National Cheng Kung University

Ph.D. in Engineering Science 2006~2011 Dissertation: Integrated Microfluidic System for Rapid Screening of Aptamers

Advisor: Prof. Gwo-Bin Lee

M.S. in Nanotechnology and Microsystems Engineering 2004~2006

Thesis: Integrated Microfluidic System for Glucose Sensing and Automatic Insulin Injection

Advisor: Prof. Gwo-Bin Lee

HONOR 中國機械工程學會博士論文獎 佳作 (2011)

九十五年度全國力學年會學生論文競賽固力材料組 第二名 (2006)

PATENT 1. “篩選適合體之微流體晶片系統及其使用方法,” 發明人：李國賓、謝淑珠、黃朝均、

林欣億；中華民國發明專利，專利編號：201040526

2. “適合體及 C-反應蛋白檢測方法,” 發明人：李國賓、謝淑珠、黃朝均、林欣億；中華

民國發明專利，專利編號：201200874

3. “ Aptamer and detection method for c-reactive protein,” Inventors: G. B. Lee, S. C. Shiesh,

C. J. Huang, H. I. Lin, patent filed to USPTO on 2010/9/22 with a filing number 12/924,225.

*李國賓老師(G. B. Lee)和謝淑珠老師(S. C. Shiesh)為本人博士班之指導教授人

PUBLICATION

Journal Paper 1. C. J. Huang, Y. H. Chen, C. H. Wang, T. C. Chou and G. B. Lee, “Integrated Microfluidic

Systems for Automatic Glucose Sensing and Insulin Injection,” Sensors and Actuators B:

Chemical, vol. 122, pp. 421-428, 2007. (SCI; Impact factor=2.646)

2. C. J. Huang, C. C. Lu, T. Y. Lin, T. C. Chou and G. B. Lee, “Electrochemical Albumin Sensing

System Utilizing Microfluidic Technology,” Journal of Micromechanics and

Microengineering, vol. 17, pp. 835-842, 2007. (SCI; Impact factor=2.499)

3. C. J. Huang, H. I. Lin, S. C. Shiesh and G. B. Lee, “Integrated Microfluidic System for Rapid

Screening of CRP Aptamers Utilizing Systematic Evolution of Ligands by Exponential

Enrichment (SELEX),” Biosensors and Bioelectronics, vol. 25, pp. 1761-1766, 2010. (SCI;

Impact factor=5.43) 2/70=2.9% (CHEMISTRY, ANALYTICAL)

1. C. C. Lin, C. C. Tseng, C. J. Huang, J. H. Wang and G. B. Lee, “An Integrated Microfluidic Chip

for Non-immunological Determination of Urinary Albumin,” Biomedical Microdevices, vol.

12, pp. 887-896, 2010. (SCI; Impact factor=3.323) 7/59=11.9% (ENGINEERING,

BIOMEDICAL)

2. C. J. Huang, J. L. Lin, P. H. Chen, M. J. Syu and G. B. Lee, “A Multi-functional Electrochemical

Sensing System Using Microfluidic Technology for the Detection of Urea and Creatinine,”

Electrophoresis, vol. 32, pp. 931-938, 2011.

3. C. J. Huang, H. C. Chien, T. C. Chou and G. B. Lee, “Integrated Microfluidic System for

Electrochemical Sensing of Glycoslated Hemoglobin,” Microfluidics and Nanofluidics, vol.

10, pp. 37-45, 2011. (SCI; Impact factor=3.286) 3/56=5.4% (INSTRUMENTS &

INSTRUMENTATION )

4. Y. H. Chang, C. J. Huang and G. B. Lee, “A tunable microfluidic-based filter modulated by

pneumatic pressure for blood cell separation,” Microfluidics and Nanofluidics, vol. 12, pp.

85-94, 2011. (SCI; Impact factor=3.286) 3/56=5.4% (INSTRUMENTS & INSTRUMENTATION)

5. Y. H. Chen, H. I. Lin, C. J. Huang, S. C. Shiesh and G. B. Lee, “An Automatic Microfluidic

System that Continuously Performs the Systematic Evolution of Ligands by Exponential

Enrichment,” Microfluidics and Nanofluidics, Microfluidics and Nanofluidics, vol. 12, pp.

85-94, 2012. (SCI; Impact factor=3.286) 3/56=5.4% (INSTRUMENTS & INSTRUMENTATION)

6. C. H. Weng, C. J. Huang and G. B. Lee, “Screening of aptamers on microfluidic systems for

clinical applications,” Sensors, vol. 12, pp. 9514-9529, 2012. (SCI; Impact factor=1.739)

7. C. J. Huang, H. I. Lin, S. C. Shiesh and G. B. Lee, “An integrated microfluidic system for rapid

screening of alpha-fetoprotein-specific aptamers,” Biosensors and Bioelectronics, vol. 35,

pp.50-55, 2012. (SCI; IF=5.44) 1/26=3.8% (ELECTROCHEMISTRY) 8. 黃朝均、李國賓, “整合微流體之電化學感測晶片於生醫檢測之應用,” 化工技術月刊,

2012年, 第231期, 6月號

Conference Paper 1. C. J. Huang, C. C. Lu, G. B. Lee, Thong-Yueh Lin, "Automatic Microfluidic Chips for

Electrochemical Detection of Albumin", The 2nd International Meeting on Microsensors

and Microsystems. (15-18 January 2006 @ National Cheng Kung University, Tainan,

Taiwan, R.O.C.) (oral presentation)

2. Y. H. Chen, C. J. Huang, G. B. Lee, T. C. Chou, "Microfluidic Glucose Planar Sensor Generated

by Galvanostatic Immobilization GOD with Polypyrrole", The 2nd International Meeting on

Microsensors and Microsystems. (15-18 January 2006 @ National Cheng Kung University,

Tainan, Taiwan, R.O.C.)

3. C. H. Chang, C. Y. Huang, X. F. Li, C. C. Lu, T. Y. Lin, G. B. Lee, C. J. Huang and T. C. Chou,

"Design of a Scan Mode Potentiostat for Micro-Albumin Sensor", The 2nd International

Meeting on Microsensors and Microsystems. (15-18 January 2006 @ National Cheng Kung

University, Tainan, Taiwan, R.O.C.)

4. C. J Huang, Y. H. Chen, T. C. Chou and G. B. Lee, "Microfluidic systems for glucose sensing

and insulin injection," submitted to The 9th Nano Engineering and Micro System

Technology Conference (Nov., 2005, Southern Taiwan University of Technology, Tainan,

Taiwan) (oral presentation)

5. 陳逸炘, 李國賓, 黃朝均, 周澤川,"微流體聚仳咯固定化葡萄糖氧化酵素平面式感

測器",第十一屆化學感測科技研討會（嘉義,吳鳳技術學院,2005,05）。

6. C. J. Huang, Y. H. Chen, T. C. Chou and G. B. Lee, “Microfluidic Systems for Glucose Sensing

and Insulin Injection,” The 30th National Conference on Theoretical and Applied

Mechanics, December 15-16, 2006. (oral presentation)

7. C. J. Huang, H. I. Lin, S. C. Shiesh and G. B. Lee, “Integrated Microfluidic System for Rapid

Selection of CRP Aptamers Utilizing Systematic Evolution of Ligands by Exponentail

Enrichment,” Micro-TAS 2009.

8. C. J. Huang, H. C. Chien, T. C. Chou and G. B. Lee, “Integrated Microfluidic System for

Electrochemical Sensing of Glycoslated Hemoglobin,” IEEE NANOMED, 2009. (oral

presentation)

9. C. J. Huang, H. I. Lin, S. C. Shiesh and G. B. Lee, “Systematic Evolution of Ligands Exponential

Enrichment Using Microfluidic technology for Selection of C-Reactive Protein Aptamers,”

The 13rd Nano Engineering and Micro System Technology Conference, 2009. (oral

presentation)

10. W. B. Lee, Y. H. Chen, H. I. Lin, C. J. Huang, S. C. Shiesh and G. B. Lee, “A Microfluidic System

Integrated with Optical Detection Devices for Automatic Detection of C-Reactive Protein,”

The 23th IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS

10), Hong Kong, China, Jan. 24-28, 2010.

11. C. J. Huang, J. L. Lin, M. J. Syu and G. B. Lee, “ Multi-functional Electrochemical Detection

System Using Microfluidic Technology,” The 5 Asia-Pacific Conference on Transducers and

Micro-Nano Technology, Perth, Western Australia, July 6-9, 2010. (oral presentation)

12. C. C. Lin, C. C. Tseng, C. J. Huang, J. H. Wang and G. B. Lee, “Non-immunological

Determination of Microalbuminuria by Using a Microfluidic chip,” The 5 Asia-Pacific

Conference on Transducers and Micro-Nano Technology (APCOT), Perth, Western

Australia, July 6-9, 2010.

13. Y. H. Chang, C. J. Huang and G. B. Lee “A tunable microfluidic-based filter modulated by

pneumatic pressure for blood cell separation,” The 34th National Conference on

Theoretical and Applied Mechanics, November 19-20, 2010.

14. C. J. Huang, H. I. Lin, S. C. Shiesh and G. B. Lee, “An integrated Microfluidic System for rapid

screening of alfa-fetoprotein aptamers,” The 24th International Conference on Micro

Electro Mechanical Systems (IEEE MEMS 11), Cancun, Mexico, January 23-27, 2011. (oral

presentation)

15. C. J. Huang, H. I. Lin, S. C. Shiesh and G. B. Lee, “Rapid Measurement of AFP Using AFP-

Specific Aptamer on a Microfluidic Chip,” The 25th International Conference on Micro

Electro Mechanical Systems (IEEE MEMS 12), Paris, France, 29 January - 2 February, 2012.

中國機械工程學會博士論文獎佳作

九十五年度全國力學年會學生論文競賽固力材料組第二名