Đề cương nghiên cứu khoa học ( nhóm 2)

8/3/2019 cng nghin cu khoa hc ( nhm 2)

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B CNG THNGTRNG H CNG NGHIP THC PHM TP. HCM

Nhm 6:1. L Minh Chc2.Nguyn Th Ho

3.NguynQuc Hiu4. Trn Th nh Ly5. Thiu Th Bch Mai6. L Th Ngc M

7.Nguyn ThKiu Th

Ti: CNG NGHIN CUKHOA HC

GV: nh Nguyn Trng Ngha

1

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NI DUNG BO CO

I. CNG NGHIN CU KHOA HC

1.1. nhngha

1.2. Phn loi

1.3. Phng php xy dng

1.4. cngtng qut

1.5. cng chi tit

II. CNG CHI TT C TH

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L bn m t thitk, khoch v kinh phcamtchnghin cu

HayL bn m t chi titcamtchnghin cunhmnh

gi mtvnno .


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C 2 loicng NCKH

- cng tng qut (project concept hay conceptnotes): l cngthhin tng nghin cu.

- cng chi tit (research project proposal): l cngthchin cng vic.

1.2.Phn loi


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L cu hi m chng ta c thtrli thng qua thu nhp vphn tch sliu.

Quan stCu hi

GithuytDonktqu

t cuhi: L

nghthuthnh thnh

cu trc

githuytv d

on ktqu

I. CNG NGHIN CU KHOA HC1.3.Phng php xy dng

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*Githuyt l mtsgii thchphngonsbvmt quan st, mthintng hay mtvn khoa hc

m c th nghin cubngnhng quan st su, nhngiu tra, hay php thnghim.

*Hnh thnh mtgithuyttts:

-Tp trung c suy ngh v nh hnh nghin cung

-Trnh mtthi gian nghin cu/ quan st

-Gim chi ph khi nghin cu

-Gimmuvt khi nghin cu


tgithuyt

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-S t m ham hiubit, s mong mun tmhiu (vd: ti sao v nhth no m mtngvt

hay thcvthotngc?)-Quan st v tnh hay ngu nhin (vd: quan sttp tnh camtngvt bn ngoi t nhin haynui nht)

-Quan st vimcch tm hiu-Cc nghin cutrc: quan st c c l xut

pht t cc nghin cutrc, kho st trc thng

qua vic tm hiumtvn khc m c trongti liu tham kho.


Ngungcca cu hi nghin cu

1.3.Phng php xy dng

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Cu trc bao gm:

- Tn ti

- Ngich tr v ngiphihp(cng s)

- Giithiu

- Mc tiu

- Ni dung nghin cu

- Ktqu mong i- Khochthchin


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1.4.1.Tn ti-Ngngn, thhincmc tiu, ni dung v ktquk

vng-T quan trngttrc, trnh ttha

-Da vo quan st v githuytt tn ti1.4.2.Ngich tr/phihp-Tn ngich tr (chnhim ti)-Tn ngiphihpthchin ti

1.4.3. Giithiu/tvn-Nu nhngvn chnh lin quan n ch qua lckho ti liu c lin quan

-Nu ln c nhu cu cn thit ca ti t gipngichiucti saophi nghin ti ny

1.4 cngtng qut


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1.4.4.Mc tiu-Mc tiu tng qut (mc tiu lu di): nhngiu m ti

sau khi thchinsng gp cho mtmc tiu ln.-Mc tiu cth(mc tiu trcmt, mc tiu ngnhn): l

iu m ti stc sau khi trin khai nghin cu-Khi vitmc tiu trnh dng nhng tch hnh ngu

cu (vd:iu tra, kho st, nh gi,)1.4.5.Nidung

-Gip ngic hnh dung khilng cng vicca tiv xem xt c ph hpvimc tiu v tn ti hay khng.-Lit k cc ni dung m ti dkins tin hnh, lu

ch nu nhngni dung chnh m chacn nu c th tng thnghimca nghin cu

1.4 cngtng qut


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6.Ktqu mong i

-Lit k nhngktquc th m ti mong istc (so vi mc tiu th kt qu mong i c tnh c thhn)7.Ktquthchin

-Nu tng qut khochthi gian ca ti (thytin v tnh kh thi). C th dng dngs trnh by, nnnu khoch theo cc ni dung nghin cuca ti8.Dton kinh ph

-Nu cc mc kinh ph chnh (chacn tnh ton chi tit)

c th c muhngdn km theo9.Ti liu tham kho

-Lit k tt c cc ti liu tham kho dng vit cng


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Cu trc cnggm

1.5.1. Tn ti1.5.2. Ngi ch trphihp

1.5.3. Gii thiu

+Giithiu chung

+Mc tiu v githuyt+Ni dung nghin cu

+Ktqu mong i

1.5.4. Phng php nghin cu1.5.5. Khochthchin

1.5.6. D ton kinh ph

1.5.7. Ti liu tham kho

1.5 cng chi titI. CNG NGHIN CU KHOA HC

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1.5.1.Tn ti (gingnh cch t tn ti NCTQ)

1.5.2.Ngich tr/phihp-Tn ngich tr (chnhim ti)

-Tn ngiphihpthchin ti

1.5.3.Giithiutvn-Giithiu chung

+Giithiungngn cc vn lin quan ti ti

+Ni c tnh cpthitti sao chn ti ny

-Mc tiu ca ti:+Mc tiu tng qut v mc tiu cth(nhng ti nh cth khng cn nu mc tiu tng qut). VD: nhmbithayhiucsnhhngcavicsdngha chtnsc

khe c tra nui trng ao

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1.5.3.Giithiutvn

-Gi thuyt: gi thuyt l iu m ti t ra thc hin

nghin cunhm chng minh iu nu ra l ng hay sai chp

nhn hay bacbgithuytt ra

VD: Cho c nhiu ln trong nms khng nhhngn

chtlngc btsinh ra?

-Ni dung nghin cu: litk cc ni dung chnh m ti s

thchinchchacn chi tit ha ni dung s nghin cu.

VD: phn tch sbinng li sutca ngn hng trong nm

2012 (Khng cn nu r l cch thu thpsliu nhthno)

1.5 cng chi tit


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1.5.3.Giithiutvn

-Ktqu mong i: Lit k cc ktqucth m tikvngstc sau khi thchin nghin cu

VD: Xc nh c cc nguyn nhn gy ra s binng v gi mua c tra v tm s BSCL trong nm2012

Hay

Xc nhc nguyn nhn gy ra bnh trnguitm cng xanh sau khi ti ktthc

1.5 cng chi tit


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1.5.4. Phng php nghin cu (PPNC)

-L phn quan trngnhtcacng, PPNC ngs toschnh xc v lng tin cangicviktqu nghin cu.

-PPNC phicvit chi tit, lm sao c thchiu v trinkhai c cng vic.

VD+Thnghim: m ttng thnghimnhsnghimthc, sln

lpli, muvt, vtt ha cht, iukin thnghim, chm sc vqun l, cch lymu( slng, sln,mc chnh xc cadngclymu,), chs cng thc tnh ton,

+iu tra phng vn: S mu iu tra, cch chn mu, aimchniu tra, chunb v thbiumu, tphunvbiumu,

phng php x l sliu(chs v cc cng thc tnh,phnmmsdng,)

I. CNG NGHIN CU KHOA HC1.5 cng chi tit

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1.5.5. Khochthchin

-Nu r thi gian cho tng cng viccth (kcx lsliu v vit bo co,phi nu r v chi tithnivicngtng qut)

-Nn dng sbiuhin1.5.6.Kinh ph

-Tnh ton chi tit cho tngmc chi nh cng lao ng(lng), vt t ha cht, my mc thitb, thu mn

chuyn mn, hcbng, hc ph,(c hng dn theongi ti trkinh ph)

1.5.7.Ti liu tham kho

-Lit k tt c ti liu tham kho (TLTK) dng vit

cng

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II. CNG CHI TT C TH

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2.1. Tn ti

NghipvBiuhin gen Cry1Ac khng su thucbcnh vy (lepidoptera) trn cy c chua (Licopersicon

esculentum Mill.)

2.2. Ngich tr phihp1. TS. Dng Hoa XTT CNSH TP. HCM2. TS.NguynQuc BnhTT CNSH TP. HCM

3. TS. VThoTT CNSH TP. HCM4. ThS. H Th LoanTT CNSH TP. HCM5. CN.NguynThNgcThoTT CNSH TP. HCM

II. CNG CHI TT C TH

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Giithiu chung

Vicsdng cc lai thuc tr su ny aphn c ngungcha hc v chng gy ra nhiu tc ng tiu ccn mi trng, sckhe con ngi v vt nui. Mt trong nhngphng n thay thcho vicsdngthuctr su ha hc l sdngthuctr su cngungc vi sinh vt v thcvt.

V vymt cch tipcn khc trong vichnch subnh trncy c chua l to cy chuyn gen mang vbiuhin gen ctB.thuringiensisnh mbov cy khis ph hoi trong sut qutrnh sinh trng. Do chng ti tin hnh nghin cu ti:Biuhin gen Cry1Ac khng su thucb cnh vy (lepidoptera)trn cy c chua (Licopersicon esculentum Mill)

II. CNG CHI TT C TH

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Mc tiu tng qut:

Nghin cutomtsging c chua (Licopersicon esculentumMill) mang gen Cry1Ac c khnng khng su thucb cnhvy (lepidoptera)

Mc tiu cth:Mc tiu nm 2011

- V mu ht mt s ging c chua lm ngun vt liuchuyn gen.

- Gy nhim vi khunAgrobacterium tumefaciens c chaplasmid mang gen Cry1Ac, gen bar v gen gus vo ccbphn

khc nhau trn cy c chua trong iukin in vitro.Mc tiu nm 2012Sng lc v ti sinh cc dng c chua chuyn gen thnh

cng.nh gi khnng khng su thucb cnh vy lepidoptera

ca cy c chua chuyn gen thu nhnc

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Ni dung nghin cu

Tng quan v cy c chua

Su, bnh hi trn cy c chuaTnh hnh sn xut c chua trn th giiTnh hnh nghin cu chn to ging c chua Nghin cu chuyn genBttrn cy c chuaGii thiu c t tr su ca vi khunBacillus ThuringinesisGiithiu vi khunBacillus thuringiensisPhn loi protein CryCchhotngcactGiithiu vi khunAgrobacterium

2.3. Giithiu

II. CNG CHI TT C TH

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Ktqu mong i

Cnc trn cc ni dung thchinca ti, chng tidkinktqunh sau:

Thu nhn c ngun vt liu chuyn gen khng suthucb cnh vy (lepidoptera)bngkthut in vitro.Hon thin quy trnh chuyn gen cy c chua khng su

bng vi khunAgrobacterium tumeficient.Tm c mi trng nui cy cy c chua chuyn gentiu.Thu c cc dng c chua chuyn gen c kh nng

khng su thucb cnh vy (lepidoptera)

II. CNG CHI TT C TH

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2.4 Phng php nghin cu

II. CNG CHI TT C TH

Tongunvtliuchuyn gen.Gy nhim vi khunAgrobacterium tumefaciens c cha

plasmid mang gen Cry1Ac, gen bar v gen gus vo ccbphn khc nhau trn cy c chua trong iukin in vitro.

Sng lc v ti sinh cy c chua chuyn gen thnhcng.

nh gi kh nng khng su thuc b cnh vy(lepidoptera) ca cy c chua chuyn gen.

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2.5. Khochthchin

TTCc ni dung, cng vic thc hin chyu

Thi gian

(Bt u-Kt thc)

1 Tongunvtliuchuyn gen 2 thng (05/2011)

2Xy dng quy trnh chuyn gen khng sutrn cy c chua .

3 thng (06/2011)

3nh gi cc tnh trng di truynca ccdng c chua chuyn gen 6 thng (07/2011-02/2012)

4Ti sinh cy chuyn gen trong iukin invitro.

6 thng (03/2012 - 08/2012)

5

Thu nhn v kim tra khnng khng su

ca cy chuyn gen trong iu kin invitro

3 thng (09/2012 - 11/2012)

6nh gi khnng khng ca cy chuyngen

6 thng (12/2012-04/2013)

7 Vit bo co nghim thu ti 1 thng 05/2013

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2.6. Dton kinh ph

Thi gian thchin ti: 2 nm (5/2011-5/2013)

Kinh phdkin: 210.000.000 Cng lao ng: 100.000.000 Nguyn vtliu: 400.000.000 Chi ph khc: 150.000.000

II. CNG CHI TT C TH

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7. Ti liu tham kho

TI LIUTINGVIT1. T Thu Cc, 2007. Gio trnh cy rau. Nh xutbn nng nghip, HNi.2. TrnQuc Dung, 2006. Cng nghchuyn gen ngvt,thcvt. Nh xutbnHu.3. Trnhnht, 2006. Cng ngh sinh hc, Tp 4, Cng ngh di truyn. Nh

xutbn gio dc, HNi.4.NguyncLng, 2006. Cng nghtbo. Nh xutbnihcquc gia tp.H Ch Minh.TI LIUTING ANH5. Hong-Xia Zhang v Eduardo Blumwald, 2001. Transgenic salt-tolerant tomatoplants accumulate salt in folige but not in fruit,Nature biotechnology, Vol.19. 765-768.6. Manoj K Sharma, 2009. A simple and efficient Agrobacterium-mediatedprocedure for transformation of tomato. Department of Plant Molecular Biology,University of Delhi South Campus, Benito Juarez Road, New Delhi 110 021,India.

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Đề cương nghiên cứu khoa học ( nhóm 2)