ORGANISING COMMITTEE 2012.pdf · OWLS • Genoa, 4-6 July 2012 • Program • Page 5 OWLS 2012 Tuesday, July 3rd - 7.00pm Inaugural Ceremony Sala del Maggior Consiglio, Palazzo Ducale

OWLS 2012

ORGANISING COMMITTEEAlberto Diaspro, Istituto Italiano di Tecnologia and University of Genoa, Italy Francesco Baldini, National Research Council, Florence, ItalyPaolo Bianchini, Istituto Italiano di Tecnologia, Genoa, ItalyFrancesca Cella Zanacchi, Istituto Italiano di Tecnologia, Genova, ItalyValeria Delle Cave,Istituto Italiano di Tecnologia, Genova, ItalyZeno Lavagnino, Istituto Italiano di Tecnologia and University of Genoa, Italy Mattia Pesce, Istituto Italiano di Tecnologia, Genova, ItalyManuela Rigano, University of Genova, ItalyManuela Salvatori, Istituto Italiano di Tecnologia, Genova, ItalyFederico Tixi, Istituto Italiano di Tecnologia, Genova, ItalyCesare Usai, National Research Council, Genova, Italy

PROGRAMAlberto Diaspro, Istituto Italiano di Tecnologia and University of Genoa, ItalySilvio Aime, University of Turin, ItalyFrancesco Baldini, National Research Council, Florence, ItalyGert von Bally, University of Muenster, GermanyPaolo Bianchini, Istituto Italiano di Tecnologia, Genoa, ItalyFrancesca Cagnoni, Istituto Italiano di Tecnologia, Genova, ItalyHalina Rubenstein-Dunlop, University of Queensland, AustraliaHans-Jochen Foth, Technical University of Kaiserslauter, GermanyMin Gu, Swinburne University, AustraliaPartha Pratim Mondal, Indian Institute of Science, Bangalore, IndiaStephen Morgan, University of Nottingham, UKColin JR Sheppard, National University of Singapore, SingaporeCesare Usai, National Research Council, Genova, Italy

OWLS • Genoa, 4-6 July 2012 • Program • Page 1

Page 2 • Program • OWLS • Genoa, 4-6 July 2012

Exhibit Area

2nd FloorG Nikon Instruments SpaH IIT - Istituto Italiano di Tecnologia / LANIR.EUI Physik Instrumente (PI) S.r.l.L HAMAMATSU PHOTONICS ITALIAM Till Photonics GmbHN Fianium Limited


3rd FloorA OLYMPUS ITALIA SPAB VUTARA Inc.C TOPTICA Photonics AGD Bitplane AGE-F Leica Microsystems Srl



OWLS 2012

Tuesday, July 3rd - 7.00pm

Inaugural Ceremony

Sala del Maggior Consiglio, Palazzo Ducalewww.palazzoducale.genova.it

Alberto Diaspro, OWLS President

Roberto Cingolani, Scientific Director, Istituto Italiano di Tecnologia

“Lettura Magistrale”Scott E. Fraser, Caltech, CA

Imaging the Molecular and Cellular Dynamics of Embryogenesis

Welcome Cocktail kindly offered by Camera di Commercio di Genova

Leica MicrosystemsBitplane


Wednesday, July 4th

9.00 - 9.30 Magazzini del Cotone - Congress Center http://www.portoantico.it

Scirocco-Libeccio RoomOWLS 2012 Opening LectureKarsten Konig, Saarbrucken University, GermanyClinical Multimodal Hybrid Multiphoton/CARS Tomography of In Vivo Human Skin

Chair: A. Diaspro

Parallel Sessions9.30 - 10.00 Scirocco - Libeccio Room Ponente Room

David SampsonUniversity of Western Australia, AustraliaOptical elastography - high-resolution imaging of tissue mechanical properties.

Chair: G. Scarcelli

Gertrude BuntUniversity of Goettingen, GermanyVisualization of multiple coupled signal-ing events in a single cell by fluorescence lifetime pattern analysis.

Chair: R. Bizzarri10.00 - 10.45 Optical application to biomedicine

Chair: G.Scarcelli

Andrea CuratoloUniversity of Western Australia, Australia Portable 3D optical coherence elastogra-phy for applying microscopic imaging of tissue mechanical properties in the clinic.

George A. StanciuUniversity Politehnica Bucharest, RomaniaElectrical charge on the hydroxyapatite surface investigated by laser scanning microscopy.

S. BekeIstituto Italiano di Tecnologia (IIT), Gen-ova, ItalyExcimer laser-based stereolithography for rapid fabrication of rigid biodegrad-able photopolymer scaffolds for tissue engineering.

Advanced imaging techniques I Chair: R. Bizzarri

Barbara StortiNEST, Pisa Italy Multiplexing correlation spectroscopy and resonance energy transfer unveils dy-namical interactions between transient receptor potential vanilloid 1 (trpv1) and microtubules at nanoscale in living cells.

Dominika O. Trembecka-LucasJagiellonian Universit. PolandInteraction between HP1 dimer and PCNA in DNA replication and repair, as revealed by bimolecular fluorescence complementation assay (BiFC), and FRET/FLIM.

Simona RodighieroFondazione Filarete, Milan, ItalyFRET study of 4.1R, ICln and actin inter-actions: a link between cell morphology and cell proliferation.


10.45 - 11.15 Giuliano ScarcelliHarvard Medical School, USABrillouin microscopy to study the bio-mechanical properties of cornea and lens.

Chair: D.Sampson

Ranieri BizzarriNational Research Council, ItalyNovel probes and strategies for high-res-olution fluorescence imaging in living cells.

Chair: G.Bunt

11.15 - 11.45 Coffee break11.45 - 12.15 Scirocco-Libeccio Room

Monika Ritsch-MarteMedizinische Universität Innsbruck, AustriaApplications of spatial light modulators in biophotonics: from optical tweezers to advanced microscopy.

Chair: F.Difato


Plasmonics Chair: E. Di Fabrizio

Emanuela EspositoCNR-ICIB Napoli, ItalyLab-on-Fiber Technology: Nanophoton-ics Within Optical Fibers.

Fang LiuTsinghua University, BeijingIntegrated Bio-Sensor based on Hybrid Coupler with Surface Plasmon Polariton and Dielectric Waveguide.

Ranjeet KumarIndian Institute of Technology New Delhi, IndiaClustering of Au-NPs near plasmon ex-citation and enhanced scattering based in-vitro detection of Ehrlich ascites car-cinoma cells.

Advanced imaging techniques II Chair: A. Periasamy

Antonella BattistiNEST, Pisa, ItalyIntracellular pH measurements made simple by genetically-encoded fluo-rescent probes and phasor approach to FLIM.

Ottavia GolfettoUniversity of California, Irvine, CaliforniaLaurdan fluorescence lifetime as a bi-osensor for membrane fluidity.

Deo R. SinghUniversity of Wisconsin Milwaukee, USAQuaternary structure of wild type and mutated rhodopsin at room and body temperature using spectral FRET.

13.30 - 15.30 Lunch, posters, exhibition, Job corner.

Company Presentations14.00 - 15.00 Scirocco - Libeccio Room Ponente Room

Olga LevaiLeica MicrosystemsOpening the gate to Super-Resolution.

Maria MarosvölgyiTill PhotonicsCombining advanced fluorescence imag-ing techniques on the same living sample: spinning disk confocal, TIRF, FRAP(PA) and FRET.


15.30 - 16.00 Scirocco - Libeccio RoomAnthony Watts Oxford University, UKRealizing bio-imaging potential: Enhancing bacteriorhodopsin-mediated

molecular scale, wavelength-selective photoswitching.

Chair: A. Periasamy


Ilaria Testa Max Planck Institute Goettingen, GermanyRESOLFT Nanoscopy in Life Sciences: unraveling the mysteries with low light levels

Chair: G. Vicidomini

Arthur ChiouNational Yang-Ming University, Taiwan (R.O.C.)Optical Tweezers Based BioMicrorheology

Chair: D. Cojoc

16.30 - 17.15 Single molecule and super-resolution Chair: I.Testa

Paolo AnnibaleEPFL, Lausanne, SwitzerlandTowards accurate dual color PALM im-aging.

Francesca Cella ZanacchiIstituto Italiano di Tecnologia, Genova, ItalySuper-resolution microscopy of large biological samples within a light sheet based architecture.

Davide MazzaFondazione S.Raffaele, Milano, ItalyMeasurement of binding in living cells by single molecule tracking. A benchmark for protein/scaffold interactions.

Optical Tweezers Chair: A. Chiou

Francesco DifatoIstituto Italiano di Tecnologia, Genova, ItalyPosition and Force-clamp interferometric tracking of an optically trapped probe to study cytoskeleton dynamics of devel-oping mouse neuron.

Pasquale BiancoUniversità degli Studi di Firenze, ItalyThe energy landscape of DNA over-stretching transition determined with a Dual Laser Optical Tweezers under force clamp.

Dan CojocMaterials Technology Institute, Trieste, ItalyLocal delivery of small numbers of attrac-tant and repulsive guidance molecules to neuronal cells by optical manipulation techniques.

17.15 - 17.45 Mike HeilemannWuerzburg University, GermanyQuantitative Single Molecule Biology with Photoswitchable Fluorophores.

Chair: I.Testa

Maria Luisa Calvo PadillaUniversidad Complutense de Madrid, SpainHolography and its applications.

Chair: A.Chiou


17:45 - 18:15 Coffee break18:15 - 18:45 Scirocco - Libeccio Room

Brad Amos Cambridge, UKA laser-scanning microscope for optical sectioning of large-volume specimens such as mouse embryos using a Mesolens.

Chair: C.J.R. Sheppard


Optical tomographyChair: C.J.R. Sheppard

Stephen P MorganUniversity Park, Nottingham, UKPulsed ultrasound modulated optical to-mography of tissue

Maria PogorelovaMoscow State University, Moscow, Russian FederationOptical microtomography of early mouse embryo with laser scanning microscopy

Ludovico SilvestriUniversity of Florence, ItalyWhole-brain neuroanatomy on the mi-cron-scale with confocal light sheet mi-croscopy

Optical application to biomedicine IIChair: K. Braeckmans

Wei ZhangTsinghua University, Beijing, ChinaTrace level multi-gas sensing in mid-in-frared hollow-core Bragg fiber supporting multi-wavelength transmission

TadelechLaser-Induced Breakdown and X-Ray Fluorescence Spectroscopic Techniques in Detection of Elements in Tea Leaves

Tewfik SoulimaneUniversity of Limerick, IrelandPhotosterilisable Textiles for Improved Hospital Hygiene

19.30-20.00 Martin J. Booth Oxford University, UKAdaptive optics for high resolution bio-logical microscopy

Chair: C.J.R. Sheppard

Dayong JinMacquarie University, Sydney, NSW 2109, AustraliaNovel coding and decoding in suspension arrays for accelerated biomolecular dis-covery and personalized medicine.

Shan Shan KouEPFL, Lausanne, CH-1015 SwitzerlandThree-dimensional (3D) Monte Carlo Simulation of Born’s Diffractions for Cel-lular Analysis


Thursday, July 5th

8.30 - 9.00 Scirocco - Libeccio Room RMS LectureTony Wilson Oxford University, UKMaking Light Work in Microscopy.

Chair: F. Brakenhoff


Martin OheimUniversité Paris Descartes, FranceNon-evanescent excitation light in ob-jective-type TIRF microscopy limits to quantitative imaging and super-resolu-tion microscopy.

Chair: J. Bewersdorf

Amitabha ChattopadhyayCentre for Cellular and Molecular Biology Hyderabad, IndiaOrganization and Dynamics of the Se-rotonin-1A Receptor in Live Cells using Fluorescence Microscopy.

Chair: S. Maiti9.30 - 10.15 STED microscopy I

Chair: J. Bewersdorf

Giuseppe VicidominiIstituto Italiano di Tecnologia, Genoa, ItalyTowards Low-Intensity STED Microscopy by Time Gating.

Lorena BenedettiUniversity of Milan, Italy Morphological features of 4.1R overex-pressing cells characterized with Correla-tive Light-Scanning Electron Microscopy and observed with gated-STED nanoscopy.

Tilman RosalesNational Institutes of Health, USA Instrumentation for super-resolution us-ing new low power probes.

Advanced Imaging Techniques III Chair: A. Chattopadhyay

Cosimo ArnesanoUniversity of California Irvine, Irvine, Cal-iforniaDigital Parallel Frequency-Domain Spec-troscopy for Tissue Imaging.

Stefano CoppolaSapienza - University of Rome, ItalyIntracellular trafficking of multicompo-nent lipoplexes: a fluorescence micros-copy study.

Anna Letizia Allegra MascaroUniversity of Florence, ItalyIn vivo imaging of climbing fibers reactive plasticity after laser axotomy.

10.15 - 10.45 Joerg BewersdorfYale University, USANew Approaches in STED Microscopy: TIRF and Phase Retrievaly.

Chair: M. Oheim

Sudipta MaitiTata Institute of Fundamental Research, IndiaProtein misfolding versus aggregation: following the early events with an ultra-sensitive fluorescence tool.

Chair: A. Chattopadhyay



10:45 - 11:15 Coffee break11.15 - 11.45 Scirocco - Libeccio Room

Ernst H.K. StelzerGoethe University Frankfurt , GermanyFrom the ultramikroskop to theta microscopy to tetrahedral microscopy to light sheet-based fluorescence microscopy.

Chair: F. Cella Zanacchi


Single Particle Tracking Chair: Z. Lavagnino

Hendrik DeschoutGhent University, BelgiumOn-chip sheet illumination for sin-gle-particle tracking measurements in bio-fluids.

Katrien ForierGhent University, BelgiumSingle particle tracking microscopy: To-wards nanomedicines for the treatment of chronic pulmonary infections in cystic fibrosis patients.

Christelle RosazzaUniversity of Konstanz, GermanySingle Particle Tracking of DNA in cells delivered by electroporation.

Label Free Techniques Chair: P. O’Shea

Francesca Romana BertaniNational Research Council, Rome, ItalyHyperspectroscopic confocal reflectance imaging of cultured cells.

Alessandro CandianiUniversity of Parma, ItalyLabel-free DNA sensor based on optical fiber grating devices.

Dan CojocNational Research Council, Trieste, ItalySecondary speckle sensing microscopy for fast detection of malaria.

12.30 - 13.00 Kevin BraeckmansUniversity of Ghent, BelgiumSizing Nanomatter in Biological Fluids by Fluorescence Single Particle Tracking.

Chair: Z. Lavagnino

Paul O’ Shea University of Nottingham, UK Imaging specific proteins in cells; time-resolved dynamics and disposition without GFP.

Chair: A. Chattopadhyay

13.00 - 15.00 Lunch, posters, exhibition, Job corner.

Company Presentations14.00-15.00 Scirocco-Libeccio Room

Luciano LucasBitplaneImaris: the 3D/4D image analysis soft-ware

Ponente RoomAlessandro ComettaZeissA super-resolution close-up view: from SIM to PALM



15.00 - 15.30 Scirocco-Libeccio RoomColin SheppardNational University of SIngapore, Singapore and Istituto Italiano di Tecnologia, ItalyImaging in the phase contrast microscope.

Chair: P.Saggau15.30 - 16.00 Gail McConnell

University of Strathclyde, UKPromising new wavelengths for multiphoton laser scanning microscopy: thinking outside the Ti:Sapphire

Chair: P.Saggau


Non Linear/Multiphoton MicroscopyChair: G. McConnell

Giulia BorileUniversity of Padova, ItalyMultiphoton fluorescence Ca2+ imaging in acute cardiac slices.

Albrecht HaaseUniversity of Trento,ItalyIn-vivo morpho-functional imaging of the honeybee brain .

B. LeshemParis Descartes UniversityTemporally focused illumination is inher-ently robust against scattering.

Leonardo SacconiNational Research Council, Florence, ItalyOptical recording of intracellular action potential propagation in cardiac myo-cytes.

Godofredo BautistaTampere University of Technology, Fin-landWide field third-harmonic generation microscopy of turbid media.

Neeven A. HosnyImperial College London, Great BritainViscosity imaging of lipid-based micro-bubbles using fluorescence lifetime im-aging (FLIM) of molecular rotors.

Fluorescent Probes/Sensors Chair: F. Brandi

Komal BaggaIstituto Italiano di Tecnologia, Genoa, ItalyLaser-based generation of luminescent bio-nanomarkers for cell imaging.

Ilaria De SantoIstituto Italiano di Tecnologia, Naples, ItalyUnderstanding Drug Localization in Dox-orubicin Loaded TiO2 Nanotube Arrays through Fluorescence Correlation Spec-troscopy analysis.

Joanna L RichensUniversity of Nottingham, UKPlasmonic-Based Biosensors in Disease Diagnosis.

Shu-Han WuNational Yang-Ming University, Taipei, TaiwanDynamic Study of Cell Secretion using Microfluidic Traps and Gold Nanoslits Based Surface Plasmon Sensors.

Milena StephanGeorg-August University of Goettingen, GermanyCombining Fluorescence Microscopy with Reflectometry: Development of a Biomembrane Sensor.



17.30 - 18.00 Scirocco - Libeccio Room Francesco S. PavoneEuropean Laboratory for Non Linear Spectroscopy, University of FlorenceIn Vivo Reactive Neural Plasticity Investigation by means of Correlative Light-Elec-tron Microscopy.

Chair: P. Bianchini18.00 - 19.15

20.30

OWLS Assembly with Coffee break

Social DinnerVilla dello Zerbinohttp://www.villalozerbino.it/


Friday, July 6th

9.00 - 9.30 Scirocco - Libeccio Room Enrico GrattonUniversity of California Irvine, USSuper-resolution by feedback imaging: mechanisms of translocation through the nuclear pore complex.

Chair: P. Bianchini9.30 - 10.00 Daniel Farkas

Cedars-Sinai Medical Center, USAMultimode Optical Molecular Imaging of Disease for Better Diagnosis and Treatment.

Chair: P. Bianchini


Amyloid Studies Chair: C. Canale

Valeria MusiEcole Polytechnique Fédérale de Lausanne, SwitzerlandBloch surface wave optical platform for sensing the aggregation kinetics of amy-loid beta peptides. Towards the early de-tection of Alzheimer’s disease.

Bidyut SarkarTata Institute of Fundamental Research, Mumbai, IndiaLabel free dopamine imaging with multi-photon ultraviolet microscopy.

Niels ZijlstraUniversity of Twente, NetherlandsEstablishing the composition of al-pha-synuclein amyloid oligomers using single-molecule photobleaching.

Optics in the environmental science Chair: F. Baldini

Malgorzata BiczyskoIstituto Italiano di Tecnologia, Pisa, ItalySimulation of optical spectra line-shapes for biomolecules in natural environmen-tal conditions.

Sanjaya ThilakarathneSaitama University, JapanUltra short term growth dynamics of Soybean and Radish under ozone stress using Statistical Interferometry Tech-nique - a comparison with conventional ozone assessment measures.

Dayong JinMacquarie University, Sydney, AustraliaTuning the Rare-earth Nanoparticles for Background-free Biosensing.

10.45 - 11.15 Coffee break11.15 - 11.45 Scirocco - Libeccio Room

Nicolai UrbanMax Planck Institute Goettingen, GermanyDynamic superresolution microscopy deep inside living brain tissue.

Chair: P. Bianchini



Sted Microscopy II Chair: B. Harke

Paolo BianchiniIstituto Italiano di Tecnologia, Genoa, Italy Single wavelength 2PE-STED nanoscopy.

Brian R. RankinMobius Photonics, CA, USAA new laser for STED

Jay R. KnutsonNational Institutes of Health, USAA new class of low-power probes for mul-ticolor nanoscopy.

Novel Techniques Chair: E. Gratton

Cristophe SilienUniversity of Limerick, EnglandChemical Imaging with Super Resolution Infra-Red Absorption.

P. NandakumarBITS Pilani K. K. Birla Goa Campus, IndiaLaser scanning photothermal microscopy.

William M. WhelanAtlantic Veterinary College, CanadaFrequency spectrum analysis in pho-toacoustic tissue characterization.

12.30 - 13.00 Peter SaggauBaylor College, Texas, USAAdvanced Optical Imaging for Re-verse-Engineering the Brain

Chair: B. Harke

Ari T. FribergNational Institute of Advanced Industrial Science and Technology (AIST), JapanClassical Ghost Imaging of Phase Objects and through Phase Aberrations

Martijn StopelUniversity of Twente, NetherlandsRoom temperature excitation spectros-copy of single quantum dots

13.00 - 15.00 Lunch, posters, exhibition, Job corner.15.00 - 15.30 Scirocco - Libeccio Room Ponente Room

Maria Garcia ParajoICFO- Institut de Ciences Fotonique Barcelona, SpainCell surface nanoscopy using single molecule optical nanotools

Chair: M. Heilemann15.30 - 16.00 EBSA Lecture

Andreas ZumbuschUniversitat Konstanz, GermanyCoherent anti-Stokes Raman Scattering microscopy of intracellular dynamics

Chair: M. Heilemann



Nanoparticles & Smart Materials

Chair: M. Lorenzoni

Athanassia AthanassiouIstituto Italiano di Tecnologia, Genoa, ItalyNanocomposite surfaces with photoin-duced wettability gradient for directional growth of cells.

Francesco BaldiniNational Research Council, Floresnce, ItalyPMMA-nanoparticles for molecular bea-con delivery in cells for survivin mRNA detection.

Despina FragouliIstituto Italiano di Tecnologia, Lecce, ItalyRendering cellulose sheets antibacterial using silver nanoparticles formed by a laser ablation technique.

Alemu KebedeAddis Ababa University, EtiopiaControlled Synthesis, Characterization and Application of Iron Oxide Nanopar-ticles for Oral Delivery of Insulin.

Enrico RampazzoUniversità di Bologna, ItalyFluorescent Silica-Core/PEG-Shell Nano-particles with Tunable emission for Imag-ing and Labeling.

Laura D’AlfonsoUniversità degli Studi di Milano Bicocca, Milan, ItalyIntracellular diffusion of Gold Branched Nanoparticles.

Force Microscopy

Chair: C. Canale

Marco CapitanioEuropean Laboratory for Non-linear Spec-troscopy, Sesto Fiorentino, ItalyUltrafast force-clamp spectroscopy of single biomolecules

Josè L.Toca-HerreraUniversity of Natural resources and Life Sciences Wien, AustriaDelivering mechanical and interfacial sample properties by combining optical and atomic force microcopies

Jenu Varghese ChackoIstituto Italiano di Tecnologia, Genoa, ItalyAFM STED: Targeted probing with high spatial precision

Raman Spectroscopy Chair: R. Proietti

Riccardo CicchiEuropean Laboratory for Non-linear Spec-troscopy, Sesto Fiorentino, ItalyA combined fluorescence-Raman spec-troscopic probe for the diagnosis of melanocytic lesions

Anna Chiara De LucaInstitute of Protein Biochemistry, Naples, ItalyModulated Raman spectroscopy: a promising biophotonics tool for early cancer diagnosis

Nicholas SmithOsaka University, JapanRaman imaging and analysis: from quan-tification of cellular morphology to RNA structure and intramolecular interac-tions.


17.30 - 18.00 Closing CeremonyScirocco-Libeccio Room

OWLS 2012 Closing LectureSatoshi KawataOsaka University, JapanPlasmons, Raman, nano and 3D, all in together.

Chair: A. Diaspro

18.00 - 18.30 Poster prizesSponsored by OWLS, Université Laval Québec, Camera di Commercio di Genova

Farewell with Focaccia and white wineSponsored by Camera di Commercio, Leica Microsystems, Bitplane

Friday, July 6 2012 8.30pmCity Lights by LAMBSwww.lambs.itPiazza De Ferrari, Genova (De Ferrari Square)

Saturday, July 7 2012 Full DayCinque Terre Tourwww.cinqueterre.it


OWLS 2012 Poster Session

“An insight of NoBoDys PerFect” Exhibition

P1 - P6 PageP1 Gaser N. Abdelrasoul1,2 , Raffaella Magrassi3, Marco Scotto d’Abbusco1, Alberto

Diaspro1, 2

1 Department of Nanophysics, Istituto Italiano di Tecnologia, via Morego 30, 16163 Genova, Italy2 Department of Physics University of Genova, via Balbi 5, 16126 Genova, Italy3 National Research Council (CNR) - Institute of Marine Sciences (ISMAR), Genova, Italy2PE Imaging of Cancer Cells Using PEGylated Gold Nanorods as Biocompatible Con-trast Agent.

120

P2 Giulio Caracciolo1, Daniela Pozzi1, Anna Laura Capriotti2, Chiara Cavaliere2, Francesco Cardarelli3, Angelo Bifone3, Giuseppe Bardi3, Fabrizio Salomone3 and Aldo Laganà2

1 Department of Molecular Medicine, ‘Sapienza’ University of Rome, Viale Regina Elena, 324, 00161, Rome, Italy2 Department of Chemistry, ‘Sapienza’ University of Rome, P.le A. Moro 5, 00185, Rome, Italy,3 Center for Nanotechnology Innovation @NEST, Istituto Italiano di Tecnologia, Piazza San Silvestro 12, 56127 Pisa, ItalyProtein corona controls the uptake delivery mechanisms of lipid gene vectors in can-cer cell

123

P3 Tatjana Dramićanin, Lea Lenhardt, Ivana Zeković, Nasta Tanić and Miroslav D. DramićaninVinča Institute of Nuclear Sciences, University of Belgrade, P.O. Box 522, 11001 Belgrade, SerbiaBreast Cancer Detection by Support Vector Machine Classification of Synchronous Fluorescence Spectroscopy Data

128

P4 Ivana Zekovića, Lea Lehnardta*, Tatjana Dramićanina, Miroslav D. Dramićaninaa Vinča Institute of Nuclear Sciences, University of Belgrade, PO Box 522, 11001 Belgrade, SerbiaApplication of Supervised Self-Organizing Maps in Skin Melanoma Diagnosis by Total Synchronous Fluorescence Spectroscopy

144

P5 Elisabetta M. Caspani, Philip H. Crossley, Carolina Redondo-Garcia, Salvador MartinezLaboratory of Experimental Embryology, Instituto de Neurociencias UMH-CSIC, Alicante, SpainMalignopodium: a new player in glioma progression

*

P6 Roberto Cighettia, Matteo Piazzaa, Valentina Calabresea, Gaetana Damorea and Francesco Periaa Università degli Studi di Milano-Bicocca, Piazza della Scienza, 2 – 20126 – Milano, ItalyNew fluorescent molecular tools to investigate LPS recognition

124


P16 - P30 PageP16 Hanna Beckera,2, Amy Gladfelter3, Rudolf Oldenbourg1

1 Marine Biological Laboratory, Woods Hole, Massachusetts, USA2 Universität Konstanz, Konstanz, Germany3 Dartmouth College, Hanover, New Hampshire, USAQuantitative and high resolution imaging of fluorescence anisotropy is shedding light on septin architecture in the yeast bud neck

121

P17 Iván, Coto Hernández1,2, Giuseppe, Vicidomini1, Alberto, Diaspro1,2

1 Istituto Italiano di Tecnologia, Genova, Italia2 Universita Degli Studi di Genova, Genova, ItaliaGeneration Of Super Continuum Source And Its Application In Nanoscopy

125

P18 William F. Falco, Joelson Fernandes, Evaristo A. Falcão, Anderson R.L. CairesGrupo de Óptica Aplicada, Universidade Federal da Grande Dourados, Dourados, MS, BrazilEffects on Photosynthetic Activity of Plants Induced by Silver Nanoparticles: Moni-toring by Chlorophyll Fluorescence

130

P19 Joelson Fernandes1, William F. Falco1, Samuel L. Oliveira2, Anderson R.L. Caires1

1 Grupo de Óptica Aplicada, Universidade Federal da Grande Dourados, Dourados, MS, Brazil2 Grupo de Óptica e Fotônica, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, BrazilChanges on Photosynthetic Activity of Herbicide-tolerant Soybean Plants Induced by Glyphosate

131

P20 Jenni Karttunen1, Sari Mäntynen1, Heli Lehtivuori1, Janne Ihalainen1, Teemu Ihalainen1, Jaana K. H. Bamford1 and Hanna M. Oksanen2

1 Department of Biological and Environmental Science and Nanoscience Center, PO Box 35, 40014 University of Jyväskylä, Jyväskylä, Finland2 Institute of Biotechnology and Department of Biosciences, PO Box 56, 00014 University of Hel-sinki, Helsinki, FinlandProtein Localization and Interactions in Bacteria Studied with Confocal Microscopy and Fluorescence Lifetime

138

P21 Volkan KILIÇ, Gözde AYDOĞAN KILIÇ, Ahmet ÖZATAAnadolu University, Faculty of Science, Department of Biology, 26470, Eskişehir, TURKEYLight microscopic pathology in Common carp ( Cyprinus carpio L.,1758) from Sa-karya River (TURKEY)

140

P22 Lea Lenhardt1*, Ivana Zeković1, Tatjana Dramićanin1, Živoslav Tešić2, Dušanka Milojković-Opsenica2, Miroslav D. Dramićanin1

1 Vinča Institute of Nuclear Sciences, University of Belgrade, PO Box 522, 11001 Belgrade, Serbia2 Faculty of Chemistry, University of Belgrade, Studentski trg 12-16, 11000 Belgrade, Serbia.Assessment of Fluorescence and Infrared Spectroscopy Capabilities for the Determi-nation of Botanical Origin of Serbian Unifloral Honey

143


P16 - P30 PageP23 Daniela Pozzi1, Giulio Caracciolo1, Francesco Cardarelli2, Angelo Bifone2, Heinz

Amenitsch3

1 Department of Molecular Medicine, “Sapienza” University of Rome, Viale Regina Elena, 324, 00161, Rome, Italy2 Center for Nanotechnology Innovation @NEST, Istituto Italiano di Tecnologia, Piazza San Silvestro 12, 56127 Pisa, Italy3 Institute of Biophysics and Nanosystems Research, Austrian Academy of Sciences, Schmiedel-strasse 6, A-8042 Graz, AustriaConfocal microscopy makes visible cholesterol-mediated gene delivery mechanisms

152

P24 Liliia Rohoza, Tatyana Dyubko, Sergey Galchenko, Boris SandomirskyInstitute for Problems of Cryobiology and Cryomedicine fo the NAS of Ukraine, KharkivSpectrofluorimetric investigation of interactions of heart peptides with blood serum albumin

153

P25 Sebastian Tannert1, Peter Kapusta1, Alexander Glatz1, Christian Litwinski1, Uwe Ortmann1, Rainer Erdmann1, Katrin Hoffmann2, Ute Resch-Genger2

1 PicoQuant GmbH, Rudower Chaussee 29, 12489 Berlin, Germany, www.picoquant.com2 Federal Institute for Materials Research and Testing (BAM), Richard-Willstätter-Str. 11, 12489 Berlin, Germany) On the spectral sensitivity calibration of fluorescence spectrometers: Extension to the NIR, polarization and grating effects

154

P26 Anatoliy Tatarets1*, Yevgeniy Povrozin1, Olena Obukhova1, Iryna Fedyunya-yeva1, Ewald Terpetschnig2, Leonid Patsenker1,2

1 State Scientific Institution “Institute for Single Crystals”, NAS of Ukraine, 60 Lenin Ave., 61001 Kharkiv (Ukraine)2 SETA BioMedicals, LLC, 2014 Silver Ct East, Urbana, 61801, IL, USA,Novel Squaraine Probes for Proteins and Fluorescence Imaging Applications

155

P27 Francesco Urciuolo1, Giorgia Imparato1, Costantino Casale1, Fabio Formiggini1, Paolo Netti1,2

1 Center for Advanced Biomaterials for Health Care@CRIB, Istituto Italiano di Tecnologia, Largo Bar-santi e Matteucci n, 53 80125 Napoli2 Interdisciplinary Research Centre on Biomaterials (CRIB), University of Naples Federico II, P. Le Tecchio 80, 80125 Naples, ItalyCoupling Engineered Living Tissue with Multiphoton Tomography : towards in vitro understating of biological phenomena

156


P31 - P45 PageP31 William Dallari, Marco Allione, Elena Samoylova, Marco Scotto d’Abbusco,

Alberto DiasproNanophysics, Istituto Italiano di Tecnologia, via Morego, 30, 16163 GenovaPermanent and rewritable 3D optical data storage through multi-photon absorption

126

P32 Silvia Galiani1, Benjamin Harke1, Giuseppe Vicidomini1, Gabriele Lignani2, Fabio Benfenati2, Hanako Tsushima2, Evelina Chieregatti2, Paolo Bianchini1, Alberto Diaspro1

1Nanophysics, Istituto Italiano di Tecnologia, Genoa, Italy2Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, Genoa, ItalyOptimization of a STED nanoscope

133

P33 Manuel Gunkela, Nina Beila, Jürgen Benekea, Slawek Mazura, Jürgen Rey-manna, Bastian Schumachera and Holger Erfleaa Viroquant-CellNetworks RNAi Screening Facility, BioQuant, University of Heidelberg, GermanyAutomated microscopy in RNAi screening experiments

135

P34 Timo Hakkarainen1, Tero Setälä1, Ari T. Friberg1,2,3

1 Department of Applied Physics, Aalto University, Box 13500, FI-00076 Aalto, Finland2 Department of Physics and Mathematics, University of Eastern Finland, Box 111, FI-80101 Joensuu, Finland3 Department of Microelectronics and Applied Physics, Royal Institute of Technology (KTH), Elec-trum 229, SE-164 40 Kista, SwedenNear-Field Imaging of Interacting Nano-Particles

136

P35 Christian Jüngst*and Andreas ZumbuschDepartment of Chemistry, University of Konstanz, 78457 Konstanz, GermanyLabel-free imaging of Lipid Droplets in living cells

137

P36 Marcelle Koenig1, Steffen Ruettinger1, Sebastian Tannert1, Thomas Schoenau1, Christian Litwinski1, Olaf Schulz2, Michael Wahl1, Marcus Sackrow1, Kristian Lauritsen1, Felix Koberling1, Robert Ros2, and Rainer Erdmann1

1 PicoQuant GmbH, Rudower Chaussee 29, 12489 Berlin, Germany, [email protected] Arizona State University, Lab Robert Ros, Physics Department, Tempe, AZ 85287-1504, USAPushing the boundaries of single molecule detection

141

P37 Zeno Lavagninoa1, Francesca Cella Zanacchi1, Alberto Diaspro1,2

1 Nanophysics, Istituto Italiano di Tecnologia, Via Morego 30,16163 Genova, Italia2 LAMBS- Dept. of Physics – University of Genoa, Via Dodecaneso 33, 16153 Genova, ItaliaThick Sample imaging and scattering effects: a phantom sample based characteriza-tion of Single Plane Illumination Microscopy performances

142

P38 Aimar Namma2, Andres Arend1, Marina Aunapuua,2

1 Department of Anatomy, University of Tartu, Ravila 19, 50411 Tartu, Estonia2 Department of Veterinary Medicine, Estonian University of Life Sciences, Fr Kreutzwaldi 62, 51014 TartuComputer assisted image analysis in the detection of expression of bone morphoge-netic proteins in the neural tube of human embryos

146

P39 Marco Zanella1†, Luca Ceseracciu2†, Roberta Ruffilli1, Alberto Diaspro2, Liber-ato Manna1

1 Nanochemistry, Istituto Italiano di Tecnologia, via Morego 30, 16163 Genova, Italy2 Nanophysics, Istituto Italiano di Tecnologia, via Morego 30, 16163 Genova, ItalyPolarized plasmonic absorption in micrometer long copper(I) selenide nanowires

158


P46 - P60 PageP46 Abebe Belay

Adama Science and Technology University, School of Natural Sciences, Department of Physics, P.O.Box, 1888, Adama, Ethiopia,The Hetero-Association of Caffeine (CA) with 5-Caffeoyl quinic Acid (5-CQA) and Ethidium Bromide (EB) in Aqueous Solution

122

P47 Mariagrazia Di Luca1*, Francesco Cardarelli2, Ranieri Bizzarri 1,3

1 NEST, Scuola Normale Superiore and Istituto Nanoscienze-CNR, Piazza San Silvestro 12 - 56127 Pisa2 Center for Nanotechnology Innovation @NEST, Istituto Italiano di Tecnologia, Piazza San Silvestro 12, 56127 Pisa3 Istituto di Biofisica - CNR, via Moruzzi 1, 56124 PisaFluorescent recombinant virus like particles as nano-carriers of sensors

127

P48 Sabato Fusco, Valeria Panzetta, Paolo Antonio NettiCenter for Advanced Biomaterials for Health Care@CRIB, Istituto Italiano di Tecnologia Largo Bar-santi e Matteucci, 53 80125 NapoliCell Mechanical Characterization by Particle Tracking Microrheology in Optical Mi-croscopy

132

P49 Gallus Lorenzo1*, Ferrando Sara1, Gambardella Chiara1, Amaroli Andrea1, Faimali Marco2, Veronica Piazza2, Bianchini Paolo3, Masini Maria Angela1

1 Dip. di Scienze della Terra, dell’Ambiente e della Vita (DiSTAV), Univ. di Genova2 Ist. di Scienze Marine, ICMM, CNR, Area di Genova3 Dipartimento di Nanofisica, Istituto Italiano di Tecnologia GenovaAquaporins in cyprid of barnacle Balanus amphitrite (=Amphibalanus amphitrite) (Cirripedia, Crustacea)

134

P50 Gözde Aydoağan KILIÇ, Volkan KILIÇ, Ahmet ÖZATAAnadolu University, Faculty of Science, Department of Biology, 26470, Eskişehir, TURKEYAutometallographic localization of heavy metals and immunoperoxidase detection of metallothionein induction in Common carp (Cyprinus carpio L.1758) from Sakarya River (TURKEY)

139

P51 Keurison F. Magalhães1,3, Anderson R.L. Caires1*, Margarete S. Silva2, Samuel L. Oliveira3

1 Grupo de Óptica Aplicada, Universidade Federal da Grande Dourados, Dourados, MS, Brazil2 Grupo de Estudo e Desenvolvimento de Materiais, Universidade Estadual de Mato Grosso do Sul, Dourados, MS,Brazil3 Grupo de Óptica e Fotônica, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, BrazilThe Role of Fatty Acid Methyl Esters on Optical Properties of Biodiesel

145

P52 Olena Obukhova1*, Anatoliy Tatarets1, Olga Kolosova1, Iryna Fedyunyayeva1, Leonid Patsenker1,2, Ewald Terpetschnig2

1 State Scientific Institution “Institute for Single Crystals”, NAS of Ukraine, 60 Lenin Ave., 61001 Kharkiv (Ukraine)2 SETA BioMedicals, LLC, 2014 Silver Ct East, Urbana, 61801, IL, USAHighly Photostable Biomedical Markers Based on Thiosquarylium Dyes

147


P53 Silvio Panettieri1 Giovanni Signore2 Ranieri Bizzarri2

1 NEST, Istituto Nanoscienze - CNR, Piazza S. Silvestro 12, 56127 Pisa2 Center for Nanotechnology Innovation @NEST, Istituto Italiano di Tecnologia, Piazza San Silvestro 12, 56127 PisaSynthesis, stereochemical characterization, and photophysical features of an innova-tive 3-styryl coumarin acting as polarity/viscosity sensor for bioanalytical use

148

P54 Leonid Patsenker1,2*, Ewald Terpetschnig2, Anatoliy Tatarets1, Olena Obuk-hova1, Olga Kolosova1, Larysa Markova1, Inna Yermolenko1

1 State Scientific Institution “Institute for Single Crystals”, NAS of Ukraine, 60 Lenin Ave., 61001 Kharkiv (Ukraine).2 SETA BioMedicals, LLC, 2014 Silver Ct East, Urbana, 61801, IL, USA,Next Generation Fluorescent Dyes for Biomedical Applications

149

P55 Alessia Petrelli, Claudio Canale, Alberto Diaspro and Silvia DanteNanophysics Department, Fondazione Istituto Italiano di Tecnologia, Genova (Italy)Quantitative evaluation of cell adhesion on patterned surfaces

150

P56 Ferruccio Pisanello1,2*, Luigi Martiradonna1, Godefroy Leménager3, Alberto Bramati3, Massimo De Vittorio1,4

1 Center for Bio- Molecular Nanotechnologies @UNILE, Istituto Italiano di Tecnologia, 73010 Arne-sano (Lecce), Italy2 Center for Neuroscience and Cognitive Systems @UNITN, Istituto Italiano di Tecnologia, 38068 Rovereto (TN), Italy3 Laboratoire Kastler Brossel, Université Pierre et Marie Curie, CNRS UMR 8552, Ecole Normale Supérieure, 75005 Paris, France4 National Nanotechnology Laboratory, CNR- ‐Nano, Università del Salento, Dipartimento Ingegn-eria dell’innovazione, 73100 Lecce, ItalyAnisotropic colloidal nanocrystals as ultrastable polarized quantum emitters

151

P57 Kelly-Ann Vere, Joanna Richens and Paul O’SheaCell Biophysics Group, Institute of Biophysics, Imaging and Optical Science (IBIOS), School of Biol-ogy, University of Nottingham, UK NG7 2RDElectrostatic mapping of molecular interactions on the cell surface; identification of novel cell-surface receptor systems

157


Conference Sponsors

M I C R O S Y S T E M S

Diamond and Gold

OWLS Member AssemblyJuly 5th, 2012 in Genoa, Italy (17:30-19:00)

Porto Antico Congress Center – Magazzini del Cotone – Scirocco-Libeccio Room

AgendaExtra content: Lecture by Francesco Pavone (see program)

1. Discussion of the Agenda2. President’s Report3. Update of bylaws4. Elections

❚ Board ❙ President, Vice-President, Secretary, Treasurer ❙ OWLS Representative to ICO

❚ Regional Council Representatives ❙ Africa, North America, South America, Asia, Australia/New Zealand, Europe

❚ Standing Committees ❙ Bylaws and Regulatory Statutes; ❙ Technology Transfer, Standardization, and Technology Assessment; ❙ Finances; ❙ Education and Ethics

5. Meeting schedule ❚ OWLS 13 Location ❚ Co-Sponsoring

6. Treasurer’s report and decision of formal approval ❚ Financial Report

7. New Members8. Publications9. Dues10. Committee Reports11. Miscellaneous

Prof. Alberto DiasproPresident of the International Society on Optics Within Life Sciences (OWLS)

Documents

ORGANISING COMMITTEE 2012.pdf · OWLS • Genoa, 4-6 July 2012 • Program • Page 5 OWLS 2012 Tuesday, July 3rd - 7.00pm Inaugural Ceremony Sala del Maggior Consiglio, Palazzo Ducale