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KI M NGHIM VIN NNBERBERIN CLORID

NH TNH

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NH LNG B NG PHNG PH PO QUANG PH HP TH UV-VIS

THEO DVN III Dung dchth: Cn 0,2g chphmv ha tantrong 200ml ncbngcch unnng. Sau lmlnhv thm ncn1000ml. Ly10 ml dung

dchny pha long vincn100ml (C=20g/ml) Dung dchchuncpha tngtdung dch

th, dng chtichiuberberin clorid ohpthcadung dchthv dung dch

chuntrong cng iukintibcsng cci421 nm vimutrngl ncct, ccody 1cm

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Cch tnh ktqu:Theo phngphp so snh, hm lngphntrmctnh theo cng thc:

Trong

C%: Hm lng% berberin clorid c trong muthC : Hm lng% berberin clorid c trong muchunAt: hpthcadung dchth

Ac: hpthcadung dchchunmt: KL (g) caberberin clorid trong btnguyn liucn pha dung dchthmc: KL (g) caberberin clorid trong btnguyn liucn pha dung dchchun

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KIM NGHIM THUC TIMVITAMIN B12

NH TNH

Phhpthcadung dchmuthghi cphn nhlng phic 3 ccihpth278 + 1nm , 361 + 1nm, 550 + 2nm

Tshpth: A278/ A361= 0.57 + 0.02

A550/ A361= 0.3 + 0.02

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NH LNG Nguyn tc:Do c hniitrong phn t, Vitamin B12 hpthbcxUV-VIS

Tinhnh:Pha long dung dchchphmvinccnng25 mcg/ml

ohpth bcsng 361 nm, muichngl nc

Tnh hm lngvitamin B12 theo E1%1cm=207

max= 361 nm