Engineering BioBrick vectors from BioBrick parts

Reshma P Shetty, Drew Endy and Thomas F Knight Jr

Outline

Background Synthetic Biology Terms

Method Current Research Discussion

Synthetic Biology

What is Synthetic Biology? A Method

Milestones Recombinant DNA PCR(Polymerase Chain Reaction) Automated Sequencing Automated Construction Standards Abstractions

-Write

-------------------Read

Last 30 years

Synthetic Biology

Synthetic Biology (Cont.) Systems

Devices

Parts

DNA

Terms

Vector Plasmid Polymerase Restriction enzyme Ligase

Method

Goal Standardize the BioBrick vectors’ assembly

process Maintain

Cloning site Selection marker High copy orgin Terminators flanking the cloning site Verification primer annealing sites

Process Build new BioBrick vectors’ backbone Standard assembly for new BioBrick

Method(Cont.)

Basic BioBrick

Method(Cont.)

Build new BioBrick vectors’ backbone

Method(Cont.)

Standard assembly for new BioBrick

Method(Cont.) Check

Cloning site Selection marker High copy orgin Terminators flanking the cloning site Verification primer annealing sites

Current Research

More than 200 parts

Some Systems

Discussion

Wetware Biological film Digital counting machine

Interdisciplinary research

Computer aided design