting
Human;Mouse;Rat
Human;Mouse;RatHuman
Human;Rat;Mouse;Chimpanzee;Rhesus MacaqueHuman;Mouse;RatHuman;Mouse
Human;Mouse;Pig;Rat;XenopusVertebrates
Human;Rat;Mouse;Bovine;Rabbit;Xenopus;Yeast (<italic>S. cerevisiae</italic>)Human;Mouse
Human;Mouse;Rat
Human;Mouse;RatHuman
Human;Rat;Mouse
Human;Mouse;RatHuman;Mouse;Rat
Human;Mouse;RatMouse;Human;Rat;Canine;Xenopus;Chicken;Bovine;HorseHuman;Mouse;RatHuman
Human;MouseHuman;MouseAvian;Human;Mouse;Mink;RatHuman
Human
Human;Mouse
Chicken;Human;Mouse
Human;Mouse
Human;Mouse;Rhesus MacaqueHuman;Mouse
100 µL
100 µL100 µL
100 µg
100 µg100 µL
100 µg
200 µL
200 µg
50 µL
250 µg
200 µg100 µL
100 µg
100 µg100 µg
200 µg200 µg
100 µg100 µL
200 µg200 µg200 µg
100 µl
100 µL
100 µg
100 µL
100 µg
100 µg200 µL
Description Comments Qty/Pk Catalogue No.
IκB Kinase Inhibitor Peptide, Cell-Permeable (Ac-AAVALLPAVLLALLAPDDRHDSGLDSMKDE-NH2)
A 14-amino acid peptide corresponding to the active IκB phosphorylation recognition sequence, linked to the hydrophobic region of the �broblast growth factor signal peptide to aid in cellular delivery. Speci�cally inhibits LPS-induced IκB degradation by IκB kinases (IKK) in RAW 264.7 cells (<50 µg/mL) and prevents NF-κB activation.
1 mg 401477
IKK Inhibitor II, Wedelolactone A cell-permeable, selective, and irreversible inhibitor of IKKα and IKKβ (IC50 < 10 µM). Inhibits NF-κB -mediated gene transcription in cells by blocking the phosphorylation and degradation of IκBα.
1 mg 401474
IKK Inhibitor III, BMS-345541 (4-(2’-Aminoethyl)amino-1,8-dimethylimidazo[1,2-a]
quinoxaline)
A cell-permeable, potent, selective, and allosteric site-binding inhibitor of IKK-2 (IC50 ~300 nM) that displays anti-in�ammatory properties. Exhibits ~10-fold greater selectivity for IKK-2 over IKK-1 (IC50 ~4.0 µM).
1 mg 401480
IKK Inhibitor VII A cell-permeable benzamido-pyrimidine compound that acts as a potent, selective, and ATP-competitive inhibitor of IKK (IC50 = 40 nM, 70 nM, and 200 nM for IKK-2, IKK complex, and IKK-1, respectively).
1 mg 401486
IKK Inhibitor X (N-(6-Chloro-9H-β-carbolin-8-yl)nicotinamide)
A cell-permeable β-carboline that acts as a potent, ATP-competitive, and reversible inhibitor of IKK (IC50 = 88 nM) with selectivity over 14 other commonly studied kinases (IC50 > 100 µM), including NF-κB inducing kinase.
5 mg 401489
IKK Inhibitor XII A cell-permeable amino-diarylbenzamide compound that acts as a potent ATP site-targeting inhibitor against IKK-1 and IKK-2 (pIC50 = 6.4 and 7.0, respectively).
5 mg 401491
IKK Inhibitor XIII, 17-AJB A cell-permeable, irreversible inhibitor against IKK-β activity (IC50 = 300 nM). 1 mg 401493
IKK-2 Inhibitor IV ([5-(p-Fluorophenyl)-2-ureido]thiophene-3-carboxamide,
TPCA-1)
A cell-permeable, reversible, ATP-competitive, and potent inhibitor of IKK-2 (IC50 = 18 nM) that exhibits selectivity over IKK-1, JNK, and p38 MAPK.
500 µg 401481
InSolution™ IKK-2 Inhibitor IV A 10 mM (500 µg/179 µL) solution of IKK-2 Inhibitor IV (Catalogue No. 401481) in DMSO.
500 µg 401484
IKK-2 Inhibitor V (N-(3,5-Bis-tri�uoromethylphenyl)-5-chloro-2-
hydroxybenzamide, IMD-0354)
A cell-permeable IKK-2 inhibitor that selectively blocks IκBα phosphorylation (IC50 ~250 nM) and prevents the induction of NF-κB p65 nuclear translocation.
5 mg 401482
IKK-2 Inhibitor VI (5-Phenyl-2-ureido)thiophene-3-carboxamide)
An ureido-thiophenecarboxamide compound that acts as a potent, cell-permeable, reversible, and ATP-competitive inhibitor of IKK-2 (IC50 = 13 nM).
1 mg 401483
IKK-2 Inhibitor VIII (2-Amino-6-(2-(cyclopropylmethoxy)-6-hydroxyphenyl)-4-(4-
piperidinyl)-3-pyridinecarbonitrile, ACHP)
A cell-permeable piperidinyl-pyridine compound that acts as a selective inhibitor of IKK-2 (IC50 = 8.5 and 250 nM for IKK-2 and IKK-1, respectively).
1 mg 401487
IKK-2 Inhibitor XI (5-Phenyl-3-ureido)-thiophene-2-carboxamide
A cell-permeable thiophenecarboxamide-ureido compound that acts as a potent, ATP-binding pocket-targeting inhibitor of IKK-2 (IC50 = 25 nM).
5 mg 401490
IKK-2 Inhibitor, SC-514 A cell-permeable, potent, reversible, ATP-competitive and highly selective inhibitor of IKK-2 (IC50 ~3-12 µM for IKK-2 homodimer, IKK-1/IKK-2 heterodimer, and IKK-2).
1 mg 401479
InSolution™ IKK-2 Inhibitor, SC-514 A 25 mM (1 mg/178 µL) solution of IKK-2 Inhibitor, SC-514 (Catalogue No. 401479) in DMSO.
1 mg 401485
IKK-3 Inhibitor IX (5-(5,6-Dimethoxybenzimidazol-1-yl)-3-(2-methanesulfonyl-
benzyloxy)-thiophene-2-carbonitrile
A thiophenecarbonitrile compound that acts as a potent ATP-competitive inhibitor of IKK-3 (IKK-ε, IC50 = 40 nM).
5 mg 401488
Isohelenin, Inula sp. A cell-permeable sesquiterpene lactone that acts as a highly speci�c, potent, and irreversible inhibitor of NF-κB activation by preventing IκBα degradation. Does not affect the DNA binding activity of activated NF-κB.
1 mg 416157
NEMO-Binding Domain Binding Peptide, Cell-Permeable (DRQIKIWFQNRRMKWKKTALDWSWLQTE)
A cell-permeable Antennapedia-NBD (wild type) fusion peptide that blocks the association of NEMO with the IKK complex and prevents NF-κB activation.
500 µg 480025
NBD-Binding Peptide II, Cell Permeable, CTP-NBD (CTP; YGRRARRRARR; NBD; TALDWSWLQTE)
A cell-permeable cytoplasmic transduction peptide (CTP) fused to the NEMO binding domain (NBD). Inhibits TNFα-stimulated NF-κB activity (IC50 ~ 50 µM in HCT116 cells). Interferes with the formation of active IKK complex and thereby prevents IκBα phosphorylation and its subsequent proteasomal degradation.
5 mg 480026
NEMO-Binding Domain Binding Peptide, Cell-Permeable, Negative Control (DRQIKIWFQNRRMKWKK-TALDASALQTE)
A cell-permeable, Antennapedia-NBD mutated (Trp739 → Ala and Trp741 → Ala) fusion peptide analog of NEMO-Binding Domain Binding peptide (Catalogue No. 480025) that serves as a negative control.
500 µg 480030
NEMO NOA Pepotide, Cell-Permeable, A-UBI (NEMO Ubiquitin Binding Domain Inhibitor)
A cell-permeable 37-mer peptide derived from the NEMO NOA ubiquitin binding site that attenuates NF-κB activation via blocking interactions between K63-polyubiquitin chains and the NOA ubiquitin binding domain of NEMO (Kd = 17 µM for CC2-LZ/A-UBI complex).
2 mg 481418
NF-κB Activation Inhibitor 6-Amino-4-(4-phenoxyphenylethylamino)quinazoline
A cell-permeable quinazoline compound that acts as a potent inhibitor of NF-κB transcriptional activation (IC50 = 11 nM in Jurkat cells).
1 mg 481406
36
NF-κB Activation Inhibitor V, 5HPP-33 (5-Hydroxy-(2,6-diisopropylphenyl)-1H-isoindole-1,3-dione)
A cell-permeable N-phenylpthalimide compound that inhibits IL-1-induced NF-κB translocation in HeLa cells (IC50 = 530 nM). Effectively suppresses the proliferation of various cancer cells, including several Paclitaxel-resistant lines.
25 mg 100066
NF-κB Activation Inhibitor VI, BOT-64 (6,6-Dimethyl-2-(phenylimino)-6,7-dihydro-5H-benzo[1,3]
oxathiol-4-one)
A cell-permeable benzoxathiole compound that acts as an IKK-2 inhibitor, presumably by targeting the Ser177 and/or Ser181 residues in the kinase's activation loop domain.
5 mg 481414
NF-κB Activation Inhibitor VII, CID-2858522 (1-(3,5-di-tert-butyl-4-hydroxyphenyl)-2-(2-(3-
hydroxypropylamino)-5,6-dimethyl-1H-benzo[d]imidazol-1-yl)
ethanone;)
A cell-permeable, selective inhibitor of PMA-induced NF-κB activation PKC pathway activators such as PMA (IC50 = 70 nM).
10 mg 480457
NF-κB Activation Inhibitor VIII, EVP4593 (N4-(4-phenoxyphenethyl)quinazoline-4,6-diamine,
SOC Inhibitor)
A cell-permeable quinazoline derivative that blocks PMA/PHA-induced NF-κB activation (IC50 = 11 nM).
5 mg 481417
NF-κB SN50, Cell-Permeable Inhibitor Peptide (AAVALLPAVLLALLAPVQRKRQKLMP)
Contains the nuclear localization sequence (NLS) of the transcription factor NF-κB p50 linked to the hydrophobic region (h-region) of the signal peptide of Kaposi �broblast growth factor (K-FGF). The peptide N-terminal K-FGF h-region confers cell-permeability, while the NLS (360-369) inhibits translocation of the NF-κB into the nucleus.
500 µg1 mg
481480
NF-κB SN50M, Cell-Permeable Inactive Control Peptide (AAVALLPAVLLALLAPVQRNGQKLMP)
An inactive control for SN50 peptide (Catalogue No. 481480). Corresponds to the SN50 peptide sequence with substitutions of Lys363 for Asn and Arg364 for Gly in the NLS region.
500 µg 481486
Oridonin, R. rubescens A cell-permeable inhibitor of NF-κB activity that displays anti-proliferative (ED50 ~ 2.7 µg/mL in lymphoid malignant cells) and anti-angiogenic properties. Inhibits p65 NF-κB transcriptional activity by disrupting NF-κB DNA-binding activity without interfering with its nuclear translocation
5 mg 496915
(5Z)-7-Oxozeaenol, Curvularia sp. A cell-permeable fungal resorcylic lactone that acts as a selective, highly potent, ATP-comeptitive, irreversible inhibitor of MAPKKK TAK1 activity (IC50 = 8 nM).
1 mg 499610
Parthenolide, Tanacetum parthenium A sesquiterpene lactone with anti-in�ammatory, antisecretory, and spasmolytic properties that also inhibits NF-κB and activation of MAP kinase.
50 mg 512732
Pristimerin A cell-permeable dienone-phenolic triterpenoid that blocks proteasome chymotrypsin subunit (IC50 = 2.2 and 3.0 µM against puri�ed rabbit 20S and human 26S proteasome, respectively) and cellular NF-κB pathway/IKK activation.
5 mg 530070
Ro106-9920 A cell-permeable tetrazolopyridazine-phenylsulfoxide compound that acts as a highly selective, irreversible inhibitor of IκBαee ubiquitination (IC50 = 2.3 µM). Also reported to prevent degradation of IκBαee in MM6 cells (complete inhibition at ~3-5 µM).
1 mg5 mg
557550
TIRAP Inhibitor Peptide, Cell-Permeable (Ant-Tirap138-151)
A synthetic, cell-permeable peptide that corresponds to mouse toll-interleukin 1 receptor (TIR) domain-containing adapter protein 138-151 (TIRAP) fused to the Drosophila antennapedia sequence. Shown to speci�cally inhibit LPS-induced NF-κB activation. Also reported to block IκBα degradation.
1 mg 613570
TIRAP Inhibitor Peptide, Control, Cell-Permeable (Ant-Tirap151-138)
A cell-permeable synthetic peptide containing mouse toll-interleukin 1 receptor (TIR) domain-containing adapter protein 151-138 reverse sequence (TIRAP; also called Mal (MyD88-adapter-like), fused to the Drosophila antennapedia sequence. Serves as a control for TIRAP Inhibitor Peptide (Catalogue No. 613570).
1 mg 613571
(5Z)-Zeaenol, Curvularia sp. A cell-permeable resorcylic acid lactone compound that may serve as a negative control for TAK1 (TGF-β-Activated Kinase-1) inhibition studies.
1 mg 499609
Description Comments Qty/Pk Catalogue No.
InSolution™ NF-κB Activation Inhibitor A 10 mM (1 mg/281 µL) solution of NF-κB Activation Inhibitor (Catalogue No. 481406) in DMSO.
1 mg 481407
NF-κB Activation Inhibitor II, JSH-23 (4-Methyl-N1-(3-phenylpropyl)benzene-1,2-diamine)
A cell-permeable, selective blocker of the nuclear translocation of NF-κB p65 that does not affect IκB degradation (IC50 = 7.1 µM in LPS-stimulated macrophages RAW 264.7 stably transfected with NF-κB-SEAP-NPT). Shown to suppress DNA-binding of NF-κB.
5 mg 481408
NF-κB Activation Inhibitor III (3-Chloro-4-nitro-N-(5-nitro-2-thiazolyl)-benzamide, SM-7368)
A cell-permeable inhibitor of TNF-α-stimulated NF-κB activation and subsequent upregulation of MMP-9 in HT1080 cells (complete inhibition at 10 µM).
5 mg 481411
NF-κB Activation Inhibitor IV ((E)-2-Fluoro-4’-methoxystilbene)
A cell-permeable trans-stilbene Resveratrol (Catalogue No. 554325) analog that is shown to be ~130-fold more potent than Resveratrol in inhibiting TNF-α-stimulated NF-κB reporter activity in 293T cells (IC50 = 150 nM).
10 mg 481412
ANTI-PHOSPHO-AKT1 (Y326); 50 UG
ANTI-PHOSPHO-AKT (THR308), CLONE 50-1C-25; 100 UL
ANTI-AKT/PKB, PH DOMAIN, CLONE SKB1, ALEXA FLUOR(R) 488 CONJUGATE; 50 UG
ANTI-AKT/PKB, PH DOMAIN, CLONE SKB1, ALEXA FLUOR(R) 555 CONJUGATE; 50 UG
ANTI-AKT3/PKBGAMMA, CLONE GMA104; 100 UG
ANTI-AKT/PKB, PH DOMAIN, CLONE SKB1; 100 UG
ANTI-AKT1/PKBALPHA, CLONE AW24; 100 UL
ANTI-AKT3/PKBGAMMA; 100 UL
ANTI-PHOSPHO-AKT (SER473), CLONE 6F5; 100 UG
ANTI-PHOSPHO-AKT1/PKBALPHA (SER473), CLONE SK703; 100 UL
ANTI-PHOSPHO-AKT1/PKBALPHA (THR308), CLONE NL50; 100 UL
ANTI-PHOSPHO-AKT1/PKBALPHA (SER473), CLONE 11E6; 100 UG
ANTI-AKT2/PKBBETA, CLONE AW114; 100 UL
ANTI-AKT2/PKBBETA, CLONE AW114; 100 UL
ANTI-AKT2/PKBBETA; 200 UG
ANTI-PHOSPHO-AKT (THR34); 100 UL
ANTI-PHOSPHO-AKT1/PKBALPHA (SER473), CLONE SK703; 100 UG
ANTI-PHOSPHO-AKT1/PKBALPHA (THR308), CLONE NL50; 100 UL
ANTI-AKT1/PKBALPHA, CLONE AW24; 100 UL
ANTI-PHOSPHO-AKT1 (TYR326); 100 UG
ANTI-PHOSPHO-AKT (THR308); 100 UL
ANTI-PHOSPHO-AKT (TYR450); 100 UG
ANTI-PI3 KINASE, P110DELTA RABBIT MONOCLONAL; 100 UL
ANTI-PI3 KINASE ALPHA, CLONE 2B12.1; 100 UG
ANTI-PI3 KINASE P85 ANTIBODY, N-SH2 DOMAIN , CAT. NO. ABS233
ANTI-PI3 KINASE, P110ALPHA; 200 UG
ANTI-PI3 KINASE, P110GAMMA; 100 UL
ANTI-PI3 KINASE, P110ALPHA RABBIT MONOCLONAL; 100 UL
ANTI-PI3K TYPE 3, CLONE EPR5301, RABBIT MONOCLONAL
ANTI-PI3 KINASE, P85ALPHA RABBIT MONOCLONAL; 100 UL
ANTI-PI3 KINASE, P85, N-SH2, CLONE UB93-3; 200 UL
ANTI-PI3 KINASE ANTIBODY, P85 , CAT. NO. ABS234
ANTI-PI3 KINASE, P85; 125 UL
ANTI-PI3 KINASE, P110DELTA, CLONE AW103; 200 UG
ANTI-PI3 KINASE, P110BETA, RABBIT MONOCLONAL; 100 UL
ANTI-PI3 KINASE, P110ALPHA; 100 UG
ANTI-PI3 KINASE, P110BETA;100 UL
ANTI-PI3 KINASE BETA, P110B, CLONE 8C3.2; 100 UG
ANTI-PI3 KINASE, P110GAMMA, CLONE 17D7.2; 100 UG
ANTI-PI3-KINASE TYPE 3, CLONE 17D1.1; 100 UG
ANTI-PI3 KINASE-C2-ALPHA, CLONE 9H4.2; 100 UG
ANTI-PI3 KINASE-C2BETA; 100 UG
ANTI-PI3K TYPE 3
ANTI-PHOSPHO-RAF-1 (SER338); 100 UG
ANTI-RAF-1, CLONE AM223; 100 UL
ANTI-PHOSPHO-RAF-1 (TYR340/TYR341); 100 UL
ANTI-RAF-1, RABBIT MONOCLONAL; 100 UL
ANTI-PHOSPHO-RAF-1 (SER338); 200 UG
ANTImTOR/FRAP (AB-2) mouse MAB (22C2)
ANTImTOR, CLONE F11; 100 UG
ANTImTOR, RABBIT MAB; 100 UL
ANTI-PHOSPHOmTOR (SER2448)
ANTI-PHOSPHOmTOR (SER2481); 100 UL
ANTI-PHOSPHOmTOR (THR2446); 100 UL
ANTImTOR, A.A.1223-1290; 100 UG
ANTImTOR, CLONE 21A12.2; 100 UG
ANTImTOR, CLONE 2ID8.2; 100 UG
ANTImTOR/FRAP, CLONE EPR390(N), RABBIT MONOCLONAL
ANTImTOR/FRAP, CLONE 22C2
ANTI-PHOSPHOmTOR (SER2159); 100 UL
ANTI-PHOSPHOmTOR (THR2164)
ANTI-P70 S6 KINASE; 200 UG
ANTI-PHOSPHO-P70 S6 KINASE (THR389)
ANTI-P70 S6 KINASE (S6K); 200 UG
ANTI-PHOSPHO-P70 S6 KINASE (THR421/SER424), RABBIT
MONOCLONAL; 100 UL
ANTI-PHOSPHO-P70 S6 KINASE (THR389)
ANTI-P70 S6 KINASE, RABBIT MONOCLONAL; 100 UL
ANTI-PHOSPHO-P70S6K (THR470)
ANTI-P70 S6 KINASE, CLONE 20-10C-6; 100 UL
大鼠;小鼠;鸡;马;犬;牛
人;小鼠;兔;牛;鸡
人;小鼠;大鼠
人;小鼠;大鼠
人
人;小鼠;大鼠
人;小鼠;大鼠
人
人;小鼠;大鼠;脊椎动物
人;小鼠
人;小鼠
小鼠;大鼠;人;犬
人;小鼠;大鼠
人;大鼠
人;小鼠;大鼠
人
人;小鼠
人;小鼠;牛;鸡;大鼠
人;小鼠;大鼠
人;小鼠;大鼠;犬;非洲爪蟾
人;小鼠;大鼠;牛;非洲爪蟾;犬
小鼠;大鼠;人;牛;鸡;马
人
小鼠
人;大鼠
人;脊椎动物
人
人
人;小鼠;大鼠
人;小鼠;大鼠
人;小鼠;大鼠
人;大鼠
人;小鼠;猴子;大鼠
人
人
人;大鼠
人;猴子;犬;小鼠;大鼠
大鼠;人;小鼠
人;小鼠;大鼠
人;小鼠;大鼠
小鼠;大鼠;人
人;黑猩猩;恒河猴
人;小鼠
小鼠
人;小鼠;大鼠
人
人;小鼠;大鼠
小鼠
人
人;大鼠
人;小鼠;大鼠
人
人;大鼠
人;小鼠;大鼠
人;大鼠
人
人;小鼠
人
人
人;小鼠;大鼠;羊;斑马鱼
人
人;小鼠;大鼠
人;小鼠
人;小鼠;大鼠
人
人
人;小鼠;大鼠
人
人;小鼠;大鼠
WB
WB;IF;ICC;ELISA
FC;ICC;IF;WB
FC;ICC;IF;WB
IP;WB
FC;ICC;IP;WB
IP;WB
IP;WB
ELISA;WB;FC;IF;IHC
WB
WB
ELISA;WB
WB;IP
IP;WB
IP;WB
WB
WB
WB
WB;IP
WB
IHC(P)
WB;Enzyme Assay
IF;IP;WB
WB
WB;IP
IP
FC;IP;WB
IF;IP;WB
WB;IHC
FC;ICC;IF;IHC(P);IP;WB
WB;IP
WB;IP
IP;WB
WB
FC;IP;WB
WB;IP;IHC;ELISA
WB;IP;ICC;IHC;ELISA
WB;ICC
WB;ICC
WB;ICC
WB;ICC
WB;IHC
ICC
WB
IP;WB
WB
IF;IHC(P);IP;WB
WB
WB;IP
WB;IP
FC;ICC;IHC(P);IP;WB
WB
WB
WB
WB;IP;IHC(P)
WB;ICC
WB;ICC
WB
WB;IP
WB;Peptide Inhibition
Assay;Dot Blot
Dot Blot;WB
IP;WB
WB;ICC
WB
IP;WB
WB;Peptide Inhibition Assay
FC;IHC(P);IP;WB
WB
WB;IP;ICC
50UG
100UL
50UG
50UG
100UG
100UG
100UL
100UL
100UG
100UL
100UL
100UG
100UL
100UL
200UG
100UL
100UG
100UL
100UL
100UG
100UL
100UG
100UL
100UG
100UG
200UG
100UL
100UL
100uL
100UL
200UL
200UL
125UL
200UG
100UL
100UG
100UL
100UG
100UG
100UG
100UG
100UG
100ug
100UG
100UL
100UL
100UL
200UG
100ug
100UG
100UL
100UG
100UL
100UL
100UG
100UG
100UG
100uL
100UG
100uL
100UL
200UG
100ug
200UG
100UL
100UG
100UL
200UL
100UL
09-288
05-802R
16-293
16-294
05-780
05-591
05-796
07-383
05-1003
04-736
05-802
05-669
04-771
05-771
07-372
07-789
05-736
04-802
04-796
AB9927
07-1398
07-1643
04-401
MABS76
ABS233
07-658
04-402
04-399
MABN1114
04-403
05-217
ABS234
06-195
05-703
04-400
09-481
09-482
05-1558
05-1559
05-1563
05-1560
07-2170
07-2186
05-534
04-739
07-815
04-412
05-538
OP97-100UG
MABS14
04-385
09-213
09-343
09-345
07-1415
05-1564
05-1592
MABS799
MABS196
ABS79
ABS88
07-402
MABS82
06-926
04-393
07-018-I
04-391
ABS431
05-781R
37
Oxidative Stress Pathway
Caspase-9 Caspase-3Apaf-1
ONOO-
NO•
NOS
SOD
GSHPeroxidase
GSHSynthase
GSHReductase
GSSG
GSH
Fe2+
Fe3+
Glycine
CysteineGlutamate
γ-glutamyl CysteineSynthase
NADP+
NADPH
L-arginine
CytC
Nuc
leus
Cell
Mem
bran
e
Apoptosis
ProteinOxidation
CellularDysfunction
Nitrosative StressInflammation
DNA Damage
CellDeath
LipidPeroxidation
(MDA)
ATP
∆ΨmMitochondrialDamage
CarbonylFormation (PCC)
Fenton Reaction
Telomere Attrition
Fe2+
Fe3+
OH•
O2
H+
H+
O2
O2
H2O
+
O2
H2O+
OH•
H2O2
H2O2
O2•-
O2•-O2
•-
O2•-
O2•-
I xelpmoC
III xel pmoC
II xel pmoC
VI xel pmoC
PTAesaht nyS
Inner Mitochondrial Membrane
H+
H+ H+H+H+
O2
H+
H+
ROS
ADP+Pi
ATP
氧化应激是指体内氧化与抗氧化作用失衡,倾向于氧化,导致中性粒细胞炎性浸润,蛋白酶分泌增加,产生大量氧化中间产
物。氧化应激是由自由基在体内产生的一种负面作用,并被认为是导致衰老和疾病的一个重要因素。自由基的一个主要功能是
激活宿主的免疫反应。炎症细胞产生的自由基和强氧化物对于杀死入侵的微生物具有重要的作用。然而,自由基的过量产生也
会引发细胞的致病过程。氧化应激水平升高诱发的最常见疾病有心脏病、癌症、骨关节炎、风湿性关节炎、糖尿病以及神经退
化性问题如阿尔兹海默症、帕金森病等。因此,了解自由基引发的氧化应激通路是十分重要的。
AB5605AB5830MAB3560
AB9890
07-520
MAB5310AB3282
MABN527ABS97MABC71307-685
MABN457
06-984
ABC391AB5380AB5382
AB16301AB553205-233
16-226
16-207
16-310
07-61007-35707-56107-562AB5484MAB5328
ABC245MABC684
AB10346AB5482AB9328
Anti-4-Hydroxynonenal AntibodyAnti-8-Hydroxydeoxyguanosine AntibodyAnti-8-Oxoguanine Antibody, clone 483.15
Anti-AGE (Advanced Glycated End-products) AntibodyAnti-eNOS/NOS III Antibody, CT
Anti-Glutathione Antibody, clone D8Anti-Glutathione-S-Transferase Antibody, S. japonicum formAnti-iNOS/NOS II, clone 13F5.1 AntibodyAnti-KEAP1 AntibodyAnti-KEAP1, clone 7G4B10 AntibodyAnti-Lactotransferrin Antibody
Anti-Mitochondrial dicarboxylate carrier Antibody, clone 1F5.1Anti-Mn-SOD Antibody
Anti-NDUFB7 AntibodyAnti-Nitric Oxide Synthase I AntibodyAnti-Nitric Oxide Synthase II Antibody
Anti-Nitric Oxide Synthase III AntibodyAnti-Nitrotyrosine AntibodyAnti-Nitrotyrosine Antibody, clone 1A6
Anti-Nitrotyrosine Antibody, clone 1A6, Alexa Fluor® 488 conjugateAnti-Nitrotyrosine Antibody, clone 1A6, HRP conjugateAnti-Nitrotyrosine Magnetic Bead Conjugate AntibodyAnti-Peroxiredoxin 2 AntibodyAnti-phospho-eNOS/NOS III (Ser116) AntibodyAnti-phospho-eNOS/NOS III (Ser617) AntibodyAnti-phospho-eNOS/NOS III (Ser635) AntibodyAnti-RAGE AntibodyAnti-RAGE Antibody, clone DD/A11 or A11
Anti-REDD1 AntibodyAnti-SOD1 Antibody, clone 6F5
Anti-SOD2 AntibodyAnti-Superoxide Dismutase 1 AntibodyAnti-Thioredoxin 1 Antibody
ELISA;Western BlottingELISA;Immunohistochemistry (Paraffin)ELISA;Immunocytochemistry;Chromatin Immunoprecipitation (ChIP)ELISA;Western Blotting
Western Blotting
Western BlottingELISA;Western Blotting
Western Blotting;ImmunohistochemistryWestern Blotting;ImmunocytochemistryImmunohistochemistry;Flow Cytometry;ELISAImmunoprecipitation;Western Blotting;Immunocytochemistry;Immunohistochemistry;Immunohistochemistry (Paraffin)Western Blotting;Immunohistochemistry
Immunohistochemistry;Immunohistochemistry (Paraffin);Western BlottingWestern Blotting;ImmunohistochemistryImmunohistochemistry;Western BlottingImmunocytochemistry;Immunohistochemistry;Western BlottingWestern Blotting;ImmunocytochemistryELISA;ImmunohistochemistryImmunocytochemistry;Immunohistochemistry;Immunoprecipitation;Western BlottingFlow Cytometry;Western Blotting
ELISA;Western Blotting
Immunoprecipitation
Western Blotting;ImmunocytochemistryWestern BlottingWestern BlottingWestern BlottingELISA;Immunohistochemistry (Paraffin)ELISA;Immunocytochemistry;Immunohistochemistry;Western BlottingWestern Blotting;Immunohistochemistry (Paraffin)Western Blotting;Immunocytochemistry;Flow CytometryWestern BlottingWestern BlottingWestern Blotting
HumanHumanHuman;Rat;Mouse;Monkey
Human
Human;Rat;Bovine;Mouse;Canine;Guinea Pig;PigEukaryoteVertebrates;Schistosoma japonicum
HumanHuman;Mouse;RatHumanHuman;Mouse;Rat;Hamster;Monkey
Mouse;Rat
Human;Rat;Mouse;Bovine
Human;MouseHuman;Mouse;Monkey;Rat;SheepMouse;Rat
HumanAllVertebrates
Vertebrates
All
All
HumanBovine;HumanBovineBovineHumanBovine;Human;Mouse
Human;Mouse;RatHuman;Mouse
Human;Rat;MouseMouse;RatMouse;Rat
200 µL200 µL100 µL
200 µL
200 µg
100 µg500 µg
100 µL100 µg100 µg100 µL
100 µg
200 µg
150 µL50 µL50 µL
100 µg100 µL100 µg
100 µg
50 µL
200 µL
50 µL100 µL200 µL200 µL200 µL100 µg
100 µg100 µL
50 µL50 µL100 µL
DescriptionCat. No. Key Applications Species Reactivity Qty/Pk
抗体
38
试剂盒
Milliplex kit
Small Molecules7-Nitroindazole 483400AAPH 100100AG126 658452α -Lipoic Acid 437692Apocynin 178385Carboxy-PTIO, Sodium Salt 217386Diphenyleneiodonium Chloride 300260DL-α -Difluoromethylornithine, Hydrochloride 288500DPPH Free Radical 300267FeTPPS 341492Keap1-Nrf2 Interaction Probe, ML334 505987L-NIL, Dihydrochloride 482100MCI-186 443300Methylene Blue 467250MnTMPyP 475872NG,NG-Dimethyl-L-arginine, Dihydrochloride 311203NG-Hydroxy-L-arginine, Monoacetate Salt 399250Nitric Oxide Synthase, Neuronal Inhibitor I 490070nNOS - PSD-95 Interaction Inhibitor, ZL006 482740NOX Inhibitor VIII, VAS3947 532336NOX1 Inhibitor, ML171 492002Nrf2 Activator 492040NS 2028 492030SKF-525A, Hydrochloride 567300
Description Cat. No.Description Cat. No.
OxyBlot™ Protein Oxidation Detection Kit S7150OxyELISA™ Oxidized Protein Quantitation Kit S7250OxyICC™ Oxidized Protein Detection Kit S7350OxyIHC™ Oxidized Protein Detection Kit S7450
AB5605AB5830MAB3560
AB9890
07-520
MAB5310AB3282
MABN527ABS97MABC71307-685
MABN457
06-984
ABC391AB5380AB5382
AB16301AB553205-233
16-226
16-207
16-310
07-61007-35707-56107-562AB5484MAB5328
ABC245MABC684
AB10346AB5482AB9328
Anti-4-Hydroxynonenal AntibodyAnti-8-Hydroxydeoxyguanosine AntibodyAnti-8-Oxoguanine Antibody, clone 483.15
Anti-AGE (Advanced Glycated End-products) AntibodyAnti-eNOS/NOS III Antibody, CT
Anti-Glutathione Antibody, clone D8Anti-Glutathione-S-Transferase Antibody, S. japonicum formAnti-iNOS/NOS II, clone 13F5.1 AntibodyAnti-KEAP1 AntibodyAnti-KEAP1, clone 7G4B10 AntibodyAnti-Lactotransferrin Antibody
Anti-Mitochondrial dicarboxylate carrier Antibody, clone 1F5.1Anti-Mn-SOD Antibody
Anti-NDUFB7 AntibodyAnti-Nitric Oxide Synthase I AntibodyAnti-Nitric Oxide Synthase II Antibody
Anti-Nitric Oxide Synthase III AntibodyAnti-Nitrotyrosine AntibodyAnti-Nitrotyrosine Antibody, clone 1A6
Anti-Nitrotyrosine Antibody, clone 1A6, Alexa Fluor® 488 conjugateAnti-Nitrotyrosine Antibody, clone 1A6, HRP conjugateAnti-Nitrotyrosine Magnetic Bead Conjugate AntibodyAnti-Peroxiredoxin 2 AntibodyAnti-phospho-eNOS/NOS III (Ser116) AntibodyAnti-phospho-eNOS/NOS III (Ser617) AntibodyAnti-phospho-eNOS/NOS III (Ser635) AntibodyAnti-RAGE AntibodyAnti-RAGE Antibody, clone DD/A11 or A11
Anti-REDD1 AntibodyAnti-SOD1 Antibody, clone 6F5
Anti-SOD2 AntibodyAnti-Superoxide Dismutase 1 AntibodyAnti-Thioredoxin 1 Antibody
ELISA;Western BlottingELISA;Immunohistochemistry (Paraffin)ELISA;Immunocytochemistry;Chromatin Immunoprecipitation (ChIP)ELISA;Western Blotting
Western Blotting
Western BlottingELISA;Western Blotting
Western Blotting;ImmunohistochemistryWestern Blotting;ImmunocytochemistryImmunohistochemistry;Flow Cytometry;ELISAImmunoprecipitation;Western Blotting;Immunocytochemistry;Immunohistochemistry;Immunohistochemistry (Paraffin)Western Blotting;Immunohistochemistry
Immunohistochemistry;Immunohistochemistry (Paraffin);Western BlottingWestern Blotting;ImmunohistochemistryImmunohistochemistry;Western BlottingImmunocytochemistry;Immunohistochemistry;Western BlottingWestern Blotting;ImmunocytochemistryELISA;ImmunohistochemistryImmunocytochemistry;Immunohistochemistry;Immunoprecipitation;Western BlottingFlow Cytometry;Western Blotting
ELISA;Western Blotting
Immunoprecipitation
Western Blotting;ImmunocytochemistryWestern BlottingWestern BlottingWestern BlottingELISA;Immunohistochemistry (Paraffin)ELISA;Immunocytochemistry;Immunohistochemistry;Western BlottingWestern Blotting;Immunohistochemistry (Paraffin)Western Blotting;Immunocytochemistry;Flow CytometryWestern BlottingWestern BlottingWestern Blotting
HumanHumanHuman;Rat;Mouse;Monkey
Human
Human;Rat;Bovine;Mouse;Canine;Guinea Pig;PigEukaryoteVertebrates;Schistosoma japonicum
HumanHuman;Mouse;RatHumanHuman;Mouse;Rat;Hamster;Monkey
Mouse;Rat
Human;Rat;Mouse;Bovine
Human;MouseHuman;Mouse;Monkey;Rat;SheepMouse;Rat
HumanAllVertebrates
Vertebrates
All
All
HumanBovine;HumanBovineBovineHumanBovine;Human;Mouse
Human;Mouse;RatHuman;Mouse
Human;Rat;MouseMouse;RatMouse;Rat
200 µL200 µL100 µL
200 µL
200 µg
100 µg500 µg
100 µL100 µg100 µg100 µL
100 µg
200 µg
150 µL50 µL50 µL
100 µg100 µL100 µg
100 µg
50 µL
200 µL
50 µL100 µL200 µL200 µL200 µL100 µg
100 µg100 µL
50 µL50 µL100 µL
DescriptionCat. No. Key Applications Species Reactivity Qty/Pk
抑制剂
Human Oxidative Stress(Cat.No.HØXSTMAG-18K)CatalasePeroxiredoxin 2(PRX2/PRDX2)Superoxide dismutase 1(SOD1)Superoxide dismutase 2(SOD2)Thioredoxin(TRX1)
Human Oxidative Phosphorylation(Cat.No.HØXSMAG-16K)Complex Ⅰ(NADH-Ubiquione Oxidoreductase)Complex Ⅱ(Succinate Ubiquione Oxidoreductase)Complex Ⅲ(Ubiquione Cytochrome C Oxidoreductase)Complex Ⅳ(Cytochrome C Oxidase)Complex Ⅴ(ATP Synthase)NNT(Nicotinamide Nucleotide Transhydrogenase
Rat/Mouse Oxidative Phosphorylation(Cat.No.RMHØXSMAG-17K)Complex Ⅰ(NADH-Ubiquione Oxidoreductase)Complex Ⅲ(Ubiquione Cytochrome C Oxidoreductase)Complex Ⅴ(ATP Synthase)
39
P53 SignalingP53是一个肿瘤抑制蛋白,调节各种各样基因的表达,包括细胞凋亡,生长抑制,抑制细胞周期进程,分化和加速DNA修复,基因毒性和细胞应激后的衰老等。在所有肿瘤中p53是最频繁突变的蛋白,据估计在60%的肿瘤中有变异形式,影响其生长抑制活动。一些常见的肿瘤有较高的发病率,90%的子宫癌和 70%的大肠癌中发现p53突变。
p53基因是研究最透彻,功能最强大的一种抑癌基因。野生型p53对细胞周期和凋亡起关键性作用,尤其是对受照射、细胞毒制剂、热疗打击的癌细胞,起更大的杀伤作用。其主要作用为:抑制并杀灭肿瘤细胞;与放、化疗手段协同,增强其杀灭癌细胞的功效,达到1+1大于2的效果;抑制肿瘤血管生成,有效防止肿瘤的复发、转移等。
由于P53可以阻滞肿瘤细胞G1/G0期,使肿瘤细胞不能进入S期,从而抑制肿瘤细胞增生。近年来,人们不断探索应用P53基因对肿瘤进行治疗,主要应用重组腺病毒-P53(rAd-P53)进行肿瘤临床研究。目前食管癌、卵巢癌、膀胱癌、非小细胞肺癌、乳腺癌等临床试验已取得阶段性成果,让人类在抗击肿瘤的斗争中看到了一丝曙光。
InhibitorSelect™ p53 PathwayRegulators Panel (Catalogue No. 506169)
Description Qty/Pk Catalogue No.
ATM Kinase Inhibitor 2 mg 118500
ATM/ATR Kinase Inhibitor 5 mg 118501
Anacardic Acid 10 mg 172050
Cdk1 Inhibitor IV, RO-3306 5 mg 217699
Casein Kinase II Inhibitor III, TBCA
5 mg 218710
Chk2 Inhibitor II 1 mg 220486
Hdm2 E3 Ligase Inhibitor 5 mg 373225
JNK Inhibitor II 5 mg 420119
MDM2 Antagonist, Nutlin-3, Racemic
5 mg 444143
MDMX Inhibitor, SJ-172550 10 mg 444155
MG-132 5 mg 474790
p21-Activated Kinase Inhibitor III, IPA-3
5 mg 506106
Pi�thrin-α 1 mg 506132
PI-103 5 mg 528100
PARP Inhibitor VIII, PJ34 1 mg 528150
PPM1D Phosphatase Inhibitor 10 mg 529578
PRIMA-1 10 mg 530050
Caspase Inhibitor I 1 mg 627610
Trichostatin A, Streptomyces sp. 1 mg 647925
Anhydrous DMSO 15 mL KP31817
The Inhibitor panel includes:
Growth FactorsIonizingRadiationChemotherapyUVHypoxia
RTKs
JNKHIPK2CSNK1δ
p38
CKII
Chk1
ATR
DyrkCKI
CDK1
ATM
PAK4
AKT
S6K1
AMPK
mTOR
mTORC2
mTORC1
Chk2
DNA-PK
p53 Degradation
MDM2 Degradation
Apoptosis
Translation
Cell CycleProgression
DNA Damage
Premature Senescence
p53
p53
c-Abl
Caspase 6,7
PARP
HDAC
HAT
Proteasome
MDM2
p21CIP1
MDM2
CDC42
TSC2
MDMX
Cyclin D1
CyclinB
CDK2
CyclinE
Rb
Rb
WIP1TSC1
RHEB
GbLRictor
mTORGbLRictor
PI 3-K
E2F
CDK4
E2F
p53
p53Mutant
• p21-Activated Kinase Inhibitor III, IPA-3
• PARP Inhibitor VIII, PJ34
• Chk2 Inhibitor II
• ATM Kinase Inhibitor• ATM/ATR Kinase Inhibitor
• MG-132
• Casein Kinase II Inhibitor III, TBCA
• Anacardic Acid
• ATM/ATR Kinase Inhibitor
• JNK Inhibitor II, SP600125
• JNK Inhibitor II, SP600125
• JNK Inhibitor II,SP600125
• PPM1D Phosphatase Inhibitor
• Caspase Inhibitor I
• Cdk1 Inhibitor IV, RO-3306
• PRIMA-1
• MDMXInhibitor,
SJ-172550
• Trichostatin A, Streptomyces sp.
• Hdm2 E3 Ligase Inhibitor
• MDM2 Antagonist, Nutlin-3, Racemic
• MDM2 Antagonist,Nutlin-3, Racemic
• Pi�thrin-α
• JNK II,SP600125
• PI-103
• PI-103
• PI-103
Ac
Phosphorylation
Ubiquitin
Calbiochem® Inhibitors*以上组分可按目录号单独购买
40
Description Species Applications Catalogue No.
Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421) Human, monkey, mouse, rabbit, rat OP03
Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421) Human, monkey, mouse, rabbit, rat OP03L
Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421) Human, monkey, mouse, rabbit, rat FC, FS, IF OP03F
Anti-p53 (Ab-2) (Pantropic) Mouse mAb (PAb1801) Human OP09
Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1) Feline, human OP43
Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1) Feline, human OP43L
Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1) Agarose Conjugate Feline, human OP43A
Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1) Fluorescein Conjugate Feline, human OP43F
Anti-p53 (Ab-7) (Pantropic) Sheep pAb Human, mouse, rat PC35
Anti-p53 (Ab-11) (Pantropic) Mouse mAb (PAb1802) Human, mouse IB, IP OP104L
Anti-p53 (Ab-12) (Pantropic) Mouse mAb (DO-7) Bovine, human, monkey OP140
Anti-p53 (pantropic), clone DO-1 Human IB, ICC, IHC, IF, IP MABE327
Anti-p53 R2 Human, mouse, rat IB AB4052
Anti-p53, Clone Pab421 Mouse IB, IP, IH MABE283
Anti-p53 Antibody, clone E26 Human, rat IB, IC, IH 04-241
Anti-p53 Antibody, clone E26 Human, rat IB, IC, IH Ab9985
Anti-p53 Antibody, aa 211-220, clone240 Bovine, chicken, human, hamster, mouse, monkey, rat
IB, IH, IP CBL404
Anti-p53 Antibody, clone BP53-12 Human IB, IH, IP 05-224
Anti-p53 (Ab-3) (Mutant) Mouse mAb (PAb240) Bovine, chicken, hamster, human, mouse, rat OP29
Anti-p53 (Ab-3) (Mutant) Mouse mAb (PAb240) Bovine, chicken, hamster, human, mouse, rat OP29L
Anti-p53 (Ab-4) (Wild type) Mouse mAb (PAb246) Mouse, rat OP32
Anti-p53 (Ab-4) (Wild type) Mouse mAb (PAb246) Mouse, rat OP32L
Anti-p53 (Wild type), Clone Pab1620 Human, mouse IB, IH MABE339
Anti-p53 (Ab-5) (Wild type) Mouse mAb (PAb1620) Bovine, human, mouse, primate, rat. OP33
Anti-acetyl-p53 (Lys320) Antibody Bovine, chimpanzee, human IB 06-1283
Anti-acetyl-p53 (Lys373) Antibody, clone EP356(2)AY Human IB, IC, IH(P) 04-1137
Anti-acetyl-p53 (Lys382) Antibody, clone EPR358(2), Rabbit Human IB, IC 04-1146
Anti-acetyl-p53 Antibody (Lys373, Lys382) Most vertebrates IB 06-758
Anti-acetyl-p53 (Lys373) Antibody Human IB, IP 06-916
PhosphoDetect™ Anti-p53 (pSer15) (Ab-3) Rabbit pAb Human, mouse, rat PC386
PhosphoDetect™ Anti-p53 (pSer15) (Ab-6) Rabbit pAb Human PC461
PhosphoDetect™ Anti-p53 (pSer20) Rabbit pAb Human. mouse DR1023
PhosphoDetect™ Anti-p53 (pSer392) (Ab-4) Rabbit pAb Human, mouse IB PC387
PhosphoDetect™ Anti-p53 (pSer392) Mouse mAb (9F4) Human, mouse ELISA, IB 506133
PhosphoDetect™ Anti-p53 (pSer46) Rabbit pAb Human DR1024
Anti-phospho-p53 (Ser33) Antibody, clone EP2393Y Human IB, IC MABE199
Anti-phospho-p53 (Ser6) Antibody, clone Y179 Human IB, IC, IH, IP 04-540
Anti-phospho-p53 (Ser392) Antibody, clone EP155Y Human, rat IB, IC, IH(P), IC, IP 04-244
Anti-p53 (C-terminus) Antibody, clone E47 Human FC, IB, ICC, IP 04-242
Anti-p53 (N-term) Antibody, clone Y5 Human, rat IB, IC, IH(P), IP 04-1083
Anti-p53 Binding Protein 1 (Ab-1) Rabbit pAb Human, mouse PC712
Anti-p53 Binding Protein 1 Mouse mAb (BP13) Human DR1003
Anti-TP53BP2 Mouse mAb (3F8) Human ELISA, IB, IH (P) AP1163
*ELISA: enzyme linked immunosorbent assay; FC: �ow cytometry; FFS: free �oating sections; FS: frozen sections; GS: gel shift; IB: immunoblotting, IC: immunocytochemistry; IF: immuno�uorescence; IH: immunohistochemistry IP: immunoprecipitation; PS: paraf�n sections
P53抗体
Description Species Applications Catalogue No.
Anti-p53 Antibody, aa 211-220, clone240 Bovine, chicken, human, hamster, mouse, monkey, rat
IB, IH, IP CBL404
Anti-p53 Antibody, clone BP53-12 Human IB, IH, IP 05-224
Anti-p53 (Ab-3) (Mutant) Mouse mAb (PAb240) Bovine, chicken, hamster, human, mouse, rat OP29
Anti-p53 (Ab-3) (Mutant) Mouse mAb (PAb240) Bovine, chicken, hamster, human, mouse, rat OP29L
Anti-p53 (Wild type), Clone Pab1620 Human, mouse IB, IH MABE339
Anti-p53 (Ab-5) (Wild type) Mouse mAb (PAb1620) Bovine, human, mouse, primate, rat. OP33
Anti-acetyl-p53 (Lys320) Antibody Bovine, chimpanzee, human IB 06-1283
Anti-acetyl-p53 Antibody (Lys373, Lys382) Most vertebrates IB 06-758
Anti-acetyl-p53 (Lys373) Antibody Human IB, IP 06-916
PhosphoDetect™ Anti-p53 (pSer15) (Ab-3) Rabbit pAb Human, mouse, rat PC386
PhosphoDetect™ Anti-p53 (pSer15) (Ab-6) Rabbit pAb Human PC461
Anti-phospho-p53 (Ser6) Antibody, clone Y179 Human IB, IC, IH, IP 04-540
Anti-phospho-p53 (Ser392) Antibody, clone EP155Y Human, rat IB, IC, IH(P), IC, IP 04-244
Anti-p53 Binding Protein 1 (Ab-1) Rabbit pAb Human, mouse PC712
*ELISA: enzyme linked immunosorbent assay; FC: �ow cytometry; FFS: free �oating sections; FS: frozen sections; GS: gel shift; IB: immunoblotting, IC: immunocytochemistry; IF: immuno�uorescence; IH: immunohistochemistry IP: immunoprecipitation; PS: paraf�n sections
P53抗体参考文献:
The amino-terminal transforming region of simian virus 40 large T and small t antigens functions as a J domain. Srinivasan, A, et al. Mol Cell Biol, 17:4761-73 (1997) 1997
Linear antigenic sites de�ned by the B-cell response to human p53 are localized predominantly in the amino and carboxy-termini of the protein. Legros, Y, et al. Oncogene, 9: 2071-6 (1994) 1994
Increased expression of mutant forms of p53 oncogene in primary lung cancer. Iggo, R, et al. Lancet, 335: 675-9 (1990) 1990
参考文献:
Tibbetts, R.S., et al. 1999. Genes Dev. 13, 152. Levine, A.J. 1997. Cell 88, 323. Shieh, S.Y., et al. 1997. Cell 91, 325.
参考文献:
Regulation of p53 in response to DNA damage. Lakin, N D and Jackson, S P, Oncogene, 18: 7644-55 (1999) 1999
p53, the cellular gatekeeper for growth and division. Levine, A J, Cell, 88: 323-31 (1997) 1997
p53: at the crossroads of molecular carcinogenesis and risk assessment. Harris, C C, Science, 262: 1980-1 (1993) 1993 82
41
Anti-NIK, C-terminus Human IB 100 µg AB16528
Anti-RelB Human IB, IC, IF 200 µL 07-1332
Anti-RelB Human, Mouse, Rat, Rhesus Monkey
IB, IH(P) 100 µg 06-1105
Anti-c-Rel (NF-κB) Human ELISA, EMSA, IB 100 µg 09-040
Anti-TBK1, clone AOW9 Canine, Chimpanzee, Human, Mouse
IB, IP 100 µL 04-856
Anti-TBK1/NAK (N-term), clone EP611Y Human, Mouse, Rat ICC, IB, IH(P),IP 100 µL 04-387
Anti-TBK1 Mouse (108A429) Human IB 100 µg AM76
Anti-Phospho-TBK1 (Ser172) Human, Mouse, Rat IB 100 µL 07-2192
Applications:ChIP: Chromatin
ImmunoprecipitationDB: Dot BlottingEMSA: Electrophoretic
Mobility Supershift Assay ELISA: Enzyme-linked
Immunosorbent Assay
FC: Flow Cytometry
IB: Immunoblotting
IC: Immunocytochemistry
IH: ImmunohistochemistryIP: ImmunoprecipitationPIA: Peptide Inhibition Assay
参考文献:Nuclear factor-kappaB induced by doxorubicin is de�cient in phosphorylation and acetylation and represses nuclear factor-kappaB-dependent transcription in cancer cells. Wai Chi Ho, Kathleen M Dickson, Philip A Barker. Cancer research,65:4273-81, 2005
Ineffectiveness of histone deacetylase inhibitors to induce apoptosis involves the transcriptional activation of NF-kappa B through the Akt pathway. Mayo, M. W., et al J Biol Chem, 278:18980-9, 2003
Description Species Applications Catalogue No.
Anti-MDM2 (Ab-1) Mouse mAb (IF2) Human OP46
Anti-MDM2 (Ab-2) Mouse mAb (2A10) Human OP115
Anti-MDM2 (Ab-3) Mouse (4B11) Antibody Human , mouse IB, IF, IH (P), IP OP143
Anti-MDM2 (Ab-5) Mouse mAb (4B2C1.11) Human IB, IF, IH (P), IP OP145
Anti-MDM2 (Ab-6) Mouse mAb (5B10C) Human IB, IF, IH(P), IP OP146
Human IB, IP, IC, IH, FC 04-1555
Anti-MDM2 Antibody, clone 3G9 Human, mouse IB, IP, IC, IH 04-1530
Anti-MDM4 Antibody, clone 7A8 Human, mouse IB 04-1556
Anti-MDM2, Clone IF2 Human IB, IH MABE340
Anti-MDM2, Clone 2A10 Human IB, IP MABE281
Anti-MDM2, Clone 4B2C1.11 Human. mouse IB, IC, IP MABE331
MDM2抗体
Anti-MDMx Antibody, clone 8C6
P53信号通路激活剂Description Comments Qty/Pk Catalogue No.
p53 Activator, Cell-Permeable A synthetic cell-permeable peptide corresponding to the C-terminal amino acids 361 - 382 of p53. Binds mutant p53 and restores the growth suppressor functions of p53 protein in human tumor cells.
500 µg 506131
p53 Activator II, Cell-Permeable A cell-permeable, proteolytically stable p53-activating peptide that displays antitumor properties.
500 µg 506144
p53 Activator III, RITA A cell-permeable, p53-targeting, tricyclic thiophene derivative that blocks p53-MDM2 interaction and p53 ubiquitination and induces p53-dependent apoptosis in tumor cells expressing wild-type p53.
1 mg 506149
PRIMA-1 A cell-permeable quinuclidinone analog that restores biochemical and biological function to mutant p53 and induces p53-dependent apoptosis in vitro and in vivo.
10 mg 530050
p53 Activator VII, STIMA-1 A cell-permeable compound that covalently modi�es free thiols on mutant p53 in H1299-His175 cells and fully restore its DNA binding activity to the level seen with wild-type p53, resulting in upregulation of p53-dependent gene expression.
25 mg 506168
p53 Modulator, CP-31398 A cell-permeable compound that protects wild-type p53 against heat-induced denaturation and locks newly synthesized mutant p53 in an active conformation. Unlike PRIMA-1, it directly targets the DNA binding domain of p53.
5 mg 506166
p53-Snail binding Inhibitor, GN25 A cell-permeable, speci�c p53-Snail binding inhibitor that activates p53 in a K-Ras-dependent manner and displays anti-proliferating effect toward K-Ras-transformed mouse embryonic �broblasts. Selectively activates wild-type p53 in p53WT/MT cancer cells.
25 mg 506170
Mutant p53 Reactivator, RETRA A cell-permeable compound that upregulates p53 family-responsive element-dependent reporter plasmid transcription activity in cells bearing Glu266, His273, Lys280, or Trp278 mutant p53 by increasing the cellular protein, but not mRNA, level of the p53 homologue p73 and by disrupting p73 interaction with mutant p53.
5 mg 506164
Tenovin-1 A cell-permeable compound that up-regulates cellular p53 protein, but not mRNA, level in MCF-7 (≥6-fold in 6 h), presumably by blocking MDM2-mediated p53 degradation.
10 mg 580566
RNA Ploymerase I Inhibitor II,CX-5461
5.09265.0001Inhibits RNA ploymerase I-mediated pre-rRNA transcription, but not Pol II-mediated c-myc transcription, by preventingTAF SL1 and rDNA association.
默克密理博近期发布了RNA
Polymerase I inhibitor II,
CX-5461,(cat.No.505487),
一种可渗透细胞的,高效的,
选择性和可逆的RNA聚合酶I介
导的核糖体RNA合成抑制剂,它
通过抑制合称起始阶段,但并
不影响RNA聚合酶II的活性。这
一化合物用于发表在Cancer Cell
杂志上一项十分重要的研究,
显示它在各种恶性细胞中诱导
p53依赖的细胞凋亡(在Em-Myc
淋巴瘤细胞中,IC50=5.4nM),
但是无论在野生型或非肿瘤耐
受的小鼠中,对于正常的B细胞
和脾细胞都没有影响。
42
(IC50 < 10 µM). Inhibits NF-κB -mediated gene transcription in cells by blocking the phosphorylation and degradation of IκBα.
IKK Inhibitor III, BMS-345541 (4-(2’-Aminoethyl)amino-1,8-dimethylimidazo[1,2-a]
quinoxaline)
A cell-permeable, potent, selective, and allosteric site-binding inhibitor of IKK-2 (IC50 ~300 nM) that displays anti-in�ammatory properties. Exhibits ~10-fold greater selectivity for IKK-2 over IKK-1 (IC50 ~4.0 µM).
1 mg 401480
IKK Inhibitor VII A cell-permeable benzamido-pyrimidine compound that acts as a potent, selective, and ATP-competitive inhibitor of IKK (IC50 = 40 nM, 70 nM, and 200 nM for IKK-2, IKK complex, and IKK-1, respectively).
1 mg 401486
IKK Inhibitor X (N-(6-Chloro-9H-β-carbolin-8-yl)nicotinamide)
A cell-permeable β-carboline that acts as a potent, ATP-competitive, and reversible inhibitor of IKK (IC50 = 88 nM) with selectivity over 14 other commonly studied kinases (IC50 > 100 µM), including NF-κB inducing kinase.
5 mg 401489
IKK Inhibitor XII A cell-permeable amino-diarylbenzamide compound that acts as a potent ATP site-targeting inhibitor against IKK-1 and IKK-2 (pIC50 = 6.4 and 7.0, respectively).
5 mg 401491
IKK Inhibitor XIII, 17-AJB A cell-permeable, irreversible inhibitor against IKK-β activity (IC50 = 300 nM). 1 mg 401493
IKK-2 Inhibitor IV ([5-(p-Fluorophenyl)-2-ureido]thiophene-3-carboxamide,
TPCA-1)
A cell-permeable, reversible, ATP-competitive, and potent inhibitor of IKK-2 (IC50 = 18 nM) that exhibits selectivity over IKK-1, JNK, and p38 MAPK.
500 µg 401481
InSolution™ IKK-2 Inhibitor IV A 10 mM (500 µg/179 µL) solution of IKK-2 Inhibitor IV (Catalogue No. 401481) in DMSO.
500 µg 401484
PI3-Kinase/Akt Signaling磷脂酰肌醇3-激酶(PI3Ks)蛋白家族参与细胞增殖、分化、凋亡和葡萄糖转运等多种细胞功能的调节。在
PI3K家族中,研究最广泛的是能被细胞表面受体所激活的I型PI3K。PI3K激活的结果是在质膜上产生第二
信使PIP3, PIP3与细胞内含有PH结构域的信号蛋白Akt和PDK1(phosphoinositide-ependent kinase-1)结合, 促使
PDK1磷酸化Akt蛋白的Ser308导致Akt的活化。Akt还能通过PDK2(如整合素连接激酶ILK)对其Thr473的磷酸化
而被激活。活化的Akt通过磷酸化作用激活或抑制其下游靶蛋白Bad、Caspase9、NF-κB、GSK-3、FKHR、p21Cip1和p27 Kip1等,进而调节细胞的增殖、分化、凋亡以及迁移等。
近年来发现,IA型PI3K和其下游分子蛋白激酶B(PKB或Akt)所组成的信号通路与人类肿瘤的发生发展密切
相关。该通路调节肿瘤细胞的增殖和存活,其活性异常不仅能导致细胞恶性转化,而且与肿瘤细胞的迁
移、黏附、肿瘤血管生成以及细胞外基质的降解等相关,目前以PI3K-Akt信号通路关键分子为靶点的肿
瘤治疗策略正在发展中。
Description Target Catalogue No.
Akt Inhibitor IV Akt 124011
Akt Inhibitor VIII, Isozyme-Selective, Akti-1/2 Akt1, Akt2 124018
LY 294002 PI3-K 440202
LY 303511 Negative control for LY 294002 440203
PDK1/Akt/Flt Dual Pathway Inhibitor Akt, Flt, PDK1 521275
PI-103 DNA-PK, PI3-K, mTOR 528100
PI3-K γ Inhibitor PI3-K γ 528106
PI3-K γ Inhibitor II PI3-K γ 528108
PI3-K α Inhibitor IV PI3-K α + β 528111
PI3-K α Inhibitor VIII DNA-PK, PI3-K 528116
Rapamycin mTOR, p70S6 553210
Ro-31-8220 PKC, GSK-3 557520
Wortmannin PI3-K Irreversible 681675
InhibitorSelect™ PI3-K/Akt/mTOR Signaling Pathway Inhibitor Panel (Catalogue No. 124031)
IGF-1
IGF-1R
PKCα
Growth Factors
RTKs
JAK1
PI 3-K
• LY 294002 • PI-103 • PI 3-Kγ Inhibitor • PI 3-Kγ Inhibitor II • PI 3-Kα Inhibitor IV • PI 3-Kα Inhibitor VIII • Wortmannin
• PDK1-Akt-Flt Dual Pathway Inhibitor • Akt Inhibitor IV, Akt Inhibitor VIII
• PDK1-Akt-Flt Dual Pathway Inhibitor
• PI-103, PI 3-Kα Inhibitor VIII
• Ro-31-8220
JAK1GAB1 BCAP
MDM2
MDM2Nucleus
p53
HSP90CDC37
Cytokines AG
Cytokine Receptor BCRBCR
4EBP1
elF4E
PDK-1
Raf1
• PI-103 • Rapamycin
mTor
p70s6K
PP2A
DNA-PK
SYK
Caspase 9
Caspase Cascade
ERK Pathway
Translation
Protein Synthesis
p53 Degradation
GlycogenSynthesis
GSK-3
Akt
Phosphorylation Ubiquitin GTP Calbiochem® Inhibitors
*以上组分可按目录号单独购买43
ANTI-PHOSPHO-AKT1 (Y326); 50 UG
ANTI-PHOSPHO-AKT (THR308), CLONE 50-1C-25; 100 UL
ANTI-AKT/PKB, PH DOMAIN, CLONE SKB1, ALEXA FLUOR(R)
488 CONJUGATE; 50 UG
ANTI-AKT/PKB, PH DOMAIN, CLONE SKB1, ALEXA FLUOR(R)
555 CONJUGATE; 50 UG
ANTI-AKT/PKB, PH DOMAIN, CLONE SKB1, ALEXA FLUOR(R)
555 CONJUGATE; 50 UG
ANTI-AKT/PKB, PH DOMAIN, CLONE SKB1, 100 UG
ANTI-AKT3/PKBGAMMA, CLONE GMA104; 100 UG
ANTI-AKT/PKB, PH DOMAIN, CLONE SKB1; 100 UG
ANTI-AKT1/PKBALPHA, CLONE AW24; 100 UL
ANTI-AKT3/PKBGAMMA; 100 UL
ANTI-PHOSPHO-AKT (SER473), CLONE 6F5; 100 UG
ANTI-PHOSPHO-AKT1/PKBALPHA (SER473), CLONE SK703; 100 UL
ANTI-PHOSPHO-AKT1/PKBALPHA (THR308), CLONE NL50; 100 UL
ANTI-PHOSPHO-AKT1/PKBALPHA (SER473), CLONE 11E6; 100 UG
ANTI-AKT2/PKBBETA, CLONE AW114; 100 UL
ANTI-AKT2/PKBBETA, CLONE AW114; 100 UL
ANTI-AKT2/PKBBETA; 200 UG
ANTI-PHOSPHO-AKT (THR34); 100 UL
ANTI-PHOSPHO-AKT1/PKBALPHA (SER473), CLONE SK703; 100 UG
ANTI-PHOSPHO-AKT1/PKBALPHA (THR308), CLONE NL50; 100 UL
ANTI-AKT1/PKBALPHA, CLONE AW24; 100 UL
ANTI-PHOSPHO-AKT1 (TYR326); 100 UG
ANTI-PHOSPHO-AKT (THR308); 100 UL
ANTI-PHOSPHO-AKT (TYR450); 100 UG
ANTI-PI3 KINASE, P110DELTA RABBIT MONOCLONAL; 100 UL
ANTI-PI3 KINASE ALPHA, CLONE 2B12.1; 100 UG
ANTI-PI3 KINASE P85 ANTIBODY, N-SH2 DOMAIN , CAT. NO. ABS233
ANTI-PI3 KINASE, P110ALPHA; 200 UG
ANTI-PI3 KINASE, P110GAMMA; 100 UL
ANTI-PI3 KINASE, P110ALPHA RABBIT MONOCLONAL; 100 UL
ANTI-PI3K TYPE 3, CLONE EPR5301, RABBIT MONOCLONAL
ANTI-PI3 KINASE, P85ALPHA RABBIT MONOCLONAL; 100 UL
ANTI-PI3 KINASE, P85, N-SH2, CLONE UB93-3; 200 UL
ANTI-PI3 KINASE ANTIBODY, P85 , CAT. NO. ABS234
ANTI-PI3 KINASE, P85; 125 UL
ANTI-PI3 KINASE, P110DELTA, CLONE AW103; 200 UG
ANTI-PI3 KINASE, P110BETA, RABBIT MONOCLONAL; 100 UL
ANTI-PI3 KINASE, P110ALPHA; 100 UG
ANTI-PI3 KINASE, P110BETA;100 UL
ANTI-PI3 KINASE BETA, P110B, CLONE 8C3.2; 100 UG
ANTI-PI3 KINASE, P110GAMMA, CLONE 17D7.2; 100 UG
ANTI-PI3-KINASE TYPE 3, CLONE 17D1.1; 100 UG
ANTI-PI3 KINASE-C2-ALPHA, CLONE 9H4.2; 100 UG
ANTI-PI3 KINASE-C2BETA; 100 UG
ANTI-PI3K TYPE 3
ANTI-PHOSPHO-RAF-1 (SER338); 100 UG
ANTI-RAF-1, CLONE AM223; 100 UL
ANTI-PHOSPHO-RAF-1 (TYR340/TYR341); 100 UL
ANTI-PHOSPHO-RAF-1 (SER338); 200 UG
大鼠;小鼠;鸡;马;犬;牛
人;小鼠;兔;牛;鸡
人;小鼠;大鼠
人;小鼠;大鼠
人;小鼠;大鼠
人;小鼠;大鼠
人
人;小鼠;大鼠
人;小鼠;大鼠
人
人;小鼠;大鼠;脊椎动物
人;小鼠
人;小鼠
小鼠;大鼠;人;犬
人;小鼠;大鼠
人;大鼠
人;小鼠;大鼠
人
人;小鼠
人;小鼠;牛;鸡;大鼠
人;小鼠;大鼠
人;小鼠;大鼠;犬;非洲爪蟾
人;小鼠;大鼠;牛;非洲爪蟾;犬
小鼠;大鼠;人;牛;鸡;马
人
小鼠
人;大鼠
人;脊椎动物
人
人
人;小鼠;大鼠
人;小鼠;大鼠
人;小鼠;大鼠
人;大鼠
人;小鼠;猴子;大鼠
人
人
人;大鼠
人;猴子;犬;小鼠;大鼠
大鼠;人;小鼠
人;小鼠;大鼠
人;小鼠;大鼠
小鼠;大鼠;人
人;黑猩猩;恒河猴
人;小鼠
小鼠
人;小鼠;大鼠
人
小鼠
WB
WB;IF;ICC;ELISA
FC;ICC;IF;WB
FC;ICC;IF;WB
FC;ICC;IF;WB
FC;ICC;IF;WB
IP;WB
FC;ICC;IP;WB
IP;WB
IP;WB
ELISA;WB;FC;IF;IHC
WB
WB
ELISA;WB
WB;IP
IP;WB
IP;WB
WB
WB
WB
WB;IP
WB
IHC(P)
WB;Enzyme Assay
IF;IP;WB
WB
WB;IP
IP
FC;IP;WB
IF;IP;WB
WB;IHC
FC;ICC;IF;IHC(P);IP;WB
WB;IP
WB;IP
IP;WB
WB
FC;IP;WB
WB;IP;IHC;ELISA
WB;IP;ICC;IHC;ELISA
WB;ICC
WB;ICC
WB;ICC
WB;ICC
WB;IHC
ICC
WB
IP;WB
WB
WB
50UG
100UL
50UG
50UG
50UG
100UG
100UG
100UG
100UL
100UL
100UG
100UL
100UL
100UG
100UL
100UL
200UG
100UL
100UG
100UL
100UL
100UG
100UL
100UG
100UL
100UG
100UG
200UG
100UL
100UL
100uL
100UL
200UL
200UL
125UL
200UG
100UL
100UG
100UL
100UG
100UG
100UG
100UG
100UG
100ug
100UG
100UL
100UL
200UG
09-288
05-802R
16-293
16-294
05-780
05-591
05-796
07-383
05-1003
04-736
05-802
05-669
04-771
05-771
07-372
07-789
05-736
04-802
04-796
AB9927
07-1398
07-1643
04-401
MABS76
ABS233
07-658
04-402
04-399
MABN1114
04-403
05-217
ABS234
06-195
05-703
04-400
09-481
09-482
05-1558
05-1559
05-1563
05-1560
07-2170
07-2186
05-534
04-739
07-815
04-412
05-538
MABS14
PI3-K/Akt 信号通路研究抗体产品描述 反应种属 应用 体积 货号
参考文献:
Phosphatidylinositol 3-kinase/AKT Signalling Pathway Components in Human Breast Cancer: Clinicopathological Correlations. Anticancer Res. 2007 Jul-Aug;27(4A):1777-82.
Tyrosine phosphorylation of Disabled-1 is essential for Reelin-stimulated activation of Akt and Src family kinases. doi:10.1016/S0169-328X(03)00295-X
Impaired PTPN13 phosphatase activity in spontaneous or HPV-induced squamous cell carcinomas potentiates oncogene signaling through the MAP kinase pathway. doi:10.1038/onc.2009.251
Catalase expression in MCF-7 breast cancer cells is mainly controlled by PI3K/Akt/mTor signaling pathway. doi:10.1016/j.bcp.2014.02.025
Ketamine Affects the Neurogenesis of Rat Fetal Neural Stem Progenitor Cells via the PI3K/Akt-p27 Signaling Pathway. DOI: 10.1002/bdrb.21119
44
PI3-K/Akt 信号通路研究抗体产品描述 反应种属 应用 体积 货号
ANTI-PHOSPHO-AKT1 (Y326); 50 UG
ANTI-PHOSPHO-AKT (THR308), CLONE 50-1C-25; 100 UL
ANTI-AKT/PKB, PH DOMAIN, CLONE SKB1, ALEXA FLUOR(R) 488 CONJUGATE; 50 UG
ANTI-AKT/PKB, PH DOMAIN, CLONE SKB1, ALEXA FLUOR(R) 555 CONJUGATE; 50 UG
ANTI-AKT3/PKBGAMMA, CLONE GMA104; 100 UG
ANTI-AKT/PKB, PH DOMAIN, CLONE SKB1; 100 UG
ANTI-AKT1/PKBALPHA, CLONE AW24; 100 UL
ANTI-AKT3/PKBGAMMA; 100 UL
ANTI-PHOSPHO-AKT (SER473), CLONE 6F5; 100 UG
ANTI-PHOSPHO-AKT1/PKBALPHA (SER473), CLONE SK703; 100 UL
ANTI-PHOSPHO-AKT1/PKBALPHA (THR308), CLONE NL50; 100 UL
ANTI-PHOSPHO-AKT1/PKBALPHA (SER473), CLONE 11E6; 100 UG
ANTI-AKT2/PKBBETA, CLONE AW114; 100 UL
ANTI-AKT2/PKBBETA, CLONE AW114; 100 UL
ANTI-AKT2/PKBBETA; 200 UG
ANTI-PHOSPHO-AKT (THR34); 100 UL
ANTI-PHOSPHO-AKT1/PKBALPHA (SER473), CLONE SK703; 100 UG
ANTI-PHOSPHO-AKT1/PKBALPHA (THR308), CLONE NL50; 100 UL
ANTI-AKT1/PKBALPHA, CLONE AW24; 100 UL
ANTI-PHOSPHO-AKT1 (TYR326); 100 UG
ANTI-PHOSPHO-AKT (THR308); 100 UL
ANTI-PHOSPHO-AKT (TYR450); 100 UG
ANTI-PI3 KINASE, P110DELTA RABBIT MONOCLONAL; 100 UL
ANTI-PI3 KINASE ALPHA, CLONE 2B12.1; 100 UG
ANTI-PI3 KINASE P85 ANTIBODY, N-SH2 DOMAIN , CAT. NO. ABS233
ANTI-PI3 KINASE, P110ALPHA; 200 UG
ANTI-PI3 KINASE, P110GAMMA; 100 UL
ANTI-PI3 KINASE, P110ALPHA RABBIT MONOCLONAL; 100 UL
ANTI-PI3K TYPE 3, CLONE EPR5301, RABBIT MONOCLONAL
ANTI-PI3 KINASE, P85ALPHA RABBIT MONOCLONAL; 100 UL
ANTI-PI3 KINASE, P85, N-SH2, CLONE UB93-3; 200 UL
ANTI-PI3 KINASE ANTIBODY, P85 , CAT. NO. ABS234
ANTI-PI3 KINASE, P85; 125 UL
ANTI-PI3 KINASE, P110DELTA, CLONE AW103; 200 UG
ANTI-PI3 KINASE, P110BETA, RABBIT MONOCLONAL; 100 UL
ANTI-PI3 KINASE, P110ALPHA; 100 UG
ANTI-PI3 KINASE, P110BETA;100 UL
ANTI-PI3 KINASE BETA, P110B, CLONE 8C3.2; 100 UG
ANTI-PI3 KINASE, P110GAMMA, CLONE 17D7.2; 100 UG
ANTI-PI3-KINASE TYPE 3, CLONE 17D1.1; 100 UG
ANTI-PI3 KINASE-C2-ALPHA, CLONE 9H4.2; 100 UG
ANTI-PI3 KINASE-C2BETA; 100 UG
ANTI-PI3K TYPE 3
ANTI-PHOSPHO-RAF-1 (SER338); 100 UG
ANTI-RAF-1, CLONE AM223; 100 UL
ANTI-PHOSPHO-RAF-1 (TYR340/TYR341); 100 UL
ANTI-RAF-1, RABBIT MONOCLONAL; 100 UL
ANTI-PHOSPHO-RAF-1 (SER338); 200 UG
ANTImTOR/FRAP (AB-2) mouse MAB (22C2)
ANTImTOR, CLONE F11; 100 UG
ANTImTOR, RABBIT MAB; 100 UL
ANTI-PHOSPHOmTOR (SER2448)
ANTI-PHOSPHOmTOR (SER2481); 100 UL
ANTI-PHOSPHOmTOR (THR2446); 100 UL
ANTImTOR, A.A.1223-1290; 100 UG
ANTImTOR, CLONE 21A12.2; 100 UG
ANTImTOR, CLONE 2ID8.2; 100 UG
ANTImTOR/FRAP, CLONE EPR390(N), RABBIT MONOCLONAL
ANTImTOR/FRAP, CLONE 22C2
ANTI-PHOSPHOmTOR (SER2159); 100 UL
ANTI-PHOSPHOmTOR (THR2164)
ANTI-P70 S6 KINASE; 200 UG
ANTI-PHOSPHO-P70 S6 KINASE (THR389)
ANTI-P70 S6 KINASE (S6K); 200 UG
ANTI-PHOSPHO-P70 S6 KINASE (THR421/SER424), RABBIT
MONOCLONAL; 100 UL
ANTI-PHOSPHO-P70 S6 KINASE (THR389)
ANTI-P70 S6 KINASE, RABBIT MONOCLONAL; 100 UL
ANTI-PHOSPHO-P70S6K (THR470)
ANTI-P70 S6 KINASE, CLONE 20-10C-6; 100 UL
大鼠;小鼠;鸡;马;犬;牛
人;小鼠;兔;牛;鸡
人;小鼠;大鼠
人;小鼠;大鼠
人
人;小鼠;大鼠
人;小鼠;大鼠
人
人;小鼠;大鼠;脊椎动物
人;小鼠
人;小鼠
小鼠;大鼠;人;犬
人;小鼠;大鼠
人;大鼠
人;小鼠;大鼠
人
人;小鼠
人;小鼠;牛;鸡;大鼠
人;小鼠;大鼠
人;小鼠;大鼠;犬;非洲爪蟾
人;小鼠;大鼠;牛;非洲爪蟾;犬
小鼠;大鼠;人;牛;鸡;马
人
小鼠
人;大鼠
人;脊椎动物
人
人
人;小鼠;大鼠
人;小鼠;大鼠
人;小鼠;大鼠
人;大鼠
人;小鼠;猴子;大鼠
人
人
人;大鼠
人;猴子;犬;小鼠;大鼠
大鼠;人;小鼠
人;小鼠;大鼠
人;小鼠;大鼠
小鼠;大鼠;人
人;黑猩猩;恒河猴
人;小鼠
小鼠
人;小鼠;大鼠
人
人;小鼠;大鼠
小鼠
人
人;大鼠
人;小鼠;大鼠
人
人;大鼠
人;小鼠;大鼠
人;大鼠
人
人;小鼠
人
人
人;小鼠;大鼠;羊;斑马鱼
人
人;小鼠;大鼠
人;小鼠
人;小鼠;大鼠
人
人
人;小鼠;大鼠
人
人;小鼠;大鼠
WB
WB;IF;ICC;ELISA
FC;ICC;IF;WB
FC;ICC;IF;WB
IP;WB
FC;ICC;IP;WB
IP;WB
IP;WB
ELISA;WB;FC;IF;IHC
WB
WB
ELISA;WB
WB;IP
IP;WB
IP;WB
WB
WB
WB
WB;IP
WB
IHC(P)
WB;Enzyme Assay
IF;IP;WB
WB
WB;IP
IP
FC;IP;WB
IF;IP;WB
WB;IHC
FC;ICC;IF;IHC(P);IP;WB
WB;IP
WB;IP
IP;WB
WB
FC;IP;WB
WB;IP;IHC;ELISA
WB;IP;ICC;IHC;ELISA
WB;ICC
WB;ICC
WB;ICC
WB;ICC
WB;IHC
ICC
WB
IP;WB
WB
IF;IHC(P);IP;WB
WB
WB;IP
WB;IP
FC;ICC;IHC(P);IP;WB
WB
WB
WB
WB;IP;IHC(P)
WB;ICC
WB;ICC
WB
WB;IP
WB;Peptide Inhibition
Assay;Dot Blot
Dot Blot;WB
IP;WB
WB;ICC
WB
IP;WB
WB;Peptide Inhibition Assay
FC;IHC(P);IP;WB
WB
WB;IP;ICC
50UG
100UL
50UG
50UG
100UG
100UG
100UL
100UL
100UG
100UL
100UL
100UG
100UL
100UL
200UG
100UL
100UG
100UL
100UL
100UG
100UL
100UG
100UL
100UG
100UG
200UG
100UL
100UL
100uL
100UL
200UL
200UL
125UL
200UG
100UL
100UG
100UL
100UG
100UG
100UG
100UG
100UG
100ug
100UG
100UL
100UL
100UL
200UG
100ug
100UG
100UL
100UG
100UL
100UL
100UG
100UG
100UG
100uL
100UG
100uL
100UL
200UG
100ug
200UG
100UL
100UG
100UL
200UL
100UL
09-288
05-802R
16-293
16-294
05-780
05-591
05-796
07-383
05-1003
04-736
05-802
05-669
04-771
05-771
07-372
07-789
05-736
04-802
04-796
AB9927
07-1398
07-1643
04-401
MABS76
ABS233
07-658
04-402
04-399
MABN1114
04-403
05-217
ABS234
06-195
05-703
04-400
09-481
09-482
05-1558
05-1559
05-1563
05-1560
07-2170
07-2186
05-534
04-739
07-815
04-412
05-538
OP97-100UG
MABS14
04-385
09-213
09-343
09-345
07-1415
05-1564
05-1592
MABS799
MABS196
ABS79
ABS88
07-402
MABS82
06-926
04-393
07-018-I
04-391
ABS431
05-781R
参考文献:
Epigallocatechin gallate (EGCG), a major component of green tea, is a dual phosphoinositide-3-kinase/mTOR inhibitor. doi:10.1016/j.bbrc.2011.02.010
Inhibition of tumor cell growth, proliferation and migration by X-387, a novel active-site inhibitor of mTOR. doi:10.1016/j.bcp.2012.01.019
Immunohistochemical assessment of PTEN in vulvar cancer: Best practices for tissue staining, evaluation, and clinical association. doi:10.1016/j.ymeth.2014.12.017
45
AB5605AB5830MAB3560
AB9890
07-520
MAB5310AB3282
MABN527ABS97MABC71307-685
MABN457
06-984
ABC391AB5380AB5382
AB16301AB553205-233
16-226
16-207
16-310
07-61007-35707-56107-562AB5484MAB5328
ABC245MABC684
AB10346AB5482AB9328
Anti-4-Hydroxynonenal AntibodyAnti-8-Hydroxydeoxyguanosine AntibodyAnti-8-Oxoguanine Antibody, clone 483.15
Anti-AGE (Advanced Glycated End-products) AntibodyAnti-eNOS/NOS III Antibody, CT
Anti-Glutathione Antibody, clone D8Anti-Glutathione-S-Transferase Antibody, S. japonicum formAnti-iNOS/NOS II, clone 13F5.1 AntibodyAnti-KEAP1 AntibodyAnti-KEAP1, clone 7G4B10 AntibodyAnti-Lactotransferrin Antibody
Anti-Mitochondrial dicarboxylate carrier Antibody, clone 1F5.1Anti-Mn-SOD Antibody
Anti-NDUFB7 AntibodyAnti-Nitric Oxide Synthase I AntibodyAnti-Nitric Oxide Synthase II Antibody
Anti-Nitric Oxide Synthase III AntibodyAnti-Nitrotyrosine AntibodyAnti-Nitrotyrosine Antibody, clone 1A6
Anti-Nitrotyrosine Antibody, clone 1A6, Alexa Fluor® 488 conjugateAnti-Nitrotyrosine Antibody, clone 1A6, HRP conjugateAnti-Nitrotyrosine Magnetic Bead Conjugate AntibodyAnti-Peroxiredoxin 2 AntibodyAnti-phospho-eNOS/NOS III (Ser116) AntibodyAnti-phospho-eNOS/NOS III (Ser617) AntibodyAnti-phospho-eNOS/NOS III (Ser635) AntibodyAnti-RAGE AntibodyAnti-RAGE Antibody, clone DD/A11 or A11
Anti-REDD1 AntibodyAnti-SOD1 Antibody, clone 6F5
Anti-SOD2 AntibodyAnti-Superoxide Dismutase 1 AntibodyAnti-Thioredoxin 1 Antibody
ELISA;Western BlottingELISA;Immunohistochemistry (Paraffin)ELISA;Immunocytochemistry;Chromatin Immunoprecipitation (ChIP)ELISA;Western Blotting
Western Blotting
Western BlottingELISA;Western Blotting
Western Blotting;ImmunohistochemistryWestern Blotting;ImmunocytochemistryImmunohistochemistry;Flow Cytometry;ELISAImmunoprecipitation;Western Blotting;Immunocytochemistry;Immunohistochemistry;Immunohistochemistry (Paraffin)Western Blotting;Immunohistochemistry
Immunohistochemistry;Immunohistochemistry (Paraffin);Western BlottingWestern Blotting;ImmunohistochemistryImmunohistochemistry;Western BlottingImmunocytochemistry;Immunohistochemistry;Western BlottingWestern Blotting;ImmunocytochemistryELISA;ImmunohistochemistryImmunocytochemistry;Immunohistochemistry;Immunoprecipitation;Western BlottingFlow Cytometry;Western Blotting
ELISA;Western Blotting
Immunoprecipitation
Western Blotting;ImmunocytochemistryWestern BlottingWestern BlottingWestern BlottingELISA;Immunohistochemistry (Paraffin)ELISA;Immunocytochemistry;Immunohistochemistry;Western BlottingWestern Blotting;Immunohistochemistry (Paraffin)Western Blotting;Immunocytochemistry;Flow CytometryWestern BlottingWestern BlottingWestern Blotting
HumanHumanHuman;Rat;Mouse;Monkey
Human
Human;Rat;Bovine;Mouse;Canine;Guinea Pig;PigEukaryoteVertebrates;Schistosoma japonicum
HumanHuman;Mouse;RatHumanHuman;Mouse;Rat;Hamster;Monkey
Mouse;Rat
Human;Rat;Mouse;Bovine
Human;MouseHuman;Mouse;Monkey;Rat;SheepMouse;Rat
HumanAllVertebrates
Vertebrates
All
All
HumanBovine;HumanBovineBovineHumanBovine;Human;Mouse
Human;Mouse;RatHuman;Mouse
Human;Rat;MouseMouse;RatMouse;Rat
200 µL200 µL100 µL
200 µL
200 µg
100 µg500 µg
100 µL100 µg100 µg100 µL
100 µg
200 µg
150 µL50 µL50 µL
100 µg100 µL100 µg
100 µg
50 µL
200 µL
50 µL100 µL200 µL200 µL200 µL100 µg
100 µg100 µL
50 µL50 µL100 µL
Akt 抑制剂Product Cat. No. Comments Size Price
Akt Inhibitor 124005 A PI analog, prevents PIP3 formation and binding to Akt. Inhibits Akt (IC50 = 5.0 M) and PI 3-K (IC50 = 83 M)
1 mg
Akt Inhibitor II (SH-5) 124008 A PI analog, prevents PIP3 formation and binding to Akt. Metabolically more stable than Akt Inhibitor (Cat. No. 124005).
1 mg
Akt Inhibitor III (SH-6) 124009 A PI analog, prevents PIP3 formation and binding to Akt. Metabolically more stable than Akt Inhibitor (Cat. No. 124005).
1 mg
Akt Inhibitor IV 124011 A cell-permeable, ATP-competitive inhibitor of a kinase upstream of Akt, but downstream of PI 3-K.
1 mg5 mg
InSolution™Akt Inhibitor IV
124015 A 10 mM solution of Akt Inhibitor IV (Cat. No. 124011) in DMSO. 1 mg
Akt Inhibitor V, Triciribine (API-2, NSC 154020, TCN)
124012 A cell-permeable inhibitor of Akt. Blocks cellular phosphorylation/activation of Akt1/2/3 by targeting an Akt effector molecule other than PI 3-K or PDK1. Has shown ef�cacy in vivo.
1 mg
Akt Inhibitor VI, Akt-in 124013 A15-mer peptide that directly binds Akt PH domain (kd=18 M), preventing PI binding. 2 mg
Akt Inhibitor VII, TAT-Akt-in 124014 A cell-permeable version of Cat. No. 124013 that directly binds Akt PH domain, preventing PI binding. Has shown ef�cacy in vivo.
2 mg
Akt Inhibitor VIII, Isozyme-Selective, Akt-1/2
124018 A cell-permeable inhibitor of Akt. Inhibition is PH domain dependent. Shown to block basal and stimulated phosphorylation/activation of Akt1/Akt2 in cultured cells in vitro and in mice in vivo (IC50 = 58 nM, 210 nM, and 2.12 M for Akt1, Akt2, and Akt3, respectively, in vitro kinase assays).
1 mg
InSolution™ Akt Inhibitor VIII, Isozyme-Selective, Akti-1/2
124017 A 10 mM (1 mg/181 µl) solution of Akt Inhibitor VIII, Isozyme-Selective, Akti-1/2 (Cat. No. 124018) in DMSO.
1 mg
Akt Inhibitor IX, API-59CJ-OMe
124019 A cell-permeable ellipticine compound that potently and selectively inhibits cell growth and induces apoptosis in human endometrial cancer cells with elevated Akt levels. Exhibits minimal effect on cells with low Akt activity.
5 mg
Akt Inhibitor X 124020 A cell-permeable, selective inhibitor of the phosphorylation of Akt and its in vitro kinase activity (complete inhibition < 5 M) with minimal effect on PI 3-K, PDK1, or SGK1. Unlike Akti1/2 (Cat. No. 124018), the mode of inhibition is not PH domain-dependent.
5 mg
Akt Inhibitor XI 124028 A cell-permeable copper complex (Cu2+/Cu+ redox couple in the range of +0.28 to +0.35 V) that interacts with both the PH and the kinase domains of Akt and potently inhibits its kinase activity (IC50 = 100 nM). Inhibits tumor growth both in cultured cells in vitro (IC50 ranges from 10 to 34 M) and in mice in vitro (25 mg/kg, iv) without any apparent adverse effect to the animals.
5 mg
(-)-Deguelin, Mundulea serica
252740 A cell-permeable, potent inhibitor of mitochondrial bioenergetics (IC50 = 6.9 nM for NADH: ubiquinone oxidoreductase activity in bovine heart ETP). Promotes mitochondrial permeability transition. Selectively blocks Akt activation with minimal effects on MAPK signaling. Also shown to activate AMPK activity and inhibit COX-2 expression.
5 mg
Naltrindole, Hydrochloride 476880 A cell-permeable inhibitor of Akt signaling. Decreases phosphorylation level of PDK1, Akt, FKHR/AFX, GSK-3 , and inhibits Akt-dependent cell growth in small cell lung cancer (SCLC) cell line (IC50 = 25, 40, and 55 M in NCI-H69, NCI-H345, and NCI-H510, respectively).
5 mg
PDK1/Akt/Flt Dual Pathway Inhibitor
521275 A cell-permeable inhibitor of PDK1 and Akt in in vitro kinase assays. Blocks phosphorylation of Akt at both Ser473 and Thr308.
5 mg
mTOR 抑制剂Product Cat. No. Comments Size Price
Rapamycin 553210 Anti-fungal and immunosuppressant. Inhibits mTOR by binding to FK506-binding protein-12. Selectively inhibits the phosphorylation and activation of p70 S6 kinase (IC50 = 50 pM). Prevents the translational activation of IGF-II. Shown to inhibit later signaling events such as p110Rb phosphorylation, p34cdk1 kinase activation, and cyclin A synthesis. Reported to induce apoptosis in a murine B cell line, to inhibit lympho-kine-induced cell proliferation at the G1 phase, and to irreversibly arrest Saccharomyces cerevisiae G1 phase.
100 g 1 mg
InSolution™ Rapamycin 553211 Supplied as a 5 mM (500 g/109 l) solution of Rapamycin (Cat. No. 553210) in DMSO. 500 g
PI-103 528100 A cell-permeable pyridinylfuranopyrimidine compound that acts as a potent and ATP-competitive inhibitor of DNA-PK, PI 3-K, and mTOR (IC50 = 2, 8, 88, 48, 150, 26, 20, and 83 nM for DNA-PK, p110 , p110 , p110 , p110 , PI 3-KC2 , mTORC1, and mTORC2, respectively). It inhibits ATR and ATM only at much higher concentrations (IC50 = 850 and 920 nM, respectively) and exhibits little activity towards a panel of more than 40 other kinases even at concentrations as high as 10 M. Shown to effectively block PI 3-K/Akt signaling and cell proliferation in glioma cell lines both in vitro and in vivo.
1 mg 5 mg
46
PI 3-Kinase 抑制剂Product Cat. No. Comments Size Price
ET-18-OCH3 341207 A selective cell-permeable inhibitor of phosphatidylinositol-speci�c PLC (IC50 = 15 mM) but does not inhibit phosphatidylcholine-speci�c PLC or PLD.
5 mg
DNA-PK Inhibitor III 260962 A cell-permeable, potent, selective, ATP-competitive inhibitor of DNA-PK (IC50 = 120 nM) and PI 3-Kinase catalytic subunit p110b (IC50 = 135 nM). Inhibits PI 3K p110a , p110g, and p110d only at much higher concentrations (IC50 = 1.4, 0.88, and 1.0 mM, respectively).
1 mg
LY 294002 440202 A cell-permeable, potent, and speci�c inhibitor of PI 3-K (IC50 = 1.4 mM). Acts on the ATP-binding site of the enzyme.
5 mg
InSolution™ LY 294002 440204 Supplied as a 10 mM (1 mg/325 ml) solution of LY 294002 (Cat. No. 440202) in DMSO. 1 mg
LY 303511 440203 A cell-permeable negative control for the PI 3-kinase inhibitor, LY 294002 (Cat. No. 440202). Contains a single atom substitution in the morpholine ring compared to LY 294002. Does not affect PI 3-kinase activity even at concentrations ≥100 mM.
1 mg
PI-103 528100 A cell-permeable pyridinylfuranopyrimidine compound that displays antitumor properties in a mouse model of human glioma. Acts as a potent and ATP-competitive inhibitor of DNA-PK, PI 3-K and mTOR (IC50 in nM = 2, 8, 88, 48, 150, 26, 20 and 83 for DNA-PK, p110a , p110b, p110d, p110g, PI 3-KC2b, mTORC1 and mTORC2, respectively) and exhibits little activity towards a panel of more than 40 other kinases, even at concentations as high as 10 mM. Reported to block PIP3 production, insulin signaling and Akt-phosphorylation, and to inhibit cell proliferation and induce cell-death.
1 mg 5 mg
PI 3-Kg Inhibitor 528106 A cell-permeable thiazolidinedione compound that acts as a potent, selective, and ATP-competitive inhibitor of PI 3-Kg (Ki = 7.8 nM; IC50 = 8 nM, 60 nM, 270 nM, 300 nM for p110-g, a , b and d-isoforms, respectively). Does not affect the activity of a wide panel of kinases, receptors, enzymes, and ion channels even at concentrations as high as 1 mM.
5 mg
PI 3-Kg Inhibitor II 528108 A cell-permeable, potent and ATP-competitive inhibitor of PI 3-Kg (Ki = 180 nM; IC50 = 250 nM). Exhibits great selectivity over PI 3-Ka (IC50 = 4.5 mM), PI 3-Kb and d (IC50 >20 mM).
5 mg
PI 3-Kg/CKII Inhibitor 528112 A cell-permeable, potent and ATP-competitive dual speci�c inhibitor of PI 3-Kg;/CKII (IC50 = 20 nM).
5 mg
Quercetin, Dihydrate 551600 An inhibitor of PI 3-kinase (IC50 = 3.8 mM) and phospholipase A2 (IC50 = 2 mM). 100 mg
Wortmannin 681675 A potent, selective, and irreversible inhibitor of PI 3-K (IC50 = 5 nM). Blocks the catalytic activity of PI 3-kinase without affecting the upstream signaling events.
1 mg
47
TGF-β Signaling转化生长因子-β(TGF-β)以及与其关系密切的骨形成蛋白(BMPs)参与调节分化、衰老、免疫反应、愈伤、凋亡等等重要生命过
程。TGF-β超家族包含接近30个生长和分化因子,其中有TGF-βs,activin,inhibins和BMPs。下游的跨膜TGF-β受体是多个SMAD
蛋白,这些蛋白是TGF-β超家族信号传递的重要调控分子,并在不同层面上受多种多样精确的调控。
MM 匹配抗体名MM匹配抗体编号 Key Applications Species Reactivity Qty/Pk
抗体
ABC345
MABC525
MAB4429
MAB4350
MABT117
05-1424
CBL1358
ABD7107-049MABE902AB4227
Anti-BAG-1 Antibody
Anti-BAG-1 Antibody, clone 3.10G3E2Anti-BMP-7 (Pro-peptide) Antibody, clone 2Anti-BMP-7 Antibody, clone 2A10Anti-Endoglin (CD105) Antibody, clone 44G4Anti-Endoglin Antibody, clone P3D1
Anti-Endoglin Antibody, Extracellular, clone MJ7/18Anti-FOXC1 AntibodyAnti-Nedd4 Antibody, WW2 domainAnti-Pax-2 Antibody, clone 4C2.2 Anti-Pax5 Antibody
Western Blotting;Immunohistochemistry;ImmunofluorescenceWestern Blotting;Immunoprecipitation;Immunohistochemistry;ELISAWestern Blotting;Immunohistochemistry (Paraffin);ELISA;ImmunocytochemistryWestern Blotting;ImmunohistochemistryFlow Cytometry;Immunohistochemistry;ImmunoprecipitationWestern Blotting;Immunohistochemistry;Immunocytochemistry;Immunoprecipitation;Flow Cytometry;ELISAFlow Cytometry;Immunoprecipitation;Western Blotting;ImmunohistochemistryWestern Blotting;ImmunocytochemistryImmunohistochemistry;Western BlottingWestern BlottingWestern Blotting
Human
Human
Human
Human;Mouse
Human
Human;Mouse;Rat
Mouse
Human;MouseHuman;Mouse;RatHumanHuman;Mouse
100 µg
100 µL
100 µg
100 µg
100 µL
100 µg
500 µg
100 µg200 µL200 µL200 µg
48
MM 匹配抗体名MM匹配抗体编号 Key Applications Species Reactivity Qty/Pk
抗体
05-1573
MABN711MABE1041
ABS52
FCMAB108A4
05-857
05-1478
05-1459
04-953
AB3849
06-920MABT521
ABE21ABD104
ABC330ABD133ABN476MABF347
MABF345
MABS477
MABF348
MABF346
06-227
ABD73MABD14609-726
Anti-Pax5 Antibody, clone 1H9
Anti-PAX5 Antibody, clone 7D3Anti-Pax-9 Antibody, clone 7C2 (P9C23) Anti-phospho-SMAD1 (Ser206) AntibodyAnti-phospho-SMC1 (Ser957) Antibody, clone 5D11G5 Alexa Fluor® 488Anti-phospho-Src family (Tyr416) Antibody, clone 2N8, rabbit monoclonalAnti-RUNX2 Antibody, clone AS110Anti-SMAD1 Antibody, clone AS22Anti-phospho-Smad2 (Ser465/467) Antibody, clone A5S, rabbit monoclonalAnti-Smad2 Antibody, phospho-specific (Ser465/467)
Anti-Smad3 AntibodyAnti-SMAD3, clone 5G11 AntibodyAnti-Smad4/DPC4 AntibodyAnti-SnoN Antibody
Anti-ACVR1 AntibodyAnti-ACVR1B AntibodyAnti-ACVR1C AntibodyAnti-LAP (TGF-β1) (human) Antibody, FITC, clone TW4-2F8Anti-LAP (TGF-β1) (mouse) Antibody, APC, clone TW7-16B4Anti-LAP (TGF-β1) Antibody, clone TW4-6H10Anti-TGF-β1 (human) Antibody, PE, clone TW4-2F8Anti-TGF-β1 (mouse) Antibody, PE, clone TW7-16B4Anti-TGFβR Type II Antibody, NT
Anti-Lefty AntibodyAnti-Lefty Antibody, clone 1G9.1Anti-PAI-1 Antibody
Western Blotting;Immunohistochemistry;ImmunoprecipitationWestern BlottingWestern Blotting;Immunohistochemistry
Western Blotting
Western Blotting;Flow Cytometry
Western Blotting
Western Blotting;Immunocytochemistry
Western Blotting;Immunofluorescence
Western Blotting
Immunocytochemistry;Immunofluorescence;Immunohistochemistry (Paraffin);Western BlottingWestern BlottingWestern Blotting;Immunocytochemistry
Western Blotting;ImmunocytochemistryWestern Blotting;Immunocytochemistry;ImmunohistochemistryImmunohistochemistryWestern BlottingWestern BlottingFlow Cytometry
Flow Cytometry;Western Blotting;Immunoprecipitation;NeutralizingFlow Cytometry
Flow Cytometry
Flow Cytometry;Western Blotting;Immunoprecipitation;NeutralizingImmunohistochemistry;Immunoprecipitation;Western BlottingWestern Blotting;ImmunocytochemistryWestern BlottingWestern Blotting
Mouse;Human
HumanMouse;Human
Human
Human;Xenopus;Bovine
Mouse;Vertebrates
Human
Human;Mouse
Canine;Human;Mouse;Zebrafish
Human;Mouse;Rat
Human;Mouse;RatHuman;Mouse
Human;Mouse;Rat;ChimpanzeeHuman
HumanHumanHumanHuman
Mouse
Human
Human
Mouse
Human;Mouse;Rat
Mouse;RatRat;MouseHuman
100 µg
100 µL100 µg
100 µg
100 tests
100 µL
100 µg
200 µg
100 µL
100 µL
400 µg100 µL
100 µg100 µg
100 µg100 µg100 µg100 tests
100 µg
100 µg
100 tests
100 µg
400 µg
100 µg100 µg100 µg
49
InhibitorSelect™TGF-βR and BMPR Signaling Pathway Inhibitor Panel,目录号616466
组分 靶标 包装量 目录号
Akt Inhibitor XIII,
Isozyme-Selective,Akti2-1/2
AMPK Inhibitor,Compound C
ET-18-OCH3
IRAK-1/4 Inhibitor
JAK Inhibitor Ⅰ
MG-132
(5Z)-7-Oxozeaenol,
Curvularia sp.
p21-Activated Kinase
Inhibitor Ⅲ,IPA-3
PD 169316
Akt1/2
BMPRI;AIk2/3/6
PI-PLC
IRAK-1& IRAK-4
JAK1/2/3,Tyk2
Proteasome
TAK1
PAK1/2/3
MAPK Smad
2mg
1mg
5mg
5mg
500μg
5mg
1mg
5mg
1mg
124030
171260
341207
407601
420099
474790
499610
506106
513030
组分 靶标 包装量 目录号
PI-103
Rac1 Inhibitor
Ro-31-8220
Smad3 Inhibitor,SIS3
STAT3 Inhibitor Ⅲ,WP1066
TGF-β RI Kinase Inhibitor Ⅱ
TGF-β RI Kinase Inhibitor Ⅵ,
SB431542
U0126
Y-27632
DMSO-15ML
DNA-PK,PI3-K,mTOR
Rac1
PKC,GSK-3,SIRT1/2
Smad3
JAK-STAT3 Sig Inh
TGF-β RI;AIk5
TGF-β RI;AIk4/5/7
MEK1/2
Rho Kinase
-
1mg
5mg
500μg
1mg
10mg
1mg
5mg
1mg
1mg
15ml
528100
553502
557520
566405
573097
616452
616461
662005
688000
KP31817
TGFβ - 6 Plex(Cat.No.48-614MAG)AB2
Analyte Total Phosphorylated
Akt/PKB
Erk/MAPK1/2
SMAD2
SMAD3
SMAD4
TGFβRII
√
√
√
√
√√ (Ser473)
(Thr185/Tyr187)
(Ser465/467)
(Ser423/425)
H
H,M,R
H,M,R
H,M,R
H,M,R
H
Milliplex Kit
*以上组分可按目录号单独购买
50
Toll-like Receptor (TLR) Signaling Toll-like Receptors (TLRs)是在多种免疫细胞中表达的跨膜蛋白。胞外的N末端区域识别特异的病原组分。目前已发现至少13个TLR家
族蛋白识别不同的病原物相关的分子结构(PAMPs),包括脂多糖,鞭毛蛋白,细菌CpG DNA,和病毒RNA、DNA。TLRs识别
PAMPs被认为是诱导免疫反应的关键步骤。TLRs也会和补体系统一起作用提供关键的先天防御。它们通过相互作用和拮抗反应来
加强先天免疫并调节过度的炎症反应。补体可以抑制TLR介导产生的IL-12。有报道显示巨噬细胞中C5aR的活化可以抑制TLR4诱导
的IL-12p35,IL-12/IL-23p40,IL-23p19 和IL-27p28 的mRNA表达,以及IL-12,IL-23 和IL-27蛋白的生成。其作用机制是PI-3 kinase和
ERK1/2信号通路诱导活化,抑制了调节IL-12家族蛋白表达的主要转录因子IRF-1和IRF-8。
TLRs也会和炎性体一起作用诱导IL-1和IL-8的分泌,这个过程主要包括两步:首先,TLR或NOD(nucleotide binding oligomerization
domain containing protein)信号通路诱导pro-IL-1和pro-IL-18转录。然后,通过炎性体的作用进行蛋白水解过程。第一步由TLR配
体或TNF-a诱导,第二步的信号由成孔毒素(pore-forming toxins),淀粉样肽(amyloid peptides),尿酸结晶(uric acid crystals)
和K+ ef�ux 发出。
DescriptionMM cat# Key Applications Species Reactivity Qty/Pk
抗体
05-591
05-1003
05-802R
06-74807-175
MAB3026
09-481
Akt/PKB Antibody, PH Domain, clone SKB1
phospho-Akt (Ser473) Antibody, clone 6F5
phospho-Akt (Thr308) Antibody, clone 50-1C-25, rabbit monoclonalJNK/SAPK1 Antibodyphospho-JNK (Thr183/Tyr185, Thr221/Tyr223) Antibody
NFκB Antibody, p65 subunit, active subunit, clone 12H11
PI3 Kinase Antibody, p110α
Flow Cytometry;Immunocytochemistry;Immunoprecipitation;Western BlottingELISA;Western Blotting;Flow Cytometry;Immunofluorescence;ImmunohistochemistryWestern Blotting;Immunofluorescence;Immunocytochemistry;ELISAImmunoprecipitation;Western BlottingWestern Blotting;Enzyme Assay;Immunoprecipitation
Electrophoretic Mobility Shift Assay;Flow Cytometry;Immunocytochemistry;Immunofluorescence;Immunohistochemistry;Immunohistochemistry (Paraffin);Western BlottingWestern Blotting;ELISA;Immunoprecipitation;Immunohistochemistry
Human;Mouse;Rat
Human;Mouse;Rat;Vertebrates
Human;Mouse;Rabbit;Bovine;Chicken
Human;Mouse;RatMouse;Human;Rat;Canine;Xenopus;Chicken;Bovine;HorseHuman;Mouse;Rat;Rabbit
Human;Rat
100 µg
100 µg
100 µL
200 µg200 µg
100 µg
100 µg
Nucleus
Bacterial Antigens
Growth Factors
Growth FactorReceptors
TNFα
Proteosome
TNFR1 IL-1R
IL-1
NF-κBNF-κBNF-κB
NF-κB
p50c-Rel
NF-κB
IκBs
HDAC3
p50
p50
p50Bcl3
p50
p105p105
ABIN-2Tpl2
LPS
TRADDTRAF2
p300
CBP
TRAF6
TRAF6
MALPPGN
MyD88TOLLIPMyD88
PI 3-K
PDK-1
ASK1
MKK4,7
JNKsAkt/PKB
IKK-γ
MEKKs
Tpl2
MEKK
ERK
PKR PKR
IRAKIRAK
IRAK
TAK1
IKK-α IKK-β
JNK PathwayERK
Signaling
Tpl2Degradation
Virus
IκB Degradation
Nuclear Import
SCF/β-TrCP
RelBp52NF-κB
IκBs
IκBs
p50c-Rel
NF-κB
IκBs p52p65
NF-κB
κ-BRas
IκBs p50p65
NF-κB
IκB
IκBs p52p65
NF-κB
PACT
Nuclear Export
AcetylationDeacetylation
Phosphorylation
Ubiquitin
Calbiochem® Inhibitors
51
DescriptionMM cat# Key Applications Species Reactivity Qty/Pk
抗体
05-53505-536
05-63107-1007
07-1008
06-1110MABC988
MABF349
MABF350
09-040
05-813
07-1483
05-53605-53506-1089ABF303
06-1115
06-87206-41305-1469
AB16528ABS1515
06-1340
ABS160
07-2192
07-1332
MABF84
06-1110
Anti-IKKβ Antibody, clone 10AG2Anti-IKKα Antibody, clone 14A231Anti-IKKγ AntibodyAnti-IKKα Antibody
Anti-IKKβ Antibody
Anti-TRAF6 AntibodyAnti-TRADD Antibody, clone 1G6.1 Anti-CD282 (TLR2) (human) Antibody, PE, clone TL2.1Anti-CD284 (TLR4) (human) Antibody, PE, clone HTA125Anti-c-Rel (NFκB) Antibody
Anti-HDAC3 Antibody, clone 3G6
Anti-IκBα Antibody
Anti-IKKα Antibody, clone 14A231Anti-IKKβ Antibody, clone 10AG2Anti-IL-12R-beta-1 AntibodyAnti-IL18R1 Antibody
Anti-Interleukin-18 (IL-18) AntibodyAnti-IRAK AntibodyAnti-NFκB p52 AntibodyAnti-NFĸB Antibody, p65, clone 1G10.2Anti-NIK Antibody, CTAnti-phospho NFkB p52, (Ser222) AntibodyAnti-phospho-IKK-epsilon (Ser172) AntibodyAnti-phospho-MKK4 Antibody (Ser257/Thr261)Anti-phospho-TBK1 (Ser172) AntibodyAnti-RelB (NFĸB family member) AntibodyAnti-TLR2 Antibody, clone 19B6.2
Anti-TRAF6 Antibody
Western BlottingWestern Blotting
Western BlottingWestern Blotting;Immunoprecipitation
Western Blotting
Western Blotting;ImmunohistochemistryWestern Blotting;Immunohistochemistry (Paraffin)Flow Cytometry
Flow Cytometry;Immunohistochemistry;ImmunofluorescenceELISA;Electrophoretic Mobility Shift Assay;Western BlottingImmunoprecipitation;Western Blotting;ImmunocytochemistryWestern Blotting;Immunohistochemistry (Paraffin);ELISA;ImmunoprecipitationWestern BlottingWestern BlottingWestern Blotting;ImmunohistochemistryWestern Blotting;Immunohistochemistry;ImmunocytochemistryWestern Blotting;Immunohistochemistry (Paraffin)Immunoprecipitation;Western BlottingImmunoprecipitation;Western BlottingWestern Blotting;Immunofluorescence;ImmunocytochemistryWestern BlottingWestern Blotting
Western Blotting
Western Blotting;Peptide Inhibition Assay
Western Blotting
Western Blotting;Immunocytochemistry;ImmunofluorescenceWestern Blotting;Immunohistochemistry (Paraffin);Flow CytometryWestern Blotting;Immunohistochemistry
Human;Rat;MouseHuman;Mouse;Monkey
Human;MouseHuman;Canine;Horse;Bovine;Rat;MouseHuman;Canine;Chicken;Bovine;Feline;MouseHuman;Horse;Rhesus MacaqueHuman;Rat
Human
Human
Human
Bovine;Human;Hamster;MouseHuman;Mouse;Rat
Human;Mouse;MonkeyHuman;Rat;MouseHumanHuman
Human;Chimpanzee;Rhesus Macaque;PrimateHuman;MouseHumanHuman;Mouse;Rat;Bovine
HumanHuman
Human;Mouse;Rat
Human;Mouse;Rat;Bovine;Xenopus;ZebrafishHuman;Mouse;Rat
Human
Human
Human;Horse;Rhesus Macaque
100 µg100 µg
100 µg150 µg
150 µg
100 µg100 µg
100 tests
100 tests
100 µg
200 µg
100 µL
100 µg100 µg100 µg100 µL
100 µg
200 µg100 µL200 µg
100 µg100 µL
100 µL
100 µL
100 µL
200 µL
200 µg
100 µg
试剂盒
IκB STAR ELISA Kit 17-485MILLIPLEX® MAP NF?B Magnetic Bead Kit 6-plex 48-630MAGMILLIPLEX® MAP Phospho NF-κB (Ser536) Magnetic Bead MAPmate™ 46-702MAGMILLIPLEX® MAP Total NF-κB Magnetic Bead MAPmate™ 46-701MAG
抑制剂(5Z)-7-Oxozeaenol, Curvularia sp. 499610BAY 11-7082 196870Histone Acetyltransferase Inhibitor II 382110IKK Inhibitor VII 401486Interleukin 1- Receptor-Associated-Kinase-1/4 Inhibitor 407601MEK Inhibitor II 444938NEMO-Binding Domain-Binding Peptide, Cell-Permeable 480025NF-κB SN50, Cell-Permeable Inhibitor Peptide 481480TIRAP Inhibitor Peptide, Cell-Permeable 613570Tpl2 Kinase Inhibitor 616373Trichostatin A, Streptomyces sp. 647925
52
Wnt SignalingWnt信号通路在进化中相当保守,参与细胞生命法则、发育、细胞增殖、细胞极化及迁移等过程。Wnt基因编码庞大的蛋白家
族,这些分泌性蛋白富含半胱氨酸,甚至在成体组织中对于发育和维持稳定也起着关键作用。如果这条信号通路中的关键蛋白
发生突变,导致信号异常活化,就可能诱导癌症和退行性疾病的发生。因此,对于这条信号通路的研究,需要一套小分子化合
物用于精确、完全的干扰调控,而InhibitorSelect™ Wnt信号通路抑制剂套装(含有15种小分子化合物)正是为应对这种实验要求
而设计的。
MM 匹配抗体名MM匹配抗体编号 Key Applications Species Reactivity Qty/Pk
抗体
MABD170MAB3786
05-1579
06-1050
MAB4350
ABE477MABS420
MABD122
MABE56907-1231
ABD13MABF108MABN709ABN40MABE69MAB3582
ABD71AB5687ABE1828ABE68
ABE73ABE7506-756ABE1351ABC217
07-1413
AB5702AB10050
06-1097MABC155MABD3507-1385MABD163
MABD102
MABS341AB5975AB5977
AB9220MABD24
AB5731MABC774
MAB5556
Anti-AFP Antibody, clone 6E6Anti-APC Antibody, CT, clone C-APC 28.9Anti-Axin-1 Antibody, clone A5
Anti-Axin-2 Antibody
Anti-BMP-7 Antibody, clone 2A10Anti-BRD2 AntibodyAnti-CACYBP, clone 7D11 AntibodyAnti-CD73 Antibody, clone AA60-E3-3
Anti-CK1, clone 3C10F7 AntibodyAnti-Notch1 Antibody, IntracellularAnti-Cripto-1 AntibodyAnti-CYLD Antibody, clone 16A1Anti-DKK1, clone 2H2 AntibodyAnti-DLK1 AntibodyAnti-Emerin Antibody, clone 7D9Anti-Fascin Antibody, clone 55K2
Anti-FOXC1 AntibodyAnti-FoxD3 AntibodyAnti-FOXK2 Antibody Anti-FoxP1 Antibody
Anti-FoxP2 AntibodyAnti-FOXP3 Antibody, clone 3G3Anti-Frizzled 5 AntibodyAnti-GATA-6 AntibodyAnti-GLI1 Antibody
Anti-GSK-3b Antibody, a.a. 335-349Anti-HES-1 AntibodyAnti-phospho-ITCH (pT222) AntibodyAnti-Jagged2 AntibodyAnti-KIF3A Antibody, clone 4F9.1 Anti-Klf4, clone 10E3.1 AntibodyAnti-Lin28 AntibodyAnti-LIN28 Antibody, clone 6D1F9Anti-Lin28 Antibody, clone PR3G10Anti-LRP6 Antibody, clone 1C10Anti-MAML1 AntibodyAnti-Musashi-1 Antibody
Anti-Nanog AntibodyAnti-NANOG Antibody, clone 7F7.1Anti-Nanog Antibody, NTAnti-phospho-NDRG1 (Ser330), clone EPR5594, Rabbit Monoclonal AntibodyAnti-Nicastrin Antibody
Western BlottingImmunofluorescence;Immunoprecipitation;Western Blotting;ImmunohistochemistryWestern Blotting;Immunoprecipitation;ImmunocytochemistryWestern Blotting;Immunohistochemistry (Paraffin)Western Blotting;Immunohistochemistry
Western Blotting;ImmunohistochemistryImmunohistochemistry
Immunocytochemistry;Flow Cytometry;Western Blotting;ImmunoprecipitationImmunohistochemistryELISA;Western Blotting;Immunohistochemistry (Paraffin)Western BlottingWestern BlottingWestern BlottingWestern Blotting;ImmunohistochemistryWestern Blotting;ImmunocytochemistryWestern Blotting;Immunocytochemistry;Immunohistochemistry (Paraffin)Western Blotting;ImmunocytochemistryWestern BlottingWestern Blotting;ImmunoprecipitationImmunocytochemistry;Western Blotting;ImmunohistochemistryWestern Blotting;ImmunohistochemistryWestern Blotting;ImmunocytochemistryWestern BlottingWestern Blotting;ImmunocytochemistryWestern Blotting;Immunohistochemistry (Paraffin)Western Blotting;Immunoprecipitation;Immunohistochemistry (Paraffin)Immunohistochemistry;Western BlottingDot Blot;Western Blotting
Immunohistochemistry (Paraffin);ELISAWestern Blotting;ImmunohistochemistryWestern Blotting;ImmunocytochemistryWestern Blotting;ImmunoprecipitationWestern Blotting;Immunofluorescence
Western Blotting;Immunocytochemistry
Western Blotting;ImmunoprecipitationWestern Blotting;ImmunohistochemistryImmunocytochemistry;Immunohistochemistry;Western BlottingFlow Cytometry;Western BlottingWestern Blotting;Immunocytochemistry
Western BlottingWestern Blotting;Immunohistochemistry
Immunoprecipitation;Western Blotting;Immunocytochemistry
HumanHuman;Mouse
Human;Mouse
Human
Human;Mouse
Human;RatHuman
Human
HumanHuman
HumanHumanHumanHuman;Mouse;RatHumanHuman;Mammals;Echinoderms;Gerbil;Sea UrchinHuman;MouseHuman;MouseHumanHuman;Mouse
Mouse;Rabbit;HumanHuman;Mouse;RatHuman;Mouse;RatHumanHuman;Rat
Rat;Human;Mouse;Bovine
Human;MouseHuman
HumanHuman;Mouse;RatHumanHuman;MouseHuman
Human
Human;Rat;MouseHuman;MouseHuman;Mouse;Rat
Human;MouseHuman
Human;Mouse;RatHuman
Human;Mouse;Rat
100 µL100 µg
100 µg
100 µg
100 µg
100 µg100 µL
100 µg
100 µg200 µL
100 µg100 µg100 µL100 µg100 µg100 µg
100 µg100 µg100 µL100 µL
100 µL100 µL200 µg100 µg100 µL
50 µg
100 µg100 µg
100 µg100 µg100 µg100 µL100 µL
100 µg
100 µg100 µg100 µg
100 µL100 µg
100 µg100 µL
100 µL
53
MM 匹配抗体名MM匹配抗体编号 Key Applications Species Reactivity Qty/Pk
抗体
ABN40MABE69MAB3582
ABD71AB5687ABE1828ABE68
ABE73ABE7506-756ABE1351ABC217
07-1413
AB5702AB10050
06-1097MABC155MABD3507-1385MABD163
MABD102
MABS341AB5975AB5977
AB9220MABD24
AB5731MABC774
MAB5556
Anti-DLK1 AntibodyAnti-Emerin Antibody, clone 7D9Anti-Fascin Antibody, clone 55K2
Anti-FOXC1 AntibodyAnti-FoxD3 AntibodyAnti-FOXK2 Antibody Anti-FoxP1 Antibody
Anti-FoxP2 AntibodyAnti-FOXP3 Antibody, clone 3G3Anti-Frizzled 5 AntibodyAnti-GATA-6 AntibodyAnti-GLI1 Antibody
Anti-GSK-3b Antibody, a.a. 335-349Anti-HES-1 AntibodyAnti-phospho-ITCH (pT222) AntibodyAnti-Jagged2 AntibodyAnti-KIF3A Antibody, clone 4F9.1 Anti-Klf4, clone 10E3.1 AntibodyAnti-Lin28 AntibodyAnti-LIN28 Antibody, clone 6D1F9Anti-Lin28 Antibody, clone PR3G10Anti-LRP6 Antibody, clone 1C10Anti-MAML1 AntibodyAnti-Musashi-1 Antibody
Anti-Nanog AntibodyAnti-NANOG Antibody, clone 7F7.1Anti-Nanog Antibody, NTAnti-phospho-NDRG1 (Ser330), clone EPR5594, Rabbit Monoclonal AntibodyAnti-Nicastrin Antibody
Western Blotting;ImmunohistochemistryWestern Blotting;ImmunocytochemistryWestern Blotting;Immunocytochemistry;Immunohistochemistry (Paraffin)Western Blotting;ImmunocytochemistryWestern BlottingWestern Blotting;ImmunoprecipitationImmunocytochemistry;Western Blotting;ImmunohistochemistryWestern Blotting;ImmunohistochemistryWestern Blotting;ImmunocytochemistryWestern BlottingWestern Blotting;ImmunocytochemistryWestern Blotting;Immunohistochemistry (Paraffin)Western Blotting;Immunoprecipitation;Immunohistochemistry (Paraffin)Immunohistochemistry;Western BlottingDot Blot;Western Blotting
Immunohistochemistry (Paraffin);ELISAWestern Blotting;ImmunohistochemistryWestern Blotting;ImmunocytochemistryWestern Blotting;ImmunoprecipitationWestern Blotting;Immunofluorescence
Western Blotting;Immunocytochemistry
Western Blotting;ImmunoprecipitationWestern Blotting;ImmunohistochemistryImmunocytochemistry;Immunohistochemistry;Western BlottingFlow Cytometry;Western BlottingWestern Blotting;Immunocytochemistry
Western BlottingWestern Blotting;Immunohistochemistry
Immunoprecipitation;Western Blotting;Immunocytochemistry
Human;Mouse;RatHumanHuman;Mammals;Echinoderms;Gerbil;Sea UrchinHuman;MouseHuman;MouseHumanHuman;Mouse
Mouse;Rabbit;HumanHuman;Mouse;RatHuman;Mouse;RatHumanHuman;Rat
Rat;Human;Mouse;Bovine
Human;MouseHuman
HumanHuman;Mouse;RatHumanHuman;MouseHuman
Human
Human;Rat;MouseHuman;MouseHuman;Mouse;Rat
Human;MouseHuman
Human;Mouse;RatHuman
Human;Mouse;Rat
100 µg100 µg100 µg
100 µg100 µg100 µL100 µL
100 µL100 µL200 µg100 µg100 µL
50 µg
100 µg100 µg
100 µg100 µg100 µg100 µL100 µL
100 µg
100 µg100 µg100 µg
100 µL100 µg
100 µg100 µL
100 µL
07-1232
07-1234
MABC594
MABN281
MABD76
MAB4305MAB4419
MAB4419A4
05-1573
ABN489
ABC478
Anti-Notch 1 Antibody, NT
Anti-Notch 2 Antibody, NT
Anti-NOTCH 3/N3ECD Antibody, clone 1E4Anti-Numb Antibody, clone 6G10.1Anti-Oct-4 Antibody, clone 9B7
Anti-Oct-4 Antibody, clone 9E3.2Anti-OCT-4 [POU5F1] Antibody, clone 7F9.2Anti-OCT-4 [POU5F1] Antibody, clone 7F9.2, Alexa Fluor® 488 conjugateAnti-Pax5 Antibody, clone 1H9
Anti-PEN2 Antibody
Anti-phospho-CYLD Antibody (Ser418)
ELISA; Western Blotting;Immunohistochemistry (Paraffin)ELISA;Western Blotting;Immunohistochemistry (Paraffin)Western Blotting;Immunocytochemistry;Electron MicroscopyWestern Blotting;Immunocytochemistry
Western Blotting;Immunohistochemistry;ImmunofluorescenceWestern Blotting;ELISAImmunocytochemistry;Flow Cytometry;Western BlottingImmunocytochemistry
Western Blotting;Immunohistochemistry;ImmunoprecipitationWestern Blotting;Immunohistochemistry;ImmunofluorescenceWestern Blotting
Human;Mouse
Human;Mouse
Human
Human
Mouse;Human
Human;Rat;MouseHuman;Mouse
Human;Mouse
Mouse;Human
Mouse;Rat
Human
200 µL
200 µL
100 µg
100 µg
100 µL
100 µg100 µg
100 µL
100 µg
100 µg
100 µL
54
MM匹配抗体名MM匹配抗体编号 Key Applications Species Reactivity Qty/Pk
抗体
InhibitorSelect™Wnt Signaling Pathway Inhibitor Panel,目录号681666组分 靶标 包装量 目录号
Casein KinaseⅠInhibitor,D4476Casein KinaseⅡInhibitorⅢ,TBCAβ-Catenin/Tcf Inhibitor,FH535GSK-3 Inhibitor IXPKGⅠInhibitor,Cell-PermeableH-89,DihydrochlorideJNK InhibitorⅡK-252a,Nocardiopsis sp.KN-93LY 294002(5Z)-7-Oxozeaenol,Curvularia sp.PP2RapamycinSB 202190UO126DMSO
Casein KinaseⅠCasein KinaseⅡβ-Catenin,Tcf GSK-3 PKGPKA,Rho KinaseJNK MultipleCaMKPI3-KinaseTAK 1Src FamilymTORp38MEK-
1 mg5 mg10 mg1 mg1 mg1 mg100 µg5 mg1 mg5 mg1 mg1 mg100 µg1 mg1 mg15 mL
218696218710219330361550370655371963420119420298422708440202499610529573553210559388662005KP31817
*以上组分可按目录号单独购买
抑制剂
07-393
ABS481
06-734
05-613
MAB2081
AB575709-706
MABD175
MABE167
AB5603
MAB4343
05-510
ABE598
ABE41405-511
ABD53
ABE596
MAB4381
MABN722
09-162MABD136
Anti-phospho-GSK3α (Ser21) AntibodyAnti-phospho Beta Catenin (Ser675) AntibodyAnti-β-Catenin Antibody
Anti-β-Catenin Antibody, clone 2H4A7Anti-β-Catenin Antibody, clone 5H10
Anti-Presenilin 1 AntibodyAnti-RING1 Antibody
Anti-SHH Antibody, clone 5H4
Anti-SNAI1 Antibody, clone 10H4.1Anti-Sox2 Antibody
Anti-SOX-2 Antibody
Anti-TCF-1 Antibody, clone 7H3
Anti-TCF12 Antibody
Anti-TCF-3 AntibodyAnti-TCF-4 Antibody, clone 6H5-3
Anti-ZEB1 Antibody
Anti-ZEB1 Antibody
Anti-TRA-1-81 Antibody, clone TRA-1-81Anti-WIF1 Antibody, clone 1G5
Anti-Wnt3A AntibodyAnti-Wnt-5a Antibody, clone 6F2
Western Blotting
Western Blotting;Peptide Inhibition Assay
Immunocytochemistry;Immunoprecipitation;Western BlottingImmunoprecipitation;WesternBlotting;ImmunohistochemistryImmunocytochemistry;Immunohistochemistry;Immunohistochemistry (Paraffin);Immunoprecipitation;Western BlottingELISA; Western BlottingWestern Blotting
Western Blotting;Flow Cytometry;ImmunohistochemistryWestern Blotting;Immunohistochemistry
Western Blotting;Immunocytochemistry;Immunofluorescence;ImmunohistochemistryImmunocytochemistry;Immunohistochemistry;Western BlottingImmunocytochemistry;Immunohistochemistry;Western BlottingWestern Blotting;Immunocytochemistry;ImmunohistochemistryWestern Blotting;ImmunohistochemistryElectrophoretic Mobility Shift Assay;Immunocytochemistry;Immunohistochemistry;Immunoprecipitation;Western BlottingWestern Blotting
Western Blotting;Immunocytochemistry;ImmunofluorescenceWestern Blotting;Flow Cytometry;Immunoprecipitation;Immunofluorescence;ImmunocytochemistryWestern Blotting;Immunohistochemistry;ImmunofluorescenceWestern Blotting;Immunohistochemistry (Paraffin)Western Blotting;Immunofluorescence;Immunohistochemistry
Human
Human
Human;Rat;Mouse;Rabbit;BovineHuman;Mouse;Rat
Human;Rat
Human;Mouse;RatHuman;Mouse;Horse;Bovine;Pig;CanineHuman;Mouse;Monkey
Rat;Mouse;Human
Human;Mouse
Human;Mouse
Human
Human
HumanHuman;Mouse
Mouse;Human;Bovine;Chicken;FrogHuman
Human
Human;Mouse
Human;Mouse;RatHuman;Rat
100 µL
200 µL
200 µg
200 µg
100 µg
100 µg100 µg
100 µL
100 µg
100 µg
100 µg
100 µg
100 µg
100 µg200 µg
100 µg
100 µg
100 µg
100 µL
100 µL100 µL
55
更多信号通路图及相关研究工具请咨询默克密理博公司或当地代理商
Autophage 信号通路Hedgehog 信号通路 p53 信号通路
IGF 信号通路 FGF 信号通路Wnt 信号通路
MAP 激酶信号通路 VEGF 信号通路NF-κB 信号通路
EGFR 信号通路 JAK/STAT 信号通路PI 3-K/Akt/mTOR 信号通路
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