水体中主要生物营养元素、水生生物的生态学关系及环境评价

2015

Coordinator: Liu Xiangjiang

主讲人：刘香江

实验性教学项目

An Ecological Survey of Nanhu南湖生态调查

Objectives To familiarize with the use of oceanographic

instruments To assess the water quality, distribution and

diversity of plankton and benthos To evaluate how gradient of organic

pollution affects the distribution and abundance of phytoplankton and zooplankton

Study sites – Nanhu

3 sites 6 stations on board

Station I

• Global positioning system (GPS) Exact coordinates of sampling station

(before and after sampling)

• Water depth (3 replicates) Use the echo sounder to measure water depth

• Water sampling Collect from: Surface (x1) Nutrients

Surface (x1),Middle (x1) and Bottom (x1, 1 m above bottom) Salinity, pH Jot down bottle number

Put bottle into the ice box

Station II

Zooplankton sampling- Haul the zooplankton net (125 μm mesh

size) from 2m above bottom to surface

- Rinse plankton net with filtered seawater

- Store and preserve sample with 35% formalin ( 5 drops) in white bottle

- Write down bottle number

Station III

Phytoplankton sampling

- Haul the phytoplankton net (80 μm mesh size) from 4 m deep to surface

- Rinse plankton net with filtered seawater

- Store and preserve sample with Lugol’s solution ( 5 drops) in dark bottle

-Write down bottle number

Station IV

Chlorophyll content

- Collect surface water with bucket- Filter 200 ml water- Add 5 drops of MgCO3 (shake before use)

- Store the filter paper in a labeled 15 ml centrifugal tube (label Group, Site & Filtered Volume)

- Wrap with aluminium foil icebox- Pass the filtered seawater to Station 2 & 3

Station V

Water clarity

- Measure water clarity with Secchi disk (do it 3 times)

Benthic organisms sampling- Use mud grab to collect the bottom sediment- Sieve to search for living organisms- Preserve benthos with ethanol in labeled sample bottles (Group & Site)

Station VI

Water temperature and Dissolved Oxygen (DO)

Use the multimeter to determine water temperature and DO concentration at

2 m interval when water depth > 10mor 1 m interval when water depth < 10m

Measure from 1 m above bottom to surface

Station flowRotation 1 Rotation 2

Gp1

Gp2

Gp3

Gp6

Gp5

Gp4

Gp6

Gp1

Gp2

Gp5

Gp4

Gp3

Site 1

Station flow

Important notes• Wear comfortable clothing, shoes (Don’t wear

sandals!)

• Bring• Hats and sun block• Sea sickness medication if necessary• Calculator• Camera • Umbrella