N

P

Tt

SSM

nM

ItohfvLtpbfptL8tflmp

h

P

Cw

E

Cmcsaataptdtewic2a

dctdwloo

h

P

St

RB

1

12

BtmciTdcttB

wdasosgibmrcp

Keywords: Bacillus thuringiensis subsp. israelensis; Bioinsecti-cide; Aedes aegypti; Cultural conditions; Deltaendotoxin

http://dx.doi.org/10.1016/j.nbt.2012.08.623

ew Biotechnology · Volume 29S · September 2012

oster 5.0.181

he influence of probiotic fermentation on Catharan-hus roseus functional properties

omayeh Moradi Moghadam, Anis shobirin Meor Hussin∗,huhaimi Mustafa, Seyed Hamed Mirhosseini, Mohd Yazid Abdulanap

Department of Food Technology, Faculty of Food Science and Tech-ology, University Putra Malaysia, 43400 UPM Serdang, Selangor,alaysia

n this study, investigation of the influence of probiotic fermenta-ion on the functional properties of the C. roseus extract was carriedut. Historically Catharanthus roseus also known as kemunting cinaad been used for various treatments. The use of probiotics in

ermented plants has become very popular in recent years for pro-iding health benefits in human beings. Lactobacillus acidophilusA5 and Lactobacillus casei-01 were used alternately to fermenthe C. roseus extract anaerobically at 37◦C for 9 days. Severalarameters such as pH, viable probiotic cell counts, changes in car-ohydrates and organic acids as well as antioxidant activity of theermented C. roseus extract was evaluated during the fermentationrocess. During the fermentation, Lactobacillus casei-01 producedhe lower pH and the viable counts of Lactobacillus acidophilusA5 and Lactobacillus casei-01 were 7.82 ± 0.015 log10 CFU/mL and.04 ± 0.040 log10 CFU/mL, respectively at the end of the fermen-ation. Both strains utilized glucose, fructose and sucrose duringermentation. The contents of organic compounds (citric, acetic,actic) were significantly (p < 0.05) affected by the types of the

icroorganisms. The study found that the fermentation had aositive effect on the antioxidant activity of the samples.

ttp://dx.doi.org/10.1016/j.nbt.2012.08.621

oster 5.0.182

ultivation of cyanobacteria Phormidium sp. in cassavaastewater

.C. Francisco∗, E. Jacob-Lopes, T.T. Franco

yanobacteria have been used for years in tertiary sewage treat-ent to eliminate pollutions compounds after organic matter by

onventional secondary treatment. The wastewater must receiveuitable treatment before being discharged into water bodiesnd several types of unit processes exist, but these are costlynd produce high sludge content. The main limitation in usinghese systems is related to the composition of the wastewaternd the possible presence of high concentrations of some com-ounds frequent in wastewaters from the agricultural industryhat can inhibit the growth. The objective of this study was toetermine the optimum concentration of wastewater of cassavao obtain biomass with cyanobacterium Phomidium sp. in het-rotrophic systems. Heterotrophic cultivation of cyanobacteriaas initially carried out in a 300 mL Erlenmeyer flask contain-

ng 150 mL medium under continuous shaking (200 rpm), initial

oncentration of inoculum of 100 mg/L, pH 7.6, temperature of6 ± 1◦C, absence of light and at dilutions of 5, 10, 15, 25, 50, 75nd 100% (v/v) of cassava wastewater using distilled water. The

ynamics of pH, cell concentration and consumption of organicarbon was evaluated every 24 hours during the growth phase ofhe microorganism. The results indicated that the dilution of 50%emonstrated the greatest potential in the biomass production,ith substrate yield coefficient of 0.69 mgbiomass/mgsubstrate in paral-

el to cell productivity of 8.4 mg/L h. These results show the abilityf cyanobacterium Phormidium sp. in convert significant amountsf organic compounds present in cassava wastewater.

ttp://dx.doi.org/10.1016/j.nbt.2012.08.622

oster 5.0.183

tudy of bioinsecticide production by Bacillushuringiensis israelensis

aida Zribi-Zghal1,∗, Marwa Kharrat1, Ahmed Rebaï2, Saoussenen khedr1, Slim Tounsi1

Biopesticides Team [L.P.A.P.], Centre of Biotechnology of Sfax, P.O. Box177, 3018 Sfax, TunisiaBioinformatics Unit, Centre of Biotechnology of Sfax, Sfax, Tunisia

. thuringiensis subsp. israelensis is the most effective microbial con-rol agent available to date against the larvae of dipterans such as

osquitoes and blackfly. This bioinsecticide is characterized by itsost-effectiveness, target specificity, ease of production, amenabil-ty to formulation, safety to non-target organisms and mammals.he major advantage of this bioinsecticides is that minor risk ofevelopment of resistance by mosquitoes, due to its multitoxinomplex. B. thuringiensis bioinsecticides are composed of a mix-ure of spores and crystals harvested from the culture media athe end of the fermentation. But the large scale production of thisacillus is expensive because of the high cost of the medium.

Medium optimization is required for each B. thuringiensis strainhich may have different response to nutritional and cultural con-itions. Therefore, an engineering approach is needed to ensuresuitable environment for growth and delta-endotoxin synthe-

is. In the present work, we attempted to compare the productionf bioinsecticides by too novel strains of B. thuringiensis israelen-is (BUPM98 and BLB461) highly active against mosquitoes. Ouroal is the development of a cost-effective medium, based onnexpensive, locally available raw materials for the production ofioinsecticide and the determination of the nutritional require-ent of the most potent strain against Aedes aegypti. The obtained

esults suggest that molasses and fish meal used as sources ofarbone and nitrogen, respectively, are more economical for theroduction of bioinsecticides by B. thuringiensis israelensis.

www.elsevier.com/locate/nbt S221