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ÆÇÈÉÊfË12~ gh ij kl mno pqr 1. `â_S,,(``* 350002 2. `â_S,Ó±±ãä-./``* 350002 34 sõtu%Bòó¢W»nv DNAòówxvyzjòó{4,¥¦%Bòóf>h+é|{ DNA}~T¾ sõvv)?RµÚ#)APFhHµsõtuB4)s1sõtu%B)v¥¦; sõtu%\ÑÒÂMÂ;sõtu%BníÏPmsõtu%BÐh%BEd hU;%Bòóµsõtu%B)h»á⫾Bsõtu%B¥h>h567 tu%B|#b®ÓX\\ 89:;< S571.1  =>?@A A   =BC< 0517-6611 2019 12-0014-05 doi 10.3969/j.issn.0517-6611.2019.12.004      DEFGHIJK?@AOSID ): ResearchProgressonGeneticTransformationSystem of Camelliasinensis CHENLan ZHUChen LIXiaozhen etal 1.CollegeofHorticulture FujianAgricultureandForestryUniversity Fuzhou Fujian 350002 2.InstituteofHorticulturalBiotechnology FujianAgricultureandForestryUniversity Fuzhou Fujian350002 Abstract Genetictransformationtechnologyof Camelliasinensis referstotheprocessofusingrecombinantDNAtechnology celltissueculture technologyorgermplasmsystemtransformationtechnologytopurposelyinsertaforeigngeneorDNAfragmentintotherecipientgenomeof Ca melliasinensis andexpressitintheprogenyplants.Itplayedanimportantroleingeneticimprovementandvarietyselectionof Camelliasinensis Wereviewedthesystemofreceptorregenerationandgenetictransformationof Camelliasinensisinvitro andrecentadvanceingenetictransfor mationof Camelliasinensis werediscussed.Andalsorevealedtheproblemsoflowtransformationefficiencyanddifficulty invitro regenerationof thecurrentgenetictransformationresearchinstituteof Camelliasinensis .Consequently plantaagrobacteriummediatedwasproposedtoachieve thepurposeofoptimizingthegenetictransformationsystemof Camelliasinensis Keywords Genetictransformation Explant Agrobacteriummediated Bombardmentmediated +LMN ;ð CLo>2018J01701 ); ;bc_C ò¡¢£Lo>CXZX2017164 CXZX2018069 ); ; bc_C¤¥¦§secòó¨©>11899170102 ); ;lC;<ª«>102/71201801101 ); sõ4, dB`Lad$CK1518023A )。 OPQR gh1995 —), û;¬-w!"dÏs õd$òóad$BC°±ab/"sõd$òóad$BCSTUV 2018-12-09 WXUV 2018-12-20 Ó±×/ÚÍÏ{RMÓ DNAxy« ÓÔþæ xyDµ%èéÚÍxyïÊ>$>CàÜFD DNA7 ãB«t}tÓ±ÜFÓÐ~ù£À\HÓØйus >çäÓ±×/ÚÍtèíî G;KÓ±×/ÚÍ> }ttèÓ±×/ÚÍ>;E¹FÚÜFÓØê h» 20 º» 70 eHCÓ±×/ÚÍxy©GÿÛS ÜFw23>ÖqÀÓ±¹us×/ÚÍ xy5ÙE4°µ>oRgÚ±OËK-µ>Ü Fãä#$?"K>jk~q#xâ½ß#$K >ê Û7Camelliasinensis L. O.Kuntze 7e)´Ü Ý>ÞAÓ±ÊPÛ7°µ>»6;EoÏE4èé°µ «¥°µ ðÛ7>°Wn/¥©ï¹Fã ÜÝSUò°µãO¶ÙSRs g- ÑM6>â½±Û7ÜFÚ}tMS 4-5 ×/ÚÍxyß]ÀGÚ-µ>j°WnÛ7> Å°µè>vqÛ7àUõ>[j æç×/ÚÍ xy>ÊËÜFÅ«Û7Ðþ°ØxG%àU4 >Û7-µùÛ7-µ'Ãj>¦7;Åþ°» ø Ûú>ÿÛ 1 ÆÇÌËÍlÎÏ >}tèéÏÛ7×/ÚÍ>M6ÜÝÛ 7}tèé>ZíîCÓt>jkFt>íþæ Ü>jkÓ±ËòCàá±>jktâÍ& ríFò1.1 ÐÑËlÒÓ jkç¢>}tèéZ> ·¿Û7tE->þæ;Ùï£þæãäÓÔþ æ+CÛ7-µt>õuqt>jk9ï ?>Û7>åãÀ£þæÐêG4Û7£ /£Û74ÄÅtæMondal í ¹LúÄÅ >tægÚÍ}tÛ7>ÚÜFÓØSaini í Lv í 10 D&LúÄÅtæ÷Û7×/ÚÍ9 ÚÜFÓØ8Û7÷×/ÚÍ© xð9'À§Nuvg6@GÚ>Û7-µ©¸µ LDLúÏ«^t×/BòtO'À©S)L ú>tþæ×/Úͦ6©jOMondal í Z> ÚÍtèIJ¦6 490d g!3G%U>×/Sandal í 11 :gÛ7×/ÚÍ>}Û7ú7G4L úðÛ7ú7>tüÆ 0~7.1% 12 øÛ7åãÀ Û7×/ÚÍIïÙS>þÀÛ7åãþæÐ6jk ï?ö}Éóåæ@>åãï-.ud_;6ç è>åãgtÆèüQx 13 Û7ãäÓÔ9Ï×/ÚÍÐR©>}t]&© ÌCÓt/¦ÄÅãäÓÔ>jO©Ì´ÄÅãäÓÔ ghijFGJ.AnhuiAgric.Sci.2019 47 12 ): 14-18 23

É˚f¸†12~ · 2019-06-18 · >jOg 7~l0d,Lœ‹2ƒ6 8~12d,媃6 9~12d, łœ7ƒ6 20d[14]。flCÓt>8⁄A_@˝ıł¨8» ˙7>ªäÓÔ¿,š›÷”âØvî¯>Û7×/Ú˝,

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Page 1: É˚f¸†12~ · 2019-06-18 · >jOg 7~l0d,Lœ‹2ƒ6 8~12d,媃6 9~12d, łœ7ƒ6 20d[14]。flCÓt>8⁄A_@˝ıł¨8» ˙7>ªäÓÔ¿,š›÷”âØvî¯>Û7×/Ú˝,

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ResearchProgressonGeneticTransformationSystemofCamelliasinensisCHENLan1,ZHUChen1,2,LIXiaozhen1etal (1.CollegeofHorticulture,FujianAgricultureandForestryUniversity,Fuzhou,Fujian,350002;2.InstituteofHorticulturalBiotechnology,FujianAgricultureandForestryUniversity,Fuzhou,Fujian350002)Abstract GenetictransformationtechnologyofCamelliasinensisreferstotheprocessofusingrecombinantDNAtechnology,celltissueculturetechnologyorgermplasmsystemtransformationtechnologytopurposelyinsertaforeigngeneorDNAfragmentintotherecipientgenomeofCamelliasinensisandexpressitintheprogenyplants.ItplayedanimportantroleingeneticimprovementandvarietyselectionofCamelliasinensis.WereviewedthesystemofreceptorregenerationandgenetictransformationofCamelliasinensisinvitro,andrecentadvanceingenetictransformationofCamelliasinensiswerediscussed.AndalsorevealedtheproblemsoflowtransformationefficiencyanddifficultyinvitroregenerationofthecurrentgenetictransformationresearchinstituteofCamelliasinensis.Consequently,plantaagrobacteriummediatedwasproposedtoachievethepurposeofoptimizingthegenetictransformationsystemofCamelliasinensis.Keywords Genetictransformation;Explant;Agrobacteriummediated;Bombardmentmediated

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