Conflict of Interest Disclosure <Hidezo> <Mori>, <MD>

Has no real or apparent

conflicts of interest to report.

　 Visualization of Perforating Branches of Cerebral Arteries Toward Clinical Evaluation of Vascular Disease and Alzheimer’s-overlap

Syndrome

　 　 　 Hidezo Mori1, Naoto Fukuyama1, Yoshimori Ikeya1, Toshiharu Fujii1, Chiharu Tanaka1, Yuko Tsukamoto1, Shunya Takizawa1, Keiji Umetani 2.

1Departments of Physiology, Neurology and Cardiology, Tokai University School of Medicine, Ishehara, 2Division of Reserch and Utilization, JASRI, Sayo-cho, Japan

BackgroundVascular cognitive impairment (VCI) is characterized By sub-corticalwhite-matter lesion on MRI/CT, and often associated with Alzheimer Disease.

Purpose of Present Study

The present study examined whether

synchrotron radiation (SR) microangiography

can detect functional DM microangiopathy

and visualize directly the perforating

branches of cerebral arteries (PBCA) in rat

models.

An illustration of an experimental arrangementAn illustration of an experimental arrangement for synchrotron microangiography in ratsfor synchrotron microangiography in rats

Bending magnet

Synchrotron radiation

Single-clystal monochromator

Monochromatic X-Ray

object

X-Ray SaticonCamera

8GeV Electron Beam

Slitt

X-ray Shutter

線形加速器（０ ⇒１ ＧｅＶ）

Synchrotron （１ ⇒ ８ ＧｅＶ）

A

B

Langendorf

MI heart

Mass

　att

enuation　c

oeffi

cient　(c

m2/g

)

Photon　energy　(KeV)0　　　　　　　20　　　　　40　　　　　　60　　　　　80　　　　100　　　　120　　　　140

1000

100

10

1

0.1

bone

筋　肉

ヨード

K　 吸収端

M

ass

abso

rptio

n co

effic

ient

Photon　energy

K-edge

iodine

bone

muscle

(cm2/kg)

（ keV ）

Conventional white x-ray

Monochromatic x-ray

Mass Absorption Coefficient Plotted Mass Absorption Coefficient Plotted Against Photon Energy Against Photon Energy

　 Protocol-1 　 • In order to determine whether fingertip synchrotron

radiation (SR) microangiography has enough spatial resolution to quantitate arterioles (20-200 μm in diameter) , we measured diameter reduction as arteriolar branching in fingertip microvessels.

• Next, we compared the arteriolar diameter changes induced by Ach administration between control and DM rats.

b-2

a-1 a-2

100 μm

Microvessel of Fingertip

b-1

＊‡

#

‡ Daughter 1

＊ Mother

# Daughter 2

Baseline Ach

Lu

men

Dia

met

er o

f 1s

t D

aug

hte

r A

rter

y

Mother and 1st Daughter Artery

n = 38

Lu

men

Dia

met

er o

f 2n

d D

aug

hte

r A

rter

y

Mother and 2nd Daughter Artery

Baseline Ach Stress

Normal Group DM Group

n = 25 n = 37

Baseline Ach Stress

142.4 ± 61.9 190.9 ± 73.5 201.6 ± 83.0 160.4 ± 67.9

P < 0.016 P < 0.024

Protocol-2

• Whether SR microangiography can visualize perforating branches of cerebral arteries (PBCA) in rat models.

• By injecting contrast materials selectively into internal carotid artery SR microangiography was applied to visulaize cerebral microvessles in control and DM rats.

Perforating branches of cerebral arteries in normal ratPerforating branches of cerebral arteries in normal rat

Perforating branches of cerebral arteries in DM ratPerforating branches of cerebral arteries in DM rat

ConclusionConclusion

SR microangiography can detect functional

DM microangiopathy the both in fingertip arterioles

and perforating branches of cerebral arteries in

rats, and could be useful for clinical evaluation 　

of vascular disease and Alzheimer’s-overlap

syndrome.