輸血部藤井明美 - UMINsquare.umin.ac.jp/.../qcws/file/qcws/16qcws/16QC_DNA_SSP.pdf輸血関連臓器移植造血幹細胞移植 SSP単独 SSP+ SSO,SBT 2 0 9 2 0 0 3 0 0

県立広島病院輸血部

藤井明美

テーマ

• 正確なDNAタイピングができること，第2区域まで判定されること

• DNAタイピング結果を正しく記述できること

• 学会の表記法に従い正確に表記すること

• DNAタイピング結果に対応したHLA抗原型を正確に読み替えること

• Ambiguityとなるアリルと日本人集団でのアリルの解説

試料（DNAsample）

• 日本人由来の細胞で高頻度に検出されるHLA型であること

• 日本人由来で稀なHLAアリルであること

• SSP法用の検体（DNA量；50～100ng/μl,200μl）配布

a b c ｄ

1 24D06メーカー

SSPは補助試薬

2 24D08 L-1 ○ ○

3 24D09 H-1 検査センター

4 24D10 ○ L-4 ○

5 24D11 ○ L-1 ○

6 24D13 L-1 メーカー

7 24D14 ○ L-2 ○

8 24D17 ○ L-1 ○ ○ ○

9 24D18 L-1,H-1 ○ ○ ○

10 24D20 ○ L-1,H-1 ○

11 24D21 ○ L-1,H-1 ○ ○ ○

12 24D22 ○ L-1 ○

13 24D23 ○ L-1 ○

14 24D25 L-1 ○

15 24D27 ○ L-1 ○

16 24D29 ○ L-1 ○ ○

17 24D31 ○ L-1 ○

18 24D36 L-1,L-2 ○ ○ ○

19 24D37 ○ L-2 ○

20 24D38 H-1,H-2,H-3 ○

参加部門

輸血関連

造血幹移植

その他

臓器移植

No.DNA-QC

Lab. No.

SSP単独

使用試薬

使用試薬名

•Low resolution

L-1 MocroSSPJPN

L-2 MicroSSPABDR

L-3 MicroSSP1L/2L/1A/1B/1C

L-4 AllsetGold

•High resolution

H-1 MicroSSPAlleleSpecific

H-2 AllsetGold

H-3 Unitray

a b c ｄ

21 24D39 ○ L-1 ○ ○

22 24D41 ○ L-1,L-3 ○

23 24D42 ○ L-1 ○ ○

24 24D43 ○ L-3 検査センター

25 24D47 ○ ○ SSPは補助試薬

26 24D49 ○ L-1 ○

27 24D51 ○ L-1 ○

参加部門

輸血関連

臓器移植

造血幹移植

その他

No.DNA-QC

Lab. No.

SSP単独

使用試薬

輸血関連臓器移植造血幹細胞移植

SSP単独 SSP+

SSO,SBT

○ 2 0

○ 9 2

○ 0 0

○ ○ 3 0

○ ○ 0 0

○ ○ 0 1

○ ○ ○ 2 2

その他（メーカー，検査センター） 1 2

SSP単独

18施設

Low resolution

ONELAMBDA MicroSSP JPN 14施設

ONELAMBDA MicroSSP ABDR 2施設

ONELAMBDA MicroSSP 1L/2L 2施設

Invitrogen AllsetGold 1施設

High resolution

ONELAMBDA MicroSSP Allele Specific

2施設

SSP+SSO,SBT

７施設

Low resolution

ONE LAMBDA MicroSSP JPN 5施設

ONE LAMBDA MicroSSP 1A/1B/1C/2L

1施設

High resolution

ONE LAMBDA MicroSSP Allele Specific

3施設

Invitrogen AllsetGold 1施設

Invitrogen Unitray 1施設

重複含む

low resolutionのタイピング法

（HLA抗原（型）レベルの結果を目的）

第1区域でアリル表記するものとする．

high resolutionのタイピング法

アリル表記は第2区域が必要．

判定結果

試験

検査状況

結果の

表記

Lab.ID HLA-A HLA-B HLA-C メーカー名キット名

24D08 A*02:01/02/03/+ A*24:/02/03/04/+ B*52:01/05/06/+ B*56:01/02/04/+ C*01:02/03/04/+ C*12:02/03/04/+ ワンラムダ社マイクロSSPJPN

24D10 A*02:03/25/38/+ A*24:02/04/05/+ invitrogen Allset Gold SSP HLA-A Low Res SSP Kit

24D10-2 B*52:01/04/05/+ B*56:01/16/19/+ invitrogen Allset Gold SSP HLA-B Low Res SSP Kit

24D11 A*02:01/02/03/+ A*24:02/03/04/+ B*52:01/05/06/+ B*56:01/02/04/+ One Lambda MicroSSP JPN

24D14 A*02:01/02/03/+ A*24:02/03/04/+ B*52:01/05/06/+ B*56:01/02/04/+ One Lambda Micro SSP ABDR

24D17 A*02:01/02/03/+ A*24:02/03/04/+ B*52:01/05/06/+ B*56:01/02/04/+ C*01:02/03/04/+ C*12:02/03/04/+ ワンラムダ Micro SSP JPN

24D18 A*02:03/230/253/+ A*24:02/98/101/+ B*52:01 B*56:01/08/14/+ C*01:02/25/26/+ C*12:02/22/27/+ Onelambda

Micro SSPJPN Micro SSP1A2

Micro SSPR1-24 Micro SSPR1-05 Micro SSP1-22

Micro SSPR1-C01 Micro SSPR1-C12

24D20 A*02:01/01L/02/+ A*24:02/02L/03/+ B*52:01/05/06/+ B*56:01/02/04/+ C*01:02/03/04/+ C*12:02/03/04/+ One Lambda, Inc. Micro SSPTM Japanese HLA

Class I and II ABCDRDQ DNA Typing Tray

24D20-2 A*02:03/135/171/+ - One Lambda, Inc. Micro SSPTM Allele Specific HLA Class I DNA Typing Tray - A*02


24D20-4 B*52:01 B*56:01/07/08/+ One Lambda, Inc. Micro SSPTM Allele Specific HLA Class I DNA Typing Tray - B*51/52/78

24D20-5 B*52:01/02/04/+ B*56:01/08/14/+ One Lambda, Inc. Micro SSPTM Allele Specific HLA Class I DNA Typing Tray - B*54-56

24D20-6 C*01:02/25/26/+ - One Lambda, Inc. Micro SSPTM Allele Specific HLA Class I Typing Tray - C*01

24D20-7 C*01:02/03/06/+ C*12:02/22/27/+ One Lambda, Inc. Micro SSPTM Allele Specific HLA Class I Typing Tray - C*12

24D21 A*02:01/02/03/+ A*24:02/03/04/+ B*52:01/05/06/+ B*56:01/02/04/+ C*01:02/03/04/+ C*12:02/03/04/+ One Lambda MicroSSP JPN ABCDRDQ

24D22 A*02:01//+ A*24:02//+ B*52:01//+ B*56:01/02/04/+ One Lambda Micro SSP Japanese HLA Class Ⅰ and Ⅱ ABCDRDQ DNA Typing Tray

24D23 A*02:01/02/03/+ A*24:02/03/04/+ B*52:01/05/06/+ B*56:01/02/04/+ C*01:02/03/04/+ C*12:02/03/04/+ One Lambda Micro SSP HLADNAﾀｲﾋﾟﾝｸﾞｷｯﾄ

24D25 A*02:01/02/03/+ A*24:02/03/04/+ B*52:01/05/06/+ B*56:01/02/04/+ C*01:02/03/04/+ C*12:02/03/04/+ ONE LAMBDA Micro SSP-JPN

24D27 A*02:01/02/03/+ A*24:02/03/04/+ B*52:01/05/06/+ B*56:01/02/04/+ C*01:02/03/04/+ C*12:02/03/04/+ ONE LAMBDA Micro SSP JPN

24D29 A*02:01/02/03/+ A*24:02/03/04/+ B*52:01/05/06/+ B*56:01/02/08/+ C*01:02/03/05/+ C*12:02/03/04/+ One Lambda Micro SSP DNA Typing Tray

24D31 A*02:01//+ A*24:02//+ B*49:03/52:01/+ B*56:01/02/04/+ C*01:01//+ C*01:01//+ One Lambda MicroSSP Japanese HLA ClassⅠandⅡABCDRDQ DNA Typing Tray

24D36 A*02:03/171/230/+ A*24:02/04/05/+ B*49/52 B*56 C*01 C*01/08/12/+ One Lamda

Micro SSP JPN Micro SSP 1A

Micro SSP 1A2 Micro SSP 1B Micro SSP 1C

24D37 A*02:01/02/03/+ A*24:02/03/04/+ B*52:01/05/06/+ B*56:01/02/04/+ One Lambda MicroSSP ABDR

24D38 実施なし実施なし B*52:01 B*56:01/08/14/+ C*01:02/25/26/+ C*12:02/22/27/+

Invitrogen /One Lambda

B*52SSP Unitray Micro SSP B*54-56

Micro SSP C*01 Micro SSP C*12

24D39 A*02:01/03/06/+ A*24:02/04/08/+ B*56:07 B*78:06 C*01:02/03 C*12:02/03 ベリタス Micro SSP

24D41 A*02:01/02/03/+ A*24:02/03/04/+ B*52:01/05/06/+ B*56:01/02/04/+ C*01:02/03/07/+ C*12:02/14/16/+ べりタス SSP 1L

24D42 A*02:01/02/03/+ A*24:02/03/04/+ B*52:01/05/06/+ B*56:01/08/13/+ C*01:02/03/05/+ - ワンラムダ Micro SSP JPN

24D43 A*02:01/02/03/+ A*24:02/03/04/+ B*52:01/05/06/+ B*56:01/02/04/+ C*01:02/03/07/+ C*12:02/14/16/+ ONE LAMBDA SSP1L

24D49 A*02:01/02/03/+ A*24:02/03/04/+ B*52:01/05/06/+ B*56:01/02/08/+ C*01:02/03/05/+ C*12::02/18/22/+ ONELAMBDA Micoro SSP DNA typing Trars SSP JPN

24D51 A*02:01/02/03/+ A*24:02/03/04/+ B*52:01/05/06/+ B*56:01/02/04/+ C*01:02/03/04/+ C*12:02/03/04/+ One Lambda MicroSSP JPN ABC/DRDQ


24D08 A*11:01/02/03/+ A*31:01/02/03/+ B*46:01/02/04/+ - C*01:02/03/05/+ - ワンラムダ社マイクロSSPJPN


24D10-2 B*46:01/02/03/+ - invitrogen Allset Gold SSP HLA-B Low Res SSP Kit

24D11 A*11:01/02/03/+ A*31:01/02/03/+ B*46:01/02/04/+ - One Lambda MicroSSP JPN

24D14 A*11:01/02/03/+ A*31:01/02/03/+ B*46:01/02/05/+ - One Lambda Micro SSP ABDR

24D17 A*11:01/02/03/+ A*31:01/02/03/+ B*46:01/02/04/+ - C*01:02/03/05/+ - ワンラムダ Micro SSP JPN

24D18 A*11:01/65/66/+ A31:01/18/25/+ B*46:01/20/22/+ - C*01:02/25/26/+ - Onelambda

Micro SSPJPN Micro SSPR1-11 Micro SSPR1-31 Micro SSPR1-46

Micro SSPR1-C01

24D20 A*11:01/02/03/+ A*31:01/02/03/+ B*46:01/02/04/+ - C*01:02/03/05/+ - One Lambda, Inc. Micro SSPTM Japanese HLA


24D20-2 A*11:01/02/16/+ - One Lambda, Inc. Micro SSPTM Allele Specific HLA Class I Typing Tray - A*11


24D20-4 B*46:01/02/03/+ - One Lambda, Inc. Micro SSPTM HLA Class I B Locus Specific DNA Typing Tray

24D20-5 B*46:01/03/05/+ - One Lambda, Inc. Micro SSPTM Allele Specific HLA Class I DNA Typing Tray - B*46/67/81/82

24D20-6 C*01:02/03/05/+ - One Lambda, Inc. Micro SSPTM HLA Class I C Locus Specific DNA Typing Tray


24D21 A*11:01/02/03/+ A*31:01/02/03/+ B*46:01/02/04/+ - C*01:02/03/05/+ - One Lambda MicroSSP JPN ABCDRDQ

24D22 A*11:01//+ A*31:01/02/03/+ B*46:01/02/04/+ undefined One Lambda Micro SSP Japanese HLA Class Ⅰ and Ⅱ ABCDRDQ DNA Typing Tray

24D23 A*11:01/02/03/+ A*31:01/02/03/+ B*46:01/02/04/+ - C*01:02/03/05/+ - One Lambda Micro SSP HLADNAﾀｲﾋﾟﾝｸﾞｷｯﾄ

24D25 A*11:01/02/03/+ A*31:01/02/03/+ B*46:01/02/04/+ - C*01:02/03/05/+ - ONE LAMBDA Micro SSP-JPN

24D27 A*11:01/02/03/+ A*31:01/02/03/+ B*46:01/02/05/+ - C*01:02/03/05/+ - ONE LAMBDA Micro SSP JPN

24D29 A*11:01/02/03/+ A*31:01/02/04/+ B*46:01/02/05/+ - C*01:02/03/05/+ - One Lambda Micro SSP DNA Typing Tray

24D31 A*03:88/11:01/+ A*31:01/02/03/+ B*15:143/27:26/+ B*46:01/02/04/+ C*01:02/03/05/+ - One Lambda MicroSSP Japanese HLA ClassⅠandⅡABCDRDQ DNA Typing Tray

24D36 A*11:01/02/03/+ A*31:01/02/03/+ B*15/35/46 B*46 C*01 - One Lamda

Micro SSP JPN Micro SSP 1A Micro SSP 1B Micro SSP 1C

24D37 A*11:01/02/03/+ A*31:01/02/03/+ B*46:01/02/03/+ - One Lambda MicroSSP ABDR

24D38 A*11:01/29/30/+ A*31:01/15/16/+ B*46:01/20/22/+ 実施なし C*01:02/25/26/+ 実施なし

Invitrogen Invitrogen

One Lambda One Lambda

A*11SSP Unitray A*19SSP Unitray

Micro SSP B*46/67/81/82 Micro SSP C*01

24D39 A*11:01/02 A*31:01/05 B*46:01/02 ― C*01:02/03 ― ベリタス Micro SSP

24D41 A*11:01/02/03/+ A*31:01/02/03/+ B*46:01/02/04/+ - C*01:02/03/05/+ - べりタス SSP JPN

24D42 A*11:01/02/03/+ A*31:01/02/04/+ B*46:01/02/05/+ - C*01:02/03/05/+ - ワンラムダ Micro SSP JPN

24D43 A*11:01/02/03/+ A*31:01/02/03/+ B*46:01/02/03/+ - C*01:02/03/05/+ - ONE LAMBDA SSP1L

24D49 A*11:01//+ A*31:01//+ B*46:01/05/07/+ - C*01:02/03/05/+ - ONELAMBDA Micoro SSP DNA typing Trars SSP JPN

24D51 A*11:01/02/03/+ A*31:01/02/03/+ B*46:01/02/05/+ - C*01:02/03/05/+ - One Lambda MicroSSP JPN ABC/DRDQ


24D08 A*02:01/02/03/+ A*11:01/02/03/* B*38:01/02/04/+ B*48:01/06/07/+ C*07:01/02/03/+ C*08:01/02/03/+ ワンラムダ社マイクロSSPJPN






24D18 A*02:06/126/127/+ A*11:02 B*38:02/08/15/+ B*48:01 C*07:02/32/56/+ C*08:03/38/40 Onelambda


Micro SSPR1-11 Micro SSP1-16

Micro SSPR1-48 Micro SSPR1-C07 Micro SSPR1-C08

24D20 A*02:01/01L/02/+ A*11:01/02/03/+ B*38:01/02/04/+ B*48:01/06/07/+ C*07:01/02/03/+ C*08:01/02/03/+ One Lambda, Inc. Micro SSPTM Japanese HLA



24D20-3 A*11:02/16/38/+ - One Lambda, Inc. Micro SSPTM Allele Specific HLA Class I Typing Tray - A*11

24D20-4 B*38:02/08/15/+ B*48:01/03/04/+ One Lambda, Inc. Micro SSPTM Allele Specific HLA Class I DNA Typing Tray - B*38/39

24D20-5 B*48:01/20/21/+ - One Lambda, Inc. Micro SSPTM Allele Specific HLA Class I DNA Typing Tray - B*48

24D20-6 C*07:02/32N/41/+ - One Lambda, Inc. Micro SSPTM Allele Specific HLA Class I Typing Tray - C*07



24D22 A*02:01//+ A*11:01//+ B*38:01/02/04/+ B*48:01//+ One Lambda Micro SSP Japanese HLA Class Ⅰ and Ⅱ ABCDRDQ DNA Typing Tray


24D25 A*02:01/02/03/+ A*11:01/02/03/+. B*38:01/02/04/+ B*48:01/06/07/+ C*07:01/02/03/+ C*08:01/02/03/+ ONE LAMBDA Micro SSP-JPN


24D29 A*02:01/02/03/+ A*11:01/02/03/+ B*38:01/02/08/+ B*48:01/07/09/+ C*07:01/02/03/+ C*08:01/02/03/+ One Lambda Micro SSP DNA Typing Tray

24D31 A*02:01//+ A*03:88//+ B*38:01/02/04/+ B*48:01/81:05/+ C*07:01//+ C*07:101//+ One Lambda MicroSSP Japanese HLA ClassⅠandⅡABCDRDQ DNA Typing Tray

24D36 A*02:06/116/126/+ A*11:01/02/03/+ B*38 B*48/81 C*07 C*08 One Lamda




24D38 A*02:06/126/170/+ A*11:02/40/45/+ B*38:02/08/15/+ 実施なし C*07:02/56/75/+ C*08:03/38/40

Invitrogen Invitrogen Invitrogen

One Lambda One Lambda

Ａ*02SSP Unitray A*19SSP Unitray Ｂ*38SSP Unitray

Micro SSP C*07 Micro SSP C*08

24D39 A*02:01/03/06/+ A*11:01/02 B*38:02 B*48:01 C*07:02/04 C*08:01/03 ベリタス Micro SSP

24D41 A*02:01/02/03/+ A*11:01/02/03/+ B*38:01/02/08/+ B*48:01/07/09/+ C*07:01/02/03/+ C*08:01/02/03/+ べりタス SSP JPN


24D43 A*02:01/02/03/+ A*11:01/02/03/+ B*38:01/02/04/+ B*48:01/06/09/+ C*07:01/02/05/+ C*08:01/02/03/+ ONE LAMBDA SSP1L

24D49 A*02:01/02/03/+ A*11/34 B*38:01/08/09/+ B*48:01/07/09/+ C*07:01/02/03/+ C*08:01/02/03/+ ONELAMBDA Micoro SSP DNA typing Trars SSP JPN



24D06 MBL ジェノサーチサポートSSP「B5」

24D08 A*02:01/02/03/+ A*24:/02/03/04/+ B*15:05/30/39/+ B*51:01/03/07/+ C*07:01/02/03/+ C*14:02/05/06/+ ワンラムダ社マイクロSSPJPN




24D13 A*02/24 undefined B*15 B*51 C*07 C*06/14 OneLambda マイクロSSPA,B,C,DRB,DQB1



24D18 A*02:01/97/105/+ A*24:02/98/101/+ B*15:05 B*51:01/96/105 C*07:02/56/75/+ C*14:02/11/14/+ Onelambda


Micro SSPR1-24 Micro SSPR1-15 Micro SSPR1-05

Micro SSPR1-C07 Micro SSPR1-C14

24D20 A*02:01/01L/02/+ A*24:02/02L/03/+ B*15:05/30/39/+ B*51:01/03/07/+ C*07:01/02/03/+ C*14:02/05/06/+ One Lambda, Inc. Micro SSPTM Japanese HLA




24D20-4 B*15:05/155 B*51:01/02/03/+ One Lambda, Inc. Micro SSPTM Allele Specific HLA Class I DNA Typing Tray - B*15

24D20-5 B*15:01/01N/05/+ B*51:01/94/95/+ One Lambda, Inc. Micro SSPTM Allele Specific HLA Class I DNA Typing Tray - B*51/52/78




24D22 A*02:01//+ A*24:02//+ B*15:05/30/39/+ B*51:01/03/07/+ One Lambda Micro SSP Japanese HLA Class Ⅰ and Ⅱ ABCDRDQ DNA Typing Tray


24D25 A*02:01/02/03/+ A*24:02/03/04/+ B*15:05/30/39/+ B*51:01/03/07/+ C*07:01/02/03/+ C*14:02/05/06/+ ONE LAMBDA Micro SSP-JPN


24D29 A*02:01/02/03/+ A*24:02/03/04/+ B*15:05/39/155/+ B*51:01/03/07/+ C*07:01/02/03/+ C*14:02*05*06/+ One Lambda Micro SSP DNA Typing Tray

24D31 A*02:01//+ A*24:02//+ B*15:05/30/39/+ B*51:01/03/07/+ C*06:11//+ C*07:64//+ One Lambda MicroSSP Japanese HLA ClassⅠandⅡABCDRDQ DNA Typing Tray

24D36 A*02:01/06/97/+ A*24/02/04/05/+ B*15 B*51 C*07 C*14 One Lamda




24D38 実施なし実施なし B*51:01 実施なし C*14:02/11/13/+ C*07:02/56/75/+

Invitrogen One Lambda One Lambda

B*51SSP Unitray Micro SSP C*07 Micro SSP C*14

24D39 A*02:01/03/06/+ A*24:02/04/08/+ B*15:05/30/39/+ B*51:01/03 C*07:02 C*14:02 ベリタス Micro SSP

24D41 A*02:01/02/03/+ A*24:02/03/04/+ B*15:05/30/39/+ B*51:01/03/07/+ C*07:01/02/03/+ C*14:02/05/06/+ べりタス SSP JPN

24D42 A*02:01/02/03/+ A*24:02/03/04/+ B*51:01/03/07+ B15:05/39/155/+ C*07:01/02/03/+ C*14*02/05/06/+ ワンラムダ Micro SSP JPN

24D43 A*02:01/02/03/+ A:24:02/03/04/+ B*51:01/03/07/+ B*15:05/39/107/+ C*07:01/02/05/+ C*14:02/04/05/+ ONE LAMBDA SSP1L

24D49 A*02:01/02/03/+ A*24:02/03/04/+ B*15:05/39/40/+ B*51:01/03/07/+ C*07:01/02/05/+ C*14:02/05/07/+ ONELAMBDA Micoro SSP DNA typing Trars SSP JPN


Lab.ID HLA-DRB1 HLA-DRB3/4/5 HLA-DQB1 メーカー名キット名

24D08-2 DRB1*13:01/02/03/+ DRB1*15:01/02/03/+ DRB3*01//+ DRB5*01/02 DQB1*03:01/04/09/+ DQB1*06:01/02/06/+ ワンラムダ社マイクロSSPJPN

24D09 invitrogen Allset Gold SSP HLA-DQA1 High Res Kit

24D10-3 DRB1*13:04/07/12/+ DRB1*15:01/02/03/+ DRB3*01/02/03 DRB5*01/02 invitrogen Allset Gold SSP HLA-DR Low Res SSP Kit

24D11-2 DRB1*13:01/02/03/+ DRB1*15:01/02/03/+ One Lambda MicroSSP JPN

24D14-2 DRB1*13:01/02/03/+ DRB1*15:01/02/03/+ DRB3*01/02/03 DRB5*01/02 One Lambda Micro SSP ABDR

24D17-2 DRB1*13:01/02/03/+ DRB1*15:01/02/03/+ DRB3*01/02/03 DRB5*01/02 DQB1*03:01/04/09/+ DQB1*06:01/02/03/+ ワンラムダ Micro SSP JPN

24D18-2 DRB1*13:12 DRB1*15:02/44/47/+ DRB3*01/02/03 DRB5*01/02 DQB1*03:01/10/21/+ DQB1*06:01/35 Onelambda

Micro SSPJPN Micro SSPR2-113 Micro SSPR2-115 Micro SSPR2-Q1

24D20-8 DRB1*13:01/02/03/+ DRB1*15:01/02/03/+ DRB3*01/02/03 DRB5*01/02 DQB1*03:01/04/09/+ DQB1*06:01/02/03/+ One Lambda, Inc. Micro SSPTM Japanese HLA


24D20-9 DRB1*13:12 DRB1*15:02/08/14/+ One Lambda, Inc. Micro SSPTM Allele Specific HLA Class II DNA Typing Tray - DRB1*13

24D20-10 DRB1*15:02/26/27/+ - One Lambda, Inc. Micro SSPTM Allele Specific HLA Class II DNA Typing Tray - DRB1*15

24D20-11 DQB1*03:01/10/21/+ DQB1*06:01/35 One Lambda, Inc. Micro SSPTM Allele Specific HLA Class II DNA Typing Tray - DQB1

24D21-2 DRB1*13:01/02/03/+ DRB1*15:01/02/03/+ DRB3*01/02/03 DRB5*01/02 DQB1*03:01/04/09/+ DQB1*06:01/02/03/+ One Lambda MicroSSP JPN ABCDRDQ

24D21-3

24D22-2 DRB1*13:01//+ DRB1*15:01/02/03/+ DRB3* DRB5* One Lambda Micro SSP Japanese HLA Class Ⅰ and Ⅱ ABCDRDQ DNA Typing Tray

24D23-2 DRB1*13:01/02/03/+ DRB1*15:01/02/03/+ DRB3*01:01/02/03/+ DRB5*01:01/02/03/+ DQB1*03:01/04/09/+ DQB1*06:01/02/03/+ One Lambda Micro SSP HLADNAﾀｲﾋﾟﾝｸﾞｷｯﾄ

24D25-2 DRB1*13:01/02/03/+ DRB1*15:01/02/03/+ DRB3*01/02/03 DRB5*01/02 DQB1*03:01/04/09/+ DQB1*06:01/02/03/+ ONE LAMBDA Micro SSP-JPN

24D27-2 DRB1*13:01/02/03/+ DRB1*15:01/02/03/+ DQB1*03:01/04/09/+ DQB1*06:01/02/03/+ ONE LAMBDA Micro SSP JPN

24D29-2 DRB1*13:01/02/03/+ DRB1*15:01/02/03/+ DRB3*01/02/03 DRB5*01/02 DQB1*03:01/04/09/+ DQB1*06:01/02/03/+ One Lambda Micro SSP DNA Typing Tray

24D31-2 DRB1*13:01/14:22/+ DRB1*15:01/02/03/+ DRB3*01:01//+ DRB5*01:01//+ DQB1*03:01/04/09/+ DQB1*06:01/02/03/+ One Lambda MicroSSP Japanese HLA ClassⅠandⅡABCDRDQ DNA Typing Tray

24D36-2 DRB1*13/14 DRB1*15 DRB3*01//+ DRB5*01//+ DQB1*03 DQB1*06 One Lamda Micro SSP JPN Micro SSP 2L

24D37-2 DRB1*13:01/02/03/+ DRB1*15:01/02/03/+ DRB3*01/02/03 DRB5*01/02 One Lambda MicroSSP ABDR

24D38-2 DRB1*13:12/118 実施なし実施なし

実施なし

DQB1*03:01/21/22/+ DQB1*06:01

Invitrogen

Allset Gold SSP HLA-DRB1*13 Allset Gold SSP HLA-DQB1*03 Allset Gold SSP HLA-DQB1*06

DPB1 SSP Unitray

24D39-2 DRB1*13:01/02/07 DRB1*15:01/02 DRB3*01:01/02：02/03：01 DRB5＊01：01/02 DQB1*03:01 DQB1*06:01/02/03/+ ベリタス Micro SSP

24D41-2 DRB1*13:01/02/03/+ DRB1*15:01/02/03/+ DRB3*01/02/03 DRB5*01/02 DQB1*03:01/04/09/+ DQB1*06:01/02/03/+ べりタス SSP JPN

24D42-2 DRB1*13:01/02/03/+ DRB1*15:01/02/03/+ DRB5*01/02 DRB3*01//+ DQB1*06:01/02/03/+ DQB1*03:01/04/09/+ ワンラムダ Micro SSP JPN

24D43-2 DRB1*13:01/02/03/+ DRB1*15:01/02/03/+ DRB3*01/02/03 DRB5*01/02 DQB1*03:01/04/09/+ DQB1*06:01/02/03/+ ONE LAMBDA SSP2L

24D49-2 DRB1*13:01/02/03/+ DRB1*15:01/02/03/+ DRB3*01:01/02:01/+ DRB5*01:01/02:02/+ DQB1*03:01/02/03/+ DQB1*06:01/02/03/+ ONELAMBDA Micoro SSP DNA typing Trars SSP JPN

24D51-2 DRB1*13:01/02/03/+ DRB1*15:01/02/03/+ DRB3*01/02/03 DRB5*01/02 DQB1*03:01/04/09/+ DQB1*06:01/02/03/+ One Lambda MicroSSP JPN ABC/DRDQ


24D08-2 DRB1*09:01/02/03/+ DRB1*12:01/02/03/+ DRB4*01 DRB3*01//+ DQB1*03:01/04/09/+ DQB1*03:03/06/12/+ ワンラムダ社マイクロSSPJPN


24D10-3 DRB1*09:01/02/03/+ DRB1*12:01/02/03/+ DRB3*01/02/03 DRB4*01:01/02/03/+ invitrogen Allset Gold SSP HLA-DR Low Res SSP Kit


24D14-2 DRB1*09:01/02/03/+ DRB1*12:01/02/03/+ DRB3*01/02/03 DRB4*01:01/02/03/+ One Lambda Micro SSP ABDR

24D17-2 DRB1*09:01/02/03/+ DRB1*12:01/02/03/+ DRB3*01/02/03 DRB4*01 DQB1*03:01/04/09/+ DQB1*03:03/06/12/+ ワンラムダ Micro SSP JPN

24D18-2 DRB1*09:01/11/12/+ DRB1*12:01/25/28/+ DRB4*01:01/02/03/+ DRB3*01/02/03 DQB1*03:01/21/22/+ DQB1*03:03/30/31/+ Onelambda


24D20-8 DRB1*09:01/02/03/+ DRB1*12:01/02/03/+ DRB3*01/02/03 DRB4*01:01/02/03/+ DQB1*03:01/04/09/+ DQB1*03:03/06/12/+ One Lambda, Inc. Micro SSPTM Japanese HLA


24D20-9 DRB1*09:01/02/06/+ - One Lambda, Inc. Micro SSPTM Allele Specific HLA Class II DNA Typing Tray - DRB1*07/09/10


24D20-11 DQB1*03:01/21/22/+ DQB1*03:03/30/31/+ One Lambda, Inc. Micro SSPTM Allele Specific HLA Class II DNA Typing Tray - DQB1

24D21-2 DRB1*09:01/02/03/+ DRB1*12:01/02/03/+ DRB3*01/02/03 DRB4*01 DQB1*03:01/04/09/+ DQB1*03:03/06/12/+ One Lambda MicroSSP JPN ABCDRDQ

24D21-3

24D22-2 DRB1*09:01/02/03/+ DRB1*12:01/02/03/+ DRB3* DRB4* One Lambda Micro SSP Japanese HLA Class Ⅰ and Ⅱ ABCDRDQ DNA Typing Tray

24D23-2 DRB1*09:01/02/03/+ DRB1*12:01/02/03/+ DRB4*01:01/02/03/+ DRB3*01:01/02/03/+ DQB1*03:01/04/09/+ DQB1*03:03/06/12/+ One Lambda Micro SSP HLADNAﾀｲﾋﾟﾝｸﾞｷｯﾄ

24D25-2 DRB1*09:01/02/03/+ DRB1*12:01/02/03/+ DRB4*01:01/02/03/+ DRB3*01/02/03 DQB1*03:01/04/09/+ DQB1*03:03/06/12/+ ONE LAMBDA Micro SSP-JPN


24D29-2 DRB1*09:01/02/03/+ DRB1*12:01/02/03/+ DRB3*01/02/03 DRB4*01:01/02/03/+ DQB1*03:01/04/09/+ DQB1*03:03/06/12/+ One Lambda Micro SSP DNA Typing Tray

24D31-2 DRB1*09:01/02/03/+ DRB1*12:01/02/03/+ DRB3*01:01/02:01/+ DRB4*01:01/02/03/+ DQB1*03:01/04/09/+ DQB1*03:03/04:03/+ One Lambda MicroSSP Japanese HLA ClassⅠandⅡABCDRDQ DNA Typing Tray

24D36-2 DRB1*09 DRB1*12 DRB3*01/02/03 DRB4*01 DQB1*03 DQB1*03 One Lamda Micro SSP JPN Micro SSP 2L

24D37-2 DRB1*09:01/02/03/+ DRB1*12:01/02/03/+ DRB3*01/02/03 DRB4*01:01/02/03/+ One Lambda MicroSSP ABDR

24D38-2 DRB1*12:01 実施なし実施なし実施なし DQB1*03:01/21/22/+ DQB1*03:03/20 Invitrogen

Allset Gold SSP HLA-DRB1*08/12 Allset Gold SSP HLA-DQB1*03

DPB1 SSP Unitray

24D39-2 DRB1*09:01 DRB1*12:01/02 DRB4*01:03 DRB3*01:01/03:01 DQB1*03:03 DQB1*03:01 ベリタス Micro SSP

24D41-2 DRB1*09:01/02/03/+ DRB1*12:01/02/03/+ DRB3*01/02/03 DRB4*01 DQB1*03:01/04/09/+ DQB1*03:03/06/12/+ べりタス SSP JPN

24D42-2 DRB1*09:01/02/03/+ DRB1*12:01/02/03/+ DRB3*01//+ DRB4*01 DQB1*03:01/04/09/+ DQB1*03:03/06/12/+ ワンラムダ Micro SSP JPN

24D43-2 DRB1*09:01/02/03/+ DRB1*12:01/02/03/+ DRB3*01/02/03 DRB4*01:01/02/03/+ DQB1*03:01/04/09/+ DQB1*03:03/06/12/+ ONE LAMBDA SSP2L

24D49-2 DRB1*09:01/02/03/+ DRB1*12:01/02/03/+ DRB3*01:01/02:01/+ DRB4*01:01/02/03/+ DQB1*03:01/04/09/+ DQB1*03:03/06/12/+ ONELAMBDA Micoro SSP DNA typing Trars SSP JPN

24D51-2 DRB1*09:01/02/03/+ DRB1*12:01/02/03/+ DRB3*01/02/03 DRB4*01:01/02/03/+ DQB1*03:01/04/09/+ DQB1*03:03/06/12/+ One Lambda MicroSSP JPN ABC/DRDQ


24D08-2 DRB1*04:01/03/04/+ DRB1*16:01/02/03/+ DRB4*01 DRB5*01/02 DQB1*04:01/02/04/+ DQB1*05:01/02/03/+ ワンラムダ社マイクロSSPJPN


24D10-3 DRB1*04:01/02/03/+ DRB1*16:01/02/03/+ DRB4*01:01/02/03/+ DRB5*01/02 invitrogen Allset Gold SSP HLA-DR Low Res SSP Kit


24D14-2 DRB1*04:01/02/03/+ DRB1*16:01/02/03/+ DRB4*01:01/02/03/+ DRB5*01/02 One Lambda Micro SSP ABDR

24D17-2 DRB1*04:01/03/04/+ DRB1*16:01/02/03/+ DRB4*01 DRB5*01/02 DQB1*04:01/02/04/+ DQB1*05:01/02/03/+ ワンラムダ Micro SSP JPN

24D18-2 DRB1*04:01 DRB1*16:02/11/14 DRB4*01:01/02/03/+ DRB5*01/02 DQB1*04:02 DQB1*05:02 Onelambda


24D20-8 DRB1*04:01/03/04/+ DRB1*16:01/02/03/+ DRB4*01:01/02/03/+ DRB5*01/02 DQB1*04:01/02/04/+ DQB1*05:01/02/03/+ One Lambda, Inc. Micro SSPTM Japanese HLA


24D20-9 DRB1*04:10 - One Lambda, Inc. Micro SSPTM Allele Specific HLA Class II DNA Typing Tray - DRB1*04


24D20-11 DQB1*04:02 DQB1*05:02 One Lambda, Inc. Micro SSPTM Allele Specific HLA Class II DNA Typing Tray - DQB1

24D21-2 DRB1*04:01/03/04/+ DRB1*16:01/02/03/+ DRB4*01 DRB5*01/02 DQB1*04:01/02/04/+ DQB1*05:01/02/03/+ One Lambda MicroSSP JPN ABCDRDQ

24D21-3

24D22-2 DRB1*04:01/03/04/+ DRB1*16:01//+ DRB4* DRB5* One Lambda Micro SSP Japanese HLA Class Ⅰ and Ⅱ ABCDRDQ DNA Typing Tray

24D23-2 DRB1*04:01/03/04/+ DRB1*16:01/02/03/+ DRB4*01:01/02/03/+ DRB5*01/02 DQB1*04:01/02/04/+ DQB1*05:01/02/03/+ One Lambda Micro SSP HLADNAﾀｲﾋﾟﾝｸﾞｷｯﾄ

24D25-2 DRB1*04:01/03/04/+ DRB1*16:01/02/03/+ DRB4*01:01/02/03/+ DRB5*01/02 DQB1*04:01/02/04/+ DQB1*05:01/02/03/+ ONE LAMBDA Micro SSP-JPN

24D27-2 DRB1*04:01/03/04/+ DRB1*16:01/02/03/+ DQB1*04:01/02/04/ DQB1*05:01/02/03/+ ONE LAMBDA Micro SSP JPN

24D29-2 DRB1*04:01/03/04/+ DRB1*16:01/02/03/+ DRB4*01:01/02/03/+ DRB5*01/02 DQB1*04:01/02/04 DQB1*05:01/02/03/+ One Lambda Micro SSP DNA Typing Tray

24D31-2 DRB1*04:01/03/04/+ DRB1*15:34/16:01/+ DRB4*01:01/02/03/+ DRB5*01:01/02:02/+ DQB1*04:01/02/04/+ DQB1*05:01/02/03/+ One Lambda MicroSSP Japanese HLA ClassⅠandⅡABCDRDQ DNA Typing Tray

24D36-2 DRB1*04 DRB1*16 DRB4*01 DRB5*01/02 DQB1*04 DQB1*05 One Lamda Micro SSP JPN Micro SSP 2L

24D37-2 DRB1*04:01/02/03/+ DRB1*16:01/02/03/+ DRB4*01:01/02/03/+ DRB5*01/02 One Lambda MicroSSP ABDR

24D38-2 実施なし実施なし実施なし実施なし実施なし実施なし Invitrogen DPB1 SSP Unitray

24D39-2 DRB1*04:05/10 DRB1*16:02 DRB4*01:03 DRB5＊02：02 DQB1*04:01/02 DQB1*05:02 ベリタス Micro SSP

24D41-2 DRB1*04:01/03/04/+ DRB1*16:01/02/03/+ DRB4*01 DRB5*01/02 DQB1*04:01/02/04/+ DQB1*05:01/02/03/+ べりタス SSP JPN

24D42-2 DRB1*04:01/03/04/+ DRB1*16:01/02/03/+ DRB5*01/02 DRB4*01 DQB1*04:01/02/04/+ DQB1*05:01/02/03/+ ワンラムダ Micro SSP JPN

24D43-2 DRB1*04:01/02/03/+ DRB1*16:01/02/03/+ DRB4*01:01/02/03/+ DRB5*01/02 DQB1*04:01/02/04/+ DQB1*05:01/02/03/+ ONE LAMBDA SSP2L

24D49-2 DRB1*04:01//+ DRB1*15:34//+ DRB4*01:01/02/03/+ DRB5*01:01/02:02/+ DQB1*04:03 DQB1*05:01/02/03/+ ONELAMBDA Micoro SSP DNA typing Trars SSP JPN

24D51-2 DRB1*04:01/03/04/+ DRB1*16:01/02/03/+ DRB4*01:01/02/03/+ DRB5*01/02 DQB1*04:01/02/04/+ DQB1*05:01/02/03/+ One Lambda MicroSSP JPN ABC/DRDQ


24D08-2 DRB1*08:02/04/09/+ DRB1*14:01/02/05/+ DRB3*01//+ - DQB1*03:01/04/09/+ DQB1*04:01/02/04/+ ワンラムダ社マイクロSSPJPN


24D10-3 DRB1*08:01/02/03/+ DRB1*14:02/03/06/+ DRB3*01/02/03 - invitrogen Allset Gold SSP HLA-DR Low Res SSP Kit


24D13-2 DRB1*08/13/14 undefined DQB1*03:01/04/09/+ DQB1*04:01/02/04 OneLambda マイクロSSPA,B,C,DRB,DQB1

24D14-2 DRB1*08:01/02/03/+ DRB1*14:02/03/05/+ DRB3*01/02/03 - One Lambda Micro SSP ABDR

24D17-2 DRB1*08:02/04/13/+ DRB1*14:01/02/05/+ DRB3*01/02/03 - DQB1*03:01/04/09/+ DQB1*04:01/02/04/+ ワンラムダ Micro SSP JPN

24D18-2 DRB1*08:02 DRB1*14:06 - DRB3*01/02/03 DQB1*03:01/21/22/+ DQB1*04:02 Onelambda


24D20-8 DRB1*08:02/04/09/+ DRB1*14:01/02/05/+ DRB3*01/02/03 - DQB1*03:01/04/09/+ DQB1*04:01/02/04/+ One Lambda, Inc. Micro SSPTM Japanese HLA


24D20-9 DRB1*08:02/42 - One Lambda, Inc. Micro SSPTM Allele Specific HLA Class II DNA Typing Tray - DRB1*08

24D20-10 DRB1*14:06/83/108 - One Lambda, Inc. Micro SSPTM Allele Specific HLA Class II DNA Typing Tray - DRB1*14

24D20-11 DQB1*03:01/21/22/+ DQB1*04:02 One Lambda, Inc. Micro SSPTM Allele Specific HLA Class II DNA Typing Tray - DQB1

24D21-2 DRB1*08:02/04/13/+ DRB1*14:01/02/05/+ DRB3*01/02/03 - DQB1*03:01/04/09/+ DQB1*04:01/02/04/+ One Lambda MicroSSP JPN ABCDRDQ

24D21-3 DRB1*08:02 DRB1*14:06/108 DRB3*02:02/23/25 - One Lambda MicroSSP 2H

24D22-2 DRB1*08:02//+ DRB1*14:01//+ DRB3* - One Lambda Micro SSP Japanese HLA Class Ⅰ and Ⅱ ABCDRDQ DNA Typing Tray

24D23-2 DRB1*08:02/04/09/+ DRB1*14:01/02/05/+ DRB3*01:01/02/03/+ - DQB1*03:01/04/09/+ DQB1*04:01/02/04/+ One Lambda Micro SSP HLADNAﾀｲﾋﾟﾝｸﾞｷｯﾄ

24D25-2 DRB1*08:02/04/09/+ DRB1*14:01/02/05/+ DRB3*01/02/03 - DQB1*03:01/04/09+ DQB1*04:01/02/04/+ ONE LAMBDA Micro SSP-JPN


24D29-2 DRB1*08:02/04/13/+ DRB1*14:01/02/05/+ DRB3*01/02/03 - DQB1*03::01/04/09/+ DQB1*04/01/02/04 One Lambda Micro SSP DNA Typing Tray

24D31-2 DRB1*03:17//+ DRB1*08:02//+ DRB3*01:01//+ DRB3*01:01//+ DQB1*03:01/04/09/+ DQB1*04:01/02/04/+ One Lambda MicroSSP Japanese HLA ClassⅠandⅡABCDRDQ DNA Typing Tray

24D36-2 DRB1*08 DRB1*14 DRB3*01/02 DQB1*03 DQB1*04 One Lamda Micro SSP JPN Micro SSP 2L

24D37-2 DRB1*08:01/02/03/+ DRB1*14:02/03/05/+ DRB3*01/02/03 - One Lambda MicroSSP ABDR

24D38-2 DRB1*08:02 実施なし実施なし実施なし DQB1*03:01/21/22/+ 実施なし Invitrogen

Allset Gold SSP HLA-DRB1*08/12 Allset Gold SSP HLA-DQB1*03

DPB1 SSP Unitray

24D39-2 DRB1*08:02/09 DRB1*14：02/06/29 DRB3*01:01/02：02 ― DQB1*04:02 DQB1*03:01 ベリタス Micro SSP

24D41-2 DRB1*08:01/03/04/+ DRB1*14:01/02/05/+ DRB3*01/02/03 - DQB1*03:01/04/09/+ DQB1*04:01/02/04/+ べりタス SSP JPN

24D42-2 DRB1*03:17/40/41 DRB1*08:09/21/35 DRB3*01//+ - DQB1*04:01/02/04/+ DQB1*03:01/04/09/+ ワンラムダ Micro SSP JPN

24D43-2 DRB1*08:01/02/03/+ DRB1*14:02/05/06/+ DRB3*01/02/03 - DQB1*03:01/04/09/+ DQB1*04:01/02/04/+ ONE LAMBDA SSP2L

24D49-2 DRB1*08:01/13:10 DRB1*14:01/02/05/+ DRB3*01:01/02:01/+ DQB1*03:01/04/09/+ DQB1*04:03 ONELAMBDA Micoro SSP DNA typing Trars SSP JPN

24D51-2 DRB1*08:02/04/13/+ DRB1*14:01/02/05/+ DRB3*01/02/03 - DQB1*03:01/04/09/+ DQB1*04:01/02/04/+ One Lambda MicroSSP JPN ABC/DRDQ

HLA-A HLA-B HLA-C HLA-DRB1 HLA-

DRB3/4/5 HLA-DQB1

H2401

A*02:03 A*24:02 B*52:01 B*56:01 C*01:01 C*12:02 DRB1* 13:12

DRB1* 15:02

DRB3* 02:02

DRB5* 01:02

DQB1* 03:01

DQB1* 06:01

A203 A24 B52 B56 Cw1 Cw12 DR13 DR15 DR52 DR51 DQ7 DQ6

H2402

A*11:01 A*31:01 B*46:01 - C*01:02 - DRB1* 09:01

DRB1* 12:01

DRB3* 01:01

DRB4* 01:01

DQB1* 03:01

DQB1* 03:03

A11 A31 B46 - Cw1 - DR9 DR12 DR52 DR53 DQ7 DQ9

H2403

A*02:06 A*11:02 B*38:02 B*48:01 C*07:02 C*08:03 DRB1* 04:10

DRB1* 16:02

DRB4* 01:01

DRB5* 02:02

DQB1* 04:02

DQB1* 05:02

A2 A11 B38 B48 Cw7 Cw8 DR4 DR16 DR53 DR51 DQ4 DQ5

H2404

A*02:01 A*24:02 B*15:05 B*51:01 C*07:02 C*14:02 DRB1* 08:02

DRB1* 14:06

DRB3* 02:02

- DQB1* 03:01

DQB1* 04:02

A2 A24 B62 B51 Cw7 Cw14 DR8 DR14 DR52 - DQ7 DQ4

第2区域表記

HLA-A HLA-B HLA-C

DNA型 A*02:03 A*24:02 B*52:01 B*56:01 C*01:01 C*12:02

HLA型 A203 A24 B52 B56 Cw1 Cw12

正解数報告数

23 23

23 23

24 25

24 25

20 20

18 20

HLA-DRB1 HLA-DRB3/4/5 HLA-DQB1

DRB1*13:12 DRB1*15:02 DRB3*02:02 DRB5*01:02 DQB1*03:01 DQB1*06:01

DR13 DR15 DR52 DR51 DQ7 DQ6

23 25

24 24

19 20

19 20

19 19

19 19

B*52:01,B*56:01

⇒B*56:07,B*78:06 （3H false negative?）24D39

C*01:02,C*12:02

⇒C*01:01//+,C*01:01//+（反応問題なし）24D31

⇒C*01:02/03/05/+, - （反応問題なし）24D42

DRB1*13:12,DRB1*15:02

⇒DRB1*13:01/14:22/+,DRB1*15:01/02/03/+

（反応問題なし）24D31

⇒DRB1*13:01/02/07,DRB1*15:01/02


DRB3*02:02,DRB5*01:02

⇒DRB3*,DRB5*（表記?）24D22

HLA-A HLA-B HLA-C

DNA型 A*11:01 A*31:01 B*46:01 - C*01:02 -

HLA型 A11 A31 B46 - Cw1 -

正解数報告数

24 24

24 24

25 25

22 24

20 20

19 19




24 24

23 23

19 20

19 20

19 19

19 19

B*46:01,-

⇒B*46:01/02/04/+,undefined （反応問題なし）24D22

⇒B*15:143/27:26/+,B*46:01/02/04/+ （反応問題なし）24D31

DRB3*01:01,DRB4*01:01


HLA-A HLA-B HLA-C

DNA型 A*02:06 A*11:02 B*38:02 B*48:01 C*07:02 C*08:03


正解数報告数

24 24

24 24

23 24

24 24

19 19

18 19




22 23

23 23

18 20

19 20

18 19

19 19

B*38:02,B*48:01

⇒B*38:01/08/09/+,B*48:01/07/09/+

（7H false negative? 表記?）24D49

C*07:02,C*08:03

⇒ C*07:01//+,C*07:101//+（反応問題なし）24D31

DRB1*04:10,DRB1*16:02

⇒DRB1*04:01,DRB1*16:02/11/14/+（反応問題なし）24D18 DRB4*01:01,DRB5*02:02

⇒DRB4*01:03,DRB5*02:02（反応問題なし）24D39


DQB1*04:02,DQB1*05:02

⇒DQB1*04:03,DQB1*05:01/02/03/+

（12B false Positive?）24D49

HLA-A HLA-B HLA-C

DNA型 A*02:01 A*24:02 B*15:05 B*51:01 C*07:02 C*14:02


正解数報告数

24 24

23 24

26 26

25 25

19 20

19 20


DRB1*08:02 DRB1*14:06 DRB3*02:02 - DQB1*03:01 DQB1*04:02

DR8 DR14 DR52 - DQ7 DQ4

22 26

23 25

19 21

18 21

20 20

19 20

A*02:01,A*24:02

⇒A*02/24,undefind（表記?）24D13

C*07:02,C*14:02

⇒C*06:11//+,C*07:64//+（反応問題なし）24D31

DRB1*08:02,DRB1*14:06

⇒DRB1*08/13/14,undefind（表記?）24D13

⇒DRB1*08:01/03/04/+,DRB1*14:01/02/05/+

（10C false negative?）24D41

⇒DRB1*03:17/40/41,DRB1*08:09/21/35


⇒DRB1*08:01/13:10,DRB1*14:01/02/05/+（表記?）24D49

DRB3*02:02,-

⇒DRB3*, - （表記?）24D22

⇒DRB3*01:01//+,DRB3*01:01//+（表記?）24D31

⇒DRB3*01:01/02:01/+, （1allele未記入）24D49

DQB1*03:01,DQB1*04:02

⇒DQB1*03:01/04/09/+,DQB1*04:03

（12B false positive?）24D49

False negative False positive

H2401 24D39(3H) -

H2402 - -

H2403 24D49(7H) 24D49(12B)

H2404 24D13(9EFG) 24D41(10C) 24D27(8A)

24D49(12B)

`24D13 `24D27 miss assign なし

• 検体（ヘパリン血不可）

• DNA純度：OD値1.6-1.9 濃度50-100ng/μ l

• 滅菌蒸留水で希釈 DNA抽出

• Taq polymeraseはAmpli Taq

• D-mixの溶液はピンク～紫色（アルカリ）

• ウェルの壁面からアプライ

• PCRプログラムは9600モード、電源

PCR増幅

• 泳動ゲルは測定ごとに作成

• PCR産物は8連ピペットで素早くアプライ

• 泳動時間注意電気泳動

Documents

輸血部 藤井 明美 - UMINsquare.umin.ac.jp/.../qcws/file/qcws/16qcws/16QC_DNA_SSP.pdf輸血関連 臓器移植 造血幹細 胞移植 SSP単独 SSP+ SSO,SBT 2 0 9 2 0 0 3 0 0

輸血部藤井明美 - UMINsquare.umin.ac.jp/.../qcws/file/qcws/16qcws/16QC_DNA_SSP.pdf輸血関連臓器移植造血幹細胞移植 SSP単独 SSP+ SSO,SBT 2 0 9 2 0 0 3 0 0