Propiedades Fisico Quimicas Quinoa

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    Food Science and Technology International

    DOI: 10.1177/1082013203009002006

    2003; 9; 101Food Science and Technology InternationalH. Dogan and M. V. Karwe

    Physicochemical Properties of Quinoa Extrudates

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    Physicochemical Properties of Quinoa Extrudates

    H. Dog an1 andM.V. Karwe2,*

    1TUBITAK Marmara Research Center, Food Science and Technology Research Institute,P.O. Box 21, Gebze/Kocaeli, 41470, Turkey

    2Food Science Department, Rutgers University, 65 Dudley Road, New Brunswick, NJ, 08901 USA

    Response surface methodology (RSM) was used to analyse the effect of temperature, screw speed, and

    feed moisture content on physicochemical properties of quinoa extrudates. A three-level, three-variable,

    Box-Behnken design of experiments was used. The experiments were run at 1624% feed moisture content,

    130170C temperature, and 250500 rpm screw speed with a fixed feed rate of 300 g/min. Second order

    polynomials were used to model the extruder response and extrudate properties as a function of process

    variables. Responses were most affected by changes in feed moisture content and temperature, and to a

    lesser extent by screw speed. Calculated specific mechanical energy (SME) values ranged between 170

    402 kJ/kg which were lower than those observed for other cereals, most likely due to high (7.2%) fat

    content of quinoa. High levels of feed moisture alone, and in combination with high temperature, resulted

    in poor expansion. The best product, characterised by maximum expansion, minimum density, high degree

    of gelatinization and low water solubility index, was obtained at 16% feed moisture content, 130 C die

    temperature, and 375 rpm screw speed, which corresponds to high SME input. It was demonstrated that

    the pseudo-cereal quinoa can be used to make novel, healthy, extruded, snack-type food products.

    Key Words: quinoa, extrusion cooking, physico-chemical properties

    INTRODUCTION

    Quinoa (Chenopodium quinoa Willd.) is a disc shaped

    small seed that looks like a cross between sesame seed

    and millet. It is a crop that has been grown in South

    American countries for centuries and has many poten-

    tially beneficial properties such as resistance to cold

    (Becker and Hanners, 1990; Coulter and Lorenz, 1991a;

    Prakash et al., 1993). It can be grown in poor soil and athigh altitude (Ng et al., 1994). The edible seed of the

    quinoa plant has been called both a pseudo-cereal and a

    pseudo-oilseed because of its unique nutritional profile.

    It has been recently identified to have promising

    potential to overcome worlds food shortage (Ahamed

    et al., 1996). The seeds have protein quality comparable

    to that of whole dry milk in terms of balanced amino

    acid composition (Ng et al., 1994). Quinoa protein is

    rich in lysine, methionine and cysteine (Becker and

    Hanners, 1990). Thus, it is a good complement for

    legumes, which are often low in methionine and

    cysteine. Some types of wheat come close to matching

    protein content of quinoa, but cereals such as corn andrice generally have less than half the protein content of

    quinoa. In addition, quinoa is a relatively good source

    of vitamin E, and several of the B vitamins (Ruales and

    Nair, 1993; Ahamed et al., 1996). It also has desirable

    fatty acid composition, and high levels of calcium, iron

    and phosphorous (Ruales and Nair, 1993; Przybylsk

    et al., 1994) which make it a unique food source.

    The Aztecs and Incas credited quinoa with medicina

    properties including lowering blood cholesterol, improv-

    ing glucose tolerance and reducing insulin requirements

    (Guzman-Maldonado and Paredes-Lopez, 1998). In

    recent years, scientific information supporting the

    health benefits of quinoa has accumulated (Guzman

    Maldonado and Paredes-Lopez, 1998). Quinoa contains

    significant amounts of flavonoids and phenolic acids

    and a number of structurally diverse saponins (Ridout

    et al., 1991; Gee et al., 1993; Ng et al., 1994

    Masterbroek et al., 2000). Saponins can help lower

    cholesterol blood levels, inhibit growth of cancer cells

    eliminate digestive toxins, and strengthen the immune

    system (Arditi et al., 2000). Phenolic derivatives act as

    natural antimicrobial agents. They have been proven to

    be very good antioxidants, scavenging free radicals and

    providing metal chelating activities. Polyphenols havebeen implicated in health benefits, such as prevention of

    cancer and cardiovascular diseases.

    This unique added chemical composition makes

    quinoa an ideal candidate to be further studied for

    establishing it as a functional food. Processing o

    traditional grains like quinoa into products that deliver

    nutritive as well as physiologically active components

    represents a major opportunity for food processors

    catering to the health-food market.

    Extensive studies on extrusion processing of cereals

    such as corn and wheat, to generate ready-to-ea

    *To whom correspondence should be sent(e-mail: [email protected]).Received 11 July 2002; revised 18 December 2002.

    Food Sci Tech Int 2003;9(2):010114 2003Sage PublicationsISSN: 1082-0132DOI: 10.1177/108201303033940

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    breakfast cereals and snacks, have been carried out

    (Chinnaswamy and Hanna, 1990; Case et al., 1992; Cai

    and Diosady, 1993; Guha et al., 1997). The only study

    reported in literature on extrusion of quinoa (Coulter

    and Lorenz, 1991a, b) is about the nutritional, sensory

    and physical characteristics of quinoa-corn grit blends

    (up to 30 : 70 ratio) extruded at 1525% feed moisture

    content, 100150C, 100200 rpm screw speed, and at1 : 1 and 3 : 1 compression ratios on a Brabender

    Plasticorder single-screw extruder. Although the pro-

    ducts extruded at 15% moisture content and a 3 : 1

    compression ratio had a greater expansion, lower

    density and lower shear strength, addition of quinoa

    to corn grit resulted in a general decrease in product

    quality and an increase in extrusion rate under all

    processing conditions.

    In our research we focused on the investigation of

    processability of quinoa flour by twin-screw extrusion

    and the evaluation of physicochemical properties of

    extruded quinoa in comparison to unprocessed grains.

    This paper treats the effect of feed moisture content,die temperature, and screw speed on process and

    product responses during twin-screw extrusion of

    quinoa flour.

    MATERIALS AND METHODS

    Material

    Quinoa seeds (Chenopodium quinoa Willd) were

    obtained from Quinoa Corporation (Torrance, CA)

    and milled into flour using a Fitz Mill (Model D).

    Proximate Analysis

    For the proximate composition analysis of quinoa

    flour the following methods were used (AACC, 1984).

    Moisture: oven drying at 103C (method no. 4415A).

    Ash: calcination at 550C (method no. 0801)

    Lipids: defatting in a soxhlet apparatus with petro-

    leum ether (method no. 3010)

    Protein: micro Kjeldahl (N 6.25) (method no. 4613)

    CHOfiber: by the difference.

    Amylose content was determined by the method

    proposed by Chrastil (1987). The method is based on

    spectrophotometric measurement of the intensity of blue

    color formed due to complex formation between

    amylose and iodine.

    Extrusion

    Extrusion experiments were carried out on a twin-

    screw extruder (ZSK-30, from Krupp Werner &

    Pfleiderer, Ramsey, New Jersey). The extruder has two

    co-rotating, self-wiping screws (30.7 mm diameter,

    4.7 mm channel depth, and 878mm processing length;

    L/D 28.6) in a steel barrel with five zones. Each zone is

    heated by resistive electric heaters and the temperature of

    each zone can be controlled independently. The screw

    configuration used in extrusion experiments consisted of

    forward conveying elements, mild mixing elements,

    kneading elements and reverse elements (Table 1). Die

    pressure was measured using a Dynisco pressure trans-

    ducer (TPT463E, Dynisco, Sharon, MA). The die had

    two circular orifices (3 mm diameter, 5 mm long). Quinoa

    flour was metered into the feed section of the extruder

    with a volumetric feeder (K-Tron Corp., Pitman, NJ).

    Water was injected into the feed section of the extruder

    immediately after the feed port using a triple action

    piston pump (US Electric Co., Milford, CT). Both the

    feeder and the pump were calibrated prior to extrusion

    runs to determine the set points required for desired massflow rates of quinoa flour and water, respectively.

    Throughput or the total mass flow rate (flour water)

    was kept constant at 300 g/min for all experiments.

    Temperatures at zones I, II, and III were set to room

    temperature, 80 and 120C, respectively, while the

    temperatures at zones IV and V were adjusted such that

    the desired die temperatures could be maintained.

    Table 1. Screw configuration used for quinoa extrusion.

    Extrusion zones*

    Feed zone

    (84mm)

    Zone I

    (196mm)

    Zone II

    (210mm)

    Zone III

    (178mm)

    Zone IV

    (98mm)

    Zone V

    (84mm)

    Die zone

    (28mm)SK 42/42 42/21 T 28/28 28/14 20/10 KB 45/5/14 14/14

    SK 42/42 42/42 28/28 KB 45/5/14 20/10 KB 45/5/14 LH 14/14

    42/42 IGEL 42 20/20 KB 45/5/20 14/14

    42/21 28/28 20/20 20/10 LH 14/14

    IGEL 42 28/28 20/20 20/10 14/14

    28/28 KB 45/5/28 20/20 20/10 14/14

    28/14 20/20 14/14

    28/14 KB 45/5/20 14/14

    20/10 LH

    20/10

    20/10

    *IGEL: mild kneading element. KB: kneading block. LH: left handed (reverse) element. SK: feed element.T: transition element.

    102 H. DOGAN AND M.V. KARWE

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    Experimental Design

    Response surface methodology was used to investi-

    gate the effects of extrusion conditions on the product

    and process responses of quinoa. Results from pre-

    liminary trials were used to select suitable extruder

    operating window. The independent variables con-

    sidered in this study were feed moisture content(1624% w.b.), die temperature (130170C), and

    screw speed (250500 rpm). A three-variable, three-

    level, Box-Behnken design (Table 2) was employed to

    determine the extrusion conditions. Experiments were

    randomized in order to minimize the systematic bias in

    the observed responses due to extraneous factors.

    Preparation of Samples

    Samples were collected under steady state conditions

    of pressure, torque and temperature. Immediately after

    extrusion, extrudates were cooled, packed into glass jars,

    flushed with nitrogen gas, sealed and kept refrigerated(5C) until analysis.

    Process Responses

    The ZSK-30 extruder is equipped with a torque

    indicator which shows % torque which is proportional

    to the current drawn by the drive motor. A reading

    of 100% torque corresponds to the max allowable

    torque of 172 Nm. The specific mechanical energy

    (SME) was calculated from the measured torque reading

    as follows (Godavarti and Karwe, 1997):

    SME kJ=kg

    Total torque (%) Friction torque (%) N 9:1

    100 500mf

    1

    The drive motor has a rated power of 9.1 kW at a

    rated screw speed of 500 rpm. The friction torque was

    measured with screws attached to the drive and the

    barrel empty.

    The determination of specific energy delivered

    (SED) to the extrudate is based on the energy balance

    (Figure 1) between the inlet and just before the exit at

    the die of the extruder under steady state conditionscomputed from the following equations,

    mfCpiTi QH QC ME mfCpo Tp 2

    QH QC

    mf

    ME

    mf Cpo Tp CpiTi 3

    SME and SED were measured from experimenta

    conditions and STE was calculated from Equation (4).

    STE SME SED 4

    Negative value of specific thermal energy (STE)

    indicates net cooling at the barrel.

    Product Responses

    Extrudate samples used for determination of the

    degree of gelatinization (DG), water solubility index

    (WSI), and water absorption index (WAI), were dried at

    45

    C overnight to 45% moisture. Dried samples wereground and passed through 28-mesh sieve (590mm

    opening), and the flour samples were placed in glass

    jars and sealed. The method proposed by Birch and

    Priesty (1973) was used for determination of the DG

    The method was based on the monitoring of the

    complexation of iodine with amylose released due to

    starch gelatinization. The results reported are the mean

    of five measurements for each extrudate sample.

    Water solubility index and water absorption index of

    both unprocessed quinoa and extrudate samples were

    determined by the method of Anderson et al. (1969) with

    Table 2. Experimental design for extrusion of quinoa.

    Coded Levels Actual Levels

    X1 X2 X3 M(% wb) T(C) S (rpm)

    1 1 0 24 170 375

    1 1 0 24 130 375

    1 1 0 16 170 375

    1 1 0 16 130 375

    0 1 1 20 170 500

    0 1 1 20 170 250

    0 1 1 20 130 500

    0 1 1 20 130 250

    1 0 1 24 150 500

    1 0 1 24 150 250

    1 0 1 16 150 500

    1 0 1 16 150 250

    0 0 0 20 150 375

    0 0 0 20 150 375

    0 0 0 20 150 375Figure 1. Schematic diagram showing the barrel oextruder and various energy flows.

    Quinoa Extrudates 103

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    some modifications. WSI was calculated as follows:

    WSI g water soluble matter

    g dry sample 5

    For the calculation of WAI the total solids in the

    original sample were corrected for the loss of solubles inthe supernatant and WAI was expressed as,

    WAI g water absorbed

    g dry sample 1 soluble fraction 6

    Product density (e) was measured by volumetric

    displacement method as described by Hicsasmaz and

    Clayton (1993). Glass beads of 0.5 mm diameter

    (Biospec Products, Inc., Bartlesville, OK) were used as

    displacement medium. Density of glass beads was

    determined as 1550 kg/m3, then the density of extrudates

    was calculated as

    e We

    Wgbgb 7

    The e values were obtained from five random

    samples for each extrusion condition, with three

    replications.

    The sectional expansion index (SEI) of extrudate was

    measured as the ratio of the diameter of the extrudate to

    that of the die. The extrudate diameter was measured

    with a digital Vernier caliper and the results were

    expressed as the average of hundred measurements on

    each condition. The longitudinal (LEI) and volumetric

    expansion (VEI) indices were calculated according to

    Alvarez-Martinez et al. (1988).

    Textural properties of extrudates were measured using

    TA-XT2 texture analyser (Stable Micro Systems, UK).

    A three-point bend rig with a support length (bridge) of

    30mm and a rounded plate probe (15 mm 5mm,

    D 5 mm) exerting force in the middle of bridge were

    used to test extrudates in the bend mode (Zasypkin and

    Lee, 1998). The test speed was 2 mm/s and the full load

    scale was 50 kg. Data were processed with an XT-RA

    Dimension software package (Stable Micro Systems,Haslemere, Surrey, UK). The hardness of dry extrudates

    was measured as the peak force offered by the sample

    during cutting. Breaking strength (N/mm2) was calcu-

    lated as the peak breaking force (N) divided by the

    cross-sectional area (mm2) calculated for each extrudate

    sample. The reported values are the averages of 15

    measurements.

    The color of ground unprocessed quinoa and

    extrudate samples was measured in triplicate using

    Minolta Chroma Meter (CR-210) in terms of Hunter

    Lab values (L,a,b), where L represents lightness with 0

    for dark and 100 for bright, a represents the extent of

    green colour in the range from 100 to 0 and red in the

    range 0 to 100, b quantifies blue colour in the range

    from 100 to 0 and yellow in the range from 0 to 100.

    The total colour change (E) was then calculated as

    Effiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffi ffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffi ffiffiffiffiffiffiL 2 a 2 b 2

    q 8

    where L LL0, ia aa0, and b bb0; the

    subscript 0 indicates initial colour values before

    processing.

    Analysis of Data

    Process responses (SME, SED, STE, SME/SED) and

    product responses (iE, e, WAI, WSI, DG, SEI, LEI,

    VEI, hardness and breaking strength) obtained as a

    result of the proposed experimental design were

    subjected to regression analysis in order to assess theeffects of feed moisture content, extrusion temperature

    and screw speed. Second-order polynomials of the form

    yi b0 X3i1

    biXi X3i1

    X3ji

    bijXiXj 9

    were fitted to the independent variables and were

    computed by using SAS (version 8.1) statistical package,

    where Xi, XiXi and XiXj are linear, quadratic, and

    interaction effect of the input variables which influence

    the response y, respectively, and b0, bi, and bij are themodel constants to be determined. All crosscorrelations

    between the process and product responses themselves

    were also assessed. The response surface plots for these

    models were plotted as a function of two variables, while

    keeping the third variable constant at its intermediate

    value.

    RESULTS AND DISCUSSION

    Composition of grain could vary with variety and

    growing conditions, even though experimental results forcomposition (Table 3) agreed with previous data (Becker

    and Hanners, 1990; Coulter and Lorenz, 1990; Guzman-

    Maldano and Paredes-Lopez, 1998; Koziol, 1992;

    Prakash et al., 1993; Ruales and Nair, 1993). The

    quinoa seeds used in this study have high crude protein,

    crude fat and ash than common cereals, such as rice and

    corn. Extrudates of widely different physical structure

    were obtained by twin-screw extrusion of quinoa flour at

    differentcombinations ofprocessingparameters (Table2).

    Regression analyses of the physicochemical properties of

    quinoa extrudates (Table 4) indicated that all the second

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    order polynomial models correlated well with the mea-

    sured data and were statistically significant (p < 0.05).

    Process Responses

    Calculated SME values ranged between 170 and

    402 kJ/kg. The regression analysis results (Table 4)

    revealed that temperature (T), feed moisture content

    (M) and screw speed (S) had linear and significant

    (p

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    Table 4. Results of regression analysis (calculated on coded levels where all independent variables a

    Levels

    Degree of

    Geltn

    Density

    (kg/m3)

    Sectional

    Expansion

    Index

    Longitudinal

    Expansion

    Index

    Volumetric

    Expansion

    Index

    Water

    Solubility

    Index

    (g/g)

    Water

    Absorption

    Index

    (g/g) iE

    Hardness

    (N)

    Breaking

    Strength

    (N/mm2)

    Spe

    Mecha

    Ene

    (kJ/

    C 0.781*** 246.7*** 2.75*** 0.630** 4.78*** 0.202*** 6.03*** 21.35*** 3.62*** 0.068*** 258.

    M 0.046*** 88.1*** 0.43*** 0.090ns 1.67*** 0.025*** 0.57*** 1.88*** 0.19ns 0.041*** 37.9

    T 0.058*** 30.4*** 0.73*** 0.650*** 0.99** 0.017*** 0.14** 0.84** 1.04*** 0.028*** 59.3

    S 0.034*** 38.0*** 0.15* 0.010ns 0.61ns 0.021*** 0.20*** 0.53ns 0.17ns 0.016** 51.

    M M 0.033** 57.9*** 0.24** 0.296ns 0.33ns 0.026*** 0.33*** 1.49** 0.46* 0.017* 15.6

    T T 0.037*** 34.6** 0.39*** 0.711** 0.96ns 0.031*** 0.15* 2.67*** 0.62** 0.021** 23.

    S S 0.041*** 38.2** 0.04ns

    0.364ns

    0.77ns

    0.009** 0.19** 0.25ns

    0.34ns

    0.001ns

    9.M T 0.015ns 4.0ns 0.17ns 0.218ns 0.92ns 0.002ns 0.53*** 0.10ns 0.07ns 0.017* 11.

    M S 0.015ns 18.8ns 0.10ns 0.038ns 0.05ns 0.003ns 0.02ns 0.76ns 0.32ns 0.006ns 9.

    T S 0.009ns 26.8** 0.02ns 0.098ns 0.66ns 0.011** 0.06ns 0.10ns 0.15ns 0.004ns 7.

    R2 0.98 0.98 0.98 0.90 0.89 0.99 0.98 0.96 0.94 0.97 0.

    F 28.28 28.68 24.65 5.25 4.59 102.6 27.13 13.97 9.48 16.14 21.

    Sig. F 0.001 0.001 0.001 0.041 0.052 0.000 0.001 0.005 0.012 0.003 0.

    C: model constant; M , Tand S: linear effects of moisture content, die temperature and screw speed, respectively. M M, T Tand S S: quadratic effects of moistrespectively;.M T, M S and T S: interaction effects of moisture content and die temperature, moisture content and screw speed, and die temperature and screw**significant at p

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    (T), screw speed (S) and moisture (M), and the

    quadratic effects of screw speed (S S) and tempera-

    ture (T T) had the highest impact, significant at

    p

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    In general, low feed moisture and high product

    temperature have been found to increase the DG in

    extrusion of starchy materials (Bhattacharya and

    Hanna, 1987; Cai and Diosady, 1993). However, in the

    presence of lipids, extrusion temperature required for

    maximum DG was in the intermediate range. Lower

    temperatures compensated for the decrease in melt

    viscosity due to lipids (Dog an, 2000). In high lipidcontaining cereals like quinoa, excessive feed moisture

    acts as a secondary lubricant which prevents the

    achievement of appropriate development or cooking of

    dough by shear induced disruption. In this study, DG

    was found to be highly correlated (0.81, p

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    decreased with increasing temperature (Figure 5), which

    can be explained by the negative effect of temperature

    on the elasticity of extrusion cooked melts (Launay and

    Lisch, 1983). This result is in agreement also with the

    work of Ilo et al. (1999). On the other hand, longitudinal

    expansion appeared to be extensively favored by lower

    melt viscosity at higher temperature and higher moisture

    level (Figure 6).Volumetric expansion index, the multiplication pro-

    duct of SEI and LEI, was affected only by feed moisture

    content (p

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    giving a minimum value at about 140C die tempera-

    ture, 19.1% feed moisture, and 425 rpm screw speed.

    The mechanical properties of extruded products can

    be described either by compressive deformation or by

    breaking strength (Colonna et al., 1989). Breaking

    strength is the measure of the strength of cell wall

    which is expected to affect the texture and sensory

    crispiness of the extruded product (Chen et al., 1991).Breaking strength was found to be highly correlated

    with SEI (0.85,p

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    feed moisture content due to the fact that high thermal

    and mechanical energy inputs favour starch dextriniza-

    tion (Figure 9). Low WSI values were observed at

    intermediate to low temperature levels at all processing

    conditions. The increase in WSI with increasing

    temperature was consistent with the results reported

    for oat extrudates (Singh and Smith, 1997). The

    WSI decreased with the increase in moisture. Similareffects of decreasing moisture on WSI have been

    reported earlier for starch, maize grits, wheat and pea

    flour (Della Valle et al., 1994; Kirby et al., 1988).

    Minimum WSI of 0.186 g/g was achieved at 18.3% feed

    moisture content, 140C die temperature, and 192 rpm

    screw speed.

    The poor correlation between WSI and most of the

    process and product responses (Table 5) may be

    explained by the fact that WSI includes the opposing

    effects of starch dextrinization and the molecular level

    interactions between degraded components, which may

    not be favoured at the same condition. An increase in

    the amount of dextrinized starch during extrusion

    cooking results in an increase in WSI. However

    molecular interactions between degraded starch, pro

    tein, and lipid components, which in turn lead to anincrease in molecular weight, may decrease the solubi-

    lity, thus WSI. Moreover, according to the mode

    proposed by Gomez and Aguilera (1984) for starch

    degradation during extrusion cooking, three pure states

    i.e., raw, gelatinized, and dextrinized, of starch exis

    together. Due to different states in which starch is found

    in extrudates, some granules may be underprocessed

    while some others may be overprocessed or dextrinized

    According to the same model, dextrinization can be

    considered to take place together with or right after

    adequate gelatinization. According to our experimental

    results, thermal and mechanical input seemed to be

    enough for sufficient starch gelatinization but not severeenough to favour starch dextrinization. SME values

    were not high enough to cause dextrinization, which in

    turn increases WSI. This is also evident by the high

    correlation coefficient between SME and DG (0.81

    p 0.10).

    Water absorption index depends on the availability of

    hydrophilic groups and on the gel formation capacity of

    the macromolecules (Gomez and Aguilera, 1983). It is a

    measure of damaged starch together with protein

    denaturation and new macromolecular complex forma-

    tions. WAI of extrudates ranged between 4.45 and

    6.72 g/g which was significantly higher than that o

    (1.69g/g) unprocessed quinoa. The regression analysis

    (Table 4) showed that the linear effect of feed moisture

    content (M), and the interaction effect of die tempera-

    ture and moisture content (T M) were highly sig

    nificant on WAI. Singh and Smith (1997) reported an

    increase in WAI with the increase in moisture and

    temperature during extrusion of oats, which is in

    agreement with our experimental results (Figure 10)

    WAI had poor correlations with almost all process and

    product responses except product density (Table 5). This

    is an expected result since it includes the effect of starch

    gelatinization, protein denaturation and molecular levelcrosslinking reactions which are not always favoured at

    the same conditions.

    Colour

    Colour is an important quality parameter since i

    reflects the extent of chemical reactions and degree of

    cooking or degradation that take place during extrusion

    cooking. In this study, E represents the total colour

    difference compared to the colour of unprocessed

    quinoa. Higher E means darker products with more

    Figure 9. Effect of feed moisture content, die tem-perature and screw speed on water solubility index(WSI, g soluble matter/g dry sample).

    Quinoa Extrudates 111

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    intense yellow and red colour. The total colour change

    in extruded products ranged between 15.5 and 23.7

    (Table 4). Quadratic effect of temperature (T T) and

    the linear effect of feed moisture content (M) were

    found to have the highest contribution to total colour

    change. Low feed moisture content and high tempera-

    ture increased the total colour change possibly due toincreased extent of browning reactions under this

    condition (Figure 11). Although, the screw speed was

    not a significant parameter (Table 4), at low screw

    speeds a slight increase in colour change observed due to

    longer residence times which might increase the extent of

    chemical reactions.

    In summary, because the high lipid and low amylose

    contents, extrusion cooking of quinoa required proces-

    sing conditions that provide high shear environ-

    ment indicated by high SME values which disrupts

    starch granules. Extrusion cooking conditions that

    produced quinoa products with desirable expansion

    characteristics were at low moisture, low temperature

    and medium screw speed within the range of our process

    variables.

    NOMENCLATURE

    Cp heat capacity (kJ/kg.K)

    LEI longitudinal expansion index (dimensionless)

    ME rate of mechanical energy input (dissipa-

    tion) (W)

    mf total mass flow rate (kg/s)

    N screw speed (rpm)

    QC rate of energy removal by the cooling sys-

    tem (W)

    QH rate of heat generation (W)

    SED specific energy delivered (kJ/kg)

    SEI sectional expansion index (dimensionless)

    Figure 10. Effect of feed moisture content, dietemperature and screw speed on water absorptionindex (WAI, g H2O/g dry sample).

    Figure 11. Effect of feed moisture content, die

    temperature and screw speed on total color change(E).

    112 H. DOGAN AND M.V. KARWE

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    SME specific mechanical energy (kJ/kg)

    STE specific thermal energy (kJ/kg)

    Ti inlet temperature of the product (C)

    To outlet temperature of the product (C)

    Td die temperature or product temperature (C)

    VEI volumetric expansion index (dimensionless)

    WAI water absorption index (g water absorbed/g dry

    sample)We weight of extrudate (kg)

    Wgb weight of glass beads displaced by the extru-

    dates (kg)

    WSI water solubility index (g water soluble matter/g

    dry sample)

    E total colour change

    e density of extrudate (kg/m3)

    gb density of glass beads (kg/m3)

    ACKNOWLEDGEMENTS

    This is publication No. D 01544-01-01 of the NewJersey Agricultural Experiment Station supported by

    State funds and the Center for Advanced Food

    Technology (CAFT). The Center for Advanced Food

    Technology is a New Jersey Commission on Science

    and Technology Center. Author H. Dog an acknowl-

    edges the financial support from TUBITAK Marmara

    Research Center, Food Science and Technology Res-

    earch Institute, Turkey.

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