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Curriculum vitae

Jan Alexander Mennigen Assistant Professor in Comparative Physiology

University of Ottawa Department of Biology

Phone : (+001) 613-562-5800 x 6002 (office) (+001) 613-562-5800 x 6009 (lab)

Fax : (+001) 613-562-5486 Email: jan.mennigen@uottawa.ca Website : www.mennigen-lab.com

PERSONAL INFORMATION: Languages:

• English (fluent)

• French (fluent)

• German (fluent, mother tongue)

Citizenship:

• German Citizen

• Canadian Permanent Resident through the Opportunities Ontario Program for international PhD graduates from Ontario Universities (active since 03/05/2014)

EDUCATION:

• 2014-2016

University of Texas at Austin, United States Postdoctoral Fellow Project: Developmental and transgenrational effects of endocrine disrupting chemicals on the metabolic phenotype in the SD rat. Advisors: Dr. Andrea Gore and Dr. David Crews

• 2011-2013 Institut National de la Recherche Agronomique (INRA), France

Marie Curie Postdoctoral Fellow Project: Nutritional regulation and metabolic function of hepatic microRNAs in rainbow trout, Oncorhynchus mykiss. Advisor: Dr. Sandrine Skiba-Cassy

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• 2006-2011 University of Ottawa, Canada PhD in Biology PhD Thesis: Serotonin in the neuroendocrine brain of the goldfish, Carassius auratus: Pathways and potential disruption by SSRI pharmaceuticals.

Supervisors: Dr. Vance Trudeau and Dr. Tom Moon

• 2005-2006 Universität Stuttgart, Germany Diploma in Biology (German MSc equivalent) MSc Thesis: Fluoxetine disrupts the reproductive axis of female goldfish, Carassius auratus. Supervisor: Dr. Franziska Wollnik

• 2004-2005 Student exchange at the University of Ottawa, Canada Supervisor: Dr. Vance Trudeau

• 2000-2004 Universität Stuttgart, Germany Prediploma in Biology (German BSc equivalent) Honours thesis: Development and optimization of an ELISA-based detection assay for designer cytokines in mouse cancer models. Supervisor: Dr. Klaus Pfizenmaier

ADDITIONAL EMPLOYMENT/VOLUNTEERING/OUTREACH EXPERIENCE:

• Science Fair Judge for Oral and Poster presentations April 26th, Ashbury College, Ottawa Ontario, Canada (2017)

• Mentorship dinner participation and presentation and poster judge. 43rd Canadian Ecotoxicology Workshop. Sep 25th-28th, Shaw Conference Center, Edmonton, AB, Canada (2016)

• INRA Association pour le Développement des Activités Sociales (2011-2013)

• VP Communications, Biology Graduate Student Association, University of Ottawa (2007-2008).

• Internship Environment Canada (CWS) in Dr. Sean Kennedy’s lab, Ottawa, ON, Canada (2005). Project: Development of a real-time RT-PCR-based assay to determine herring gull age based on telomere length.

• Let’s Talk Science/Parlons Sciences (LTS) volunteer, Ottawa, ON (2004-2005).

• AGDW refugee teaching volunteer, Stuttgart, Germany (2003-2004).

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TEACHING EXPERIENCE: Classroom teaching experience: University of Ottawa, ON, Canada, Lecturer / Instructor (2016-2017)

• BIO 8361/BIOL 6304: Advanced Animal Physiology - Co-Instructor, 10 students. Advanced Animal Physiology is a seminar course that this year will focus on techniques used in comparative physiology. The goal is to introduce you to a variety of experimental techniques, focusing on the theory behind each technique, good practices using it, and the actual and potential applications of the technique in comparative physiology.

• BIO2110: Environmental Physiology (120 students). How representative individual healthy organisms respond to various natural or anthropogenic physical environmental parameters such as temperature, water, pH, electromagnetic radiation including UV, gases, pressure and heavy metals. Primary and secondary stress responses and homeostasis will be considered throughout. This course is intended primarily for students enrolled in the Environmental Science program.

• BIO4920B: 4th year seminar - Physiology Section, 30 students. Through lectures, student seminars, and/or group discussions, students learn how to critically evaluate the quality of the science in research publications.

• BIO8120: Directed Studies, Graduate Course, 1 student. Theoretical and practical approaches to study the involvement of microRNAs and DNA methylation in regulating gene expression in salmonid fish.

Université de Pau et des Pays de l’Adour Bayonne, AQ, France - Teaching Assistantship, lab demonstrator (2012)

• TP Biochimie L2 (Biochemistry, in French), 2nd year course (Dr. Salvado), 30 students. University of Ottawa, ON, Canada – Teaching Assistantship, lab demonstrator (2006-2010)

• BIO 1130 Introduction to Organismal Biology, 1st year course (Dr. Avaron), 40 students.

• BIO 1140 Introduction to Cell Biology, 1st year course (Dr. Avaron), 40 students.

• BIO 2127 Introduction to Plant Science Biodiversity to Biotechnology, 2nd year course (Dr. Heinermann), 40 students

• BIO 2133 Genetics lab, 2nd year course (Dr. Heinermann), 40 students

• BIO 3151 Molecular Biology, 3rd year course (Dr. Basso), 40 students

Woodroffe High School Ottawa, ON, Canada - Let’s Talk Science (2004/2005)

• Forensic Science/Molecular Biology module for grade 11 classes, 25 students Universität Stuttgart, BW, Germany – Teaching Assistantship, lab demonstrator (2003-2004)

• Einführung in biophysikalische Methoden der Forschung (Introduction to biophysical research methods), 2nd year course (Dr. Hülser), 8 students

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Supervision of graduate students: University of Ottawa – Supervision of graduate students (2017)

• Rubén Martínez López (co-hosted with Dr. Trudeau): Visiting student from Benjami Piña’s lab at the Spanish National Research Council Environmental Assessment and Water Research Centre in Barcelona, Spain.

• Dan Kostyniuk, MSc candidate: the nutritional regulation and metabolic function of hepatic microRNAs in rainbow trout, Oncorhynchus mykiss.

• Zeinab Altmieme, MSc candidate: The role of nonapeptides in reproductive behaviour in male cyprinid fish.

Supervision of undergraduate students: University of Ottawa – Supervision of Honour’s students (2016-2017)

• Adina Gotzmann: Nutritional and endocrine transcriptional regulation of key metabolic genes in liver and muscle of the North Pacific spiny dogfish, Squalus suckleyi.

• Rida Haider: The nonapeptide isotocin as mediator of reproductive endpoints in response to pheromone signalling in male goldfish, Carassius auratus.

• Mais Jubouri: Characterization of miRNA expression of candidate miRNAs in rainbow trout hepatocytes in response to glucose and insulin.

• Majd Haddad (co-supervised with Dr. Trudeau): miRNAs as mechansisms of paternal and maternal inheritance of stress response in zebrafish exposed to the pharmaceutical fluoxetine.

• Dan Kostyniuk (co-supervised with Dr. Gilmour): Effect of social status on lipid metabolism in rainbow trout, Oncorhynchus mykiss.

University of Ottawa – Supervision of Undergraduate Research Opportunity Program(UROP) students and volunteers (2016-2017)

• Manuela Fonseca (UROP 2017)

• Bihac Mazingh (Volunteer 2016)

• Vicotria Peters (Volunteer 2016) University of Texas at Austin – Supervision of volunteer students (2014-2016)

• Franz Puyol, Mercedes Munselle: Effects of a perinatal low-dose EDC mixture on the offspring’s metabolic phenotype in SD rats.

• Syed Zafar: Hypothalamic molecular mechanisms of the PCB mix A1221 in maternal-lineage dependent weight-gain in SD rats.

Institut National de la Recherche Agronomique – Co-Supervision of Baccalauréat professionnel students (2011-2013)

• Christopher Prochasson: Nutritional regulation of hepatic microRNAs in rainbow trout, Oncorhynchus mykiss.

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University of Ottawa – Co-Supervision of summer and Honours students (2006-2010)

• Ed Harris (Co-supervised with Dr. Moon). The effects of fluoxetine on food-intake in goldfish, Carassius auratus.

• Jake Zamora (Co-supervised with Dr. Trudeau). Effect of acute stimulation of the immune system on neuroendocrine control of reproduction in goldfish, Carassius auratus.

• Jamie Holden (Co-supervised with Dr. Moon). Effects of fluoxetine on the development of components of the stress axis in zebrafish, Danio Rerio.

• Agnes Crnic (Co-supervised with Dr. Trudeau). Modulation of the recently discovered reproductive peptides GnIH and kisspeptin by the serotonergic system in goldfish, Carassius auratus.

SCHOLARSHIPS AND AWARDS:

• John Armstrong award for best undergraduate thesis award the Department of Biology awarded to Dan Kostyniuk (co-supervised with Dr. Gilmour).

• Co-applicant on Dr. Harris’ NSERC RTI grant 2017: QTRAP facility for trace metabolite detection and identification (CAD 150,000 ).

• University of Ottawa grant 2017: Epigenetic determinants of metabolic phenotypes in teleost fish Epigenetic determinants of metabolic phenotypes in teleost fish (CAD 96,917)

• Ontario Ministry of Economic Development and Innovation grant 2017: Epigenetic determinants of metabolic phenotypes in teleost fish (CAD 219.576)

• Canada Foundation for Innovation Grant 2017: Epigenetic determinants of metabolic phenotypes in teleost fish (CAD 219.576)

• NSERC-Discovery grant (2017-2022): MicroRNAs as determinants of metabolic phenotypes in teleost fish (CAD 130,000)

• University of Ottawa, Faculty of Science Start-up grant (2017-2019) (CAD 200,000)

• Travel Award for Invited Speakers at the Presidential Symposium of the North American Society of Comparative Endocrinology 2015 (CAD 2,000)

• Winner of the Endocrine Society’s presidential poster Competition 2014 at the Endocrine Society’s annual meeting in San Diego

• European Union Marie Curie IEF Post-Doctoral fellowship for research at INRA St.Pée-sur-Nivelle, France, 2011-2013 (Salary and Research expenses, EUR 200,000)

• Travel grant by Health Canada for a workshop of the Pharmaceutical Advisory group, Niagara-on-the-Lake, Ontario, Canada, 2011 (CAD 200, declined)

• University of Ottawa Travel award for the NEED Symposium in Rouen, France, 2010 (CAD 650)

• International Neuroendocrine Federation Young Investigator Travel Award for the 7th International Conference of Neuroendocrinology in Rouen, France, 2010 (CAD 1000)

• University of Ottawa Travel award for Symposium on Pharmaceuticals in the Environment at the University of Guelph, Ontario, Canada, 2010 (CAD 200)

• University of Ottawa Graduate Student Association (GSAÉD) Travel Support award for 30th SETAC North America meeting in New Orleans, Lousiana, USA, 2010 (CAD 500)

• Intrinsik Award for best PhD student presentation, Laurentian SETAC AGM, Ottawa, Ontario, Canada, 2009 (CAD 200)

• Canadian Water Network Columbia River Field Workshop, Castlegar, British Columbia, 2009 (CAD 1,000)

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• EPCOR Travel Award/Hoar Award Finalist, Canadian Society of Zoology meeting in Toronto, Ontario, Canada, 2009 (CAD 500)

• University of Ottawa Travel Award for Canadian Society of Zoologists in Halifax, Nova Scotia, Canada, 2008 (CAD 500)

• PhD Comprehensive exam: Passed with distinction, University of Ottawa, Ontario, Canada, 2007

• PhD Admission scholarship, University of Ottawa, Ontario, Canada, 2006-2010 (tuition fees, valued at CAD 20,000)

• PhD International scholarship, University of Ottawa, Ontario, Canada, 2006-2010 (tuition fees valued at CAD 20,000)

• Ontario/Baden-Würtemberg Exchange scholarship OBW, Stuttgart, Germany, 2004-2005 (tuition fees valued CAD 10,000)

PEER-REVIEWED PUBLICATIONS (25):

(25) Best C, Best, Ikert H, Navarro-Martin L, Craig PM, Mennigen JA. Epigenetics in teleost fish: from molecular mechanisms to physiological phenotypes, submitted. (24) Mennigen JA, Thompson L, Bell M, Gore AC. Transgenerational effects of polychlorinated biphenyls: 1. Development and physiology across 3 generations of rats, submitted

(23) Mennigen JA, Volkoff H, Chang JP, Trudeau VL. The nonapeptide isotocin in goldfish: Evidence for serotonergic regulation and functional roles in the control of food intake and pituitary hormone release. General Comparative Endocrinology 10.1016/j.ygcen.2017.09.008.

(22) Mennigen JA, Zamora JM, Chang JP, Trudeau VL. Endocrine disrupting effects of waterborne fluoxetine exposure on the reproductive axis of female goldfish, Carassius auratus. Journal of Comparative Biochemistry and Physiology Part C Toxicology, Pharmacology and Endocrinology, 202:70-78. (21) Zhang D, Mennigen JA. 2015. Microtrout: A comprehensive, genome-wide miRNA target prediction framework for rainbow trout, Oncorhynchus mykiss. (20) Mennigen JA. 2015. Micromanaging metabolism: A role for microRNAs in energy metabolism in teleost fish. Invited review. Journal of Comparative Biochemistry and Physiology, Part A: Molecular and Integrative Physiology, S1096-4959: 160-8. (19) Geurden I, Mennigen JA, Plagnes-Juan E, Veron V, Cerezo T, Mazurais D, Zambonino-Infante J, Gatesoupe J, Skiba-Cassy S, Panserat S. 2014. High or low dietary carbohydrate:protein ratios during first-feeding affect glucose metabolism and intestinal microbiota in juvenile rainbow trout.Journal of Experimental Biology, 217: 3396-406. (18) Mennigen JA, Martyniuk CJ, Seiliez I, Panserat, S. Skiba-Cassy, S. Metabolic consequences of miRNA-122 inhibition in rainbow trout (Oncorhynchus mykiss). 2014. BMC Genomics, 15:70.

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(17) Mennigen JA, Martyniuk CJ, Seileiz I, Panserat S, Skiba-Cassy S. Metabolic effects of miRNA-122 inhibition in rainbow trout (Oncorhynchus mykiss) in vivo. 2013. Journal of Comparative Biochemistry and Physiology, Part B: Molecular and Integrative Physiology, 169:16-24. (16) Dai W, Panserat S, Mennigen JA, Terrier F, Dias K, Seiliez I, Skiba-Cassy S. Postprandial regulation of hepatic glucokinase and lipogenesis requires the activation of TORC1 signaling in rainbow trout (Oncorhynchus mykiss). 2013. Journal of Experimental Biology, 216-4483-92.

(15) Lado WE, Zhang D, Mennigen JA, Zamora JM, Popesku JT, Lewis JE, Trudeau VL. Rapid modulation of gene expression profiles in the forebrain of male goldfish following exposure to waterborne sex pheromones. 2013. General and Comparative Endocrinology, 192:204-13.

(14) Mennigen JA, Skiba S, Panserat S. Ontogenesis of expression of metabolic genes and microRNAs in of rainbow trout alevins during the transition from endogenous to exogenous feeding. 2012. Journal of Experimental Biology, 216:1597-1608. (13) Zhang D, Xi Y, Coccimiglio M, Mennigen JA, Jonz M, Ekker M, Trudeau VL. Morpholino knockdown of a novel short trans-membrane protein 1 (Stmap1) blocks mitochondrial respiratory activity in zebrafish. 2012. Physiological Genomics, 44:1133-1140. (12) Mennigen JA, Panserat S, Larquier M, Plagnes-Juan E, Medale F, Seiliez I Skiba-Cassy S. Postprandial regulation of hepatic microRNAs predicted to target the insulin pathway in rainbow trout. 2012. PLoS One, 7:e38604.

(11) Mennigen JA, Stroud P, Zamora JM, Moon TW and Trudeau VL. Pharmaceuticals as neuroendocrine disruptors. Lessons learned from fish on Prozac™. 2011. Journal of Toxicology and Environmental Health, Part B: Critical Reviews, 14:387-412.

(10) Popesku JT, Mennigen JA, Chang JP, Trudeau VL. Dopamine D1 receptor blockage potentiates AMPA-stimulated luteinising hormone release in the goldfish. 2011. Journal of Neuroendocrinology 23:302-309.

(9) Prindiville JS, Mennigen JA, Zamora JM, Moon TW, Weber JM. The fibrate drug gemfibrozil disrupts lipoprotein metabolism of rainbow trout. 2010. Journal of Toxicology and Applied Pharmacology, 251:201-208.

(8) Mennigen JA, Lado WE, Zamora JM, Chang JP, Moon T, Trudeau VL. Waterborne fluoxetine disrupts the reproductive axis in sexually mature male goldfish, Carassius auratus. 2010. Aquatic Toxicology, 100:354-364.

(7) Oakes KD. Coors A Escher B, Fenner K, Garric J, Gust M, Knacker T, Kuster A, Kussatz C, Metcalfe CD, Monteiro S, Moon T, Mennigen JA, Parrott J, Pery AR, Ramil M, Tarazona JV, Sanchez-Arguello, Ternes TA, Trudeau VL, Boucard T, Van der Kraak GJ, Servos M. 2010. An Environmental Risk Assessment for the serotonin Re-uptake inhibitor Fluoxetine – A case study utilizing the European Risk assessment framework. Integrated Environmental Assessment and Management, 6:524-539.

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(6) Zhao E, Grey C, Zhang D, Mennigen JA, Basak A, Chang JP, Trudeau VL. Secretoneurin (SN) is a paracrine factor from lactotrophs stimulating gonadotropin release in the goldfish pituitary. 2010. American Journal of Physiology - Regulatory, Integrative and Comparative Physiology, 299:1290-1297.

(5) Zhang D, Xiong H, Mennigen J, Popesku JT, Marlatt VL, Martyniuk CJ, Crump K, Cossins AW, Xia X, Trudeau VL. 2010. Defining global neuroendocrine gene expression patterns associated with reproductive seasonality in fish. PLoS One, 4:e5816.

(4) Mennigen JA, Sassine J, Trudeau VL, Moon TW. Effects of waterborne fluoxetine on food intake, weight and energy metabolism parameters in goldfish, Carassius auratus. 2010. Aquatic Toxicology, 100:128-137.

(3) Mennigen JA, Harris EA, Chang JP, Trudeau VL, Moon TW. 2009. The effect of fluoxetine on food intake and weight gain in female goldfish. Regulatory Peptides, 155:99-104.

(2) Mennigen JA, Martyniuk CJ, Crump K, Xiong H, Zhao E, Popesku JT, Anisman H, Cossins AR, Xia X, and Trudeau VL . 2008. The effect of fluoxetine on the reproductive axis of female goldfish, Carassius auratus. Physiological Genomics, 35:273-282.

(1) Popesku JT, Martyniuk CJ, Mennigen JA, Xiong H, Zhang D, Xia X, Cossins AR and Trudeau VL. 2008. The goldfish (Carassius auratus) as a model for neuroendocrine signalling. Journal of Molecular Endocrinology, 293:43-56.

CONFERENCE CONTRIBUTIONS / NON PEER -REVIEWED PUBLICATIONS (44):

(44) Mennigen JA*, Thompson L, Gore AC. PCB exposure on the reproductive axis in the rat. 2017. 44th Canadian Ecotoxicology Workshop 2017 Delta Hotel, Guelph, ON Canada. Oct. 1st-4th. (43) Mennigen JA, Panserat S, Marandel L. Hepatic miRNA profile in glucose-intolerant’ rainbow trout and predicted consequences for the integration of endocrine signals. 18th International Congress of Comparative Endocrinology (ICCE18). Lake Louise, AB, Canada Jun 4-9th. (42) Mennigen JA*, Panserat S, Marandel L. Diet‐induced hyperglycemia alters the hepatic microRNA profile in rainbow trout, O. mykiss. 2017. Canadian Society of Zoologists, Hochachka Satellite Symposium The University of Manitoba, Winnipeg, Manitoba May 13th-14th. (41) Kostyniuk D. Culbert B, Mennigen JA, Gilmour K. The effect of social hierarchies on lipid metabolism in rainbow trout. 2017. Undergraduate Honour’s Research project symposium, Ottawa, ON, Canada Apr. 29th. (40) Mennigen JA*. Novel molecular mechanisms underlying metabolic phenotypes in teleost fish. 2017. 3 Minute Thesis (Professors). Annual Ottawa-Carleton Institute of Biology symposium. Carleton University, Ottawa, ON, Canada. Apr. 27th-28th.

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(39) Kostyniuk D. Culbert B, Mennigen JA, Gilmour K. The effect of social hierarchies on lipid metabolism in rainbow trout. 2017. 14th Annual Ottawa-Carleton Institute of Biology symposium. Carleton University, Ottawa, ON, Canada. Apr. 27th-28th.

(38) Kostyniuk D. Culbert B, Mennigen JA, Gilmour K. The effect of social hierarchies on lipid metabolism in rainbow trout. 2017. 10th Annual Undergraduate Research Conference March Nipissing University, North Bay, ON, Canada. Mar. 24th-25th. (37) Kostyniuk D. Culbert B, Mennigen JA, Gilmour K. 2017. The effect of social hierarchies on lipid metabolism in rainbow trout. 26th Comparative Physiology and Biochemistry workshop, Keene, ON, Canada. Jan. 27th -29th. (36) Mennigen JA*. The pharmaceutical fluoxetine as endocrine disruptor in teleost fish. 2016. 43rd Canadian Ecotoxicology workshop. Shaw Conference Centre Edmonton, AB, Canada. Sep. 25th-28th. (35) Mennigen JA*. Endocrine regulation of microRNAs in teleost fish. 2015. Invited presidential symposium presentation at the presidential symposium at the 3rd Annual meeting of the North American Society for Comparative Endocrinology (NASCE) presidential symposium. Ottawa, ON, Canada, Jun. 21st-25th. (34) Thompson LM, Mennigen JA, Bell M, Tellez, M, Gore AC. Transgenerational effects of prenatal PCB exposure on the reproductive and metabolic phenotype in the SD rat.The 97th Annual Meeting of the Endocrine society. San Diego, CA, United States, Mar. 5th-8th. (33) Mennigen JA, Thompson LM, Gore AC. Transgenerational effects of prenatal PCB exposure on gene expression in the AVPV of rats. 2015. The 97th Annual Meeting of the Endocrine society. San Diego, CA, United States, Mar. 5th-8th. (32) Skiba S, Mennigen JA. Regulation and function of microRNA-122 in rainbow trout (Oncorhynchus mykiss). 2013. INRA Departmental meeting Physiologie animale et systèmes d’élevage (PHASE). Paris, France. Oct. 4th-5th.

(31) Trudeau VL, Lado WE, Zhang D, Mennigen JA, Zamora JM, Popesku JT, Lewis JE. Mapping neuronal pathways and gene expression networks underlying sex pheromone actions in male goldfish Mapping neuronal pathways and gene expression networks underlying sex pheromone actions in male goldfish. 2012. 7th International Symposium on Fish Endocrinology (ISFE), Buenos Aires, Argentina. Sept. 3rd-6th.

(30) Mennigen JA*, Panserat S, Larquier M, Plagnes-Juan E, Medale F, Seiliez I, Skiba-Cassy S. Postprandial regulation of hepatic microRNAs predicted to target the insulin pathway in rainbow trout. 2012. 15th International Symposium on Fish Nutrition and Feeding (IFNF), Molde, Norway. Jun. 4th-7th.

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(29) Lado WE, Mennigen JA, Zamora J, Popesku JP, Pranckeviciene E, Xia X, Lewis JE, Trudeau, VL. Gene expression profiles of male goldfish exposed to the priming sex pheromone 17-alpha,20-beta-dihydroxy-4-prgenen-3-one. 50th Annual meeting of the Canadian Society of Zoologists (CSZ), University of Ottawa, Ottawa, ON, Canada. May 16th-20th.

(28) Crcnic A, Lado WE, Mennigen JA, Lewis JE, Trudeau VL. Effects of serotonin on preoptic neurons implicated in the control of male goldfish reproduction. 2011. 50th Annual meeting of the Canadian Society of Zoologists (CSZ), University of Ottawa, Ottawa, ON, Canada. May 16th-20th.

(27) Crcnic A, Lado WE, Mennigen JA, Lewis JE, Trudeau VL. Effects of serotonin on preoptic neurons implicated in the control of male goldfish reproduction. 2011. Honour´s students´ poster presentation, University of Ottawa, Ottawa, ON, Canada. Mar. 25th.

(26) Zamora JM, Mennigen JA, Trudeau VL. Effect of acute stimulation of the immune system on neuroendocrine control of reproduction in goldfish, Carassius auratus. 2011. Honour´s students´ poster presentation, University of Ottawa, Ottawa, ON, Canada. Mar. 25th.

(25) Mennigen JA, Sassine J, Lado WE, Zamora JM, Moon TW and Trudeau VL. The effects of waterborne fluoxetine on the physiology of reproduction and feeding in goldfish: A mechanistic evaluation. 2010. 7th International Congress of Neuroendocrinology (ICN), Faculté de Droit, Université de Rouen, Rouen, France. Jul. 11th-15th.

(24) Trudeau VL, Mennigen JA, Wagh P, Popesku JT, Mimeault C, Lui G, Moon TW. Neuroendocrine disruption: More than fish hormones are upset. 2010. Environmental Science Research Initiative, Atrium New Science Complex, University of Guelph, ON, Canada. Feb. 27th.

(23) Mennigen JA, Sassine J, Lado WE, Zamora JM, Moon TW, Trudeau VL. The effects of waterborne fluoxetine on the physiology of reproduction and feeding in goldfish: A mechanistic evaluation. 2010. Pharmaceuticals in the Environment workshop. Environmental Science Research Initiative, Atrium New Science Complex, University of Guelph, ON, Canada. Feb. 27th.

(22) Mennigen JA*, Lado WE, Zamora JM, Chang JP, Trudeau VL. Sublethal effects of fluoxetine on the male reproductive axis in goldfish, Carassius auratus, 2009. 30th Annual meeting of the Society of Environmental Toxicology and Chemistry North America (SETAC), Hilton Riverside, New Orleans, LA, USA. Nov. 19th-23rd.

(21) Moon T, Prindiville J, Sassine J, Mennigen J, Weber J, Trudeau VL. Human pharmaceuticals disrupt feeding and fuel storage in fish. 2009. 36th Aquatic Toxicology Workshop. La Malbaie, Charlevoix, QC, Canada. Sept. 27th -30th.

(20) Zhao E, Basak A, Zhang D, Mennigen JA, Wong AOL, Trudeau VL. Secretoneurin is a paracrine factor stimulating gonadotropin release in goldfish pituitary under the control of gonadotropin releasing hormone (GnRH). 2009. 16th International Congress of Comparative Endocrinology (ICCE), Hong-Kong, China. Jun. 22th -26th.

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(19) Mennigen JA*, Moon TW, Trudeau VL. Sublethal effects of fluoxetine in reproduction and feeding in goldfish, Carassius auratus: Environmental implications. 2009. 14th Annual General Meeting and Conference of the Laurentian Society of Environmental Toxicology and Chemistry (SETAC), Ottawa, ON, Canada. Jun. 19th.

(18) Mennigen JA*, Zhao E, Zhang D, Moon TW, Trudeau VL. The effect of isotocin on the reproductive axis of the female goldfish, Carassius auratus. 2009. 48th Annual meeting of the Canadian Society of Zoology (CSZ), Scarborough, ON, Canada. May 15th.

(17) Mennigen JA, Trudeau VL. Physiological roles of isotocin in the reproductive phase of the female goldfish, Carassius auratus. 2008. 6th Annual symposium of the Ottawa-Carleton Institute of Biology (OCIB), Carleton University, Ottawa, ON, Canada. Apr. 30th-May 1st.

(16) Mennigen JA, Sassine J, Moon TW, Trudeau VL. The effects of waterborne fluoxetine on feeding and metabolic parameters in goldfish, Carassius auratus. 2008. 18th Annual conference on comparative physiology and biochemistry, Elmhirst’s Resort, Keene, ON, Canada. Feb. 6th-8th.

(15) Zhang D, Xiong H, Popesku J, Mennigen JA, Martyniuk C, Crump K, Xia X, Trudeau VL. Fishing for seasonal gene expression patterns in neuroendocrine brain from multiple microarray datasets. 2008. 16th Annual International Conference on Intelligent Systems for Molecular Biology. Metro Toronto Convention Centre, Toronto, ON, Canada. Jul. 19th-23rd.

(14) Moon TW, Mimeault C, Cameron C, Mennigen JA, Sassine J, Trudeau VL. Pharmaceuticals found in the aquatic environment affect steroidogenesis and feeding in fish. 2008. 6th International Symposium of Fish Endocrinology (ISFE), Calgary, AB, Canada. Jun. 22nd-27th.

(13) Trudeau VL, Popesku JT, Martyniuk CJ, Mennigen JA, Zhang D, Xiong H, Xia X, Cossins AR. Lipid- and cholesterol-lowering drugs and an anti-depressant modulate multiple pathways within steroidogenesis and reproduction in fish. Probing neuroendocrine signaling using transcriptomics. 2008. 47th Annual meeting of the Canadian Society of Zoologists (CSZ), Mount St. Vincent University, Halifax, NS, Canada. May 19th-23rd.

(12) Mennigen JA*, Harris E, Moon T and Trudeau VL. The effects of fluoxetine on food intake and growth in female goldfish, Carassius auratus. 2008. 47th Annual meeting of the Canadian Society of Zoologists (CSZ), Mount St. Vincent University, Halifax, NS, Canada. May 19th-23rd.

(11) Harris E, Mennigen JA, Moon T and Trudeau VL. The effects of fluoxetine on food intake and growth in female goldfish, Carassius auratus. 2008. Honour´s student poster presentation, University of Ottawa, Ottawa, ON, Canada. Mar. 28th.

(10) Mennigen JA, Popesku JT, Martinyuk C, Xiong H, Zhang D, Xia X, Cossins A, Moon T, Trudeau VL. Neurotransmitter regulation of gene expression in the neuroendocrine brain of goldfish (Carassius auratus): Emerging role for isotocin in reproduction. 2008. 26th Canadian Workshop on Human Reproduction and Reproductive Biology. Ottawa, ON, Canada. May 14th.

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(9) Mennigen JA, Popesku JT, Martinyuk C, Xiong H, Zhang D, Xia X, Cossins A, Moon T, Trudeau VL. Neurotransmitter regulation of gene expression in the neuroendocrine brain of goldfish (Carassius auratus): Emerging role for isotocin in reproduction. 2008. Ottawa-Carleton Institute of Biology Symposium, University of Ottawa, Ottawa, ON, Canada. Apr. 28th - 29th.

(8) Mennigen JA*, Harris E, Moon T and Trudeau VL. The effects of fluoxetine on food intake and growth in female goldfish, Carassius auratus. 2008. 17th Annual conference on Comparative Physiology and Biochemistry, Elmhirst’s Resort, Keene, ON, Canada. Feb 8th-10th.

(7) Trudeau V, Popesku J, Mennigen JA, Basu N, Martel P, Kovacs T, Hewitt M, Milestone C, Sherry J, Parrott J, Arnason J. 2007. Endocrine Disruption: More than just hormones. 7th Annual Congress of the Society of Environmental Toxicology and Chemistry Latin America (SETAC), Montevideo, Uruguay. Oct. 8th-11th.

(6) Moon T, Mimeault C, Woodhouse A, Cameron C, Estey C, Bullock L, Mennigen JA, Trudeau VL. Lipid- and cholesterol-lowering drugs and an anti-depressant modulate multiple pathways within steroidogenesis and reproduction in fish. 2007. 34th Annual Aquatic Toxicity Workshop, Halifax, NS, Canada. Sep. 30th-Oct. 3rd.

(5) Khalouei S, Yao X, Mennigen JA, Carullo M, Ma P, Song Z, Xiong H, Xia X. Bioinformatic Approach to Identify Penultimate Amino Acids Efficient for N-Terminal Methionine Excision. 2007. 1st International Conference on Bioinformatics and Biomedical Engineering. Wuhan University, Wuhan, China. Jul. 6th-8th.

(4) Mennigen JA, Martyniuk C, Crump K, Marlatt V, Xiong H, Woodhouse A, Nadler A, Anisman H, Xia X, Moon T, Trudeau VL. The effect of fluoxetine on the reproductive axis of female goldfish, Carassius auratus. 2007. Ottawa-Carleton Institute of Biology (OCIB) Symposium, Carleton University, Ottawa, ON, Canada. May 1st -2nd.

(3) Mennigen JA, Martyniuk C, Crump K, Marlatt V, Xiong H, Woodhouse A, Nadler A, Anisman H, Xia X, Moon T, Trudeau VL. The effect of fluoxetine on the reproductive axis of female goldfish, Carassius auratus. 2007. Pharmaceutical and Personal Care Products in the Canadian Environment: Research and Policy Directions, Queen’s landing Inn, Niagra-on-the-Lake, ON, Canada. Mar. 5th-7th.

(2) Mennigen JA*, Martyniuk C, Crump K, Marlatt V, Xiong H, Woodhouse A, Nadler A, Anisman H, Xia X, Moon T, Trudeau VL. The effect of fluoxetine on the reproductive axis in female goldfish, Carassius auratus, 2007. 16th Annual conference on comparative physiology and biochemistry. Elmhirst’s Resort, Keene, ON, Canada. Feb. 2nd-4th.

(1) Trudeau VL, Cameron C, Mennigen JA, Martyniuk C, Marlatt V, Moon T. Pharmaceuticals In The Aquatic Environment: Drugged Fish? 2006. 23rd Conference of European Comparative Endocrinologists, Manchester, UK. Aug. 29th-Sep. 1st.

* Represents personal oral presentations

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INVITED SEMINARS (7):

(7) Mennigen JA. MicroRNAs: Small molecules with large potential in teleost aquaculture. 2017Milieux et Resources Aquatiques Semianr Series University of Pau et Pay d’Adour. Bayonne, AQ, France. Sep. 28th. (6) Mennigen JA. The regulation of teleost energy metabolism: Novel molecular mechanisms and disruption by aquatic contaminants. Workshop on Fish Nutrition and Endocrinology. I Institute of Hydrobiology (IHB) of Chinese Academy of Sciences, Wuhan, HB, China. Aug 25th (5) Mennigen JA. Developmental and trans-generational effects of endocrine disrupting chemicals.2015. Baylor University, Waco,TX, United States. Feb 25th. (4) Mennigen JA. The endocrine regulation of metabolism and its disruption by EDCs in vertebrates. 2015. University of Ottawa, ON, Canada, Jan 21st. (3) Mennigen JA. The SSRI fluoxetine, a (neuro)endocrine disrupting chemical? 2009. Physiology Seminar Series, University of Ottawa, Ottawa, ON, Canada. Feb. 11th.

(2) Mennigen JA. Disruption of the serotonergic regulation of the endocrine brain of female goldfish through the SSRI pharmaceutical fluoxetine. 2008. Canadian Wildlife Service Seminar Series National Wildlife Research Centre (NWRC), National Wildlife Research Centre, Raven Road, Carleton University, Ottawa, Ontario, Canada. Dec. 10th. (1) Mennigen JA. Prozac in the environment: Update from Canada. 2006. ERAPharm Workshop, Viamede Resort, Peterborough, ON, Canada. Oct. 3rd.

PROFESSIONAL AND PUBLIC SERVICE:

PhD Comprehensive exam committee member:

• Keegan Lutek, PhD candidat University of Ottawa (2017)

• Carol Best, PhD candidate University of Ottawa (2016)

• Myy Mikwar, Phd Candidate Health Canada, Carleton University (2016)

Evaluation of graduate student theses and thesis defence committee member:

• Eric Turenne, MScUniversity of Ottawa (2017)

• Maria Vu, MSc University of Ottawa (2017)

• Rami Yassine, MSc University of Ottawa (2017)

• Marissa Northorp, MSc University of Ottawa (2017)

• Megan Jung, MSc University of Ottawa (2017)

• Dillon DaFonte, MSc University of Ottawa (2016)

• Marie-Ève Bélair-Bambrick, MSc University of Ottawa (2016)

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Evaluation of Undergraduate student theses:

• Prinica Anney, Honour’s thesis University of Ottawa (2017)

• Dan Kostyniuk, Honour’s thesis University of Ottawa (2017)

• Christopher Prochasson, Rapport de stage, Baccalauréat professionnel, INRA (2013)

• Jake Zamora, Honour´s thesis University of Ottawa (2011)

Graduate student committee member:

• Jonathon Forbes, MSc Universiry of Ottawa (2017)

• Delaney Large, CWS, Environment and Climate Change (2017)

• Ibragim El-Sakli, MSc candidate University of Ottawa (2017) Ad-hoc reviewer of articles for scientific journals:

• Archives of Womens’ Mental Health (1)

• Aquatic Toxicology (2)

• British Journal of Nutrition (1)

• BMC Genomics (2) • Developmental and Comparative Immunology (2) • Ecotoxicology (1)

• Endocrinology (1)

• Endocrine Reviews (1)

• Environmental Pollution (2)

• Fish and Shellfish Immunology (2)

• Fishes (1)

• Gene (1)

• General and Comparative Endocrinology (8)

• International Journal of Biological Science (1)

• Molecular and Cellular Endocrinology (1)

• PeerJ (1)

• The British Journal of Nutrition (1)

• The Journal of Aquatic Toxicology (2)

• The Journal of Comparative Physiology and Biochemistry, Part B: Biochemistry and Molecular Biology (6)

• The Journal of Comparative Physiology and Biochemistry, Part C: Pharmacology, Toxicology and Endocrinology (1)

• The Journal of Comparative Physiology and Biochemistry, Part D: Genomics and Proteomics Biology (1)

• The Journal of Clinical Endocrinology and Metabolism (1)

• The Journal of Experimental Biology (4)

• Toxicological and Environmental Chemistry (1)

• The Journal of Fish Biology (1)

• The International Journal of Fisheries and Aquaculture (1)

• The Journal of Toxicology and Environmental Health, Part B: Critical reviews (1)

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Conference organization and session chairing:

• Local organizing committee (Sponsorship) 10th International Congress of Comparative Physiology and Biochemistry ICCPB2019

• Co-organizer of the 27th Comparative Physiology and Biochemistry workshop, 2018 in Keene, ON (with Dr. Standen, University of Ottawa)

• Organizing and co-chairing a session on Endocrine disrupting chemicals at the 44th Canadian Ecotoxicology workshop in Guelph (with Dr. Craig, University of Waterloo)

• Chairing a student session of the 19th Annual Conference on Comparative Physiology and Biochemistry, 2010. Elmhirst’s Resort, Keene, ON, Canada. Feb. 5th-7th.

Professional Memberships:

• Society of Environmental Toxicology and Chemistry (SETAC)

• Canadian Society of Zoologists (CSZ)

• Canadian Water Network (CWN)

• North American Society for Comparative Endocrinology (NASCE)

• The Endocrine Society (ENDO)

IMPACT:

Citation indices:

Citations: 829 h-index: 15 i10-index: 17

(Google scholar, Sep. 2017)

Additional impact:

• Mennigen JA. The article ‘Micromanaging metabolism-a role for miRNAs in teleost energy metabolism’ published in The Journal of Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology was featured in the journal’s 25 most dowlnloaded article list between October 2015 and December 2015.

• Mennigen JA. The article ‘Waterborne fluoxetine disrupts the reproductive axis in sexually mature male goldfish, Carassius auratus’ published in Aquatic Toxicology was featured in the journal’s TOP 25 Hottest Articles list between October 2010 and March 2011.

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• Mennigen JA. The article ‘Waterborne fluoxetine disrupts the reproductive axis in sexually mature male goldfish, Carassius auratus’ published in Aquatic toxicology featured in regional and international radio, print media, and the Internet. Selected examples include: The Ottawa Citizen, CBC News Radio (Canada), La Presse Montreal (Canada), Agence France Presse (France), The Independent (U.K.), India Times (India), World News SBS Australia (Australia).

• Mennigen JA and Trudeau VL featured on a televised CTV special on pollutants in the environment, 2008. 6 PM news Ottawa, Feb. 19th.

• Mennigen JA. The article ‘The effect of fluoxetine on the reproductive axis of female goldfish, Carassius auratus’, published in Physiological Genomics was cited in the ‘Petition for water quality criteria for endocrine disrupting chemicals under section 304 of the clean water act, 33 U.S.C. 1314 presented to the U.S. Environmental Protection Agency (EPA) by the Center for Biological Diversity.

• Mennigen JA. The abstract ‘The effect of fluoxetine on the reproductive axis of female goldfish, Carassius auratus’ from the Pharmaceuticals and Personal Care Products (PPCPs) in the Environment workshop, 2007 cited in the Chartered Institute of Environmental Health report on challenges and opportunities in studying ecologic and population health impacts of endocrine toxicants (2008).

• Mennigen JA. The abstract ‘The effect of fluoxetine on the reproductive axis of female goldfish, Carassius auratus’ from the PPCPs in the Environment workshop cited in the National Water Research Institute (NWRI) Assessment report Series No. 8, Environment Canada.